CN102511708A - Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof - Google Patents
Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof Download PDFInfo
- Publication number
- CN102511708A CN102511708A CN2011104520656A CN201110452065A CN102511708A CN 102511708 A CN102511708 A CN 102511708A CN 2011104520656 A CN2011104520656 A CN 2011104520656A CN 201110452065 A CN201110452065 A CN 201110452065A CN 102511708 A CN102511708 A CN 102511708A
- Authority
- CN
- China
- Prior art keywords
- honeysuckle
- radix glycyrrhizae
- date
- honey
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 52
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 235000012907 honey Nutrition 0.000 title claims abstract description 29
- 235000006545 Ziziphus mauritiana Nutrition 0.000 title claims abstract description 11
- 244000126002 Ziziphus vulgaris Species 0.000 title claims abstract description 11
- 235000008529 Ziziphus vulgaris Nutrition 0.000 title claims abstract description 11
- 239000008187 granular material Substances 0.000 title claims abstract description 10
- 241000202807 Glycyrrhiza Species 0.000 title claims abstract 17
- 241000205585 Aquilegia canadensis Species 0.000 title claims abstract 13
- -1 Glycyrrhiza polysaccharide Chemical class 0.000 title abstract 7
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000000725 suspension Substances 0.000 claims abstract description 17
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 54
- 235000001453 Glycyrrhiza echinata Nutrition 0.000 claims description 52
- 235000017382 Glycyrrhiza lepidota Nutrition 0.000 claims description 52
- 229940010454 licorice Drugs 0.000 claims description 52
- 150000004676 glycans Chemical class 0.000 claims description 45
- 244000303040 Glycyrrhiza glabra Species 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 238000000605 extraction Methods 0.000 claims description 20
- 239000012141 concentrate Substances 0.000 claims description 19
- 238000001035 drying Methods 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 15
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 15
- 239000005720 sucrose Substances 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 239000000463 material Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 9
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 235000008216 herbs Nutrition 0.000 claims description 7
- 230000005587 bubbling Effects 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 235000020985 whole grains Nutrition 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 5
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 4
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 4
- 238000009835 boiling Methods 0.000 claims description 4
- 235000009508 confectionery Nutrition 0.000 claims description 4
- 239000008101 lactose Substances 0.000 claims description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 4
- 239000002994 raw material Substances 0.000 claims description 4
- 239000000811 xylitol Substances 0.000 claims description 4
- 235000010447 xylitol Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 4
- 229960002675 xylitol Drugs 0.000 claims description 4
- 239000004375 Dextrin Substances 0.000 claims description 3
- 229920001353 Dextrin Polymers 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
- 235000019425 dextrin Nutrition 0.000 claims description 3
- 230000035622 drinking Effects 0.000 claims description 3
- 238000000855 fermentation Methods 0.000 claims description 3
- 230000004151 fermentation Effects 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 230000010354 integration Effects 0.000 claims description 3
- 238000003760 magnetic stirring Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000012879 subculture medium Substances 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- CUZMQPZYCDIHQL-VCTVXEGHSA-L calcium;(2s)-1-[(2s)-3-[(2r)-2-(cyclohexanecarbonylamino)propanoyl]sulfanyl-2-methylpropanoyl]pyrrolidine-2-carboxylate Chemical compound [Ca+2].N([C@H](C)C(=O)SC[C@@H](C)C(=O)N1[C@@H](CCC1)C([O-])=O)C(=O)C1CCCCC1.N([C@H](C)C(=O)SC[C@@H](C)C(=O)N1[C@@H](CCC1)C([O-])=O)C(=O)C1CCCCC1 CUZMQPZYCDIHQL-VCTVXEGHSA-L 0.000 claims description 2
- 235000011477 liquorice Nutrition 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 230000036541 health Effects 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 6
- 230000036039 immunity Effects 0.000 abstract description 4
- 238000012356 Product development Methods 0.000 abstract 1
- 241001570521 Lonicera periclymenum Species 0.000 description 27
- 210000004027 cell Anatomy 0.000 description 17
- 206010013786 Dry skin Diseases 0.000 description 4
- 241001247821 Ziziphus Species 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 125000005909 ethyl alcohol group Chemical group 0.000 description 4
