The specific embodiment:
Embodiment 1:
Mould inhibitor: 100 parts of soluble chitins, 20 parts of KIs, 20 parts of Potassiumiodates, 30 parts of calcium propionates, 3 parts of origanum oils;
Anti-oxidant: 15 parts of n-propyl gallates, 15 parts of dibutyl hydroxy toluenes, 10 parts of butylated hydroxy anisoles, 3 parts of citric acids, 3 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:2:2, three are beforehand with pulverization process respectively, and making its fineness is 0.2mm;
Wherein origanum oil obtains through following method:
With putting into supercritical extraction reactor after the pulverizing of 50g wild marjoram herb; Add 200mL75% ethanol as entrainer, the adjusting extracting pressure is 20MPa, and extraction temperature is 55 ℃; The entrainer flow is 0.02mL/min; Carbon dioxide flow is 1.2L/min, extracts and obtains the oily liquids that pale brown look has special aromatic odor after 2 hours, is origanum oil.
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 65 ℃ of following stirring and adsorbing 4 hours, standing adsorption was 25 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 1.0kg in the feed per ton.
Embodiment 2:
Mould inhibitor: 80 parts of soluble chitins, 13 parts of KIs, 14 parts of Potassiumiodates, 20 parts of calcium propionates, 2 parts of origanum oils;
Anti-oxidant: 10 parts of n-propyl gallates, 9 parts of butylated hydroxy-as, 8 parts of butylated hydroxy anisoles, 2 parts of citric acids, 2 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:1:2, three are beforehand with pulverization process respectively, and making its fineness is 0.2mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 55 ℃ of following stirring and adsorbing 3.5 hours, standing adsorption was 22 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 1.4kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 3:
Mould inhibitor: 120 parts of soluble chitins, 25 parts of KIs, 27 parts of Potassiumiodates, 40 parts of calcium propionates, 4 parts of origanum oils;
Anti-oxidant: 18 parts of n-propyl gallates, 16 parts of dibutyl hydroxy toluenes, 16 parts of butylated hydroxy anisoles, 4 parts of citric acids, 4 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:2:3, three are beforehand with pulverization process respectively, and making its fineness is 0.4mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 75 ℃ of following stirring and adsorbing 4.5 hours, standing adsorption was 28 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 0.8kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 4:
Mould inhibitor: 50 parts of soluble chitins, 10 parts of KIs, 10 parts of Potassiumiodates, 10 parts of calcium propionates, 1 part of origanum oil;
Anti-oxidant: 5 parts of n-propyl gallates, 5 parts of dibutyl hydroxy toluenes, 5 parts of butylated hydroxy anisoles, 1 part of citric acid, 1 part of inositol;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:1:1, three are beforehand with pulverization process respectively, and making its fineness is 0.1mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 50 ℃ of following stirring and adsorbing 3 hours, standing adsorption was 20 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 0.5kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 5:
Mould inhibitor: 150 parts of soluble chitins, 30 parts of KIs, 30 parts of Potassiumiodates, 50 parts of calcium propionates, 5 parts of origanum oils;
Anti-oxidant: 20 parts of n-propyl gallates, 20 parts of dibutyl hydroxy toluenes, 20 parts of butylated hydroxy anisoles, 5 parts of citric acids, 5 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:3:3, three are beforehand with pulverization process respectively, and making its fineness is 0.5mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 80 ℃ of following stirring and adsorbing 5 hours, standing adsorption was 30 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 1.5kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 6:
Mould inhibitor: 140 parts of soluble chitins, 12 parts of KIs, 12 parts of Potassiumiodates, 48 parts of calcium propionates, 2 parts of origanum oils;
