CN102626183B - Feed additive for improving output and quality of silkworm cocoons and preparation method thereof - Google Patents
Feed additive for improving output and quality of silkworm cocoons and preparation method thereof Download PDFInfo
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- CN102626183B CN102626183B CN 201210094376 CN201210094376A CN102626183B CN 102626183 B CN102626183 B CN 102626183B CN 201210094376 CN201210094376 CN 201210094376 CN 201210094376 A CN201210094376 A CN 201210094376A CN 102626183 B CN102626183 B CN 102626183B
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Abstract
The invention discloses a preparation method for a feed additive for improving output and quality of silkworm cocoons, which comprises the following steps: inoculating strains of D. radiopugnance with the preservation number of ATCC 19172 on a solid medium to be cultivated, and then picking single bacterial colony to be inoculated on a liquid medium to obtain activated seed bacterium liquid; inoculating the activated seed bacterium liquid on a fermentation medium according to the inoculation amount of 4.5% to 6.0%, and cultivating for 60-72 hours at the temperature of 28 DEG C to 32 DEG C to obtain fermentation liquid; and centrifuging the fermentation liquid, laying obtained thallus of the D. radiopugnance to enable the thickness to be from 0.1cm to 1.0cm, then processing the obtained thallus of the D. radiopugnance for 40-60s with the frequency of 600-700MHz and the electric-field strength of 8,000-9,000 V/cm, drying with cold air until the water content is smaller than 8% and obtaining the feed additive for improving output and quality of silkworm cocoons.
Description
Technical field
The invention belongs to the silkworm feed additive technical field, relate to feed addictive of a kind of quantity and quality that improves Cocoon and preparation method thereof.
Background technology
It is an instant effect that the silkworm industry is produced, and the industry that income is large is the important means of pillar and the peasant programme of vast rural economic development; Silk Industry is also traditional foreign exchange earning industry, and export volume occupies first place in the world.Though from eighties of last century since the fifties, the replacing of process silkworm kind etc., the yield and quality of Cocoon has been obtained certain development, but also can not satisfy the development need of current era far away, improve the production performance of silkworm, the yield and quality that increases Cocoon is one of target of pursuing all the time of Silk Industry stakeholder.In order to improve the yield and quality of Cocoon, silkworm industry worker is studied trial from the following aspects both at home and abroad: (1) by the high-voltage electrostatic field processes, physical method processing silkworm egg or the larva such as radioactive ray irradiation; (2) add some nutriments of food by larval phase: as carbohydrate (shell oligosaccharide, threose, sucrose), protein or amino acids, and some other special defects materials (triacontanol, magnetized water, trace element etc.); (3) add food hormone (mixture of juvenile hormone or juvenile hormone and moulting hormone) by larval phase; (4) by changing the raising conditions such as humiture and light, these have all improved the yield and quality of Cocoon to a certain extent.But there is following shortcoming simultaneously in said method: by the high-voltage electrostatic field process, when the physical method such as radioactive ray irradiation is processed silkworm egg or larva, the instrument and equipment cost of use is higher; Freshen sugar class, trace element, magnetized water, triacontanol, urea etc. and exist composition single or similar, effect is not good enough, and economic benefit is not obvious; Hormone adds food to be waited and need pass through the length of time by the prolongation silkworm larva; And increase by changing the raising conditions such as humiture and illumination the uncertainty that there is volume increase in Cocoon quantity and quality, and the operation more complicated etc., so all be not widely applied in actual production at present.In view of the foregoing, urgently wish a kind of source wider, cost compare is low, and is easy and simple to handle, and the metastable feed addictive that can improve the Cocoon yield and quality of effect.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of feed addictive that improves silkworm product cocoon quantity and quality and preparation method thereof.
In order to solve the problems of the technologies described above, the invention provides a kind of preparation method that silkworm produces the feed addictive of cocoon quantity and quality that improves, comprise the following steps:
1., the bacterial classification with radioresistant bacterium (D.radiopugnance) is seeded on the solid medium that is fit to radioresistant bacterium, cultivate 34～38h in 28～32 ℃, then picking list colony inoculation is to the fluid nutrient medium that is fit to radioresistant bacterium, be cultured to exponential phase in 28～32 ℃, get the kind daughter bacteria liquid after activation;
The preserving number of radioresistant bacterium (D.radiopugnance) is ATCC 19172;
2., with step 1. the kind daughter bacteria liquid after the activation of gained by volume 4.5%～6.0% inoculum concentration be seeded on the fermentation medium that is fit to radioresistant bacterium, cultivate 60～72h under 28～32 ℃ of conditions; Get zymotic fluid;
3., with step 2. the zymotic fluid of gained carry out centrifugally, obtain the radioresistant bacterium thalline; The radioresistant bacterium thalline is tiled into 0.1cm～1.0cm thickness, at frequency 600～700MHz, processes 40～60s under the condition of electric-field intensity 8000～9000V/cm; Again under 5～35 ℃, the condition of wind speed 1.5～2.0m/s, relative humidity 10～20% cold air drying to water content less than 8% (mass ratio), the silkworm that is improved produces the feed addictive of cocoon quantity and quality.
Produce preparation method's the improvement of the feed addictive of cocoon quantity and quality as raising of the present invention silkworm:
The solid medium that is fit to radioresistant bacterium is the TGY solid medium;
The fluid nutrient medium that is fit to radioresistant bacterium is the TGY fluid nutrient medium;
The fermentation medium that is fit to radioresistant bacterium is defatted soybean culture medium or TGY fluid nutrient medium;
The defatted soybean culture medium of every 1000mL consists of the following composition: fineness is 60-80 purpose defatted soy flour 20g, glucose 2g, and sodium nitrate 2g, yeast extract 1g, all the other are distilled water.
Produce preparation method's the further improvement of the feed addictive of cocoon quantity and quality as raising of the present invention silkworm: centrifugal in 3. of step is: the centrifugal 4～6min of 4000～6000rpm.
The present invention also discloses the feed addictive that the raising silkworm that utilizes the said method preparation and get produces the cocoon quantity and quality simultaneously.
The feed addictive of raising Cocoon quantity and quality provided by the present invention contains the radioresistant bacterium through break process.It is Deinococcus radiopugnance (D.radiopugnance) ATCC 19172 that this radioresistant bacterium is preferably bacterial strain.Above-mentioned preserving number is that the radioresistant bacterium (D.radiopugnance) of ATCC 19172 has clearly in the patent No. is 200610154663.4 invention " preparation method of tool uciferase activity radioresistant bacterium " (authorizing) and informs.
The present invention's TGY solid medium used, the conventional medium that the TGY fluid nutrient medium is the industry.
Feed addictive of the present invention shows through feeding experiment repeatedly: product characteristics are stable, have safe and efficient characteristic, and easy and simple to handle; During actual the use, adding when food at mulberry leaf, to be mixed with concentration be that the spray solution of 0.2～0.5% (mass ratio) is sprayed on mulberry leaf and (evenly is sprayed at blade back two sides, mulberry leaf blade face with sprayer, do not drip to squirt and be advisable), addition with 0.3～0.6% (w/w) in man-made feeds freshens food, can obviously improve cocoon weight (improving 7.14%～10.96%) and the cocoon shell weight (improving 11.13%～16.12%) of silkworm.Additive of the present invention has more significant economic benefit and social benefit, and market prospects are wide.
The specific embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1, a kind of preparation method who improves the feed addictive of silkworm product cocoon quantity and quality comprise the following steps:
1, the acquisition of radioresistant bacterium bacterial strain
Preserving number used is the bacterial strain D.radiopugnance of the radioresistant bacterium of ATCC N0.19172, provided by American Type Culture Collecti.
2, the activation of bacterial classification
Above-mentioned radioresistant bacterium streak inoculation on the TGY solid medium, is cultivated 36h for 30 ℃, and the TGY Liquid Culture of picking list colony inoculation in triangular flask is cultured to exponential phase based on 30 ℃; Kind daughter bacteria liquid after must activating.
The preparation of TGY fluid nutrient medium: the TGY fluid nutrient medium of every 1000mL consists of peptone 6g, yeast extract 3g, and glucose 1g, adding distil water is to 1000mL; Then carry out conventional high-temperature sterilization (1.1 atmospheric pressure, 20min sterilizes under 121 ℃).
The preparation of TGY solid medium: the TGY solid medium of every 1000mL consists of: peptone 6g, and yeast extract 3g, glucose 1g, agar 15g, adding distil water is to 1000mL; Then carry out conventional high-temperature sterilization (1.1 atmospheric pressure, 20min sterilizes under 121 ℃).
3, fermented and cultured
With carrying out fermented and cultured in the culture medium (defatted soybean culture medium) that to be the access of 5% inoculum concentration by volume be mixed with defatted soybean of the kind daughter bacteria liquid after the activation of step 2 gained, cultivate 60h, get zymotic fluid (thalline zymotic fluid) for 30 ℃; The preparation of defatted soybean culture medium: the defatted soybean culture medium of every 1000mL consists of: fineness is 60-80 purpose defatted soy flour 20g, glucose 2g, and sodium nitrate 2g, yeast extract 1g, adding distil water is to 1000mL; Then carry out conventional high-temperature sterilization (1.1 atmospheric pressure, 20min sterilizes under 121 ℃).
4, the fragmentation of radioresistant bacterium
Step 3 is cultivated the thalline zymotic fluid that obtains, in the centrifugal 5min of 5000rpm, remove supernatant, the radioresistant bacterium wet thallus is paved into equably the thickness of 0.5cm, at frequency 600MHz, processed 40 seconds under the condition of electric-field intensity 8000V/m, make clasmatosis, obtain the radioresistant bacterium through fragmentation.
5, the preparation of feed addictive
The above-mentioned radioresistant bacterium that obtains through break process is placed in material disc, put into and carry out cold air drying in cold wind drier, chilling temperature is controlled at 20-25 ℃, wind speed 1.5-2.0m/s, relative humidity 10-15%, be dried to water content 6-8% (quality %), obtain the feed addictive for improving silkworm product cocoon quantity and quality of the present invention.
Experiment 1-1, effect
Control group adopts mulberry leaf to raise, test group is: the feed addictive of embodiment 1 gained is mixed with the spray solution that concentration is 0.2% (mass ratio), be sprayed on mulberry leaf when mulberry leaf add food and (evenly be sprayed at blade back two sides, mulberry leaf blade face with sprayer, do not drip to squirt and be advisable), can feed silkworm after slightly doing to dry.Freshen the food time since 3 ages silkworm until put on an arrow, totally 150 of test silkworms, every group each 25 (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The mulberry leaf consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 1.
The impact on the silkworm cocoon quality of table 1, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.518±0.232A | 0.535±0.082A | 21.3 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Experiment 1-2, effect
Control group adopts mulberry leaf to raise, test group is: the feed addictive of embodiment 1 gained is mixed with the spray solution that concentration is 0.2% (mass ratio), be sprayed on mulberry leaf when mulberry leaf add food and (evenly be sprayed at blade back two sides, mulberry leaf blade face with sprayer, do not drip to squirt and be advisable), can feed silkworm after slightly doing to dry.Freshen the food time since 4 ages silkworm until put on an arrow, totally 148 of test silkworms, every group of each 24-25 head (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The mulberry leaf consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 2.
The impact on the silkworm cocoon quality of table 2, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.498±0.227A | 0.527±0.054A | 21.1 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Experiment 1-3, effect
Control group adopts mulberry leaf to raise, test group is: the feed addictive of embodiment 1 gained is mixed with the spray solution that concentration is 0.2% (mass ratio), be sprayed on mulberry leaf when mulberry leaf add food and (evenly be sprayed at blade back two sides, mulberry leaf blade face with sprayer, do not drip to squirt and be advisable), can feed silkworm after slightly doing to dry.Freshen the food time since 5 ages silkworm until put on an arrow, totally 150 of test silkworms, every group each 25 (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The mulberry leaf consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 3.
The impact on the silkworm cocoon quality of table 3, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.461±0.239A | 0.517±0.051A | 21.0 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Experiment 1-4, effect
Control group adopts man-made feeds to raise, and test group is: the feed addictive that adds 0.3% (w/w) embodiment, 1 gained in man-made feeds.Freshen the food time since 3 ages silkworm until put on an arrow, totally 146 of test silkworms, every group of each 23-25 head (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The man-made feeds consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 4.
The impact on the silkworm cocoon quality of table 4, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.011±0.216B | 0.397±0.044B | 19.7 |
| Test group | 2.217±0.223A | 0.461±0.051A | 20.8 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Experiment 1-5, effect
Control group adopts man-made feeds to raise, and test group is: the feed addictive that adds 0.3% (w/w) embodiment, 1 gained in man-made feeds.Freshen the food time since 4 ages silkworm until put on an arrow, totally 150 of test silkworms, every group each 25 (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The man-made feeds consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 5.
The impact on the silkworm cocoon quality of table 5, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.011±0.216B | 0.397±0.044B | 19.7 |
| Test group | 2.213±0.243A | 0.454±0.043A | 20.5 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Experiment 1-6, effect
Control group adopts man-made feeds to raise, and test group is: the feed addictive that adds 0.3% (w/w) embodiment, 1 gained in man-made feeds.Freshen the food time since 5 ages silkworm until put on an arrow, totally 147 of test silkworms, every group of each 24-25 head (that is, control group and test group respectively arrange 3 groups), experimental cultivar is rich 1 * 54A.The man-made feeds consumption of every silkworm of control group and test group is identical.The mean value of the effect of 2 groups sees Table 6.
