CN102608266B - Ecological environment simulating system for ocean intertidal zone in climatic change background and application thereof - Google Patents

Ecological environment simulating system for ocean intertidal zone in climatic change background and application thereof Download PDF

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CN102608266B
CN102608266B CN201210054111.1A CN201210054111A CN102608266B CN 102608266 B CN102608266 B CN 102608266B CN 201210054111 A CN201210054111 A CN 201210054111A CN 102608266 B CN102608266 B CN 102608266B
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tank
hole
gas
liquid line
buffer memory
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CN102608266A (en
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付晚涛
张菊林
安宗福
苏延明
王刚
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Dalian Ocean University
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Dalian Ocean University
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Abstract

The invention discloses an ecological environment simulating system for an ocean intertidal zone in climatic change background and application thereof. The system comprises a simulation ecological system I, a gas circulating system II and a liquid circulating system III; a system bearing water tank (1) is used for structuring the simulation ecological system I; the system bearing water tank (1), a buffer water tank (2), an air bag (3) and gas pipelines (41, 42 and 43) constitute the gas circulating system II, wherein the gas pipelines are used for connecting the system bearing water tank (1), the buffer water tank (2) and the air bag (3) in series; and the system bearing water tank (1), the buffer water tank (2) and liquid pipelines (51 and 52) for connecting the system bearing water tank (1) and the buffer water tank (2) constitute the liquid circulating system III. By utilization of the ecological environment simulating system for the ocean intertidal zone in the climatic change background and a relevant method, an intertidal zone ecological environment as required can be structured repeatedly successfully under a controllable condition and can be successfully simulated under the climatic change condition, and thus, the ecological environment of the type can be further researched, developed and utilized.

Description

Climate change background Ocean intertidal zone environmental simulation system and application thereof
Technical field
The present invention relates to simulation system and the application thereof of a class special ecological environment.
Background technology
Global climate change appreciable impact terrestrial ecosystem.Coastal zone carries the earth more than 50% population, and the year GDP in the world 70% results from this, and therefore studying the impact of climate change on coastal ocean Eco-Environment System is focal issue.But climate change and the coastal ocean ecosystem are all very big yardsticks, though and climatic change trend clearly but this trend how to affect be unknown in the future producing, therefore how the impact of modeling effort climate change on coastal ocean Eco-Environment System is the problem that the mankind must solve.
Global climate change and the impact of coastal ocean ecologic environment is mainly contained: CO in air 2concentration raises and causes seawater pH value to reduce (i.e. Ocean acidification); CO in air 2concentration raise the global warming caused make ocean temperature raise, sea level rise; CO in air 2concentration raises the ozone destroyed in air, causes earth surface solar ultraviolet radiation level to raise (" marine ecology " 2009).Mesolittoral zone is in the position of forming a connecting link in the coastal ocean ecosystem, every day is in the face of high tide, ebb tide, mesolittoral zone sea life are in seawater immersion and are exposed under the alternately change of air, and therefore climate change is both complicated but must grasp on the impact of intertidal zone system.Setting up background of global climate change lower intertidal zone marine ecology simulation system, is the desirable route that human research's coastal ocean Eco-Environment System and then research Global climate change affect coastal ocean Eco-Environment System.In prior art, the impact of spininess on the single climate change factor conducts a research, and such research can not reflect the combined influence of climate change authentic and validly.The present invention is intended to for the existing present Research in this area, sets up a kind of system that can be used for simulation of global climate change background Ocean intertidal zone environment, and is applied in relevant investigation.
Summary of the invention
Climate change background Ocean intertidal zone environmental simulation system of the present invention is made up of model ecosystem I, gas-circulating system II and fluid circulation system III; System bearing tank is for building model ecosystem I; The gas piping composition gas-circulating system II of system bearing tank, buffer memory tank, air bag and the three that connects; System bearing tank, buffer memory tank and the composition of the liquid line both connection fluid circulation system III;
Described system bearing tank sidewall arranges inlet through hole, and opposing sidewalls arranges high tide level overflow through hole and low tide overflow through hole; Buffer memory tank sidewall is oppositely arranged inlet through hole and water outlet through hole;
Liquid line connected system carrying tank eases up water storage flume, and it branches into high tide level run-down pipe and low tide run-down pipe near a section of system bearing tank, is opened on high tide level overflow through hole and the low tide overflow through hole of system bearing tank sidewall respectively; Liquid line is arranged tidal level variable valve; The liquid line other end is opened on the inlet through hole of buffer memory tank sidewall; Liquid line installs peristaltic pump;
Liquid line connected system carrying tank eases up water storage flume, and the inlet through hole being opened on system bearing tank sidewall is respectively eased up the water outlet through hole of water storage flume sidewall; Liquid line installs peristaltic pump;
Gas piping is connected system carrying tank and air bag, air bag and buffer memory tank respectively, and buffer memory tank and system bearing tank; The position of the opening of gas piping on tank is all higher than the position of presetting peak level in two tanks.
