CN102499066A - Red leaf marked hybrid cotton and method for identifying purity, counterfeiting and generation by red leaf marked hybrid cotton - Google Patents

Red leaf marked hybrid cotton and method for identifying purity, counterfeiting and generation by red leaf marked hybrid cotton Download PDF

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CN102499066A
CN102499066A CN2011103704101A CN201110370410A CN102499066A CN 102499066 A CN102499066 A CN 102499066A CN 2011103704101 A CN2011103704101 A CN 2011103704101A CN 201110370410 A CN201110370410 A CN 201110370410A CN 102499066 A CN102499066 A CN 102499066A
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cotton
hybrid
autumnal leaves
red autumnal
generation
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CN102499066B (en
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赵永民
纪家华
王义平
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SHANDONG NONGXING PLANTING INDUSTRY Co Ltd
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SHANDONG NONGXING PLANTING INDUSTRY Co Ltd
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Abstract

The invention belongs to the field of agricultural breeding and particularly relates to red leaf marked hybrid cotton and a method for identifying the purity, the counterfeiting and the generation by the red leaf marked hybrid cotton. An F1-generation hybrid, i.e. the red leaf marked hybrid cotton, is prepared by carrying out hybrid seed production by using an upland cotton red leaf stigma exsertion line 96-67 or an insect-resistant upland cotton red leaf stigma exsertion line 00-36 as a male parent and using an insect-resistant upland cotton parent L16-6 as a female parent. The red leaf marked hybrid cotton and the method have the advantages that a bred inbred line parent has the characteristics of high comprehensiveness and high combining ability and the marked hybrid screened by the bred inbred line parent has excellent performances. The red leaf marked hybrid cotton can be used for directly judging the purity of the hybrid according to the red leaf marked characters, removing abnormal plants and ensuring the advantages of the hybrid to be completely played and can be used for judging the identifying and the generation of hybrid according to the separation ratio of the characters.

