CN102530802B - reagent filling device and method - Google Patents

reagent filling device and method Download PDF

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Publication number
CN102530802B
CN102530802B CN201010619617.3A CN201010619617A CN102530802B CN 102530802 B CN102530802 B CN 102530802B CN 201010619617 A CN201010619617 A CN 201010619617A CN 102530802 B CN102530802 B CN 102530802B
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storage tank
liquid storage
reagent
valve
pressure
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CN102530802A (en
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申涛
滕锦
郭文恒
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Abstract

The invention discloses a kind of reagent filling device, comprise at least one first liquid storage tank and at least one second liquid storage tank, described at least one first liquid storage tank connects the first outside positive pressure sources and negative source by valve, described at least one second liquid storage tank connects the second outside positive pressure sources, the pressure that described first positive pressure sources provides is greater than the pressure that described second positive pressure sources provides, described at least one first liquid storage tank is connected by valve with at least one second liquid storage tank, described at least one first liquid storage tank is also connected to reagent container by valve, described reagent container carries out reagent perfusion at least one first liquid storage tank, described at least one first liquid storage tank pours at least one second liquid storage tank.The invention also discloses a kind of reagent method for filling.Reagent filling device of the present invention and method have less liquid storage tank afunction time and less gas flow consumption.

Description

Reagent filling device and method
Technical field
The present invention relates to reagent perfusion field, particularly a kind of reagent filling device and method being applicable to fluid analyte meter.
Background technology
In analytic instrument, often use different pressure to drive same reagent flows, need reagent to be stored in liquid storage tanks different in instrument, then use different pressure to drive, namely need to carry out reagent perfusion.Referring to Fig. 1, is the part fluid path structural representation of prior art reagent perfusion.First liquid storage tank 2 connects positive pressure sources S1 and negative source S3, and the second liquid storage tank 3 connects positive pressure sources S2 and negative source S3.Positive pressure sources S1, S2 and negative source S3 provide positive pressure P1 respectively, P2 and negative pressure P3.The driving reagent flows of the first liquid storage tank 2 be pressure P 1, use negative pressure P3 during perfusion reagent.The pressure of the driving reagent flows of the second liquid storage tank 3 is P2, uses negative pressure P3 during perfusion reagent.Negative pressure P3 is communicated with liquid storage tank 2 by pressure-gradient control valve 1 with malleation P1.Negative pressure P3 is communicated with liquid storage tank 3 by pressure-gradient control valve 6 with malleation P2.Reagent bottle 9 is communicated with liquid storage tank 2 by fluid path control cock 7, and reagent bottle 9 is also communicated with the second liquid storage tank 3 by fluid path control cock 8 simultaneously.There is bottom liquid storage tank 2 reagent interface 4.There is bottom liquid storage tank 3 reagent interface 5.In use, pressure-gradient control valve 1,6 and fluid path control cock 7,8 are all closed, and the liquid in liquid storage tank 2 flows out under pressure P 1 acts on from the reagent interface 4 of liquid storage tank 2.Liquid in liquid storage tank 3 flows out under the left and right of pressure P 2 from reagent interface 5.
Liquid storage tank 2 is when pouring into, and first open pressure-gradient control valve 1, then open fluid path control cock 7, the reagent now in reagent bottle 9 enters liquid storage tank 2 at the flows by action of negative pressure P3.First open pressure-gradient control valve 6, then open fluid path control cock 8, the reagent in reagent bottle 9 enters liquid storage tank 3 at negative pressure P3 flows by action.
As mentioned above, liquid storage tank 2 and liquid storage tank 3 are when carrying out reagent perfusion, and its inside is negative pressure P3, and reagent cannot be driven to flow in other accepting containers, causes liquid storage tank 2 and liquid storage tank 3 afunction.In addition, liquid storage tank 2 and liquid storage tank 3 independently carry out reagent perfusion, and need to carry out pressure switching separately, namely need the malleation P1 in liquid storage tank 2 and liquid storage tank 3, P2 switches to negative pressure P3, reagent could be drawn in corresponding liquid storage tank like this.And malleation is switched to switch to malleation from negative pressure after negative pressure and perfusion complete and all need the regular hour, also need to consume a large amount of airsheds.Therefore the perfusion operating time of reagent of the prior art is long, and the afunction time is long, and gas flow consumption is large.
