CN102488828B - Chinese medicinal composition for treating infectious bronchitis and its preparation method - Google Patents
Chinese medicinal composition for treating infectious bronchitis and its preparation method Download PDFInfo
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Abstract
The invention discloses a Chinese medicinal composition for treating infectious bronchitis and its preparation method. The Chinese medicinal composition is prepared from vietnamese sophora roots, Rhizoma Belamcandae, Rhizoma Anemarrhenae, gypsum, Herbal Ephedrae, almonds and Radix glycyrrhizae in certain proportion. With simple formula, low cost and stable quality, the Chinese medicinal composition can be used to effectively treat infectious bronchitis. Compared with the control group of Maxingshigan decoction, the prescription of the composition has mortality decreased by 6.9%-24%, a cure rate enhanced by 6%-26.7%, and an effective rate increased by 6.9%-24%; and compared with an infected control group, the mortality is reduced by 18.2%-35.3%. Through comprehensive judgment on the characteristic lesion scores of tracheas and kidneys, the composition has good improving effects on pathological damages in chickens infected with IBV (infectiousbronchitis virus), and it not only has definite curative effects, but also has no toxic or side effect and drug residue. While improving breeding benefits, the composition also avoids causing harm to bodies of chicken eaters. Prepared by the steps of raw material grinding, extraction, and extraction solution concentration, the Chinese medicinal composition of the invention not only has stable product quality, but also has the advantages of simple process, easily controllable conditions, no discharge of ''three wastes'' during production, and environment friendliness, thus being very suitable for industrial production.
Description
Technical field
The present invention relates to Chinese medicine composition, particularly a kind of Chinese medicine composition for the treatment of infectious bronchitis of chicken and preparation method thereof.
Background technology
Infectious bronchitis of chicken (Infectious bronchitis, IB) be by infectious bronchitis virus (Infectiousbronchitis virus, IBV) cause chicken a kind of acute, the height contagious disease, this disease is in case infect, involve full group rapidly, and being difficult to eradicate, is one of deadly infectious disease of serious harm China poultry husbandry development.At present, IB mainly relies on vaccination to prevent, and strengthens the generation that this disease is avoided in feeding and management simultaneously.Because the serotype of IBV is numerous, the cross immunity level between each strain is low, need vaccine on a large scale, high-intensity use could effectively prevent, the continuous use of the especially weak malicious Seedling of vaccine, cause the variation of IBV easily, cause the formation of highly virulent strain, bring out the generation of IB.Chicken does not have special effect medicine therapeutic after infecting IB, uses the Western medicine symptomatic treatment now more, but effect causes serious drug residue often not as people's will, therefore, seeks new Drug therapy IB and has the important clinical meaning.
Chinese medicine is as a kind of wide, medical material easy, that drug residue is few and toxic and side effects is little of drawing materials of originating, and just there was the record of using herbal mixture treatment disease in China before 2000.In recent years, beam family dependant weighing apparatus (2003), Guo Bing (2009), Li Yanqing (2009) etc. once selected the medical material of effects such as having heat clearing away, detoxifcation, antiviral and enhancing human body immunity power for use, make Chinese medicinal formulae through the science compatibility and treat chicken IB, result of the test shows that therapeutic effect is better.
Therefore, need a kind of Chinese medicine composition for the treatment of infectious bronchitis of chicken, prepare burden simple, with low cost, steady quality, determined curative effect and rapid.
Summary of the invention
One of purpose of the present invention is to provide a kind of Chinese medicine composition and preparation thereof, said composition and can effectively treat infectious bronchitis of chicken by the preparation that said composition is made.
