CN102573512A - Food additive containing eugenol, cinnamaldehyde and an alliaceous extract - Google Patents
Food additive containing eugenol, cinnamaldehyde and an alliaceous extract Download PDFInfo
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- CN102573512A CN102573512A CN2010800313218A CN201080031321A CN102573512A CN 102573512 A CN102573512 A CN 102573512A CN 2010800313218 A CN2010800313218 A CN 2010800313218A CN 201080031321 A CN201080031321 A CN 201080031321A CN 102573512 A CN102573512 A CN 102573512A
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- Prior art keywords
- supplements
- methane
- formula
- ruminant
- extract
- Prior art date
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- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 title claims abstract description 40
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 239000005770 Eugenol Substances 0.000 title claims abstract description 20
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 229960002217 eugenol Drugs 0.000 title claims abstract description 20
- 239000000284 extract Substances 0.000 title claims abstract description 16
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 title abstract description 4
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 title abstract description 3
- 229940117916 cinnamic aldehyde Drugs 0.000 title abstract description 3
- 239000002778 food additive Substances 0.000 title abstract 3
- 235000013373 food additive Nutrition 0.000 title abstract 3
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 70
- 150000001875 compounds Chemical class 0.000 claims abstract description 19
- 241000282849 Ruminantia Species 0.000 claims abstract description 16
- 150000002898 organic sulfur compounds Chemical class 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims abstract description 4
- 239000012298 atmosphere Substances 0.000 claims abstract description 3
- 239000013589 supplement Substances 0.000 claims description 50
- 241000234282 Allium Species 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 19
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 claims description 17
- WBYWAXJHAXSJNI-SREVYHEPSA-N Cinnamic acid Chemical compound OC(=O)\C=C/C1=CC=CC=C1 WBYWAXJHAXSJNI-SREVYHEPSA-N 0.000 claims description 16
- 229930016911 cinnamic acid Natural products 0.000 claims description 16
- 235000013985 cinnamic acid Nutrition 0.000 claims description 16
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 claims description 16
- 235000002732 Allium cepa var. cepa Nutrition 0.000 claims description 15
- 240000002234 Allium sativum Species 0.000 claims description 13
- 239000006000 Garlic extract Substances 0.000 claims description 11
- 235000004611 garlic Nutrition 0.000 claims description 11
- 235000020706 garlic extract Nutrition 0.000 claims description 11
- 241000196324 Embryophyta Species 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 7
- 125000000217 alkyl group Chemical group 0.000 claims description 5
- 241000123646 Allioideae Species 0.000 claims description 4
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- XOIQMTLWECTKJL-HXPDMXKUSA-M sodium;(3r,4s)-4-[(2s,5r,7s,8r,9s)-2-[(2r,5s)-5-ethyl-5-[(2r,3s,5r)-5-[(2s,3s,5r,6r)-6-hydroxy-6-(hydroxymethyl)-3,5-dimethyloxan-2-yl]-3-methyloxolan-2-yl]oxolan-2-yl]-7-hydroxy-2,8-dimethyl-1,10-dioxaspiro[4.5]decan-9-yl]-3-methoxy-2-methylpentanoate Chemical compound [Na+].C([C@@](O1)(C)[C@H]2CC[C@@](O2)(CC)[C@H]2[C@H](C[C@@H](O2)[C@@H]2[C@H](C[C@@H](C)[C@](O)(CO)O2)C)C)C[C@@]21C[C@H](O)[C@@H](C)[C@@H]([C@@H](C)[C@@H](OC)C(C)C([O-])=O)O2 XOIQMTLWECTKJL-HXPDMXKUSA-M 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical group CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims 2
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
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- 229950008882 polysorbate Drugs 0.000 description 1
- 239000005077 polysulfide Substances 0.000 description 1
- 229920001021 polysulfide Polymers 0.000 description 1
- 150000008117 polysulfides Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940083466 soybean lecithin Drugs 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- 230000029305 taxis Effects 0.000 description 1
- WBYWAXJHAXSJNI-VOTSOKGWSA-N trans-cinnamic acid Chemical compound OC(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-N 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
Abstract
The invention relates to a food additive including: 30 to 45 % of eugenol; 30 to 45 % of cinnamaldehyde; 10 to 40 % of extract of a plant belonging to the alliaceous family; the remainder up to 100 % optionally comprising other compounds, in particular, organosulphur compounds. Such an additive can be added to the feed for certain animals, in particular bovines, with a view to reducing the methane production thereof. The invention also relates to a method for reducing methane emissions into the earth's atmosphere, which includes the following steps: adding a food additive according to the invention to the food ration of a ruminant; and feeding the ruminant the obtained food ration.