Summary of the invention
Causing the afunction time long and the problem that gas flow consumption is large to solve the perfusion of prior art reagent, being necessary to provide a kind of reagent filling device that can overcome the problems referred to above.
There is a need to provide a kind of reagent method for filling.
A kind of reagent filling device, comprise at least one first liquid storage tank and at least one second liquid storage tank, described at least one first liquid storage tank connects the first outside positive pressure sources and negative source by valve, described at least one second liquid storage tank connects the second outside positive pressure sources, the pressure that described first positive pressure sources provides is greater than the pressure that described second positive pressure sources provides, described at least one first liquid storage tank is connected by valve with at least one second liquid storage tank, described at least one first liquid storage tank is also connected to reagent container by valve, described reagent container carries out reagent perfusion at least one first liquid storage tank, described at least one first liquid storage tank pours at least one second liquid storage tank.
In reagent filling device of the present invention, described reagent filling device comprises first liquid storage tank and second liquid storage tank, and described first liquid storage tank connects described first positive pressure sources and negative source by valve, and described second liquid storage tank connects described second pressure source.
In reagent filling device of the present invention, described reagent filling device comprises multiple first liquid storage tank and second liquid storage tank, described multiple first liquid storage tank connects described first positive pressure sources and negative source by valve, and described second liquid storage tank connects described second pressure source.
In reagent filling device of the present invention, described reagent filling device comprises first liquid storage tank and multiple second liquid storage tank, described first liquid storage tank connects described first positive pressure sources and negative source by valve, and described multiple second liquid storage tank connects described second pressure source.
In reagent filling device of the present invention, described reagent filling device comprises multiple first liquid storage tank and multiple second liquid storage tank, described multiple first liquid storage tank connects described first positive pressure sources and negative source by valve, and described multiple second liquid storage tank connects described second pressure source.
A kind of reagent method for filling, comprise the first liquid storage tank and the second liquid storage tank that provide and connected by valve, in the first liquid storage tank, pressure is greater than pressure in the second liquid storage tank; There is provided the reagent container that reagent is housed, reagent container is connected to the first liquid storage tank by valve; Reagent container carries out reagent perfusion to the first liquid storage tank; First liquid storage tank carries out reagent perfusion to the second liquid storage tank.
In reagent method for filling of the present invention, described reagent container carries out reagent perfusion to the first liquid storage tank and comprises the valve opened between the first liquid storage tank and negative source, makes the first liquid storage tank inside switch to negative pressure; Open the valve between the first liquid storage tank and reagent container, in reagent container, reagent is filled in the first liquid storage tank under vacuum suction.
In reagent method for filling of the present invention, described first liquid storage tank carries out reagent to the second liquid storage tank and pours into the valve comprised between cut out first liquid storage tank and reagent container, and the reagent perfusion of the first liquid storage tank stops; Close the valve between the first liquid storage tank and negative source, the first liquid storage tank internal pressure switches to malleation to open valve between the first liquid storage tank and the second liquid storage tank, and in the first liquid storage tank, reagent is filled in the second liquid storage tank.
A kind of reagent method for filling, comprise and multiple first liquid storage tank and multiple second liquid storage tank are provided, in first liquid storage tank, pressure is greater than pressure in connected second liquid storage tank, and the first liquid storage tank is connected with negative source by valve, is connected between the first liquid storage tank and the second liquid storage tank by valve; There is provided reagent container, reagent container is connected by valve with the first liquid storage tank; Reagent container carries out reagent perfusion to the first liquid storage tank; First liquid storage tank carries out reagent perfusion to the second liquid storage tank.
In reagent method for filling of the present invention, reagent container carries out reagent to the first liquid storage tank and pours into the valve comprised between closedown first liquid storage tank and the second liquid storage tank; Open the valve between negative source and the first liquid storage tank, make the first liquid storage tank inside be negative pressure; Open the valve between the first liquid storage tank and reagent container, the reagent in reagent container is filled in the first liquid storage tank.
In reagent method for filling of the present invention, the first liquid storage tank carries out reagent to the second liquid storage tank and pours into the valve comprised between cut out first liquid storage tank and reagent container; Close the valve between the first liquid storage tank and negative source; Open the valve between the first liquid storage tank and the second liquid storage tank, the reagent in the first liquid storage tank is filled in the second corresponding liquid storage tank.
