The specific embodiment
Principle of the present invention is: the emulsifying agent raw material of choosing different lipophilic balance values (HLB); Carry out compound preparation; The HLB that makes compound emulsifying agent near feed fatty HLB; Make in the feed fat have in animal intestinal that good emulsifying is disperseed, stability, thereby improve the absorption efficiency of fat in the enteron aisle.
The present invention selects to have the triterpenoid saponin of higher HLB and carries out compound preparation with the sucrose fatty ester that hangs down HLB, makes that the emulsification threshold value is wideer, and the stability of fats emulsion strengthens.Secondly, triterpenoid saponin is by extracting in the plant, and security is good.
Animal and fowl fodder of the present invention is used the compound fat emulsifying agent, mainly is made up of triterpenoid saponin, sucrose fatty ester and starch, and wherein, the quality proportioning of triterpenoid saponin, sucrose fatty ester and starch is 50 ~ 70:30 ~ 40:0 ~ 20.
Preferably, the quality proportioning of said triterpenoid saponin, sucrose fatty ester and starch is 55:35:10.
The raw material triterpenoid saponin is extracted by tea seed, and the HLB value is 14.
The HLB value of raw material sucrose fatty ester is 5.
Animal and fowl fodder of the present invention is used the compound fat emulsifying agent, and its preparation process is following:
(1) takes by weighing triterpenoid saponin, sucrose fatty ester and starch respectively by quality proportioning 50 ~ 70:30 ~ 40:0 ~ 20;
(2) triterpenoid saponin, sucrose fatty ester and starch are mixed;
(3) packing.
Above-mentioned steps (2) adopts agravic type mixer.Step (3) is distributed into 1kg, 5kg.
These article are stored in cool place, dry place after preparation.
These article suggestion use amount is added compound fat emulsifying agent 100 ~ 500g of the present invention in the mixed feed per ton.
Describe the present invention in detail according to embodiment below, it is more obvious that the object of the invention and effect will become.
Embodiment of the invention raw material sources can be following, but all be not limited thereto:
1, triterpenoid saponin: manufacturer, the clear Bioisystech Co., Ltd of the Hunan Chinese.Be the tea seed extract, triterpenoid saponin content >=80%, HLB value 14, food-grade.
2, sucrose fatty ester: manufacturer, Liuzhou Ai Gefu food science and technology limited company.Sucrose-fatty ester content >=90%, HLB value 5, food-grade.
3, starch: manufacturer, Huzhou prospect medicine company chemistry Co., Ltd.Medical auxiliary materials can be water-soluble.
Embodiment 1:A kind of animal and fowl fodder is used the compound fat emulsifying agent, raw material consumption and compound method
(1) takes by weighing triterpenoid saponin, sucrose fatty ester and starch respectively by quality proportioning 55:35:10;
(2) triterpenoid saponin, sucrose fatty ester and starch are mixed;
(3) be distributed into 1kg, 5kg.
Embodiment 2:A kind of animal and fowl fodder is used the compound fat emulsifying agent, raw material consumption and compound method
(1) takes by weighing triterpenoid saponin, sucrose fatty ester and starch respectively by quality proportioning 50:40:10;
(2) triterpenoid saponin, sucrose fatty ester and starch are mixed;
(3) be distributed into 1kg, 5kg.
Embodiment 3:A kind of animal and fowl fodder is used the compound fat emulsifying agent, raw material consumption and compound method
(1) takes by weighing triterpenoid saponin, sucrose fatty ester and starch respectively by quality proportioning 68:30:2;
(2) triterpenoid saponin, sucrose fatty ester and starch are mixed;
(3) be distributed into 1kg, 5kg.
Embodiment 4:Animal and fowl fodder of the present invention is used the compound fat emulsifying agent, makes an addition to corn+dregs of beans+soya-bean oil type high fat diet, to the influence test of meat chicken production performance
1, materials and methods
1.1, the experimental basis daily ration
Prepare for fryer nutritional need standard with reference to AA company commodity.Daily ration 1 is used for the 1-14d feeding of broiler stage; Daily ration 2 is used for 15-28d and raises the stage; Daily ration 3 is used for 29-42d and raises the stage.Daily ration prescription, main trophic level are seen table 1.
