CN102628841B - Parabolic high-performance liquid chromatographic preparative column - Google Patents
Parabolic high-performance liquid chromatographic preparative column Download PDFInfo
- Publication number
- CN102628841B CN102628841B CN201110071563.6A CN201110071563A CN102628841B CN 102628841 B CN102628841 B CN 102628841B CN 201110071563 A CN201110071563 A CN 201110071563A CN 102628841 B CN102628841 B CN 102628841B
- Authority
- CN
- China
- Prior art keywords
- parabola shaped
- column
- tubular cylinder
- shaped tubular
- cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The invention relates to a parabolic high-performance liquid chromatographic preparative column. The capillary liquid chromatographic preparative column comprises a column cap positioned on the upper portion of a column body, the parabolic tubular column body and a column end positioned on the lower portion of the column body, wherein an inlet and an outlet of the parabolic tubular column body are respectively provided with a special seal plunging member, the column body is designed into a parabolic shape and parabolic equation is corresponding to flow velocity. Therefore, a perfect plug-shaped chromatographic band can be obtained and column efficiency of the chromatographic column is greatly raised. The internal diameter of the column body is gradually decreased, and the sample loading amount of the chromatographic column is increased, thus leading to a certain enrichment effect of the sample and greatly reducing the preparation cost.
Description
Technical field
The present invention relates to a kind of performance liquid chromatographic column, particularly relate to a kind of parabola shaped high-performance liquid chromatogram preparation column.
Background technology
Chromatography, since Tswett in 1906 foundes, has all obtained tremendous development aspect analysis and preparation two, and has been widely used in the every field in productive life.In the separation to secondary class metabolic product and natural products, the aspects such as macromolecular separation and purification, preparative chromatography is all being served as key player.Simultaneously chromatographic resolution is also separation with the preparation of diagnostic reagent for the important medicinal organism activated protein of some high-purity, biological therapy product.Along with scientific and technical development, purity and the output requirement of people to various reagent, medicine is also more and more higher, and for satisfying the demands, people are also more and more in the research of new packing and new column packing technique.Particularly, in preparative chromatography column technology field, how to improve post effect and the selectivity of preparative liquid chromatography, reduce production costs, obtaining high-purity sample becomes the focus of research.
In preparative chromatography separates, people once considered a wide internal diameter post narrow internal diameter post long with to connect, and because wide internal diameter post above has larger capacity, can avoid pillar to transship, and narrow internal diameter post below can provide effective separation.Nineteen sixty-eight, [the Joel Kwok such as Joel Kwok, L R.Snyder, J.C.Sternberg.Anal.Chem., 1968,40 (1): 118-122] studied the problem that different inner diameters post is connected, find that series connection is not the good method that improves post effect, because the plate height of wide internal diameter post in columns in series is above very large, narrow internal diameter post below can be ignored in contrast sometimes, therefore can not effectively play the effect that improves post effect.But, 1981, Said[A.S.Said.Journal of High Resolution Chromatography & Chromatography Communications, 1979, Febuary:63-67] by theory, calculate tapered pole [100 (column length) × 3 (entrance internal diameter) → 1 (outlet internal diameter), unit: cm, lower same] mutually more identical in quality than fixing, series connection cylindrical pillars [41.7+58.3 (buttress shaft length) × 1 (buttress shaft internal diameter), (thick column length) × 3 (thick column internal diameter) that total length is identical, unit: cm] there is higher efficiency, tapered pole post effect is almost 2 times of columns in series post effects, the tapered pole therefore with the even transition of wide portion internal diameter is hopeful for the preparation of chromatogram.
