CN102512442B - Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same - Google Patents
Zinc chelate-containing compound vitamin oral nano-emulsion and preparation method for same Download PDFInfo
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- CN102512442B CN102512442B CN 201110421123 CN201110421123A CN102512442B CN 102512442 B CN102512442 B CN 102512442B CN 201110421123 CN201110421123 CN 201110421123 CN 201110421123 A CN201110421123 A CN 201110421123A CN 102512442 B CN102512442 B CN 102512442B
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Abstract
The invention relates to a veterinary compound oral nano-emulsion containing zinc chelate, selenium and multivitamins and a preparation method for the same, wherein the veterinary compound oral nano-emulsion comprises the following components: zinc gluconate, a chelant, vitamin A, vitamin D3, vitamin E, sodium selenite, an emulsifier, an emulsifying aid, a preservative, an antioxidant and water. The veterinary compound oral nano-emulsion disclosed by the invention can comprehensively supplement vitamins; moreover, zinc can enhance esterase activity and thus promoting absorption for vitamin A, vitamin D can promote skeletal development, and selenium can promote absorption for vitamin E and enhance the antioxidation of vitamin E; and when toxic symptom appears due to excessive selenium, vitamin E can enhance excretion for selenium and decrease toxicity.
Description
Technical field
The present invention relates to a kind of chelated zinc, selenium and multivitamin animal compound oral administration nanometer emulsion formulation and preparation method thereof of containing, belong to the veterinary drug preparation technical field.
Background technology
Zinc is growth of animals or poultry, breed necessary trace element.When the zinc in zinc deficiency in the feedstuff or the feedstuff was difficult for being absorbed by poultry, zinc deficiency will appear in poultry, causes the human body of poultry to be become thin, and crust thickens, the obvious atrophy of the testis of breeding stock, stud bird, and oligospermia is grown, is bred all and be affected.Therefore, suitably zinc supplement in the livestock and poultry cultivation process.
The product of at present zinc supplement mostly is inorganic zinc, and such as zinc sulfate, zinc carbonate, zinc oxide etc., the zinc bioavailability of these inorganic states is lower.Therefore, along with improving constantly of preparation level, chelated zinc has replaced the inorganic zinc of difficult absorption gradually, it is the chelate that a class has unique circulus, integrate aminoacid and zinc element, be to have form and the similar zinc element additive of biological function in a kind of and the animal body, main Types has zinc lysine, zinc methionine.Zinc-amino acid chelate has following two large advantages: (1) steady chemical structure is difficult for being combined into insoluble compound with other materials or being attracted on the insoluble colloid.(2) biological value is high, not only absorbs soon, and can reduce biochemical process, saves energy i (in vivo) consumption.
Chinese patent file CN1467199A (application number 02136013.8) provides a kind of L-Lysine Zn of Chelate made from biochemical method, it can replenish the necessary metallic element zinc of organism and lysine, this material is used for control owing to zinc deficiency lacks the various diseases that lysine causes, and can make the body recovery that is in sub-health state to health status.
Zinc and other trace element such as vitamin, selenium etc. are all growth of animals or poultry, breed requisite trace element; At present, vitamin and selenium and other trace elements often adopt the nano-emulsion technology to prepare oral formulations.The nano-emulsion technology is preparation technique emerging in this area, and it is that particle diameter is Thermodynamically stable, the isotropism of 1～100nm, transparent or semitransparent homodisperse system by spontaneous formation such as water, oil, surfactant and cosurfactants.
Therefore, the required zinc of poultry, selenium and various vitamin are prepared into nano-emulsion, but the assimilation effect of each trace element of Effective Raise.But the product that contains chelated zinc and vitamin, selenium and other trace elements that adopts that existing nano-emulsion technology makes exist separate out, the shortcoming such as precipitation, affected the product effect of bringing into normal play.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, a kind of chelated zinc, selenium, vitamin A, vitamin D of containing simultaneously is provided
3, vitamin E oral administration nanometer emulsion formulation and preparation method thereof.
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 50～90g, chelating agen 45～90g, vitamin A 4～14g, vitamin D
30.3～0.9g, vitamin e1 5～30g, sodium selenite 0.1～0.5g, emulsifying agent 150～350g, co-emulsifier 80～220g, antiseptic 0.1～2.0g, antioxidant 0.1～2.0g, water is settled to 1L.
