CN102613185A - Parahormone promoting plant root elongation and application thereof - Google Patents
Parahormone promoting plant root elongation and application thereof Download PDFInfo
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- CN102613185A CN102613185A CN2012100122692A CN201210012269A CN102613185A CN 102613185 A CN102613185 A CN 102613185A CN 2012100122692 A CN2012100122692 A CN 2012100122692A CN 201210012269 A CN201210012269 A CN 201210012269A CN 102613185 A CN102613185 A CN 102613185A
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Abstract
A parahormone scutellarin promoting plant root elongation, and root elongation effect inhibition of antagonism auxin substances 2,4-D and antagonism methyl jasmonate is provided, and an application of scutellarin in preparation of parahormones for promoting the plant root elongation, and the root elongation effect inhibition of the antagonism auxin substances 2,4-D and the antagonism methyl jasmonate is also provided. The plant root elongation promotion effect of the plant secondary metabolite scutellarin is first disclosed, scutellarin mutually antagonizes with the two phytohormone 2,4-D and methyl jasmonate having the root elongation inhibition effect, and the antagonism effect is dose-dependent, so the plant root elongation effect of scutellarin is a parahormone effect. Above application is a first application of scutellarin in the plant field, so a new field is displayed for the application of scutellarin, thereby the excavation of the value of scutellarin is promoted.
Description
Technical field:
The invention belongs to the auxin field; Particularly; Relate to a kind of promotion plant roots elongation, antagonism auxin substance 2; 4-D suppresses the parahormone scutellarin that root elongation effect, antagonism methyl jasmonate suppress root elongation effect, and scutellarin promotes the plant roots elongation in preparation, at preparation antagonism auxin substance 2,4-D suppresses the root elongation, suppresses the application in the parahormone of root elongation effect at preparation antagonism methyl jasmonate.
Background technology:
Scutellarin is the main active in the short booth bitter fleabane; Present effectiveness research about scutellarin; Mainly the treatment that is used for ischemic angiocardiopathy and cerebrovascular disease on clinical medicine; This medicament has hemangiectasis, improves blood microcirculation, reduces effect (Gao et al.2007 such as vascular resistence and anti-platelet aggregation; Gao et al.2010; Lin et al.2007; Pan et al.2011).In addition; Also have research to show the cytotoxicity (Hong and Liu 2004) that scutellarin can be resisted hydrogen peroxide and causes in recent years, and the protection liver cell is arranged, improve liver function; Alleviate the hepatic tissue pathology extent of damage; Prevent the effects (Tan et al.2007) such as liver fibrosis that carbon tetrachloride brings out, also having report to propose scutellarin all is useful to multiple treatment of diseases, comprises (Chen et al.2006) such as insomnia, depression, pain, amnesias.
The medicinal widely approach of above-mentioned scutellarin explains that it has multiple activity to zooblast.Because plant cell and zooblast have a lot of identical functions processes, the compound that zooblast is had multiple activity is very likely also brought into play important effect to plant cell.And scutellarin derives from plant, and must there be certain meaning in its generation for growth and development of plant.Based on above-mentioned theory understanding, open up the great theory and practice meaning of Secondary Metabolism of Plant thing biological effect in addition, exploring the new purposes of scutellarin on plant physiology is a job that is rich in novelty and development prospect.Yet up to now, the using value of scutellarin all is embodied in medical domain, and does not still have any report about its function and purposes at plant field.
Summary of the invention:
The object of the present invention is to provide a kind of is the parahormone of the promotion plant roots elongation of unique active component with scutellarin, and the application of scutellarin in the parahormone of preparation promotion plant roots elongation is provided.
The parahormone scutellarin of promotion plant roots elongation provided by the present invention derives from the short booth bitter fleabane of feverfew, is a kind of important Secondary Metabolism of Plant product, belongs to flavonoids.
Another object of the present invention provides a kind of parahormone scutellarin, has the effect of other hormone molecules in inhibiting root elongation effects of antagonism.
The parahormone scutellarin of other hormone molecules in inhibiting root elongation effects of antagonism provided by the present invention can be distinguished antagonism auxin substance 2, the root elongation depression effect of 4-D and methyl jasmonate, and also antagonistic effect is a dose dependent.
A kind of parahormone that promotes the plant roots elongation contains scutellarin.
Described parahormone, it is unique active component with scutellarin.
Described parahormone, scutellarin concentration are 10-400 μ M.
