CN102488724A - Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof - Google Patents
Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof Download PDFInfo
- Publication number
- CN102488724A CN102488724A CN2011104329654A CN201110432965A CN102488724A CN 102488724 A CN102488724 A CN 102488724A CN 2011104329654 A CN2011104329654 A CN 2011104329654A CN 201110432965 A CN201110432965 A CN 201110432965A CN 102488724 A CN102488724 A CN 102488724A
- Authority
- CN
- China
- Prior art keywords
- extract
- ethyl acetate
- drop pill
- glad
- brain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and a preparation method thereof. The preparation method comprises the following steps of: (1) weighing raw materials: Chinese angelica and szechuan lovage rhizome; (2) adding the raw materials into an ethanol aqueous solution, heating, reflowing, extracting, filtering, recovering ethanol from an extracting solution, concentrating to obtain thick paste, cooling to the room temperature, extracting with ethyl acetate for 2-5 times, combining extract liquors, and recovering ethyl acetate from the extract liquor till the smell of ethyl acetate disappears to obtain an extract; and (3) heating at least one of polyethylene glycol-4000 and polyethylene glycol-6000 in a water bath for melting, adding the extract, fully mixing uniformly, and dripping to obtain the dripping pills. The dripping pills have low cost, quick response and a good curative effect, and are safe. The method disclosed by the invention has the advantages of small quantity of labor hours, high transfer rate of effective components, higher safety for operating personnel and suitability for industrial mass production; and particularly, the ethyl acetate is taken as an extracting agent in an extracting step, so that total production cost is lowered greatly.
Description
Technical field
The invention belongs to the Chinese medicine preparation field, relate to a kind of medicine of treating cardiovascular and cerebrovascular disease and preparation method thereof.
Background technology
Utilize the pharmaceutical composition of Chinese herbal treatment cardiovascular and cerebrovascular disease a lot, process decoction or process honeyed pill, the watered pill but adopt method that traditional decoction endures to extract medicine more, patient's taking dose is big, and onset is slower, and relative cost is high, and patient's compliance is poor.
The former technology of producing the glad drop pill of brain that relaxes has overcome above-mentioned deficiency, and percolation is adopted in the extraction of raw material of Chinese medicine, and active ingredient is concentrated, and effective ingredient improves relatively, and patient's taking dose reduces, but this method production cycle is longer, and efficient is lower; Particularly extraction step adopts ether as extractant; The ether boiling point is low, volatile, flash-point is low, have character such as narcoticness, has certain danger, and operating environment is had relatively high expectations; Especially under the temperature condition with higher, there is bigger safety hidden danger when producing greatly.
Summary of the invention
The objective of the invention is to overcome the deficiency of prior art, provide a kind of and be suitable for that industry big is produced, cost is low, safety is rapid-action, the glad drop pill of easypro brain of eutherapeutic treatment cardiovascular and cerebrovascular disease.
Second purpose of the present invention provides a kind of method for preparing of treating the glad drop pill of easypro brain of cardiovascular and cerebrovascular disease.
Technical scheme of the present invention is summarized as follows:
The method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease comprises the steps:
(1) be that the ratio of 1-8: 1-8 takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) the raw material adding being equivalent to raw material 5-9 quality concentration expressed in percentage by volume doubly is the ethanol water of 65%-90%, and heating and refluxing extraction is filtered; Filtering residue adds said ethanol water again, and heating and refluxing extraction is extracted 1-4 time altogether; The each extraction 1-3 hour reclaimed extracting solution ethanol and is concentrated into that relative density is 1.00～1.30 70-90 ℃ the time, thick paste; Be cooled to room temperature,, extract 2-5 time with quite said thick paste quality 2-4 ethyl acetate extraction doubly; Combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
(3) will be equivalent to said extract quality 2-7 Polyethylene Glycol-4000 doubly and Polyethylene Glycol-6000 at least a in water-bath heating and melting, add said extract, fully mix the back and droplet process drop pill.
(4) bag film-coat.
The mass ratio of said Radix Angelicae Sinensis and Rhizoma Chuanxiong is preferably 1: 1.