Description
Technical field
The present invention relates to comprise the supplements of eugenol (eugenol), cinnamic acid (cinnamaldehyde) and green onion section (Alliaceae) extract with special ratios.This additive can be added into particular animals, the feed of bovine (bovines) especially, produce methane to reduce it.
Background technology
Be well known that now domestic animal produces more greenhouse gases than vehicle.
Especially think that ruminant is the reason of 18% discharge of methane; Wherein according to the expert of Giec (inter-governmental climate change expert group (Intergovernmental Group of Experts on Climate Change)), the greenhouse effects of said methane are stronger 23 times than the greenhouse effects of carbon dioxide.
According to Argentinian scientist, the ox of about 550kg is discharged the gas between 800 and 1000 liters through belch (eructation) basically every day, mainly is methane.
The result is the main constraint that the protection environment becomes agricultural, and some American States are considering that cow is produced methane more and more unkindly levies taxes.
Carried out multiple trial and produced methane to reduce ox.
Some antibiotic for example has been used for feed.This scheme is just day by day abandoned; On the one hand; Because be except that cost, these antibiotic have following shortcoming, and promptly the most of the time is the supervised entities of authority; And on the other hand, because consumption " natural " product that this and consumer increase or even the product demand that obtains through organic agriculture at a distance of farther.
Recently; INRA (French national agricultural research institute (National Institute for Agronomic Research)) experiment centre shows; In the Multitest of implementing to cow, 6% lipid that picked-up obtains from linseed reduces these animals by 27 to 37% methane and produces.
With reference to " extrude the corresponding cow cud of linseed level methane with raising meals property and generate (Rumen methanogenesis of dairy cows in response to increasing levels of dietary extruded linseeds) "; Second international energy and protein metabolism and nutrition conference collection of thesis (2nd International Symposium on Energy and Protein Metabolism and Nutrition); 9-13 day in September, 2007; France; The Vichy, the 609-610 page or leaf.
Also with reference to " with feed with natural linseed, the linseed of extruding or linseed oil corresponding methane output of cow and ration digestibility (Methane output and diet digestibility in response to feeding dairy cows with crude linseed; extruded linseed or linseed oil) "; Animal science magazine (Journal ofAnimal Science), doi:10.2527/jas/2007-0774.
International patent application no WO 03/094628 discloses eugenol and has reduced the purposes that methane produces (especially with reference to the 2nd page, latter end).
The generation of people's such as Macheboeuf article report methane reduces because of cinnamic acid; This article comes across animal feed science and technology (Animal Feed Science and Technology) summary; The 145th volume; The the 1st to 4 phase, in August, 2008, (especially see Fig. 2 of the 342nd page of bottom) in the 335th to 350 page (XP002575075).
Green onion section derivative is used in international patent application no WO 2008/0377827 instruction, and especially PTS/PTSO causes fermentation gas in the ruminant, the especially discharging minimizing (with reference to the 5th page, the 3rd section) of methyl alcohol.
For reducing, obtain simple and inexpensive and can easy-to-use scheme will be desirable by animal and partly by the discharge of methane of Niu Yinqi.In addition, also should all use this scheme in developed country with in impoverished nation, the described developing country of some of them like India, Brazil and Argentinian, has the domestic animal and thereby the significantly effect of performance aspect the generation of greenhouse gases of huge amount.
Summary of the invention
Inventor of the present invention has developed supplements, and it is a characteristic features with the extract that comprises eugenol, cinnamic acid and belong to the plant of green onion section, and these compositions exist with special ratios.
The inventor observes; The feed allocation quantity (feed ration) that adds this additive to certain ruminant causes the methane generation of this ruminant to significantly reduce; And do not influence the generation of volatile fatty acid in the cud significantly; These acid are important, because they serve as the energy of said animal metabolism.