Described parahormone, scutellarin concentration promote root elongation effect to be enhancing trend with the increase of scutellarin concentration when 10-100 μ M.
Described parahormone, scutellarin concentration are brought into play maximum promotion effect when 80-100 μ M, make root ratio of elongation control group surpass more than 5 times.
The application of scutellarin in the parahormone of preparation promotion plant roots elongation.
A kind of antagonism auxin substance 2,4-D suppress the parahormone of root elongation effect, and this parahormone is unique active component with scutellarin.
Described parahormone, the root elongation of scutellarin promotes effect and 2, the mutual antagonism of root elongation depression effect of 4-D, and antagonistic effect is a dose dependent.
Described parahormone uses 2 simultaneously, and 4-D and scutellarin are 10
-9-10
-4G/L2, in the 4-D concentration range, 100 μ M scutellarins promote the effect of root elongation with 2, and the increase of 4-D concentration is reducing tendency, and antagonistic ability descends, to 10
-4G/L 2, the 4-D complete obiteration; When scutellarin concentration increased to 1000 μ M, it was 10 to high concentration
-4G/L 2, and the 4-D antagonistic ability recovers.
Scutellarin is preparing antagonism auxin substance 2, the application in the parahormone of 4-D inhibition root elongation effect.
A kind of antagonism methyl jasmonate suppresses the parahormone of root elongation effect, and this parahormone is unique active component with scutellarin.
Described parahormone, the root elongation of scutellarin promote the mutual antagonism of root elongation depression effect of effect and methyl jasmonate Me-JA, and antagonistic effect is a dose dependent.
Described parahormone uses Me-JA and scutellarin simultaneously, and 100 μ M scutellarins promote the effect of root elongation to be reducing tendency with the increase of Me-JA concentration in 1-100 μ M Me-JA concentration range, and antagonistic ability descends, to 100 μ M Me-JA complete obiterations; When scutellarin concentration when 100 μ M increase to 1000 μ M, its antagonistic ability to 1 μ M Me-JA is enhancing trend.
The application of scutellarin in the parahormone of preparation antagonism methyl jasmonate inhibition root elongation effect.
Root is the important nutrition organs of plant; It is that plant grew up in long-term the adaptation in the life process of land; Undertaking and absorb moisture and nutriment and plant and anchor at the important function on the matrix, and to be that soil moisture stress, saline and alkaline, nutrition are deficient lack and the direct effect organ of environment stress such as soil damage by disease and insect.The root elongation growth is the important stage of growth and development of plants, and various plants hormone molecule all has the effect that influences the root elongation, like the basic element of cell division, gibberellin, growth hormone, methyl jasmonate etc.Thereby the influence that scutellarin extends plant roots is to explore scutellarin one of key in application point of penetration on plant physiology.
The present invention promptly promotes the plant roots elongation, as the breakthrough first that scutellarin is used at plant field with a kind of new effect of Secondary Metabolism of Plant product scutellarin.And scutellarin promotes the effect of plant roots elongation to have the dose effect of hormonal substance, and can other two kinds known hormones with root elongation depression effect of antagonism, embodies the pattern that is similar to different hormone interactions of molecules.The present invention promotes the announcement of the parahormone effect of plant roots elongation to scutellarin; Be not merely regulating growth of plants new selection is provided; And opened up the brand-new prospect of a slice for the application of scutellarin; Promoted the Secondary Metabolism of Plant product aspect plant physiology, to bring into play biological effect, be significant.
Description of drawings:
Fig. 1-a 0-400 μ M variable concentrations scutellarin is to the facilitation of arabidopsis root elongation;
Fig. 1-b 0-400 μ M variable concentrations scutellarin is handled the long statistics of arabidopsis root, mean+SD (n=25), and * representes that there were significant differences (P＜0.05) with contrast;
Fig. 2 screens the optimum concentration that scutellarin promotes the elongation of arabidopsis root, mean+SD (n=10);
Fig. 3 scutellarin is handled the tip of a root microstructure behind the arabidopsis;
Figure 41 00 μ M scutellarin and 10
-9-10
-4G/L concentration 2,4-D is to the antagonistic effect (S: scutellarin) of arabidopsis root elongation;
Fig. 5-a 100-1000 μ M concentration scutellarin and 10
-4G/L 2, and 4-D is to the antagonistic effect (S: scutellarin) of arabidopsis root elongation;
Fig. 5-b 100-1000 μ M concentration scutellarin and 10
-4G/L 2, and 4-D is to the long statistics of root of arabidopsis root elongation antagonistic effect, mean+SD (n=15 or 16).* there were significant differences (P＜0.05) with contrast in expression.(S: scutellarin);
Figure 61 00 μ M scutellarin and 1-100 μ M concentration methyl jasmonate are to the antagonistic effect of arabidopsis root elongation;
Figure 71 00-1000 μ M concentration scutellarin and μ M concentration methyl jasmonate are to the antagonistic effect of arabidopsis root elongation.
