CN102578160A - Artemisia capillaris extract with antiviral and antifungal activity as well as application of artemisia capillaris extract - Google Patents
Artemisia capillaris extract with antiviral and antifungal activity as well as application of artemisia capillaris extract Download PDFInfo
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Abstract
The invention relates to an artemisia capillaris extract and particularly relates to application of the artemisia capillaris extract as fungicide for controlling plant diseases caused by plant virus and plant fungi. The artemisia capillaris extract prepared by adopting an extraction method of the application has excellent control effect to plant virus diseases and plant fungi diseases.
Description
Technical field
The present invention relates to the Artemisia capillaris extract, particularly the Artemisia capillaris extract is used to prevent and treat the viral diseases of plants that caused by plant virus as bactericide or the application of the fungal diseases of plants that caused by plant epiphyte.
Technical background
Artemisia capillaris: Artemisia capillarisThunb
Latin name: Herba Artemisiae Scopariae
Another name: Artemisia capillaris, suede wormwood artemisia, horse elder generation, grandmother wormwood artemisia, wild blue wormwood artemisia
Medicinal part: herb
Phytomorph: stem is cylindrical, multi-branched, long 30～100cm, diameter 2～8mm; Surface lavender or purple have vertical stripe, by pubescence; Body is light, and matter is crisp, the section off-white color.Leaf is intensive, or comes off more; Three times pinniform drastic cracks of bottom Ye Erzhi, sliver bar shaped or slice shape, the white pubescence of the close quilt in two sides; One to two time pinniform of stem leaf is totally cleaved, and base portion is embraced stem, sliver filament shape; Capitulum is avette, and is most integrated coniform, and long 1.2～1。5mm, diameter 1～1.2mm has short stalk; 3～4 layers of phyllarys, avette, bract 3 splits; 6～10 of outer female flowers can reach 15,2～10 of internal layer hermaphrodite flowers.The achene Long Circle, yellowish-brown.Gas fragrance, mildly bitter flavor.
Main component: escoparone (Scoparone; Promptly 6; 7-dimethoxy coumarin 6; 7-Dime-thoxycoumarin), scopolactone (Scopoletin), capillarin (Capilarin) and cupreol (β-Sitosterol) also contain coumaric acid and other organic acids, like capillartemisin A (Capillartemisin A), capillartemisin B, deoxidation capillartemisin (Deoxycapillartemisin), chlorogenic acid (Chlorogenic acid), caffeic acid (Caffeic acid);
In addition; Artemisia capillaris also contains chromogen ketone and Flavonoid substances, like capillarisin (Capillarisin), arcapillin (Areapillin), different arcapillin (Isoareapillin), cirsimaritin (Cirsimauitin), genkwanin (Genkwanin), isorhamnetin (Isorhamnetin), Quercetin (Quercetin).
6,7 dimethyl coumarin structural formulas:
6,7 dimethoxy coumarins
These position marks article are tan powder, and gas is fragrant, bitter.
Medically function cures mainly: clearing away damp-heat, removing jaundice subcutaneous ulcer.Be used for the jaundice oliguria, wet sore itch; Infectious jaundice type hepatitis.
In agricultural production, use: through applicant's data-searching, Chinese invention patent 99111062 relates to a kind of mosquito-repellent incense, wherein relates to the mosquito-repellent incense that adopts Artemisia capillaris, ching-chieh, elm skin, CedarLeaves to form; Chinese invention patent 01130888,200410091088 and 201110082030 relates to desinsection, the bactericide that various plants extracts such as adopting tree-of-heaven, Artemisia capillaris is formed; But not about using the report of plant extracts aspect desinsection, sterilization of Artemisia capillaris separately, more do not use the relevant report of this extract for preventing and treating viroses of plant aspect at present.
Summary of the invention
The objective of the invention is to, provide a kind of Artemisia capillaris extract with antiviral and Fungicidally active to be used to prevent and treat the application of the pesticidal preparations of the plant disease that causes by plant virus or plant epiphyte as bactericide.
Through applicant's evidence, adopt Artemisia capillaris powder that the disclosed method for distilling of the application extracted or Artemisia capillaris fraction (being the Artemisia capillaris extract) to can be used as the application that bactericide is used to prepare the pesticidal preparations of the plant disease that control causes by plant virus or plant epiphyte.
The present invention takes to tap acrial part winter from the feverfew Artemisia capillaris, removes impurity and root, dries, and extracts the extract of processing through following two kinds of methods.