1966, [H.M.Stahr, R.M.Ikeda, the E.T.Oakley such as the H.M.Stahr in U.S. Philip Marris research centre, B.M.Carter.Anal.Chem., 1966,38:19741 makes low pressure taper liquid chromatography post first, and they adopt vinyon as column material, silica gel is fixing phase, homogenate method is filled, and sample is azobenzene, and eluant, eluent is normal hexane.For investigating the feature of tapered pole, having made in addition two has the chromatographic column of same volume to compare with tapered pole, and one is conventional cylindrical glass post, another three rank cylindrical glass posts that are internal diameter varies.Relatively the bands of a spectrum volume of three pillars is to weigh column efficiency, and result shows that tapered pole is compared with three rank cylindrical pillars, and post effect is basically identical, but tapered pole post effect is obviously better than single order cylindrical pillars, and its bands of a spectrum volume is the half of single order cylindrical pillars.Nineteen ninety, the Moerker Theophile of Switzerland has invented a kind of entrance internal diameter and has been less than the tapered pole [Switzerland Patent CH675212,1990] that exports internal diameter.Can be gradually when sample flows in post to tube wall diffusion, in the tapered pole expanding gradually at this internal diameter, because column type has met the trend of sample diffusion, solute band can not be diffused into tube wall, thereby presents " infinite diameter effect ".The great advantage of this tapered pole is that post effect is high, but because entrance internal diameter is little, thereby the ability of load sample is less than same volume, the column type post with length.In addition, about the research of taper liquid chromatography analytical column also has some reports, as tapered pole (150 × 4 → 2.5mm, 0.6 ° of cone angle) [A.Pecavar, the I.Vovk of Slovenia country chemistry institute report, J.Marsel, M.Prosek.Analytical Sciences.1999,15:233-240], the tapered pole (25mm × 2 → 0.5mm of the Nugent Kerry D. invention of the U.S., 3.4 ° of cone angles) [World PatentWO00/74808,2000].
For overcoming " wall effect " of chromatographic column, improve post effect, the Moerker Theophile of Switzerland has invented the large tapered pole of a kind of column cap pillar tail, because cylindricality has met the trend spreading to tube wall gradually when sample flows in post, solute band can not be diffused into tube wall, present " infinite diameter effect ", greatly improved post effect; But this chromatographic column column cap is little, has greatly limited sample size.2005, Guan Yafeng etc. invented the diminishing tapered pole of a kind of internal diameter, by adjusting flow velocity, obtained to such an extent that approach stopper shape flow pattern, had improved post effect; While is because opening internal diameter is large, thereby applied sample amount increases.2009, Zhang Weibing etc. invented a kind of spherical chromatographic column, and its first spherical cylinder internal diameter increases gradually, can overcome " post jamb effect ", improved post effect; Second spherical cylinder, can obtain stopper shape flow pattern, and sample is had to enrichment effect.But so far, there is no the report about parabola shaped high-performance liquid chromatogram preparation column.
Summary of the invention
For above-mentioned background, the object of this invention is to provide one and take into account post effect and sample size, and sample is there is to the parabola shaped high-performance liquid chromatogram preparation column of certain enrichment effect.
The object of the invention is to be achieved through the following technical solutions:
A kind of parabola shaped high-performance liquid chromatogram preparation column, is characterized in that, described parabola shaped high-performance liquid chromatogram preparation column comprises: be positioned at column cap, the parabola shaped tubular cylinder on cylinder top and be positioned at the post tail of cylinder bottom; Wherein, column cap and post tail are assemblied in respectively import and the outlet of parabola shaped cylinder by flange;
Described parabola shaped tubular cylinder has that internal diameter reduces gradually and the opening shape flattened oval type inlet end of even transition, and internal diameter increases the flat rhombus endpiece of opening shape of even transition gradually, described inlet end is with the inlet tube being integrally molded with described parabola shaped tubular cylinder, described endpiece is with the outlet being integrally molded with described parabola shaped tubular cylinder, and to keep, liquid phase mobile phase first reduces equably, flow out to rear increase parabola shaped tubular cylinder;
