CN102641119A - Animal tail suspension experiment device - Google Patents
Animal tail suspension experiment device Download PDFInfo
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- CN102641119A CN102641119A CN2012101252513A CN201210125251A CN102641119A CN 102641119 A CN102641119 A CN 102641119A CN 2012101252513 A CN2012101252513 A CN 2012101252513A CN 201210125251 A CN201210125251 A CN 201210125251A CN 102641119 A CN102641119 A CN 102641119A
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Abstract
The invention relates to an animal tail suspension experiment device, comprising a base, a bracket, a tail clamper, a sensing locator and a control device; a bottom end of the bracket is fixed on the base, an upper end is connected with the tail clamper; the sensing locator is electrically connected with the control device; the sensing locator comprises a mounting shaft and an U-shaped rotating handle; a lower end of the mounting shaft is fixed on the base; the U-shaped rotating handle is mounted on the upper end of the mounting shaft; two ends of the U-shaped rotating handle raise up to be U-shaped; an end of the U-shaped rotating handle is equipped with an infrared signal transmitting tube and a located signal source, the other one end is equipped with an infrared signal receiving tube matched with the infrared signal transmitting tube; the infrared signal transmitting tube, the located signal source and the infrared signal receiving tube are controlled by the control device. The animal tail suspension experiment device provided by the invention has the advantages that proper clamping force is provided, man-made interference is removed, anti-jamming capability is strong, operations are simple, and use is convenient.
Description
Technical field
The present invention relates to the outstanding tail experimental provision of a kind of animal.
Background technology
The outstanding tail experiment of animal is widely used in the development of antidepressant drug, analeptic drug, downern and relevant health product; This mice tail end that clips makes its suspension and then struggle, abandons, struggles, abandons, and progressively getting into the motionless state of distinctive depression is a kind of method of estimating the said medicine curative effect.But experimentation often runs into trouble and error in judgement on the certain operations; Like pinch off animal tail, artificially counting and interfering signal etc., develop the bionical folder tail of a kind of tool function, the outstanding tail experiment of head displacement detection method automatic detection device that capacity of resisting disturbance is stronger for this reason.As a kind of brand-new automatization detection means of this experiment, what in the industry cycle obtain applying has very promising prospects.
Similar experiment in the industry cycle has the method that adopts written notes and playing back videos, stress detection method, infrared thermal release electric detection method etc. at present, write down notes and the playing back videos method too backward; The stress detection method is better to the fierce outstanding tail mice effect of struggling, but undesirable to the detection effect of slow struggle, and in addition, the weight of experiment mice, struggle intensity etc. bring difficulty all can for zeroing and judgement when detecting; It is too high to requirements such as self power supply and testing environments that infrared thermal release electric detects rule, and capacity of resisting disturbance is relatively poor, and the extremity that are subject to not think the process of struggling in the experiment such as wave at the interference of factor.
A general character when outstanding tail mice struggles: or nose forward or yaw to side or crispatura upwards, its head of often struggling must be more from original position.It is more scientific and reasonable to utilize this head displacement signal to judge whether outstanding tail mice is just locating the struggle behavior, and the interference of factor such as receive that extremity are waved is also less relatively, the principle development according to this of coherent detection instrument.
Summary of the invention
In order to solve the easy pinch off animal of folder tail instrument tail in the present outstanding tail experiment, artificially to count, be subject to interferential problem, the outstanding tail experimental provision of animal that the present invention proposes and a kind of suitable chucking power is provided, gets rid of interference from human factor, capacity of resisting disturbance is strong.