Description

Red autumnal leaves mark hybrid cotton and purity thereof, true and false and the generation other authentication method
Technical field
The invention belongs to the agricultural breeding field, be specifically related to a kind of red autumnal leaves mark hybrid cotton and purity thereof, true and false and the generation other authentication method.
Background technology
Hybrid vigour is the universal phenomenon of biosphere, and cotton is no exception.Mell delivered first piece about the report of cotton hybrid than its participation hybrid strain increase economical character and some determination datas of fiber properties in 1894, and Cook (1909) is that first proposition utilizes sea-island cotton * upland cotton heterotic.Comprehensive former research results can be found out; Cotton crossbreed can be coordinated high yield, high-quality, proterties such as disease-resistant in one preferably; Better make up hybrid F1 lint yield competitive advantage between the upland cotton kind at 10-30%; Average 20%, also have up to 50%-138% and even surpass 200% report in India.
After the fifties hybrid vigour was got off by people certainly from last century, the world respectively produced cotton state and has all carried out heterosis utilization work.India utilizes success of hybrid vigour and the maximum country of area in the world.China at first utilized hybrid vigour in Henan from 1976, excellent number of the Bohai Sea of comparatively successful the is former Huimin Prefecture institute of agricultural sciences, Sichuan Province cotton assorted number of river and middle cotton institute in mix number.Have large quantities of bollworm-resistance hybrid cottons on producing, to apply in the recent period again, with in 29, cotton No. 15 of Shandong etc. is the most successful.Facts have proved; Utilize hybrid vigour to have in the production and can unify to supply to plant, improve the raw cotton yield and quality effectively, can protect advantages such as breeding units intellectual property preferably; Have vast potential for future development, be considered to break pace up and down valid approach of situation of present output of cotton.
During cotton crossbreed is produced at present; Using the cotton parent, all is the parent of the general type of greenery generally, does not have the mark property that can intuitively distinguish; In the purity of cotton crossbreed, true and false and generation, can't do not distinguished according to proterties directly perceived; Therefore, be with economic loss to the cotton grower often, the also bad protection of breeder's rights and interests.
Summary of the invention
The purpose of this invention is to provide other authentication method of a kind of red autumnal leaves mark hybrid cotton and purity thereof, true and false and generation, both had the merit of hybrid cotton, the purity, true and false and for other authentication method of this hybrid cotton can be provided again intuitively.
A kind of red autumnal leaves mark hybrid cotton of the present invention is obtained by following preparation method:
(1) with anti-red autumnal leaves parent of upland cotton and stigma appearing germplasm line 92-42 hybridization, at F 2Select the plant have red autumnal leaves and stigma appearing proterties simultaneously in generation, in 3~4 generations of continuous selfing, breed upland cotton red autumnal leaves stigma appearing series 96-67;
(2) red autumnal leaves stigma appearing germplasm line 96-67 and the Insect Resistant Cotton GK-12-5 hybridization selected with step (1) are at first to F 2Carry out resistance to insects for colony and identify, the plant that then selection has red autumnal leaves and stigma appearing proterties simultaneously in pest-resistant colony, in 3~4 generations of selfing, also carried out the resistance to insects evaluation continuously, breeds pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36;
(3) cotton is ground in 16 in the Insect Resistant Cotton Shandong, selects fine individual plant, standard be boll per plant more than 15, disease-resistant, fibre length 29.0mm above, ginning outturn 38% or more, bell weigh 5.0 restrain more than.To above individual plant 3~4 generations of continuous selfing and carry out resistance to insects and identify, breed pest-resistant upland cotton parent L16-6;
(4) the pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36 that utilizes upland cotton red autumnal leaves stigma appearing series 96-67 that step (1) selects or step (2) to select is the pest-resistant upland cotton parent L16-6 that male parent and step 3 select and does the hybridization of female parent production of hybrid seeds, obtains F 1Be red autumnal leaves mark hybrid cotton for crossbreed.