With regard to the generation of methane, in some cases, it stands the minimizing until 25%.
Description of drawings
Now, other features and advantages of the present invention will be described in detail in follow-up and disclosure by description of drawings, said accompanying drawing representative:
-Fig. 1: show that methane produces the ternary diagram of curve as the function of the supplements concentration of eugenol, cinnamic acid and allium (Allium) plant extracts;
-Fig. 2: show that volatile fatty acid produces the ternary diagram of curve as the function of the supplements concentration of eugenol, cinnamic acid and allium (Allium) plant extracts;
-Fig. 3: show every kind of processing to test, unit is the figure of average every ox methane every day generation of gram;
-Fig. 4: every kind of processing to test is described, unit is the figure of rumen fluid concentration of the volatile fatty acid of mM/L; And
-Fig. 5 to 7: show every kind of processing, the figure of acetic acid esters, malonate and butyrate percentage in the middle of the volatile fatty acid to test.
The specific embodiment
The eugenol that uses in the supplements of the present invention is the compound of knowing that is called 4-pi-allyl-2-metoxyphenol, has empirical formula C
10H
12O
2
Cinnamic acid (or cinnaldehydrum (cinnamic aldehyde)) is also known.It is to have empirical formula C
9H
8The 3-phenylacrolein of O (trans-cinnamic acid).
According to the present invention, this supplements comprises the extract of plant at least, and wherein said plant belongs to green onion section (Alliaceae) (classifying according to the APG system) and especially belongs to the allium (Allium) as the member of this section.
The most well-known plant that belongs to allium is leek (leek) (blueleek (Allium ampeloprasum)), onion (onion) (onion (Allium cepaL.)), cultivation garlic (cultivated garlic) (garlic (Allium sativumL.)), Hu green onion (shallot) (fiery green onion (Allium ascalonicum)), shallot (Welsh onion) (shallot (Allium fistulosumL.)) and chives (chive) (northern green onion (Allium schoenoprasumL.)).
According to the present invention, preferably use the extract of cultivation garlic (garlic (Allium sativumL.)).This extract is the extract of garlic bulb (garlic bulb) normally.
This garlic extract can contain dialkyl group thiosulfinate and/or at least a dialkyl group thiosulfonates.
These compounds are corresponding to following formula F:
R-SO
a-S-R′
Wherein:
R and R ' are identical or different, represent alkyl separately; And
A is 1 or 2.
Said alkyl R and R ' preferably comprise 1 to 5 carbon atom.
In addition, more specifically use propyl group (n-pro-pyl usually) as R base and R ' base.
Inner at identical formula F compound, said R base is preferred identical with R ' base.
For the dialkyl group thiosulfinate, in the F formula, a is 1.
For the dialkyl group thiosulfonates, a is 2.
As the compound of formula F, advantageously use sulfo-sulfinic acid two (n-pro-pyl) ester (being called PTS hereinafter) and/or thiosulfonic acid two (n-pro-pyl) ester (being called PTSO hereinafter).
Can obtain PTSO through decomposing (or oxidation) PTS.
Generation through extract producing PTS and PTSO is especially at patent application EP-A1-1721534, and the 15th page the 34th is walked to description in the 16th page of the 26th row.
Thiosulfinate also can obtain according to the method for describing among the patent application FR-A-2813884.
Yet PTS and PTSO necessarily do not obtain from green onion section (Alliaceae).They can certainly produce through synthetic.
Therefore the present invention also relates to supplements, and it comprises:
-30 to 45% eugenol,
-30 to 45% cinnamic acid, and
-10 to 40% at least a compound F 17-hydroxy-corticosterone;
-randomly comprise other compounds, especially organosulfur compound remainder until 100%.
Certainly, supplements of the present invention can comprise the mixture of following material with any ratio:
-at least two kinds of dialkyl group thiosulfinates;
-at least two kinds of dialkyl group thiosulfonates;
-at least a dialkyl group thiosulfinate and at least a dialkyl group thiosulfonates.
According to another embodiment of the invention, the compound of formula F can with at least a other compounds, preferably with sulphur compound, the sulphur compound combination that especially obtains from allium (Allium) plant.This other sulphur compound for example can be allicin (allicin), sulfide or polysulfide, and disulphide especially is like allyl disulfide (allyl disulfide).