The inlet end internal diameter 2R=100mm～150mm of described parabola shaped tubular cylinder, endpiece internal diameter 2r=30mm～60mm, column length L=85cm～95cm, the shape of parabola shaped tubular cylinder is specially: burnt accurate apart from 0.1≤p≤100, far ultraviolet post jamb is to the distance 10mm≤L≤10000mm of focus; Wherein, the parabolic equation of described parabola shaped cylinder is corresponding with different in flow rate, and wherein, Jiao of the larger correspondence of flow velocity is accurate apart from the less parabola shaped tubular cylinder of distance larger, that far ultraviolet post jamb arrives focus;
The inlet end of described parabola shaped tubular cylinder and endpiece have respectively a sealing and stretch into part, described sealing is stretched into part and is made by olefinic rubber or thermoplastic resin, sealing is stretched into part and is had the induction pipe and the two sided sealing inclined-plane that are integrally molded, described two sided sealing inclined-plane makes described sealing stretch into part to slip into endpiece and the inlet end of described parabola shaped tubular cylinder, to form uniform zero dead volume wedge type sealing; The outside surface that part is stretched in described sealing has the corrosion-resistant coating of anti-soda acid, deposits double-layer seal layer on described corrosion-resistant coating;
Described column cap comprises the sieve plate in ring flange, bolt, gathering sill, butterfly O-ring seal, distribution plate and the non-equilateral triangle of multiple isosceles hole; Column cap is connected with parabola shaped tubular cylinder inlet end with bolt with ring flange;
Described post tail comprises with the O-ring seal of annular groove, sieve plate, ring flange and bolt with multiple equilateral triangles hole; Post tail and parabola shaped tubular cylinder endpiece are with ring flange and bolt seal.
As a kind of optimal way, described distribution plate is that the polymer organic plastics punching that 0.12～0.15mm is thick becomes the disk with otch, distribution plate middle body has all dispensing orifices identical with the equilateral triangle hole of described post tail sheet plate of shape and number, and multiple adjacent fin juts, this fin jut can accurately be located described distribution plate, and controls distribution plate accurately switching in real time when closing with latched position.
As a kind of optimal way, described burnt accurate apart from 10≤p≤20.
As a kind of optimal way, described far ultraviolet post jamb is to the distance 90mm≤L≤100mm of focus.
Compared with prior art, good effect of the present invention is:
1, because the existence of parabola shaped tubular cylinder internal diameter varies, when mobile phase flows in chromatographic column, is subject to resistance to be less than its suffered resistance in post in post jamb place.From fluid mechanics, it is greater than the flow velocity in post center at post jamb district flow velocity, and chromatographic band flow pattern becomes lower parabolic shape, and this parabolic shape is relevant to flow velocity.
Cylinder of the present invention adopts parabola shaped design, and column internal diameter is reduced gradually, and the amplitude that post jamb district mobile phase linear velocity increases is greater than post center, from post jamb to post center line velocity, reduces gradually.And can provide different equations parabola shaped cylinder, under different in flow rate, use corresponding parabola shaped cylinder, thereby obtain the stopper shape chromatographic band flow pattern of almost Perfect, greatly improve chromatographic column post effect.
2, to different flow velocitys, only need to change corresponding parabola shaped cylinder, can reach whole effects, reduced column jecket processing cost.
3, entrance internal diameter is greater than outlet internal diameter, and sample charge capacity is increased; And because internal diameter reduces gradually, sample is had to certain concentration effect, therefore in preparation is produced, only need the solvent of processing still less just can obtain the same product, can reduce solvent consumption, reduce production costs.
Accompanying drawing explanation:
Fig. 1 is the parabola shaped efficient capillary liquid chromatography preparation post of capillary schematic diagram in the present invention.
Fig. 2 be in the present invention the parabola shaped efficient capillary liquid chromatography preparation post of capillary to theaflavin preparative chromatography figure.
Wherein: 1, column cap, 2, cylinder, 3, post tail, 4, ring flange, 5, distribution plate, 6, sieve plate, 7, bolt, 8, O-ring seal.
Embodiment:
Use this parabola shaped high-performance liquid chromatogram preparation column, separate theaflavin, mobile phase is: 20% acetonitrile, 1% ethyl acetate, 79% water (including 1% acetic acid); Flow velocity: 25mL/min; Detect wavelength: 280nm.Separating resulting is shown in Fig. 2, and four kinds of kinds of theaflavin monomer are separated (referring to Fig. 2) completely.