The impact on the silkworm cocoon quality of table 6, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.011±0.216B | 0.397±0.044B | 19.7 |
| Test group | 2.168±0.221A | 0.442±0.050A | 20.4 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Embodiment 2, a kind of preparation method who improves the feed addictive of silkworm product cocoon quantity and quality comprise the following steps:
1, the acquisition of radioresistant bacterium bacterial strain
With embodiment 1.
2, the activation of bacterial classification
Above-mentioned radioresistant bacterium streak inoculation on the TGY solid medium, is cultivated 34h for 32 ℃, and the TGY Liquid Culture of picking list colony inoculation in triangular flask is cultured to exponential phase based on 32 ℃; Kind daughter bacteria liquid after must activating.
The preparation of TGY fluid nutrient medium, TGY solid medium are prepared all with embodiment 1.
3, fermented and cultured
With carrying out fermented and cultured in the culture medium (defatted soybean culture medium) that to be the access of 6% inoculum concentration by volume be mixed with defatted soybean of the kind daughter bacteria liquid after the activation of step 2 gained, cultivate 65h, get zymotic fluid (thalline zymotic fluid) for 32 ℃.The preparation of defatted soybean culture medium is with embodiment 1.
4, the fragmentation of radioresistant bacterium
Step 3 is cultivated the thalline zymotic fluid that obtains, in the centrifugal 4min of 6000rpm, remove supernatant, the radioresistant bacterium wet thallus is paved into equably the thickness of 1cm, at frequency 700MHz, processed 60 seconds under the condition of electric-field intensity 9000V/m, make clasmatosis, obtain the radioresistant bacterium through fragmentation.
5, the preparation of feed addictive
The above-mentioned radioresistant bacterium that obtains through break process is placed in material disc, put into and carry out cold air drying in cold wind drier, chilling temperature is controlled at 5-15 ℃, wind speed 1.5-2.0m/s, relative humidity 10-20%, be dried to water content 6-8% (quality %), obtain the feed addictive for improving silkworm product cocoon quantity and quality of the present invention.
Experiment 2, effect
With the feed addictive of feed addictive alternate embodiment 1 gained of embodiment 2 gained, all the other are with experiment 1-1; The mean value of the effect of 2 groups sees Table 7.
The impact on the silkworm cocoon quality of table 7, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.516±0.232A | 0.532±0.082A | 21.2 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Embodiment 3, a kind of preparation method who improves the feed addictive of silkworm product cocoon quantity and quality comprise the following steps:
1, the acquisition of radioresistant bacterium bacterial strain
With embodiment 1.
2, the activation of bacterial classification
Above-mentioned radioresistant bacterium streak inoculation on the TGY solid medium, is cultivated 38h for 28 ℃, and the TGY Liquid Culture of picking list colony inoculation in triangular flask is cultured to exponential phase based on 28 ℃; Kind daughter bacteria liquid after must activating.
The preparation of TGY fluid nutrient medium, TGY solid medium are prepared all with embodiment 1.
3, fermented and cultured
Be to carry out fermented and cultured in 4.5% inoculum concentration access defatted soybean culture medium by volume with the kind daughter bacteria liquid after the activation of step 2 gained, cultivate 65h, get zymotic fluid (thalline zymotic fluid) for 32 ℃.The preparation of defatted soybean culture medium is with embodiment 1.
4, the fragmentation of radioresistant bacterium
Step 3 is cultivated the thalline zymotic fluid that obtains, in the centrifugal 6min of 4000rpm, remove supernatant, the radioresistant bacterium wet thallus is paved into equably the thickness of 0.2cm, at frequency 650MHz, processed 50 seconds under the condition of electric-field intensity 8500V/m, make clasmatosis, obtain the radioresistant bacterium through fragmentation.
5, the preparation of feed addictive
The above-mentioned radioresistant bacterium that obtains through break process is placed in material disc, put into and carry out cold air drying in cold wind drier, chilling temperature is controlled at 25-30 ℃, wind speed 1.5-2.0m/s, relative humidity 10-15%, be dried to water content 6-8% (quality %), obtain the feed addictive for improving silkworm product cocoon quantity and quality of the present invention.
Experiment 3, effect
With the feed addictive of feed addictive alternate embodiment 1 gained of embodiment 3 gained, all the other are with experiment 1-1; The mean value of the effect of 2 groups sees Table 8.