Wherein, all preferably resist characteristic of disease, yield traits and quality trait in the selfing process in step (1)~(3) and screen, wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 13, Dan Lingchong >=4.5 grams, ginning outturn are standard more than 38%; Quality trait is selected 2.5% span length >=29.0mm according to HVI900 series of tests result, specific strength >=28.0cN/tex, mic value 3.5~5.0.
Resistance to insects authentication method in step (2) and the step (3) is preferably following: grow into 1~3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
Red autumnal leaves mark hybrid cotton purity authentication method of the present invention; The crossbreed that produces in the step (4) planted in the land for growing field crops or with incubator cultivate, after cotyledon is open and flat, identify according to the cotyledon color; The leaf look of true crossbreed is red, and the leaf look of false crossbreed is green.
The true and false authentication method of red autumnal leaves mark hybrid cotton of the present invention, the F of the crossbreed that produces in the results steps (4) 1In generation, carries out selfing and obtains F 2In generation, observe F 2Dai Yese and flower portion proterties segregation ratio, the ratio of true crossbreed is redness: green=3: 1, normal bud: stigma appearing bud=3: 1.
Red autumnal leaves mark hybrid cotton other authentication method of generation of the present invention, the crossbreed that produces in the step (4), F 1In generation, do not produce separation, and the leaf look is red, and flower portion proterties is identical with normal cotton, F 2Dai Yese produces with flower portion proterties and separates, and the ratio of true crossbreed is redness: green=3: 1, and normal bud: stigma appearing bud=3: 1.
The anti-red autumnal leaves parent of upland cotton of the present invention, stigma appearing germplasm line 92-42, upland cotton red autumnal leaves stigma appearing series 96-67, Insect Resistant Cotton GK-12-5, pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36, Insect Resistant Cotton Shandong cotton grind 16 and pest-resistant upland cotton parent L16-6 all purchase in emerging kinds of Shandong farming Co., Ltd already.
The invention has the advantages that: the inbred line parent who selects has comprehensive good, characteristics that coordinate force is high, and is good with its marking type crossbreed that filters out performance.This red autumnal leaves mark hybrid cotton can directly be judged the purity of cenospecies based on the red autumnal leaves mark property, removes assorted strain, and 100% guarantees heterotic performance, and can be based on the segregation ratio of proterties, and increase of judgement cenospecies and generation are not.
Embodiment
Below in conjunction with embodiment the present invention is further specified.
Embodiment 1:
Prepare red autumnal leaves mark hybrid cotton according to following preparation method:
In July, (1) first, with anti-red autumnal leaves parent of upland cotton and stigma appearing germplasm line 92-42 hybridization, plant F in Hainan winter 1Generation, 1 year, at F 2Select to have simultaneously 22 of the plant of red autumnal leaves and stigma appearing proterties in generation, educated through Nan Fanbei in the 3rd year~the 4th year, in 3~4 generations of continuous selfing, the 5th year, breed upland cotton red autumnal leaves stigma appearing series 96-67.13.7 of upland cotton red autumnal leaves stigma appearing series 96-67 boll per plants, ginning outturn 40.7%, bell weighs 4.62 grams, 2.5% span length 30.0mm, specific strength 34.5cN/tex, mic value 5.0.
(2) the 5th years, with red autumnal leaves stigma appearing germplasm line 96-67 and Insect Resistant Cotton GK-12-5 hybridization, planted F in Hainan winter 1Generation, the 6th year, at first to F 2Carrying out resistance to insects for colony identifies; The plant that then selection has red autumnal leaves and stigma appearing proterties simultaneously in pest-resistant colony; In the 7th year~the 8th year continuous 4 generations of selfing, also carried out resistance to insects and identify, the 9th year, bred pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36.Pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36 high resistance helicoverpa armigera, 13.8 of boll per plants, ginning outturn 39.2%, bell weighs 4.51 grams, 2.5% span length 29.2mm, specific strength 30.5cN/tex, mic value 4.8.