Embodiment 1
A kind of parabola shaped high-performance liquid chromatogram preparation column, comprising: be positioned at column cap, the parabola shaped tubular cylinder on cylinder top and be positioned at the post tail of cylinder bottom; Wherein, column cap and post tail are assemblied in respectively import and the outlet of parabola shaped cylinder by flange;
Described parabola shaped tubular cylinder has that internal diameter reduces gradually and the opening shape flattened oval type inlet end of even transition, and internal diameter increases the flat rhombus endpiece of opening shape of even transition gradually, described inlet end is with the inlet tube being integrally molded with described parabola shaped tubular cylinder, described endpiece is with the outlet being integrally molded with described parabola shaped tubular cylinder, and to keep, liquid phase mobile phase first reduces equably, flow out to rear increase parabola shaped tubular cylinder;
The inlet end internal diameter 2R=100mm～150mm of described parabola shaped tubular cylinder, endpiece internal diameter 2r=30mm～60mm, column length L=85cm～95cm, the shape of parabola shaped tubular cylinder is specially: burnt accurate apart from 0.1≤p≤100, far ultraviolet post jamb is to the distance 10mm≤L≤10000mm of focus; Wherein, the parabolic equation of described parabola shaped cylinder is corresponding with different in flow rate, and wherein, Jiao of the larger correspondence of flow velocity is accurate apart from the less parabola shaped tubular cylinder of distance larger, that far ultraviolet post jamb arrives focus;
The inlet end of described parabola shaped tubular cylinder and endpiece have respectively a sealing and stretch into part, described sealing is stretched into part and is made by olefinic rubber or thermoplastic resin, sealing is stretched into part and is had the induction pipe and the two sided sealing inclined-plane that are integrally molded, described two sided sealing inclined-plane makes described sealing stretch into part to slip into endpiece and the inlet end of described parabola shaped tubular cylinder, to form uniform zero dead volume wedge type sealing; The outside surface that part is stretched in described sealing has the corrosion-resistant coating of anti-soda acid, deposits double-layer seal layer on described corrosion-resistant coating;
Described column cap comprises the sieve plate in ring flange, bolt, gathering sill, butterfly O-ring seal, distribution plate and the non-equilateral triangle of multiple isosceles hole; Column cap is connected with parabola shaped tubular cylinder inlet end with bolt with ring flange;
Described post tail comprises with the O-ring seal of annular groove, sieve plate, ring flange and bolt with multiple equilateral triangles hole; Post tail and parabola shaped tubular cylinder endpiece are with ring flange and bolt seal.
Wherein, described distribution plate is that the polymer organic plastics punching that 0.12～0.15mm is thick becomes the disk with otch, distribution plate middle body has all dispensing orifices identical with the equilateral triangle hole of described post tail sheet plate of shape and number, and multiple adjacent fin juts, this fin jut can accurately be located described distribution plate, and controls distribution plate accurately switching in real time when closing with latched position.