Concrete grammar is:
1, the extraction of Artemisia capillaris powder:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker;
3) Artemisia capillaris that crushes is dropped in the agitated reactor, add 10 times of amount 95% ethanol percolation soaked overnight;
4) collect percolate, filter, it is 1.10～1.15 clear cream that filtrating is evaporated to relative density at 60～65 ℃; Add 6～7 times of water yields subsequently, 0～4 ℃ of refrigeration was left standstill 24 hours; Refilter subsequently, filtrate and heated 1 hour down in 120 ℃, 0 ℃～4 ℃ refrigerations were left standstill 24 hours;
5) add the active carbon of 0.2 weight %, filter, filtrating and under 60～65 ℃, being evaporated to relative density is 1.15～1.20 clear cream, and vacuum drying below 80 ℃ promptly gets the Artemisia capillaris powder.
The said extracted etoh solvent can be used methyl alcohol, ethyl acetate or water, or two or more mixtures that are selected from ethanol, methyl alcohol, ethyl acetate and water replace.
2, the extraction of Artemisia capillaris fraction:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker;
3) Artemisia capillaris that crushes is dropped in the agitated reactor, add 10 times of water gagings, start and stir, be heated to 80 ℃, stir boiling 30 hours, auxiliary with ultrasonic;
4) product with step 3) removes by filter slag, and filtrating is under 80 ℃ of conditions, and most of water is removed in-0.09MPa decompression distillation, in still, makes 20% Artemisia capillaris fraction.
The concentration of Artemisia capillaris fraction is to control by the water yield that adds and steam, and the water that steams is heavy through the condensing and recycling meter;
It more than is the common process method for distilling.To in the application's preparation embodiment, detail below.
Except as otherwise noted, related " % " of the application all representes " percentage by weight ".
Two kinds of Artemisia capillaris extracts that the application adopts above-mentioned two kinds of methods to make, promptly Artemisia capillaris powder and Artemisia capillaris fraction have good effect on control plant virus or plant epiphyte.
The viroses of plant and control thereof are the big world difficult problems in the agricultural production.Reason one is because the kind of the viroses of plant is a lot; Known viral diseases of plants has kind more than 1000 at present; Simple in structure, vitality is extremely strong, and very easily propagates through manual work, seed, insect modes such as (aphid, thrips, aleyrodids etc.), harmfulness is only second to fungal disease.The 2nd, also medicament and product category be not seldom preferably at present human control to virus disease.The control efficiency to the viroses of plant is general mostly for domestic employed antivirotic at present, does not reach to produce actual requirement.Contain in the plant viroses of plant are worked the active substance that suppresses with passivation, through the experiment extraction, in addition the suitable formulation products that is processed into is a good scientific method.The plant anthracnose that in agricultural production, is caused by plant epiphyte, powdery mildew, rust, fusarium wilt, leaf spot, downy mildew, eqpidemic disease generation kind are very general and occurrence degree is very heavy.
Two kinds of Artemisia capillaris extracts that the application adopts above-mentioned two kinds of methods to make, promptly Artemisia capillaris powder and Artemisia capillaris fraction have good effect on control plant virus or plant epiphyte.For example aspect plant virus, all have good preventing and therapeutic action to the virus disease on the various crop such as tobacco mosaic virus disease, watermelon virus disease, tomato virus disease, pepper virus disease, cucumber virus disease, potato virus disease, custard squash virus disease, sponge gourd virus disease, pumpkin virus disease, Cruciferae Chinese cabbage virus disease, wild cabbage virus disease.Particularly good preventive effect is arranged to tobacco mosaic virus disease, watermelon virus disease.The above viroses of plant such as tobacco mosaic virus disease, watermelon virus disease there is the obvious in-vitro passivation and suppresses the effect of virus replication through experiment, the control efficiency of virus disease is all reached more than 75%.Aspect the control plant epiphyte; For example to plant anthracnose, powdery mildew, rust, fusarium wilt; Leaf spot, downy mildew and eqpidemic disease have good control efficiency, especially to downy mildew and eqpidemic disease; For example to cucumber downy mildew, cucumber eqpidemic disease, downy mildew of garpe, watermelon eqpidemic disease, wax gourd eqpidemic disease, capsicum epidemic disease, tomato blight, potato late blight, downy mildew of lichee, the lichee eqpidemic disease has good preventing and therapeutic action.Particularly cucumber downy mildew, cucumber eqpidemic disease and capsicum epidemic disease have good preventive effect.To cucumber downy mildew, cucumber eqpidemic disease, the capsicum epidemic disease control efficiency can reach more than 70% through experiment.And plant and environment are not had any residual, fool proof to people, animal and environment.Two kinds of Artemisia capillaris extracts that adopt above-mentioned two kinds of methods to make with its application; Be that the formulation products processing technology processed of Artemisia capillaris powder and Artemisia capillaris fraction is simple, cost is low, meet the developing direction of Pesticidal products research and development from now on, have a good application prospect.