For achieving the above object, technical scheme of the present invention is:
The Chinese medicine composition for the treatment of infectious bronchitis of chicken, Chinese medicine composition is composed of the following components by weight: 8～12 parts in 18～23 parts of Radix Sophorae Tonkinensiss, 12～18 parts of Rhizoma Belamcandae, 12～18 parts of the Rhizoma Anemarrhenaes, 12～18 parts in Gypsum Fibrosum, 12～18 parts in Herba Ephedrae, 8～12 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
The Chinese medicine composition of preferred treatment infectious bronchitis of chicken, composed of the following components by weight: 10 parts in 20 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 15 parts in Gypsum Fibrosum, 15 parts in Herba Ephedrae, 10 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
The Chinese medicine composition of preferred treatment infectious bronchitis of chicken, composed of the following components by weight:: 11 parts in 22 parts of Radix Sophorae Tonkinensiss, 14 parts of Rhizoma Belamcandae, 14 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 11 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
The Chinese medicine composition of preferred treatment infectious bronchitis of chicken, composed of the following components by weight: 12 parts in 18 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 12 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
By the preparation of the Chinese medicine composition of described treatment infectious bronchitis of chicken preparation, described preparation is for carrying immersion or granule.
Two of purpose of the present invention is to provide the preparation method of described preparation, and this method is simple to operate, is applicable to suitability for industrialized production.
For achieving the above object, technical scheme of the present invention is:
The preparation method of the preparation of the Chinese medicine composition preparation for the treatment of infectious bronchitis of chicken, concrete steps are: the described Chinese medicine composition of formula ratio is pulverized the back mix with the water that is equivalent to 8-10 times of weight of Chinese medicine composition and heat lixiviate, the temperature of control lixiviate feed liquid be 75 ℃～80 ℃ and under reflux state lixiviate be no less than 1 hour, separate lixiviating solution and filtering residue; Described lixiviating solution is described preparation.
Further, in described filtering residue, add the water be equivalent to 8-10 times of weight of filtering residue weight and mix and heat lixiviate, the temperature of control lixiviate feed liquid be 75 ℃～80 ℃ and under reflux state lixiviate be no less than 1 hour, separate lixiviating solution and filtering residue; Merge lixiviating solution.
Further, described lixiviating solution is carried out concentrating under reduced pressure, the temperature of control lixiviating solution is between 70 ℃～80 ℃ in the concentration process, and the weight for the treatment of lixiviating solution stops heating and obtains concentrating lixiviating solution after being reduced to 1-2 times of crude drug weight.
Further, adjuvant in concentrating lixiviating solution, and mix homogeneously obtains mixture; The extruding of gained mixture is sieved and is dry, gets granule; Described adjuvant is 25%～40% the dextrin sucrose mixture that is equivalent to concentrate lixiviating solution weight, and the weight ratio of dextrin sucrose mixture sucrose and dextrin is 2.5-4:1.
Further, add 10%～15% the chitosan solution that is equivalent to concentrate lixiviating solution weight in concentrating lixiviating solution, the mass percent of described chitosan solution is 1%.
Radix Sophorae Tonkinensis (Vietnamese Sophora Root) is the dry root and rhizome of Dicotyledoneae leguminous plant Sophora tonkinensis Gagnep..Phytomorph is shrub, high 1～2m.Be born in limestone mountain region or the rock seam.Main product Guangxi.Gather and process autumn and uproot, remove stem and leaf on the ground, dry.Rhizome is irregular nodular pattern, the residual stem foot in top or stem trace, below take root several.The root elongated cylindrical, slightly crooked, long 10～35cm, diameter 0.3～1.5cm; The surface is brown to brownish black, and vertical wrinkle and the hole skin of growing crosswise are arranged.Matter is hard, section skin zone light brown, woody part yellow-white.Little have a bean stink smell, and flavor is extremely bitter.Function cures mainly: the detoxifcation of relieving inflammation or internal heat, reducing swelling and alleviating pain.Be used for throat gingival swelling and pain, cough due to lung-heat's excessive thirst, jaundice, constipation with heat retention.
Rhizoma Belamcandae (Rhizoma Belamcandae) is heat-clearing and detoxifying herb, cures mainly: control the sore throat pharyngalgia, cough with dyspnea, accumulation and obstruction of sputum, scrofula tuberculosis, malaria with splenomegaly, women's amenorrhea, carbuncle sore tumefacting virus.
The Rhizoma Anemarrhenae (Rhizoma Anemarrhenae) cures mainly: epidemic febrile disease, hyperpyrexia excessive thirst, cough and asthma, cough caused by dryness, constipation, osteopyrexia and fever, restlessness of asrhenia type and insomnia, the stranguria with turbid discharge of quenching one's thirst for gunpowder under the heat clearing away.