- 235000013402 health food Nutrition 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000011122 softwood Substances 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000001291 vacuum drying Methods 0.000 description 4
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 206010022437 insomnia Diseases 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 229910001369 Brass Inorganic materials 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000208828 Caprifoliaceae Species 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 description 1
- 244000167230 Lonicera japonica Species 0.000 description 1
- 235000017617 Lonicera japonica Nutrition 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 239000010951 brass Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 description 1
- 229960004949 glycyrrhizic acid Drugs 0.000 description 1
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 description 1
- 239000001685 glycyrrhizic acid Substances 0.000 description 1
- 235000019410 glycyrrhizin Nutrition 0.000 description 1
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000036449 good health Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000004957 immunoregulator effect Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof. The glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules are mainly made of, by weight, 8%-12% of glycyrrhiza polysaccharide, 2%-6% of honeysuckle extract, 2%-6% of Chinese date extract, 1%-3% of honey, and 73%-87% of accessories. The health granules mainly made of the glycyrrhiza polysaccharide with the honeysuckle, the Chinese date, the honey and the like bridge the gap of the glycyrrhiza polysaccharide in terms of health product development, and have the effects of immunity improvement and fatigue resistance. The method extracting glycyrrhizae suspension cells to obtain the glycyrrhiza polysaccharide is insusceptible to seasons and regions and is convenient and easy in operation.
Description
Technical field
The present invention relates to a kind of health food and preparation method thereof, particularly a kind of Chinese medicinal granule health food.
Background technology
Radix Glycyrrhizae is as a kind of traditional Chinese medicine; The good reputation of " ten sides, nine grass " is just arranged since ancient times, and the main active of Radix Glycyrrhizae has glycyrrhizic acid, Radix Glycyrrhizae brass and licorice polysaccharide, and the above two have had more deep research; And that the functional study of licorice polysaccharide mainly concentrates on is antitumor; Antiviral, regulate immunocompetence and remove the free radical aspect, seldom in the exploitation of licorice polysaccharide application facet.The Radix Glycyrrhizae suspension cell has more development potentiality as a kind of new approach of licorice polysaccharide of producing, and its licorice polysaccharide content of producing is higher than wild Radix Glycyrrhizae, has significantly anti-oxidant, antiviral, antitumor, immunoregulatory activity; Honeysuckle is caprifoliaceae plant (Lonicera japonica Thunb). the flower that the dry flower of honeysuckle or band are just opened.It is a kind of clinical conventional Chinese medicine, have clearing heat and detoxicating, the effect of wind-heat dissipating.Date traditional Chinese medicine theory is thought; Date has effects such as qi-restoratives benefit gas, nourishing blood and tranquilization, strengthening the spleen and stomach; Be patient's good health care nutriment such as weakness of the spleen and the stomach, insufficiency of vital energy and blood, lassitude, insomnia and dreamful sleep, date is the health food of integration of drinking and medicinal herbs, has very high nutritive value.Honey has a great health care function as a kind of, can not only liver protecting, can regulate nervous system simultaneously, and neurasthenia, insomnia etc. is had a significant effect.At the health products development field, use kind or blank out that the licorice polysaccharide preparation improves immunity.
Summary of the invention
In order to solve the problems of the prior art, the invention provides a kind of licorice polysaccharide honeysuckle date honey particles agent and preparation method thereof, solved the problem of licorice polysaccharide in health products research and development field blank.
The present invention realizes through following technical scheme:
The agent of a kind of licorice polysaccharide honeysuckle date honey particles, primary raw material is to be made up of the honeysuckle of the licorice polysaccharide of method for tissue culture production and integration of drinking and medicinal herbs, date and honey, is aided with other material, wherein primary raw material mass ratio therein is following:
Described carbohydrate auxiliary material is selected from a kind of of lactose, dextrin, sucrose, xylitol or sweet mellow wine, or their mixture.