These compounds can be the solution forms in the solvent (like glycerine, propane diols) or use the aqueous emulsion form of polysorbate as emulsifying agent.
Supplements of the present invention can contain other compounds well known to those skilled in the art extraly, as hydrogenated vegetable oil, maltodextrin D.E.6,9,18.5, soybean lecithin, gelatin, cyclodextrin, pectin, guar gum, dextrose, maltose, gum arabic, plug take nurse (sepifilm), hydroxypropyl methylcellulose, methylcellulose, ethyl cellulose, ethyl-methyl methylcellulose, Quillaia saponaria (quillaja) extract, polyoxyethylene (20) anhydrosorbitol acid anhydride monolaurate (polyoxyethylene (20) sorbitan monolaurate), polyoxyethylene (20) anhydrosorbitol acid anhydride monoleate, polyoxyethylene (20) anhydrosorbitol acid anhydride monostearate, polyoxyethylene (20) anhydrosorbitol acid anhydride monopalmitate, sorbitol laurate, sorbitol monooleate, D-sorbite monostearate, with following acid: the edible fatty acid monoglyceride and the diglyceride of acetate, lactic acid, citric acid, tartaric acid and single acetyl/diacetyl tartrateization.
According to favourable embodiment of the present invention, this supplements is made up of following material:
-30 to 45% eugenol,
-30 to 45% cinnamic acid and
-10 to 40% garlic extract;
-percentage summation is 100%.
In addition, said garlic extractive content is preferably between 15 and 35%, more specifically between 24 and 26%.
Can be prepared as follows supplements of the present invention, promptly through mixing eugenol, cinnamic acid, garlic extract and other the optional compositions that are in desirable ratio, until obtaining uniform mixture with any order.
Can use conventional galenical technology (galenic technique), like spraying cooling, aggegation, gelatinization, balling-up (prilling) and sprinkling in the fluidization air bed, suspension and granulation (granulation).
Supplements of the present invention is in usually by having the form of powder that following particle is formed generally, and said particle has can be from 90 μ m (micron) to 1000 mu m ranges, the size of 200 μ m and 500 mu m ranges especially.
These particles can randomly be used and be intended to cover the garlic smell and/or promote the outer coatings layer of food consumption to coat fully through stimulation animal appetite.This dressing can be a kind of dressing of describing in the french patent application with the No.FR0804617 submission.
Between its operating period, supplements of the present invention can be added into concentrated feed of animal (feed concentrate).This concentrated feed is well known to those skilled in the art, and can comprise soybean skin (soybean hull), grain (corn grains), oil cake, from accessory substance of the alcohol production of wheat or corn etc.
For ox, goat (caprine) or sheep (ovine) feed, can supplements of the present invention be added into roughage such as herbage, clover, hay etc.
Supplements of the present invention also can be used for preparing the animal feed that comprises concentrated feed of animal, roughage and the additive discussed.
Supplements of the present invention can according to dosage be allotted, thereby animal is taken in this supplements of 100mg to 1000mg every day.Preferably, this supplements is allotted by the dosage of 500mg/ day/animal.
Supplements of the present invention is particularly suitable for ruminant, especially ox.
Embodiment
A) in vitro test
Rumen fluid (Ruminal fluid) is collected from ox.
The solution for preparing 1: 1 (by volume) of rumen fluid and buffer solution subsequently.
The said solution of 50ml is imported PA tube.
The ration (ration) that (based on dry) preparation is made up of iblet (11.4%), soybean oil cake (1.6%), cottonseed (7.9%), maize gluten (11.6%), molasses (3.2%), fatty acid calcium salt (1.3%) and the minerals and vitamins pre-composition (2.1%) of alfalfa hay (34.7%), rye grass hay (14.8%), the barley corn (11.4%) that grinds, grinding.
Preparation is by corn, soybean oil cake, wheat and randomly get into the concentrated feed that other raw materials of concentrated feed composition are formed usually.
300mg ration, 200mg concentrated feed (it is equal to 60/40 mixture of ration and concentrated feed) and 6.25mg supplements are imported PA tube, promptly per 11 parts rumen fluid/cushioning liquid 1/1 mixture 250mg additive.
