Background technology
China is drinks production and consumption big country, and in recent years along with growth in the living standard, the chance that people contact drinks and pick-me-up also progressively increases.Because long-term excessive consumption of alcohol or once to take in acute alcoholism and complication that the drinks goods cause in a large number too numerous to enumerate.Research shows the active obviously decline of long-term alcohol user's Gastric Mucosa DH, more is prone to cause the toxic action to liver and body.And if alcohol gets into blood, will combine in vain with red eggs, the normal function of hemoglobin is weakened or lose, cause the delivering oxygen quantity not sufficient, the brain oxygen-supplying amount is reduced and occur going into a coma abnormal mind.Moreover if once excessive drinking makes ethanol timely oxidative metabolism in vivo, ethanol then can produce oxygen radical through enzyme or non-enzyme system, cause lipid peroxidation, and forms MDA.The lipid peroxidation of ethanol can directly damage liver plasma membrane, causes liver a series of dysfunctions to occur.Alcohol dehydrogenase (ADH) is extensive narrow spectrum zinc-containing metal enzyme, is one of oxidation reduction catalyst important in the organism.In people and mammalian body, alcohol dehydrogenase is participated in alcohol metabolism in the body.With NAD is coenzyme, and alcohol catalysis is degraded to acetaldehyde, is metabolic enzyme important in the humans and animals body.If this enzymatic activity descends or is suppressed, body is improved the bioavilability of ethanol, and then increase the weight of the damaging effect of ethanol organs such as brain, livers.Therefore, the activity of raising alcohol dehydrogenase can be alleviated the toxic action of ethanol to body.
The basic way of preventing drunkenness is that the wine that drink is gone into decomposes rapidly in stomach earlier, reduces alcohol as far as possible and gets into blood circulation system, to reduce the burden to liver.This technological key is how to improve the palliating degradation degree of alcohol in stomach, however the alcohol dehydrogenase limited amount that in stomach, exists, and activity is not high.
The alcohol product great majority of separating in the market are simple Chinese medicine, enzyme preparation, chemicals etc.Chinese medicine series products complicated component, active ingredient is indeterminate, and using dosage is uncertain, to the different effects that individuality played very big-difference is arranged; And simple enzyme preparation is harsh to environmental requirement, and cost is too high, and general consumer is difficult to bear, and takes that the hydrochloric acid in gastric juice of human body can make its part enzyme deactivation, DeGrain after this enzyme preparation; The relieve the effect of alcohol composition of medicine of most of chemistry is excitant, hormone, amino acid etc., take can occur in the short time after these compositions clear-headed, excited, alleviate state of intoxication, still take for a long time health is damaged.
Technical scheme
The purpose of this invention is to provide a kind of preventing drunkenness product, it can improve the content of the alcohol dehydrogenase in the human body, and further activates alcohol dehydrogenase its accelerating alcohol is decomposed.
The technical scheme that the present invention adopted is: a kind of preventing drunkenness product, its active ingredient are alcohol dehydrogenase, xylo-oligosaccharide and citrus fruit extract.
Further say, contain in every dose of described preventing drunkenness product: alcohol dehydrogenase 0.1~0.8g, xylo-oligosaccharide 1~5g, citrus fruit extract 0.5~5g.Every dose, refer to the single dose.Above-mentioned dosage is the human body calculating about 50 kilograms with body weight, and when specifically taking, the consumer possibly take the circumstances into consideration to increase or reduce dose according to factors such as drunk situation and body weight.In the preferred version, contain alcohol dehydrogenase 0.2~0.4g xylo-oligosaccharide 0.7~2.8g in the preventing drunkenness product, citrus fruit extract 0.5~2.4g.
Preferably, also contain raisin tree seed extract in the described preventing drunkenness product.
Further, contain in every dose of described preventing drunkenness product: xylo-oligosaccharide 0.7~2.8g, citrus fruit extract 0.5~2.4g, raisin tree seed extract 0.2~0.8g, Cobastab
20.001~0.008g, nicotinic acid 0.001~0.008g, dextrin 0.16~1.9g, alcohol dehydrogenase 0.2~0.4g and auxiliary material.
Preferably, described auxiliary material comprises stabilizing agent.
