CN102640846B - Selenium-rich mulberry leaf super micropowder feed and production method thereof - Google Patents

Selenium-rich mulberry leaf super micropowder feed and production method thereof Download PDF

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CN102640846B
CN102640846B CN 201210154600 CN201210154600A CN102640846B CN 102640846 B CN102640846 B CN 102640846B CN 201210154600 CN201210154600 CN 201210154600 CN 201210154600 A CN201210154600 A CN 201210154600A CN 102640846 B CN102640846 B CN 102640846B
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mulberry leaf
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CN102640846A (en
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何达崇
戴光明
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NANNING TORCH AGRICULTURE AND ANIMAL HUSBANDRY SCIENCE AND TECHNOLOGY Co Ltd
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NANNING TORCH AGRICULTURE AND ANIMAL HUSBANDRY SCIENCE AND TECHNOLOGY Co Ltd
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Abstract

The invention discloses a selenium-rich mulberry leaf super micropowder feed. The selenium-rich mulberry leaf super micropowder feed is characterized in that 5-20 parts by weight of selenium-rich mulberry leaf supermicro dry powder is added into common feed or fortified feed. The selenium-rich mulberry leaf super micropowder feed disclosed by the invention maintains the original nutritional properties of mulberry leaves and endow the mulberry leaves with a new nutrition health care function, so as to meet growth needs of livestock and reduce the incidence of diseases; meat of livestock fed with the selenium-rich mulberry leaf super micropowder feed is delicious in taste. A new approach is opened up for efficiently and cyclically utilizing mulberry.

Description

A kind of rich selenium Ultra-fine Powder of Mulberry Leaves feed and production method thereof
Technical field
The present invention relates to a kind of Ultra-fine Powder of Mulberry Leaves feed, particularly a kind of rich selenium Ultra-fine Powder of Mulberry Leaves feed and production method thereof, belong to biotechnology.
Background technology
Selenium is a kind of trace element that human body and animal are played an important role: 1, Organic Selenium can be removed interior free yl, get rid of vivotoxin, anti-oxidant, the generation that can effectively suppress lipid peroxide, prevent blood clot, remove cholesterol, strengthen immune function of human body; 2, selenium is the active component that consists of glutathione peroxidase, and it can prevent the beta Cell of islet Oxidative demage, makes its function normal, promotes the sugar metabolism, reduces blood sugar and glucose in urine, improves diabetic's symptom; 3, selenium can be protected retina, and the fineness of reinforcing glass body improves eyesight, prevents cataractous effect.4, selenium is the important element of keeping the heart normal function, the heart human body is had the effect of protection and reparation.The reduction of human body blood selenium level, can cause removing in body the hypofunction of free radical, cause the harmful substance deposition to increase, blood pressure rising, vascular wall thickening, blood vessel elasticity reduce, VPV is slack-off, send the oxygen function reduction, thereby the incidence of disease that brings out cardiovascular and cerebrovascular disease raises, yet supply Se scientific has effect preferably to prevention cardiovascular and cerebrovascular disease, hypertension, artery sclerosis etc.; 5, the adhesion of selenium and metal is very strong, can resist cadmium to the murder by poisoning of kidney, gonad and nervous centralis.The heavy metals such as the mercury of selenium in body, tin, thallium, lead are combined, form metallic selenium albumen compound and detoxify, toxin expelling; 6, prevent and treat hepatopathy, protection liver.But inorganic states selenium toxicity is large, and Organic Selenium toxicity is little; Mulberry leaf nutrient is abundant, contain various saccharides, vitamin and trace element, its nutritive value is better than most of leafy vegetable, and mulberry leaf contain