In reagent method for filling of the present invention, described reagent method for filling also comprises: close the valve between the first liquid storage tank and the second liquid storage tank, open the valve between the first liquid storage tank and negative source, open the valve between the first liquid storage tank and reagent container, reagent perfusion is carried out to the first liquid storage tank.
Compared to prior art, reagent filling device of the present invention adopts reagent container to pour into the first liquid storage tank and mode that the first liquid storage tank pours into the second liquid storage tank, the liquid storage tank afunction time can be reduced, and reduce airshed consumption, there is less afunction time and less gas flow consumption, can also be cost-saving.Reagent method for filling of the present invention adopts reagent container to pour into the first liquid storage tank and mode that the first liquid storage tank pours into the second liquid storage tank, the liquid storage tank afunction time can be reduced, and reduce airshed consumption, there is less afunction time and less gas flow consumption.
In addition, reagent filling device of the present invention and method are for multiple first liquid storage tank and second liquid storage tank, first liquid storage tank and multiple second liquid storage tank, and the situation of multiple first liquid storage tank and multiple second liquid storage tank combination, in the first liquid storage tank, malleation is greater than on the basis of malleation in the second liquid storage tank, by reagent container, reagent perfusion is carried out to the first liquid storage tank, by the first liquid storage tank, reagent perfusion is being carried out to corresponding second liquid storage tank, ensure that the second liquid storage tank still has normal function in filling process, and there is less gas flow consumption, save cost.
Accompanying drawing explanation
Fig. 1 is the part fluid path structural representation of prior art reagent perfusion;
Fig. 2 is the part fluid path structural representation of reagent filling device one better embodiment of the present invention;
Fig. 3 is the schematic flow sheet of a reagent method for filling of the present invention embodiment;
Fig. 4 is the schematic flow sheet of another embodiment of reagent method for filling of the present invention.
Detailed description of the invention
By reference to the accompanying drawings the present invention is described in further detail below by detailed description of the invention.
Referring to Fig. 2, is the part fluid path structural representation of reagent filling device one better embodiment of the present invention.In Fig. 1, unlabelled fine rule represents sebific duct, and the connection related in the following describes all is represented and to be connected by sebific duct.Described reagent filling device comprise the first liquid storage tank 11, second liquid storage tank 10, with the first reagent interface 17 be connected bottom the first liquid storage tank 11, with the second reagent interface 13, the reagent bottle 16 that are connected bottom the second liquid storage tank 10.Wherein, reagent bottle 16 is connected to the first liquid storage tank 11 upper opening by the first fluid path control cock 15.The bottom of the first liquid storage tank 11 is connected to the second liquid storage tank 10 upper opening by the second fluid path control cock 12.First liquid storage tank 11 is connected to the first positive pressure sources S1 and negative source S3 by the first pressure-gradient control valve 14.Second liquid storage tank 10 connects the second positive pressure sources S2.Wherein, first, second positive pressure sources S1, S2 and negative source S3 provide malleation P1 respectively, P2 and negative pressure P3, and the size of pressure P 1 is greater than the size of pressure P 2.In the present embodiment, described pressure source S1, S2, S3 are gas pressure source.
When pouring into, close the second fluid path control cock 12, open the first pressure-gradient control valve 14 and the first fluid path control cock 15, first liquid storage tank 11 internal gas pressure is switched to P3 from P1.Under negative pressure P3 effect, reagent is filled into the first liquid storage tank 11 from reagent bottle 16.
Close the first fluid path control cock 15, first pressure-gradient control valve 14, open the second fluid path control cock 12, the first liquid storage tank 11 internal gas pressure is made to be switched to malleation P1 by negative pressure P3, pressure due to malleation P1 is greater than the pressure of P2, then in the first liquid storage tank 11, reagent is perfused in the second liquid storage tank 10 under difference of pressure (P1-P2) effect.
After considering that the first liquid storage tank 11 pours into the second liquid storage tank 10, the reagent of the first liquid storage tank 11 can reduce, as continued to pour into the first liquid storage tank 11, the second fluid path by-pass valve control 12 can be closed, open the first pressure control valve 14 and the first fluid path by-pass valve control 15, make the first liquid storage tank 11 internal gas pressure be negative pressure P3, thus proceed reagent perfusion.In fact, complete the perfusion to the first liquid storage tank 11 and the second liquid storage tank 10, be substantially equal to the amount of reagent sum stored by the first liquid storage tank 11 and the second liquid storage tank 10 from reagent bottle 16 amount of reagent be filled into the first liquid storage tank 11.