The present invention provides simulation climate to change the method for background Ocean intertidal zone environmental system on the other hand, and described method uses ecologic environment simulation system disclosed in this invention, comprises the steps:
1. inject by the seawater prepared that imposes a condition in system bearing tank eases up water storage flume, connected system carrying tank, buffer memory tank and liquid line, form airtight fluid circulation system III;
2. in air bag, inject the gas according to the preparation that imposes a condition, connected system carrying tank, buffer memory tank, air bag and gas piping, form airtight gas-circulating system II;
3. open all gas valve on gas piping, make gas diffusive equilibrium in whole gas-circulating system II;
4. select to open valve or valve according to the high tide level/low tide condition of setting, and open other valves all on liquid line, open peristaltic pump, regulate its flow for setting flow, fluid circulation system III maritime interior waters is circulated;
5. according to the mesolittoral zone seawater fluctuation feature of required simulation, alternately On/Off tidal level variable valve, and adjust the flow of peristaltic pump.
Use climate change background Ocean intertidal zone environmental simulation system and the correlation method of the invention described above, the intertidal zone environment required for building successfully can be repeated under controlled condition, and can intertidal zone environment under successful analog variation weather conditions, further the research and development of this type of ecologic environment is utilized to utilize.Specifically:
(1) the present invention simulates the flood tide of mesolittoral zone, the ecosystem of ebb tide, the actual conditions of mesolittoral zone can be reflected more accurately, truly, biology as some set of mesolittoral zone growth can be exposed to when ebb tide in air, in submergence at the incoming tide, in the seawater, this does not realize in existing technology or method.
(2) impact of climate change on coastal ocean ecologic environment mainly contains CO in air 2concentration rising, seawater acidifying are (namely with CO in air 2concentration raises and the seawater pH value that causes reduces), ocean temperature rising, solar ultraviolet radiation enhancing etc.System and method of the present invention can be used for investigating the effect that this several main affecting factors acts on ocean, mesolittoral zone emulated ecological environment system simultaneously.
Accompanying drawing explanation
Accompanying drawing 2 width of the present invention, wherein:
Fig. 1 is climate change background Ocean intertidal zone environmental simulation system structural representation of the present invention;
Fig. 2 is system bearing sink structure schematic diagram.
In accompanying drawing:
1, system bearing tank; 2, buffer memory tank; 3, air bag; 4, gas piping; 5, liquid line; 6, inlet through hole i; 7, high tide level overflow through hole; 8, low tide overflow through hole; 9, inlet through hole ii; 10, water outlet through hole; 11, opening; 12, peristaltic pump; 13, Liquid valve; 131, flood tide position variable valve, 132 ebb tide position variable valve; 14, gas valve; 15, quartz plate.
Embodiment
The invention provides a kind of climate change background Ocean intertidal zone environmental simulation system, be made up of model ecosystem I, gas-circulating system II and fluid circulation system III; System bearing tank 1 is for building model ecosystem I; The gas piping 41,42,43 of system bearing tank 1, buffer memory tank 2, air bag 3 and the three that connects forms gas-circulating system II; System bearing tank 1, buffer memory tank 2 and the liquid line 51,52 both connection form fluid circulation system III;
Described system bearing tank 1 sidewall arranges inlet through hole i6, and opposing sidewalls arranges high tide level overflow through hole 7 and low tide overflow through hole 8; Buffer memory tank 2 sidewall is oppositely arranged inlet through hole ii9 and water outlet through hole 10;
Liquid line 51 connected system carrying tank 1 eases up water storage flume 2, it branches into high tide level run-down pipe 511 and low tide run-down pipe 512 near a section of system bearing tank 1, is opened on high tide level overflow through hole 7 and the low tide overflow through hole 8 of system bearing tank 1 sidewall respectively; Liquid line 511,512 is arranged tidal level variable valve 131,132; Liquid line 51 other end is opened on the inlet through hole ii9 of buffer memory tank 2 sidewall; Liquid line 51 is installed peristaltic pump 121;
Liquid line 52 connected system carrying tank 1 eases up water storage flume 2, and the inlet through hole i6 being opened on system bearing tank 1 sidewall respectively eases up the water outlet through hole 10 of water storage flume 2 sidewall; Liquid line 52 is installed peristaltic pump 122;
Gas piping 41,42,43 is connected system carrying tank 1 and air bag 3, air bag 3 and buffer memory tank 2 respectively, and buffer memory tank 2 and system bearing tank 1; The position of the opening 11 of gas piping 41,42,43 on tank 1,2 is all higher than the position of presetting peak level in two tanks.