1.2, feeding experiment design
The 1d commodity are divided into 4 groups at random for AA fryer 432 plumages.Every group 108 plumage established 3 repetitions for every group, and each repeats 36 plumages.The control group fed basal diet, 3 test group are added compound fat emulsifying agent 200,250,300mg/kg respectively in basal diet.The compound fat emulsifying agent is used the compound fat emulsifying agent for the animal and fowl fodder of instance 1 preparation of the present invention.
Table 1: test diet recipe and trophic component %
Daily ration is formed |
Daily ration 1 |
Daily ration 2 |
Daily ration 3 |
Trophic component |
Daily ration 1 |
Daily ration 2 |
Daily ration 3 |
Corn |
55.7 |
58.2 |
61.8 |
Metabolizable energy (MJ/kg) |
12.30 |
12.88 |
13.31 |
Dregs of beans |
35.5 |
32.5 |
28.0 |
Crude protein |
20.18 |
19.03 |
17.50 |
Soybean oil |
4.0 |
5.0 |
6.0 |
Crude fat |
6.75 |
7.82 |
8.84 |
Calcium monohydrogen phosphate |
1.8 |
1.6 |
1.5 |
Calcium |
0.99 |
0.87 |
0.80 |
Stone flour |
1.4 |
1.2 |
1.1 |
Available phosphorus |
0.46 |
0.43 |
0.40 |
Salt |
0.44 |
0.40 |
0.40 |
Lysine |
1.00 |
0.93 |
0.93 |
Methionine |
0.16 |
0.10 |
0.08 |
Methionine |
0.48 |
0.41 |
0.37 |
Lysine |
0 |
0 |
0.10 |
Cystine |
0.35 |
0.33 |
0.3 |
Premix |
1.0 |
1.0 |
1.0 |
? |
? |
? |
? |
Annotate: add VA 10000IU, VD in every kg basal diet
32000IU, VE 25IU, VK
32.5mg, VB
12.0mg, VB
26.0mg, VB
63.0mg, VB
120.02mg, nicotinic acid 30mg, pantothenic acid 15mg, folic acid 1.0mg, biotin 0.1mg.Cu?10mg,Fe?80mg,Mn?120mg,Zn?110mg,Se?0.5mg,I?0.3mg。
1.3, the test chicken feeding and management
Repeat to be one with each and independently raise fence, take up an area of 3.5 square metres, hassock material ground flat is supported.The daylighting on each hurdle, temperature, temperature, ventilation condition basically identical.Test chicken free choice feeding, drinking-water.24 illumination during 1-3d, 4d plays natural lighting.Carry out management work such as conventional eqpidemic disease immunity, sterilization between feeding period.
1.4, data acquisition
Repeating with each is unit, writes down feed intake weekly, and survey 14,28,42d be body weight on an empty stomach.Calculate body weight, weightening finish, feed intake and the material anharmonic ratio of each growth phase.
1.5, statistical analysis
Result data carries out one-way analysis of variance with SPSS13.0 software, with LSD method multiple ratio.Result data is represented with
± SE.
2, result of the test
The result sees table 2.Stage daily gain index.Compare with control group, each test group 1-14d improves 15.1% (P<0.05), 13.1% (P<0.05) and 16.4% (P<0.01) respectively; 3 test group differences are not remarkable.15-28d, test group improves 8.5% (P>0.05), 12.1% (P<0.05) and 44.1% (P<0.01) respectively.29-42d tests 2 groups and improves 11.4% (P<0.05), and it is not remarkable to test 1,3 group of difference.The 42d body weight is tested each component and you can well imagine high by 5.8% (P>0.05), 13.2% (P<0.01) and 21.6% (P<0.01).
Table 2: the emulsifying agent addition is to the g that influences of each stage weightening finish of fryer, feed intake and feed conversion rate
Annotate: with identical letter is arranged between line data, group difference is remarkable (P >=0.05) not; The different persons of letter, significant difference (P<0.05).