Gypsum Fibrosum (Gypsum) is main to contain wherein CaO 32.5% of hydrous calcium sulfate (CaSO42H2O), and SO3 46.6%, and H2O 20.9%; Often accompany Organic substance, sulfide etc., and contain elements such as small amount of aluminum, silicon, magnesium, ferrum and micro strontium, barium.
Herba Ephedrae (Herbal Ephedrae) is the wind-cold-dispersing medicinal in HANYAO or the title Chinese medicine; Ancient times is called imperial sand, inferior phase.Include the plant of three kinds of Ephedras: Ephedra sinica (Ephedra sinica), ephedra equisetina (Ephedra equisetina) and epheday intermedia (Ephedra intermedia), the employing position is herb stem.The Ephedra sinica Ephedra sinica is undershrub, often is the draft shape, and stem is high 20 ~ 40 centimetres.Branch is less, and woody stems is short and small, the shape of crawling; The sprig circle, to living or verticillate, long 2.5 ~ 6 centimetres of internode, about 2 millimeters of diameter.
Semen Armeniacae Amarum (Almond; Sweet Almond) latin name: Amygdalus Communis Vas, section's name: Rosaceae (Rosaceae) distributes: originate in the Central Asia, West Asia, Mediterranean Region are introduced a fine variety in the area, warm temperate zone.Semen Armeniacae Amarum is really long on short distance (short spur), has immature outward appearance.When fruit maturation, the shell of its green can split, and manifests the kernel that wraps in the coarse shell, and kernel is for yellow and a lot of duck eyes, shell are arranged is hard wooden.Semen Armeniacae Amarum really is flat avette, end circle, and other end point is covered with the thin skin of brown.Kernel contains 20% protein, and is not starch-containing, and after grinding, pressurizeing, the oils and fats of squeezing out approximately is half of own weight, and almond oil is faint yellow, though there is not fragrance, has the effect of softening skin.
Radix Glycyrrhizae (Radix Glycyrrhizae) is a kind of tonification Chinese herbal medicine.Medicinal part is root and rhizome, and medical material character root is cylindrical, long 25～lOOcm, diameter 0.6～3.5cm.The crust degree of tightness differs, the brown or taupe brown of surface red.Rhizome is cylindrical, and there is the bud trace on the surface, and there is marrow at the section middle part.Feeble QI, it is sweet and special to distinguish the flavor of.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc.Happiness sunlight is abundant, the low dry climate of long temperature at sunshine.Radix Glycyrrhizae is grown in arid, semiarid desert steppe, desert edge and loess hill area more.
The present invention filters out best prescription on the basis of the Chinese medicine composition prescription medicine efficacy screening for the treatment of infectious bronchitis of chicken, prescription is simple, and drug effect is remarkable, has no side effect and drug residue, uses safe and environmentally friendly.
As further improvement, the present invention has further proposed the Chinese medicine composition of the treatment infectious bronchitis of chicken of granule dosage form, reason is that the Chinese medicine composition of great majority control infectious bronchitis of chicken still rests on traditional prescription, compatibility, pulverize, sieve and make the rough stage of powder or powder, and powder and powder to water after easily precipitation and caking, sick chicken usually can't be taken in the effective dose of medicine thing, cause curative effect of medication to be difficult for performance fully, and water preparation no matter, powder or powder all are unfavorable for the formulation of product standard and the evaluation of drug effect, thereby make product quality restive, further influencing its industrialization produces and applies, so characteristics that the present invention drinks in conjunction with physiological structure and the chicken hair dyeing happiness after being ill of chicken, the effective ingredient of described prescription is refined into granule, by the diversion administration, be beneficial to the chicken bulk absorption, curative effect is rapider, not only effectively treat infectious bronchitis of chicken, and make the formulation of product standard and the evaluation of drug effect become possibility, the granule dosage form is beneficial to and stores and transportation simultaneously, is convenient to the promotion and application on a large scale of product when improving safety in utilization.