Description of drawings:
Fig. 1: Artemisia capillaris fraction and emulsifier, antifreezing agent and water prepare the technological process of aqua;
Fig. 2: Artemisia capillaris powder and wetting agent, dispersant and inserts prepare the technological process of soluble powder;
Fig. 3: the technological process of Artemisia capillaris powder and auxiliary agent, adjuvant and preparing carriers water dispersible granules.
Further, the application relates to the biocide preparation that the Artemisia capillaris extract that made by above-mentioned two kinds of methods is processed
The bactericide of the various different dosage forms of processing with above-mentioned Artemisia capillaris extract, spraying prevents and treats viroses of plant fungal diseases of plants.
Agent for preventing and treating plant virus of the present invention and fungicide can be prepared into known method and be fit to any one formulation that agricultural goes up use, and reasonable formulation is aqua, wetting powder, soluble powder, water dispersible granules.Below describe with specific embodiment.Percentage all is weight percentage in all prescriptions.The processing technology of the various preparations of the present composition is prior art, can change to some extent according to different situations.
Embodiment
One, preparation embodiment
The extraction of embodiment 1, Artemisia capillaris powder:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker;
3) Artemisia capillaris that crushes is taken by weighing 400 kilograms and drop in 5000 liters of agitated reactors, add 4000 kilogram of 95% ethanol percolation soaked overnight;
4) collect percolate, filter, it is 1.10 clear cream that filtrating is evaporated to relative density at 65 ℃; Add 6 times of water yields subsequently, 0 ℃ of refrigeration was left standstill 24 hours; Refilter subsequently, filtrate and heated 1 hour down in 120 ℃, 0 ℃ of refrigeration was left standstill 24 hours;
5) add the active carbon of 0.2 weight %, filter, filtrating and under 65 ℃, being evaporated to relative density is 1.20 clear cream, and vacuum drying below 80 ℃ promptly gets the Artemisia capillaris powder.
The extraction of embodiment 2 Artemisia capillaris fraction:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker.
3) Artemisia capillaris that crushes is taken by weighing 400 kilograms and drop in 5000 liters of agitated reactors, add 4000 kg of water, start and stir, be heated to 80 ℃, stir boiling 30 hours, auxiliary with ultrasonic;
4) said mixture is removed by filter slag, filtrating is under 80～85 ℃ of conditions, and 3840 kg of water are removed in-0.09MPa decompression distillation, become 20% the Artemisia capillaris fraction aqueous solution in the still.
Chlorogenic acid is the active substance in the Artemisia capillaris; The applicant is through discover in a large number; The chlorogenic acid content as one of active component in the Artemisia capillaris extract in the foregoing description 1 and 2 (comprising Artemisia capillaris powder and Artemisia capillaris fraction) is high more; Its antiviral activity is the strongest, the therefore following content that adopts the chlorogenic acid of measuring in the above-mentioned Artemisia capillaris extract to confirm prepared Artemisia capillaris extract.
Its active component content differentiated and measured to Artemisia capillaris extract through above process makes can through following method.
The discrimination method of Artemisia capillaris extract:
(1) get these article 0.1g, add methyl alcohol 10ml, sonicated 15 minutes is filtered, and filtrating is as need testing solution.Other gets the chlorogenic acid reference substance, adds methyl alcohol and processes the solution that every 1ml contains 0.1mg, as reference substance solution.According to thin-layer chromatography test, draw each 2 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate, be solvent with the upper solution of butyl acetate-formic acid-water (7: 2.5: 2.5), launch, take out, dry, put under the ultraviolet lamp (365nm) and inspect.In the test sample chromatogram, with the corresponding position of reference substance chromatogram on, show the fluorescence spot of same color.