Preferably, described citrus fruit comprises shaddock, mandarin orange, tangerine, orange, lemon.
Preferably, in the described preventing drunkenness product, the mass ratio of described xylo-oligosaccharide and citrus fruit extract is 0.33~3: 1, and the weight ratio of the composition of xylo-oligosaccharide and citrus fruit extract and alcohol dehydrogenase is 4~36: 1.
Preferably, described preventing drunkenness product comprises beverage, tablet, electuary.
The present invention is through adding the alcohol dehydrogenase of certain ingredients; Replenish the deficiency of alcohol dehydrogenase in the stomach; And xylo-oligosaccharide and Citrus extract can activate the activity that improves alcohol dehydrogenase significantly, thereby ethanol decomposes the purpose that plays Dealcoholic sobering-up in the acceleration bodies, and the process that ethanol is decomposed is carried out under one's belt; Decompose thereby avoid ethanol to be absorbed getting into blood finally to get into liver, effectively reduce the harm of alcohol liver.
Another object of the present invention provides the new application of the composition of a kind of xylo-oligosaccharide and citrus fruit extract.
The technical scheme that the present invention adopts is that the composition with xylo-oligosaccharide and citrus fruit extract is applied in the preventing drunkenness product.
Preferably, in every dose of described preventing drunkenness product, the administered dose of xylo-oligosaccharide is 1~5g, and the administered dose of citrus fruit extract is 0.5~5g.That is, taking way is: single-dose dosage xylo-oligosaccharide is 1~5g, citrus fruit extract 0.5~5g (calculate with 50KG body weight human body, the consumer possibly take the circumstances into consideration to increase or reduce dose according to factors such as drunk situation and body weight).In the preferred version, contain alcohol dehydrogenase 0.2~0.4g xylo-oligosaccharide 0.7~2.8g in every dose of preventing drunkenness product, citrus fruit extract 0.5~2.4g.
Further, described preventing drunkenness product contains xylo-oligosaccharide 0.7~2.8g in every dose, citrus fruit extract 0.5~2.4g, raisin tree seed extract 0.2~0.8g, Cobastab
20.001~0.008g, nicotinic acid 0.001~0.008g, dextrin 0.16~1.9g, the dosage of alcohol dehydrogenase 0.2~0.4g gives.
Preferably, the adding proportion of xylo-oligosaccharide described in the described preventing drunkenness product and citrus fruit extract is 0.33~3: 1 (mass ratio).
The effect of each component is following in the above-mentioned preventing drunkenness product:
Alcohol dehydrogenase (ADH): alcohol dehydrogenase can be taken off two hydrogen atoms in the alcohol molecule, ethanol is decomposed become acetaldehyde, and then catabolism is carbon dioxide and water.If this kind of enzyme is active strong in the body, ethanol can be degraded rapidly, and it is not liquor-saturated to drink.But most of people are the Asia yellow especially, and this enzymatic activity is not strong, shows as capacity for liquor very.Therefore the purpose of relieving the effect of alcohol fast to be reached, the alcohol dehydrogenase of external source must be added.
Xylo-oligosaccharide: the activity to ADH (alcohol dehydrogenase) has tangible activation, and when xylo-oligosaccharide concentration was 0.71g/L, activity ratio was 26.91%.Only need consumption seldom, just can significantly increase the activity of alcohol dehydrogenase, the ethanol content in the body of degrading fast, thus reduction alcohol is to harm that human body produced.In addition, excessive drinking can cause gut flora seriously disorderly, and the bacterium by the generation of alcohol toxin that assembles in the small intestine may cause the malabsorption of fat, carbohydrate, protein, folic acid and VB.And xylo-oligosaccharide can promote the rapid propagation of Bifidobacterium in the enteron aisle; Accelerate the wriggling and the emptying of enteron aisle, the eliminating speed of acceleration of alcohol and metabolite thereof reduces the absorption of Ammonia material; Reduction is played the effect of relieving alcoholism and protecting liver from many aspects because of the hepatic coma phenomenon that ammonia concentration raises and causes.