The impact on the silkworm cocoon quality of table 8, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.515±0.232A | 0.531±0.082A | 21.1 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
Embodiment 4, with embodiment 3 steps 3) defatted soybean culture medium in fermented and cultured makes the TGY fluid nutrient medium into, all the other are with embodiment 3.
Experiment 4, effect
With the feed addictive of feed addictive alternate embodiment 1 gained of embodiment 4 gained, all the other are with experiment 1-1; The mean value of the effect of 2 groups sees Table 9.
The impact on the silkworm cocoon quality of table 9, feed addictive of the present invention
| Whole cocoon heavy (g) | Cocoon layer heavy (g) | Cocoon layer rate (%) | |
| Control group | 2.297±0.254B | 0.465±0.059B | 20.2 |
| Test group | 2.510±0.232A | 0.512±0.082A | 20.4 |
Annotate: in same example, the different capitalizations of mark represent difference extremely significantly (P＜0.01).
At last, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (4)
1. improve the preparation method that silkworm produces the feed addictive of cocoon quantity and quality, it is characterized in that comprising the following steps:
1., the bacterial classification with radioresistant bacterium (D. radiopugnance) is seeded on the solid medium that is fit to radioresistant bacterium, cultivate 34 ~ 38 h in 28 ~ 32 ℃, then picking list colony inoculation is to the fluid nutrient medium that is fit to radioresistant bacterium, be cultured to exponential phase in 28 ~ 32 ℃, get the kind daughter bacteria liquid after activation;
The preserving number of described radioresistant bacterium (D. radiopugnance) is ATCC 19172;
2., with step 1. the kind daughter bacteria liquid after the activation of gained by volume 4.5% ~ 6.0% inoculum concentration be seeded on the fermentation medium that is fit to radioresistant bacterium, cultivate 60 ~ 72 h under 28 ~ 32 ℃ of conditions; Get zymotic fluid;
3., with step 2. the zymotic fluid of gained carry out centrifugally, obtain the radioresistant bacterium thalline; Described radioresistant bacterium thalline is tiled into 0.1 cm ~ 1.0 cm thickness, at frequency 600 ~ 700 MHz, processes 40 ~ 60 s under the condition of electric-field intensity 8000 ~ 9000 V/cm; Again under 5 ~ 35 ℃, the condition of wind speed 1.5 ~ 2.0 m/s, relative humidity 10 ~ 20% cold air drying to water content less than 8% mass ratio, the silkworm that is improved produces the feed addictive of cocoon quantity and quality.
2. raising according to claim 1 silkworm produces the preparation method of the feed addictive of cocoon quantity and quality, it is characterized in that:
The solid medium of described suitable radioresistant bacterium is the TGY solid medium;
The fluid nutrient medium of described suitable radioresistant bacterium is the TGY fluid nutrient medium;
The fermentation medium of described suitable radioresistant bacterium is defatted soybean culture medium or TGY fluid nutrient medium;
The defatted soybean culture medium of described every 1000 mL consists of the following composition: fineness is 60-80 purpose defatted soy flour 20 g, glucose 2 g, and sodium nitrate 2 g, yeast extract 1 g, all the other are distilled water.
3. raising according to claim 2 silkworm produces the preparation method of the feed addictive of cocoon quantity and quality, it is characterized in that: centrifugal in 3. of described step is: centrifugal 4 ~ 6 min of 4000 ~ 6000 rpm.
4. utilize as arbitrary in claim 1 ~ 3 as described in the method preparation and the feed addictive of raising silkworm product cocoon quantity and quality.
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| CN103719642B (en) * | 2013-12-26 | 2015-07-08 | 浙江大学 | Mulberry leaf storage preservative with effect of increasing silkworm cocoon yield |
| CN103983726B (en) * | 2014-05-29 | 2016-02-17 | 西南大学 | Glycocoll or its metabolism regulators are in the application improved in domestic silkworm silk output and method thereof |
| CN104798737B (en) * | 2014-12-10 | 2017-06-06 | 张烨 | A kind of silkworm cultural method |
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| CN1385092A (en) * | 2002-04-15 | 2002-12-18 | 山东农业大学 | Puffed granular fodder for silkworm |
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| CN1385092A (en) * | 2002-04-15 | 2002-12-18 | 山东农业大学 | Puffed granular fodder for silkworm |
| CN1810148A (en) * | 2006-02-27 | 2006-08-02 | 何宜斌 | Nutritious silkworm feed |
| CN101181022A (en) * | 2007-12-05 | 2008-05-21 | 浙江大学 | Folium mori homogenate wet body feedstuff for domestic silkworm and preparation method thereof |
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