Several kinds of supplements of the present invention A1 to A4 and other supplements ET1 to ET6 that serves as contrast composition (elements for comparison) have been tested.Provide their composition and amount in the following table, explain with respect to 1 liter of rumen fluid/cushioning liquid mixture:
Additive | Eugenol (mg) | Cinnamic acid (mg) | Garlic extract (mg) |
ET1 | 125 | 125 | 0 |
|
0 | 250 | 0 |
ET3 | 250 | 0 | 0 |
|
0 | 0 | 250 |
|
0 | 125 | 125 |
ET6 | 125 | 0 | 125 |
A1 | 41.7 | 41.7 | 166.7 |
A2 | 41.7 | 166.7 | 41.7 |
A3 | 166.7 | 41.7 | 41.7 |
A4 | 83.3 | 83.3 | 83.3 |
Garlic extract used herein is by Toledo Ta Laweila-De Laleina (Talavera de la Reina; The liquid of " feed grade GARLICON 40% liquid (GARLICON 40%Liquid Feed Grade) " that the Pu Ruibiya of Spain company feed extract Toledo) (Prebia Feed Extracts S.L.) is sold, it contains garlic/onion characteristic organosulfur compound of 400ppm and 6.7% PTS and 34.3% PTSO by weight by weight.
Also test two contrasts.The one T1 is the ration that only contains roughage.
The 2nd T2 is an aqueous feed solution, gives the coban (monensin) (reactive compound that streptomycete (Streptomyces cinnamonensis) mycelium produces from Chinese cassia tree ground) of every liter of rumen fluid of this solution/cushioning liquid mixture 500mg concentration.
The pipe that seals is maintained 39 ℃ continue 24 hours to hatch.
Collect the gaseous sample of liquid top.
Measure the concentration of volatile fatty acid (VFA: acetic acid esters, malonate, butyrate, valerate) and methane.
Implement twice in each test and repeating above 2 continuous times.
Provide the result of gasmetry in the following table.
The ratio that is called C2/C3 is the ratio between acetic acid esters (C2) and the malonate (C3).
Supplements | Methane mL | Total VFAml (*) | The % malonate | The % acetic acid esters | The % butyrate | C2/C3 |
ET1 | 24.9206 | 66.33 | 54.59 | 76.84 | 6.6 | 5.58 |
ET2 | 19.4051 | 66.51 | 54.68 | 76.94 | 6.53 | 5.6 |
ET3 | 18.3533 | 64.94 | 50.26 | 76.55 | 6.64 | 5.55 |
ET4 | 23.2851 | 61.9 | 50.78 | 75.59 | 7.01 | 5.21 |
ET5 | 23.1945 | 63.44 | 51.02 | 75.97 | 6.99 | 5.37 |
ET6 | 23.3753 | 64.65 | 51.95 | 76.21 | 6.79 | 5.41 |
A1 | 18.4931 | 63.36 | 49.47 | 75.83 | 6.97 | 5.33 |
A2 | 18.4578 | 67.16 | 51.98 | 76.44 | 6.8 | 5.5 |
A3 | 21.1599 | 69.47 | 50.65 | 76.28 | 6.75 | 5.47 |
A4 | 17.9579 | 67.15 | 50.29 | 76.02 | 6.93 | 5.41 |
(*): except that malonate, acetic acid esters and butyrate, other compounds such as valerate, isovalerate or isobutyrate constitute remainder until 100%.
Can define the result's that implements statistical analysis with SAS software and to make 3 kinds of following regression equations that concentration of component is related in methane concentration Y (unit/ml) and the supplements:
Y=0.078E+0.0077C+0.096G+0.000356×E×C-0.000012×E×C×G
Wherein:
The concentration of E=eugenol (%)
The concentration of C=cinnamic acid (%)
The concentration (%) of G=garlic extract
Can be in Fig. 1 the generation of visualize methane.
Fig. 2 can the visualize volatile fatty acid generation as the function of the concentration of 3 kinds of component E, C and G in the supplements.
Observe, when the content of the extract G of green onion section increased in supplements, the generation of volatile fatty acid was lower.