Citrus extracts: the citrus fruit extract contains anti-oxidants such as abundant carrotene, ascorbic acid, can remove the oxygen radical in the large intestine; Vitamin C in the oranges and tangerines is 7 times of apple, 10 times of pears, can suppress the activity of acetaldehyde, eliminates drunk arch-criminal; Effects such as that flavonoids has is anti-oxidant, anticancer, reducing blood lipid, reduction cholesterol; Lemon bitter can the kill harmful bacterium, the level of antiviral, antalgic and inflammation relieving, anticancer and control agent inner cholesterol, prevent atheroma; Distinctive citrin has the effect of regulating osmotic pressure, strengthening the capillary resistance.Enrich vitamin and active material in the citrus fruit extract and also have harmonizing intestine-stomach, help digest, liver-protective effect.
Hoveniae semoveniae semen: contain a large amount of glucose, organic acid in the hoveniae semoveniae semen, can expand the blood volume of human body, again can relieving alcoholism; So the effect of sobering up and calming the nerves is arranged, can treat the chest diaphragm dysphoria with smothery sensation due to the insobriety, a wind; Underbelly is arrested anxious, and is thirsty vexed, illnesss such as difficulty in urination and defecation.
Vitamin B2: with the metabolism of fat confidential relation is arranged, can decompose the fat of piling up in the liver of causing, strengthen liver function, avoid occurring fatty liver by acetaldehyde.
Nicotinic acid: passing hydrogen vitamin, is the precursor of DPN, plays dehydrogenation and the effect of transmitting hydrogen in vivo, is the core substance of dehydrogenase, strengthens the decomposition effect of ethanol, acetaldehyde.
Dextrin: can the preventing drunkenness product be processed accompanied with sticky preparation in various degree, can on gastric mucosa, form diaphragm, slow down the speed that gastric mucosa absorbs alcohol.
Above-mentioned component is combined, and the deficiency that can replenish alcohol dehydrogenase in the stomach significantly improves the activity of alcohol dehydrogenase, accelerating alcohol, acetaldehyde decomposition and inversion in vivo; Can promote the rapid propagation of Bifidobacterium in the enteron aisle again, accelerate the wriggling and the emptying of enteron aisle, the eliminating speed of acceleration of alcohol and metabolite thereof is accelerated fat metabolism, removes interior free yl.Have prevent drunk, relieve the effect of alcohol, safeguard after drinking microecological balance in the body fast, regulate stomach, liver-protective effect.
Use Wa Le-Huo He (Valle&Hoch) body of laws and measure the influence of xylo-oligosaccharide outward alcohol dehydrogenase activity.
1 experiment material:
1.1 reagent
NAD (NAD+) (originating from Roche company) biochemistry level, the biochemical level of alcohol dehydrogenase (ADH) (originating from Sigma company), xylo-oligosaccharide (originating from Suzhou wealthy bio tech ltd earlier), it is pure that other reagent are analysis, and water is purified water.
1.2 laboratory apparatus
Day island proper Tianjin UV-1700 type ultraviolet specrophotometer, the PHS-2F of Shanghai Precision Scientific Apparatus Co., Ltd type pH meter.
2 formulations prepared from solutions
2.1 Tris (trishydroxymethylaminomethane)-hydrochloride buffer: get 12.11gTris and be dissolved in about 80ml water, transfer pH to 8.0 with hydrochloric acid, and be settled to 100ml.
2.2 substrate solution (ethanolic solution): it is water-soluble to get the 11.5ml absolute ethyl alcohol, and is settled to 100ml.
2.3 coenzyme solution: it is water-soluble to get the 0.16g NAD, and is settled to 10ml.
2.4 enzyme solutions: it is water-soluble to get 10 μ lADH enzyme liquid (2030U/ml), and is settled to 10ml.
2.5 xylo-oligosaccharide solution: the xylo-oligosaccharide solution of preparation 22.68g/L.
2.6 citrus fruit extract solution: the sweet orange fruit powder solution of preparation 22.68g/L.
3 assay methods
3.1 principle
Wa Le-Huo He (Valle&Hoch) body of laws is measured xylo-oligosaccharide outward to alcohol dehydrogenase activity.
ADH is in the weak base scope that has NAD (NAD+) to exist, and the catalysis ethanol dehydrogenation reaction generates acetaldehyde.In course of reaction, NAD+ can become its reduced form (NADH), and this variation is different with the difference of alcohol dehydrogenase activity.NADH has maximum absorption band in spectrophotometer 340nm wavelength, can follow the tracks of this reaction through the increase situation that detects 340nm place absorbance.
