CN102631503A - Antifatigue traditional Chinese medicine composition and preparation method thereof - Google Patents
Antifatigue traditional Chinese medicine composition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a new application and a preparation method of a traditional Chinese medicine composition with significant treatment effect on fatigue symptoms. The medicinal preparation is a composition obtained by mixing gen-seng, poria cocos, polygala, and grass-leaved sweetflag rhizome kernel according to a certain proportion, or by active part extraction (ie, ginsenoside, pachymaran, grass-leaved sweetflag rhizome volatile components/nonvolatile components, tenuifolin or/and oligosaccharide esters), and can be prepared into various dosage forms. The traditional Chinese medicine composition is applicable to fatigue symptoms caused by various reasons. The mechanism is that the composition can recover the balance between central excitability and inhibition through regulation of central neurotransmitters and enhancement of the immune system, and thus realize the effect of exercise-induced fatigue resistance and the improvement of central fatigue states.
Description
Technical field
The invention belongs to the field of Chinese medicines; A kind of Chinese medicine composition of being made up of Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae with obvious anti-fatigue effect mainly is provided; And by Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae through extract, concentrate, refining each active constituents of medicine that obtains, promptly ginsenoside, pachyman, Rhizoma Acori Graminei volatile ingredient/involatile constituent, polygalic acid are or/and the compositions that the oligosaccharide ester is formed.Be specifically related to them in the application of the fatigue symptom that causes of treatment a variety of causes and the application of preparation anti-fatigue product thereof.Said composition exhausts maincenter 5-HT due to the motion through restraint and raises and recover the balance of central excitation property and inhibition, thereby reaches the resisting kinetic fatigue effect, especially can better improve the state that the maincenter protectiveness suppresses under the sports fatigue.
Technical background
Along with the fierceness of social competition and the quickening of rhythm of life in recent years, the tired maximum hidden danger of 21 century human health that become is that daily life and work are had a strong impact on.Tired; Be meant the sense that the whole body is weak, tired and Mental fatigue power exhausts that the decline owing to the health and the mental status causes; It is the biochemical combined reaction that changes of body complex physical; It is not normal to cause body nerve, endocrine, all systems of immunity to regulate, and in motherland's medical science, belongs to the category of the deficiency of vital energy, deficient, overstrain.
Radix Ginseng has microcirculation improvement, improve organize anti-anoxia ability, resisting fatigue, antitumor, defying age, radioprotective, etc. multiple biological activity.Radix Polygalae is rich in chemical compounds such as saponins, oligosaccharide esters, has many-sided biological activitys such as Fructus Alpiniae Oxyphyllae, tranquilizing soporific, antidepressant, oxidation-resisting and caducity, antibacterial anti-inflammatory.The Poria main chemical compositions is polysaccharide and triterpenes components, has effects such as the tumor of inhibition, antiinflammatory, adjusting immunity.The arduous temperature of Rhizoma Acori Graminei nature and flavor is gone into the heart, stomach warp, the gas of tool fragrance, and the power that row looses is stronger, is to declare the sensible good merchantable brand of gas, and function is eliminated phlegm for resuscitation, removing dampness to restore normal function of the stomach, refreshment Fructus Alpiniae Oxyphyllae, is that fragrance is allayed excitement, the key medicine of waking up the patient from unconsciousness by clearing away phlegm.To sum up, this prescription ability enhancing human body immunity function, the adaptive capacity of raising body can infer that thus it has anti-fatigue active.
Summary of the invention
Compositions and the active component combination thereof that the present invention discloses Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae first is used to treat the doctor trained in Western medicine category is the main disease and the slack property disease of traditional Chinese medical science category with the fatigue symptom.
