CN102526218A - Method for preparing Polygonum capilalum extract and Polygonum capilalum extract - Google Patents
Method for preparing Polygonum capilalum extract and Polygonum capilalum extract Download PDFInfo
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Abstract
The invention relates to a method for preparing a Polygonum capilalum extract and a Polygonum capilalum extract. The method for preparing Polygonum capilalum extract substantially comprises the following steps: (1), boiling fresh or dried aerial parts of Polygonum capitatum in water, filtering, and concentrating and drying the filtrate; and (2), extracting the dried product obtained in the step (1) with 76% to 84% ethanol, filtering, and concentrating and drying the filtrate, to obtain the Polygonum capitatum extract. The Polygonum capitatum extract can be used for preparing medicaments with effect of removing heat and dampness, medicaments for relieving stranguria by inducing diuresis, medicaments for treating infection or calculus of the urinary system, medicaments for treating pyelonephritis, medicaments with effects of removing toxic substances and removing blood stasis, medicaments with antibacterial, anti-inflammatory and analgesic effects and medicaments for treating prostatitis.
Description
Technical field
The invention belongs to the medicinal plant extract field; Be specifically related to a kind of method and Herba Polygoni Capitati extract for preparing Herba Polygoni Capitati extract, especially, said extract is obtained by following steps: get bright article of Herba Polygoni Capitati aerial parts or dry product; Decocte with water 2~4 hours; Filter, relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃, and concentrated solution spray drying or drying under reduced pressure become dry thing; Dry thing is ground into 100 order fine powders, and 80~83% ethanol extractions of 8 times of quality of fine powder reuse 1 hour filter, and filtrating is drying to obtain.
Background technology
Known Herba Polygoni Capitati (Polygonum capitatum Buch-Ham ex D.Don) is a Polygonaceae Polygonum herbaceos perennial.Effect with clearing away heat-damp and promoting diuresis, inducing diuresis for treating stranguria syndrome, treating urinary system infection clinically has significant effect.With it is that Chinese patent medicine preparation " the clear granule of pyretic stranguria " of processing of raw material got into the national essential drugs catalogue in 2002, gets into national basic medical insurance catalogue in 2004.
Herba Polygoni Capitati is the medicine commonly used of minority area, is mainly used in diseases such as pyelonephritis, urinary tract infection, inducing diuresis for treating stranguria syndrome.Relevant pharmaceutical research is rare, and several pieces of reports such as Ren Guangyou are only arranged at present.Ren Guangyou adopts rat bacterial pyelonephritis model to experimentize, and WBC in the Herba Polygoni Capitati water extract group rat urine and BLD and the apparent in view minimizing of matched group as a result shows that the Herba Polygoni Capitati water extract has certain antiinflammatory action to pyelonephritis.Ren Guangyou etc. observe dead mouse situation in 5 days with mouse peritoneal injection Escherichia coli bacteria liquid, and the matched group mortality rate is 100% as a result, and Herba Polygoni Capitati group mortality rate is respectively 20% and 50%, show that the Herba Polygoni Capitati water extract can resist the infection that escherichia coli cause.Ren Guangyou etc. give rabbit with Herba Polygoni Capitati water extract gastric infusion, the result, Herba Polygoni Capitati water extract group and matched group relatively, body temperature there was no significant difference, but can reduce the heating body temperature of the rabbit that the intravenous injection TAB causes.Ren Guangyou etc. are with Herba Polygoni Capitati water extract respectively gastric infusion rabbit, rat, with blank control group and furosemide matched group urine amount relatively.The result shows that the Herba Polygoni Capitati water extract does not have obvious diuresis to rabbit and rat.People such as Xu Yingchun adopt agar dilution to detect the external bacteriostatic activity of Herba Polygoni Capitati to 10 strain Neisseria gonorrhoeae (gonococcus), and Herba Polygoni Capitati has bacteriostatic activity to gonococcus as a result.It is 8~32g/L to the gonococcal minimal inhibitory concentration scope of 10 strains, and meansigma methods is 11.2g/L.