A kind of parabola shaped high-performance liquid chromatogram preparation column, comprising: be positioned at column cap, the parabola shaped tubular cylinder on cylinder top and be positioned at the post tail of cylinder bottom; Wherein, column cap and post tail are assemblied in respectively import and the outlet of parabola shaped cylinder by flange;
Described parabola shaped tubular cylinder has that internal diameter reduces gradually and the opening shape flattened oval type inlet end of even transition, and internal diameter increases the flat rhombus endpiece of opening shape of even transition gradually, described inlet end is with the inlet tube being integrally molded with described parabola shaped tubular cylinder, described endpiece is with the outlet being integrally molded with described parabola shaped tubular cylinder, and to keep, liquid phase mobile phase first reduces equably, flow out to rear increase parabola shaped tubular cylinder;
The inlet end internal diameter 2R=100mm～150mm of described parabola shaped tubular cylinder, endpiece internal diameter 2r=30mm～60mm, column length L=85cm～95cm, the shape of parabola shaped tubular cylinder is specially: burnt accurate apart from 0.1≤p≤100, far ultraviolet post jamb is to the distance 10mm≤L≤10000mm of focus; Wherein, the parabolic equation of described parabola shaped cylinder is corresponding with different in flow rate, and wherein, Jiao of the larger correspondence of flow velocity is accurate apart from the less parabola shaped tubular cylinder of distance larger, that far ultraviolet post jamb arrives focus;
The inlet end of described parabola shaped tubular cylinder and endpiece have respectively a sealing and stretch into part, described sealing is stretched into part and is made by olefinic rubber or thermoplastic resin, sealing is stretched into part and is had the induction pipe and the two sided sealing inclined-plane that are integrally molded, described two sided sealing inclined-plane makes described sealing stretch into part to slip into endpiece and the inlet end of described parabola shaped tubular cylinder, to form uniform zero dead volume wedge type sealing; The outside surface that part is stretched in described sealing has the corrosion-resistant coating of anti-soda acid, deposits double-layer seal layer on described corrosion-resistant coating;
Described column cap comprises the sieve plate in ring flange, bolt, gathering sill, butterfly O-ring seal, distribution plate and the non-equilateral triangle of multiple isosceles hole; Column cap is connected with parabola shaped tubular cylinder inlet end with bolt with ring flange;
Described post tail comprises with the O-ring seal of annular groove, sieve plate, ring flange and bolt with multiple equilateral triangles hole; Post tail and parabola shaped tubular cylinder endpiece are with ring flange and bolt seal.
Wherein, described burnt accurate apart from 10≤p≤20.
A kind of parabola shaped high-performance liquid chromatogram preparation column, comprising: be positioned at column cap, the parabola shaped tubular cylinder on cylinder top and be positioned at the post tail of cylinder bottom; Wherein, column cap and post tail are assemblied in respectively import and the outlet of parabola shaped cylinder by flange;
Described parabola shaped tubular cylinder has that internal diameter reduces gradually and the opening shape flattened oval type inlet end of even transition, and internal diameter increases the flat rhombus endpiece of opening shape of even transition gradually, described inlet end is with the inlet tube being integrally molded with described parabola shaped tubular cylinder, described endpiece is with the outlet being integrally molded with described parabola shaped tubular cylinder, and to keep, liquid phase mobile phase first reduces equably, flow out to rear increase parabola shaped tubular cylinder;
The inlet end internal diameter 2R=100mm～150mm of described parabola shaped tubular cylinder, endpiece internal diameter 2r=30mm～60mm, column length L=85cm～95cm, the shape of parabola shaped tubular cylinder is specially: burnt accurate apart from 0.1≤p≤100, far ultraviolet post jamb is to the distance 10mm≤L≤10000mm of focus; Wherein, the parabolic equation of described parabola shaped cylinder is corresponding with different in flow rate, and wherein, Jiao of the larger correspondence of flow velocity is accurate apart from the less parabola shaped tubular cylinder of distance larger, that far ultraviolet post jamb arrives focus;
The inlet end of described parabola shaped tubular cylinder and endpiece have respectively a sealing and stretch into part, described sealing is stretched into part and is made by olefinic rubber or thermoplastic resin, sealing is stretched into part and is had the induction pipe and the two sided sealing inclined-plane that are integrally molded, described two sided sealing inclined-plane makes described sealing stretch into part to slip into endpiece and the inlet end of described parabola shaped tubular cylinder, to form uniform zero dead volume wedge type sealing; The outside surface that part is stretched in described sealing has the corrosion-resistant coating of anti-soda acid, deposits double-layer seal layer on described corrosion-resistant coating;
Described column cap comprises the sieve plate in ring flange, bolt, gathering sill, butterfly O-ring seal, distribution plate and the non-equilateral triangle of multiple isosceles hole; Column cap is connected with parabola shaped tubular cylinder inlet end with bolt with ring flange;
Described post tail comprises with the O-ring seal of annular groove, sieve plate, ring flange and bolt with multiple equilateral triangles hole; Post tail and parabola shaped tubular cylinder endpiece are with ring flange and bolt seal.
Wherein, described far ultraviolet post jamb is to the distance 90mm≤L≤100mm of focus.