Of the present invention one of preferred embodiment, be characterised in that described inlet through hole i6, high tide level overflow through hole 7 and the low tide overflow through hole 8 system of distance carrying distance at tank 1 end and the ratio of tank Sidewall Height are respectively 0.6 ~ 1: 1,0.62 ~ 0.65: 1,0.28 ~ 0.35: 1.Most preferably be respectively 0.6,0.62 and 0.3.
In an optimal way more of the present invention, in order to investigate the impact of ultraviolet irradiation on the target ecosystem, described system bearing tank 1 top cover arranges quartz plate 15, arranges ultraviolet irradiation device above quartz plate 15.In using, can be regulated the ultraviolet radiation entering system bearing tank 1 by different modes.One can by regulating the intensity of ultraviolet irradiation light source above quartz plate 15, the distance with quartz plate 15, it two also can arrange movable overcover in system bearing tank 1 top cover quartz plate 15 region, by this overcover mobile, regulate the size of quartz plate 15 exposed region, thus realize the adjustment of ultraviolet radiation.As optimal way, described quartz plate 15 area is 45 ~ 55% of system bearing tank 1 top cover area.
From security of system angle, avoid producing the outer impact of design because liquid/gas leaks to the constructed ecosystem, can by installing valve additional to control on pipeline.One of optimal way arranges gas valve 141,142 on described gas piping 41,42.Also on liquid line 51,52, Liquid valve 134,135 can be set near buffer memory tank 2 position further.
The present invention provides the method utilizing said system simulation climate to change background Ocean intertidal zone environmental system on the other hand, in preferred technical scheme, except building model ecosystem I and the adjustment to gas-circulating system II and fluid circulation system III, also comprise the step to the ultraviolet irradiation intensity adjustment entering system bearing tank 1, make the ultraviolet irradiation intensity being transmitted into system bearing tank 1 be setting value, usually namely investigate the UV radiation intensity in region.
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment
As shown in Figure 1: climate change background Ocean intertidal zone environmental simulation system, is made up of model ecosystem I, gas-circulating system II and fluid circulation system III;
System bearing tank 1 is rectangular parallelepiped closed container, prepared by organic glass, external dimensions length × wide ×=high 50cm × 30cm × 50cm, its sidewall arranges inlet through hole i6 apart from bottom land 30cm place, opposing sidewalls arranges high tide level overflow through hole 7 and low tide overflow through hole 8, and the two is respectively apart from bottom land 31cm and 15cm.Arrange 35cm × 21cn quartz plate 15 in the middle of system bearing tank 1 top cover, the non-quartz plate region of top cover arranges through hole 111 and 112.Above quartz plate 15, ultraviolet irradiation device is set.The present embodiment medium ultraviolet (UVR) radiation appliance is with Ultraphan 295 filter membrane (UVT 100, Digefra, Munich, Germany) uviol lamp is covered, allow the light transmission of more than wavelength 295nm, UVC is filtered, and ensures UVR (UVA and UVB) radiation.By the radiation intensity of spectral radiometer monitoring UVA and UVB.In order to the amount regulating solar ultraviolet radiation UVR to irradiate the system that enters, the measure-alike poly (methyl methacrylate) plate of quartz plate is therewith adopted to be placed on quartz plate, poly (methyl methacrylate) plate above sliding from from side to opposite side, the amount that then UVR enters system is exposed to sunlight gradually with quartz plate below and increases gradually, otherwise the amount that then UVR enters system is hidden in gradually under poly (methyl methacrylate) plate with quartz plate below and reduces gradually.