Reaction equation is following:
Abridge in the formula:
β-NAD: β-NADH, oxidized form
β-NADH: β-NADH, reduced form
3.2 step:
A, preparing experiment group:
See table 1, in pipe to be measured, add Tris-hydrochloride buffer 1.4ml, NAD+1ml, ethanolic solution 0.5ml, xylo-oligosaccharide solution 0.1ml, citrus fruit extract solution 0.1ml at 25 ℃ of following mixings, adds alcohol dehydrogenase 0.1ml;
Add Tris-hydrochloride buffer 1.4ml in the background pipe, NAD+1ml, ethanolic solution 0.5ml does not add xylo-oligosaccharide, citrus fruit extract solution and ADH enzyme liquid;
Add Tris-hydrochloride buffer 1.4ml in the blank pipe, NAD+1ml, ethanolic solution 0.5ml, alcohol dehydrogenase 0.1ml do not add xylo-oligosaccharide and citrus fruit extract solution;
Contrast 1 group and add Tris-hydrochloride buffer 1.4ml, NAD+1ml, ethanolic solution 0.5ml, xylo-oligosaccharide solution 0.1ml, alcohol dehydrogenase 0.1ml do not add the citrus fruit extract
Contrast 2 groups and add Tris-hydrochloride buffer 1.4m l, NAD+1ml, ethanolic solution 0.5ml, citrus fruit extract solution 0.1ml, alcohol dehydrogenase 0.1ml do not add xylo-oligosaccharide
Blank pipe, background pipe, contrast 1 group and contrast 2 groups and supply with pure water respectively.Use spectrophotometric determination absorbance (A340 value) behind the mixing immediately, whenever once at a distance from the 1s reading, METHOD FOR CONTINUOUS DETERMINATION 5min.
Table 1 xylo-oligosaccharide and citrus fruit extract are to the active influence mensuration system of ADH
According to formula
Active activation/the inhibiting rate (%) of ADH=(△ A
Sample-△ A
Blank)/(△ A
Blank-△ A
Background) * 100% calculates xylo-oligosaccharide solution and the citrus fruit extract activation/inhibitory action to ADH.
4 experimental results
Above-mentioned every kind of test all repeats 5 times, and experimental data is seen table 2-table 5.
Table 2 xylo-oligosaccharide and citrus fruit extract are to the active influence mensuration-blank pipe of ADH
Table 3 xylo-oligosaccharide and citrus fruit extract are to the active influence mensuration-control tube 1 (xylo-oligosaccharide is only arranged) of ADH
Table 4 xylo-oligosaccharide and citrus fruit extract are to the active influence mensuration-control tube 2 (the citrus fruit extract is only arranged) of ADH
Table 5 xylo-oligosaccharide and citrus fruit extract are to the active influence mensuration-pipe to be measured of ADH
The experimental data of surveying represent with
, according to the active activation/inhibiting rate formula of ADH computing activation/inhibiting rate.Experimental result is seen table 6.
Table 6 xylo-oligosaccharide solution is to the active influence of ADH
Can be found out that by experimental result xylo-oligosaccharide and citrus fruit extract have activation to alcohol dehydrogenase respectively, when not having the citrus fruit extract when xylo-oligosaccharide (0.71g/L) is only arranged, activity ratio is 26.91%; When not having xylo-oligosaccharide when citrus fruit extract (0.71g/L) is only arranged, activity ratio is 7.88%; When above-mentioned both when existing simultaneously, activity ratio is 36.65%, and is higher than independent xylo-oligosaccharide or independent citrus fruit extract activity ratio.
Make the present invention compare with the existing health food that relieves the effect of alcohol through technique scheme and have following advantage: xylo-oligosaccharide and citrus fruit extract combine that alcohol dehydrogenase is had tangible activation; Its action effect is better than independent use xylo-oligosaccharide or citrus fruit extract; Compare with other simple antialcoholic drugs that add enzyme preparation; Can make alcohol dehydrogenase have higher activity; Therefore it is few, rapid-action to have consumption, and the advantage of the ethanol content in the body capable of being fast degraded reduces alcohol to harm that human body produced.