The more detailed measurement extraly shows
The molar ratio of-acetic acid esters reduces under the influence of C and G combination and E, C and G combination;
The molar ratio of-butyrate increases under the influence of C and G combination and E, C and G combination;
The molar ratio of-valerate reduces under C and G combination, and under the influence of C and G combination and E, C and G combination, increases; With
The molar ratio of-malonate increases under independent C, E and G influence, and reduces with the combination of C and G.
Observe optimum range (optimal zone), also promptly, cause the maximum scope that methane produces and the best maintained volatile fatty acid produces that reduces, corresponding to the following ratio of component E, C and G in the supplements:
-30 to 45% eugenol,
-45 to 30% cinnamic acid,
-Yue 25% garlic extract.
In fact, compare with the similar foodstuff additive that does not contain the garlic extract, the supplements of the present invention with this composition causes the methane in the ruminant to produce minimizing 15 to 25%.
Thereby aforementioned result explains that clearly component eugenol, cinnamic acid and green onion section extract play a role synergistically and reduce the generation of methane significantly, especially in ruminant such as ox.
Therefore, to earth atmosphere and therefore, help to limit global warming, can take following action in order to reduce discharge of methane:
-prepare supplements of the present invention;
-this supplements is added into the feed allocation quantity (feed ration) of ruminant; And
-feed allocation quantity that obtains is fed to ruminant.
B) in vivo studies
Implement test in vivo to confirm the previous result who obtains in vivo.
I) animal of test
They have been Si Tanniu (Holstein cow).
Ii) food
Their day ration (daily ration) has the following composition that provides in the following table:
Composition | % in mass |
Maize straw (corn fodder) | 25 |
Alfalfa hay | 29 |
The alfalfa hay of chopping | 11 |
The concentrated granular material | 35 |
Amount to | 100 |
Following supplements is added into the day ration of ox:
ET7 mixture of the present invention is by forming in the garlic extract (seeing external embodiment) that is called " feed grade GARLICON 40% liquid " as the cinnamic acid and 24% (weight) of the eugenol, 43% (weight) of mass percent weight 33% (weight).
Iii) equipment and experimental technique
Every ox is furnished with the tubule that between the nostril, is connected with sleeve (muffle).
Analyze the gas of collecting continuously by said pipe through 6 time periods (25 days each time periods).
Abreast, regularly collect the percentage of rumen fluid sample with the acetic acid esters, malonate and the butyrate that exist in the middle of the total concentration of measuring volatile fatty acid and these volatile fatty acids.
Iv) result
1) generation of methane
Fig. 3 is that the unit of display is the figure of average every ox methane every day generation of gram.
Observe, the content of mixture of the present invention is high more in day ration, and methane produces and reduces greatly more.
Cooperative effect appears in test 6 demonstrations, that is to say, occurs cooperative effect when mixture E T7 of the present invention and the combination of coban solution.
2) total content of volatile fatty acid
Fig. 4 is that the unit of explanation rumen fluid is the figure of the volatile fat acid concentration of mM/L.
Observe, the value that test 3 obtains is approaching with test 1, and this meaning reduces (with reference to figure 3) although obtain tangible methane generation, and the volatile fat acid content is equal with test 1 (contrast).
In test 4, the content of volatile fatty acid is only a little less than test 1.
3) percentage of acetic acid esters, malonate and butyrate
According to each test, in Fig. 5, Fig. 6 and Fig. 7, provide the percentage of acetic acid esters, malonate and butyrate respectively.
Lack the lumen fermentation parametric measurement or even latter's situation about improving under: the content of observing acetic acid esters reduces or constant content with malonate and butyrate is constant or increase.
V) conclusion
The experiment confirm of implementing in the body absorbs the minimizing that additive of the present invention causes that methane produces, and does not influence the generation of volatile fatty acid simultaneously substantially.
Claims (19)
1. supplements comprises:
-30 to 45% eugenol,
-30 to 45% cinnamic acid, and
The extract of-10 to 40% the plant that belongs to green onion section (Alliaceae),
-randomly comprise other compounds, especially organosulfur compound remainder until 100%.
2. according to the supplements described in the claim 1, wherein, said plant belongs to allium (Allium).