Buffer memory tank 2 external dimensions is identical with system bearing tank 1, and its sidewall is oppositely arranged inlet through hole ii9 and water outlet through hole 10; Inlet through hole ii9 is arranged close to groove top cover, and water outlet through hole 10 is arranged close to bottom land.Buffer memory tank 2 top cover arranges through hole 113 and 114.
System bearing tank 1, buffer memory tank 2 and the liquid line 51,52 both connection form fluid circulation system III; Liquid line 51 connected system carrying tank 1 eases up water storage flume 2, it branches into high tide level run-down pipe 511 and low tide run-down pipe 512 near a section of system bearing tank 1, is opened on high tide level overflow through hole 7 and the low tide overflow through hole 8 of system bearing tank 1 sidewall respectively; Liquid line 511,512 is arranged tidal level variable valve 131,132, liquid line 51,52 is arranged liquid invention 134,133 and 135, wherein valve 134 is positioned at liquid line 51 near buffer memory tank 2 pipeline section, valve 133 and 135 is positioned on liquid line 52, to ease up water storage flume 2 respectively near system bearing tank 1; Liquid line 51 other end is opened on the inlet through hole ii9 of buffer memory tank 2 sidewall; Liquid line 51 is installed peristaltic pump 121.Liquid line 52 connected system carrying tank 1 eases up water storage flume 2, and the inlet through hole i6 being opened on system bearing tank 1 sidewall respectively eases up the water outlet through hole 10 of water storage flume 2 sidewall; Liquid line 52 is installed peristaltic pump 122.Liquid-circulating flowing power source in fluid circulation system III is from peristaltic pump 121,122, and the direction of liquid stream is system bearing tank 1 → liquid line 51 → buffer memory tank 2 → liquid line 52 → system bearing tank 1.
The gas piping 41,42,43 of system bearing tank 1, buffer memory tank 2, air bag 3 and the three that connects forms gas-circulating system II; Gas piping 41 connected system carrying tank 1 is connected air bag 3 and buffer memory tank 2, gas piping 43 Connection Cache tank 2 and system bearing tank 1 with air bag 3, gas piping 42; The opening of gas piping 41,42,43 on tank 1,2 is respectively 111,112,113,114.Pipeline section near air bag 3 on gas piping 41 and 42 arranges gas valve 141 and 142 respectively.Before system starts, this two places valve is in closed condition.When after the gas being filled with setting concentration in air bag 3, connect and determine that gas-circulating system II is airtight, gas valve 141 and 142 can be opened.And reach balance everywhere in chien shih gas-circulating system II when giving sufficiently long.
CO 2the main gas used in the present invention.To the topmost factor of setting and the variable concentrations CO of gas in air bag 3 2gas.For low concentration CO 2the preparation of air, first passes through NaOH solution by air, CO in air 2react with NaOH and consume; CO will not be contained again 2air trapping in air bag; Then make it to be mixed in air bag with normal air, obtain low concentration CO 2air.For high concentration CO 2the preparation of air, by air and pure CO 2(99.9%) method mixed obtains, namely first by pure for certain volume CO 2be filled with in air bag, then pump into the normal air of certain volume.To roll after air sac sealing and place at least 12 hours to ensure that in air bag, gas fully mixes.Use CO 2mixed gas in analysis-e/or determining air bag, to determine CO in mixed gas 2concentration (± 25ppm).If CO in air bag 2concentration deviation is excessive, then pass into pure CO 2or air, to adjust CO in air bag 2concentration.
Above-mentioned ecologic environment simulation system entirety is positioned over the space of temperature-controllable, and whole system is placed on and is equipped with in the PVC Lower tank of recirculated water, and recirculated water constant-temperature water circulating device is to control water temperature.
The operation of climate change background Ocean intertidal zone environmental simulation system
The all Oceanic Samples in this part, comprise reef, sandstone, seawater and sea life and all pick up from mesolittoral zone, black stone reef marine site, Dalian.
1, the difference of the ecosystem under ecologic environment simulation system of the present invention and the ecosystem under natural conditions is first investigated.
The intertidal zone environmental system of establishing target in system bearing tank 1, the simulated target marine site of selection is surrounding sea areas, Dalian.Gather the photosynthetic organism of selection area, namely tangleweed U. pertusa (Uiva pertusa) and Irish moss (chondrus ocellatus holmes) are model organism, mesolittoral zone marine animal to be distributed widely in the marine sponge Hymeniacidon perleve (Hymeniacidon perleve) of surrounding sea areas, Dalian for model organism, and gathers the seawater, reef etc. in other target marine site.With the state of nature in biological sample collection region for control test condition.