Complete the perfusion of two liquid storage tanks and described embodiment to prior art below to complete to pour into the first liquid storage tank and the second liquid storage tank reagent and contrast.
In prior art, the perfusion completing two liquid storage tanks needs to pour into respectively two liquid storage tanks, assuming that it is t1 that malleation P1 switches to switching time needed for negative pressure P3, gas average discharge is Q1, and infusion time is t2; It is t3 that negative pressure P3 switches to switching time needed for malleation P1, and gas average discharge is Q2; It is t4 that malleation P2 switches to switching time needed for negative pressure P3, and gas average discharge is Q3, and infusion time is t5; It is t6 that negative pressure P3 switches to malleation P2 required time, and gas average discharge is Q4.As can be seen here, two liquid storage tank afunction times are respectively t1+t2+t3 and t4+t5+t6, and negative pressure air consumption is Q1*t1+Q3*t4, and malleation air consumption is Q2*t3+Q4*t6.
So similarly, so that in prior art, liquid storage tank switching air pressure and infusion time are for reference, state on the invention in embodiment, it is t1 that the first liquid storage tank 11 switches to switching time needed for negative pressure P3 from malleation P1, gas average discharge is Q1, and infusion time is t2+t5; Switching to switching time needed for malleation P1 from negative pressure P3 is t3, and gas average discharge is Q2.Because in the second liquid storage tank 10, air pressure is P2 all the time, therefore the reagent perfusion of the second liquid storage tank 10 does not exist because air pressure switches the time spent.
The reagent filling device first liquid storage tank 11 afunction time of the present invention is t1+t2+t3+t5, and the second liquid storage tank 10 afunction time was 0.Negative pressure air consumption is Q1*t1, and malleation air consumption is Q2*t3.Compared to prior art, reagent filling device of the present invention decreases the time of the liquid storage tank afunction caused in reagent filling process, reduces airshed demand simultaneously.In addition, the second liquid storage tank 10 need not connect negative source S3, also saves the pressure control valve between negative source S3 and the second liquid storage tank 10.
In summary, the present invention adopts that reagent container 16 pours into the first liquid storage tank 11 and the reagent filling device of the first liquid storage tank 11 to the mode that the second liquid storage tank 10 pours into can reduce the liquid storage tank afunction time, and reduce airshed consumption, cost-saving.In addition, compared with prior art, eliminate the second liquid storage tank 10 and negative pressure source S3 straight between valve, also play cost-saving effect.
In other embodiments of reagent filling device of the present invention, the number of the first liquid storage tank can be multiple, and the second liquid storage tank also can be multiple.Such as: reagent container pours into first liquid storage tank, this first liquid storage tank pours into the second liquid storage tank that multiple internal pressure is less than this first liquid storage tank, and so multiple second liquid storage tank all keeps malleation when pouring into, and has normal function.Again such as: reagent container pours into multiple first liquid storage tank, the plurality of first liquid storage tank pours into the second liquid storage tank that an internal pressure is less than the plurality of first liquid storage tank.
In other embodiments, the combination of above-mentioned several situation can also be comprised, such as: multiple first liquid storage tank pours into multiple second liquid storage tank, wherein can comprise multiple first liquid storage tank and pour into second liquid storage tank, multiple second liquid storage tank of first liquid storage tank perfusion, and first liquid storage tank pours into the combinations organizing situations such as second liquid storage tank more, only the pressure that need meet in the first liquid storage tank is greater than the pressure of the second liquid storage tank of corresponding perfusion.
In foregoing description, the first liquid storage tank 11, second liquid storage tank 10 and pressure P 1, P2, P3 do not refer in particular to generation or limit, and are all to reach the clear object described.In fact, in reagent filling device of the present invention, the pressure size of the pressure source that the first liquid storage tank 11 connects is greater than the pressure source pressure size that the second liquid storage tank 10 connects.