Animal of the present invention is hanged the tail experimental provision, it is characterized in that: comprise base, support, folder cercoids, sensing localizer, control device, described support bottom is fixed on the described base, and the upper end is connected with described folder cercoids; Described sensing localizer is electrically connected with described control device;
Described sensing localizer comprises installation shaft, U type rotary handle; The lower end of described installation shaft is fixed on the described base; Described U type rotary handle is installed in the upper end of described installation shaft; The two ends of described U type rotary handle are upturned and are the U type, and an end of described U type rotary handle is installed by the infrared signal transmitting tube and positioning signal source, the other end are installed the infrared signal receiving tube that matees with described infrared signal transmitting tube; Described infrared signal transmitting tube, positioning signal source, infrared signal receiving tube all receive the control of described control device;
Described control device comprises composite control apparatus, display screen, and described composite controller comprises main control chip, TTL functional circuit, analog amplify circuit, and described composite controller is electrically connected with described display screen; The control knob of described display screen receives the control of described main control chip; After described control knob configured, the described main control chip described sensing localizer of commands for controlling of giving an order began operate as normal; Described infrared signal transmitting tube, infrared signal receiving tube carry out signal processing with the main control chip that the signal of catching is transferred to described control device, and the information after will changing through described display screen shows;
Described folder cercoids comprises cantilever beam, sleeve pipe, slide block, spring, clamping nut, and described casing pipe sleeve is connected on described support upper end, realizes telescopic location through regulating the lateral fixed screw of described sleeve pipe; Described cantilever beam one end and described sleeve pipe side are fixed, and the end section of the other end is provided with fixed slip bar and screw rod, and described slide block set is connected on described slide bar and the screw rod; Described spring housing is connected on the screw rod between described slide block and the described cantilever beam, and is hidden in the corresponding spring eye of described slide block; Fixing symmetrically a pair of semilune latex tubing forms the folder uropore between described slide block and the described cantilever beam; Described clamping nut is screwed onto on the described screw rod that passes from slide block.
Described U type rotary handle is axis of symmetry with the installation shaft, and the two ends height is identical.
Described positioning signal source is close to the top of described infrared signal transmitting tube and is installed.
Described main control chip is 51 serial general single chip microcomputer AT89S51.
Described control knob and switch comprise counting zero clearing/section counting the Show Button, countdown setting, SR, start button, laser switch, on and off switch; Described countdown setting adds button and forms by button and time are set.
Described cantilever beam and described sleeve pipe are light rigid materials.
The thickness of described cantilever beam is greater than 8mm.
The length of described slide block is less than the length of described screw rod.
Described clamping nut, fixed slip bar, screw rod and fixed screw are stainless steel, and described clamping nut surface knurling, described fixed screw are the ingot head.
Described folder uropore upper end bore is less than the lower end bore, and the symmetric gradient of facing of described folder uropore is 2 degree.
Described counting zero clearing/section counting shows that button is used by on-off control exchange, and an end is used for stored count and counts back zero, sets and can return zero in running time at any time, but can continue counting; The other end is used to show each fragmentation count value and total count value, presses once, and first demonstration segment value (1,2,3,4,5) that the left side is 4 by once showing this section count value, shows total count value more at last.Not stored count under this pattern.
Described countdown setting adds two buttons and forms by button and time is set, and can be provided with 5 sections continuously, and it is 99 minutes and 60 seconds that total time is set; When being provided with, earlier by button being set, first demonstration section of setting (1 that the right is 4; 2,3,4; 5), by button is set, 4 on the right can begin to add button by the time this section period is set again; 5 sections are provided with after the completion again by button is set, show the total time number is set, so repetitive cycling.
Described rest button is used for countdown and returns zero, and wait is reset.
Described beginning button control counting and countdown, to countdown to zero, control device lock count value, timing time is got back to settings, control device waits for that zero clearing of next counting or counting show or countdown setting or reset or the arrival of commencing signal; This hour counter just can begin stored count in the time of need waiting for the arrival of next commencing signal.
Opening or closing of described laser switch control laser sight.
Described on and off switch control main power, when described on and off switch was opened, counting showed to be zero entirely with timing, control device wait countdown setting, the input of signal such as began.
During use, open total power switch (220V, 50Hz), counting showed with timing and was zero this moment, laser sight is punctation a little less than the demonstration of E place, adds button by button and time is set respectively, sets the needed experimental period of each section; Get mice, outstanding tail reversal of the natural order of things, the forehead direction is being clamped Mus tail apart from mice tail end 1～1.5cm place with the folder cercoids towards the experimenter, is adjusted to infrared information transmitting tube and infrared information receiving tube two places, makes red some positive alignment mice of laser forehead (two are on the upper side), closes laser; Press start button, this hour counter begins accumulative total from the mice struggle times that infrared sensor measured, and timer begins countdown; Countdown is to zero the time, and enumerator stops counting and locking current number, the timer settings before the countdown that resets, and one time experimentation finishes.Press start button again, enumerator begins stored count on the basis of former numerical value again, and timer begins a countdown of repetition by last the setting again; Counting zero clearing and timing reset unrestricted, but repeatable operation in the experiment, but zero clearing midway or reset and can not make the count value pinning; Counting after once experiment finishes is a total count value, and each fragmentation count value can be checked by section counting demonstration button.
