CN102524140A - Method for carrying out anesthesia and blood drawing on Takifugu obscurus - Google Patents
Method for carrying out anesthesia and blood drawing on Takifugu obscurus Download PDFInfo
- Publication number
- CN102524140A CN102524140A CN2012100512848A CN201210051284A CN102524140A CN 102524140 A CN102524140 A CN 102524140A CN 2012100512848 A CN2012100512848 A CN 2012100512848A CN 201210051284 A CN201210051284 A CN 201210051284A CN 102524140 A CN102524140 A CN 102524140A
- Authority
- CN
- China
- Prior art keywords
- blood drawing
- anesthesia
- fugu obscurus
- obscurus
- blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a method for carrying out anesthesia and blood drawing on Takifugu obscurus, which comprises the following operating steps of: firstly, adding pood water into a container; regulating the water temperature into 15 to 20 DEG C; adding clove oil into the container to prepare anesthetic solution with the weight ratio of the water to the clove oil of 99.98:0.02; placing the Takifugu obscurus into the anesthetic solution, wherein the anesthesia time is 2 to 5 minutes; drawing blood by a syringe at the caudal vein, wherein the blood drawing time is 1 to 1.2 minutes; and after drawing blood, transferring the Takifugu obscurus into a temporary culturing pood for revival. According to the invention, aiming at the physiological property of the Takifugu obscurus, the method is successfully used for carrying out anesthesia and blood drawing on the Takifugu obscurus, has low cost, is simple and convenient to operate, has small damage to the fish body and has a large blood drawing quantity.
Description
Technical field
The present invention relates to a kind of anesthesia blood drawing method of fugu obscurus, belong to technical field of aquaculture.
Background technology
Fugu obscurus (
Takifugu Obscurus) be commonly called as the river Puffer, be under the jurisdiction of Osteichthyes (Osteichthyes) Tetraodontiformes (Tetraodontiformes) Molidae (Tetraodontidae) Fugu and belong to (Fugu), be sea, river migration fishes, have the good reputation of " king in the fish "; The fugutoxin that extracts from its internal organ is a kind of fatal biotoxin, also is simultaneously a kind of arcotic and drug rehabilitation good medicine of strong effect, has very high economic worth.Because market prospects are good, since the artificial propagation success nineties in last century, the artificial increasing cultivation of river Puffer is developed rapidly.In recent years, because inbreeding, manual work is introduced a fine variety and nonstandard propagation is released to be caused germplasm is degenerated and the germplasm mixed questions is on the rise, fugu obscurus blastogenesis Study on Diversity becomes the research focus gradually.In the research of germplasm molecular genetic, many times all need gather enough blood samples.Because the fugu obscurus body surface smooth does not have scale; The high build of body fat content is fat, and blood drawing macrura reevesii body is struggled, and adopts the conventional tail venous blood collection not only to be difficult to find the tail vein; And the fish body is very easily injured, therefore presses for and finds a kind of blood-sampling method of fugu obscurus efficiently.
Summary of the invention
The present invention seeks to provide to the defective that prior art exists a kind of anesthesia blood drawing method of fugu obscurus, the mixed solution that adopts caryophyllus oil and water is as anesthetic solution, and the primary sterilization syringe is drawn blood at fish tail vein place.
The present invention is for realizing above-mentioned purpose, and adopt following technical scheme: a kind of anesthesia blood drawing method of fugu obscurus comprises the steps:
A, anesthesia
In container, add earlier pond water, regulating water temperature is 15～20 ℃; In container, add caryophyllus oil, being mixed with water and caryophyllus oil weight ratio is the anesthetic solution of 99.98:0.02; Fugu obscurus is put into anesthetic solution, and anesthesia duration is: 2～5 minutes;
B, blood drawing
Treat after tripe is turned in fugu obscurus anesthesia dish to be put in its taking-up, the fugu obscurus afterbody is lifted slightly, in its tail vein place blood drawing, the blood drawing time is 1～1.2 minute with syringe;
C, temporary the supporting of reviving
Changing fish over to storage pond, holding pond after the blood drawing revives.
As preferably, said syringe is the primary sterilization syringe of 2ml;
As preferably, the body weight of said fugu obscurus is 110～150g;
As preferably, the concentration of eugenol is greater than 85% in the said caryophyllus oil.
Beneficial effect of the present invention: the physiological property that the present invention is directed to fugu obscurus is successfully anaesthetized, drawn blood it, and cost is low, and is easy and simple to handle, and is little to the injury of fish body, and the blood drawing amount is many; Shown the advantage that it is efficient, convenient, workable.
Embodiment
Further describe below in conjunction with specific embodiment:
Embodiment one:
The anesthesia blood drawing method of a kind of fugu obscurus of the present invention, adopt following operating procedure:
In the foster temporarily case of the plastics of 80*80*60cm, add pond water earlier, regulating water temperature is 15～17 ℃, adds commercially available caryophyllus oil (concentration of eugenol is greater than 85%) to supporting case temporarily, and being mixed with water and caryophyllus oil weight ratio is the 99.98:0.02 anesthetic solution; With 12 urosomes heavily is that the fugu obscurus of 110～120g is put into anesthetic solution, anaesthetizes 3-3.5 minutes, and promptly visible fugu obscurus side is fallen in water; Rapidly fugu obscurus is pulled out from anesthesia liquid, used primary sterilization syringe at the tail vein place blood drawing of capacity as 2ml, the blood drawing time is 1 minute, and the blood drawing amount is 1ml; Change fish over to storage pond, holding pond after the blood drawing, after 6～7 minutes, fugu obscurus recovers normally to move about.
Embodiment two:
The anesthesia blood drawing method of a kind of fugu obscurus of the present invention, adopt following operating procedure:
In the foster temporarily case of the plastics of 80*80*60cm, add pond water earlier, regulating water temperature is 16～18 ℃, supports to temporarily to add commercially available caryophyllus oil (concentration of eugenol is greater than 85%) in the case, and being mixed with water and caryophyllus oil weight ratio is the 99.98:0.02 anesthetic solution; With 10 urosomes heavily is that the fugu obscurus of 120～130g is put into anesthetic solution, anaesthetizes 3.5～4 minutes, and visible fugu obscurus side is fallen in water; Rapidly fugu obscurus is pulled out from anesthesia liquid, used primary sterilization syringe at the tail vein place blood drawing of capacity as 2ml, the blood drawing time is 1 minute, and the blood drawing amount is 1ml; Change fish over to storage pond, holding pond after the blood drawing, after 5～6 minutes, fugu obscurus recovers normally to move about.
Embodiment three:
The anesthesia blood drawing method of a kind of fugu obscurus of the present invention, adopt following operating procedure:
In the foster temporarily case of the plastics of 80*80*60cm, add pond water earlier, regulating water temperature is 16～19 ℃, supports to temporarily to add commercially available caryophyllus oil (concentration of eugenol is greater than 85%) in the case, and being mixed with water and caryophyllus oil weight ratio is the 99.98:0.02 anesthetic solution; With 8 urosomes heavily is that the fugu obscurus of 130～150g is put into anesthetic solution, anaesthetizes 4～5 minutes, and promptly visible fugu obscurus side is fallen in water; Rapidly fugu obscurus is pulled out from anesthesia liquid, used primary sterilization syringe at the tail vein place blood drawing of capacity as 2ml, the blood drawing time is 1 minute, and the blood drawing amount is 1.5ml; Change fish over to storage pond, holding pond after the blood drawing, after 4～6 minutes, fugu obscurus recovers normally to move about.
Claims (4)
1. the anesthesia blood drawing method of a fugu obscurus is characterized in that, comprises the steps:
A, anesthesia
In container, add earlier pond water, regulating water temperature is 15～20 ℃; In container, add caryophyllus oil, being mixed with water and caryophyllus oil weight ratio is the anesthetic solution of 99.98:0.02; Fugu obscurus is put into anesthetic solution, and anesthesia duration is: 2～5 minutes;
B, blood drawing
Treat after tripe is turned in fugu obscurus anesthesia dish to be put in its taking-up, the fugu obscurus afterbody is lifted slightly, in its tail vein place blood drawing, the blood drawing time is 1～1.2 minute with syringe;
C, temporary the supporting of reviving
Changing fish over to storage pond, holding pond after the blood drawing revives.
2. the anesthesia blood drawing method of a kind of fugu obscurus according to claim 1 is characterized in that, said syringe is the primary sterilization syringe of 2ml.
3. the anesthesia blood drawing method of a kind of fugu obscurus according to claim 1 is characterized in that, the body weight of said fugu obscurus is 110～150g.
4. the anesthesia blood drawing method of a kind of fugu obscurus according to claim 1 is characterized in that, the concentration of eugenol is greater than 85% in the said caryophyllus oil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100512848A CN102524140A (en) | 2012-03-01 | 2012-03-01 | Method for carrying out anesthesia and blood drawing on Takifugu obscurus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100512848A CN102524140A (en) | 2012-03-01 | 2012-03-01 | Method for carrying out anesthesia and blood drawing on Takifugu obscurus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102524140A true CN102524140A (en) | 2012-07-04 |
Family
ID=46333193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100512848A Pending CN102524140A (en) | 2012-03-01 | 2012-03-01 | Method for carrying out anesthesia and blood drawing on Takifugu obscurus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102524140A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053452A (en) * | 2012-12-26 | 2013-04-24 | 广西大学 | Out-of-water transportation method by using carbon dioxide for anaesthetizing golden pompano and tilapias |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238801A (en) * | 2008-03-05 | 2008-08-13 | 中国水产科学研究院淡水渔业研究中心 | Anaesthesia method of tilapia |
| CN101259167A (en) * | 2008-04-14 | 2008-09-10 | 中国水产科学研究院淡水渔业研究中心 | Carp anaesthesia method |
-
2012
- 2012-03-01 CN CN2012100512848A patent/CN102524140A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238801A (en) * | 2008-03-05 | 2008-08-13 | 中国水产科学研究院淡水渔业研究中心 | Anaesthesia method of tilapia |
| CN101259167A (en) * | 2008-04-14 | 2008-09-10 | 中国水产科学研究院淡水渔业研究中心 | Carp anaesthesia method |
Non-Patent Citations (4)
| Title |
|---|
| C. A. WOODY, J. NELSON, K. RAMSTAD: "Clove oil as an anaesthetic for adult sockeye salmon: field trials", 《JOURNAL OF FISH BIOLOGY》 * |
| 张富林; 吕世明; 韩俊伟: "丁香酚对鲤的麻醉作用", 《山地农业生物学报》 * |
| 梁政远; 安丽娜; 董在杰; 缪凌鸿; 徐跑; 谢庄: "丁香油对罗非鱼的麻醉作用及其对血液指标和激素水平的影响", 《上海海洋大学学报》 * |
| 詹松文; 徐宏; 李向东: "暗纹东方鲀养殖技术", 《渔业致富指南》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103053452A (en) * | 2012-12-26 | 2013-04-24 | 广西大学 | Out-of-water transportation method by using carbon dioxide for anaesthetizing golden pompano and tilapias |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105687244B (en) | A kind of preparation, preparation method and its application | |
| CN107090431A (en) | A kind of active mesenchymal stem cell injection for being used to be transfused diabetes | |
| CN204272963U (en) | A kind of ecological chlorella circulatory system purifying air and oxygen supply in fishpond | |
| CN102524140A (en) | Method for carrying out anesthesia and blood drawing on Takifugu obscurus | |
| CN104430085A (en) | Artificial reproduction method for Triplophysa yarkandensis | |
| CN105248355B (en) | A kind of transportation resources of star column | |
| CN103505415B (en) | Medium and long chain fat emulsion injection and preparation method thereof | |
| CN102228685A (en) | Preparation method of iridovirus cell inactivated vaccine for Chinese giant salamanders, and application method thereof | |
| CN102217589A (en) | Perfusion preservation device for liver | |
| CN111281884A (en) | Method for reducing insulin dosage of diabetic patient by using stem cell therapy | |
| CN101703013B (en) | Biological engineering method of squids | |
| CN202050840U (en) | Liver perfusion and preservation device | |
| CN104094878A (en) | Method for artificially hatching loaches | |
| CN203302153U (en) | Loach fixing net cover | |
| CN103947587A (en) | Anesthesia method for northern pike | |
| CN103609512A (en) | Method for removing bubbles in a pond | |
| CN103283658A (en) | Loach fixing net cover | |
| CN203988797U (en) | A kind of upper limb hand support | |
| CN106879510B (en) | The naked induced spawning method for splitting buttocks fish in Lhasa | |
| CN105613424A (en) | Simulated ecological breeding method for soft-shelled turtles | |
| CN109169418A (en) | A method of removing epinephelus lanceolatus fish parasitism fish leech | |
| ES2553590T3 (en) | Method of repair and renal regeneration and treatment of diabetic nephropathy | |
| CN102824226B (en) | A kind of preparation method of spermatogonial stem cell transplantation receptor | |
| CN203723270U (en) | Fish tank humidifier | |
| CN103141431A (en) | Artificial method for inducing natural spawning and incubation of xenopus laevis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120704 |