The preparation method of a kind of licorice polysaccharide honeysuckle date honey particles agent comprises the steps:
(1) extraction of licorice polysaccharide: the extracting liquorice cell powder, the distilled water of 15-25 times of weight of adding leaves standstill 5-12h, boiling 1-5 time, each 1-3h.Repeat boiling 2-5 time, merging filtrate is concentrated to certain volume, is 50-90% with absolute ethyl alcohol modulation final concentration, and 0-6 ℃ of hold over night removed supernatant, with sevage method deproteinized, obtains the licorice polysaccharide powder;
(2) preparation of honeysuckle concrete: honeysuckle water is extracted 1～3 time, and each amount of water is 10～20 times of medicinal material weight, and extraction time is each 1～3h, and extracting temperature is 50～90 ℃, and the extract concentrate drying is become medicinal extract;
(3) preparation of Chinese date extract: with Removing Kernel for Date, oven dry is pulverized, and adds 10～15 times of water by volume, refluxing extraction 2～3 times, and extraction time is each 1～3h, and extracting temperature is 80～95 ℃, and the merging extract carries out low-press thick and shortens medicinal extract into;
(4) with above step extract and honey as major ingredient, add major ingredient and auxiliary material according to aforementioned proportion, mix, granulate, drying, whole grain promptly obtains licorice polysaccharide honeysuckle date honey granule.
Said licorice polysaccharide is to obtain by extracting in the Radix Glycyrrhizae suspension cell, and its preparation method is:
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 5%～15% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 200r/min～500r/min, and temperature is 20 ℃～30 ℃, sterilization time 20min～40min; MS contains 2%-5% sucrose in cultivating, and pH 5～6 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 20～28 ℃ of illumination boxs, cultivated 30～35 days, obtain the Radix Glycyrrhizae aseptic seedling.
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable.The callus subculture medium is the MS culture medium, adds 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH 5～6 before the sterilization, subculture cycle 25～30 days.
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium.Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, cultivate 3～5 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 15～20 days subcultures once.Shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃.Through the screening of going down to posterity for several times, the excellent clone of final obtained performance.
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor (number of patent application 201110255311.9) of design voluntarily; Inoculum concentration is 5%～10% fresh weight licorice cell; The fermentation tank throughput is 200L/min～500L/min; Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH value are 5～9, cultivation cycle is 15～20 days.
Advantage of the present invention is to fill up the blank of licorice polysaccharide aspect the health products exploitation; Proposing a kind of is major ingredient with the licorice polysaccharide; Joining gold honeysuckle flower, date, honey and other substances are developed a kind of health granule of alleviating insomnia, improving immunity, anti-fatigue effect that has simultaneously.Utilize the Radix Glycyrrhizae suspension cell to extract and obtain licorice polysaccharide, this method does not receive the restriction of season and region, and is convenient, easy to operate.
The specific embodiment
Below in conjunction with specific embodiment the present invention is further described.
The preparation of licorice polysaccharide
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 10% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 300r/min, and temperature is 25 ℃, sterilization time 30min; MS contains 3% sucrose in cultivating, and pH 5.9 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 25 ℃ of illumination boxs, cultivated 30 days, obtain the Radix Glycyrrhizae aseptic seedling.
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable.The callus subculture medium is the MS culture medium, adds 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, pH 5.9 before the sterilization, subculture cycle 28 days.
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium.Cultivation and composition are the 3/4MS culture medium, add 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, cultivate 3 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 18 days subcultures once.Shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃.Through the screening of going down to posterity for several times, the excellent clone of final obtained performance.
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor of design voluntarily, inoculum concentration is 8% fresh weight licorice cell, and the fermentation tank throughput is 300L//min, and cultivation and composition are the 3/4MS culture medium, adds 2,4-D (1mgL
-1), NAA (1mgL
-1), 6-BA (0.2mgL
-1), 3% sucrose, pH value are 5.9, cultivation cycle is 18 days.