The invention has the beneficial effects as follows: suitable chucking power is provided, gets rid of interference from human factor, capacity of resisting disturbance is strong, simple to operate, easy to use.
Description of drawings
Fig. 1 is overall structure figure of the present invention
Fig. 2 is a control flow chart of the present invention
Fig. 3 is circuit control principle figure of the present invention
Fig. 4 is the front view of folder cercoids of the present invention
Fig. 5 is the vertical view of folder cercoids of the present invention
Fig. 6 is the exploded view of folder cercoids of the present invention
Fig. 7 is experiment flow figure of the present invention
Fig. 8 utilizes device of the present invention to carry out the experimental result picture (-: healthy group ■-■: depression group) of the outstanding tail experiment of animal
The specific embodiment
Further specify the present invention below in conjunction with accompanying drawing
With reference to accompanying drawing:
Embodiment 1
Animal of the present invention is hanged the tail experimental provision, comprises base 1, support 2, folder cercoids 3, sensing localizer 4, control device 5, and described support 2 bottoms are fixed on the described base 1, and the upper end is connected with described folder cercoids 3; Described sensing localizer 4 is electrically connected with described control device 5;
Described sensing localizer 4 comprises installation shaft 41, U type rotary handle 42; The lower end of described installation shaft 41 is fixed on the described base 1; Described U type rotary handle 42 is installed in the upper end of described installation shaft 41; The two ends of described U type rotary handle 42 are upturned and are the U type, and an end of described U type rotary handle 42 is installed by the infrared signal transmitting tube and positioning signal source, the other end are installed the infrared signal receiving tube that matees with described infrared signal transmitting tube; Described infrared signal transmitting tube, positioning signal source, infrared signal receiving tube all receive the control of described control device 5;
Described control device 5 comprises composite control apparatus, display screen, and described composite controller comprises main control chip, TTL functional circuit, analog amplify circuit, and described composite controller is electrically connected with described display screen; The control knob of described display screen receives the control of described main control chip; After described control knob configured, the described main control chip described sensing localizer of commands for controlling of giving an order began operate as normal; Described infrared signal transmitting tube, infrared signal receiving tube carry out signal processing with the main control chip that the signal of catching is transferred to described control device, and the information after will changing through described display screen shows;
Described folder cercoids 3 comprises cantilever beam 31, sleeve pipe 32, slide block 33, spring 34, clamping nut 35, and described sleeve pipe 32 is socketed in described support 2 upper ends, through regulating the location that described sleeve pipe 32 lateral fixed screws 321 are realized sleeve pipe 32; Described cantilever beam 31 1 ends and described sleeve pipe 32 sides are fixed, and the end section of the other end is provided with fixed slip bar 311 and screw rod 312, and described slide block 33 is socketed on described slide bar 311 and the screw rod 312; Described spring 34 is socketed on the screw rod 312 between described slide block 33 and the described cantilever beam 31, and is hidden in the corresponding spring eye 331 of described slide block; Fixing symmetrically a pair of semilune latex tubing forms folder uropore 36 between described slide block 33 and the described cantilever beam 31; Described clamping nut 35 is screwed onto on the described screw rod 312 that passes from slide block 33.
Described rotary handle 42 is an axis of symmetry with installation shaft 41, and the two ends height is identical.
Described positioning signal source is close to the top of described infrared signal transmitting tube and is installed.
Described main control chip is 51 serial general single chip microcomputer AT89S51.
Described control knob and switch comprise counting zero clearing/section counting the Show Button, countdown setting, SR, start button, laser switch, on and off switch; Described countdown setting adds button and forms by button and time are set.
Described cantilever beam 31 is light rigid materials with described sleeve pipe 32.
The thickness of described cantilever beam 31 is greater than 8mm.
The length of described slide block 33 is less than the length of described screw rod 312.
Described clamping nut 35, fixed slip bar 311, screw rod 312 and fixed screw 321 are stainless steel, and described clamping nut 35 surface knurlings, described fixed screw 321 are the ingot head.
Described folder uropore 36 upper end bores are less than the lower end bore, and the described folder uropore 36 symmetric gradients of facing are 2 degree.
Described counting zero clearing/section counting shows that button is used by on-off control exchange, and an end is used for stored count and counts back zero, sets and can return zero in running time at any time, but can continue counting; The other end is used to show each fragmentation count value and total count value, presses once, and first demonstration segment value (1,2,3,4,5) that the left side is 4 by once showing this section count value, shows total count value more at last.Not stored count under this pattern.
