CN102626033A - Method utilizing nitrilotriacetic acid (NTA) to improve planting effects of ryegrass turf in garbage compost base material - Google Patents
Method utilizing nitrilotriacetic acid (NTA) to improve planting effects of ryegrass turf in garbage compost base material Download PDFInfo
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- CN102626033A CN102626033A CN2012101348569A CN201210134856A CN102626033A CN 102626033 A CN102626033 A CN 102626033A CN 2012101348569 A CN2012101348569 A CN 2012101348569A CN 201210134856 A CN201210134856 A CN 201210134856A CN 102626033 A CN102626033 A CN 102626033A
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Abstract
The invention relates to a method utilizing nitrilotriacetic acid (NTA) to improve planting effects of ryegrass turfs in a garbage compost base material, which comprises adding prepared 20g of compost in each culture dish, and applying the nitrilotriacetic acid with the concentration to be 5-15mmol/kg-1 in the compost base material; sowing 50 ryegrass seeds in each culture dish; placing each culture dish where the seeds are sown in a laboratory to germinate, watering for one time in the morning and evening each day, often exchanging the positions of the culture dishes, guaranteeing that illumination is consistent, and determining germination rate and germination index on the seventh day after germination. Study results show that the low-concentration NTA can promote germination of lawn plants, the NTA of 5-15mmol/kg-1 can improve germination rate and germination index of the ryegrass seeds, but germination of the seeds is restrained along with further increase of the concentration of the NTA. When the concentration of the NTA is 25mmol/kg-1, germination of the lawn plants can be restrained remarkably.
Description
Technical field
The invention belongs to garbage compost in urban lawn build and plant technology Application for Field, relate to the garbage compost heavy metal and under the effect of chelating agent, move and corresponding control to the deep soil leaching.Say so more specifically and adopt the nitrilotriacetic acid chelating agent to improve the method for perennial ryegrass garbage compost seed germination rate.
Background technology
In recent years, the problem of environmental pollution that is caused by house refuse has become one of focus of social extensive concern.Along with the raising day by day of China's living standards of the people, the domestic waste generation also increases thereupon.Consumer garbage compost as lawn matrix, not only can be avoided food chain, solved the problem of outlet of rubbish simultaneously again.Garbage compost has good physicochemical property, abundant nutrient, is to substitute the more satisfactory material of soil as lawn matrix.Populous in China, land resources is poor relatively, has more realistic meaning with garbage compost as lawn matrix.
At present, China has tentatively launched consumer garbage compost is used for the research work of greenery patches, gardens matrix or fertilizer, and garbage compost adds soil with the form of fertilizer usually, can improve soil property, improves turfgrass biomass, chlorophyll content etc.But the heavy metal that contains high concentration in the garbage compost when it improves soil, edaphophyte and microorganism, also produces harm to them.So, propose garbage compost to be applied to the actual idea that must remove heavy metal wherein if want.In the garbage compost application, how to prevent or reduce heavy metal to get into soil and underground water generation, become a big problem that will solve.
Chelation evoked recovery technique " (chelate-induced phytoremediation), promptly add chelating agent and the heavy metal that is combined by solid phase of soil is discharged again and enter into soil through manual work.Chelating agent commonly used at present can be divided into 3 big types: the one, and many carboxyaminos of synthetic acids chelating agent is like EDTA, DTPA, CDTA etc.; The 2nd, natural many carboxyaminos acids chelating agent is like EDDS, NTA; The 3rd, natural organic sequestering agent like some small molecular organic acids, comprises citric acid, malic acid, histidine, oxalic acid etc.; The 4th, inorganic complexing agent mainly comprises rhodanide, chloride etc.