Please refer to Fig. 3, be the schematic flow sheet of an embodiment of reagent method for filling of the present invention, for convenience of description, in present embodiment, adopt same label with the embodiment of reagent filling device shown in Fig. 2.Comprising of described reagent method for filling:
(1) step 101, is provided pressure P 1 in the first liquid storage tank 11 and the second liquid storage tank 10, first liquid storage tank 11 connected by valve to be greater than pressure P 2, first liquid storage tank 11 in the second liquid storage tank 10 and connects negative source by valve;
Wherein, second liquid storage tank 10 is connected (as the second fluid path control cock 12 in Fig. 2) by valve with the first liquid storage tank 11, described first liquid storage tank 11 and the second liquid storage tank 10 are connected respectively to different positive pressure sources (S1 as shown in Figure 2, S2), and the pressure of positive pressure sources that the first liquid storage tank 11 connects is greater than the pressure of the positive pressure sources that the second liquid storage tank connects, the first liquid storage tank 11 is also connected to negative source (as shown in Figure 2 negative source S3) by valve (as shown in Figure 2 the first pressure-gradient control valve 14).
(2) step 102, provides the reagent container 16 that reagent is housed, and reagent container 16 is connected to the first liquid storage tank 11 by valve (as shown in Figure 2 the first fluid path control cock 15);
(3) reagent container carries out reagent perfusion to the first liquid storage tank; Specifically comprise:
Step 103, closes the valve between the first liquid storage tank 11 and the second liquid storage tank 10;
Step 104, opens the valve between the first liquid storage tank 11 and negative source, makes the first liquid storage tank 11 inside switch to negative pressure;
Step 105, opens the valve between the first liquid storage tank 11 and reagent container 16, and in reagent container 16, reagent is filled in the first liquid storage tank 11 under vacuum suction;
(4) first liquid storage tanks carry out reagent perfusion to the second liquid storage tank; Specifically comprise:
Step 106, closes the valve between the first liquid storage tank 11 and reagent container 16, and the reagent perfusion of the first liquid storage tank 11 stops;
Step 107, closes the valve between the first liquid storage tank 11 and negative source, and the first liquid storage tank 11 internal pressure switches to malleation;
Step 108, open the valve between the first liquid storage tank 11 and the second liquid storage tank 10, in the first liquid storage tank 11, reagent is filled in the second liquid storage tank 10; Because the first liquid storage tank 11 internal pressure is greater than the second liquid storage tank internal pressure, so reagent is filled in the second liquid storage tank under difference of pressure effect in the first liquid storage tank.
In the above-described embodiment, positive pressure sources and negative source are all pneumatic supplies.When the first liquid storage tank 11 pours into the second liquid storage tank 10, the first liquid storage tank 11 and the second liquid storage tank 10 inside are all positive pressures, so its interior reagent can flow into other containers, have normal liquid storage tank function.In addition, the second liquid storage tank 10 without the need to switching its internal pressure when carrying out reagent perfusion, saves the time that pressure switches, saves airshed simultaneously, reduce the requirement to pressure source.
First liquid storage tank and second liquid storage tank are provided in above-mentioned embodiment, in fact, in examination, other change in embodiment, multiple first liquid storage tank and second liquid storage tank can also be provided, or first liquid storage tank and multiple second liquid storage tank, or multiple first liquid storage tank and multiple second liquid storage tank.In the first liquid storage tank, positive pressure is greater than on the basis of the second liquid storage tank, carries out reagent perfusion by reagent container to the first liquid storage tank, is carrying out reagent perfusion by the first liquid storage tank to the second liquid storage tank.