Said step (2) is: raw material is added to be equivalent to raw material 5 quality concentration expressed in percentage by volume doubly be 85% ethanol water, heating and refluxing extraction is filtered; Filtering residue adds said ethanol water again, and heating and refluxing extraction is extracted 2 times altogether; The each extraction 2 hours reclaimed extracting solution ethanol and is concentrated into that relative density is 1.10-1.15 85 ℃ the time, thick paste; Be cooled to room temperature,, extract 3 times with quite said thick paste quality 2-4 ethyl acetate extraction doubly; Combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract.
Said step (3) is: will be equivalent to Polyethylene Glycol-6000 heating and melting in water-bath of 3 times of said extract qualities, and add said extract, and drip after fully mixing and process drop pill.
The drop pill of method for preparing.
Advantage of the present invention is:
The glad drop pill cost of the easypro brain of treatment cardiovascular and cerebrovascular disease of the present invention is low, safety is rapid-action, good effect.
Method of the present invention is few its man-hour, and the effective ingredient rate of transform is high, particularly the extractant that adopts of extraction step be ethyl acetate it the whole production cost is reduced greatly, safety of operators is improved, be suitable for that industry is big produces.
Description of drawings
The antagonism that the rat aortic article that Fig. 1 causes KCL for the glad drop pill of brain that relaxes shrinks.Wherein:
The present invention: y=31.777x+152.97R=1
Former technology: y=17.03x+94.143R=0.9995
The specific embodiment
Below embodiment be in order to enable those skilled in the art to understand better the present invention, but the present invention is not done any restriction.
The method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease comprises the steps:
(1) be that 1: 1 ratio takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) raw material is added to be equivalent to raw material 6 quality concentration expressed in percentage by volume doubly be 85% ethanol water, heating and refluxing extraction is filtered, and filtering residue adds said ethanol water again; Extract altogether 2 times, extracted 2 hours at every turn, extracting solution is reclaimed ethanol and is concentrated into that relative density is 1.10 85 ℃ the time; Get thick paste, be cooled to room temperature, with the ethyl acetate extraction of 3 times of quite said thick paste quality; Extract 3 times, combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
(3) will be equivalent to Polyethylene Glycol-6000 heating and melting in water-bath of 3 times of the quality of said extract, add said extract, and fully mix the back and drip and process drop pill.
(4) with Opadry bag film-coat.
Embodiment 2
The method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease comprises the steps:
(1) be that 1: 8 ratio takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) raw material is added to be equivalent to raw material 5 quality concentration expressed in percentage by volume doubly be 90% ethanol water, heating and refluxing extraction is filtered, and filtering residue adds said ethanol water again; Extract altogether 2 times, extracted 2 hours at every turn, extracting solution is reclaimed ethanol and is concentrated into that relative density is 1.00 90 ℃ the time; Get thick paste, be cooled to room temperature, with the ethyl acetate extraction of 2 times of quite said thick paste quality; Extract 5 times, combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
(3) will be equivalent to Polyethylene Glycol-4000 heating and melting in water-bath of 2 times of the quality of said extract, add said extract, and fully mix the back and drip and process drop pill.
Embodiment 3
The method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease comprises the steps:
(1) be that 8: 1 ratio takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) raw material is added to be equivalent to raw material 7 quality concentration expressed in percentage by volume doubly be 65% ethanol water, heating and refluxing extraction is filtered, and filtering residue adds said ethanol water again; Extract 4 times, extracted 1 hour at every turn, extracting solution is reclaimed ethanol and is concentrated into that relative density is 1.30 80 ℃ the time; Get thick paste, be cooled to room temperature, with the ethyl acetate extraction of 4 times of quite said thick paste quality; Extract 2 times, combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
The mass ratio that (3) will be equivalent to 5 times of the quality of said extract is 1: 1 Polyethylene Glycol-4000 and Polyethylene Glycol-6000 heating and melting in water-bath, adds said extract, fully mixes the back and drips and process drop pill;
(4) with Opadry bag film-coat.
Embodiment 4
The method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease comprises the steps:
(2) be that 5: 3 ratio takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) raw material is added to be equivalent to raw material 9 quality concentration expressed in percentage by volume doubly be 80% ethanol water, heating and refluxing extraction 1 time, 3 hours; Filter, extracting solution reclaimed ethanol and is concentrated into that relative density is 1.30 70 ℃ the time, thick paste; Be cooled to room temperature, the ethyl acetate extraction with 3 times of quite said thick paste quality extracts 3 times; Combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
The mass ratio that (3) will be equivalent to 7 times of the quality of said extract is 1: 1 Polyethylene Glycol-4000 and Polyethylene Glycol-6000 heating and melting in water-bath, adds said extract, fully mixes the back and drips and process drop pill.