One Chinese patent application prospectus CN1054899A discloses a kind of miganling instant herbal medicine, syrup production technology in (one Chinese patent application numbers 90107810.7, open day on October 2nd, 1991).This production technology is to be that the raw material water decocted 30-60 minute with four seasons grass; Extracting liquid filtering concentrates, and its supernatant concentrating under reduced pressure is become cream, reuse 60-70% ethanol extraction; With the ethanol extract vacuum drying; Herba Polygoni Capitati extractum, further be mixed with electuary or syrup, it is believed that this has detoxifcation, dissipating blood stasis, diuresis, treating stranguria effect.
Recorded the Chinese patent medicine preparation of " the clear granule of pyretic stranguria " by name in the Ministry of Health of the People's Republic of China " drug standard one Chinese traditional patent formulation preparation " (the 17) that Ministry of Health of the People's Republic of China's committee of pharmacopeia was compiled in 1998, it is processed through twice after-filtration of Herba Polygoni Capitati decocte with water concentrated.
CN 1481832A (one Chinese patent application number 02129686.3; Open day on March 17th, 2004) and CN 1483466A (one Chinese patent application number 03146381.9; Open day on March 24th, 2004) Herba Polygoni Capitati extract is disclosed; It is prepared by following steps basically: a. is decocted at twice by the aquatic foods article of Herba Polygoni Capitati herb or dry product water or divides reflux, extract, two to three times with alcohol-water mixture, each 1-2 hour, merges decoction liquor; Relative density during filtering and concentrating to 20 ℃ is 1.2, and spray drying or drying under reduced pressure obtain; Perhaps, b. carries medicinal residues by Herba Polygoni Capitati herb and water thereof and obtains through carbon dioxide supercritical fluid extraction.It is believed that this extract can be used for antibiotic, antiinflammatory, analgesia, diuresis, treatment urinary system calculus, treatment pyelonephritis and prostatitis.
Although many reports about the preparation Herba Polygoni Capitati extract are arranged at present, yet those skilled in the art expect still to obtain more efficiently product from Herba Polygoni Capitati.
Summary of the invention
The present invention seeks to expectation and obtain more efficiently product from Herba Polygoni Capitati.The inventor finds use and earlier Herba Polygoni Capitati is carried the concentrate drying article that obtain through water, and then with these concentrate drying article of certain density ethanol extraction, the extract that obtains to have the effect of desirable aspect biological activity.The present invention is based on this discovery and be accomplished.
First aspect present invention provides a kind of method for preparing Herba Polygoni Capitati extract, and it consists essentially of following steps:
(1) with bright article of Herba Polygoni Capitati aerial parts or dry product decocte with water, filters, make filtrating concentrating, dry;
(2) with the dry thing of step (1) gained with 76~84% ethanol extractions, filter, making filtrating concentrated, dry, promptly get.
According to the method for first aspect present invention, in the wherein said step (1), doubly measure water extraction 1-2 time of (preferred 5-13 doubly measures, and more preferably 5-10 doubly measures), each 1-4 hour (preferred 1-3 hour perhaps preferably 1-2 hour) with the 5-15 of Herba Polygoni Capitati dry product or bright article.
According to the method for first aspect present invention, in the wherein said step (1), relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃.
According to the method for first aspect present invention, in the wherein said step (1), it is dry that filtrating concentrates back mode spray-dried or drying under reduced pressure.
According to the method for first aspect present invention, in the wherein said step (2), used ethanol is 78~84% ethanol, more preferably 80~84% ethanol, for example about 82% ethanol.
According to the method for first aspect present invention, in the wherein said step (2), the dry thing of step (1) gained is ground into the fine powder of 60-120 order (preferred 80-120 order, for example about 100 orders), and then carries out ethanol extraction.
According to the method for first aspect present invention, in the wherein said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.In one embodiment, extraction time can be 0.5~5 hour (for example 1-4 hour, for example 1-3 hour).
Second aspect present invention provides a kind of Herba Polygoni Capitati extract, and it is prepared by the method that may further comprise the steps basically:
(1) with bright article of Herba Polygoni Capitati aerial parts or dry product decocte with water, filters, make filtrating concentrating, dry;
(2) with the dry thing of step (1) gained with 76~84% ethanol extractions, filter, making filtrating concentrated, dry, promptly get.