Referring to Fig. 4, is the schematic flow sheet of another embodiment of reagent method for filling of the present invention.This reagent method for filling comprises:
(1) step 111, multiple first liquid storage tank and multiple second liquid storage tank are provided, in first liquid storage tank, pressure is greater than pressure in connected second liquid storage tank, and the first liquid storage tank is connected with negative source by valve, is connected between the first liquid storage tank and the second liquid storage tank by valve; Wherein, first liquid storage tank and the pressure source being connected different size pressure in the second liquid storage tank respectively, and pressure is greater than pressure in connected second liquid storage tank in the first liquid storage tank, the first liquid storage tank is connected to negative source by valve, and multiple first liquid storage tank connects the second corresponding liquid storage tank;
(2) step 112, provides reagent container, and reagent container is connected by valve with the first liquid storage tank;
(3) reagent container carries out reagent perfusion to the first liquid storage tank; Specifically comprise:
Step 113, closes the valve between the first liquid storage tank and the second liquid storage tank;
Step 114, opens the valve between negative source and the first liquid storage tank, makes the first liquid storage tank inside be negative pressure;
Step 115, opens the valve between the first liquid storage tank and reagent container, and the reagent in reagent container is filled in the first liquid storage tank;
(4) first liquid storage tanks carry out reagent perfusion to the second liquid storage tank; Specifically comprise:
Step 116, closes the valve between the first liquid storage tank and reagent container;
Step 117, closes the valve between the first liquid storage tank and negative source;
Step 118, opens the valve between the first liquid storage tank and the second liquid storage tank, and the reagent in the first liquid storage tank is filled in the second corresponding liquid storage tank;
Step 119, closes the valve between the first liquid storage tank and the second liquid storage tank, opens the valve between the first liquid storage tank and negative source, opens the valve between the first liquid storage tank and reagent container, carries out reagent perfusion to the first liquid storage tank.
In the above-described embodiment, reagent container first carries out reagent perfusion to the first liquid storage tank that inside is negative pressure, and then the first liquid storage tank utilizes difference of pressure to pour into the second liquid storage tank.First liquid storage tank is carried out to the first liquid storage tank afunction in the process of reagent perfusion but the second liquid storage tank still keeps normal function.And the second liquid storage tank is being carried out in the process of reagent perfusion, first liquid storage tank and the second liquid storage tank all have normal function, therefore, compared to prior art, This embodiment reduces the afunction time of the first liquid storage tank and the second liquid storage tank in filling process, decrease the blocked operation of positive pressure sources and negative source simultaneously, decrease gas flow consumption, reduce the requirement to pressure source, cost-saving.
It should be noted that to address on reagent method for filling of the present invention and change in embodiment, first liquid storage tank can connect multiple second liquid storage tank, and first liquid storage tank can carry out reagent perfusion to multiple second liquid storage tank.Other of reagent method for filling of the present invention change in embodiment, and second liquid storage tank can connect multiple first liquid storage tank, and second liquid storage tank can be carried out reagent perfusion by multiple first liquid storage tank.Similar, in above-mentioned change embodiment, the perfusion for the second liquid storage tank does not need the operation carrying out air pressure switching equally, decreases the time of afunction and the consumption of gas flow in filling process, has saved cost.
Above content is in conjunction with concrete embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.

Claims (12)

1. a reagent filling device, it is characterized in that: comprise at least one first liquid storage tank and at least one second liquid storage tank, described at least one first liquid storage tank connects the first outside positive pressure sources and negative source by the first pressure-gradient control valve, the first reagent interface is connected with bottom first liquid storage tank, described at least one second liquid storage tank connects the second outside positive pressure sources, the second liquid storage tank is made to remain malleation, the second reagent interface is connected with bottom second liquid storage tank, the pressure that described first positive pressure sources provides is greater than the pressure that described second positive pressure sources provides, described at least one first liquid storage tank is connected by the second fluid path control cock with at least one second liquid storage tank, described at least one first liquid storage tank is also connected to reagent container by the first fluid path control cock, when pouring into, close the second fluid path control cock, open the first pressure-gradient control valve and the first fluid path control cock, first liquid storage tank internal gas pressure is switched to from the pressure that the first positive pressure sources provides the pressure that negative source provides, described reagent container carries out reagent perfusion at least one first liquid storage tank, then, close the first fluid path control cock and the first pressure-gradient control valve, open the second fluid path control cock, the pressure that first liquid storage tank internal gas pressure is provided by negative source is switched to the pressure that the first positive pressure sources provides, described at least one first liquid storage tank pours at least one second liquid storage tank under the difference of pressure effect of the first positive pressure sources and the second positive pressure sources.
2. reagent filling device according to claim 1, it is characterized in that: described reagent filling device comprises first liquid storage tank and second liquid storage tank, described first liquid storage tank connects described first positive pressure sources and negative source by the first pressure-gradient control valve, and described second liquid storage tank connects described second positive pressure sources.
3. reagent filling device according to claim 1, it is characterized in that: described reagent filling device comprises multiple first liquid storage tank and second liquid storage tank, described multiple first liquid storage tank connects described first positive pressure sources and negative source by the first pressure-gradient control valve, and described second liquid storage tank connects described second positive pressure sources.