Method of the present invention and former technology compare: former process using percolation extracts raw material, and the production cycle is longer, and efficient is lower; Adopt ether as solvent in the extraction process; Its boiling point is low, volatile, flash-point is low, have certain character such as narcoticness, has certain danger, and operating environment is had relatively high expectations; Especially under the temperature condition with higher, there is bigger safety hidden danger when producing greatly.Production technology of the present invention rationally, science more.
Data: (table 1)
The present invention compares with former technology, and method of the present invention is extracted and significantly reduced man-hour, and the employing ethyl acetate is an extractant, and the effective ingredient rate of transform increases, and safety improves, and cost reduces greatly.
Experiment
Experiment material
1. receive reagent:
The glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease of the present invention (be called for short: new technology), the sepia decorating film, every g is equivalent to the 5g crude drug, embodiment 1 preparation.The glad drop pill of easypro brain of former technology, the sepia decorating film, every g is equivalent to the 5g crude drug, lot number: 090503, provide by No.6 Chinese Medicine Factory, Tianjin Zhongxin Pharmaceutical Industry Group Co.Shady and cool dry place preserves, and faces with preceding adding distil water dilution, is mixed with debita spissitudo, supplies animal ig administration.
Test dose: according to the clinical using dosage 5.04g of former technology crude drug/people/sky, this EXPERIMENTAL DESIGN dosage does
Rat: 0.4g crude drug/kg (high dose), 0.2g crude drug/kg (middle dosage), 0.1g crude drug/kg (low dosage)
Mice: 0.8g crude drug/kg (high dose), 0.4g crude drug/kg (middle dosage), 0.2g crude drug/kg (low dosage)
2. contrast medicine:
2.1 aspirin, AstraZeneca pharmaceutical Co. Ltd produces, lot number: 0708033.
2.2 diazepam: Tianjin gold credit aminoacid company limited, lot number 0510241
3. tool drugs and instrument:
3.1 glacial acetic acid: Tianjin Milky Way chemical reagent factory produces, lot number: 20050609.Face with preceding and become 0.67% concentration with distilled water diluting.
3.2 sodica calx (A type): Shanghai the May 4th chemical reagent company limited, lot number: 090207.
3.3 dextran T500, the biological import packing of couple stars, lot number 17-0320-02.
3.4 normal saline: Jin Lan pharmaceutcal corporation, Ltd in Tianjin produces, lot number 08101101.
3.5 pentobarbital sodium: the import packing of Beijing chemical reagents corporation Germany, lot number 060222.
3.6 heparin calcium inj: Tianjin Biochemical Pharmaceutical Factory, lot number 20090301.
3.7NaCL: Tianjin Milky Way chemical reagent factory, lot number: 20050713.
3.8KCL: Tianjin recovery fine chemistry industry institute, lot number: 20051119.
3.9CaCL
2: Tianjin Milky Way chemical reagent factory, lot number: 20040310.
3.10KH2PO
4: Tianjin Milky Way chemical reagent factory, lot number: 20050321.
3.11MgSO
4: Tianjin Milky Way chemical reagent factory, lot number: 20060403.
3.12NaHCO
3: Tianjin Milky Way chemical reagent factory, lot number: 20030922.
3.13GLU: Tianjin chemical reagent three factories, lot number: 20050908.
3.14Kerbs-Henseleit liquid: self-control; Compound method: take by weighing NaCL6.92g, KCL0.35g, CaCL
20.28g, KH2PO
40.16g, MgSO
40.16g, NaHCO
32.1g GLU 2.0g adds distilled water and is mixed with 1000ml solution.
Industrial scientific & trading Co., Ltd. produces 3.15JZ101 type tonotransducer, Beijing newly navigate.
3.16MP-150 lead the physiological signal monitor, BIOPAC company produces more.
3.17HSS-1B the type thermostatic bath, Chengdu Instruement Factory.
3.18JZZ98-CM the independent activity of animals appearance, institute of Materia Medica,Chinese Academy of Medical Sciences.
3.19LXJ-IIB type low speed high capacity multitube centrifuge, last Hai'an booth scientific instrument company limited.
3.20PL203 type electronic balance: prunus mume (sieb.) sieb.et zucc. Teller-Tuo benefit Instr Ltd..