Described countdown setting adds two buttons and forms by button and time is set, and can be provided with 5 sections continuously, and it is 99 minutes and 60 seconds that total time is set; When being provided with, earlier by button being set, first demonstration section of setting (1 that the right is 4; 2,3,4; 5), by button is set, 4 on the right can begin to add button by the time this section period is set again; 5 sections are provided with after the completion again by button is set, show the total time number is set, so repetitive cycling.
Described rest button is used for countdown and returns zero, and wait is reset.
Described beginning button control counting and countdown, to countdown to zero, control device lock count value, timing time is got back to settings, control device waits for that zero clearing of next counting or counting show or countdown setting or reset or the arrival of commencing signal; This hour counter just can begin stored count in the time of need waiting for the arrival of next commencing signal.
Opening or closing of described laser switch control laser sight.
Described on and off switch control main power, when described on and off switch was opened, counting showed to be zero entirely with timing, control device wait countdown setting, the input of signal such as began.
During use, open total power switch (220V, 50Hz), counting showed with timing and was zero this moment, laser sight is punctation a little less than the demonstration of E place, adds button by button and time is set respectively, sets the needed experimental period of each section; Get mice, outstanding tail reversal of the natural order of things, the forehead direction is being clamped Mus tail apart from mice tail end 1～1.5cm place with the folder cercoids towards the experimenter, is adjusted to infrared information transmitting tube and infrared information receiving tube two places, makes red some positive alignment mice of laser forehead (two are on the upper side), closes laser; Press start button, this hour counter begins accumulative total from the mice struggle times that infrared sensor measured, and timer begins countdown; Countdown is to zero the time, and enumerator stops counting and locking current number, the timer settings before the countdown that resets, and one time experimentation finishes.Press start button again, enumerator begins stored count on the basis of former numerical value again, and timer begins a countdown of repetition by last the setting again; Counting zero clearing and timing reset unrestricted, but repeatable operation in the experiment, but zero clearing midway or reset and can not make the count value pinning; Counting after once experiment finishes is a total count value, and each fragmentation count value can be checked by section counting demonstration button.
Embodiment 2
Utilize the device among the embodiment 1; Carry out the outstanding tail experiment of white mice; According to animal behavior: white mice is just by outstanding tail the time, and it is comparatively fierce to struggle, reuse laser red-point aiming mice forehead when treating to hang one's head relatively calmness after the several seconds; Being provided with in advance under the countdown situation, can experimentize by start button.
According to Animal behaviour: outstanding tail mice is subject to the surrounding enviroment influence and struggles, and for this reason, during experiment, whole experiment examination frame shields with the C shape box of a uncovered.
30 routine white mice experiments on the instrument of finally succeeding in developing, have been carried out; White mice body weight 15～25g, body tail length overall 150～190mm, the experimental period of single white mice is set at 5～25min; Get 15 healthy mices; Do the outstanding tail experiment of 5～25min, every section is provided with 5min, and the white mice that other gets 15 depressed modelings of warp compares experiment with healthy group.Outstanding tail experimental result by the visible 30 routine white mice of Fig. 8.
See that from experimental result the struggle times obvious difference of two treated animals after grasping the animal rule and testing skill, just can make full use of this experimental apparatus and carry out the outstanding tail experiment of animal.
The described content of this description embodiment only is enumerating the way of realization of inventive concept; Should not being regarded as of protection scope of the present invention only limits to the concrete form that embodiment states, protection scope of the present invention also reach in those skilled in the art conceive according to the present invention the equivalent technologies means that can expect.
