CN102599144A - Container suitable for liquid nitrogen container to store cells - Google Patents
Container suitable for liquid nitrogen container to store cells Download PDFInfo
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- CN102599144A CN102599144A CN2012100345900A CN201210034590A CN102599144A CN 102599144 A CN102599144 A CN 102599144A CN 2012100345900 A CN2012100345900 A CN 2012100345900A CN 201210034590 A CN201210034590 A CN 201210034590A CN 102599144 A CN102599144 A CN 102599144A
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- container
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- liquid nitrogen
- drawer box
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Abstract
The invention discloses a container suitable for a liquid nitrogen container to store cells, relating to the field of cell biology. The container comprises drawer boxes for storing cells, gaps among the drawer boxes and arms for connecting the drawer boxes, wherein each of the drawer boxes is a sealed square or rectangular container with a door and is made of a ferrite or martensite stainless steel (with magnetic property); and a cell storage space is arranged in the interior of each of the drawer boxes. According to the container, the problems of cell pollution, freezing tube explosion and the like due to penetration of liquid nitrogen into the freezing tube in the cell storage process can be avoided, and the safety of cell freezing is improved.
Description
Technical field
The present invention relates to the cell biology field, be specifically related to a kind of container that is used for liquid nitrogen container storage cell.
Background technology
Liquid nitrogen cryopreservation is one of main method of biological cell, tissue, organ preservation.Utilize frozen technology cell to be placed-196 ℃ liquid nitrogen low temperature preserve, can make cell temporarily break away from growth conditions and its cell characteristics is preserved, the recovery cell is used for experiment again in needs, and the storage life can reach 1 year to many decades.
The conventional method step of cell cryopreservation is following: at first in cell, add frozen protectant, frozen protectant generally contains the dimethyl sulfoxide (DMSO) (DMSO) or the glycerine of final concentration 5%～15%, and freezing point of solution is reduced; Get into cooling process then, the principle of cooling is that (under the slow freezing condition, ICW appears " freeze soon slowly and melt "; Reduced ice crystal formation; Thereby avoid cellular damage), the standard cooling rate is generally-1 to-2 ℃/min, when temperature is lower than-25 ℃, can quicken; After-80 ℃, can directly drop in the liquid nitrogen (196 ℃), the method (putting into 4 ℃ ,-20 ℃ ,-80 ℃ ,-196 ℃ successively) of the staged taked cooling is also arranged when not having the programmed cooling appearance.
The container class that is used in the existing liquid nitrogen container place the cell cryopreservation pipe is like a pail, and the frozen pipe that cell is housed is positioned in the pail earlier, and then pail is put into liquid nitrogen container.Because pail does not have sealing; The frozen pipe that cell is housed is fully immersed in the liquid nitrogen in depositing process; Liquid nitrogen is easier to infiltrate frozen pipe; Cause cell contamination, when frozen pipe taking-up is melted again, also possibly have certain insecurity because rapid expansion of the liquid-nitrogen boiling volume that infiltrates in the pipe cause frozen pipe blast.
Summary of the invention
For the container that solves existing liquid nitrogen container storage cell has the problem that possibility causes cell contamination, the blast of frozen pipe; The present invention provides a kind of novel container that is applicable to liquid nitrogen container storage cell, and it can avoid problems such as the cell contamination that causes because of the liquid nitrogen infiltration in the storing process, frozen pipe blast.
Technical scheme of the present invention is following:
A kind of container that is applicable to liquid nitrogen container storage cell; Comprise: put drawer box (1), the space (2) between the drawer box of cell and the arm (3) that connects the drawer box; Wherein drawer box (1) is the airtight square or the cuboid container of the band door (4) processed by ferrite (Ferrite) or martensite (Martensite) stainless steel (having magnetic), and drawer box inside is cell memory space (7).
In addition, drawer box door one side can also be equipped with the magnetic stripe (5) that seals to be opened to prevent drawer box door, can also have a support (6) of putting the cell cryopreservation pipe in the memory space and put, improves space availability ratio with what make things convenient for cell.
In order to guarantee that each drawer box fully is immersed in the liquid nitrogen, the height in the space between the drawer box is preferably between 1 centimetre to 5 centimetres.
