CN102517249A - Culture medium for improving germination and growth of Luntai white apricot blossom pollen - Google Patents
Culture medium for improving germination and growth of Luntai white apricot blossom pollen Download PDFInfo
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- CN102517249A CN102517249A CN2011104204470A CN201110420447A CN102517249A CN 102517249 A CN102517249 A CN 102517249A CN 2011104204470 A CN2011104204470 A CN 2011104204470A CN 201110420447 A CN201110420447 A CN 201110420447A CN 102517249 A CN102517249 A CN 102517249A
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Abstract
The invention relates to a culture medium for improving germination and growth of Luntai white apricot blossom pollen. The culture medium is prepared by respectively adding gibberellins, heteroauxin, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid or 6-benzyl adenine by taking agar, saccharose and boracic acid as basic culture medium components, and culture conditions comprise culture temperature and a pH value. According to the culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen, the technical problems that the germination rate of the pollen is low and the growth of a pollen tube is slow are solved, and an effective path for improving the germination rate is found for Luntai white apricot blossoms. According to the culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen, the germination and the growth of the Luntai white apricot blossom pollen are increased. The culture medium for improving the germination and the growth of the Luntai white apricot blossom pollen has simplicity and convenience in operation, low cost, a short period and high germination rate and has a wide application prospect in stone fruit tree biology.
Description
Technical field
The present invention relates to fruit tree pollination fertilization technical field, be specifically related to a kind of substratum that improves white apricot pollen germination of Luntai and growth.
Background technology
Apricot (Armeniaca vulgaris Lam) is a defoliation small arbor, the Rosaceae, Prunus; Apricot really contains nutritive ingredients such as abundant vitamin A, vitamins C, carbohydrate, Hydrocerol A, oxysuccinic acid; The sweet acid of nature and flavor, tepor promote the production of body fluid to quench thirst so have, clearing heat and detoxicating characteristics; Certain health care and protective effect on cancer risk are arranged, eat the still edible favor that all enjoys people in processing back raw.China's apricot is distributed widely in ground such as Hebei, Beijing, Henan, Shandong, Shanxi, Shaanxi, Gansu, Xinjiang, and more than 2000 kind arranged, and wherein Xinjiang has 117 kinds, and is the most famous with the Xiao Bai apricot.The white apricot of Luntai (new S-SV-PA-017-2009) is one of most characteristic improved seeds in Xinjiang, is claimed Acker sago west by the Xinjiang local again, can make and do or eat raw.Fruit is oval, mean fruit weight 23.1g, fruit colourless look, no fine hair; The pulp yellow-white; Meat is thinner, and fiber is few, and juice is many, and it is sweet to distinguish the flavor of, and fragrance is arranged.Its soluble solid content 23%, quality is first-class; The pulp freestone, benevolence is sweet, and shell is thin, the heavy 1.78g of monokaryon, kernel percent 32.4%.Sweet crisp anti-the chewing of almond, oiliness, sugariness is long.
Most of apricot kinds are that selfing is not affine, and self-fruitful rate is low, and necessary reasonable disposition pollinated variety could obtain corresponding production and quality in the production.Also often because of the normal pollination underproduction of bad climate condition influence such as florescence low temperature, sand and dust and wet weather and reduction quality, artificial pollination is the important means that improves apricot output.
Artificial pollination is sent to plant pollen on the column cap to improve the technical measures of fruit-setting rate with manual method.On purpose select the parent to advance the necessary means of plant hybridization breeding.On production of fruit trees, to, monoecism shaky and different flower and dioecious fruit tree from spending; Under the situation that lacks pollinizers or florescence awful weather, the normal spontaneous pollination of influence, also often need carry out artificial pollination.On producing, use liquid always and dust, and add the material that promotes pollen germination and pollen tube growth in the solution of being everlasting, like boric acid, sucrose etc., to improve the pollination effect and to save the pollen consumption for the efficient that improves artificial pollination.