4. reagent filling device according to claim 1, it is characterized in that: described reagent filling device comprises first liquid storage tank and multiple second liquid storage tank, described first liquid storage tank connects described first positive pressure sources and negative source by the first pressure-gradient control valve, and described multiple second liquid storage tank connects described second positive pressure sources.
5. reagent filling device according to claim 1, it is characterized in that: described reagent filling device comprises multiple first liquid storage tank and multiple second liquid storage tank, described multiple first liquid storage tank connects described first positive pressure sources and negative source by the first pressure-gradient control valve, and described multiple second liquid storage tank connects described second positive pressure sources.
6. a reagent method for filling, comprising:
The first liquid storage tank and the second liquid storage tank that are connected by valve are provided, first liquid storage tank connects the first outside positive pressure sources and negative source by valve, the first reagent interface is connected with bottom first liquid storage tank, second liquid storage tank connects the second outside positive pressure sources, the second liquid storage tank is made to remain malleation, be connected with the second reagent interface bottom second liquid storage tank, the pressure that described first positive pressure sources provides is greater than the pressure that described second positive pressure sources provides;
There is provided the reagent container that reagent is housed, reagent container is connected to the first liquid storage tank by valve;
Reagent container carries out reagent perfusion to the first liquid storage tank;
First liquid storage tank carries out reagent perfusion to the second liquid storage tank.
7. reagent method for filling according to claim 6, is characterized in that: described reagent container carries out reagent perfusion to the first liquid storage tank and comprises:
Open the valve between the first liquid storage tank and negative source, make the first liquid storage tank inside switch to negative pressure;
Open the valve between the first liquid storage tank and reagent container, in reagent container, reagent is filled in the first liquid storage tank under vacuum suction.
8. the reagent method for filling according to claim 6 or 7, is characterized in that: described first liquid storage tank carries out reagent perfusion to the second liquid storage tank and comprises:
Close the valve between the first liquid storage tank and reagent container, the reagent perfusion of the first liquid storage tank stops;
Close the valve between the first liquid storage tank and negative source, the first liquid storage tank internal pressure switches to malleation;
Open the valve between the first liquid storage tank and the second liquid storage tank, in the first liquid storage tank, reagent is filled in the second liquid storage tank.
9. a reagent method for filling, comprising:
Multiple first liquid storage tank and multiple second liquid storage tank are provided, first liquid storage tank connects the first outside positive pressure sources and negative source by valve, the first reagent interface is connected with bottom first liquid storage tank, second liquid storage tank connects the second outside positive pressure sources, the second liquid storage tank is made to remain malleation, the second reagent interface is connected with bottom second liquid storage tank, the pressure that described first positive pressure sources provides is greater than the pressure that described second positive pressure sources provides, and is connected between the first liquid storage tank and the second liquid storage tank by valve;
There is provided reagent container, reagent container is connected by valve with the first liquid storage tank;
Reagent container carries out reagent perfusion to the first liquid storage tank;
First liquid storage tank carries out reagent perfusion to the second liquid storage tank.
10. reagent method for filling according to claim 9, is characterized in that: reagent container carries out reagent perfusion to the first liquid storage tank and comprises:
Close the valve between the first liquid storage tank and the second liquid storage tank;
Open the valve between negative source and the first liquid storage tank, make the first liquid storage tank inside be negative pressure;
Open the valve between the first liquid storage tank and reagent container, the reagent in reagent container is filled in the first liquid storage tank.
11. reagent method for filling according to claim 9 or 10, is characterized in that: the first liquid storage tank carries out reagent perfusion to the second liquid storage tank and comprises:
Close the valve between the first liquid storage tank and reagent container;
Close the valve between the first liquid storage tank and negative source;
Open the valve between the first liquid storage tank and the second liquid storage tank, the reagent in the first liquid storage tank is filled in the second corresponding liquid storage tank.
12. reagent method for filling according to claim 11, it is characterized in that: described reagent method for filling also comprises: close the valve between the first liquid storage tank and the second liquid storage tank, open the valve between the first liquid storage tank and negative source, open the valve between the first liquid storage tank and reagent container, reagent perfusion is carried out to the first liquid storage tank.
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