Feed intake and material anharmonic ratio.1-14d, feed intake does not have significant difference between group; The material anharmonic ratio reduces by 9.2% (P<0.01), 6.1% (P<0.05) and 6.1% (P<0.05) respectively.15-28d tests 3 groups of feed intakes and improves 30.7% (P<0.01); The material anharmonic ratio reduces by 0.6% (P>0.05), 4.1% (P>0.05) and 9.5% (P<0.01) respectively.Between 29-42d, the test group feed intake improves 7.3% (P>0.05), 20.0% (P<0.01), 10.1% (P<0.01) respectively; Test 1 group, 2 groups material anharmonic ratioes and improve 7.4% (P<0.01), 5.3% (P<0.05) respectively.
The result shows, adds the compound fat emulsifying agent in the feed, has obviously improved the speed of growth of fryer.Adding 42 days bodies of 150,200 gram test group brings up again high more than 13%.Before the compound fat emulsifying agent obviously reduces growth of meat chicken, the material anharmonic ratio in mid-term.Test group later stage material anharmonic ratio is higher than control group, thinks it is because test group later stage body weight is excessive, growth period inconsistent causing.
Embodiment 5:Animal and fowl fodder of the present invention is used the compound fat emulsifying agent, makes an addition to corn+dregs of beans type daily ration is supported part utilization rate to the yellow chicken production performance of middling speed, daily ration influence test
1, materials and methods
1.1, experimental animal and basal diet
Test chicken is a middling speed type yellow-feather broiler.The 1-14d jumpbogroup is raised.During 15d, choose body weight, 360 consistent plumages of body condition, public mother half and half is as test chicken.
With reference to national broiler fodder standard, combine this kind to recommend nutritional requirements to carry out the daily ration preparation.Daily ration 1 is used for 15-28d and raises the stage; Daily ration 2 is used for 29-42d and raises the stage.Daily ration prescription, main trophic level are seen table 3.
Table 3: basal diet prescription and trophic component %
The daily ration prescription |
Daily ration 1 |
Daily ration 2 |
Trophic component |
Daily ration 1 |
Daily ration 2 |
Corn |
67.20 |
71.20 |
Metabolizable energy (MJ/kg) |
11.80 |
12.20 |
Dregs of beans |
24.50 |
21.00 |
Crude protein |
18.50 |
17.20 |
Corn protein powder |
4.00 |
3.00 |
Crude fat |
3.15 |
2.85 |
Calcium monohydrogen phosphate |
1.50 |
1.70 |
Calcium |
0.92 |
0.84 |
Stone flour |
1.20 |
1.45 |
Available phosphorus |
0.40 |
0.38 |
Sodium chloride |
0.45 |
0.45 |
Lysine |
0.94 |
0.90 |
Methionine |
0.07 |
0.09 |
Methionine |
0.38 |
0.37 |
Lysine |
0.08 |
0.11 |
? |
? |
? |
50% Choline Chloride |
0.10 |
0.12 |
? |
? |
? |
Premix |
1.00 |
1.00 |
? |
? |
? |
Add up to |
100 |
100 |
? |
? |
? |
Annotate: premix is that every kg basal diet provides vitamin A 8000IU, vitamin D
31800IU, vitamin E 20IU, vitamin K
32.5mg, thiamine 2.0mg, riboflavin 6.0mg, Cobastab
63.0mg, Cobastab
120.02mg, nicotinic acid 30mg, pantothenic acid 15mg, folic acid 1.0mg, biotin 0.1mg.Copper 8mg, iron 70mg, manganese 90mg, zinc 80mg, selenium 0.3mg, iodine 0.3mg.
1.2, experimental design
Feeding experiment 14d360 plumage, public mother half and half is divided into 4 groups, i.e. 1 control group, 3 test group of adding the compound fat emulsifying agents.Every group 90 plumage established 3 repetitions for every group, and each repeats 30 plumages.Control group fed basal diet, test group add compound fat emulsifying agent 100,150,200 mg/kg respectively.The compound fat emulsifying agent is used the compound fat emulsifying agent for the animal and fowl fodder of instance 1 preparation of the present invention.
1.3, the test chicken feeding and management
To repeat is unit, and the subfield ground flat is supported, every hurdle condition basically identical.Test chicken free choice feeding and drinking-water, conventional eqpidemic disease is annotated vaccine immunity.