Herba Polygoni Capitati extract according to first aspect present invention; In the wherein said step (1); 5-15 with Herba Polygoni Capitati dry product or bright article doubly measures water extraction 1-2 time of (preferred 5-13 doubly measures, and more preferably 5-10 doubly measures), each 1-4 hour (preferred 1-3 hour perhaps preferably 1-2 hour).
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (1), relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (1), it is dry that filtrating concentrates back mode spray-dried or drying under reduced pressure.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), used ethanol is 78~84% ethanol, more preferably 80~84% ethanol, for example about 82% ethanol.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), the dry thing of step (1) gained is ground into the fine powder of 60-120 order (preferred 80-120 order, for example about 100 orders), and then carries out ethanol extraction.
According to the Herba Polygoni Capitati extract of first aspect present invention, in the wherein said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.In one embodiment, extraction time can be 0.5~5 hour (for example 1-4 hour, for example 1-3 hour).
Third aspect present invention provides the said Herba Polygoni Capitati extract of second aspect present invention in the medication of preparation clearing away heat-damp and promoting diuresis; The inducing diuresis for treating stranguria syndrome medication; Urinary system infection or calculus medication, pyelonephritis medication, detoxifcation, dissipating blood stasis medication; Antibiotic, antiinflammatory, analgesia medication, the purposes in the medicine of prostatitis medication.
Fourth aspect present invention provides a kind of pharmaceutical composition, wherein comprises described Herba Polygoni Capitati extract of the arbitrary embodiment of second aspect present invention and optional pharmaceutically acceptable carrier.
According to the pharmaceutical composition of fourth aspect present invention, it is the dosage form of oral or drug administration by injection.In one embodiment, said pharmaceutical composition is the form of tablet, capsule, granule, pill, oral solutions, injection (liquid drugs injection and/or powder pin) etc.
Arbitrary technical characterictic that arbitrary embodiment had of the arbitrary aspect of the present invention or this arbitrary aspect is suitable for arbitrary embodiment of other arbitrary embodiment or other arbitrary aspect equally; As long as they can be not conflicting; Certainly at where applicable each other, necessary words can be done suitably to modify to individual features.Do further to describe with characteristics to various aspects of the present invention below.
All documents that the present invention quoted from, their full content is incorporated this paper by reference into, and if the expressed implication of these documents and the present invention when inconsistent, be as the criterion with statement of the present invention.In addition; Various terms and phrase that the present invention uses have the general sense of well known to a person skilled in the art; Nonetheless; The present invention still hopes at this more detailed explanation and explanation to be done in these terms and phrase, and term of mentioning and phrase are as the criterion with the implication that the present invention was explained if any inconsistent with known implication.
In the present invention, the inventive method obtains " Herba Polygoni Capitati extract ", and this term also can be described as " Herba Polygoni Capitati active component " of the present invention or " Herba Polygoni Capitati effective site " of the present invention.
In the methods of the invention; Obtain water extract through step (1); The operating condition of this step need not be done special restriction, and reason is that the inventor finds that in step (2), adopting the extraction solvent of unique concentration is ethanol water, the effect of the pharmacodynamics raising of desirable occurs.
The object of the invention is to overcome the deficiency of prior art, a kind of effective Herba Polygoni Capitati effective content of anti inflammation (effective site) is provided and uses.
Drug action focuses on to Herba Polygoni Capitati in invention; Understand fully that its effect characteristics screen the drug action of the different extract parts of Herba Polygoni Capitati simultaneously, adopt the sample of the different extract parts of Herba Polygoni Capitati to carry out the screening of Herba Polygoni Capitati active component (effective site) with the drug action characteristics through antiinflammatory, analgesia, antibiotic pharmacological evaluation.
In one embodiment, invention realizes above-mentioned purpose through following technical scheme:
Invention provides a kind of Herba Polygoni Capitati active component (effective site), is to be obtained by following steps:
(1) get bright article of Herba Polygoni Capitati aerial parts or dry product, decocte with water 2~4 hours is filtered, and relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃, and concentrated solution spray drying or drying under reduced pressure become dry thing;
(2) dry thing is ground into 100 order fine powders, and 76~84% ethanol extractions of 8 times of quality of fine powder reuse 1 hour filter, and filtrating is drying to obtain.Ethanol in the filtrating can reclaim reuse.
In one embodiment, the addition of the described water of step (1) is Herba Polygoni Capitati dry product or bright article 10-13 a times; Decocte with water is carried out at twice, each 1~2 hour.
In one embodiment, the described ethanol extraction of step (2) carries out at twice, and each 0.5 hour, described being extracted as stirred extraction or ultrasonic extraction.
In one embodiment, in the alcoholic acid leaching process of step (2), alcoholic acid mass fraction is an important factor, is related to the medicinal effects of the effective site of being extracted.Concentration of alcohol is low, and Herba Polygoni Capitati medicated powder adheres to easily, causes and extracts not exclusively, the commercial production difficulty.The high polar component of concentration of alcohol extracts and reduces, and influences drug effect.The highly preferred concentration of alcohol of the present invention is 80~84%, is more preferably 82%.
A kind of preferred version of the present invention below is provided, and the Herba Polygoni Capitati active component is to be obtained by following method: decocted with 13 times of water (W/W) by the Herba Polygoni Capitati dry product, can be divided into secondary and decoct; Each 1~2 hour; Filter, merging filtrate, relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃; Concentrated solution spray drying or drying under reduced pressure, drying under reduced pressure thing are ground into 100 order fine powders.8 times of (W/W) 82% ethanol extractions of dry fine powder reuse divide second extraction (ultrasonic extraction), and each 0.5 hour, filter, merging filtrate reclaims ethanol, and residue dried promptly gets.
Herba Polygoni Capitati active component provided by the present invention (effective site); Xylol causes the influence experiment of mice ear; Press 5g Herba Polygoni Capitati dry product extract obtained/kg body weight gastric infusion, continuous 5 days, test behind the last administration 60min; Significant antiinflammatory action is arranged, and the highest inflammation suppression ratio can reach 46.08%; Cause the pain experiment for the mice hot plate, can prolong the mice hot plate and add foot reaction latent time (comparing P<0.05 with matched group), the highest time-delay rate can reach 16.57%.
This Herba Polygoni Capitati active component can become various common dosage forms and slow releasing preparation, controlled release preparation, targeting preparation etc. with acceptable adjuvant combined preparation in the medicine manufacturing.
Water is carried although the Herba Polygoni Capitati medicine adopts, the technology of alcohol extraction, decocting in water alcohol extraction is comparatively known way; But water breakthrough (boiling) promotion is not dry, pulverizes, the technology of certain concentration ethanol extraction again; Compared with prior art, the present invention has the different of following beneficial effect and character.
The present invention extracts with decocting in water earlier, and the method that obtains active ingredient and then concentrate with polar solvent (ethanol of certain concentration) can make antiinflammatory action than the obvious enhancing of water extract.The extract of extract of the present invention and extraction process by water relatively can improve its antiphlogistic effects in highly significant ground, and its experimental result is as shown in the table:
Group | Dosage (g/kg) | n | Swelling degree (mg) | Suppression ratio (%) |
Matched group | ?- | 10 | 6.8±3.01 | |
The Herba Polygoni Capitati water extract | 2.5 | 10 | 5.3±1.35 | 22.06 |
The Herba Polygoni Capitati water extract | 5 | 10 | 4.8±1.55 | 29.41 |
Extract of the present invention | 2.5 | 10 | 4.4±1.26 * | 35.29 |
Extract of the present invention | 5 | 9 | 3.67±1.00 ** | 46.08 |
Annotate: compare with matched group:
*P<0.05
*P<0.01
In the last table; The method for distilling that " extract of the present invention " adopts is: the Herba Polygoni Capitati dry product is divided into secondary with 13 times of water (W/W) decocts; Each 1.5 hours, filter merging filtrate; Relative density was 1.1 when filtrating was concentrated into 20 ℃, and the concentrated solution spray drying gets the above fine powder of 100 orders (this fine powder is the reagent of going up table " Herba Polygoni Capitati water extract ").8 times of (W/W) 82% ethanol extractions of dry fine powder reuse divide second extraction (ultrasonic extraction), and each 0.5 hour, filter, merging filtrate reclaims ethanol, and residue dried promptly gets (as the reagent of " extract of the present invention " in the last table).
In the present invention, during the dosage representing to test with g/kg, as do not have other explanation, be meant that every kg the weight of animals gives the corresponding reagent weight that (extract for example of the present invention) amounted to into the Herba Polygoni Capitati dry product.
The specific embodiment
In order more to be expressly understood technology contents of the present invention, the special following examples of lifting specify, but embodiment of the present invention is not limited thereto.
A, extract prepare routine part
Preparation example 1: Herba Polygoni Capitati water extract
Get Herba Polygoni Capitati aerial parts dry product 10Kg, wash, add 10 times of water gagings (W/W); Soaked 1 hour, heated and boiled 1 hour is filtered; Filtering residue adds 6 times of water gagings again and boiled 1 hour, filters merging filtrate; Relative density reached 1.3 when filtrating was concentrated into 20 ℃, and the concentrated solution spray drying gets dry fine powder 1.03Kg.
Preparation example 2: the preparation of extract of the present invention
Get dry fine powder (the about 100 orders) 0.5Kg of the routine 1 method gained of preparation, divide twice ultrasonic to extract each 0.5 hour with 8 times of (W/W) 82% ethanol; Filter, merging filtrate reclaims ethanol; The residue drying under reduced pressure gets dry thing 0.047Kg, and this extract is used for the reagent of hereinafter Test Example part test.
Prepare routine 2a: the preparation of extract of the present invention
In addition, with reference to above example 2 the method that prepare, use 6 times of (W/W) 82% ethanol, 10 times of (W/W) 82% ethanol to extract respectively and supersound process 2 times each 5 hours, extract yield is basic identical with preparation routine 2; Carry out the part test of hereinafter Test Example equally with extract obtained again; The result shows the extract obtained BA basic identical (differing less than 10%) of 8 times of (W/W) 82% ethanol extractions of usefulness with preparation example 2; Be illustrated under the condition of same concentrations extracting solution, extraction conditions such as extracting solution consumption and alcohol processing time do not have influence to the activity of extract.
Preparation example 3: the preparation of extract of the present invention
Get dry fine powder (the about 80 orders) 0.5Kg of the routine 1 method gained of preparation, with 8 times (W/W) 76% alcohol reflux 1 time, each 1 hour, filter, merging filtrate reclaims ethanol, and the residue drying under reduced pressure gets dry thing 0.067Kg.
Preparation example 4: the preparation of extract of the present invention
Get dry fine powder (the about 120 orders) 0.5Kg of the routine 1 method gained of preparation, divide twice ultrasonic to extract with 8 times of (W/W) 84% ethanol, each 3 hours, filter, merging filtrate reclaims ethanol, and the residue drying under reduced pressure gets dry thing 0.053Kg.
Preparation example 5: the preparation of extract of the present invention
Get the bright article 20Kg of Herba Polygoni Capitati aerial parts, wash, add 7 times of water gagings (W/W); Soaked 1 hour, heated and boiled 1 hour is filtered; Filtering residue adds 5 times of water gagings again and boiled 1 hour, filters merging filtrate; Relative density reached 1.1 when filtrating was concentrated into 20 ℃, and the concentrated solution spray drying gets dry fine powder 0.63Kg.
Get dry fine powder (the about 100 orders) 0.5Kg of above preparation, carry out alcohol extraction with reference to the method for preparing example 4 then, difference is that used ethanol is 80% ethanol, gets dry thing 0.055Kg.
Reference examples 1: the preparation of reference extract
With reference to the method for preparing example 2, difference is that used ethanol is 70%, gets dry thing 0.075Kg.
Reference examples 2: the preparation of reference extract
With reference to the method for preparing example 2, difference is that used ethanol is 60%, gets dry thing 0.083Kg.
Reference examples 3: the preparation of reference extract
With reference to the method for preparing example 2, difference is that used ethanol is 90%, gets dry thing 0.033Kg.
Reference examples 4: the preparation of reference extract
With reference to the method for preparing example 5, difference is that the used ethanol of alcohol extraction step is 72%, gets dry thing 0.081Kg.
Reference examples 5: the preparation of reference extract
With reference to the method for preparing example 5, difference is that the used ethanol of alcohol extraction step is 88%, gets dry thing 0.036Kg.
B, pharmacodynamics test example part
Test Example 1: the experiment of mice caused by dimethylbenzene xylene ear swelling, the antiphlogistic effects of investigation Herba Polygoni Capitati extract
Animal: SPF level Kunming mouse, body weight 18~22g, male and female dual-purpose.
Reagent, dosage and medication: as reagent, dosage is that to give the weight that corresponding reagent amounts to into the Herba Polygoni Capitati dry product be 5g to every kg the weight of animals with the routine sample of various preparations of preceding text " A, extract prepare routine part " gained and reference examples sample.Each reagent is faced with preceding and is suspended to the concentration that is equivalent to every each gastric infusion 0.4ml of animal with normal saline, is provided with simultaneously to irritate the matched group of stomach with the volume normal saline.
Test method: mice male and female dual-purpose, random packet, 10 animals of each reagent group; Successive administration 5 days behind the last administration 60min, only is coated with auris dextra with xylene 0.1ml/ and causes inflammation, puts to death mice behind the 30min, two auricles about getting with the card punch of diameter 6mm.Use scales/electronic balance weighing immediately, with two auricle weight differences as swelling degree (mg), with the suppression ratio (%) of each reagent group of computes:
Wherein swelling degree (mg)=auris dextra sheet weight-left auricle is heavy
The result carries out statistical analysis.Experimental result is seen table 1.
Suppression ratio is carried out classification, and suppression ratio is lower than 10% and is the A level, and suppression ratio 10~20% is the AA level, and suppression ratio 20~30% is the AAA level, and suppression ratio 30~40% is the AAAA level, and suppression ratio 40~50% is the AAAAA level, and suppression ratio is the AAAAAA level greater than 50%.
Table 1: different Herba Polygoni Capitati xylol causes the influence of mice ear
Reagent | The suppression ratio classification |
Preparation example 1 | AAA * |
Preparation example 2 | AAAA ** |
Preparation example 3 | AAAAA ** |
Preparation example 4 | AAAAA ** |
Preparation example 5 | AAAAAA ** |
Reference examples 1 | AA |
Reference examples 2 | AAA * |
Reference examples 3 | A |
Annotate: swelling degree (mg), compare with the solvent control animals:
*P<0.05,
*P<0.01.In addition, the result of reference examples 4 and reference examples 5 reagents is respectively AA and A.
Test Example 2: the heat-resisting hot plate reaction experiment of mice, the analgesic effect of investigation Herba Polygoni Capitati extract
Animal, reagent, dosage and medication are identical with Test Example 1.
Test method: mice male and female dual-purpose, random packet, 10 animals of each reagent group; Successive administration 5 days behind the last administration 60min, is put into intelligent hot-plate instrument with each group mice, and the observed and recorded mice is added foot reaction latent time, and each is organized mice measurement 2 times (each about 15min at interval) and gets its meansigma methods.Experimental result is seen table 2.
Carry out classification to adding foot reaction latent time, latent time is lower than 6 seconds and is the A level, and latent time 6~7 seconds was the AA level, and latent time 7~8 seconds was the AAA level, and latent time 8~9 seconds was the AAAA level, and latent time was the AAAAA level greater than 9 seconds.
Table 2: different Herba Polygoni Capitati is to the influence of the heat-resisting hot plate reaction of mice
Reagent | Add the classification of foot reaction latent time |
Preparation example 1 | AA |
Preparation example 2 | AAAA ** |
Preparation example 3 | AAA * |
Preparation example 4 | AAAA ** |
Preparation example 5 | AAAA ** |
Reference examples 1 | AA |
Reference examples 2 | A |
Reference examples 3 | A |
The solvent matched group | A |
Annotate: add foot reaction latent time (second), compare with the solvent control animals:
*P<0.05
*P<0.01.In addition, reference examples 4 is the A level with reference examples 5 reagent latent time classification results.
Embodiment 3: the antibacterial experiment of different Herba Polygoni Capitati extract
Adopt conventional antibacterial tests method; Use the MH broth bouillon that bacillus pyocyaneus, escherichia coli M421C1ST, dysentery bacterium, staphylococcus aureus, Hemolytic streptococcus are made an experiment; As reagent, test their sensitivity with the routine sample of various preparations of preceding text " A, extract prepare routine part " gained and reference examples sample to these cells.
The result shows that it is responsive to bacillus pyocyaneus, escherichia coli to prepare routine 1 sample, MIC>20mg/ml; Each sample of reference examples 1-3 is insensitive to bacillus pyocyaneus, escherichia coli, dysentery bacterium, staphylococcus aureus, to streptococcic MIC>10mg/ml; Prepare each sample of routine 2-5 to the MIC of five kinds of antibacterials between 1-4mg/ml, to the MIC=2.5mg/ml of individual cells.
Claims (10)
1. prepare the method for Herba Polygoni Capitati extract, it consists essentially of following steps:
(1) with bright article of Herba Polygoni Capitati aerial parts or dry product decocte with water, filters, make filtrating concentrating, dry;
(2) with the dry thing of step (1) gained with 76~84% ethanol extractions, filter, making filtrating concentrated, dry, promptly get.
2. according to the method for claim 1, it is characterized in that:
In the said step (1), the water extraction of doubly measuring with the 5-15 of Herba Polygoni Capitati dry product or bright article 1-2 time, 1-4 hour at every turn; And/or
In the said step (1), relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃.
3. according to the method for claim 1, it is characterized in that:
In the said step (2), used ethanol is 80~84% ethanol;
In the said step (2), the dry thing of step (1) gained is ground into 60-120 purpose fine powder, and then carries out ethanol extraction; And/or
In the said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.
4. Herba Polygoni Capitati extract, it is prepared by the method that may further comprise the steps basically:
(1) with bright article of Herba Polygoni Capitati aerial parts or dry product decocte with water, filters, make filtrating concentrating, dry;
(2) with the dry thing of step (1) gained with 76~84% ethanol extractions, filter, making filtrating concentrated, dry, promptly get.
5. according to the Herba Polygoni Capitati extract of claim 4, it is characterized in that:
In the said step (1), the water extraction of doubly measuring with the 5-15 of Herba Polygoni Capitati dry product or bright article 1-2 time, 1-4 hour at every turn;
In the said step (1), relative density was 1.1~1.3 when filtrating was concentrated into 20 ℃; And/or
In the said step (1), it is dry that filtrating concentrates back mode spray-dried or drying under reduced pressure.
6. according to the Herba Polygoni Capitati extract of claim 4, it is characterized in that:
In the said step (2), used ethanol is 80~84% ethanol;
In the said step (2), the dry thing of step (1) gained is ground into 60-120 purpose fine powder, and then carries out ethanol extraction; And/or
In the said step (2), use alcoholic solution under the condition of the condition of reflow treatment or ultrasonic Treatment, to extract.
7. each said Herba Polygoni Capitati extract of claim 4-6 is in the medication of preparation clearing away heat-damp and promoting diuresis, inducing diuresis for treating stranguria syndrome medication, urinary system infection or calculus medication; The pyelonephritis medication; Detoxifcation, dissipating blood stasis medication, antibiotic, antiinflammatory, analgesia medication, the purposes in the medicine of prostatitis medication.
8. a pharmaceutical composition wherein comprises each described Herba Polygoni Capitati extract of claim 4-6 and optional pharmaceutically acceptable carrier.
9. the pharmaceutical composition of claim 8, it is the dosage form of oral or drug administration by injection.
10. the pharmaceutical composition of claim 8, it is the form of tablet, capsule, granule, pill, oral solutions, injection (liquid drugs injection and/or powder pin) etc.
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