CN102533902A - Method for preparing sodium hyaluronate from a non-animal protein source - Google Patents
Method for preparing sodium hyaluronate from a non-animal protein source Download PDFInfo
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- CN102533902A CN102533902A CN201010620329XA CN201010620329A CN102533902A CN 102533902 A CN102533902 A CN 102533902A CN 201010620329X A CN201010620329X A CN 201010620329XA CN 201010620329 A CN201010620329 A CN 201010620329A CN 102533902 A CN102533902 A CN 102533902A
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- animal protein
- protein source
- ethanol
- sodium hyaluronate
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Abstract
The invention discloses a method for preparing sodium hyaluronate from a non-animal protein source. According to the method, soy protein, a yeast extract and corn protein used as nitrogen sources for fermentation are used for producing the sodium hyaluronate instead of an animal nitrogen source, and the moisture retention of sodium hyaluronate prepared by adopting the method disclosed by the invention is enhanced by 20 percent than that of hyaluronic acid prepared from an animal protein source, the cost can be saved by 40-45 percent, and the quality of the non-animal protein source sodium hyaluronate is improved.
Description
Affiliated technical field
The present invention relates to non-animal protein source hyaluronate sodium preparation method, belong to technical field of bioengineering.
Background technology:
Protein source in China's fermentative prodn hyaluronate sodium process is used animal proteinum peptone, yeast extract paste or compound nitrogen sources such as leach liquor, beef extract usually at present, and cost is very high, and strives foodstuff raw material with other animals in addition.The present invention just provides a kind of non-animal protein source hyaluronate sodium preparation method, promptly utilizes non-animal protein source as fermentation nitrogen source, successfully prepares the hyaluronate sodium that has good quality; Its mucinase water absorbing properties is more outstanding; The performance of shrinking is outstanding, can win more human consumers' favor with the moisture retention of the mucinase high 20% produced than animal protein source; Simultaneously can save cost 40-45%, and improve the quality of product.Embodiment of the present invention can thoroughly solve because of the protein source problem in fermentative prodn hyaluronate sodium process.
Summary of the invention
The present invention provides a kind of non-animal protein source hyaluronate sodium preparation method; It is characterized in that the streptococcic fermentating formula of choosing through wild-type is that non-animal protein source comprises that Sunlover 10, yeast extract paste, zein are as the nitrogenous source that ferments; Add that Sodium Glutamate, amino acid, glucose, potassium primary phosphate, magnesium chloride, UTP form; Fermented liquid through fermentation obtains is squeezed into throw out in the retort with toothed gear pump through filtering post precipitation; Through dewatering of ethanol, simultaneously wherein protein, colloid, nucleic acid, impurity are removed, the hyaluronate sodium water cut is dropped to less than about 4%.
The present invention can replace animal nitrogenous source production hyaluronate sodium as the nitrogenous source of fermentation with Sunlover 10, yeast extract paste, zein; Its quality is than the moisture retention of the mucinase high 20% of animal protein source production; Simultaneously can save cost 40-45%, and improve the quality of product.
Technical scheme:
A kind of non-animal protein source hyaluronate sodium preparation method; It is characterized in that the streptococcic fermentating formula of choosing through wild-type is that non-animal protein source comprises that Sunlover 10, yeast extract paste, zein are as the nitrogenous source that ferments; Add that Sodium Glutamate, amino acid, glucose, potassium primary phosphate, magnesium chloride, UTP form, the PH of fermenting process is controlled at 6.4-8.4, and rotating speed is 550r/min; Temperature is controlled at 37 ℃; System fermented liquid throw out is squeezed in the retort with toothed gear pump through filtering post precipitation, add dewatering of ethanol through secondary, simultaneously wherein protein, colloid, nucleic acid, impurity are dissolved in the ethanol; Discharge ethanol, remove wherein protein, colloid, nucleic acid, impurity simultaneously; Again with vacuum pump with being evacuated in the jar, the hyaluronate sodium water cut is dropped to less than 4%.
The invention has the advantages that:
The present invention can replace animal nitrogenous source production hyaluronate sodium as the nitrogenous source of fermentation with Sunlover 10, yeast extract paste, zein; Its quality is than the moisture retention of the mucinase high 20% of animal protein source production; Simultaneously can save cost 40-45%, and improve the quality of product.