Embodiment 1:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 20 times of weight, leave standstill 12h; 1h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 3 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 3 times, and each amount of water is 10 times of medicinal material weight, and extraction time is each 1h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 15 times of raw medicinal herbs, and extraction time is each 1.5h, and extracting temperature is 95 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g lactose; 100g sucrose after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, cross 20 eye mesh screens and prepare particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 2:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 15 times of weight, leave standstill 8h; 2h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 2 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 1 time, and each amount of water is 15 times of medicinal material weight, and extraction time is each 3h, and extracting temperature is 90 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 2 times, and each amount of water is 10 times of raw medicinal herbs, and extraction time is each 2.5h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g ethyl cellulose; The 100g xylitol after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 3:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 25 times of weight, leave standstill 5h; 3h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 2 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 2 times, and each amount of water is 20 times of medicinal material weight, and extraction time is each 2h, and extracting temperature is 50 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 12 times of raw medicinal herbs, and extraction time is each 1h, and extracting temperature is 90 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g dextrin; 100g sweet mellow wine after mixing, is crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Embodiment 4:
The extraction of licorice polysaccharide: get 500g licorice cell powder, add the distilled water of 20 times of weight, leave standstill 12h; 1h is extracted in 100 ℃ of water-baths, repeats to extract merging filtrate 3 times; Be concentrated to certain volume, add 4 times of volume absolute ethyl alcohols, 4 ℃ of hold over night; Remove supernatant, with sevage method deproteinized, vacuum drying obtains the licorice polysaccharide powder.
The preparation of honeysuckle concrete: honeysuckle water is extracted 3 times, and each amount of water is 10 times of medicinal material weight, and extraction time is each 1h, and extracting temperature is 80 ℃, and the extract concentrate drying is become medicinal extract.
The preparation of Chinese date extract: date powder water is carried 3 times, and each extracted amount is 15 times of raw medicinal herbs, and extraction time is each 1.5h, and extracting temperature is 95 ℃, and the extract concentrate drying is become medicinal extract.
Get jujube medicinal extract: honeysuckle concrete: the honey quality ratio is that 2: 2: 1 ratio is processed concentrate, gets the 50g licorice polysaccharide, the 300g lactose; 50g sweet mellow wine and 50g xylitol after mixing, are crossed 100 mesh sieves; Add the above-mentioned concentrate of 40ml and mix, process softwood, be placed on the compacting of 20 eye mesh screen left-hand seats and be equipped with particle; 50 ℃ of dryings, the whole grain of 24 mesh sieves promptly obtains the granular preparation of licorice polysaccharide honeysuckle date honey.
Test Example:
Undertaken by the function of enhancing immunity method of inspection in " the health food function assessment assessment process and the method for inspection standard " of Ministry of Public Health's issue.Tried the long-pending 20mL/kg of being of filling body of stomach of thing, every day 1 time, 30d continuously.Adopt the SAS statistical analysis software that data are carried out the average that variance analysis adds the ConA hole with Dunnett ' st check mtt assay (the mouse spleen lymphocyte conversion test that ConA induces) to each group and do not add ConA hole absorbance difference and carry out one-way analysis of variance; Group difference is (F=3.38 significantly; P＜0.05) through Dunnett ' st check, the granular preparation high dose group adds the ConA hole and sees table 1 with the difference average that does not add ConA hole absorbance and remarkable (P＜0.05) result of negative control group comparing difference.
The influence (X SD) of the mouse spleen lymphocyte conversion test that table 1 granular preparation is induced ConA
The SPL conversion test of ConA inducing mouse is the result show, granular preparation has the effect that strengthens the mouse cell immunologic function.
Claims (4)
1. licorice polysaccharide honeysuckle date honey particles agent; It is characterized in that; Primary raw material is to be made up of the honeysuckle of the licorice polysaccharide of method for tissue culture production and integration of drinking and medicinal herbs, date and honey, is aided with other material, and wherein primary raw material mass ratio therein is following:
2. according to the agent of the said licorice polysaccharide honeysuckle of claim 1 date honey particles, it is characterized in that described auxiliary material is selected from a kind of of lactose, dextrin, sucrose, xylitol or sweet mellow wine, or their mixture.