The method for preparing of this anti-fatigue medicament is the Radix Ginseng section, doubly measures with 60% ethanol 5-10, and heating and refluxing extraction 3 times, each 0.5-2h, merge extractive liquid, filters, and filtrate recycling ethanol is subsequent use; Medicine residues of Radix Ginseng is subsequent use; Rhizoma Acori Graminei adds water 6-10 doubly, extracts volatile oil 6-10h, and volatile oil is subsequent use; Rhizoma Acori Graminei medicinal residues and water liquid, medicine residues of Radix Ginseng, Poria, Radix Polygalae are fried in shallow oil altogether, and amount of water 8-10 doubly extracts 2 times; Each 0.5-2h merges medicinal liquid, and being concentrated into relative density is 1.10～1.15 (50 ℃ of surveys); Add ethanol and make medicinal liquid contain alcohol amount to reach 50%, cold preservation (5～10 ℃) is spent the night, and filters.Reclaim ethanol, add the Radix Ginseng extractive solution, being concentrated into relative density is 1.32～1.35 (50 ℃ of surveys), and drying under reduced pressure gets medicated powder.In proportion volatile oil and medicated powder are mixed with medicinal liquid during use.Can be according to the preparation process of routine; Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae medical material or extract are pulverized the back add excipient substances such as conventional excipient, flavoring agent, disintegrating agent, antiseptic, lubricant, wetting agent, binding agent, solvent, thickening agent, solubilizing agent; Process any peroral dosage form that is suitable for using clinically, like tablet, capsule, granule, oral liquid etc.
Can Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae be obtained each active constituents of medicine through extracting, concentrate, making with extra care, promptly ginsenoside, pachyman, Rhizoma Acori Graminei volatile ingredient, involatile constituent, polygalic acid are or/and polysaccharide is processed above-mentioned any dosage form after forming compositions.
Description of drawings
Fig. 1 Chinese prescription exhausts the influence of motion number of shocks to sports fatigue mice power, with quiet matched group relatively,
#Compare with solvent control group P＜0.05,
*P＜0.05,
*P＜0.01.
Fig. 2 Chinese prescription compares with solvent control group the influence of sports fatigue mice maincenter 5-HT content,
*P＜0.05,
*P＜0.01; Compare with quiet matched group,
#P＜0.05
Fig. 3 Chinese prescription exhausts the influence of time to rat treadmill power, with model group relatively,
*P＜0.05,
*P＜0.01.
The specific embodiment
To the present invention be described further through concrete instance below:
Embodiment 1 Chinese prescription resisting fatigue and resisting oxygen lack research
One, summary
On a resisting fatigue model and anoxia enduring model, Chinese prescription shows clear and definite resisting fatigue and resisting oxygen lack.On the tired model of mice runner, Chinese prescription continuous 4 all gastric infusions, its resisting fatigue effective dose is 500mg/kg and 1000mg/kg.At mice anoxia enduring model, a continuous week of Chinese prescription is irritated stomach, but Chinese prescription 500mg/kg, 1000mg/kg dose groups significant prolongation mice anoxia enduring time-to-live explain that Chinese prescription has resisting fatigue and anoxia enduring activity.
Two, test objective
Chinese prescription is a Chinese medicine compound, and this test on behavioristics's model and biochemical indicator detect on the basis that combines, estimates its resisting fatigue and anoxia enduring is active.
Three, receive the reagent thing
Title: Chinese prescription dry powder (extraction of 301 Hospital of PLA); Hide territory Rhodiola rosea capsules (Tibet Yangke Biotechnology Co., Ltd.); Modafinil (being so kind as to give) by Academy of Military Sciences of PLA.
Four, animal
Kunming mouse, the SD rat, (the PLA General Hospital Experimental Animal Center provides the secondary animal, credit number: SCXK-(army) 2007-004.
Five, data statistics is handled
Result of the test adopts corresponding statistical method to handle, and mean is relatively checked with t between two groups.
Six, test method
1. mice hypoxia endurance test
50 of male KM mices, 18～22g is divided into normal group, the large, medium and small dose groups of Chinese prescription and Radix Rhodiolae group at random.Chinese prescription dosage is pressed 1000mg/kg, 500mg/kg, 250mg/kg gastric infusion respectively, and the positive drug group is a Rhodiola rosea capsules, presses the 180mg/kg gastric infusion.Normal group is irritated stomach equivalent normal saline.
Continuous irrigation stomach 6 days.Irritated stomach on the 7th day after 2 hours, each Mus is placed the 125ml wide mouthed bottle that the 7.5g sodica calx is housed respectively, and smear the bottleneck secluding air with vaseline.Write down each Mus death time.
2. mouse anti-reflecting fatigue test
Animal divides into groups: animal divides into groups: 84 of male KM mices, 18～22g is divided into normal group, the large, medium and small dose groups of Chinese prescription, Radix Rhodiolae group, modafinil group and quiet matched group at random.Chinese prescription dosage is pressed 1000mg/kg, 500mg/kg, 250mg/kg gastric infusion respectively, and Rhodiola rosea capsules is pressed 180mg/kg, modafinil is pressed the 13mg/kg gastric infusion, and normal group and quiet matched group are irritated stomach equivalent distilled water.After irritating stomach 1h every day, remove quiet matched group, each Mus is trained on rotary-type tired appearance; Continued for 4 weeks, 5 days weekly, had a rest 2 days; Rotating speed weekly is respectively 10m/min, 12m/min, 13m/min and 15m/min, and movement time is respectively 30min, 30min, 60min and 60min..The power of carrying out the 17m/min time after 4 weeks and be 60min exhausts motion (except the quiet matched group), writes down each Mus power and exhausts the motion number of shocks, and power is got blood, full brain, liver, femoribus internus muscle test kit to be done with each Mus rapidly after exhausting motion.
After mice is put to death; With continuing 1 minute in the ethanol of its immersion 75%, under aseptic condition, take out spleen afterwards immediately immediately, in the plate of 4 ℃ of Hank ' s liquid, clean one time; The back is pulverized tissue on the net at 200 order stainless steel silks with the syringe nook closing member see through in silk screen entering Hank ' the s liquid individual cells; The centrifugal 5min of 1000rpm abandons supernatant, adds 5ml again and contains 5%NH
4Among the TrisHCl of Cl, leave standstill 10min and make EF, the centrifugal 10min of 1000rpm abandons supernatant once more, adds to contain in the RPMI RPMI-1640 of 10% hyclone, makes cell density reach 2*10
6/ L is divided into normal group and ConA stimulating group.Get 96 well culture plates, respectively organize every hole normally and add 90 μ L chrotoplast suspensions, the blank culture medium of 10 μ L, 8 every group multiple holes.Every hole of respectively organizing that ConA stimulates adds the 90ul splenocyte suspension; ConA (making final concentration is 10 μ g/mL) 10 μ L, 8 every group multiple holes.Put 37 ℃, cultivate 72h in the 5%CO2 incubator.Mtt assay detects splenocyte propagation and gets the splenocyte of cultivating 72h, and every hole adds 20 μ L MTT, continues to hatch 4h, and careful suction adds DMSO 150 μ L after abandoning supernatant, and 37 ℃ of vibration 30min survey the OD value in the 570nm place.Adopt the double-antibody sandwich elisa method to measure IL-2 and IL-4 content in the cell conditioned medium liquid, experimental result is read the OD value at ELIASA 450nm place.
3. rat anti fatigue test
Animal divides into groups: 42 of male SD rats, 180～220g is divided into normal group, the large, medium and small dose groups of Chinese prescription, Radix Rhodiolae group, modafinil group and quiet matched group, 6 every group at random.Chinese prescription dosage is pressed 1000mg/kg, 500mg/kg, 250mg/kg gastric infusion respectively, and Rhodiola rosea capsules is pressed 180mg/kg, modafinil is pressed the 13mg/kg gastric infusion, and solvent control group and quiet matched group are irritated stomach equivalent distilled water.
After irritating stomach 1h every day, each Mus is trained on the rat treadmill, continued for 5 weeks, 6 days weekly.Except that quiet matched group, each group rat is trained 20min every day on treadmill, 6 days weekly, the gradient was 0, running velocity increases progressively weekly; Be followed successively by 15,22,25,31; 35m/min, totally 5 weeks. carrying out the gradient subsequently is 0, and speed is the treadmill training of 35m/min, exhausts until power.It is that animal does not catch up with predetermined running velocity that power exhausts criterion, and the buttocks recoil is in the treadmill rear wall, and for 30s, that acousto-optic electricity and hairbrush stimulate hind leg is invalid with the rotating belt holder, rapid breathing, look tired is, prostrate hangs one's head, to stimulating not reaction.Power exhausts the motion back all experimental rat eye sockets is got blood, full brain, test kit to be done.
Seven, result of the test
1. mice hypoxia endurance test
Chinese prescription continuous irrigation stomach is after 7 days, and Chinese prescription is at 500mg/kg and 1000mg/kg dosage, and the time-to-live obviously prolongs (P＜0.01), sees table 1.Wherein the 1000mg/kg dose groups anoxia mouse brain tissue SOD vigor that can raise reduces MDA concentration.This test explanation Chinese prescription has tangible anoxia enduring active on this model.
Table 1 Chinese prescription to the influence of mice normal pressure anoxia enduring time-to-live and cerebral tissue SOD vigor, MDA concentration (x ± s, n=10)
Annotate: compare with matched group,
*P＜0.05,
*P＜0.01.
2. mouse anti-reflecting fatigue test
Chinese prescription on 250mg/kg, 500mg/kg and 1000mg/kg dosage to mice power exhaust when motion number of shocks compare the model group group minimizing trend all arranged, significantly minimizing power exhausts motion number of shocks (see figure 1) on 500mg/kg, 1000mg/kg dosage.
The quieter matched group of solvent control group mice maincenter 5-HT significantly raise (P＜0.05); Chinese prescription is compared with solvent control group the influence of 5-HT content in the sports fatigue mouse brain tissue on 1000mg/kg dosage all has significant difference (P＜0.01), sees Fig. 2.
In the table 2, solvent control group Mouse Liver glycogen, muscle glycogen content are compared with quiet matched group all and are significantly reduced, and the quieter matched group of lactic acid concn significantly raises in the muscle.Chinese prescription relative solvent control group of synthetic influence to sports fatigue Mouse Liver glycogen on 500mg/kg, 1000mg/kg dosage significantly increases (P＜0.05), and the decomposition of the muscle glycogen that on 500mg/kg dosage, slows down simultaneously is than the remarkable increase of solvent control group (P＜0.05).This medicine on 500mg/kg, 1000mg/kg dosage to the sports fatigue mouse muscle in lactic acid concn compared significantly reduced trend with solvent control group.See table 2.
Table 2 Chinese prescription to the influence of lactic acid concn in motion Mouse Liver glycogen content, muscle glycogen content and the muscle (.x ± s, n=12)
Annotate: compare with solvent control group,
*P＜0.05,
*P＜0.01. and quiet matched group compare,
#P＜0.05,
##P＜0.01.
Can be known that by table 3 solvent control group mice serum LDH vigor, blood serum urea nitrogen concentration, the quieter matched group of serum MDA concentration all significantly raise (P＜0.01), serum activity of SOD, the quieter matched group of serum testosterone concentration significantly reduce (P＜0.01).Chinese prescription on 500mg/kg, 1000mg/kg dosage to the sports fatigue mice serum in the LDH vigor compare reduction with solvent control group, and significant difference (P＜0.01) is arranged.Chinese prescription has the effect of reduction to the concentration of blood urea nitrogen in the sports fatigue mice serum, has compared significant difference (P＜0.05) with solvent control group at 250mg/kg, 500mg/kg, and difference reaches maximum (P＜0.01) on 1000mg/kg dosage, sees table 3.Chinese prescription is compared all significantly rising (P＜0.01) to the serum testosterone content of mice with solvent control group on three dosage, see table 3.In addition, Chinese prescription can significantly improve sports fatigue mice serum SOD vigor on 500mg/kg, 1000mg/kg dosage, reduces serum MDA concentration.
Table 3 Chinese prescription to the influence of motion mice serum lactic acid dehydrogenase (LDH) vigor, urea nitrogen concentration, testosterone concentration, SOD vigor and MDA concentration (.x ± s, n=12)
Annotate: compare with solvent control group,
*P＜0.05,
*P＜0.01.Compare with quiet matched group,
#P＜0.05,
##P＜0.01.
Can find out that from table 4 with quiet matched group group ratio, normal splenocyte of solvent control group and the splenocyte vigor that stimulates through ConA obviously reduce.With the model group ratio, three dose groups of Chinese prescription all can significantly promote normal splenocyte and the splenocyte propagation that stimulates through ConA.The normal splenocyte of solvent control group and all stimulate without ConA and significantly raise (P＜0.01) through the quiet matched group IL-2 of ConA stimulation through the splenocyte that ConA stimulates, IL-4 significantly reduces (P＜0.01).Chinese prescription 500mg/kg, 1000mg/kg all can significantly reduce normal splenocyte and the secretion of the splenocyte IL-2 that stimulates through ConA and increase normal splenocyte and the secretion of the splenocyte IL-4 that stimulates through ConA.
Table 4 Chinese prescription to the influence of motion mouse boosting cell vigor and splenocyte supernatant IL-2, IL-4 concentration and the two ratio (.x ± s, n=12)
Annotate: compare with solvent control group,
*P＜0.05,
*P＜0.01.Compare with quiet matched group,
#P＜0.05,
##P＜0.01.
3. rat anti fatigue test
Can know that by Fig. 3 Chinese prescription can significant prolongation rat power exhaust movement time at 500mg/kg and 1000mg/kg dosage.
In table 5, beta-endorphin content is compared remarkable rising (P＜0.01) in the solvent control group rat cerebral tissue with quiet matched group.Chinese prescription can significantly reduce the concentration of sports fatigue rat maincenter beta-endorphin on 500mg/kg, 1000mg/kg dosage.The concentration of testosterone is compared remarkable reduction (P＜0.01) in the solvent control group rat blood serum with quiet matched group.On three dosage, can significantly the raise concentration of sports fatigue rat blood serum testosterone of Chinese prescription.
Table 5 Chinese prescription is to (the influence of β-EP) and serum testosterone content of treadmill exercise rat maincenter beta-endorphin.
Annotate:
*Compare with solvent control group P＜0.01,
#P＜0.05,
##Compare with quiet matched group group P＜0.01.
As shown in table 6, to compare with quiet matched group, solvent control group rat blood serum Trp, maincenter Trp, maincenter 5-HT and maincenter 5-HIAA content is significantly rising (P＜0.01) all.Chinese prescription 500mg/kg, 1000mg/kg dose groups all can significantly reduce treadmill exercise rat blood serum Trp and maincenter Trp content.Chinese prescription 1000mg/kg dose groups can significantly reduce treadmill exercise rat maincenter 5-HT and maincenter 5-HIAA content.
Table 6 Chinese prescription is to the influence of treadmill exercise rat blood serum tryptophan (Trp), maincenter Trp, middle serotonin (5-HT) and maincenter pentahydroxy-heteroauxing (5-HIAA) content
Annotate:
*P＜0.05,
*Compare with solvent control group P＜0.01,
##Compare with quiet matched group group P＜0.01.
Eight, discuss
Anoxia can make the SOD vigor reduce and MDA content raises, and prompting free-radical generating in this situation lower body increases, and has increased the weight of the injury of body like this.Mainly be because the free radical very strong material that is a kind of activity, can the oxidation cell membrane on unsaturated fatty acid form MDA, thereby oxidation resistance is descended the SOD vigor is descended, so under the high condition of hypoxia, possibly increase the weight of cell injury.This experimental result shows that Chinese drug-treated group can prolong the mice anoxia enduring time-to-live, strengthens in the anoxia mouse brain tissue SOD vigor and reduces MDA concentration, has alleviated that the oxidative stress in the mouse brain tissue damages under the anoxia condition, and presents dose dependent.
Tired model of runner and rat treadmill model are in recent years comparatively general anti-fatigue test methods.The number of shocks of solvent control group mice obviously more than each group of Chinese prescription, explains that Chinese prescription demonstrates the obvious anti-fatigue effect on Animal Behavior Science.Exhaust in the motion in rat treadmill power, Chinese prescription 500mg/kg and the 1000mg/kg dose groups power time that exhausts obviously are longer than solvent control group, explain that Chinese drug-treated group can obviously improve the endurance of treadmill exercise rat.
5-HT is the important neurotransmitter of body, participates in the nervous pathway of brain, controls multiple brain function, and can influence body motivation ability.When the body prolonged exercise; The Trp that sees through in the blood brain barrier entering brain increases; By the neurocyte picked-up, generate 5-HT through hydroxylation and decarboxylic reaction, the increase of the 5-HT in the brain causes cerebral cortex is suppressed to strengthen; Excite symptoms such as sleepiness, indolent and listless, inappetence, impel central fatigue to produce.5-HIAA is the catabolic end product of 5-HT.In the experiment of mice runner; Solvent control group is after modeling and the power of last day in 4 weeks of experience exhaust motion; 5-HT concentration is significantly higher than quiet matched group in the maincenter, and Chinese prescription is respectively organized dosage and all the trend of reduction arranged and be dose dependent at the 5-HT to excessive rising.In the rat tread mils test; Chinese prescription is respectively organized dosage and is all being reduced confirmed fatigue rat blood serum Trp due to the treadmill exercise, maincenter Trp, 5-HT and 5-HIAA content in varying degrees; Explain that this prescription possibly have the effect that is adjusted in transmitter in central nervous system abnormal secretion under the stress state; The unusual rising of maincenter 5-HT that promptly exhausts due to the motion through restraint recovered the balance of central excitation property and inhibition; Thereby reach the resisting kinetic fatigue effect, especially can better improve the state that the maincenter protectiveness suppresses under the sports fatigue.
When the body prolonged exercise, consume muscle glycogen and hepatic glycogen, keep normal blood sugar level.Behind glycogen depletion, blood sugar concentration reduces, and central nervous system's energy supply is unfavorable and occur tiredly, and the accumulation of lactic acid in the body simultaneously influences contractile protein to Ca
2+Sensitivity, the contraction strength that causes contractile protein to produce weakens, and brings out the fatigue of skeletal muscle, therefore can influence the relatively stable and intravital homergy process of organismic internal environment.Hepatic glycogen and muscle glycogen deposit are more sufficient, and it provides the required energy of motion just many more, and fatigue will postpone to occur.In the experiment of mice runner, Chinese prescription 500mg/kg, 1000mg/kg dose groups can reduce concentration of lactic acid in the muscle through the runner motion mice muscle glycogen regulating liver-QI glycogenolysis that slows down, and have alleviated lactic acid and have piled up the muscle fatigue that causes.Thereby, body reaches the antifatigue purpose for providing more energy.
Biological oxidation is strengthened in the high-intensity exercise; Make skeletal muscle be in anaerobic condition; Also making wherein, acetone acid increases; The active enhancing of LDH helps again changing acetone acid into lactic acid, so the LDH activity also is to estimate one of tired important biochemical indicator, its rising has shown the high-level state of lactic acid and acetone acid in the metabolic process; Strenuous exercise causes organism fatigue and can cause the energy balance to be destroyed, and the catabolism of protein and nitrogen-containing compound is strengthened, and the formation that is accompanied by carbamide increases.The body urea nitrogen content increases with the increase of work or sports load, and body is poor more to load performance, and blood urea nitrogen increases just obvious more; Testosterone is also closely related with motor capacity; Mainly showing stimulates that the body internal protein is synthetic, the generation that increases erythropoietin, promotes bone marrow hematogenesis, the overrecovery of the back muscle glycogen that moves is played the part regulating action reduces the UCre amount, makes creatine and ATP effect synthesize the growth of phosphagen, promotion bone and cortical bone is thickened etc.The tired in addition increase that also can cause free radical in the body, malonaldehyde (MDA) is the important metabolite of body lipid peroxidation, is used for representing free-radical generating amount and cell membrane lipid peroxidating degree, indirect reaction cell injury degree.In the experiment of mice runner, observe, Chinese drug-treated group can significantly reduce sports fatigue mice serum blood urea nitrogen, MDA concentration and LDH vigor, and improves the SOD vigor, thereby reaches fatigue effect.