(2) under the assay item, in the chromatogram of record, answer tool and the identical chromatographic peak of parahydroxyacet-ophenone reference substance retention time.
Artemisia capillaris extractive content assay method:
Chlorogenic acid is according to high effective liquid chromatography for measuring.
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With acetonitrile-0.05% phosphoric acid solution (10: 90) is flowing phase; The detection wavelength is 327nm.Number of theoretical plate calculates by the chlorogenic acid peak should be not less than 10000.It is an amount of that the preparation precision of reference substance solution takes by weighing the chlorogenic acid reference substance, puts in the brown measuring bottle, adds 50% methyl alcohol and process every 1ml and contain 40µ The solution of g promptly gets.These article 0.3g is got in the preparation of need testing solution, and accurate the title decides, and puts in the brown measuring bottle of 50mL, and it is an amount of to add 50% methyl alcohol, and sonicated makes dissolving; Put coldly, add 50% methyl alcohol to scale, shake up, centrifugal, precision is measured supernatant 3ml; Put in the brown measuring bottle of 10ml, add 50% methyl alcohol, shake up, promptly get to scale.Accurate respectively reference substance solution and each 10～20 μ l of need testing solution of drawing of determination method inject liquid chromatograph, measure, and promptly get.
According to above-mentioned standard, the Artemisia capillaris extract that embodiment 1 and 2 makes calculates chlorogenic acid (C by dry product
16H
18O
9) content must not be less than 1.0% side for certified products.
Artemisia capillaris powder that embodiment 1 and 2 makes and Artemisia capillaris fraction are used as the preparation of following preparation further.
Embodiment 3:10% Artemisia capillaris aqua (is example with 1000 kilograms)
Production technology: take by weighing 500 kilograms of aqueous extracts of Artemisia capillaris (active constituent content 20%) and put in 1000 liters of agitated reactors, open and stir, be heated to 60 degrees centigrade; Add 40 kilograms of antifreezing agent propane diols then respectively, 100 kilograms of emulsifier op-10s, 360 kilograms in water; 60 degrees centigrade stir 1 hour after; Cool to normal temperature, detect, qualified back packing warehouse-in.Its concrete technological process is referring to Fig. 1.
Embodiment 4:40% oriental wormwood artemisin wetting powder (is example with 1000 kilograms)
Production technology: successively 400 kilograms of 400 kilograms of inserts precipitated calcium carbonates, 100 kilograms of anticaking agent white carbons, 10 kilograms of suspending agent aluminium-magnesium silicates, 400 kilograms of Artemisia capillaris powders, 60 kilograms of dispersant sodium lignin sulfonates and 30 kilograms of wetting agent BX are dropped in 1000 liters the preceding blending tank; Stirred 30 minutes; Through comminution by gas stream to more than 325 orders; Material after the pulverizing stirred 1 hour at 1000 liters back blending tank, sample analysis, qualified back packing warehouse-in.
Embodiment 5:20% oriental wormwood artemisin soluble powder (is example with 1000 kilograms)
Production technology is with embodiment 4, and its concrete technological process is referring to Fig. 2.
Embodiment 6:60% oriental wormwood artemisin water dispersible granules (is example with 1000 kilograms)
Production technology: successively 240 kilograms of inserts kaolin, 50 kilograms of disintegrant ammonium sulfate, 600 kilograms of Artemisia capillaris powders, 70 kilograms of dispersants 2500 and 40 kilograms of wetting agents 4896 are dropped in 1000 liters the preceding blending tank, stirred 30 minutes, through comminution by gas stream to more than 500 orders; Material after the pulverizing stirred 1 hour at 1000 liters back blending tank; Sample analysis drops into kneader with material after qualified, adds suitable quantity of water and mediates; Dry after the comminutor granulation; Need particle size with sifting out, inappropriate particle is mediated granulation again, the qualified back of suitable grading analysis packing warehouse-in.
Embodiment 7:1% oriental wormwood artemisin aqua (is example with 1000 kilograms)
Production technology is with embodiment 3
Embodiment 8:30% oriental wormwood artemisin wetting powder (is example with 1000 kilograms)
Production technology is with embodiment 4
Embodiment 9:50% oriental wormwood artemisin soluble powder (is example with 1000 kilograms)
Production technology is with embodiment 4
Embodiment 10:5% oriental wormwood artemisin water dispersible granules (is example with 1000 kilograms)
The concrete technological process of production is referring to Fig. 3.