(3) the 7th years~the 9th year, cotton was ground in 16 in the Insect Resistant Cotton Shandong, selects fine individual plant, and continuous 3~4 generations of selfing also carry out resistance to insects and identify, breed pest-resistant upland cotton parent L16-6.Pest-resistant upland cotton parent L16-6 high resistance helicoverpa armigera, 13.8 of boll per plants, ginning outturn 42.2%, bell weighs 5.48 grams, 2.5% span length 29.0mm, specific strength 30.5cN/tex, mic value 4.4.
(4) the tenth years~the 11 year; Utilize upland cotton red autumnal leaves stigma appearing series 96-67 to do the hybrid combination improved crossing kind that male parent and pest-resistant upland cotton parent L16-6 do hybridization of female parent; In the 12 year~the 13 year; Unginned cotton, gined cotton, the preceding gined cotton of frost are 238.4 kilograms, 221.8 kilograms, 92.4 kilograms and 86.2 kilograms before two annual per mu yield unginned cottons, the frost, respectively than No. 29 raising the output 8.9%, 8.0%, 6.2% and 5.6% of middle cotton institute; 237.1 kilograms, 227.0 kilograms, 97.4 kilograms and 93.4 kilograms of gined cottons before unginned cotton, gined cotton, the frost grind No. 15 raising the output 2.2%, 2.7%, 1.4% and 2.0% than Shandong cotton respectively before the 16 year production test average yield per mu unginned cotton, the frost.This crossbreed characteristic is to belong to the mid-early maturity kind.Emerge better, the time of infertility, growing way was sane, medium blade size, red.Regional test result: 129 days breeding times, 101 centimetres of plant heights, the plant turriform, 7.1 of first fruit branch joint positions, several 13.1 of fruit branch, 18.2 of boll per plants, bell weigh 6.1 grams, bell oval.Ginning outturn 39.0% before the frost, seed refer to 10.9 grams, flower rate 93.9% before the frost, stiff hemp nettle rate 5.6%.The 12 year and the 13 year are through test (HVICC): the fibre length 30.7mm of cotton quality supervision and inspection center of the Ministry of Agriculture, specific strength 30.7cN/tex, mic value 4.6, regularity 84.3%, spinning evenness index 144.2.Shandong Cotton Research Center is disease-resistant, resistance to insects is identified: anti-fusarium wilt, anti-verticillium wilt, high resistance helicoverpa armigera.
Wherein, in order to improve the seed selection effect, all resist characteristic of disease, yield traits and quality trait in the selfing process in step (1)~(3) and screen, wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 13, Dan Lingchong >=4.5 grams, ginning outturn are standard more than 38%; Quality trait is selected 2.5% span length >=29.0mm according to HVI900 series of tests result, specific strength >=28.0cN/tex, mic value 3.5~5.0.In order to guarantee parent's resistance to insects; Resistance to insects authentication method in step (2) and step (3) is: grow into 1~3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
Purity of hybrid, true and false, generation other authentication method: the 12 year, the crossbreed that produces in (4) set by step, the 13 year plantation F 1In generation, the leaf look all is red, and flower portion proterties is identical with normal cotton does not see and separate.The 14 year, plantation F 2Generation, observe 400 strain leaf looks and flower portion proterties segregation ratio continuously in the land for growing field crops, the result is redness: green=302: 98, normal bud: stigma appearing bud=298: 102, segregation ratio meets 3: 1.
Embodiment 2:
Step among the embodiment 1 (4) is changed into pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36 as male parent, and all the other are identical with embodiment 1.The improved crossing kind, in Shandong Province's cotton preliminary experiment, gined cotton is 267.1 kilograms, 252.6 kilograms, 107.1 kilograms and 100.6 kilograms before average yield per mu unginned cotton, the preceding unginned cotton of frost, gined cotton, the frost.This crossbreed characteristic is to belong to the mid-early maturity kind.Emerge, the time of infertility, growing way was sane, medium blade size, red.Regional test result: 124 days breeding times, 105.1 centimetres of plant heights, the plant turriform, 7.0 of first fruit branch joint positions, several 14.1 of fruit branch, 21.6 of boll per plants, bell weigh 6.7 grams, bell oval.Ginning outturn 39.8% before the frost, seed refer to 10.8 grams, flower rate 96.4% before the frost, stiff hemp nettle rate 5.3%.Test (HVICC): the fibre length 30.7mm of cotton quality supervision and inspection center of the Ministry of Agriculture, specific strength 30.4cN/tex, mic value 4.8, regularity 85.9%, spinning evenness index 151.5.Shandong Cotton Research Center is disease-resistant, resistance to insects is identified: anti-fusarium wilt, anti-verticillium wilt, high resistance helicoverpa armigera.