The preparation method of the Chinese medicine composition for the treatment of infectious bronchitis of chicken of the present invention, by reasonable control operations index, ensured that effective medicinal ingredient is not destroyed and can be come out by abundant lixiviate in the crude drug, technology is simple simultaneously, condition is easily controlled, three-waste free discharge in the production process, environmentally friendly, the Chinese medicine composition of the treatment infectious bronchitis of chicken that makes by the inventive method, steady quality, evident in efficacy reliable, this prescription reduces by 6.9%～24% than MAXINGSHIGAN TANG matched group mortality rate, cure rate improves 6%～26.7%, and effective percentage improves 6.9%～24%; Reduce by 18.2%～35.3% than the mortality rate that infects matched group.Judge by the feature pathological changes scoring of trachea and kidney is comprehensive, to chicken infect pathological lesion behind the IBV to improve effect better, determined curative effect not only, and have no side effect and drug residue, avoid when improving culture benefit the Carnis Gallus domesticus eater is caused health harm, help the raiser significantly to increase economic efficiency, preparation method of the present invention is suitable for industrialization production very much.
The specific embodiment
Preparation method of the present invention also adds chitosan in concentrated lixiviating solution temperature-fall period, chitosan can be removed many unsettled microgranules such as protein, manganese liquid matter, natural gum, saddle matter etc., use the lixiviating solution after concentrating to keep effective ingredient, safety non-toxic largely.
Other advantages of the present invention, target and feature will be set forth to a certain extent in the following description, and to a certain extent, based on being apparent to those skilled in the art to investigating hereinafter, perhaps can obtain instruction from the practice of the present invention.
Embodiment one
The preparation of the Chinese medicinal composition preparation of 1 treatment infectious bronchitis of chicken
(1) take by weighing the Chinese medicine composition 10Kg that treats infectious bronchitis of chicken, above-mentioned 10 Kg Chinese medicine compositions are composed of the following components by weight: 20 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 15 parts in Gypsum Fibrosum, 15 parts in Herba Ephedrae, 10 parts and 10 portions Radix Glycyrrhizaes of Semen Armeniacae Amarum; Chinese medicine composition is pulverized the back pack in the Chinese medicine extraction equipment by the Steam Heating lixiviate with 80kg water, the temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 75-77 ℃;
(2) according to the described method of step (1) medicinal residues that filter gained are carried out 2 lixiviates again, and obtain the lixiviating solution that twice lixiviate produces respectively;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure by steam in the Chinese medicine extraction equipment, the temperature of lixiviating solution control is at 70-72 ℃ in the concentration process, stops when treating the lixiviating solution weight reduction to 10kg concentrating and obtains concentrating lixiviating solution;
When (4) lixiviating solution temperature to be concentrated is down to 60 ℃, to concentrate add in the lixiviating solution dense quality degree be 1% chitosan solution and stir after left standstill 24 hours, filter the precipitation of removing in the solution then, obtain filtrate, wherein, the weight of chitosan solution is for concentrating 15% of lixiviating solution weight.
The preparation method of the Chinese medicine composition of the treatment infectious bronchitis of chicken of present embodiment also comprises the steps:
A. the filtrate of step (4) gained is carried out concentrating under reduced pressure, treat that described filtrate weight adds an amount of dextrin and sucrose and mix homogeneously when being concentrated to 2.6kg and obtains mixture, the weight ratio of described dextrin and sucrose is 4:1.
B. step a gained mixture is pushed 12 mesh sieves and dry under 65 ℃ condition, obtained the Chinese medicine composition product of granule dosage form at last.
The zoopery of the Chinese medicinal composition preparation of 2 treatment infectious bronchitis of chickens (IB)
394 healthy chickens of 2 ages in days are divided into four groups, group I is test group, be specially: add the Chinese medicine composition that makes in the present embodiment after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours in test group drinking-water or feedstuff, the Chinese medicine composition that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; The group II is the counteracting toxic substances matched group, is specially to use the normal feedstuff identical with the normal healthy controls group to feed not administration behind the counteracting toxic substances; The group III is the medicine matched group, be specially after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours add MAXINGSHIGANGSAN (by two 2010 editions preparations of People's Republic of China's veterinary drug allusion quotation) in test group drinking-water or feedstuff, it is suitable that the MAXINGSHIGANGSAN that every day, every chicken was joined and 1.0 restrains the raw material of Chinese medicine doses; The group IV is the normal healthy controls group, only feeds with normal feedstuff.Wherein, group I, group II and group III are divided into 4 parallel groups separately; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law, observe disease chicken symptom every day when 9:00 and 16:00, dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and perform itemized record, carry out dead chicken being weighed, dissecting in the 9th day to test, and record pathological changes situation, after test is finished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data sees Table-1.