Optimal temperature and pH value are to guarantee pollen germination and pollen tube growth essential condition in the substratum, and too high or too low temperature and pH value all can suppress pollen germination and pollen tube growth.The result of study of Zhang Shaoling etc. shows that fruit plum blossom powder is sprouted and the required optimum pH of pollen tube growth is 6.5, and research shows that its appropriate pH value is 4.0～6.0 to Ding Changkui etc. to loquat pollen.Culture temperature also has material impact to pollen germination and pollen tube growth.
Promote the growth regulator of pollen germination and growth to mainly contain indolylacetic acid (IAA), naphthylacetic acid (NAA), Plant hormones regulators,gibberellins (GA
3), 2,4 dichlorophenoxyacetic acid (2,4-D), 6-benzyl aminoadenine (6-BA), indolebutyric acid (IBA) and alar-85 (B
9) etc.Except that short pollen germination of growth regulating class material and growth, certain chemical substance also has effect to pollen germination and growth.
Existing document record: Xing Jun, Yang Jie, Zheng Li. the Xinjiang apricot distributes and [J] studied in the feasibility analysis of preservation and freshness. Xinjiang University's journal (natural science edition), 2005 (2): 79-82.
Lu Zhicheng, Zhang Jingru, Wang Wei east. China Lee and apricot production status and development countermeasure [J]. agricultural technology service, 2003 (6): 4-6.
Du person of outstanding talent, Zhang Junmin. the gordian technique of apricot facility cultivation [J]. deciduous fruit tree, 2002 (5): 30～31.
Lu Chengzhi. Xinjiang apricot improved seeds are introduced [J]. Shanxi fruit tree, 2004 (11): 26～27.
Accord with green. urea and boron and growth regulator are to the influence [J] of lichee pollen germination and growth. Yunnan Normal University's journal (natural science edition), 2007,21 (3): 62～65
Zhang Shaoling, Mei Zhengmin, Chen Dixin. fruit plum in-vitro pollen germination and pollen tube growth influencing factors [J]. Chinese agronomy circular, 2003,19 (2): 21～25.
Ding Changkui, Chen Qifeng, Xia Qizhou. nutritive element and growth regulator are to the influence [J] of loquat pollen germination and setting. Chinese fruit tree, 1991 (4): 18～20,40.
Zheng Chengle, Yu Xiaoqu, Wang Xiaofei, etc. the research [J] in the life of chinquapin pollen. Agricultural University Of Jiangxi's journal, 2004,26 (2): 201～202.
Wang Shaoxian, Liu Baoguo, Li Yanmei, etc. the influence [J] of 6 kinds of vegetables pollen viabilities of short time high temperature. Luoyang agricultural higher junior college journal, 1999,19 (3): 1～2.
ADANIYAS.Optimal?pollination?environment?of?tetraploid?ginger(Zingiber?of?ficinale?Roscoe)evaluated?by?in?vitro?pollen?germination?and?pollen?tube?growth?in?styles[J].Scient?ia?Horticulture，2001，90：219～226.
The problem of existing pollen germination and growth existence: pollen germination rate is low, pollen tube growth is short, growth regulator is bought, use inconvenience etc., becomes the technical bottleneck that restriction this area is developed.
The present invention is directed to the low and slow dysgenesia of pollen tube growth of the white apricot pollen germination rate of Luntai, through research sucrose, boric acid, growth regulator, pH value and temperature obtain being fit to pollen germination and growth medium to the influence of white apricot pollen germination of Luntai and growth.