Embodiment:
Below in conjunction with accompanying drawing, further specify essentiality content of the present invention with embodiments of the invention, but do not limit the present invention with this.
Method among the following embodiment like no specified otherwise, is conventional method.Used test material among the following embodiment like no specified otherwise, is to buy from routine biochemistry molecular agents company and obtains.
Embodiment 1:
The preparation of scutellarin:
Title: scutellarin/4-hydroxyl baicalein-7-O-glucuronide
(scutellarin/Scutellarein?4′-methyl?ether?7-glucuronide)
Molecular formula: C
21H
18O
12
Structural formula:
Preparation: short booth bitter fleabane (Erigeron breviscapus (vant) Hand Mass) pulverizing medicinal materials → 70% ethanol percolation → concentrated extracting solution → concentrate is crossed macroreticular resin → 50% ethanol elution → concentrate eluant → concentrate and is transferred pH value to 1-2 → leave standstill crystallization in 24 hours → filter crystalline substance → methanol eddy recrystallization (repeatedly) → get pure article of scutellarin with hydrochloric acid.(the short booth bitter fleabane of 1kg medicinal material makes the pure article 3g of scutellarin)
Embodiment 2:
Elongation promotes effect to scutellarin to plant roots:
One, scutellarin promotes the phenotype analytical of plant roots elongation:
(Arabidopsis thaliana ecotypeColumbia Col) is experiment material, uses the filter paper cultured method and cultivates and handle with model plant arabidopsis wild type.Pressing the preparation method among the embodiment one, is 0.01mol/L with gained scutellarin powder dissolution in being subalkaline deionized water to final concentration, dilutes respectively with deionized water then to obtain 10,40,100,400 μ M scutellarin solution.Each concentration scutellarin is added the 6cm culture dish; Every ware 3mL, 5 repetitions of each concentration (control group then is changed to deionized water with scutellarin solution, and other operations are identical); Culture dish middle berth three metafiltration paper; Seed is evenly sowed on filter paper,, be transferred to culturing room then and cultivate in the dark vernalization of 4 ℃ of refrigerators 2 days.Condition of culture is photoperiod 14h day/10h night, 22 ℃ of day temperature, and night, temperature was 19 ℃, light intensity 120 μ molm-2s-1, humidity 45%-50%.After cultivating a week, from the different culture dishes that repeat of each concentration, get seedling at random and observe root morphology down and measure root long in anatomical lens, each measurement of concetration 25 young plant and statistical average root are long, the significance of difference of application t-test verification and contrast.Experimental result proves; 10,40,100,400 μ M variable concentrations scutellarins can both significantly promote root elongation (p＜0.05); And in 10-100 μ M concentration range,, the plain concentration of second promotes that effect is enhancing trend along with increasing it; When surpassing 100 μ M its promote effect then be reduction trend (Fig. 1-a ,-b).
Two, scutellarin promotes the optimum concentration screening of plant roots elongation:
Further for confirming that scutellarin promotes the optimum concentration of root elongation; Press cultivation and processing method in the present embodiment step 1; Handle arabidopsis with 20,50,80,100,150,200 μ M concentration scutellarins respectively, and it is long press in the present embodiment step 1 method sample circuit measurement root.The result shows the maximum promotion effect of 80-100 μ M concentration scutellarin performance, and root elongation this moment average specific control group surpasses more than 5 times (Fig. 2), thereby 80-100 μ M is confirmed as the optimum concentration that scutellarin promotes the root elongation.
Three, scutellarin promotes the microexamination of root elongation:
Press cultivation and processing method in the present embodiment step 1, handle arabidopsis, and press method sampling in the present embodiment step 1 with optimum concentration 80 μ M scutellarins.With 5 μ g/ml propidium iodide (Propidium iodide; PI) respectively seedling root dyeing 5min is handled in contrast and scutellarin; Wash excess dyestuff with deionized water, carry out the microexamination of tip of a root structure and get figure with the Olympus laser confocal scanning microscope attached to the root surface.Parameter is provided with: exciting light 559nm, and emission light 580nm, object lens 40 *, sweep speed 25 μ m/s.The result shows that the scutellarin processing makes the cell of tip of a root elongation zone rise appreciably with respect to contrast, thereby makes the elongation zone comparison according to increasing (Fig. 3).Prove that thus scutellarin promotes the effect of root elongation mainly to realize through the elongation that promotes tip of a root elongation zone.
Present embodiment explanation scutellarin only needs micro-concentrations (μ M level) just can significantly promote effect to plant roots elongation performance; Its action effect strengthens with concentration in the finite concentration scope; But descend gradually again after reaching optimum concentration, these characteristics all meet the characteristic of plant hormone substance performance function.
Embodiment 3:
Scutellarin and hormone antagonism with root elongation depression effect:
One, scutellarin and auxin substance 2, the antagonistic effect that 4-D extends plant roots:
The elongation of the root of plant receives the control of multiple factor, and auxins is one of the plant hormone (Pilet and Saugy 1987) with root elongation depression effect, and the scutellarin effect of finding in its effect and embodiment 2 step 1 is opposite.Press cultivation and processing method in embodiment 2 step 1, combine 10 successively with 100 μ M scutellarins
-9-10
-4G/L 2, and 4-D handles arabidopsis, and pressing in embodiment 2 step 1 method sample circuit, to measure root long.The result finds, 10
-9-10
-4G/L 2, and in 4-D (growth hormone) concentration range, scutellarin (100 μ M) promotes the effect of root elongation with 2, and 4-D concentration raises and weakened gradually, to 10
-4G/L 2, and 4-D almost completely offsets (Fig. 4).And with 2, the 4-D concentration fixed is 10
-4G/L increases scutellarin concentration successively from 100 μ M to 1000 μ M, and arabidopsis is cultivated, handles, measured to same method.The result shows that increasing scutellarin concentration can make the trend development of the combination effect of two kinds of hormones towards the elongation of promotion root, and it promotes effect of root elongation to occur (figure) again when scutellarin increases to 1000 μ M.This explains scutellarin and 2, and elongation has antagonistic effect to 4-D to plant roots, and antagonistic effect is a dose dependent.
Two, scutellarin and methyl jasmonate are to the antagonistic effect of plant roots elongation:
Methyl jasmonate (Me-JA) is that another kind has the plant hormone (Maciejewska and Kopcewicz 2002) that suppresses root elongation effect.Press cultivation and processing method in embodiment 2 step 1, combine 1-100 μ M Me-JA to handle arabidopsis successively, and it is long press in embodiment 2 step 1 method sample circuit measurement root with 100 μ M scutellarins.The result shows that 1 μ M Me-JA can significantly disturb the root elongation of scutellarin to promote effect, and 100 μ M Me-JA then almost completely suppress the effect (Fig. 5) of scutellarin.On the other hand, be 1 μ M with the Me-JA concentration fixed, scutellarin concentration increases to 1000 μ M from 100 μ M successively, and arabidopsis is cultivated, handles, measured to same method.The result shows that the interference effect of Me-JA obviously weakens along with scutellarin concentration increases, and scutellarin promotes the effect of root elongation to recover gradually.This explanation is with 2, and 4-D is the same, and elongation also has antagonistic effect to plant roots for scutellarin and Me-JA, and antagonistic effect also is a dose dependent.
Present embodiment shows scutellarin and 2, and elongation has significant antagonism each other to two kinds of hormones of 4-D and Me-JA to plant roots, explains that scutellarin has and plant hormone 2 in the plant roots elongation process, the identical important function with Me-JA of 4-D.
In sum, scutellarin has the biological effect that promotes the plant roots elongation, and only needs μ M level concentration just can bring into play effect, and the characteristic of this extremely low concentration performance physiological effect is consistent with plant hormone substance; Secondly; Promotion effect to the root elongation increases and strengthens along with scutellarin concentration in the finite concentration scope; But then on a declining curve to the facilitation of root elongation when reaching ceiling effect continued increase concentration, the binding mode that the pattern of this performance effect and most plants hormone low concentration promote high concentration to suppress is similar; At last; Scutellarin promote the root elongation effect can with have root and extend inhibiting two plant species hormones 2; The mutual antagonism of 4-D and methyl jasmonate; And antagonistic effect is dose dependent, and antagonism rule this and the other plant hormone meets between the hormone characteristic that interacts, regulates each other.So the present invention finds: scutellarin has the parahormone effect that promotes the plant roots elongation.