the various bioactivators take alkaloids composition, flavones and flavonoid glycoside as representative: (1) carbohydrate-contain sucrose, fructose, glucose; (2) amino acids-contain 17 seed amino acids such as asparatate and glutamic acid, the high 2323mg/100g of its Glutamic Acid; (3) vitamins-every 100g mulberry leaf approximately contain the 4130IU vitamin A, 0.59 mg vitamin B1, and 1.35 mg vitamin B2s, 7.4 mg are square-bottomed bamboo basket Bu Su recklessly, 31.6 mg vitamin Cs; (4) trace element-mulberry leaf contain the trace element of the multiple needed by human such as zinc, copper, manganese, iron, and every 100g mulberry leaf are calcic 2699 mg approximately, potassium 3101 mg, magnesium 362 mg, iron 44.1 mg, sodium 39.9 mg, zinc 6.1 mg, copper 1.0 mg, manganese 27 mg etc.; (5) the most important characterization of alkaloid component-mulberry leaf studies and minute comprises DNJ (1-DNJ, N-methyl isophthalic acid-DNJ, 2-oxygen-α-D-galactolipin pyranoside-1-DNJ etc.; (5) flavones and flavonoid glycoside-the contain compounds such as rutin, rutin sophorin, Quercetin, isoquercitrin, Quercetin-3-three glucosides; (6) steroid constituents-contain cupreol, stigmasterol, campesterol etc.; (7) volatile oil component-contain acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, caproic acid, isocaproic acid, gaultherolin, guaiacol, adjacent phenyl methylcarbamate, a phenyl methylcarbamate, Eugenol etc.By use the little fertilizer of selenium (or selenium mineral powder) edible fungi residue (chaff) fermentation fertilizer in the growing of mulberry process, selenium is enriched to becomes Organic Selenium in mulberry leaf, produce again feeding rich selenium Ultra-fine Powder of Mulberry Leaves take the mulberry leaf of rich selenium as raw material, the fowl poultry is by taking food rich selenium Ultra-fine Powder of Mulberry Leaves, not only can raise by healthy fowl, but also can transfer to selenium again in fowl poultry meat, be replenishing selenium indirectly.From literature periodical, find no the feeding rich selenium Ultra-fine Powder of Mulberry Leaves of producing as raw material take the mulberry leaf of rich selenium like same item.
Summary of the invention
The objective of the invention is for above existing problems, and proposed to be applicable to a kind of rich selenium Ultra-fine Powder of Mulberry Leaves feed and the production method thereof that fowl is raiseeed healthy growth.
The present invention is achieved in that
The rich selenium Ultra-fine Powder of Mulberry Leaves of the present invention feed is the rich selenium mulberry leaf ultra micro dry powder that adds parts by weight 5-20 part in normal diet or fortified feed.
The production method of described rich selenium mulberry leaf ultra micro dry powder comprises the following steps:
(1), plantation is chosen and is rich in leaf protein, leaf is large, output is high mulberry tree breed;
(2), the edible fungi residue (chaff) that will gather in the crops mushroom (bacterium) takes off bag through machinery and smashs to pieces, then adds the little fertilizer of selenium or selenium mineral powder, bacillus subtilis live bacteria agent, the bacterium slag of every 100 kilograms adds the little fertilizer of selenium or selenium mineral powder 0.5-2 kilogram; Bacillus subtilis live bacteria agent 0.5-3 kilogram mixes, and builds heap, normal temperature fermentation 15-20 days, namely gets and contains selenium edible fungi residue (chaff) biological organic fertilizer;
(3), the plantation mulberry tree process use the selenium biological organic fertilizer for preparing, making selenium be enriched to mulberry leaf by bio-transformation becomes rich selenium mulberry leaf;
(4), results contain the mulberry leaf of rich selenium, in time carry out low-temperature vacuum drying and obtain dried mulberry leaf, then dried mulberry leaf are pulverized with micronizer, cross the 500-1000 mesh sieve, obtain to contain the feeding rich selenium Ultra-fine Powder of Mulberry Leaves of various bioactivators; I.e. rich selenium leaf protein, alkaloids, flavones and the flavonoid glycoside substance useful to growth of animal.