1. in buffer memory tank 2, sand filtration seawater is injected, the height of sand filtration seawater to low tide overflow through hole 8 is injected in system bearing tank 1, then the tangleweed and sponge that gather from seashore is put into, the fresh weight of three group U. pertusa is respectively 20.5g, 21.6g and 21.8g, the fresh weight of three group Irish moss is respectively 30.6g, 31.5g and 29.7g, and three pieces of marine sponge Hymeniacidon perleve fresh weights are respectively 10.9g, 12.3g and 11.7g.Connected system carrying tank 1, buffer memory tank 2 and liquid line 51,52, forms airtight fluid circulation system III;
2. in air bag 3, inject air, connected system carrying tank 1, buffer memory tank 2, air bag 3 and gas piping 41,42,43, forms airtight gas-circulating system II; 3. open all gas valve on gas piping 41,42,43, make gas diffusive equilibrium in whole gas-circulating system II;
3. first close low tide variable valve 512, open other all valve 134,133,135 on high tide level variable valve 511 and liquid line 51,52; First stage: open peristaltic pump 121,122, regulate its flow to be 5 ~ 8ml/min, make water level in system bearing tank 1 rise gradually to simulate flood tide environment; Subordinate phase is worked as water level in system bearing tank 1 and is risen to high tide level overflow through hole 7, then regulates peristaltic pump 121 and 122 flow to carry out water level in systems stabilisation carrying tank 1; Phase III, open low tide variable valve 512, regulate peristaltic pump 121,122 flow that water level in system bearing tank 1 is declined gradually.Be down to low tide overflow through hole 8, namely complete a flood tide/ebb tide simulation.
4. open ultraviolet irradiation device, make the ultraviolet irradiation intensity being transmitted into system bearing tank 1 identical with the natural conditions of reference.
5. experimental session changes whole seawater in model ecosystem every day, from system, tangleweed is taken out after 7 days, U. pertusa fresh weight is 22.3g, 23.8g and 24.1g, and the fresh weight of Irish moss is 32.5g, 33.3g and 32.0g, and marine sponge Hymeniacidon perleve fresh weight is 11.9g, 14.0g and 13.2g.The identical experiment time, mesolittoral zone arranges experiment net cage by the sea, gather tangleweed and sponge, claim its fresh weight, three group U. pertusa are respectively 21.7g, 20.3g and 20.7g, the Irish moss of three groups is respectively 32.1g, 30.6g and 31.7g, and three pieces of marine sponge Hymeniacidon perleves are respectively 9.7g, 11.3g and 10.8g.Then be put in respectively in net cage, tangleweed with pushing down with reef, sponge to be put in the nylon wire of fastening on net cage and to add several pieces little reefs.Test after 7 days, the fresh weight of U. pertusa is 23.3g, 21.9g and 22.8g, and the fresh weight of Irish moss is 34.0g, 32.7g and 33.5g, and the fresh weight of marine sponge Hymeniacidon perleve is 11.3g, 13.0g and 12.5g.Actual mesolittoral zone and the tangleweed in simulation intertidal zone system and sponge, its fresh weight all increases; Before experiment, the biomass of reality and the tangleweed of simulating in two ecosystems and sponge is without significant difference (t-test, p < 0.02); After experiment, the biomass of reality and the tangleweed of simulating in two ecosystems and sponge is also without significant difference (t-test, p < 0.02).
More than test proof, mesolittoral zone, ocean ecologic environment simulation system comprises the mesolittoral zone sea life of the different ecological such as photosynthetic organism and marine animal position, can reflect the actual conditions of mesolittoral zone relatively accurate, truly.
2, the monofactor (CO in air that climate change causes is studied 2concentration raises) impact on mesolittoral zone marine animal.