Claims (10)
1. animal is hanged the tail experimental provision, and it is characterized in that: comprise base, support, folder cercoids, sensing localizer, control device, described support bottom is fixed on the described base, and the upper end is connected with described folder cercoids; Described sensing localizer is electrically connected with described control device;
Described sensing localizer comprises installation shaft, U type rotary handle; The lower end of described installation shaft is fixed on the described base; Described U type rotary handle is installed in the upper end of described installation shaft; The two ends of described U type rotary handle are upturned and are the U type, and an end of described rotary handle is installed by the infrared signal transmitting tube and positioning signal source, the other end are installed the infrared signal receiving tube that matees with described infrared signal transmitting tube; Described infrared signal transmitting tube, positioning signal source, infrared signal receiving tube all receive the control of described control device;
Described control device comprises composite control apparatus, display screen, and described composite controller comprises main control chip, TTL functional circuit, analog amplify circuit, and described composite controller is electrically connected with described display screen; The control knob of described display screen receives the control of described main control chip; After described control knob configured, the described main control chip described sensing localizer of commands for controlling of giving an order began operate as normal; Described infrared signal transmitting tube, infrared signal receiving tube carry out signal processing with the main control chip that the signal of catching is transferred to described control device, and the information after will changing through described display screen shows;
Described folder cercoids comprises cantilever beam, sleeve pipe, slide block, spring, clamping nut, and described casing pipe sleeve is connected on described support upper end, realizes telescopic location through regulating the lateral fixed screw of described sleeve pipe; Described cantilever beam one end and described sleeve pipe side are fixed, and the end section of the other end is provided with fixed slip bar and screw rod, and described slide block set is connected on described slide bar and the screw rod; Described spring housing is connected on the screw rod between described slide block and the described cantilever beam, and is hidden in the corresponding spring eye of described slide block; Fixing symmetrically a pair of semilune latex tubing forms the folder uropore between described slide block and the described cantilever beam; Described clamping nut is screwed onto on the described screw rod that passes from slide block.
2. like the outstanding tail experimental provision of claim 1 animal, it is characterized in that: described U type rotary handle is axis of symmetry with the installation shaft, and the two ends height is identical.
3. like the outstanding tail experimental provision of claim 2 animal, it is characterized in that: described positioning signal source is close to the top of described infrared signal transmitting tube and is installed.
4. like the outstanding tail experimental provision of claim 1 animal, it is characterized in that: described main control chip is 51 serial general single chip microcomputer AT89S51.
5. like the outstanding tail experimental provision of claim 1 animal, it is characterized in that: described control knob and switch comprise counting zero clearing/section counting the Show Button, countdown setting, SR, start button, laser switch, on and off switch; Described countdown setting adds button and forms by button and time are set.
6. like the outstanding tail experimental provision of claim 1 animal, it is characterized in that: described cantilever beam and described sleeve pipe are light rigid materials.
7. like the outstanding tail experimental provision of claim 6 animal, it is characterized in that: the thickness of described cantilever beam is greater than 8mm.
8. like the outstanding tail experimental provision of claim 7 animal, it is characterized in that: the length of described slide block is less than the length of described screw rod.
9. like the outstanding tail experimental provision of claim 8 animal, it is characterized in that: described clamping nut, fixed slip bar, screw rod and fixed screw are stainless steel, and described clamping nut surface knurling, described fixed screw are the ingot head.
10. like the outstanding tail experimental provision of claim 9 animal, it is characterized in that: described folder uropore upper end bore is less than the lower end bore, and the symmetric gradient of facing of described folder uropore is 2 degree.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101252513A CN102641119B (en) | 2012-04-25 | 2012-04-25 | Animal tail suspension experiment device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012101252513A CN102641119B (en) | 2012-04-25 | 2012-04-25 | Animal tail suspension experiment device |
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| CN102641119A true CN102641119A (en) | 2012-08-22 |
| CN102641119B CN102641119B (en) | 2013-11-13 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103800093A (en) * | 2014-03-11 | 2014-05-21 | 南京医科大学附属南京儿童医院 | Tail suspending device for laboratory mice |
| CN104138265A (en) * | 2013-05-08 | 2014-11-12 | 上海交通大学医学院 | Device for preventing climbing on tail in animal tail suspension test |
| CN104274157A (en) * | 2014-09-24 | 2015-01-14 | 深圳先进技术研究院 | Fixing device and photoacoustic tomography system with same |
| CN106706869A (en) * | 2017-01-22 | 2017-05-24 | 浙江中医药大学 | Multichannel testing device for vertical rotation persistence of mice |
| CN109906950A (en) * | 2019-04-10 | 2019-06-21 | 中国人民解放军第二军医大学 | The useless property modeling culture cage of animal hind leg |
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| JP2008212410A (en) * | 2007-03-05 | 2008-09-18 | Teiyoku Ri | Retaining appliance for laboratory animal |
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| CN106706869A (en) * | 2017-01-22 | 2017-05-24 | 浙江中医药大学 | Multichannel testing device for vertical rotation persistence of mice |
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| CN109906950A (en) * | 2019-04-10 | 2019-06-21 | 中国人民解放军第二军医大学 | The useless property modeling culture cage of animal hind leg |
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| CN102641119B (en) | 2013-11-13 |
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