Below through concrete biologicall test experiment Artemisia capillaris extract and preparation thereof that the application relates to are set forth viroses of plant control efficiency.
For making things convenient for the following Artemisia capillaris extract of appellation to replace with YCH.
Two, indoor biological activity determination embodiment
Employed Artemisia capillaris extract (being YCH) refers to embodiment 1 and 2 prepared Artemisia capillaris powder and Artemisia capillaris fraction among the application's biological activity determination embodiment.
1, the Artemisia capillaris extract is measured the result to tobacco mosaic virus disease (TMV) inactivation in vitro
This embodiment adopts prepared Artemisia capillaris powder among the above-mentioned preparation embodiment 1; Adopt withered spot method, measured the inactivation in vitro of 3 kinds of variable concentrations Artemisia capillaris extracts TMV.The contrast medicament is selected for use, 20% Moroxydine Hydrochloride copper acetate wetting powder, and Qiqihar, Heilongjiang Province chemical industry Co., Ltd produces, and is commercially available, measures the result and sees table 1.
Can find out that from table 1 these 3 kinds of variable concentrations Artemisia capillaris extracts all show certain passivation to TMV.The passivation effect of processing 3 is best, reaches 76.28%, is higher than contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powder.The Artemisia capillaris extract shows time effect to the TMV passivation, and with the prolongation of passivation time, passivation effect strengthens gradually.Behind passivation 0.5h, 1h, the 2h, its passivation effect is respectively 57.06%, 65.32% and 76.28%.The passivation effect that all is higher than contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powder under the same terms.
The YCH of table 13 kind of variable concentrations measures the result to the inactivation in vitro of TMV
Annotate: host material is a Nicotiana glutinosa: the right half of chemicals treatment that is, the left half of contrast that is.The mean value that data bit repeats for 3 times in the table
2, TMV is just infected inhibitory action and measure the result
This embodiment adopts prepared Artemisia capillaris fraction among the above-mentioned preparation embodiment 2; The inhibitory action that the Artemisia capillaris extract that adopts withered spot method to measure 3 kinds of variable concentrations just infects TMV, the result sees table 2, can find out from table 2, the Artemisia capillaris extract of 3 kinds of variable concentrations all has the obvious suppression effect to TMV.Inoculate behind dispenser 24h, the 48h, it suppresses to TMV that effect is respectively 47.37%, 60.06%, 54.88%68.73% and 65.28%, 70.42% under 50 times, 100 times, 200 multiple doses.Its inhibition effect is compared with 20% Moroxydine Hydrochloride copper acetate wetting powder and is close to or higher than the contrast drug effect.
The Artemisia capillaris extract of table 23 kind of variable concentrations just infects inhibitory action to TMV and measures the result
Annotate: host material is a Nicotiana glutinosa: the right half of chemicals treatment that is, the left half of contrast that is.The mean value that data bit repeats for 3 times in the table
3, to the mensuration result of TMV inhibited proliferation
This embodiment adopts prepared Artemisia capillaris powder among the above-mentioned preparation embodiment 1; Adopt Artemisia capillaris extract that withered spot method measured 3 kinds of different disposal concentration to the effect of TMV inhibition of proliferation, the result sees table
3
Can find out that from table 3 the Artemisia capillaris extract that these 3 kinds of variable concentrations are handled all has certain inhibitory action to TMV propagation.Its inhibiting rate of handling 150 times of dilutions is suitable with 500 times of treatment effects of 20% Moroxydine Hydrochloride copper acetate wetting powder, handles
2And processing
3To compare according to medicament high to the TMV inhibited proliferation.
The Artemisia capillaris extract that table 33 kind of variable concentrations is handled is measured the result to the TMV inhibited proliferation
Annotate: host material is a Nicotiana glutinosa: the right half of chemicals treatment that is, the left half of contrast that is.The mean value that data bit repeats for 3 times in the table
4, indoor control tobacco mosaic virus disease result of the test
The Artemisia capillaris extract is measured the result to the tobacco virus preventive and therapeutic effect and is seen table 4.
Can find out that from table the different application methods of Artemisia capillaris extract all have certain preventive and therapeutic effect to tobacco virus.Wherein the control efficiency of 400 times of processing of Artemisia capillaris extract is the highest, and the control efficiency of 14d and 21d reaches 74.32% and 80.31% respectively, apparently higher than the contrast medicament control efficiency of 20% Moroxydine Hydrochloride copper acetate wetting powder same period.