3. according to the supplements described in the claim 2, wherein, said plant is a cultivation garlic (garlic (Allium sativum L.)).
4. according to the supplements described in the claim 3, it comprises 15 to 35% garlic extract.
5. according to the supplements described in the claim 4, it comprises 24 to 26% garlic extract.
6. according to each described supplements in the claim 1 to 5, wherein, said plant extracts comprise at least a with the corresponding compound of following formula F:
R-SO
a-S-R′
Wherein:
R and R ' are identical or different, represent alkyl separately; And a is 1 or 2.
7. supplements, it comprises:
-30 to 45% eugenol,
-30 to 45% cinnamic acid, and
-10 to 40% with the corresponding at least a compound of following formula F:
R-SO
a-S-R′
Wherein:
R and R ' are identical or different, represent alkyl separately; And a is 1 or 2;
-randomly comprise other compounds, especially organosulfur compound remainder until 100%.
8. according to the supplements described in claim 6 or 7, wherein, in the F formula, radicals R and R ' are the alkyl that comprises 1 to 5 carbon atom.
9. the supplements described in according to Claim 8, wherein, in the F formula, said radicals R and R ' are propyl group.
10. according to each described supplements in the claim 6 to 9, wherein, said R is identical with R ' base.
11. according to each described supplements in the claim 6 to 10, wherein, in the F formula, a is 1.
12. according to the supplements described in claim 6 or 7, wherein, the compound of said formula F is a sulfo-sulfinic acid dipropyl.
13. according to each described supplements in the claim 6 to 10, wherein, in the F formula, a is 2.
14. according to the supplements described in claim 6 or 7, wherein, the compound of said formula F is the thiosulfonic acid dipropyl.
15. according to the supplements described in claim 6 or 7, it comprises the mixture of sulfo-sulfinic acid dipropyl and thiosulfonic acid dipropyl.
16. the purposes of each described supplements in the claim 1 to 15 is used for reducing the generation of ruminant methane.
17. the purposes of each described supplements and coban combination is used for reducing the generation of ruminant methane in the claim 1 to 15.
18. according to the purposes described in claim 16 or 17, said ruminant is an ox.
19. a method that is used for reducing discharge of methane to earth atmosphere, it may further comprise the steps:
-each described supplements in the claim 1 to 15 is added into the feed allocation quantity of ruminant; And
-feed allocation quantity that obtains is fed to ruminant.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH1086/09 | 2009-07-13 | ||
CH01086/09A CH701425A1 (en) | 2009-07-13 | 2009-07-13 | Food additive, useful for reducing methane production in a ruminant, which is a cow, comprises eugenol and cinnamaldehyde, which additive further comprises at least one plant extract belonging to the Alliaceae families |
FR0903439A FR2947698B1 (en) | 2009-07-13 | 2009-07-13 | FOOD ADDITIVE CONTAINING AT LEAST ONE DIALKYL THIOSULFINATE OR THIOSULFONATE |
FR0903439 | 2009-07-13 | ||
PCT/CH2010/000177 WO2011006269A1 (en) | 2009-07-13 | 2010-07-08 | Food additive containing eugenol, cinnamaldehyde and an alliaceous extract |
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CN102573512A true CN102573512A (en) | 2012-07-11 |
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CN2010800313218A Pending CN102573512A (en) | 2009-07-13 | 2010-07-08 | Food additive containing eugenol, cinnamaldehyde and an alliaceous extract |
Country Status (7)
Country | Link |
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US (1) | US20120171323A1 (en) |
EP (1) | EP2453760B1 (en) |
CN (1) | CN102573512A (en) |
CA (1) | CA2771903A1 (en) |
ES (1) | ES2440086T3 (en) |
RU (1) | RU2012102453A (en) |
WO (1) | WO2011006269A1 (en) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2014013068A1 (en) * | 2012-07-20 | 2014-01-23 | Dr. Eckel Gmbh | Combination for reducing methane emissions in ruminant feeding |