Adopt the ecologic environment simulation system that two covers are identical.Be not placed in two cover simulation intertidal zone system averages and (controlling its ocean temperature by circulation constant temperature water-bath is 16 DEG C) in the PVC Lower tank of seawater is housed.As experimental system; Set of system connects containing CO 2concentration is the air bag of the air of 450ppm, as experimental system; Another set of system connects air bag (CO 2concentration is about 380ppm), system in contrast.Two cover systems are put into sand filtration seawater respectively and are simulated flood tide and the ebb tide of mesolittoral zone; Put into marine sponge Hymeniacidon perleve 41.5g (contradistinction system) and 43.2g (experimental system) respectively simultaneously.Experimental session, the whole sand filtration seawater in replacing every day system.Test the 7th day, in two systems, add crescent diamond pattern algae (Natzchia closterum) is respectively 200 × 10 to density 4cells/mL, distinguished micro-algae density in counting experiments system and contradistinction system after 24 hours, investigated the impact of temperature on sponge filter food ability.In contradistinction system (CO2 concentration is about 380ppm) and experimental system (CO2 concentration is 450ppm), micro-algae density is respectively 86 × 10 4cells/mL and 89 × 10 4cells/mL; Result shows, the climate change factor (atmospheric CO_2 raises---from 380ppm to 450ppm) the filter food ability of sponge is had no significant effect (p < 0.05).
As can be seen here, when atmospheric CO_2 from current about 380ppm rises to 450ppm time, the filter food ability of intertidal zone marine sponge is not almost affected (p < 0.05), also namely the Ecology Action of sponge in mesolittoral zone is had no significant effect.
2, climate change is to the influence research of mesolittoral zone marine ecosystems
In two cover simulation intertidal zone systems, set of system passes into containing CO 2concentration be 1000ppm air, put into the PVC Lower tank (controlling its ocean temperature by circulation constant temperature water-bath is 18 DEG C) that seawater is housed.Ultraphan295 filter membrane is attached on quartz plate as experimental system.Pass into air in the gas-circulating system of the another set of comparative simulation ecosystem, put into the PVC Lower tank that water temperature is 16 DEG C, quartz plate 15 covers with poly (methyl methacrylate) plate, passes into containing air (CO 2concentration is about 380ppm) air bag connect, system in contrast.Contradistinction system is identical with other condition of experimental system, namely put into sand filtration seawater and simulate flood tide and the ebb tide of mesolittoral zone, put into U. pertusa 40.2g (fresh weight, lower same) and 42.7g, Irish moss 50.6g and 52.3g, marine sponge Hymeniacidon perleve 40.5g and 43.6g respectively.Experimental system is changed whole sand filtration seawater and contains CO every day 2concentration is the air bag of 1000ppm; Contradistinction system changes sand filtration seawater and air bag every day.After experiment in 7 days terminates, take down the upper cover of simulation system, make it to be in air open state, first weigh the fresh weight of tangleweed and sponge, then sponge is put into originally respective simulation system respectively, then in experimental system and contradistinction system, add crescent diamond pattern algae (Natzchia closterum) be respectively 200 × 10 to density 4cells/mL, distinguished micro-algae density in counting experiments system and contradistinction system after 24 hours, investigated the change of sponge filter food ability, and then evaluated the impact of climate change factor pair sponge Ecology Action.In experiment, detect seawater pH value.
Result shows, and before experiment, seawater mean ph value is 8.19; Change seawater mean ph value in seawater front simulation system every day and be respectively 8.16 (contradistinction systems) and 7.80 (experimental systems).For contradistinction system, front and back (1 day time) are changed in experiment, and seawater pH value is without significant difference (p < 0.05); For experimental system, front and back (1 day) are changed in experiment, seawater pH value significant difference (p > 0.05).Show CO in air 2concentration is increased to 1000ppm, will cause the obvious acidifying of seawater.In addition, after the experiment at 7 days terminates, the fresh weight of the U. pertusa in contradistinction system, Irish moss, marine sponge Hymeniacidon perleve is respectively 43.5g, 53.7g and 43.2g, increases 8.2%, 6.1% and 6.7% respectively; The fresh weight of the U. pertusa in experimental system, Irish moss and marine sponge Hymeniacidon perleve is respectively 48.5g, 57.7g and 38.7g, increases 12.9%, 10.3% and-11.2% respectively.Result shows, the biomass of climate change factor coupling to tangleweed significantly increases, but the biomass of sponge significantly reduces.Moreover sponge filter food ability contrast experiment display, after 24 hours, in contradistinction system and experimental system, micro-algae density is respectively 85 × 10 4cells/mL and 130 × 10 4cells/mL, micro-algae density decreases 57.5% and 35.0% respectively.Result shows, under the coupling of the climate change factor, the filter food ability of sponge obviously weakens, and the Ecology Action therefore as the sponge of filter feeder also obviously weakens.