Table 4 Artemisia capillaris extract is measured the result to the tobacco virus preventive and therapeutic effect
Annotate: every processing 3 repeats, and data are the mean value of 3 repetitions in the table.Lowercase P＜0.05, Duncan formula duncan's new multiple range method.
Three, field control effectiveness test
1, Artemisia capillaris extract 10% aqua control watermelon virus disease field control effectiveness test
This embodiment adopts prepared Artemisia capillaris fraction among the above-mentioned preparation embodiment 3; Each is handled the control efficiency of watermelon virus disease is seen table 5.Each is handled and contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powder all has good preventive effect to the watermelon virus disease from table, can to find out Artemisia capillaris extract 10% aqua, and each preventive effect of handling all with contrast quite or be higher than contrast.The highest preventive effect can reach more than 80%.
Table 5 Artemisia capillaris extract 10% aqua is measured the result to the effect of watermelon virus disease control
Annotate: every processing 3 repeats, and data are the mean value of 3 repetitions in the table.Lowercase P＜0.05, Duncan formula duncan's new multiple range method.
2, Artemisia capillaris extract 10% aqua control tomato virus disease field control effectiveness test
This embodiment adopts prepared Artemisia capillaris fraction among the above-mentioned preparation embodiment 3; Each is handled the control efficiency of tomato virus disease is seen table 6.Each is handled and contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powder all has good preventive effect to tomato virus disease from table, can to find out Artemisia capillaris extract 10% aqua, and each preventive effect of handling all with contrast quite or be higher than contrast.The highest preventive effect can reach more than 80%.
Table 6 Artemisia capillaris extract 10% aqua is measured the result to the tomato virus disease preventive and therapeutic effect
Annotate: every processing 3 repeats, and data are the mean value of 3 repetitions in the table.Lowercase P＜0.05, Duncan formula duncan's new multiple range method.
3, Artemisia capillaris extract 10% aqua control cucumber downy mildew, the sick field control effectiveness test of eqpidemic disease
This embodiment adopts prepared Artemisia capillaris fraction among the above-mentioned preparation embodiment 3; Each is handled the control efficiency of cucumber downy mildew, cucumber eqpidemic disease is seen table 7.Can find out that from table each processing of Artemisia capillaris extract 10% aqua and contrast medicament 50% dimethomorph wetting powder all have good preventive effect to cucumber downy mildew, cucumber eqpidemic disease, and each preventive effect of handling is sprayed a little less than 1500 times of liquid of contrast medicament.But average preventive effect is all more than 70%.
Table 7 Artemisia capillaris extract 10% aqua is measured the result to cucumber downy mildew, cucumber epidemic prevention and control effect
Annotate: every processing 3 repeats, and data are the mean value of 3 repetitions in the table.Lowercase P＜0.05, Duncan formula duncan's new multiple range method.
4, Artemisia capillaris extract 10% aqua control capsicum epidemic disease field control effectiveness test
This embodiment adopts prepared Artemisia capillaris extract 10% aqua among the above-mentioned preparation embodiment 3; Each is handled the control efficiency of capsicum epidemic disease is seen table 8.Can find out that from table each processing of Artemisia capillaris extract 10% aqua and contrast medicament 58% metalaxyl manganese-zinc wettable powder all have good preventive effect to capsicum epidemic disease; And each preventive effect of handling is all suitable with contrast 1500 times of liquid sprayings of medicament preventive effect, and average preventive effect is all more than 75%.
Table 8 Artemisia capillaris extract 10% aqua is measured the result to the capsicum epidemic disease preventive and therapeutic effect
Annotate: every processing 3 repeats, and data are the mean value of 3 repetitions in the table.Lowercase P＜0.05, Duncan formula duncan's new multiple range method.
Show that through above research the Artemisia capillaris extract has certain inactivation in vitro to TMV, TMV is just contaminated and to duplicate the inhibition of proliferation effect obvious.Indoor pot experiment shows that the Artemisia capillaris extract has good control efficiency to tobacco mosaic virus disease (TMV).Wherein, the control efficiency of 400 times of processing of Artemisia capillaris extract is the highest, and the control efficiency of 14d and 21d reaches 74.32% and 80.31% respectively, apparently higher than the contrast medicament control efficiency of 20% Moroxydine Hydrochloride copper acetate wetting powder same period.