US20150238579A1 (en) * | 2014-02-26 | 2015-08-27 | Investfood, LLC | Methods for assembling a product for modulating an immunomodulatory response in humans |
KR101656929B1 (en) * | 2014-06-02 | 2016-09-13 | 씨제이제일제당 (주) | Feed additive composition for reducing methane gas produced by ruminant animals |
US20200170281A1 (en) * | 2017-06-01 | 2020-06-04 | Mootral Sa | Animal feed supplement |
US20220256890A1 (en) * | 2019-07-10 | 2022-08-18 | Mootral Innovations Limited | Animal feed supplement and animal feed composition |
CN110495593A (en) * | 2019-08-27 | 2019-11-26 | 麻江县明洋食品有限公司 | A kind of open country green onion red acid soup preparation method |
AU2021396978A1 (en) | 2020-12-08 | 2023-02-23 | Ruminant Biotech Corp Limited | Improvements to devices and methods for delivery of substances to animals |
Citations (3)
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WO2003094628A1 (en) * | 2002-05-14 | 2003-11-20 | Akzo Nobel Nv | Method for reducing methane production emanating from the digestive activities of an animal |
CN1672738A (en) * | 2005-02-05 | 2005-09-28 | 浙江大学 | Deodorizing additive of compound plant extract for farm animal and its use |
WO2008037827A1 (en) * | 2006-09-27 | 2008-04-03 | Dmc Research Center, S.L. | Use of particular antibacterial compounds, which are derived from alliaceae, as natural additives in animal feed |
Family Cites Families (5)
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DK137622B (en) * | 1973-05-15 | 1978-04-10 | Ivar Harry Sanick | Method and means of protection of food or stimulants against attack by microorganisms. |
FR2813884B1 (en) | 2000-09-12 | 2004-01-16 | Critt Innophyt | PROCESS FOR PRODUCING THIOSULFINATES AND DEVICE FOR CARRYING OUT THIS PROCESS |
FR2853550B1 (en) * | 2003-04-14 | 2007-08-17 | Phytosynthese | FOOD SUPPLEMENT FOR ANIMALS, ESPECIALLY POULTRY |
EP1721534A1 (en) | 2004-03-03 | 2006-11-15 | Mousala, S. L. | Use of extracts and compounds of allium-genus plants as preservatives in the food and agri-food industries |
FR2881321B1 (en) * | 2005-02-03 | 2008-12-26 | Axiss France Sas Soc Par Actio | FOOD ADDITIVE FOR RUMINANTS BASED ON EUGENOL AND CINNAMALDEHYDE |
-
2010
- 2010-07-08 US US13/383,194 patent/US20120171323A1/en not_active Abandoned
- 2010-07-08 CA CA2771903A patent/CA2771903A1/en not_active Abandoned
- 2010-07-08 WO PCT/CH2010/000177 patent/WO2011006269A1/en active Application Filing
- 2010-07-08 RU RU2012102453/13A patent/RU2012102453A/en unknown
- 2010-07-08 ES ES10742700.7T patent/ES2440086T3/en active Active
- 2010-07-08 EP EP10742700.7A patent/EP2453760B1/en not_active Not-in-force
- 2010-07-08 CN CN2010800313218A patent/CN102573512A/en active Pending
Patent Citations (3)
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WO2003094628A1 (en) * | 2002-05-14 | 2003-11-20 | Akzo Nobel Nv | Method for reducing methane production emanating from the digestive activities of an animal |
CN1672738A (en) * | 2005-02-05 | 2005-09-28 | 浙江大学 | Deodorizing additive of compound plant extract for farm animal and its use |
WO2008037827A1 (en) * | 2006-09-27 | 2008-04-03 | Dmc Research Center, S.L. | Use of particular antibacterial compounds, which are derived from alliaceae, as natural additives in animal feed |
Non-Patent Citations (1)
Title |
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MACHEBOEUF, ET AL: "Dose-response effects of essential oils on in vitro fermentation activity of the rumen microbial population", 《ANIMAL FEED SCIENCE AND TECHNOLOGY》 * |
Also Published As
Publication number | Publication date |
---|---|
EP2453760A1 (en) | 2012-05-23 |
US20120171323A1 (en) | 2012-07-05 |
RU2012102453A (en) | 2013-08-20 |
ES2440086T3 (en) | 2014-01-27 |
CA2771903A1 (en) | 2011-01-20 |
EP2453760B1 (en) | 2013-09-18 |
WO2011006269A1 (en) | 2011-01-20 |
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