More than test proof, climate change background Ocean intertidal zone environmental simulation system constructed by the present invention successfully can repeat the intertidal zone environment required for structure under controlled condition, and can intertidal zone environment under successful analog variation weather conditions, further the research and development of this type of ecologic environment is utilized to utilize.

Claims (5)

1. the method for simulation climate change background Ocean intertidal zone environmental system, it is characterized in that using climate change background Ocean intertidal zone environmental simulation system, by airtight gas-circulating system II for model ecosystem I provides the gaseous environment of setting, by fluid circulation system III for model ecosystem I provides the water body environment of setting, by the alternately opening and closing parameter that arranges tidal level variable valve (131,132) for model ecosystem I provides the mesolittoral zone seawater fluctuation environment of setting;
Climate change background Ocean intertidal zone environmental simulation system wherein, is made up of model ecosystem I, gas-circulating system II and fluid circulation system III; System bearing tank (1) is for building model ecosystem I; Gas piping (41,42,43) the composition gas-circulating system II of system bearing tank (1), buffer memory tank (2), air bag (3) and the three that connects; System bearing tank (1), buffer memory tank (2) and the composition of the liquid line (51,52) both connection fluid circulation system III;
Described system bearing tank (1) sidewall arranges inlet through hole i (6), and opposing sidewalls arranges high tide level overflow through hole (7) and low tide overflow through hole (8); Inlet through hole i (6), high tide level overflow through hole (7) and low tide overflow through hole (8) the system of distance carrying distance at tank (1) end and the ratio of tank Sidewall Height are respectively 0.6,0.62 and 0.3;
Buffer memory tank (2) sidewall is oppositely arranged inlet through hole ii (9) and water outlet through hole (10);
The gentle water storage flume (2) of liquid line (51) connected system carrying tank (1), it branches into high tide level run-down pipe (511) and low tide run-down pipe (512) near a section of system bearing tank (1), is opened on high tide level overflow through hole (7) and the low tide overflow through hole (8) of system bearing tank (1) sidewall respectively; Liquid line (511,512) is arranged tidal level variable valve (131,132); Liquid line (51) other end is opened on the inlet through hole ii (9) of buffer memory tank (2) sidewall; Liquid line (51) is installed peristaltic pump (121);
The gentle water storage flume (2) of liquid line (52) connected system carrying tank (1), is opened on the water outlet through hole (10) of gentle water storage flume (2) sidewall of inlet through hole i (6) of system bearing tank (1) sidewall respectively; Liquid line (52) is installed peristaltic pump (122);
Gas piping (41,42,43) is connected system carrying tank (1) and air bag (3), air bag (3) and buffer memory tank (2) respectively, and buffer memory tank (2) and system bearing tank (1); The position of the opening (11) of gas piping (41,42,43) on tank (1,2) all higher than two tanks in preset the position of peak level;
Described method comprises the steps:
1. in the gentle water storage flume (2) of system bearing tank (1), inject the seawater by the preparation that imposes a condition, connected system carrying tank (1), buffer memory tank (2) and liquid line (51,52), form airtight fluid circulation system III;
2. in air bag (3), inject the gas according to the preparation that imposes a condition, connected system carrying tank (1), buffer memory tank (2), air bag (3) and gas piping (41,42,43), form airtight gas-circulating system II;
3. open all gas valve on gas piping (41,42,43), make gas diffusive equilibrium in whole gas-circulating system II;
4. select to open tidal level variable valve (131,132) according to the high tide level/low tide condition of setting, and other valves all opened on liquid line (51,52), open peristaltic pump (121,122), regulate its flow for setting flow, fluid circulation system III maritime interior waters is circulated;
5. according to the mesolittoral zone seawater fluctuation feature of required simulation, alternately On/Off tidal level variable valve (131,132), and adjust the flow of peristaltic pump (121,122).
2. method according to claim 1, is characterized in that described system bearing tank (1) top cover arranges quartz plate (15), and quartz plate (15) top arranges ultraviolet irradiation device.
3. method according to claim 2, is characterized in that described quartz plate (15) area is 45 ~ 55% of system bearing tank (1) top cover area.
4. method according to claim 1, is characterized in that described gas piping (41,42) arranges gas valve (141,142).
5. method according to claim 1, characterized by further comprising the step of ultraviolet irradiation intensity adjustment, makes the ultraviolet irradiation intensity being transmitted into system bearing tank (1) be setting value.
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