Preferably, every liter of component is as follows:
Zinc gluconate 75～80g, chelating agen 60～85g, vitamin A 5～10g, vitamin D
30.4～0.7g, vitamin E 15～25g, sodium selenite 0.2～0.4g, emulsifying agent 200～300g, co-emulsifier 90～150g, antiseptic 0.2～0.5g, antioxidant 0.2～0.3g, water is settled to 1L.
Described chelating agen is a kind of or combination in lysine hydrochloride, lysine alkali, methionine, glycine, the arginine; Preferred lysine hydrochloride or methionine; Further preferred lysine hydrochloride.
Described emulsifying agent is tween 80, Arlacel-80, a kind of or combination among polyoxyethylene hydrogenated Oleum Ricini RH-40, the polyoxyethylene castor oil EL-35, preferred tween 80 or polyoxyethylene castor oil EL-35.
Described co-emulsifier is a kind of or combination in isopropyl alcohol, propylene glycol, Polyethylene Glycol, the glycerol, preferred Polyethylene Glycol or isopropyl alcohol.
Described antiseptic is selected from ethyl hydroxybenzoate, methyl hydroxybenzoate or benzoic acid, preferred ethyl hydroxybenzoate;
Described antioxidant is 2,6-di-tert-butyl-4-methy phenol (BHT), Butylated hydroxyanisole (BHA), sodium thiosulfate; Preferred 2, the 6-di-tert-butyl-4-methy phenol.
The above-mentioned preparation method that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc comprises the steps:
(1) take by weighing in proportion zinc gluconate, chelating agen, add in the suitable quantity of water, 20～40 ℃ of stirring and dissolving add sodium selenite again, after the solution clarification, through membrane filtration, get chelated zinc solution;
(2) in proportion weighing vitamin A, vitamin D
3, vitamin E, add antiseptic, antioxidant, be stirred to add emulsifying agent after the fully dissolving, co-emulsifier stirs to clarification, temperature transfers to 20～40 ℃, slowly adds the zinc solution that closes that step (1) makes, water is settled to 1L, and get final product.
Filter membrane in the described step (1) is 5 μ m filter membranes.
Vitamin A described in the present invention, vitamin D
3Being the vitamin that meets feedstuff national standard purity with vitamin E, is oily under the room temperature.
Beneficial effect
1, the comprehensive vitimin supplement of the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc of the present invention, and zinc can strengthen esterase active and promote the absorption of vitamin A, vitamin D can promote skeleton development, and selenium can promote the absorption of vitamin E, strengthens its antioxidation; When selenium is excessive when poisoning symptom occurring, vitamin E can increase the drainage of selenium, reduces toxicity; The compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc of the present invention is compared existing like product improvement amplitude and is reached more than 6%, sees the contrast test among the embodiment for details.
2, preparation method of the present invention adopts unique chelated zinc preparation technology, the chelated zinc that the method makes can stable existence in nano-emulsion preparation, chelated zinc and nano-emulsion can dual promotion zinc absorption, simultaneously, the chelated zinc that the chelated zinc that adopts this method to make is produced than traditional handicraft is compared and is not separated out, do not precipitate, and sees the contrast test among the embodiment for details.
3, to have technique simple for preparation method of the present invention, is suitable for large-scale industrial production.
Description of drawings
Fig. 1 is that the product of embodiment 1 preparation is observed photo at the sample behind the 180d that keeps sample under 0 ℃, 25 ℃, 40 ℃ respectively;
Wherein: the left side be the sample of 180d of keeping sample under 0 ℃, middle is keep sample under the 25 ℃ sample of 180d, the right side be the sample of 180d of keeping sample under 40 ℃.
The specific embodiment
The present invention will be further described below in conjunction with embodiment, but institute of the present invention protection domain is not limited to this.Polyoxyethylene castor oil EL-35 asks commerce and trade Co., Ltd available from Beijing phoenix gift essence; 2,6-di-tert-butyl-4-methy phenol (BHT) is available from Chemical Reagent Co., Ltd., Sinopharm Group; Other raw materials are commercially available prod commonly used.
Embodiment 1
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 75g, lysine hydrochloride 60g, vitamin A 5g, vitamin D
30.7g, vitamin E 20g, sodium selenite 0.2g, tween 80 250g, propylene glycol 120g, ethyl hydroxybenzoate 0.2g, 2,6-di-tert-butyl-4-methy phenol (BHT) 0.2g, water is settled to 1L.
The above-mentioned preparation method that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc comprises the steps:
(1) take by weighing in proportion zinc gluconate, lysine hydrochloride in water, 40 ℃ of abundant stirring and dissolving add sodium selenite again, after the solution clarification, cross 5 μ m filter membranes, and are for subsequent use;
(2) take by weighing in proportion VA oil, VD3 oil, VE is oily pours in the dry retort, add antiseptic, antioxidant, being stirred to fully, the rear adding of dissolving emulsifying agent, co-emulsifier stir to clarification, temperature transfers to 40 ℃, slowly add step (1) and make chelated zinc solution, water is settled to 1L, and get final product.
Zinc dissolubility contrast test
, the prepared chelated zinc of patent 02136013.8 prepares nano-emulsion according to the condition of step (2) in this patent embodiment 1 preparation method use respectively the identical inorganic zinc of zinc content, commercially available chelated zinc (manufacturer: Chengdu Shi Punuo Bioisystech Co., Ltd), get simultaneously the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc that the identical embodiment of zinc content 1 makes, the product physical behavior of contrast, the result is as shown in table 1:
Table 1
The result shows: this product granule is near hydrone, and is extremely strong with the bonding force of water, can produce high dissolving diffusibility; Can unrestrictedly mix with water.The product of conventional zinc and commercially available chelated zinc uses by drinking-water, and easy precipitation after the dissolving is stopped up water fountain, and the present invention be dissolved in water rear as clear as crystal evenly, without precipitation, can not stop up water fountain.
The reserved sample observing test
Get part embodiment 1 prepared nano-emulsion, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence, as shown in Figure 1.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 39nm.
The product weight increment test
(1) EXPERIMENTAL DESIGN
The pig of a pig house is divided into three groups, be respectively matched group, 1 group of test and test 2 groups, every group of 8 pigs, wherein matched group does not add any vitamin products, test 1 group of electrolysis multidimensional product (manufacturer: the great Bioisystech Co., Ltd that reaches of Shijiazhuang City) that in drinking-water, adds commercially available sulfur acid zinc, consumption is tested 2 groups and add the compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc that 500ml embodiment 1 makes in every 1000kg drinking-water by recommending consumption.Contrast test has been carried out three batches altogether, and is as shown in table 1.
Table 2
(2) experimental animal
Search for food normally the 12-23kg children pig that body weight is consistent after selecting wean.Every batch of test pig is divided into three groups by age in days and body weight.On-test,, the group average weight of every batch of pig was consistent, and end is tested when body weight reaches 65-75kg.
The test natural law is 45 days, and accurate recording is respectively organized feed intake, each settlement of term end business accounts honest material amount.The test pig individuality is weighed simultaneously, calculates each feed consumption of organizing/weightening finish ratio.
Experimental result
9 groups of observed datas of three batches of tests of arrangement statistics, row are such as table 3, table 4 and table 5.Because the symmetrical grouping of each batch test chamber, and each batch of beginning body weight is different, so when significance test of difference, adopt in batches paired t-test method between group.
Table 3
* the P value is paired t-test measurement result between each batch organized by stages
Conclusion: by as seen from Table 3, every daily feed intake of test pig is tested 2 groups and is tested 1 group and on average reduced by 1.4% between 1.75-1.85kg, has on average reduced by 2% than matched group, and group difference is not remarkable.
The average daily gain measurement result sees Table 4.
Table 4
* the P value is paired t-test measurement result between each batch group
Conclusion: by as seen from Table 4, test 2 groups and be significantly higher than matched group.
According to computing formula:
Feed conversion rate=feed consumption/weightening finish
Result of calculation shows that the nano-emulsion that trace element contains zinc-amino acid chelate demonstrates obvious superiority.Testing 2 groups of average feed conversion rates is 2.74, and testing 1 group of average feed conversion rate is 2.94.