Summary of the invention
The objective of the invention is to, a kind of substratum that improves white apricot pollen germination of Luntai and growth is provided, this substratum is to be the minimum medium component by agar, sucrose and boric acid, and adding growth regulator more respectively is Plant hormones regulators,gibberellins (GA
3), indolylacetic acid (IAA), naphthylacetic acid (NAA), 2,4 dichlorophenoxyacetic acid (2,4-D) or 6-benzyl aminoadenine (6-BA) process, culture condition comprises temperature and pH value.It is low that this substratum has solved its pollen germination rate, and pollen tube is short and be difficult for the problem that pollination is fertilized, for the white apricot of Luntai finds a kind of effective way that improves germination rate.Improved white apricot pollen germination of Luntai and growth through substratum of the present invention, easy and simple to handle, cost is low, the cycle is short, germination rate is high.
A kind of substratum that improves white apricot pollen germination of Luntai and growth of the present invention, this substratum are to be 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 20-80mg/L Plant hormones regulators,gibberellins by mass ratio; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 1-5mg/L naphthylacetic acid; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 0.1-0.5mg/L 6-benzyl aminoadenine; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 1-5mg/L indolylacetic acid; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 20-40mg/L 2,4 dichlorophenoxyacetic acid are processed.
The preparation method of said substratum; With mass ratio is that 1% agar, 20-30% sucrose and 0.01-0.03% boric acid are the minimum medium component; Add growth regulator more respectively and be 20-80mg/L Plant hormones regulators,gibberellins, 1-5mg/L naphthylacetic acid, 0.1-0.5mg/L 6-benzyl aminoadenine, 1-5mg/L indolylacetic acid or 20-40mg/L 2,4 dichlorophenoxyacetic acid (2,4-D); Temperature is 20-30 ℃, and the pH value gets final product for 5.5-6.0 carries out mixed culture.
Technical scheme of the present invention: be on the prior art basis; Adopt in vitro culture method, the pollen that collection is good is being covered with on the slide glass of substratum with the uniform bullet of cotton rod; Substratum is the minimum medium component with the sucrose of 5-20%, boric acid and 1% agar of 0-0.1%; It is 10-30 ℃ that culture temperature is set, and the medium pH value is 4.0-8.0, adds the GA of 0-200mg/L more respectively
3, 0-40mg/L NAA, 0-40mg/L 6-BA, 0-80mg/L IAA and 0-60mg/L 2,4-D cultivates; Through germination rate (pollen germination number/pollen frequency) and pollen tube length index being carried out determination and analysis, the combination of screening optimal growth.It is low to have solved the white apricot pollen germination rate of Luntai, and pollen tube is the problem of weak point, finds the approach of effective raising pollen germination rate for the white apricot of Luntai.
A kind of substratum that improves white apricot pollen germination of Luntai and growth of the present invention, through implementing to reach following beneficial effect:
1, the present invention cultivates through selecting for use nutrient media components and culture condition to carry out pollen, promotes one of pollen germination and pollen tube growth effective way.
2, working method is simple, does not need the special technique means.
3, cost is low, the medium component low price, and the growth regulator that especially uses in the research has soluble in water, low price, buys advantages such as convenient, easy to use, when carrying out a large amount of liquid pollinations, can have more excellent net effect.
5, the invention solves the low and poky technical problem of the white apricot of Luntai germination rate on substratum, can be the white apricot artificial pollination of Luntai technical research and lay the foundation.
Nutrient media components has influence to pollen germination and growth, increases with sucrose concentration, and pollen germination rate and pollen growth are the trend of falling after rising; Increase with boric acid concentration, pollen germination rate and growth are the trend of falling after rising; With growth regulator GA3, NAA, IAA, 2,4-D or 6-BA concentration increase, and pollen germination rate and growth are the trend of falling after rising.
Culture temperature pollen germination and growth have the significance influence, and with the rising of temperature, pollen germination and growth are the trend of falling after rising, and temperature is suitable pollen germination and growth in the 20-30 ℃ of scope.
Medium pH value pollen germination and growth have the significance influence, and with the rising of pH, pollen germination and growth are the trend of falling after rising, and pH is suitable pollen germination and growth in the 5.5-6.0 scope.