Anti-oxidant: 18 parts of n-propyl gallates, 19 parts of dibutyl hydroxy toluenes, 6 parts of butylated hydroxy anisoles, 2 parts of citric acids, 4 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:1:3, three are beforehand with pulverization process respectively, and making its fineness is 0.3mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 60 ℃ of following stirring and adsorbing 5 hours, standing adsorption was 20 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 0.9kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 7:
Mould inhibitor: 45 parts of soluble chitins, 8 parts of KIs, 8 parts of Potassiumiodates, 7 parts of calcium propionates, 1 part of origanum oil;
Anti-oxidant: 3 parts of n-propyl gallates, 3 parts of dibutyl hydroxy toluenes, 4 parts of butylated hydroxy anisoles, 1 part of citric acid, 1 part of inositol;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:1:1, three are beforehand with pulverization process respectively, and making its fineness is 0.1mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 45 ℃ of following stirring and adsorbing 2.5 hours, standing adsorption was 18 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 0.4kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 8:
Mould inhibitor: 160 parts of soluble chitins, 35 parts of KIs, 35 parts of Potassiumiodates, 60 parts of calcium propionates, 8 parts of origanum oils;
Anti-oxidant: 25 parts of n-propyl gallates, 25 parts of dibutyl hydroxy toluenes, 30 parts of butylated hydroxy anisoles, 8 parts of citric acids, 8 parts of inositols;
Totally 1000 parts in mould inhibitor, anti-oxidant and carrier, the residue umber is a carrier;
Each weight ratio of constituents is a sepiolite in the carrier: peanut hull meal: defatted rice bran=1:3:4, three are beforehand with pulverization process respectively, and making its fineness is 0.5mm;
Origanum oil is sprayed at uniformly in the sepiolite of pulverization process, and 85 ℃ of following stirring and adsorbing 6 hours, standing adsorption was 35 hours again, and the sepiolite that will be adsorbed with origanum oil then mixes in proportion with other raw materials and carrier, and sieving promptly gets;
Add this additive of 1.6kg in the feed per ton.
All the other are with embodiment 1.
Embodiment 9:
Be disclosed a kind of composite mildewproof antioxidant in the patent of 97104323.X according to application number, its prescription and preparation method are following:
Mould inhibitor: DMF 100g;
Antioxidant: EMQ 100g, BHA 1g, BHT 20g;
Synergist: phosphoric acid (containing heavy more than 85%) 100g
Citric acid 100g
Disodium EDTA 1g
Carrier: white carbon 30g
The preparation of antioxidant: above-mentioned three kinds of antioxidant are poured in the beaker, be heated to 80 ℃, stir, BHA, BHT all are dissolved among the EMQ, subsequent use.
The preparation of synergist: disodium EDTA and citric acid and phosphoric acid are poured in the beaker, be heated to 80 ℃ of dissolvings, it is subsequent use to stir the back.
The mixing of material: take by weighing 100gDMF and put in the 500ml beaker, pour 30g white carbon mixing into, take by weighing again and mix in antioxidant 30g and the synergist 15g adding beaker, stir total amount 175g composite mildewproof antioxidant product.
Adding this product of 0.5g in every 1000g feed gets final product.
Embodiment 10:
Be disclosed a kind of composite synergistic antioxidant mildew-retarding agent in 99115498.3 the patent according to application number, its prescription and preparation method are following:
Take by weighing sodium Diacetate 10.5g, dimethyl fumarate 5.5g, ethoxyquinoline 4.5g, citral 10.5g, cloud essential oil 5g, sepiolite 64.0g, pulverize the back mixing and get final product.
Adding this product of 1g in every 1000g feed gets final product.
Test Example 1: anti-mold effect is investigated
Test feed is the conventional feed that ZhangZhou twins feed corporation,Ltd produces, and takes by weighing 10 groups of feeds, every group of 1000g; 10 experimental group add the mold-proof oxidation-resistant agent that makes among the embodiment 1-10 respectively; Pack behind the mixing, put in the chamber, in the condition held of 26~30 ℃ of temperature, relative humidity about 75%; The 20th heaven-made outward appearance observation first time, after this every growing state of observing mould at a distance from 5 days; The blank group is not added any mold-proof oxidation-resistant agent, other same experimental group.Experimental result is seen table 2.