Table-1 Chinese medicinal formulae is to the therapeutic effect of IB
Annotate: the complete different persons of lower case be significant difference (
P＜0.05)
Data explanation from table: the Chinese medicine composition of the treatment infectious bronchitis of chicken that present embodiment makes can be treated infectious bronchitis of chicken effectively, effective percentage is 76.2%, compare with the counteracting toxic substances matched group, reduce disease chicken death rate 23.9%,, relatively reduce disease chicken death rate 24.0% with the medicine matched group.
Embodiment two
The preparation of the Chinese medicinal composition preparation of 1 treatment infectious bronchitis of chicken
(1) take by weighing the Chinese medicine composition 10Kg that treats infectious bronchitis of chicken, above-mentioned 10 Kg Chinese medicine compositions are composed of the following components by weight: 22 parts of Radix Sophorae Tonkinensiss, 14 parts of Rhizoma Belamcandae, 14 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 11 parts and 11 portions Radix Glycyrrhizaes of Semen Armeniacae Amarum; To pack in the Chinese medicine extraction equipment by the Steam Heating lixiviate with 90kg water after the above-mentioned pulverizing medicinal materials, the temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 77-80 ℃;
(2) according to the described method of step (1) medicinal residues that filter gained are carried out 2 lixiviates again, and obtain the lixiviating solution that twice lixiviate produces respectively;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure by steam in the Chinese medicine extraction equipment, the temperature of lixiviating solution control is at 78～80 ℃ in the concentration process, treats that the lixiviating solution weight reduction is to stop behind the 20kg concentrating and obtain concentrating lixiviating solution;
When (4) lixiviating solution temperature to be concentrated is down to 50 ℃, to concentrate add in the lixiviating solution mass concentration be 1% chitosan solution and stir after left standstill 24 hours, filter the precipitation of removing in the solution then, obtain filtrate, wherein, the weight of chitosan solution is for concentrating 10% of lixiviating solution weight.
The preparation method of the Chinese medicine composition of the treatment infectious bronchitis of chicken of present embodiment also comprises the steps:
A. the filtrate of step (4) gained is carried out concentrating under reduced pressure, the weight for the treatment of described filtrate adds an amount of dextrin and sucrose and mix homogeneously when being concentrated to 4kg and obtains mixture, and the weight ratio of described dextrin and sucrose is 2.5:1.
B. step a gained mixture is pushed 12 mesh sieves and dry under 60 ℃ condition, obtained the Chinese medicine composition product of granule dosage form at last.
Certainly, among the present embodiment step a, it is that 25-40% of crude drug weight all can realize purpose of the present invention that filtrate decompression is concentrated into weight, and the weight ratio of dextrin and sucrose is processing and the molding that 2.5-4:1 all can ensure granule in the subsequent technique.
The zoopery of the Chinese medicinal composition preparation of 2 treatment infectious bronchitis of chickens
394 healthy chickens of 2 ages in days are divided into four groups, group I is test group, be specially: add the Chinese medicine composition that makes in the present embodiment after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours in test group drinking-water or feedstuff, the Chinese medicine composition that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; The group II is the counteracting toxic substances matched group, is specially to use the normal feedstuff identical with the normal healthy controls group to feed not administration behind the counteracting toxic substances; The group III is the medicine matched group, is specially after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours to add MAXINGSHIGANGSAN in test group drinking-water or feedstuff, and the MAXINGSHIGANGSAN that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; The group IV is the normal healthy controls group, only feeds with normal feedstuff.Wherein, group I, group II and group III are divided into 4 parallel groups separately; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law, observe disease chicken symptom every day when 9:00 and 16:00, dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and perform itemized record, carry out dead chicken being weighed, dissecting in the 9th day to test, and record pathological changes situation, after test is finished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data sees Table-2.