3. the preparation method of licorice polysaccharide honeysuckle date honey particles agent is characterized in that, comprises the steps:
(1) extraction of licorice polysaccharide: the extracting liquorice cell powder, the distilled water of 15-25 times of weight of adding leaves standstill 5-12h, boiling 1-5 time; Each 1-3h repeats boiling 2-5 time, merging filtrate; Being concentrated to certain volume, is 50-90% with absolute ethyl alcohol modulation final concentration, 0-6 ℃ of hold over night; Remove supernatant,, obtain the licorice polysaccharide powder with sevage method deproteinized;
(2) preparation of honeysuckle concrete: honeysuckle water is extracted 1～3 time, and each amount of water is 10～20 times of medicinal material weight, and extraction time is each 1～3h, and extracting temperature is 50～90 ℃, and the extract concentrate drying is become medicinal extract;
(3) preparation of Chinese date extract: with Removing Kernel for Date, oven dry is pulverized, and adds 10～15 times of water by volume, refluxing extraction 2～3 times, and extraction time is each 1～3h, and extracting temperature is 80～95 ℃, and the merging extract carries out low-press thick and shortens medicinal extract into;
(4) with above step extract and honey as major ingredient, add major ingredient and auxiliary material according to aforementioned proportion, mix, granulate, drying, whole grain promptly obtains licorice polysaccharide honeysuckle date honey granule.
4. according to the preparation method of the said licorice polysaccharide honeysuckle of claim 3 date honey particles agent, it is characterized in that said licorice polysaccharide is to obtain by extracting in the Radix Glycyrrhizae suspension cell, its preparation method is:
(1) acquisition of Radix Glycyrrhizae aseptic seedling: select full grains, be bottle-green Radix Glycyrrhizae seed, the clorox with 5%～15% stirs sterilization to seed on magnetic stirring apparatus; Rotating speed is 200r/min～500r/min, and temperature is 20 ℃～30 ℃, sterilization time 20min～40min; MS contains 2%-5% sucrose in cultivating, and pH 5～6 before the sterilization is seeded in the triangular flask that contains MS solid medium 50ml; Be placed in 20～28 ℃ of illumination boxs, cultivated 30～35 days, obtain the Radix Glycyrrhizae aseptic seedling;
(2) acquisition of Radix Glycyrrhizae callus: the Radix Glycyrrhizae callus is to induce from Radix Glycyrrhizae aseptic seedling hypocotyl to get, and after repeatedly screening subculture, growth conditions is stable, and the callus subculture medium is the MS culture medium, adds 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH 5～6 before the sterilization, subculture cycle 25～30 days;
(3) acquisition of Radix Glycyrrhizae suspension cell: select fast growth, loose, the flaxen Radix Glycyrrhizae callus of quality, transfer to after broken with the tweezers folder and carry out suspension cultured in the fluid nutrient medium; Cultivation and composition are the 3/4MS culture medium, add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, cultivate 3～5 days for the first time after, the bulk callus that suspends is removed by filter, suspension cell is every at a distance from 15～20 days subcultures once, shaking speed 110～120rmin
-1, cultivation temperature (20～28) ℃, through the screening of going down to posterity for several times, the clone of final obtained performance excellence;
(4) Radix Glycyrrhizae suspension cell bubbling style reactor is cultivated: utilize the 5L bubbling style reactor of design voluntarily; Inoculum concentration is 5%～10% fresh weight licorice cell, and the fermentation tank throughput is 200L/min～500L/min, and cultivation and composition are the 3/4MS culture medium; Add 2,4-D (0.5～2mgL
-1), NAA (0.5～2mgL
-1), 6-BA (0.1～0.3mgL
-1), 2～5% sucrose, pH value are 5～9, cultivation cycle is 15～20 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104520656A CN102511708B (en) | 2011-12-29 | 2011-12-29 | Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104520656A CN102511708B (en) | 2011-12-29 | 2011-12-29 | Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102511708A true CN102511708A (en) | 2012-06-27 |