Deposit to 55d and take a sample, strict press " mensuration of total number of molds in State Standard of the People's Republic of China G B/T13092-2006 feed " is measured total number of molds in each processed group feed.With each processed group feed mixing, the caking feed need be pulverized, and fully samples behind the mixing before the sampling.Experimental result is seen table 3.
Table 1 feed is in the characteristic that difference is deposited the stage
The feed state |
Criterion |
Result of determination |
Not mouldy |
The feed color is normal, can smell tangible feed fragrance |
- |
Indistinctly mouldy |
The feed color is normal, and feed fragrance is lighter, and a musty is indistinctly arranged |
+ |
Slightly mouldy |
The feed color shows slightly gloomy, can smell tangible musty |
++ |
Moderate is mouldy |
A small amount of point-like mildew is arranged, do not lump, musty is heavier |
+++ |
Seriously mouldy |
Outward appearance has tangible point-like mildew, caking, and musty is very heavy |
++++ |
Each processed group difference of table 2 is deposited the observed result in stage
Each processed group of table 3 is deposited the mould quantity to the 55d
Processed group |
Total plate count in the feed (individual/g) |
Control group |
75×10
4 |
Embodiment 1 |
16×10
4 |
Embodiment 2 |
25×10
4 |
Embodiment 3 |
38×10
4 |
Embodiment 4 |
41×10
4 |
Embodiment 5 |
49×10
4 |
Embodiment 6 |
52×10
4 |
Embodiment 7 |
62×10
4 |
Embodiment 8 |
66×10
4 |
Embodiment 9 |
54×10
4 |
Embodiment 10 |
57×10
4 |
Test Example 2: antioxidant effect is investigated
Test feed is the conventional feed that ZhangZhou twins feed corporation,Ltd produces, and takes by weighing 10 groups of feeds, every group of 1000g; 10 experimental group add the mold-proof oxidation-resistant agent that makes among the embodiment 1-10 respectively; Pack behind the mixing, put in the chamber, keeping temperature is 35-40 ℃; For guaranteeing that sample fully contacts with airborne oxygen, every day, biased sample was 2 times.Carry out the mensuration of peroxide value in 0d, 7d, 20d, 40d, 60d sampling; The blank group is not added any mold-proof oxidation-resistant agent, other same experimental group.Experimental result is seen table 4.
Table 4 oxidation resistance testing result
Test Example 3: the investigation of live pig growing state
Test and Selection ZhangZhou twins feed corporation,Ltd subordinate pig farm average weight is 660 of the health pig of (38.7 ± 2.3) kg, and is not remarkable through statistical analysis body weight difference.Be divided into 11 groups at random, every group of 6 repetitions, each repeats 10.Experimental group has been added the feed of the mold-proof oxidation-resistant agent that embodiment 1-10 makes, and live pig free choice feeding and drinking-water carry out immunity and expelling parasite by routine, and ramming material and weighing after 30 days calculates daily gain, daily ingestion amount, feedstuff-meat ratio (feed intake/weightening finish); The blank group is not added any mold-proof oxidation-resistant agent, other same experimental group.Experimental result is seen table 5.
Table 5 live pig growing state
Can find out from above-mentioned test, compare with the blank group, add the feed of the mold-proof oxidation-resistant agent that the present invention makes and compared with conventional feed that it is long to have a mildew-resistant phase, anti-mold effect is good; Peroxide value is lower, the characteristics that antioxidant effect is good; Edible live pig daily gain and the daily ingestion amount that has added the feed of the mold-proof oxidation-resistant agent that the present invention makes significantly increases, and feedstuff-meat ratio obviously descends; And the effect of the test group that embodiment 1-6 is corresponding generally is superior to the corresponding test group of embodiment 9-10, especially with the best results of embodiment 1.So the mold-proof oxidation-resistant agent that the present invention makes can significantly improve the culture efficiency of live pig, utilization is worthy to be popularized.