The container that the present invention will have liquid nitrogen container storage cell now is transformed into the drawer box of closed by the pail of open type; Leave the space between the drawer box, when cell stored, the frozen pipe that cell is housed was positioned in the drawer box like this; The drawer box is fully immersed in the liquid nitrogen; The frozen pipe that cell is housed does not directly contact with liquid nitrogen, thereby avoids the pollution in the cell storage process, improves the safety of cell cryopreservation.
The present invention also can be used for putting the article such as biological tissue, organ of liquid nitrogen cryopreservation in addition, need not use support (6) when the article volume of putting is big.
The invention has the beneficial effects as follows, can avoid in the cell storing process infiltrating problems such as the cell contamination that frozen pipe causes, frozen pipe blast, the safety that has improved cell cryopreservation because of liquid nitrogen.
Description of drawings
Fig. 1: the overall appearance of one embodiment of the invention.
Fig. 2: the structure of the drawer box in the one embodiment of the invention.
Fig. 3: being used in the one embodiment of the invention put the support of cell cryopreservation pipe.
Embodiment
The present invention is simple in structure, and material is easy to get, and preparation method is obvious.
Method for using of the present invention is following: 1, cell is added frozen protectant and place special-purpose cell cryopreservation pipe; 2, the cell cryopreservation pipe is positioned on the cell cryopreservation pipe holder of the present invention (6); 3, the cell cryopreservation pipe holder is positioned in the cell memory space (7) of drawer box; 4, the present invention is positioned in the liquid nitrogen container.
When preserving the bigger article of biological tissue, organ equal-volume, also can not use the cell cryopreservation pipe holder, earlier will this tissue or organ be loaded in the airtight plastic sack or plastic casing, directly be positioned in the memory space of drawer box then.
Embodiment:
A kind of container (Fig. 1) that is applicable to liquid nitrogen container storage cell; Comprise drawer box (1), space (2) between the drawer box of putting cell and the arm (3) that connects the drawer box; Wherein drawer box (1) is the airtight cuboid container of the band door (4) processed by ferritic stainless steel, and drawer box inside is cell memory space (7).
In addition, drawer box door simultaneously is equipped with the magnetic stripe (5) that seals, and has a support (6) of putting the cell cryopreservation pipe in the memory space, and the height of the space between the drawer box (2) is 2 centimetres.
Claims (4)
1. one kind is applicable to that liquid nitrogen container stores the container of cell; It is characterized in that; Comprise: put drawer box, the space between the drawer box of cell and the arm that connects the drawer box; Wherein the drawer box is the airtight square or the cuboid container of the band door processed by ferrite or martensitic stain less steel (having magnetic), and drawer box inside is the cell memory space.
2. a kind of container that is applicable to liquid nitrogen container storage cell according to claim 1 is characterized in that drawer box door simultaneously is equipped with the magnetic stripe that seals.
3. a kind of container that is applicable to liquid nitrogen container storage cell according to claim 1 is characterized in that, also has a support of putting the cell cryopreservation pipe in the memory space.
4. a kind of container that is applicable to liquid nitrogen container storage cell according to claim 1 is characterized in that the height in the space between the drawer box is between 1 centimetre to 5 centimetres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100345900A CN102599144A (en) | 2012-02-16 | 2012-02-16 | Container suitable for liquid nitrogen container to store cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100345900A CN102599144A (en) | 2012-02-16 | 2012-02-16 | Container suitable for liquid nitrogen container to store cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102599144A true CN102599144A (en) | 2012-07-25 |
Family
ID=46517226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100345900A Pending CN102599144A (en) | 2012-02-16 | 2012-02-16 | Container suitable for liquid nitrogen container to store cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102599144A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108391658A (en) * | 2018-05-18 | 2018-08-14 | 山西正康生物科技有限公司 | A kind of stem cell storage box |
-
2012
- 2012-02-16 CN CN2012100345900A patent/CN102599144A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108391658A (en) * | 2018-05-18 | 2018-08-14 | 山西正康生物科技有限公司 | A kind of stem cell storage box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| DD01 | Delivery of document by public notice |
Addressee: Shanghai Angecon Biotechnology Co., Ltd. Document name: Notification that Application Deemed to be Withdrawn |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120725 |