Proper training intensity and quantity of motion can improve body's immunological function; But hyperkinesia; When especially moving fatigue, the immunity of body is reduced, the susceptibility that shows as infectious disease increases; The immunocyte of body and some immune factor change, thereby motor capacity is descended.The spleen lymphocyte proliferation ability is the important indicator of body cell immunologic function, and the amount that IL-2 and IL-4 produce and the proliferation and differentiation of T cell and B cell, with the killing activity of mononuclear phagocyte, NK cell and LAK cell substantial connection is arranged.This result shows that behind the strenuous exercise, the mice spleen lymphocytes proliferation ability significantly reduces; Remarkable rising of IL-2 content and IL-4 content significantly reduce in the splenocyte supernatant, and after the Chinese prescription treatment, sports fatigue mouse boosting cell vigor obviously strengthens; IL-2 and IL-4 content are near quiet matched group; Point out this medicine can promote mice spleen lymphocytes proliferation, regulate the abnormal secretion of IL-2 and IL-4, regulate and improve body's immunity, thereby reach the effect of alleviating sports fatigue.
Nine, experiment conclusion
Can find out that through above analysis Chinese prescription has tangible resisting oxygen lack and aspect generation that improves the adaptive capacity of body to sports load, delay fatigue and the elimination that accelerates fatigue effect clearly arranged all.
Claims (5)
1. the Chinese medicine composition application in preparation antifatigue medicine, its Chinese medicine composition are to be 3-9: 3-6: 2-10 by Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae proportioning: 2-8 processes.
2. according to claim 1 and the described Chinese medicine composition of claim 2; Be meant that Radix Ginseng, Poria, Rhizoma Acori Graminei, Radix Polygalae obtain each active constituents of medicine through extracting, concentrate, making with extra care, promptly ginsenoside, pachyman, Rhizoma Acori Graminei volatile ingredient/involatile constituent, polygalic acid are or/and the compositions that the oligosaccharide ester is formed.
3. method for preparing with anti-fatigue medicament is characterized in that: the Radix Ginseng section, doubly measure with 60% ethanol 5-10, and heating and refluxing extraction 3 times, each 0.5-2h, merge extractive liquid,, filtration, filtrate recycling ethanol, subsequent use; Medicine residues of Radix Ginseng is subsequent use; Rhizoma Acori Graminei part adds water 6-10 doubly, extracts volatile oil 6-10h, and volatile oil is subsequent use; Rhizoma Acori Graminei medicinal residues and water liquid, medicine residues of Radix Ginseng, Poria, Radix Polygalae are fried in shallow oil altogether, and amount of water 8-10 doubly extracts 2 times; Each 0.5-2h merges medicinal liquid, and being concentrated into relative density is 1.10～1.15 (50 ℃ of surveys); Add ethanol and make medicinal liquid contain alcohol amount to reach 50%, cold preservation (5～10 ℃) is spent the night, and filters.Reclaim ethanol, add the Radix Ginseng extractive solution, being concentrated into relative density is 1.32～1.35 (50 ℃ of surveys), and drying under reduced pressure gets medicated powder.In proportion volatile oil and medicated powder are mixed with medicinal liquid during use.
4. according to claim 1 and the described Chinese medicine composition of claim 2; Be meant excipient substances such as adding conventional excipient, flavoring agent, disintegrating agent, antiseptic, lubricant, wetting agent, binding agent, solvent, thickening agent, solubilizing agent; Process any dosage form that is suitable for using clinically, like tablet, capsule, granule, oral liquid, injection etc.
5. application according to claim 1, wherein the fatigue symptom of indication is meant because the fatigue symptom that a variety of causes causes (the slack property disease that comprises periphery fatigue, central fatigue and traditional Chinese medical science category).
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766881A (en) * | 2012-10-26 | 2014-05-07 | 苏州市洋海电子有限公司 | Traditional Chinese medicine health product |
| CN110623969A (en) * | 2019-09-05 | 2019-12-31 | 河北中医学院 | Component formula for treating amnesia |
| CN111393534A (en) * | 2020-02-21 | 2020-07-10 | 广东药科大学 | Acorus gramineus sugar polymer and preparation method and application thereof |
| CN111393534B (en) * | 2020-02-21 | 2022-04-22 | 广东药科大学 | Acorus gramineus sugar polymer and preparation method and application thereof |
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