1.4, testing index and method
1.4.1, the production performance index
Repeating with each is unit, writes down feed intake weekly, and 28,42 d are body weight on an empty stomach.Add up each stage body weight, weightening finish and material anharmonic ratio index.
1.4.2, digestion trial
Fresh excreta is collected on each hurdle of secondary every day between 39-42d, adds watery hydrochloric acid fixed nitrogen.Finish the back and mix 70 ℃ of oven dry.Preserve the feed sample simultaneously.National Standard Method is measured dry, crude protein, crude fat, calcium, phosphorus, the acid-insoluble ash equal size in feed, the chicken manure, is the agent of endogenous index with the acid-insoluble ash, calculates the apparent digestibility (Yang Sheng, 1999) of feed nutrient.
Single nutrient digestion rate is calculated.Digestibility (%)=100-(in the ight soil in the content % ÷ feed content %) * (the insoluble content of ashes % of the insoluble content of ashes % of feed ÷ ight soil).
1.5, statistical analysis
Carry out the average of samples significance test of difference with SPSS13.0 software, with LSD method multiple ratio.Result data is represented with
± SE.
2, result
2.1 the compound fat emulsifying agent is to the growth of meat chicken Effect on Performance
Data are seen table 4, and test is compared with control group in earlier stage, and daily gain index difference not remarkable (P>0.05) is respectively organized in test.The later stage daily gain improves 12.8% (P<0.05), 6.2% (P>0.05) and 0.6% (P>0.05) respectively.Test the full phase and improve 9.1% (P<0.05), 4.4% (P>0.05) and 1.3% (P>0.05) respectively.
Compare with control group, in earlier stage, later stage material anharmonic ratio difference not significantly (P>0.05); The omnidistance material of test anharmonic ratio descends 0.6%, 3.2%, 1.3% respectively, and difference is not remarkable.
It is thus clear that emulsifying agent is under low dosage (100mg/kg), growth of meat chicken speed significantly improves; Strengthen consumption, gaining effect is not obvious on the contrary.Add emulsifying agent, material anharmonic ratio index has a declining tendency.
Table 4: the compound fat emulsifying agent is to the g that influences of meat chicken production performance
Annotate: between each line data, if the mark letter is different fully, expression significant difference (P<0.05).
2.2, the compound fat emulsifying agent is to the influence of diet nutrient composition apparent digestibility
Table 5: emulsifying agent is to the % that influences of feed nutrient digestibility
Project |
Control group |
Test 1 group |
Test 2 groups |
Test 3 groups |
Dry |
73.67±0.38
a |
76.92±1.55
b |
75.09±0.34
ab |
75.24±0.93
ab |
Crude fat |
84.33±0.59
a |
86.19±0.24
ab |
86.43±0.44
b |
87.14±0.48
b |
Crude protein |
50.13±0.71
a |
52.17±0.33
b |
52.61±2.19
b |
52.33±0.74
b |
Calcium |
49.00±0.73 |
50.71±0.52 |
49.57±0.53 |
50.87±0.51 |
Total phosphorus |
44.31±1.39 |
45.90±1.46 |
43.58±0.89 |
44.81±0.52 |
Data are seen table 5.Compare with control group, test diet dry substance digestibility improves 4.41% (P<0.05), 1.93% (P>0.05) and 2.13% (P>0.05) respectively; Crude fat improves 2.24% (P>0.05), 2.50% (P<0.05) and 3.25% (P<0.05) respectively; Thick protein improves 4.16%, 4.92% and 4.43% respectively, and difference is remarkable (P<0.05) all; Calcium, phosphorus index, no significant difference (P>0.05) is respectively organized in test.It is thus clear that, in corn-soybean meal diet, add the compound fat emulsifying agent, can improve the digestibility of daily ration of broiler dry, crude fat, thick protein, better with 100mg/kg, 150mg/kg interpolation group.
The foregoing description is used for the present invention that explains, rather than limits the invention, and in the protection domain of spirit of the present invention and claim, any modification and change to the present invention makes all fall into protection scope of the present invention.