The above is only the preferred embodiment of the present invention; for those skilled in the art; without departing from the inventive concept of the premise, open and can make some improvements and modifications, within these improve and also can be considered as protection scope of the present invention with retouching.
Claims (4)
1. a parabola shaped high-performance liquid chromatogram preparation column, is characterized in that, described parabola shaped high-performance liquid chromatogram preparation column comprises: be positioned at column cap, the parabola shaped tubular cylinder on cylinder top and be positioned at the post tail of cylinder bottom; Wherein, column cap and post tail are assemblied in respectively import and the outlet of parabola shaped cylinder by flange;
Described parabola shaped tubular cylinder has that internal diameter reduces gradually and the opening shape flattened oval type inlet end of even transition, and internal diameter increases the flat rhombus endpiece of opening shape of even transition gradually, described inlet end is with the inlet tube being integrally molded with described parabola shaped tubular cylinder, described endpiece is with the outlet being integrally molded with described parabola shaped tubular cylinder, and to keep, liquid phase mobile phase first reduces equably, flow out to rear increase parabola shaped tubular cylinder;
The inlet end internal diameter 2R=100mm～150mm of described parabola shaped tubular cylinder, endpiece internal diameter 2r=30mm～60mm, column length L=85cm～95cm, the shape of parabola shaped tubular cylinder is specially: burnt accurate apart from 0.1≤p≤100, far ultraviolet post jamb is to the distance 10mm≤L≤10000mm of focus; Wherein, the parabolic equation of described parabola shaped cylinder is corresponding with different in flow rate, and wherein, Jiao of the larger correspondence of flow velocity is accurate apart from the less parabola shaped tubular cylinder of distance larger, that far ultraviolet post jamb arrives focus;
The inlet end of described parabola shaped tubular cylinder and endpiece have respectively a sealing and stretch into part, described sealing is stretched into part and is made by olefinic rubber or thermoplastic resin, sealing is stretched into part and is had the induction pipe and the two sided sealing inclined-plane that are integrally molded, described two sided sealing inclined-plane makes described sealing stretch into part to slip into endpiece and the inlet end of described parabola shaped tubular cylinder, to form uniform zero dead volume wedge type sealing; The outside surface that part is stretched in described sealing has the corrosion-resistant coating of anti-soda acid, deposits double-layer seal layer on described corrosion-resistant coating;
Described column cap comprises the sieve plate in ring flange, bolt, gathering sill, butterfly O-ring seal, distribution plate and the non-equilateral triangle of multiple isosceles hole; Column cap is connected with parabola shaped tubular cylinder inlet end with bolt with ring flange;
Described post tail comprises with the O-ring seal of annular groove, sieve plate, ring flange and bolt with multiple equilateral triangles hole; Post tail and parabola shaped tubular cylinder endpiece are with ring flange and bolt seal.
2. parabola shaped high-performance liquid chromatogram preparation column according to claim 1, it is characterized in that: described distribution plate is that the polymer organic plastics punching that 0.12～0.15mm is thick becomes the disk with otch, distribution plate middle body has all dispensing orifices identical with the equilateral triangle hole of described post tail sheet plate of shape and number, and multiple adjacent fin juts, this fin jut can accurately be located described distribution plate, and controls distribution plate accurately switching in real time when closing with latched position.
3. parabola shaped high-performance liquid chromatogram preparation column according to claim 1, is characterized in that: described burnt accurate apart from 10≤p≤20.