Above-described bacillus subtilis, the Latin formal name used at school, Bacillus subtilis, the direct cultivation with the method for textbook from institute of microbiology's purchase obtains, and can be used for herding, poultry, special animals and pet cultivation, also can be used in the production of sewage, industrial circulating water, septic tank, garbage disposal and fertilizer, can eliminate the animal waste stink, decompose the residue in animal house, degraded excrement slurry is eliminated stink; This product effectively degraded macromolecular material is lower-molecular substance, promotes the absorption of nutritional labeling, improves the utilization rate of feed, simultaneously can also suppress the disease microbial growth, improve the immunity of animal, strengthen the resistance against diseases of animal, reduce the generation of disease, improve survival rate.
The temperature that described low-temperature vacuum drying is chosen is 35-50 ℃; Adopt low-temperature vacuum drying can utilize vacuum that the moisture in mulberry leaf is further dry, excess Temperature can destroy the useful compositions such as vitamin in mulberry leaf, amino acid.
In above-mentioned, mulberry tree is used the method that contains selenium fertilizer, is to cut down in the annual summer to adopt root to execute after cutting down with the winter, and the summer is cut down 0.2 kilogram of rear every strain mulberry consumption, or 1000 kilograms of every mu of consumptions, and the winter is cut down 0.25 kilogram of rear every strain mulberry consumption, or 1250 kilograms of every mu of consumptions; The dried mulberry leaf of selenium content 5-20 mg/kg in rich selenium mulberry leaf.
The invention has the beneficial effects as follows:
1, the present invention can be used as poultry and livestock feed additive.
2, the result of use of the rich selenium Ultra-fine Powder of Mulberry Leaves of the present invention-add the rich selenium Ultra-fine Powder of Mulberry Leaves of 5%-15% to feed in the total feeds of livestock and poultry such as pig, chicken, duck, growth is normal, improves immunity, and disease reduces, the meat delicious flavour.
Along with the herding lean meat percentage improves constantly, but its quality obviously descends, and meat has caused people's concern.The improvement of meat is mainly from the improvement of kind and two aspect researchs of regulation and control of nutrition.Ma-han etc. add Organic Selenium in the basal diet of store pig, find that the lean meat percentage of trunk improves.The reports such as Wolter, Organic Selenium reduce fat thickness at back of pig, lean meat muscle area change.
Organic Selenium is owing to having the biological effects such as high-efficiency antioxidant, hypotoxicity, high-performance bio utilization rate, can provide green, safe pesticide herd product for people, it is with a wide range of applications at feedstuff industry, but also have some problems: (1) reaches the shortcomings such as equipment investment is large because the complicated rate of recovery of existing Organic Selenium synthesis route is low, therefore price is higher is several times to tens times of inorganic selenium, has limited its extensive use aborning; (2) Organic Selenium that uses in livestock-raising at present is mainly yeast selenium.Due to the intrinsic complexity of organism, how still make organic selenium product when producing in enormous quantities in Se content and chemical form stable, functional, be also that Present Domestic is produced the problem that Organic Selenium need to solve; (3) due to the difference of various places situation, animal feed selenium supplementary feeding amount there is no unified standard, so be still waiting further research in optimum addition and the interpolation stage of Organic Selenium; (4) not the same to the report of animal influence about Organic Selenium, some studies show that Organic Selenium plays positive role on animal produces, but there are some researchs not draw analog result, therefore need carry out a large amount of research works before the Organic Selenium of further carrying out in an all-round way.
The present invention utilizes selenium-enriched microbe to the synthetic probiotics of the stronger conversion capability of inorganic selenium, is that a green ecological is shifted to new management mechanisms, and also is conducive to taking full advantage of of resource simultaneously.Should from following several respects set about research, the development and utilization of Organic Selenium future: (1) takes full advantage of advanced molecular biology method, studies Organic Selenium in animal body and the collaborative and antagonism between other nutriment (vitamin E, phosphorus, iodine); (2) strengthen the exploitation of Organic Selenium preparation.The combination of the Organic Selenium in organism, especially selenium and organism (as plant and microorganism) active component, the application prospect aspect bio-pharmaceuticals is quite wide; (3) the rich selenium probiotics after fermenting-ripening contains a large amount of Secondary Metabolites of Microorganisms, and some product may have toxicity, need carry out the toxicity check, to guarantee food security.