Field control effectiveness test shows that the Artemisia capillaris extract all has good control efficiency to watermelon, tomato virus disease.Can reach more than 80% in 400 times of sprayings of 10% Artemisia capillaris extract aqua preventive effect.Cucumber downy mildew, eqpidemic disease, capsicum epidemic disease are also had good control efficiency, and 10% Artemisia capillaris extract aqua 600-800 doubly sprays preventive effect all more than 70%.Hence one can see that, and the Artemisia capillaris extract has good inhibition effect to the viroses of plant and downy mildew, eqpidemic disease.By the Artemisia capillaris extract be various formulation bactericide that main active ingredient is processed by technical scheme of the present invention in agricultural production, be used to prevent and treat the viroses of plant and and by fungus-caused plant anthracnose, powdery mildew, rust, fusarium wilt, leaf spot particularly downy mildew, eqpidemic disease all can reach desirable effect.
Artemisia capillaris extract and application thereof with antiviral and Fungicidally active of the present invention is described through concrete instance; Those skilled in the art can use for reference content of the present invention; Links such as appropriate change raw material, process conditions realize corresponding other purpose; Its relevant change does not all break away from content of the present invention, and all are similarly replaced and change will become apparent to those skilled in the art that all to be regarded as and is included within the scope of the present invention.
Claims (9)
1. one kind has antiviral and Artemisia capillaris extract bactericidal activity, and it adopts following method to make:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker;
3) Artemisia capillaris that crushes is dropped in the agitated reactor, add 10 times of amount 95% ethanol percolation soaked overnight;
4) collect percolate, filter, it is 1.10～1.15 clear cream that filtrating is evaporated to relative density at 60～65 ℃; Add 6～7 times of water yields subsequently, 0～4 ℃ of refrigeration was left standstill 24 hours; Refilter subsequently, filtrate and heated 1 hour down in 120 ℃, 0 ℃～4 ℃ refrigerations were left standstill 24 hours;
5) add the active carbon of 0.2 weight %, filter, filtrating and under 60～65 ℃, being evaporated to relative density is 1.15～1.20 clear cream, and vacuum drying below 80 ℃ promptly gets the Artemisia capillaris powder.
2. according to the Artemisia capillaris extract of claim 1, wherein extract etoh solvent and can use methyl alcohol, ethyl acetate or water, or two or more mixtures that are selected from ethanol, methyl alcohol, ethyl acetate and water replace.
3. one kind has antiviral and Artemisia capillaris extract bactericidal activity, and it adopts following method to make:
1) the Artemisia capillaris acrial part (complete stool comprises cane, leaf, seed) after will tapping winter is removed impurity and root, dries;
2) Artemisia capillaris that dries is crushed to more than 30 orders with cracker;
3) Artemisia capillaris that crushes is dropped in the agitated reactor, add 10 times of water gagings, start and stir, be heated to 80 ℃, stir boiling 30 hours, auxiliary with ultrasonic;
4) product with step 3) removes by filter slag, and filtrating is under 80 ℃ of conditions, and most of water is removed in-0.09MPa decompression distillation, in still, makes 20% Artemisia capillaris fraction.
4. the arbitrary described Artemisia capillaris extract of the claim 1-3 purposes that is used to prevent and treat viral diseases of plants and fungal diseases of plants.
5. according to the purposes of claim 4, viral diseases of plants wherein is tobacco mosaic virus disease, watermelon virus disease, tomato virus disease, pepper virus disease, cucumber virus disease, potato virus disease, custard squash virus disease, sponge gourd virus disease, pumpkin virus disease, Chinese cabbage virus disease or wild cabbage virus disease.
6. according to the purposes of claim 4, viral diseases of plants wherein is tobacco mosaic virus disease, watermelon virus disease or tomato virus disease.
7. according to the purposes of claim 4, fungal diseases of plants wherein is anthracnose, powdery mildew, rust, fusarium wilt, leaf spot, downy mildew or eqpidemic disease.
8. according to the purposes of claim 7, fungal diseases of plants wherein is: cucumber downy mildew, cucumber eqpidemic disease, downy mildew of garpe, watermelon eqpidemic disease, wax gourd eqpidemic disease, capsicum epidemic disease, tomato blight, potato late blight, downy mildew of lichee or lichee eqpidemic disease.
9. according to the purposes of claim 7, fungal diseases of plants wherein is cucumber downy mildew, cucumber eqpidemic disease or capsicum epidemic disease.
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