Prior art research shows that adding EDTA can significantly improve the enriching quantity of heavy metal in supplying the examination lawn plant.But when utilizing EDTA to work in coordination with phytoremediation; Because the compound that EDTA and EDTA and heavy metal chelating form has very strong dissolubility and bio-toxicity; And in soil, be difficult to, so cause environmental problems such as polluting deep soil and groundwater contamination possibly by light, chemical substance and biological degradation.But NTA (Nitrilotriacetic acid) nitrilotriacetic acid (sodium salt) was applied to scale remover in 50 years in the past always, in analytical chemistry, was used as complexometric titration agent and screening agent, Chang Zuowei activator in catalytic kinetics is analyzed.Although the NTA sequestering power than EDTA a little less than, compare with low-molecular-weight organic acid (citric acid, malic acid etc.), be still a kind of stronger chelating agent.Therefore, the NTA of low concentration not only can be used as chelating agent and promotes lawn plant to the absorption of heavy metal but also can not suppress the growth of plant.
In view of above-mentioned analysis, adopt chelating agent NTA to join and improve garbage compost matrix perennial ryegrass turf planting effect in the garbage compost, be that a kind of unexpected NTA utilizes approach.That is to say through this technology, can fully satisfy the lawn plant growth needs, improve turf planting effect, can solve the reparation problem of garbage compost again.
Summary of the invention
The invention discloses the method that a kind of NTA of employing improves garbage compost matrix perennial ryegrass turf planting effect, it is characterized in that being undertaken by following step:
(1) be to add compost 20 g that get ready in the culture dish of 6 cm at diameter, in composting substrate, applying concentration is the 5-15mmolkg-1 nitrilotriacetic acid;
(2) ryegrass seed of selection full seed, 50 in every ware;
(3) will broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish; Assurance illumination is consistent; Laboratory temperature is 13.4 ℃ ~ 18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 450-700 μ mol m-2 s-1;
(4) when sprouting 7d, measure germination rate and germination index.
Compost of the present invention refers to: consumer garbage compost, air-dry after, cross 2 mm sieve, subsequent use.
The concentration that the present invention preferably applies nitrilotriacetic acid is 5-10mmolkg
-1Being more preferably the concentration that applies nitrilotriacetic acid is 5 mmolkg
-1
The present invention further discloses 5-10mmolkg
-1The application of amino carboxy chelating agent aspect raising garbage compost matrix perennial ryegrass turf planting effect.
Research of the present invention shows: be planted in the NTA that perennial ryegrass on the garbage compost applies variable concentrations, find that low concentration NTA can promote lawn plant to sprout 5-10mmolkg
-1NTA can improve the germination rate and the germination index of ryegrass seed, but along with NTA concentration improves again, can suppress the germination of seed, when NTA concentration is 25 mmolkg
-1The time, can significantly suppress the sprouting of lawn plant.
The more detailed preparation method of the present invention is following:
1 experiment material and method
1.1 experiment material
The common lawn plant of northern China is selected in this experiment for use, perennial ryegrass (
Lolium perenneL.).Culture matrix is a consumer garbage compost, air-dry after, cross 2 mm sieve, subsequent use.The compost physicochemical property is: pH 7.62, the content of organic matter 12.12%, full nitrogen 5.18%, available phosphorus 77.92 mgkg
-1, full potassium 50.83 gkg
-1, saturation moisture content 0.76 mlg
-1, unit weight 0.85 gml
-1Heavy metal (Cd, Cr, Cu, Pb, Zn) content is respectively 1.97,67.00,238.73,172.11 and 496.38
μGg
-1
1.2 lawn plant is cultivated
At diameter is to add compost 20 g that get ready in the culture dish of 6 cm.In composting substrate, apply NTA, its processing horizontal is respectively: 0,5,10,15,20 and 25 mmolkg
-1Compost.Select the ryegrass seed of full seed, 50 in every ware, experiment is 3 repetitions.To broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish, guarantees that illumination is consistent.Laboratory temperature is 13.4 ℃ ~ 18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 450-700
μMol m
-2s
-1
1.3 research method
(1) the seed germination index of correlation is measured
Seed base-root grows and half is regarded as sprouting above seed length, when sprouting 7d, measures germination rate and germination index.