3.21T2000 electronic balance: two outstanding brother's (group) company limiteies of the U.S..
3.22BT87-3 the type thrombus in vivo forms analyzer, medical college cardiovascular research chamber, packet header.
3.23LG-R-80C blood viscosity instrument, diligent generation Supreme Being's scientific instrument company in Beijing.
Laboratory animal
ICR kind mice, the SD rat, the SPF level is purchased in Beijing Vital River Experimental Animals Technology Co., Ltd. and Tianyin Test Animal Centre.Raising is having in the observation ward of central air-conditioning (accurate No. 012 of the real facility in Tianjin meets the barrier grade standard), and the special-purpose full nutrition piece of edible Mus material (Tianjin China flourish laboratory animal Science and Technology Ltd. produces) drinks pure water.
Test method and result
One. analgesic activity
1. the influence of the mice acetic acid twisting being reacted
60 ICR kinds of experiment selected mice, body weight 21-24g is divided into 6 groups at random, and 10 every group, male and female half and half.The glad drop pill group of brain of relaxing is pressed ig every day of dosage shown in the table 2 administration 1 time, successive administration 3 days.Positive drug control group is in test ig administration on the same day 1 time, and matched group gives with the volume distilled water.Each organizes mice after the last administration 40 minutes, and the glacial acetic acid solution 0.2ml/ of lumbar injection 0.67% only turns round the body number of times with what the counter records glacial acetic acid caused bitterly back second every mice in 10 minutes.Get on average turn round body number and the matched group of each administration group and on average turn round the body number relatively, result's (seeing table 2) shows, but the high, medium and low dosage of the glad drop pill of easypro brain of new technology all highly significant ground minimizing acetic acid induced mice turn round the body number of times.Show that pain that this medicine causes chemical stimulation has the analgesic activity of highly significant, and its action intensity is superior to former technology group.
The easypro glad drop pill of brain of table 2 is to the influence
of mice acetic acid twisting reaction
2. the influence of mice water-bath whipping being reacted
60 ICR kind mices that the experiment selected threshold value is qualified, body weight 18-22g is divided into 6 groups at random, and 10 every group, male and female half and half.Relaxing, the glad drop pill of brain is former, each group of new technology is pressed ig every day of dosage shown in the table 3 administration 1 time, successive administration 3 days.Positive drug control group is in test ig administration on the same day 1 time, and matched group gives with the volume distilled water.0.5h, 1h, 2h immerse mouse tail point 1.5cm in the water bath with thermostatic control (55 ± 0.3 ℃) respectively before test and after the last administration, write down the Mus tail Zi immersing in the water to the time that the whipping action occurs, as the incubation period of whipping reaction with the Casio stopwatch.The average TFL and the average TFL of matched group of each administration group are compared, and result's (seeing table 3) shows, but 0.5 hour, the 1 hour equal significant prolongation mice whipping response time behind each dose groups medicine of the glad drop pill of easypro brain of new technology.Show that the pain that the glad drop pill of easypro brain of new technology causes warm stimulation has significant analgesic activity, its analgesic activity intensity is not less than former technology group.
The easypro glad drop pill of brain of table 3 is to the influence (
n=10) of mice water-bath whipping reaction
Two. function of promoting blood circulation to disperse blood clots
1. the influence that the rat thrombus in vivo is formed
50 SD rats of experiment selected, body weight 200-220g is divided into 5 groups at random by body weight, and 10 every group, male and female half and half, each group of new, the former technology of the glad drop pill of brain of relaxing is pressed table 4 ig every day administration 1 time, and matched group gives with the volume distilled water, successive administration 7 days.1h after the last administration, lumbar injection 45mg/kg pentobarbital sodium anesthetized animal, it is fixing that rat faces upward the position, separates right carotid.The stimulating electrode that the experimental thrombus in vivo of BT87-3 type is formed appearance places the common carotid artery proximal part, and temperature probe places distal end, and stimulating current intensity is 2mA.Stimulate after 5 minutes, close stimulating electrode, after 3 minutes, regulate temp controlled meter to zero-bit, record begins to the required time of tremulous pulse temperature decrease (s) from stimulation, that is the artery thrombosis time.Result's (seeing table 4) shows that each dose groups of the glad drop pill of easypro brain of new technology all can prolong the artery thrombosis time in highly significant ground.Show that this medicine can resist the formation of arterial thrombus, the function of promoting blood circulation to disperse blood clots of highly significant is arranged, its function of promoting blood circulation to disperse blood clots is superior to former technology group.