The mechanism of action of Organic Selenium: selenium is the essential nutrient element of animals and plants body, along with people's deepening continuously to Organic Selenium research, find that it has biological function widely, now its present achievement in research is summarized as follows: 1, antioxidation for a long time, selenium is counted as a kind of important food source property antioxidant always, and its oxidation resistance is 50~500 times of vitamin E.The antioxidation of selenium removes by the enzymatic catalysis of glutathione peroxidase (GHS-Px) mainly that body endoperoxide and free radical realize.GHS-Px is the important endogenous antioxidant in organism; its major function is to remove unnecessary peroxide and the free radical that produces in organism; reduce the infringement of its cell membrane, the complete and function of Cell protection film, thus prevent imbalance and the death of cell function.To keeping animal health, improve meat, promote the reproductive performance important in inhibiting of animal.in animal body, about 30%~40% selenium is to exist with the glutathione peroxidase form, the numerous disease of animal all reduces relevant with the activity of glutathione peroxidase that scarce selenium causes with functional disturbance, this makes the GHS-Px activity be used as estimating the important indicator (Gao Dongyu etc. of body selenium situation and selemium nutrition requirement, 2009, NO1), improve immunity of organisms selenium to the nospecific immunity of body, humoral immunity, cellular immunity all has significant impact, closely related with the immune state of animal, be called as immunopotentiating agent (Wolter etc., 1999).The generation of selenium energy immune stimulatory globulin and antibody improves humoral immunity of organism, cellular immunity and nonspecific immunity function, is conducive to the cell toxin expelling.
Add bacillus subtilis in edible fungi residue, can faster decomposition of cellulose and protein, improve the amino acid content of fertilizer, more can improve fertilizer efficiency, promote the growth of mulberry tree, improve mulberry yield and quality.
The specific embodiment
Embodiment 1:
At first choose and be rich in leaf protein, the plantation of leaf is large, output is high mulberry tree breed, then the edible fungi residue (chaff) that will gather in the crops mushroom (bacterium) takes off bag through machinery and smashs to pieces, then add the little fertilizer of selenium or selenium mineral powder, bacillus subtilis live bacteria agent, the bacterium slag of every 100 kilograms adds the little fertilizer of selenium or selenium mineral powder 0.5-2 kilogram; Bacillus subtilis live bacteria agent 0.5-3 kilogram, mix, build heap, normal temperature fermentation 15-20 days, namely get and contain selenium edible fungi residue (chaff) biological organic fertilizer, in the process of plantation mulberry tree, use the selenium biological organic fertilizer for preparing, the annual summer is cut down and cuts down rear employing root with the winter and execute, summer is cut down 0.2 kilogram of rear every strain mulberry consumption, or 1000 kilograms of every mu of consumptions, the winter is cut down 0.25 kilogram of rear every strain mulberry consumption, or 1250 kilograms of every mu of consumptions; Make selenium be enriched to mulberry leaf by bio-transformation and become rich selenium mulberry leaf, results contain the mulberry leaf of rich selenium, the low-temperature vacuum drying that in time carries out below 45-40 ℃ is obtained dried mulberry leaf, then dried mulberry leaf are pulverized with micronizer, cross the 500-1000 mesh sieve, obtain to contain the feeding rich selenium Ultra-fine Powder of Mulberry Leaves of various bioactivators; I.e. rich selenium leaf protein, alkaloids, flavones and the flavonoid glycoside substance useful to growth of animal, the dried mulberry leaf of selenium content 5-10 mg/kg in rich selenium mulberry leaf, then join in normal diet or fortified feed, the parts by weight that add are the rich selenium mulberry leaf ultra micro dry powder of 5 parts, as feeding the poultry feeds such as chicken, duck, goose.Feed chicken, duck, goose with the poultry feed that the present embodiment obtains, have the hair color light, disease is few, healthy growth, and delicious meat contains Organic Selenium and improves 5-10 doubly than normal diet, and breeding potential improves more than 20%, and supply market is well received.
Embodiment 2:
At first choose and be rich in leaf protein, the plantation of leaf is large, output is high mulberry tree breed, then the edible fungi residue (chaff) that will gather in the crops mushroom (bacterium) takes off bag through machinery and smashs to pieces, then add the little fertilizer of selenium or selenium mineral powder, bacillus subtilis live bacteria agent, the bacterium slag of every 100 kilograms adds the little fertilizer of selenium or selenium mineral powder 0.5-2 kilogram; Bacillus subtilis live bacteria agent 0.5-3 kilogram, mix, build heap, normal temperature fermentation 15-20 days, namely get and contain selenium edible fungi residue (chaff) biological organic fertilizer, in the process of plantation mulberry tree, use the selenium biological organic fertilizer for preparing, the annual summer is cut down and cuts down rear employing root with the winter and execute, summer is cut down 0.2 kilogram of rear every strain mulberry consumption, or 1000 kilograms of every mu of consumptions, the winter is cut down 0.25 kilogram of rear every strain mulberry consumption, or 1250 kilograms of every mu of consumptions; Make selenium be enriched to mulberry leaf by bio-transformation and become rich selenium mulberry leaf, results contain the mulberry leaf of rich selenium, the low-temperature vacuum drying that in time carries out below 35-50 ℃ is obtained dried mulberry leaf, then dried mulberry leaf are pulverized with micronizer, cross 500-1000 mesh sieves, obtain to contain the feeding rich selenium Ultra-fine Powder of Mulberry Leaves of various bioactivators; I.e. rich selenium leaf protein, alkaloids, flavones and the flavonoid glycoside substance useful to growth of animal, the dried mulberry leaf of selenium content 5-10 mg/kg in rich selenium mulberry leaf, then join in normal diet or fortified feed, the parts by weight that add are the rich selenium mulberry leaf ultra micro dry powder of 10 parts, as feeding the sheep feed.The poultry feed that obtains with the present embodiment is fed sheep, have be not easy sick, healthy growth, delicious meat, breeding potential improves more than 20%.
Embodiment 3:
At first choose and be rich in leaf protein, the plantation of leaf is large, output is high mulberry tree breed, then the edible fungi residue (chaff) that will gather in the crops mushroom (bacterium) takes off bag through machinery and smashs to pieces, then add the little fertilizer of selenium or selenium mineral powder, bacillus subtilis live bacteria agent, the bacterium slag of every 100 kilograms adds the little fertilizer of selenium or selenium mineral powder 0.5-2 kilogram; Bacillus subtilis live bacteria agent 0.5-3 kilogram, mix, build heap, normal temperature fermentation 15-20 days, namely get and contain selenium edible fungi residue (chaff) biological organic fertilizer, in the process of plantation mulberry tree, use the selenium biological organic fertilizer for preparing, the annual summer is cut down and cuts down rear employing root with the winter and execute, summer is cut down 0.2 kilogram of rear every strain mulberry consumption, or 1000 kilograms of every mu of consumptions, the winter is cut down 0.25 kilogram of rear every strain mulberry consumption, or 1250 kilograms of every mu of consumptions; Make selenium be enriched to mulberry leaf by bio-transformation and become rich selenium mulberry leaf, results contain the mulberry leaf of rich selenium, the low-temperature vacuum drying that in time carries out below 45-50 ℃ is obtained dried mulberry leaf, then dried mulberry leaf are pulverized with micronizer, cross the 500-1000 mesh sieve, obtain to contain the feeding rich selenium Ultra-fine Powder of Mulberry Leaves of various bioactivators; I.e. rich selenium leaf protein, alkaloids, flavones and the flavonoid glycoside substance useful to growth of animal, the dried mulberry leaf of selenium content 5-10 mg/kg in rich selenium mulberry leaf, then join in normal diet or fortified feed, the parts by weight that add are the rich selenium mulberry leaf ultra micro dry powder of 20 parts, as pig feed.The feed that obtains with the present embodiment feeds pigs, and has the raising immunity of organisms, and disease is few, reduce heavy metal, lead, cadmium, arsenic and chromium residual quantity in vivo for example, healthy growth, delicious meat, the pig price of feeding than normal diet can improve 20-30%, and on market, supply falls short of demand.