Germination rate
Gr=(subnumber is planted experimentally in chitting piece number/confession) * 100%.Germination index
GI=Σ (
Gt/
Dt)
Wherein
GIBe germination index,
GtBe chitting piece number in t day,
DtBe corresponding germination fate.
(2) growth of seedling index determining
The every 5d in back that germinates measures a plant height.Sow and cradle after 28 days, oven dry, on the geodetic with underground part biomass dry weight.
(3) data
Data analysis adopts EXCEL 2003 and SPSS 11.5 analysis software to handle.
2 results and analysis
2.1 NTA is to the influence of seed germination of lawn plants
After planting the 5th day, ryegrass seed began to sprout.The NTA that applies variable concentrations all is the trend (table 1) of low concentration promotion high concentration inhibition to the influence of the germination rate in the perennial ryegrass.For perennial ryegrass, when NTA concentration is 5 mmolkg
-1The time promoted the sprouting of ryegrass seed, when concentration is higher than 5 mmolkg
-1The time, with the increase of NTA concentration, the inhibitory action that ryegrass seed is sprouted is obvious more, but difference not significantly (
p>0.05).The NTA of low concentration also can improve the sprouting index of ryegrass seed, but works as NTA concentration greater than 20mmolkg
-1The time, to the sprouting generation inhibitory action of ryegrass seed.
Table 1 variable concentrations NTA is to the influence of seed germination of lawn plants index
Annotate: data are represented with mean value ± standard error (SE) in the table; Different letter representations are remarkable at 0.05 level difference in the same row, and same letter representes that difference is not remarkable.(following table together).
2.2 NTA is to the influence of lawn plant plant height
Each NTA concentration is handled down, and table 2 is seen in the growth of lawn plant seedling plant height.Low concentration NTA can improve plant plant height (
p<0.05), at preceding 12 d, when NTA concentration is 10 mmolkg
-1The time, the promotion of plant height is reached maximum, when NTA concentration improves again, the plant strain height is in contrast.After lawn plant grew into 22 d, the processing plant height that applies NTA all was lower than contrast, and NTA concentration is high more, and is obvious more to lawn plant plant height inhibitory action.Other concentration NTA to lawn plant plant height inhibitory action significantly (
p<0.05).
Table 2 variable concentrations NTA is to the influence (cm) of lawn plant plant height
2.3 NTA is to the influence of lawn plant biomass
Variable concentrations NTA sees table 3 to the influence of perennial ryegrass ground and underground biomass.Applying of NTA suppressed perennial ryegrass ground and ground biomass, applies 5,10,15,20 and 25 mmolkg
-1NTA, the ground biomass comparison of perennial ryegrass is according to having reduced by 4.0%, 6.7%, 10.0%, 9.8% and 10.0% respectively.When NTA concentration is higher than 10 mmolkg
-1The time, significantly suppress the perennial ryegrass ground biomass accumulation (
p<0.05).Apply 5,10,15,20 and 25 mmolkg
-1NTA, the underground biomass comparison of perennial ryegrass is according to having reduced by 6.0%, 10.4%, 19.0%, 25.7% and 34.0% respectively.
Table 3 variable concentrations NTA is to the influence (mg/ ware) of perennial ryegrass biomass
3 conclusions
This research finds that through to being planted in the NTA that perennial ryegrass on the garbage compost applies variable concentrations low concentration NTA can promote lawn plant to sprout 5-10mmolkg
-1NTA can improve the germination rate and the germination index of ryegrass seed, but along with NTA concentration improves again, can suppress the germination of seed, when NTA concentration is 25 mmolkg
-1The time, can significantly suppress the sprouting of lawn plant.Therefore, low concentration NTA is little to the plant height influence of perennial ryegrass, but the plant plant height reduces with the rising of NTA concentration.NTA applies the biomass that has also reduced lawn plant, and NTA concentration is high more, and the lawn plant biomass is low more.NTA concentration is high more, and the metal concentration in the plant growth environment is also high more, so the plant primary growth is produced inhibitory action.Comprehensive each index, drawing NTA concentration is 5 mmolkg
-1The time, seed germination of optimum perennial ryegrass and primary growth.