Embodiment
The embodiment that the present invention is cited, but be not limited to described embodiment.
Embodiment
All reagent selected for use and instrument all are well known in the art.
The selection of pollen and processing:
Choose small bell phase bud, peel flower pesticide and contain in the sulfuric acid carton, place under the normal temperature and dry in the shade, treat the flower pesticide pollen that sheds that ftractures voluntarily, its collection is packed into seal in the centrifuge tube of 1.5ml is placed on the freezer compartment of refrigerator preservation;
Cultural method and time:
Substratum configuration: use 1% agar; Putting into concentration respectively is: 0,5%, 10%, 15%, 20% or 25% sucrose and concentration are respectively: 0,0.01%, 0.03%, 0.05% or 0.1% boric acid is handled; The substratum of different concns is upside down on the slide glass; With cotton rod with the uniform bullet of pollen on the substratum of slide glass, be placed on the bottom and be covered with in the petridish of moistening gauze, every gradient repeats 3 times; Place 25 ℃ of thermostat containers of temperature to cultivate, observe the length of germination rate and pollen tube behind the 24h;
Filter out pollen granule number/pollen granule sum * 100% of optimum medium germination rate=sprouted.
Temperature is provided with the substratum temperature and is respectively 10,15,20,25 and 30 ℃ and cultivates under the optimum medium component, takes out substratum behind the 4h and measures pollen germination rate and pollen tube length, confirms the righttest culture temperature;
The pH value is under the optimum medium component; Use 1mol/L hydrochloric acid and 1mol/L sodium hydroxide solution to regulate the medium pH value; The pH value is respectively 4.0,4.5,5.0,5.5,6.0,6.5,7.0,7.5 and 8.0; Take out mensuration pollen germination rate and pollen tube length after cultivating 4h, confirm the optimum pH of substratum.
The preparation mass ratio is that 1% agar, 25% sucrose and 0.01% boric acid are the minimum medium component, adds Plant hormones regulators,gibberellins (GA more respectively
3), indolylacetic acid (IAA), naphthylacetic acid (NAA), 2,4 dichlorophenoxyacetic acid (2,4-D) or 6-benzyl aminoadenine (6-BA) mix, wherein 5 kinds of growth regulator concentration gradients are:
Prepared culture medium is inverted on the slide glass sheet; Place the substratum of the different growth regulators of different concns to cultivate with gathering good pollen; Carry out constant-temperature moisture-keeping immediately and cultivate, cultivate 4h and observe pollen germination and sprout length, observe 5 visuals field for every; Through pollen germination rate and pollen tube length index are carried out determination and analysis, select the optimal growth component.
Different nutrient media componentses and growth conditions are to the influence of white apricot pollen germination of Luntai and growth:
Place different substratum to cultivate with gathering good pollen,, select the optimal growth combination and see table 1,2,3 and 4 through pollen germination rate and pollen tube length index are carried out determination and analysis.
The different nutrient media componentses of table 1 are to the influence of apricot pollen germination rate and pollen tube growth:
The different culture temperature of table 2 are to the influence of apricot pollen germination rate and pollen tube growth
The different pH values of table 3 are to the influence of apricot pollen germination rate and pollen tube growth
The different growth regulators of table 4 are to the influence of apricot pollen germination rate and pollen tube growth
Annotate: the new multipole difference of Deng Kenshi compares, and the mark same letter is represented indifference in the same row, different lowercase alphabet differentials different significantly (p＜0.05), and different capitalizations are represented difference extremely significantly (p＜0.01).
Conclusion: can be found out with 4 by table 1,2,3: different nutrient media componentses have very big influence to white apricot pollen germination of Luntai and growth; Sucrose and boric acid all present high density and suppress the promoted effect of lower concentration; Pollen germination and growth are adapted at carrying out under little acid environment, and high temperature or low temperature all are unfavorable for pollen germination and growth.Growth regulator influences size to pollen germination: GA
3＜IAA＜NAA＜2,4-D＜6-BA.