Table-2 Chinese medicinal formulaes are to the therapeutic effect of IB
Annotate: the complete different persons of lower case be significant difference (
P＜0.05)
Data explanation from table: the Chinese medicine composition of the treatment infectious bronchitis of chicken that present embodiment makes can be treated infectious bronchitis of chicken effectively, effective percentage is 75.0%, compare with the counteracting toxic substances matched group, reduce disease chicken death rate 24.9%,, relatively reduce disease chicken death rate 18.5% with the medicine matched group.
Embodiment three
The preparation of the Chinese medicinal composition preparation of 1 treatment infectious bronchitis of chicken
(1) take by weighing the Chinese medicine composition 10Kg that treats infectious bronchitis of chicken, above-mentioned 10 Kg Chinese medicine compositions are composed of the following components by weight: 18 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 12 parts and 12 portions Radix Glycyrrhizaes of Semen Armeniacae Amarum; To pack in the Chinese medicine extraction equipment by the Steam Heating lixiviate with 80kg water after the above-mentioned pulverizing medicinal materials, the temperature that control is heated material is filtered material behind the backflow 1h and is obtained lixiviating solution and medicinal residues between 70-72 ℃;
(2) according to the described method of step (1) medicinal residues that filter gained are carried out 3 lixiviates again, and obtain the lixiviating solution that three lixiviates produce respectively;
(3) pack into after the lixiviating solution of step (1) and step (2) gained is merged and carry out concentrating under reduced pressure by steam in the Chinese medicine extraction equipment, the temperature of lixiviating solution control is at 74～76 ℃ in the concentration process, treats that the lixiviating solution weight reduction is to stop behind the 10kg concentrating and obtain concentrating lixiviating solution;
When (4) lixiviating solution temperature to be concentrated is down to 60 ℃, to concentrate add in the lixiviating solution dense quality degree be 1% chitosan solution and stir after left standstill 24 hours, filter the precipitation of removing in the solution then, obtain filtrate, wherein, the weight of chitosan solution is for concentrating 15% of lixiviating solution weight.
The preparation method of the Chinese medicine composition of the treatment infectious bronchitis of chicken of present embodiment also comprises the steps:
A. the filtrate of step (4) gained is carried out concentrating under reduced pressure, treat that described filtrate weight adds an amount of dextrin and sucrose and mix homogeneously when being concentrated to 2.5kg and obtains mixture, the weight ratio of described dextrin and sucrose is 4:1.
B. step a gained mixture is pushed 12 mesh sieves and dry under 65 ℃ condition, obtained the Chinese medicine composition product of granule dosage form at last.
The zoopery of the Chinese medicinal composition preparation of 2 treatment infectious bronchitis of chickens
394 healthy chickens of 2 ages in days are divided into four groups, group I is test group, be specially: add the Chinese medicine composition that makes in the present embodiment after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours in test group drinking-water or feedstuff, the Chinese medicine composition that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; The group II is the counteracting toxic substances matched group, is specially to use the normal feedstuff identical with the normal healthy controls group to feed not administration behind the counteracting toxic substances; The group III is the medicine matched group, is specially after counteracting toxic substances the infectious bronchitis typical clinical symptom occurred in 48 hours to add MAXINGSHIGANGSAN in test group drinking-water or feedstuff, and the MAXINGSHIGANGSAN that every day, every chicken was joined is suitable with 1.0 gram raw material of Chinese medicine doses; The group IV is the normal healthy controls group, only feeds with normal feedstuff.Wherein, group I, group II and group III are divided into 4 parallel groups separately; Each organizes chicken free choice feeding, drinking-water, Routine Management; Raise identical natural law, observe disease chicken symptom every day when 9:00 and 16:00, dead chicken is weighed, anatomic observation fabricius bursa pathology changes, and perform itemized record, carry out dead chicken being weighed, dissecting in the 9th day to test, and record pathological changes situation, after test is finished, test mortality rate, cure rate, effective percentage are carried out data statistics.Relevant data sees Table-3.