Claims (16)
1. a parahormone that promotes the plant roots elongation is characterized in that containing scutellarin.
2. parahormone according to claim 1 is characterized in that it is unique active component with scutellarin.
3. parahormone according to claim 1 and 2 is characterized in that scutellarin concentration is 10-400 μ M.
4. parahormone according to claim 1 and 2 is characterized in that scutellarin concentration promotes root elongation effect to be enhancing trend with the increase of scutellarin concentration when 10-100 μ M.
5. parahormone according to claim 1 and 2 is characterized in that scutellarin concentration brings into play maximum promotion effect when 80-100 μ M.
6. parahormone according to claim 3 is characterized in that scutellarin concentration promotes root elongation effect to be enhancing trend with the increase of scutellarin concentration when 10-100 μ M.
7. parahormone according to claim 3 is characterized in that scutellarin concentration brings into play maximum promotion effect when 80-100 μ M.
8. the application of scutellarin in the parahormone of preparation promotion plant roots elongation.
9. antagonism auxin substance 2,4-D suppress the parahormone of root elongation effect, it is characterized in that this parahormone is unique active component with scutellarin.
10. parahormone according to claim 9 is characterized in that the root elongation of scutellarin promotes effect and 2, the mutual antagonism of root elongation depression effect of 4-D, and antagonistic effect is a dose dependent.
11. parahormone according to claim 9 is characterized in that using simultaneously 2,4-D and scutellarin are 10
-9-10
-4G/L 2, and in the 4-D concentration range, 100 μ M scutellarins promote the effect of root elongation with 2, and the increase of 4-D concentration is reducing tendency, and antagonistic ability descends, to 10
-4G/L 2, the 4-D complete obiteration; When scutellarin concentration increased to 1000 μ M, it was 10 to high concentration
-4G/L 2, and the 4-D antagonistic ability recovers.
12. scutellarin is preparing antagonism auxin substance 2, the application in the parahormone of 4-D inhibition root elongation effect.
13. an antagonism methyl jasmonate suppresses the parahormone of root elongation effect, it is characterized in that this parahormone is unique active component with scutellarin.
14. parahormone according to claim 13 it is characterized in that the root elongation of scutellarin promotes the root of effect and methyl jasmonate Me-JA to extend the mutual antagonism of depression effect, and antagonistic effect is a dose dependent.
15. parahormone according to claim 13; It is characterized in that using simultaneously Me-JA and scutellarin; 100 μ M scutellarins promote the effect of root elongation to be reducing tendency with the increase of Me-JA concentration in 1-100 μ M Me-JA concentration range; Antagonistic ability descends, to 100 μ MMe-JA complete obiterations; When scutellarin concentration when 100 μ M increase to 1000 μ M, its antagonistic ability to 1 μ M Me-JA is enhancing trend.
16. the application of scutellarin in the parahormone of preparation antagonism methyl jasmonate inhibition root elongation effect.
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Cited By (3)
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| CN105981720A (en) * | 2016-02-03 | 2016-10-05 | 中国科学院昆明植物研究所 | Polar auxin transport accelerant and application thereof |
| CN107365736A (en) * | 2017-08-07 | 2017-11-21 | 中国科学院昆明植物研究所 | A kind of inhibitor of plant root tip stem cell Asymmetric division regulatory pathway and its application |
| CN107460155A (en) * | 2017-08-07 | 2017-12-12 | 中国科学院昆明植物研究所 | Two kinds of accelerator of plant root tip stem cell Asymmetric division regulatory pathway and its application |
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| CN101220065A (en) * | 2008-01-24 | 2008-07-16 | 沈阳药科大学 | Novel scutellarin compounds and uses thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105981720A (en) * | 2016-02-03 | 2016-10-05 | 中国科学院昆明植物研究所 | Polar auxin transport accelerant and application thereof |
| CN107365736A (en) * | 2017-08-07 | 2017-11-21 | 中国科学院昆明植物研究所 | A kind of inhibitor of plant root tip stem cell Asymmetric division regulatory pathway and its application |
| CN107460155A (en) * | 2017-08-07 | 2017-12-12 | 中国科学院昆明植物研究所 | Two kinds of accelerator of plant root tip stem cell Asymmetric division regulatory pathway and its application |
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