| CN102511708B CN102511708B (en) | 2013-08-07 |
Family
ID=46283015
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104520656A Expired - Fee Related CN102511708B (en) | 2011-12-29 | 2011-12-29 | Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102511708B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718888A (en) * | 2020-06-23 | 2020-09-29 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
-
2011
- 2011-12-29 CN CN2011104520656A patent/CN102511708B/en not_active Expired - Fee Related
Non-Patent Citations (5)
| Title |
|---|
| 《中成药》 20080630 陈保华 中药生产的变革__从提取物投料开始 第781-783页 1-4 第30卷, 第06期 * |
| 周斌等: "甘草配方颗粒制备工艺和质量标准研究", 《中成药》 * |
| 张继平等: "超微粉碎技术是促进中药传统产业改造的有效途径", 《中药新药与临床药理》 * |
| 谷丽丽等: "中药超微粉碎技术的应用及进展", 《生命科学仪器》 * |
| 陈保华: "中药生产的变革――从提取物投料开始", 《中成药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718888A (en) * | 2020-06-23 | 2020-09-29 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
| CN111718888B (en) * | 2020-06-23 | 2022-08-19 | 中国医学科学院药用植物研究所 | Culture method for improving glycyrrhizic acid content in suspension culture cells of liquorice |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102511708B (en) | 2013-08-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102578423B (en) | Chinese herbal medicine feed additive for laying hen and preparation method thereof | |
| CN104872758B (en) | The preparation method of cicada fungus burdock oral liquid | |
| CN105105037A (en) | Selenium-rich wheat seedling breakfast replacement powder and preparation method thereof | |
| CN101743854B (en) | Method for preparing morchella mycelium by using dendrobium fiber as substrate | |
| CN105838620A (en) | Ganoderma lucidum culture medium and ganoderma lucidum culture method | |
| CN1059662A (en) | The preparation method of health nutrient | |
| CN108477565A (en) | A kind of lucid ganoderma cream is grown and preparation method thereof | |
| CN100493379C (en) | Method for preparing selenium enriched oyster mushroom powder | |
| CN105754809B (en) | A kind of method and products therefrom of industrialized production organic Se-rich Tibetan cordyceps original plasm wine | |
| CN105193866A (en) | Method for preparing wall-broken caterpillar fungus ultra-fine mixed powder | |
| CN101113430A (en) | Method for gaining panax japonicus secondary metabolite by using panax japonicus cell culture and biological transformation technique | |
| CN102511716A (en) | Glycyrrhiza polysaccharide granules and preparation method thereof | |
| CN106993475A (en) | A kind of lotus pool for Lotus planting | |
| CN102511708B (en) | Glycyrrhiza polysaccharide, honeysuckle, Chinese date and honey granules and preparation method thereof | |
| CN107746304A (en) | A kind of Se-rich lucid ganoderma cultivation matrix and preparation method thereof | |
| CN103932172B (en) | A kind of Chinese caterpillar fungus health product and preparation method thereof | |
| CN103919843B (en) | A kind of liver protection Chinese medicine composition and preparation method thereof | |
| CN109957588A (en) | A kind of fluid nutrient medium inducing radix pseudostellariae cell high yield Pseudostellaria Polysaccharide | |
| CN105030858A (en) | Cynomorium songaricum healthcare tablet and preparation method thereof | |
| CN105851385B (en) | Dendrobium nobile monomer germ tea and preparation method thereof | |
| CN104547604A (en) | Preparation and processing technology of dendrobium candidum capsules | |
| CN104957680A (en) | Chlorella vulgaris health-care tablet and preparation method thereof | |
| CN105942513A (en) | Peony plant edible gum preparing method | |
| CN107684574B (en) | Medicinal and edible composition with blood fat reducing function and preparation method thereof | |
| CN110101075A (en) | A kind of health care oral liquid rich in the lobate layer bacterium of currant |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130807 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |