CN102511708A - Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof - Google Patents
Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof Download PDFInfo
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- CN102511708A CN102511708A CN2011104520656A CN201110452065A CN102511708A CN 102511708 A CN102511708 A CN 102511708A CN 2011104520656 A CN2011104520656 A CN 2011104520656A CN 201110452065 A CN201110452065 A CN 201110452065A CN 102511708 A CN102511708 A CN 102511708A
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Abstract
The invention relates to glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof. The glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules are mainly made of, by weight, 8%-12% of glycyrrhiza polysaccharide, 2%-6% of honeysuckle extract, 2%-6% of Chinese date extract, 1%-3% of honey, and 73%-87% of accessories. The health granules mainly made of the glycyrrhiza polysaccharide with the honeysuckle, the Chinese date, the honey and the like bridge the gap of the glycyrrhiza polysaccharide in terms of health product development, and have the effects of immunity improvement and fatigue resistance. The method extracting glycyrrhizae suspension cells to obtain the glycyrrhiza polysaccharide is insusceptible to seasons and regions and is convenient and easy in operation.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof, particularly a kind of Chinese medicinal granule health food.
Background technology
Radix Glycyrrhizae is as a kind of traditional Chinese medicine; The good reputation of " ten sides, nine grass " is just arranged since ancient times, and the main active of Radix Glycyrrhizae has glycyrrhizic acid, Radix Glycyrrhizae brass and licorice polysaccharide, and the above two have had more deep research; And that the functional study of licorice polysaccharide mainly concentrates on is antitumor; Antiviral, regulate immunocompetence and remove the free radical aspect, seldom in the exploitation of licorice polysaccharide application facet.The Radix Glycyrrhizae suspension cell has more development potentiality as a kind of new approach of licorice polysaccharide of producing, and its licorice polysaccharide content of producing is higher than wild Radix Glycyrrhizae, has significantly anti-oxidant, antiviral, antitumor, immunoregulatory activity; Honeysuckle is caprifoliaceae plant (Lonicera japonica Thunb). the flower that the dry flower of honeysuckle or band are just opened.It is a kind of clinical conventional Chinese medicine, have clearing heat and detoxicating, the effect of wind-heat dissipating.Date traditional Chinese medicine theory is thought; Date has effects such as qi-restoratives benefit gas, nourishing blood and tranquilization, strengthening the spleen and stomach; Be patient's good health care nutriment such as weakness of the spleen and the stomach, insufficiency of vital energy and blood, lassitude, insomnia and dreamful sleep, date is the health food of integration of drinking and medicinal herbs, has very high nutritive value.Honey has a great health care function as a kind of, can not only liver protecting, can regulate nervous system simultaneously, and neurasthenia, insomnia etc. is had a significant effect.At the health products development field, use kind or blank out that the licorice polysaccharide preparation improves immunity.
Summary of the invention
In order to solve the problems of the prior art, the invention provides a kind of licorice polysaccharide honeysuckle date honey particles agent and preparation method thereof, solved the problem of licorice polysaccharide in health products research and development field blank.
The present invention realizes through following technical scheme:
The agent of a kind of licorice polysaccharide honeysuckle date honey particles, primary raw material is to be made up of the honeysuckle of the licorice polysaccharide of method for tissue culture production and integration of drinking and medicinal herbs, date and honey, is aided with other material, wherein primary raw material mass ratio therein is following:
Described carbohydrate auxiliary material is selected from a kind of of lactose, dextrin, sucrose, xylitol or sweet mellow wine, or their mixture.
The preparation method of a kind of licorice polysaccharide honeysuckle date honey particles agent comprises the steps:
(1) extraction of licorice polysaccharide: the extracting liquorice cell powder, the distilled water of 15-25 times of weight of adding leaves standstill 5-12h, boiling 1-5 time, each 1-3h.Repeat boiling 2-5 time, merging filtrate is concentrated to certain volume, is 50-90% with absolute ethyl alcohol modulation final concentration, and 0-6 ℃ of hold over night removed supernatant, with sevage method deproteinized, obtains the licorice polysaccharide powder;
(2) preparation of honeysuckle concrete: honeysuckle water is extracted 1～3 time, and each amount of water is 10～20 times of medicinal material weight, and extraction time is each 1～3h, and extracting temperature is 50～90 ℃, and the extract concentrate drying is become medicinal extract;
(3) preparation of Chinese date extract: with Removing Kernel for Date, oven dry is pulverized, and adds 10～15 times of water by volume, refluxing extraction 2～3 times, and extraction time is each 1～3h, and extracting temperature is 80～95 ℃, and the merging extract carries out low-press thick and shortens medicinal extract into;
(4) with above step extract and honey as major ingredient, add major ingredient and auxiliary material according to aforementioned proportion, mix, granulate, drying, whole grain promptly obtains licorice polysaccharide honeysuckle date honey granule.
Said licorice polysaccharide is to obtain by extracting in the Radix Glycyrrhizae suspension cell, and its preparation method is:
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 5%～15% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 200r/min～500r/min, and temperature is 20 ℃～30 ℃, sterilization time 20min～40min; MS contains 2%-5% sucrose in cultivating, and pH 5～6 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 20～28 ℃ of illumination boxs, cultivated 30～35 days, obtain the Radix Glycyrrhizae aseptic seedling.
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable.The callus subculture medium is the MS culture medium, adds 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH 5～6 before the sterilization, subculture cycle 25～30 days.
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium.Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, cultivate 3～5 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 15～20 days subcultures once.Shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃.Through the screening of going down to posterity for several times, the excellent clone of final obtained performance.
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor (number of patent application 201110255311.9) of design voluntarily; Inoculum concentration is 5%～10% fresh weight licorice cell; The fermentation tank throughput is 200L/min～500L/min; Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH value are 5～9, cultivation cycle is 15～20 days.
Advantage of the present invention is to fill up the blank of licorice polysaccharide aspect the health products exploitation; Proposing a kind of is major ingredient with the licorice polysaccharide; Joining gold honeysuckle flower, date, honey and other substances are developed a kind of health granule of alleviating insomnia, improving immunity, anti-fatigue effect that has simultaneously.Utilize the Radix Glycyrrhizae suspension cell to extract and obtain licorice polysaccharide, this method does not receive the restriction of season and region, and is convenient, easy to operate.
The specific embodiment
Below in conjunction with specific embodiment the present invention is further described.
The preparation of licorice polysaccharide
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 10% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 300r/min, and temperature is 25 ℃, sterilization time 30min; MS contains 3% sucrose in cultivating, and pH 5.9 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 25 ℃ of illumination boxs, cultivated 30 days, obtain the Radix Glycyrrhizae aseptic seedling.
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable.The callus subculture medium is the MS culture medium, adds 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, pH 5.9 before the sterilization, subculture cycle 28 days.
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium.Cultivation and composition are the 3/4MS culture medium, add 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, cultivate 3 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 18 days subcultures once.Shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃.Through the screening of going down to posterity for several times, the excellent clone of final obtained performance.
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor of design voluntarily, inoculum concentration is 8% fresh weight licorice cell, and the fermentation tank throughput is 300L//min, and cultivation and composition are the 3/4MS culture medium, adds 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, pH value are 5.9, cultivation cycle is 18 days.
Embodiment 1:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 20 times of weight, leave standstill 12h; 1h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 3 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 3 times, and each amount of water is 10 times of medicinal material weight, and extraction time is each 1h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 15 times of raw medicinal herbs, and extraction time is each 1.5h, and extracting temperature is 95 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g lactose; 100g sucrose after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, cross 20 eye mesh screens and prepare particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 2:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 15 times of weight, leave standstill 8h; 2h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 2 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 1 time, and each amount of water is 15 times of medicinal material weight, and extraction time is each 3h, and extracting temperature is 90 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 2 times, and each amount of water is 10 times of raw medicinal herbs, and extraction time is each 2.5h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g ethyl cellulose; The 100g xylitol after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 3:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 25 times of weight, leave standstill 5h; 3h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 2 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 2 times, and each amount of water is 20 times of medicinal material weight, and extraction time is each 2h, and extracting temperature is 50 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 12 times of raw medicinal herbs, and extraction time is each 1h, and extracting temperature is 90 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g dextrin; 100g sweet mellow wine after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 4:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 20 times of weight, leave standstill 12h; 1h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 3 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 3 times, and each amount of water is 10 times of medicinal material weight, and extraction time is each 1h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 15 times of raw medicinal herbs, and extraction time is each 1.5h, and extracting temperature is 95 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g lactose; 50g sweet mellow wine and 50g xylitol after mixing, are crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Test Example:
Undertaken by the function of enhancing immunity method of inspection in " the health food function assessment assessment process and the method for inspection standard " of Ministry of Public Health's issue.Tried the long-pending 20mL/kg of being of filling body of stomach of thing, every day 1 time, 30d continuously.Adopt the SAS statistical analysis software that data are carried out the average that variance analysis adds the ConA hole with Dunnett ' st check mtt assay (the mouse spleen lymphocyte conversion test that ConA induces) to each group and do not add ConA hole absorbance difference and carry out one-way analysis of variance; Group difference is (F=3.38 significantly; P＜0.05) through Dunnett ' st check, the granular preparation high dose group adds the ConA hole and sees table 1 with the difference average that does not add ConA hole absorbance and remarkable (P＜0.05) result of negative control group comparing difference.
The influence (X SD) of the mouse spleen lymphocyte conversion test that table 1 granular preparation is induced ConA
The SPL conversion test of ConA inducing mouse is the result show, granular preparation has the effect that strengthens the mouse cell immunologic function.
Claims (4)
1. licorice polysaccharide honeysuckle date honey particles agent; It is characterized in that; Primary raw material is to be made up of the honeysuckle of the licorice polysaccharide of method for tissue culture production and integration of drinking and medicinal herbs, date and honey, is aided with other material, and wherein primary raw material mass ratio therein is following:
2. according to the agent of the said licorice polysaccharide honeysuckle of claim 1 date honey particles, it is characterized in that described auxiliary material is selected from a kind of of lactose, dextrin, sucrose, xylitol or sweet mellow wine, or their mixture.
3. the preparation method of licorice polysaccharide honeysuckle date honey particles agent is characterized in that, comprises the steps:
(1) extraction of licorice polysaccharide: the extracting liquorice cell powder, the distilled water of 15-25 times of weight of adding leaves standstill 5-12h, boiling 1-5 time; Each 1-3h repeats boiling 2-5 time, merging filtrate; Being concentrated to certain volume, is 50-90% with absolute ethyl alcohol modulation final concentration, 0-6 ℃ of hold over night; Remove supernatant,, obtain the licorice polysaccharide powder with sevage method deproteinized;
(2) preparation of honeysuckle concrete: honeysuckle water is extracted 1～3 time, and each amount of water is 10～20 times of medicinal material weight, and extraction time is each 1～3h, and extracting temperature is 50～90 ℃, and the extract concentrate drying is become medicinal extract;
(3) preparation of Chinese date extract: with Removing Kernel for Date, oven dry is pulverized, and adds 10～15 times of water by volume, refluxing extraction 2～3 times, and extraction time is each 1～3h, and extracting temperature is 80～95 ℃, and the merging extract carries out low-press thick and shortens medicinal extract into;
(4) with above step extract and honey as major ingredient, add major ingredient and auxiliary material according to aforementioned proportion, mix, granulate, drying, whole grain promptly obtains licorice polysaccharide honeysuckle date honey granule.
4. according to the preparation method of the said licorice polysaccharide honeysuckle of claim 3 date honey particles agent, it is characterized in that said licorice polysaccharide is to obtain by extracting in the Radix Glycyrrhizae suspension cell, its preparation method is:
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 5%～15% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 200r/min～500r/min, and temperature is 20 ℃～30 ℃, sterilization time 20min～40min; MS contains 2%-5% sucrose in cultivating, and pH 5～6 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 20～28 ℃ of illumination boxs, cultivated 30～35 days, obtain the Radix Glycyrrhizae aseptic seedling;
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable, and the callus subculture medium is the MS culture medium, adds 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH 5～6 before the sterilization, subculture cycle 25～30 days;
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium; Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, cultivate 3～5 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 15～20 days subcultures once, shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃, through the screening of going down to posterity for several times, the clone of final obtained performance excellence;
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor of design voluntarily; Inoculum concentration is 5%～10% fresh weight licorice cell, and the fermentation tank throughput is 200L/min～500L/min, and cultivation and composition are the 3/4MS culture medium; Add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH value are 5～9, cultivation cycle is 15～20 days.
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Cited By (1)
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|---|---|---|---|---|
| CN111718888A (en) * | 2020-06-23 | 2020-09-29 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
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2011
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| CN111718888A (en) * | 2020-06-23 | 2020-09-29 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
| CN111718888B (en) * | 2020-06-23 | 2022-08-19 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
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