Table-3 Chinese medicinal formulaes are to the therapeutic effect of IB
Annotate: the complete different persons of lower case be significant difference (
P＜0.05)
Data explanation from table: the Chinese medicine composition of the treatment infectious bronchitis of chicken that present embodiment makes can be treated infectious bronchitis of chicken effectively, effective percentage is 75.0%, compare with the counteracting toxic substances matched group, reduce disease chicken death rate 26.7%,, relatively reduce disease chicken death rate 20.7% with the medicine matched group.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although by invention has been described with reference to the preferred embodiments of the present invention, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and not depart from the spirit and scope of the present invention that appended claims limits.
Claims (10)
1.. the Chinese medicine composition for the treatment of infectious bronchitis of chicken, it is characterized in that: Chinese medicine composition is composed of the following components by weight: 8～12 parts in 18～23 parts of Radix Sophorae Tonkinensiss, 12～18 parts of Rhizoma Belamcandae, 12～18 parts of the Rhizoma Anemarrhenaes, 12～18 parts in Gypsum Fibrosum, 12～18 parts in Herba Ephedrae, 8～12 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
2.. the Chinese medicine composition for the treatment of infectious bronchitis of chicken according to claim 1 is characterized in that: Chinese medicine composition is composed of the following components by weight: 10 parts in 20 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 15 parts in Gypsum Fibrosum, 15 parts in Herba Ephedrae, 10 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
3. the Chinese medicine composition for the treatment of infectious bronchitis of chicken according to claim 1, it is characterized in that: Chinese medicine composition is composed of the following components by weight: 11 parts in 22 parts of Radix Sophorae Tonkinensiss, 14 parts of Rhizoma Belamcandae, 14 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 11 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
4. the Chinese medicine composition for the treatment of infectious bronchitis of chicken according to claim 1, it is characterized in that: Chinese medicine composition is composed of the following components by weight: 12 parts in 18 parts of Radix Sophorae Tonkinensiss, 15 parts of Rhizoma Belamcandae, 15 parts of the Rhizoma Anemarrhenaes, 14 parts in Gypsum Fibrosum, 14 parts in Herba Ephedrae, 12 parts in Semen Armeniacae Amarum and Radix Glycyrrhizae.
5. by the preparation of the Chinese medicine composition of the described treatment infectious bronchitis of chicken of claim 1 preparation, it is characterized in that: described preparation is lixiviating solution or granule.
6. the preparation method of the described preparation of claim 5, it is characterized in that, concrete steps are: the described Chinese medicine composition of formula ratio is pulverized the back mix with the water that is equivalent to 8-10 times of weight of Chinese medicine composition and heat lixiviate, the temperature of control lixiviate feed liquid be 75 ℃～80 ℃ and under reflux state lixiviate be no less than 1 hour, separate lixiviating solution and filtering residue; Described lixiviating solution is described preparation.
7. the preparation method of preparation according to claim 6, it is characterized in that, in described filtering residue, add the water mixing that is equivalent to 8-10 times of weight of filtering residue weight and heat lixiviate, the temperature of control lixiviate feed liquid be 75 ℃～80 ℃ and under reflux state lixiviate be no less than 1 hour, separate lixiviating solution and filtering residue; Merge lixiviating solution.
8. according to the preparation method of claim 6 or 7 described preparations, it is characterized in that, described lixiviating solution is carried out concentrating under reduced pressure, the temperature of control lixiviating solution is between 70 ℃～80 ℃ in the concentration process, and the weight for the treatment of lixiviating solution stops heating and obtains concentrating lixiviating solution after being reduced to 1-2 times of crude drug weight.
9. according to the preparation method of claim 6 or 7 described preparations, it is characterized in that, in concentrating lixiviating solution, add adjuvant, and mix homogeneously obtains mixture; The extruding of gained mixture is sieved and is dry, gets granule; Described adjuvant is 25%～40% the dextrin sucrose mixture that is equivalent to concentrate lixiviating solution weight, and the weight ratio of dextrin sucrose mixture sucrose and dextrin is 2.5-4:1.
10. the preparation method of preparation according to claim 9 is characterized in that, adds 10%～15% the chitosan solution that is equivalent to concentrate lixiviating solution weight in concentrating lixiviating solution, and the mass percent of described chitosan solution is 1%.
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| CN101757308A (en) * | 2008-10-31 | 2010-06-30 | 苏州科牧动物药品有限公司 | Healthcare medicine for preventing and treating poultry respiratory symptoms |
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