Embodiment:
Enforcement of the present invention is like this: the streptococcic fermentating formula of choosing through wild-type is that non-animal protein source comprises that Sunlover 10, yeast extract paste, zein are as the nitrogenous source that ferments; Add that Sodium Glutamate, amino acid, glucose, potassium primary phosphate, magnesium chloride, UTP form; The PH of fermenting process is controlled at 6.4-8.4, and rotating speed is 550r/min, and temperature is controlled at 37 ℃; System fermented liquid throw out is squeezed in the retort with toothed gear pump through filtering post precipitation; Inject 95% ethanol suitable through the ethanol opening for feed, carry out dewatering in 1 hour, wherein protein, colloid, nucleic acid, impurity is dissolved in the ethanol with reaction mass; Waste ethanol is discharged through the ethanol discharge port, removes wherein protein, colloid, nucleic acid, impurity and water simultaneously; For the second time inject 95% fresh ethanol, dewatered again 1 hour; Discharge ethanol, and close all valves; With being evacuated in the jar, temperature keeps 50 ℃ in jar with vacuum pump; So dry 20 hours, the hyaluronate sodium water cut is dropped to less than 4%, from discharge hole for discharge, packing after detecting.
Claims (1)
1. non-animal protein source hyaluronate sodium preparation method; It is characterized in that the streptococcic fermentating formula of choosing through wild-type is that non-animal protein source comprises that Sunlover 10, yeast extract paste, zein are as the nitrogenous source that ferments; Add that Sodium Glutamate, amino acid, glucose, potassium primary phosphate, magnesium chloride, UTP form, the PH of fermenting process is controlled at 6.4-8.4, and rotating speed is 550r/min; Temperature is controlled at 37 ℃; The fermented liquid that makes is squeezed into throw out in the retort with toothed gear pump through filtering post precipitation, adds dewatering of ethanol through secondary, simultaneously wherein protein, colloid, nucleic acid, impurity is dissolved in the ethanol; Discharge ethanol, remove wherein protein, colloid, nucleic acid, impurity simultaneously; Again with vacuum pump with being evacuated in the jar, the hyaluronate sodium water cut is dropped to less than 4%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010620329XA CN102533902A (en) | 2010-12-22 | 2010-12-22 | Method for preparing sodium hyaluronate from a non-animal protein source |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010620329XA CN102533902A (en) | 2010-12-22 | 2010-12-22 | Method for preparing sodium hyaluronate from a non-animal protein source |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102533902A true CN102533902A (en) | 2012-07-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010620329XA Pending CN102533902A (en) | 2010-12-22 | 2010-12-22 | Method for preparing sodium hyaluronate from a non-animal protein source |
Country Status (1)
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| CN (1) | CN102533902A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1984003302A1 (en) * | 1983-02-18 | 1984-08-30 | Diagnostic Inc | Hyaluronic acid from bacterial culture |
| CN1587386A (en) * | 2004-07-26 | 2005-03-02 | 清华大学 | Method for producing hyaluronic acid and its special strain |
| CN101041843A (en) * | 2006-03-24 | 2007-09-26 | 张伟 | Method for producing hyaluronic acid by using microbiofermentation technique |
-
2010
- 2010-12-22 CN CN201010620329XA patent/CN102533902A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1984003302A1 (en) * | 1983-02-18 | 1984-08-30 | Diagnostic Inc | Hyaluronic acid from bacterial culture |
| CN1587386A (en) * | 2004-07-26 | 2005-03-02 | 清华大学 | Method for producing hyaluronic acid and its special strain |
| CN101041843A (en) * | 2006-03-24 | 2007-09-26 | 张伟 | Method for producing hyaluronic acid by using microbiofermentation technique |
Non-Patent Citations (2)
| Title |
|---|
| 《无锡轻工大学学报》 19990930 高海军 等 "兽疫链球菌摇瓶发酵法生产透明质酸" 第18卷, 第3期 * |
| 高海军 等: ""兽疫链球菌摇瓶发酵法生产透明质酸"", 《无锡轻工大学学报》 * |
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Application publication date: 20120704 |