4. according to the arbitrary described parabola shaped high-performance liquid chromatogram preparation column of claim 1-3, it is characterized in that: described far ultraviolet post jamb is to the distance 90mm≤L≤100mm of focus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110071563.6A CN102628841B (en) | 2011-03-24 | 2011-03-24 | Parabolic high-performance liquid chromatographic preparative column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110071563.6A CN102628841B (en) | 2011-03-24 | 2011-03-24 | Parabolic high-performance liquid chromatographic preparative column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102628841A CN102628841A (en) | 2012-08-08 |
| CN102628841B true CN102628841B (en) | 2014-04-16 |
Family
ID=46587137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110071563.6A Expired - Fee Related CN102628841B (en) | 2011-03-24 | 2011-03-24 | Parabolic high-performance liquid chromatographic preparative column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102628841B (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5416476B2 (en) * | 1971-09-08 | 1979-06-22 | ||
| CH675212A5 (en) * | 1988-03-23 | 1990-09-14 | Ciba Geigy Ag | Liq. chromatography column - with specified cone angle from inflow to outflow |
| US20020008058A1 (en) * | 1999-06-03 | 2002-01-24 | Kerry D. Nugent | Tapered bore column for high performance liquid chromatography |
| CN1204402C (en) * | 2002-03-07 | 2005-06-01 | 中国科学院大连化学物理研究所 | Efficient liquid-phase conic chromatographic column |
| CN201532386U (en) * | 2009-10-13 | 2010-07-21 | 苏州汇通色谱分离纯化有限公司 | Two section type taper liquid chromatography preparation post |
-
2011
- 2011-03-24 CN CN201110071563.6A patent/CN102628841B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102628841A (en) | 2012-08-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100573142C (en) | A kind of reusable liquid-phase chromatographic column | |
| CN102621259B (en) | Capillary parabolic shape capillary liquid chromatography preparative column | |
| CN201532386U (en) | Two section type taper liquid chromatography preparation post | |
| CN105617714B (en) | Three zone Simulation moving beds of asynchronised handover | |
| CN1204402C (en) | Efficient liquid-phase conic chromatographic column | |
| CN102323360A (en) | Annular sieve plate for liquid chromatographic column | |
| CN206450638U (en) | The GM1 detection chromatographic column of high-purity | |
| CN102628841B (en) | Parabolic high-performance liquid chromatographic preparative column | |
| AU2012260981B2 (en) | Method and apparatus for improved resolution chromatography | |
| CN207153163U (en) | A kind of combined type column chromatography device | |
| CN105327524B (en) | A kind of two ends contraction type in smoothing junction is high, middle pressure chromatographic column | |
| CN209043854U (en) | A kind of pre-assembled capillary chromatographic column | |
| CN204293909U (en) | A kind of novel high-resolution chromatography post | |
| CN201522480U (en) | Spherical high-performance liquid chromatogram preparation column | |
| CN202478652U (en) | Chromatographic column | |
| CN205990393U (en) | Unicellular separation based on micro-fluidic chip and containment device | |
| CN102062765B (en) | Column head structure of conical preparative liquid chromatographic column | |
| CN104749296A (en) | Liquid chromatographic column for analysis | |
| CN101629939A (en) | Equal-row chromatographic column and chromatographic analysis method | |
| CN208049728U (en) | It is a kind of to prepare chromatographic grade mixer | |
| CN108744594A (en) | It is a kind of it is novel can automatic feed liquor chromatographic apparatus | |
| CN203124124U (en) | Cyclone separator with good steam-liquid separation effect | |
| CN201692691U (en) | Chromatographic column pressuring piston body | |
| CN203342439U (en) | Chromatographic column | |
| CN203643414U (en) | Liquid-phase chromatographic column for analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: Xinghu Street Industrial Park of Suzhou city in Jiangsu province 215125 BioBAY No. 218 building A2 room 112 Patentee after: SOOCHOW HIGH TECH CHROMATOGRAPHY CO.,LTD. Address before: 228, room 1326, Binhe Road, hi tech Zone, Jiangsu, Suzhou, 215011 Patentee before: SOOCHOW HIGH TECH CHROMATOGRAPHY CO.,LTD. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210628 Address after: Room a12-33, floor 5, building a, business incubation center, Haikou national high tech Zone, 266 Nanhai Avenue, Haikou, Hainan 570102 Patentee after: Makowangzhi (Hainan) Technology Co.,Ltd. Address before: 215125 room 112, A2 / F, bio nano Park, 218 Xinghu street, Suzhou Industrial Park, Jiangsu Province Patentee before: SOOCHOW HIGH TECH CHROMATOGRAPHY Co.,Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140416 |