Calculate the improvement amplitude according to following formula:
As calculated, the improvement amplitude of testing 2 groups (products that the present invention makes) reaches 6.8%, difference highly significant (P=0.004), and the result is as shown in table 5.
Table 5
* the P value is paired t-test measurement result between each batch group
Conclusion: through the test can find out, the compound vitamin nano-emulsion of zinc-amino acid chelate aspect daily gain, feedstuff-meat ratio significantly better than the commercially available prod.
Embodiment 2
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 76g, methionine 55g, vitamin A 6g, vitamin D
30.6g, vitamin E 15g, sodium selenite 0.3g, tween 80 280g, Polyethylene Glycol 100g, ethyl hydroxybenzoate 0.2g, BHT 0.2g, water is settled to 1L.
Preparation method is with embodiment 1.
The reserved sample observing test
Get part embodiment 2 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above five kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 41nm.
The product weight increment test
Experimental procedure is with embodiment 1, and after testing, the improvement amplitude reaches 7.1%.
Embodiment 3
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 78g, methionine 68g, vitamin A 10g, vitamin D
30.5g, vitamin E 23g, sodium selenite 0.2g, polyoxyethylene castor oil EL-35 300g, Polyethylene Glycol 150g, ethyl hydroxybenzoate 0.4g, BHT 0.2g, water is settled to 1L.
Preparation method is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 43nm.
The product weight increment test
Experimental procedure is with embodiment 1, and after testing, the improvement amplitude reaches 7.4%.
Embodiment 4
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 78g, lysine hydrochloride 75g, vitamin A 5g, vitamin D
30.6g, vitamin E 16g, sodium selenite 0.3g, polyoxyethylene castor oil EL-35 220g, isopropyl alcohol 150g, ethyl hydroxybenzoate 0.5g, BHT 0.3g, water is settled to 1L.
Preparation method is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 47nm.
The product weight increment test
Experimental procedure is with embodiment 1, and after testing, the improvement amplitude reaches 7.2%.
Embodiment 5
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 76g, lysine hydrochloride 80g, vitamin A 10g, vitamin D
30.7g, vitamin E 25g, sodium selenite 0.4g, tween 80 300g, isopropyl alcohol 100g, ethyl hydroxybenzoate 0.5g, BHT 0.3g, water is settled to 1L.
Preparation method is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 49nm.
The product weight increment test
Experimental procedure is with embodiment 1, and after testing, the improvement amplitude reaches 7.4%.
Embodiment 6
A kind of compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc, every liter of component is as follows:
Zinc gluconate 80g, lysine hydrochloride 85g, vitamin A 5g, vitamin D
30.4g, vitamin E 15g, sodium selenite 0.2g, tween 80 220g, isopropyl alcohol 100g, ethyl hydroxybenzoate 0.2g, BHT 0.2g, water is settled to 1L.
Preparation method is with embodiment 1.
The reserved sample observing test
Get part embodiment 3 prepared nano-emulsions, be sub-packed in several vials, placing respectively after the sealing keeps sample under 0 ℃, 25 ℃, the 40 ℃ conditions investigates 180d, observes every the 10d sampling.The result shows that this nano-emulsion all keeps limpid, transparent outward appearance under above three kinds of temperature conditions, does not occur separating out, the phenomenon such as precipitation, opalescence.Observe under the transmission electron microscope, the drop of this nano-emulsion is spherical in shape, and is evenly distributed, favorable dispersibility, and measuring mean diameter with the particle diameter tester is 46nm.
The product weight increment test
Experimental procedure is with embodiment 1, and after testing, the improvement amplitude reaches 7.5%.
Claims (2)
1. compound vitamin oral administration nanometer emulsion formulation that contains chelated zinc is characterized in that every liter of component is as follows:
Zinc gluconate 50 ~ 90g, chelating agen 45 ~ 90g, vitamin A 4 ~ 14g, vitamin D
30.3 ~ 0.9g, vitamin E 15 ~ 30g, sodium selenite 0.1 ~ 0.5g, emulsifying agent 150 ~ 350g, co-emulsifier 80 ~ 220g, antiseptic 0.1 ~ 2.0g, antioxidant 0.1 ~ 2.0g, water is settled to 1L;
As follows preparation:
(1) take by weighing in proportion zinc gluconate, chelating agen, add in the suitable quantity of water, 20 ~ 40 ℃ of stirring and dissolving add sodium selenite again, after the solution clarification, through membrane filtration, get chelated zinc solution;
(2) in proportion weighing vitamin A, vitamin D
3, vitamin E, add antiseptic, antioxidant, be stirred to add emulsifying agent after the fully dissolving, co-emulsifier stirs to clarification, temperature transfers to 20 ~ 40 ℃, slowly adds the chelated zinc solution that step (1) makes, water is settled to 1L, and get final product;
Described chelating agen is a kind of or combination in lysine hydrochloride, lysine alkali, methionine, glycine, the arginine.
2. compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that every liter of component is as follows:
Zinc gluconate 75 ~ 80g, chelating agen 60 ~ 85g, vitamin A 5 ~ 10g, vitamin D
30.4 ~ 0.7g, vitamin E 15 ~ 25g, sodium selenite 0.2 ~ 0.4g, emulsifying agent 200 ~ 300g, co-emulsifier 90 ~ 150g, antiseptic 0.2 ~ 0.5g, antioxidant 0.2 ~ 0.3g, water is settled to 1L.
3, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, described chelating agen is lysine hydrochloride or methionine.
4, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 3 is characterized in that, described chelating agen is lysine hydrochloride.
5, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, described emulsifying agent is tween 80, Arlacel-80, a kind of or combination among polyoxyethylene hydrogenated Oleum Ricini RH-40, the polyoxyethylene castor oil EL-35.
6, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 5 is characterized in that, described emulsifying agent is tween 80 or polyoxyethylene castor oil EL-35.
7, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, described co-emulsifier is a kind of or combination in isopropyl alcohol, propylene glycol, Polyethylene Glycol, the glycerol.
8, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 7 is characterized in that, described co-emulsifier is Polyethylene Glycol or isopropyl alcohol.
9, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that described antiseptic is selected from ethyl hydroxybenzoate, methyl hydroxybenzoate or benzoic acid.
10, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 9 is characterized in that, described antiseptic is ethyl hydroxybenzoate.
11, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 1 is characterized in that, described antioxidant is 2,6-di-tert-butyl-4-methy phenol, Butylated hydroxyanisole, sodium thiosulfate.
12, compound vitamin oral administration nanometer emulsion formulation as claimed in claim 11 is characterized in that, described antioxidant is 2,6-di-tert-butyl-4-methy phenol.
13, the described preparation method that contains the compound vitamin oral administration nanometer emulsion formulation of chelated zinc of claim 1 is characterized in that, comprises the steps:
(1) take by weighing in proportion zinc gluconate, chelating agen, add in the suitable quantity of water, 20 ~ 40 ℃ of stirring and dissolving add sodium selenite again, after the solution clarification, through membrane filtration, get chelated zinc solution;
(2) in proportion weighing vitamin A, vitamin D
3, vitamin E, add antiseptic, antioxidant, be stirred to add emulsifying agent after the fully dissolving, co-emulsifier stirs to clarification, temperature transfers to 20 ~ 40 ℃, slowly adds the chelated zinc solution that step (1) makes, water is settled to 1L, and get final product.
14, preparation method as claimed in claim 13 is characterized in that, the filter membrane in the described step (2) is 5 μ m filter membranes.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520102A (en) * | 2013-10-14 | 2014-01-22 | 陕西科技大学 | Vitamin D nano-emulsion and preparation method thereof |
| CN105053561A (en) * | 2015-07-29 | 2015-11-18 | 山东迅达康生物科技有限公司 | Nano-level feed additive and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1467199A (en) * | 2002-07-12 | 2004-01-14 | 上海福赐德营养保健品有限责任公司 | Monozinc di-L-lysine |
| CN101982176B (en) * | 2010-10-18 | 2012-07-04 | 齐鲁动物保健品有限公司 | Compound sodium selenite-vitamin E oral nano-emulsion preparation for livestock and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103520102A (en) * | 2013-10-14 | 2014-01-22 | 陕西科技大学 | Vitamin D nano-emulsion and preparation method thereof |
| CN105053561A (en) * | 2015-07-29 | 2015-11-18 | 山东迅达康生物科技有限公司 | Nano-level feed additive and preparation method thereof |
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