Claims (4)

1. the production method of a rich selenium Ultra-fine Powder of Mulberry Leaves feed, it is characterized in that: this feed is the rich selenium mulberry leaf ultra micro dry powder that adds parts by weight 5-20 part in normal diet or fortified feed;
The production method of described rich selenium mulberry leaf ultra micro dry powder comprises the following steps:
(1), plantation is chosen and is rich in leaf protein, leaf is large, output is high mulberry tree breed;
(2), the edible fungi residue that will gather in the crops mushroom takes off bag through machinery and smashs to pieces, then adds the little fertilizer of selenium or selenium mineral powder, bacillus subtilis live bacteria agent, the bacterium slag of every 100 kilograms adds the little fertilizer of selenium or selenium mineral powder 0.5-2 kilogram; Bacillus subtilis live bacteria agent 0.5-3 kilogram mixes, and builds heap, normal temperature fermentation 15-20 days, namely gets and contains the selenium edible fungus dreg biological organic fertilizer;
(3), the process of plantation mulberry tree use prepare contain the selenium edible fungus dreg biological organic fertilizer, making selenium be enriched to mulberry leaf by bio-transformation becomes rich selenium mulberry leaf;
(4), the rich selenium mulberry leaf of results, in time carry out low-temperature vacuum drying and obtain dried mulberry leaf, then dried mulberry leaf are pulverized with micronizer, cross the 500-1000 mesh sieve, obtain to contain the feeding rich selenium mulberry leaf ultra micro dry powder of various bioactivators;
The dried mulberry leaf of selenium content 5-20 mg/kg in described rich selenium mulberry leaf.
2. the production method of rich selenium Ultra-fine Powder of Mulberry Leaves feed according to claim 1, it is characterized in that: the temperature of described low-temperature vacuum drying is 35-50 ℃.
3. the production method of rich selenium Ultra-fine Powder of Mulberry Leaves feed according to claim 1, it is characterized in that: mulberry tree is used the method that contains the selenium edible fungus dreg biological organic fertilizer, to cut down in the annual summer to adopt root to execute after cutting down with the winter, summer is cut down 0.2 kilogram of rear every strain mulberry consumption, or 1000 kilograms of every mu of consumptions, winter is cut down 0.25 kilogram of rear every strain mulberry consumption, or 1250 kilograms of every mu of consumptions.
4. the resulting rich selenium Ultra-fine Powder of Mulberry Leaves feed product of the production method of rich selenium Ultra-fine Powder of Mulberry Leaves feed claimed in claim 1.
CN 201210154600 2012-05-18 2012-05-18 Selenium-rich mulberry leaf super micropowder feed and production method thereof Active CN102640846B (en)

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CN102888376B (en) * 2012-10-12 2014-07-09 恩施和诺生物工程有限责任公司 Bacillus subtilis BC-198, and selenium-rich microbial inoculum and application thereof
CN103844032A (en) * 2012-12-04 2014-06-11 文柳璎 Method for feeding black guinea pig by taking folium mori as main feed
CN103548462B (en) * 2013-10-23 2015-07-29 南宁云涓生态科技有限公司 A kind of rich selenium gynostemma pentaphylla vegetable and production method thereof
CN104605167A (en) * 2013-11-04 2015-05-13 浙江金华神农农业科技有限公司 Feed fermentation agent and fermented feed
CN103798544A (en) * 2014-02-19 2014-05-21 丹阳市云阳镇田园圣树专业合作社 Herbal laying hen feed containing mulberry branches and leaves
CN104261916A (en) * 2014-09-22 2015-01-07 南京财经大学 Preparation method of selenium-enriched biological fertilizer and product thereof
CN105360685A (en) * 2015-11-27 2016-03-02 姚淑萍 Selenium-enriched goose feed and preparation method thereof
CN106135130A (en) * 2016-07-20 2016-11-23 重庆禾汇农业发展集团有限公司 A kind of production method of low cholesterol selenium-rich Countryside Egg
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