Embodiment
Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Used reagent has commercially available.
Embodiment 1
The common lawn plant of northern China is selected in this experiment for use, perennial ryegrass (
Lolium perenneL.).
Culture matrix is taken from the consumer garbage compost of Tianjin Xiao Dian garbage compost treatment plant, air-dry after, cross 2 mm sieve, subsequent use.The compost physicochemical property is: pH 7.62, the content of organic matter 12.12%, full nitrogen 5.18%, available phosphorus 77.92 mgkg
-1, full potassium 50.83 gkg
-1, saturation moisture content 0.76 mlg
-1, unit weight 0.85 gml
-1Heavy metal (Cd, Cr, Cu, Pb, Zn) content is respectively 1.97,67.00,238.73,172.11 and 496.38
μGg
-1, other embodiment down together.
(1) be to add compost 20 g that get ready in the culture dish of 6 cm at diameter, in composting substrate, applying concentration is nitrilotriacetic acid (NTA) for the 5mmolkg-1 amino carboxy chelating agent.
(2) ryegrass seed of selection full seed, 50 in every ware;
(3) will broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish; Assurance illumination is consistent; Laboratory temperature is 13.4 ℃ ~ 18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 450 μ mol m-2 s-1;
(4) when sprouting 7d, measure germination rate and germination index.
Embodiment 2
(1) be to add compost 20 g that get ready in the culture dish of 6 cm at diameter, in composting substrate, applying concentration is nitrilotriacetic acid (NTA) for the 10mmolkg-1 amino carboxy chelating agent;
(2) ryegrass seed of selection full seed, 50 in every ware;
(3) will broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish; Assurance illumination is consistent; Laboratory temperature is 18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 700 μ mol m-2 s-1;
(4) when sprouting 7d, measure germination rate and germination index.
Embodiment 3
(1) be to add compost 20 g that get ready in the culture dish of 6 cm at diameter, in composting substrate, applying concentration is nitrilotriacetic acid (NTA) for the 15mmolkg-1 amino carboxy chelating agent;
(2) select full seed ryegrass seed, 50 in every ware;
(3) will broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish; Assurance illumination is consistent; Laboratory temperature is 18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 600 μ mol m-2 s-1;
(4) when sprouting 7d, measure germination rate and germination index.
Claims (5)
1. NTA improves the method for garbage compost matrix perennial ryegrass turf planting effect, it is characterized in that being undertaken by following step:
(1) be to add the compost 20g that gets ready in the culture dish of 6 cm at diameter, in composting substrate, applying concentration is the 5-15mmolkg-1 nitrilotriacetic acid;
(2) ryegrass seed of selection full seed, 50 in every ware;
(3) will broadcast seed-bearing culture dish and place sprouting in the laboratory, respectively water 1 time every day sooner or later, often changes the position of each culture dish; Assurance illumination is consistent; Laboratory temperature is 13.4 ℃-18.9 ℃ between culture period, and medial humidity is 55.4%, and intensity of illumination is 450-700 μ mol m
-2s
-1
(4) when sprouting 7d, measure germination rate and germination index.
2. the described method of claim 1, compost wherein refers to: consumer garbage compost, air-dry after, cross 2 mm sieve, subsequent use.
3. the described method of claim 1, the concentration that wherein applies nitrilotriacetic acid is 5-10mmolkg
-1
4. the described method of claim 1, the concentration that wherein applies nitrilotriacetic acid is 5 mmolkg
-1
5.5-10mmolkg
-1The application of amino carboxy chelating agent aspect raising garbage compost matrix perennial ryegrass turf planting effect.
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Application publication date: 20120808 |