Claims (6)

1. red autumnal leaves mark hybrid cotton is characterized in that being obtained by following preparation method:
(1) with anti-red autumnal leaves parent of upland cotton and stigma appearing germplasm line 92-42 hybridization, at F 2Select the plant have red autumnal leaves and stigma appearing proterties simultaneously in generation, in 3~4 generations of continuous selfing, breed upland cotton red autumnal leaves stigma appearing series 96-67;
(2) red autumnal leaves stigma appearing germplasm line 96-67 and the Insect Resistant Cotton GK-12-5 hybridization selected with step (1) are at first to F 2Carry out resistance to insects for colony and identify, the plant that then selection has red autumnal leaves and stigma appearing proterties simultaneously in pest-resistant colony, in 3~4 generations of selfing, also carried out the resistance to insects evaluation continuously, breeds pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36;
(3) cotton is ground in 16 in the Insect Resistant Cotton Shandong; Select fine individual plant; Standard be boll per plant more than 15, disease-resistant, fibre length 29.0mm above, ginning outturn 38% or more, bell weigh more than 5.0 grams, to above individual plant 3~4 generations of continuous selfing and carry out the resistance to insects evaluation, breeds pest-resistant upland cotton parent L16-6;
(4) the pest-resistant upland cotton red autumnal leaves stigma appearing series 00-36 that utilizes upland cotton red autumnal leaves stigma appearing series 96-67 that step (1) selects or step (2) to select is the pest-resistant upland cotton parent L16-6 that male parent and step (3) select and does the hybridization of female parent production of hybrid seeds, obtains F 1Be red autumnal leaves mark hybrid cotton for crossbreed.
2. red autumnal leaves mark hybrid cotton according to claim 1; It is characterized in that all resisting characteristic of disease, yield traits and quality trait in the selfing process in step (1)~(3) screens; Wherein, disease resistance is carried out in sick naturally garden, selects good individual plant of anti-blight or plant; Yield traits is investigated knot bell property, and under per hectare 4.5 ten thousand strain density, boll per plant more than 13, Dan Lingchong >=4.5 grams, ginning outturn are standard more than 38%; Quality trait is selected 2.5% span length >=29.0mm according to HVI900 series of tests result, specific strength >=28.0cN/tex, mic value 3.5~5.0.
3. red autumnal leaves mark hybrid cotton according to claim 1; It is characterized in that the resistance to insects authentication method in step (2) and the step (3) is: grow into 1~3 leaf period at cotton seedling; Kanamycin sulfate solution with 4% spreads upon on the seedling leaves; Observe after seven days and smear blade, the seedling of blade flavescence is extracted, keep the cotton seedling of normal growth.
4. the described red autumnal leaves mark of claim 1 hybrid cotton purity authentication method; It is characterized in that the crossbreed that produces in the step (4) planted in the land for growing field crops or with incubator and cultivate; After cotyledon is open and flat; Identify that according to the cotyledon color leaf look of true crossbreed is red, the leaf look of false crossbreed is green.
5. the true and false authentication method of the described red autumnal leaves mark of claim 1 hybrid cotton is characterized in that gathering in the crops the F of the crossbreed that produces in the step (4) 1In generation, carries out selfing and obtains F 2In generation, observe F 2Dai Yese and flower portion proterties segregation ratio, the ratio of true crossbreed is redness: green=3: 1, normal bud: stigma appearing bud=3: 1.
6. the described red autumnal leaves mark of claim 1 a hybrid cotton other authentication method of generation is characterized in that the middle crossbreed that produces of step (4), F 1In generation, do not produce separation, and the leaf look is red, and flower portion proterties is identical with normal cotton, F 2Dai Yese produces with flower portion proterties and separates, and the ratio of true crossbreed is redness: green=3: 1, and normal bud: stigma appearing bud=3: 1.
CN201110370410.1A 2011-11-21 2011-11-21 Red leaf marked hybrid cotton and method for identifying purity, counterfeiting and generation by red leaf marked hybrid cotton Expired - Fee Related CN102499066B (en)

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CN103477972A (en) * 2013-09-13 2014-01-01 山东农兴种业有限责任公司 Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof
CN103999766A (en) * 2014-05-26 2014-08-27 中棉种业科技股份有限公司 Breeding method for identifying purity of hybrid cotton in early stage

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Publication number Priority date Publication date Assignee Title
CN103477972A (en) * 2013-09-13 2014-01-01 山东农兴种业有限责任公司 Red leaf marking restoring line and three-line crossbreed selection and breeding method thereof
CN103477972B (en) * 2013-09-13 2016-04-27 山东农兴种业有限责任公司 A kind of red autumnal leaves mark restorer and three line hybrid seed selection thereof
CN103999766A (en) * 2014-05-26 2014-08-27 中棉种业科技股份有限公司 Breeding method for identifying purity of hybrid cotton in early stage

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