Carrying out pollen germination and growth through nutrient media components of the present invention and growth regulator and concentration thereof, is to improve the white apricot pollen germination of Luntai and one of growth effective way.
Claims (2)
1. a substratum that improves white apricot pollen germination of Luntai and growth is characterized in that, this substratum is to be the Plant hormones regulators,gibberellins of 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 20-80mg/L by mass ratio; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 1-5mg/L naphthylacetic acid; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 0.1-0.5mg/L 6-benzyl aminoadenine; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 1-5mg/L indolylacetic acid; Or 1% agar, 20-30% sucrose, 0.01-0.03% boric acid and 20-40mg/L 2,4 dichlorophenoxyacetic acid are processed.
2. the preparation method of substratum according to claim 1; It is characterized in that with mass ratio being that 1% agar, 20-30% sucrose and 0.01-0.03% boric acid are the minimum medium component; Adding growth regulator more respectively is 20-80mg/L Plant hormones regulators,gibberellins, 1-5mg/L naphthylacetic acid, 0.1-0.5mg/L 6-benzyl aminoadenine, 1-5mg/L indolylacetic acid or 20-40mg/L 2; 4-dichlorphenoxyacetic acid, temperature are 20-30 ℃, and the pH value gets final product for 5.5-6.0 carries out mixed culture.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618485A (en) * | 2012-04-01 | 2012-08-01 | 新疆农业大学 | Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen |
| CN103421737A (en) * | 2013-06-04 | 2013-12-04 | 塔里木大学 | Culture medium for promoting germination of Amygdalus L. pollen, culture method and application thereof |
| CN104004702A (en) * | 2014-05-20 | 2014-08-27 | 四川农业大学 | Culture medium for germination of tetraploid hemarthria compressa pollen |
| CN105237153A (en) * | 2015-09-06 | 2016-01-13 | 广西壮族自治区药用植物园 | Fruit retention agent of momordica grosvenori, preparation method and application thereof |
| CN115005094A (en) * | 2022-06-29 | 2022-09-06 | 贵州师范大学 | Composition for improving artificial hybridization fertilization success rate of polygonatum sibiricum, and improving method and application thereof |
| CN115812507A (en) * | 2022-12-12 | 2023-03-21 | 新疆农业科学院园艺作物研究所 | Method for improving fruit setting rate and/or yield of Muya-Ge apricots |
-
2011
- 2011-12-15 CN CN2011104204470A patent/CN102517249A/en active Pending
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| 刘会超: "离体萌发法测定垂丝海棠花粉生活力的研究", 《山西农业科学》 * |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618485A (en) * | 2012-04-01 | 2012-08-01 | 新疆农业大学 | Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen |
| CN103421737A (en) * | 2013-06-04 | 2013-12-04 | 塔里木大学 | Culture medium for promoting germination of Amygdalus L. pollen, culture method and application thereof |
| CN103421737B (en) * | 2013-06-04 | 2015-04-15 | 塔里木大学 | Culture medium for promoting germination of Amygdalus L. pollen, culture method and application thereof |
| CN104004702A (en) * | 2014-05-20 | 2014-08-27 | 四川农业大学 | Culture medium for germination of tetraploid hemarthria compressa pollen |
| CN105237153A (en) * | 2015-09-06 | 2016-01-13 | 广西壮族自治区药用植物园 | Fruit retention agent of momordica grosvenori, preparation method and application thereof |
| CN115005094A (en) * | 2022-06-29 | 2022-09-06 | 贵州师范大学 | Composition for improving artificial hybridization fertilization success rate of polygonatum sibiricum, and improving method and application thereof |
| CN115812507A (en) * | 2022-12-12 | 2023-03-21 | 新疆农业科学院园艺作物研究所 | Method for improving fruit setting rate and/or yield of Muya-Ge apricots |
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Application publication date: 20120627 |