Table 4 relaxes the glad drop pill of brain to thrombotic influence in the rat experiment gonosome
2. to the influence of blood stasis model hemorheology of rat
60 SD rats of experiment selected, body weight 200-220g is divided into 6 groups at random, and 10 every group, male and female half and half.Each group is pressed ig every day of dosage shown in the table 5 administration 1 time, and model group and matched group give the equal-volume distilled water, successive administration 7 days.After the last administration 30 minutes, except that matched group, all the other were respectively organized rat tail vein and inject 10% macromolecule right rotary glycoside solution 0.4ml/100g.After 30 minutes, the pentobarbital sodium anesthetized animal, abdominal aortic blood is put into the centrifuge tube that contains heparin sodium, shakes up, and gets the 0.8ml whole blood, inserts in the LG-R-80C type blood viscosity instrument, and measuring and cutting speed is 200S
-1, 30S
-1, 5S
-1, 1S
-1The time viscosity.Centrifugal 10 minutes of 2000 rev/mins of blood of residue, separated plasma is measured plasma viscosity.Result's (seeing table 5) shows that behind the model group rat tail vein injection macromolecule right rotary glycoside, whole blood and plasma viscosity are apparently higher than matched group, but and each dosage of the glad drop pill of easypro brain of new technology all reduces WBV and the plasma viscosity of respectively cutting under the speed in highly significant ground.Show that it can significantly improve the hemorheology of blood stasis model rat, have the function of promoting blood circulation to disperse blood clots of highly significant, its effect is similar with the former technology group of the glad drop pill of the brain that relaxes.
The easypro glad drop pill of brain of table 5 is to the influence (
n=10) of blood stasis model hemorheology of rat
Three. spasmolysis (antagonism that the rat aortic article that KCL is caused shrinks)
Select qualified SD rat for use, the male and female dual-purpose, body weight 250-350g, the anesthesia of 20% urethane is got rat aorta and is put into feeding 95%O immediately
2, 5%CO
2In the Kerbs-Henseleit liquid of gaseous mixture, be cut into the spiral bar of 2.5mm * 20mm, spiral bar be fixed in the Magnus' bath that 10mlKerbs-Henseleit liquid is housed, feed 95%O
2, 5%CO
2Gaseous mixture applies preload 1g, and is movable with the MP150 type polygraph record aortic article of BIOPAC company.Aortic article was stablized about 1 hour; Changed liquid 1 time in 20 minutes during this time, treat that aortic article is stable after, add 1mol/L KCL100 μ l; The contraction movement of record aortic article; After treating that the aorta contraction is stable, add the medicinal liquid 100 μ l of the glad drop pill respective concentration of easypro brain of former technology and new technology successively, the activity change of record aortic article.Measure before each drug dose group aortic article administration and the active meansigma methods of administration after-contraction, calculate suppression ratio.
Shrinkage value * 100% before suppression ratio (%)=(shrinkage value before the medicine-medicine after-contraction value)/medicine
Experimental result (seeing table 6) shows, final concentration 4 * 10
-5, 4 * 10
-4, 4 * 10
-3, and 4 * 10
-2The rat aortic article that g crude drug/ml causes KCL shrinks the antagonism of highly significant is arranged, and the better linearity relation is arranged.(see figure 1)
The antagonism (suppression ratio) that the rat aortic article that the easypro glad drop pill of brain of table 6 causes KCL shrinks
Four. sedation
1. to the influence of mice autonomic activities
60 of ICR kind mices are chosen in test, and body weight 20-24g is divided into 6 groups at random, and 10 every group, male and female half and half.Press the easypro glad drop pill ig of the brain administration of dosage shown in the table 7, every day 1 time, for three days on end, positive drug is stable to be organized in test gastric infusion on the same day once.40min after the last administration puts into JZZ98-CM mice autonomic activities appearance active box with animal, behind the adaptation 5min, and mice autonomic activities number of times in the record 5min.Result of the test (seeing table 7) shows that the glad drop pill of the easypro brain of new technology is high, middle dose groups has significant sedation to the mice autonomic activities, shows that it has tangible sedation to mice, and its effect is not less than former technology group.
The easypro glad drop pill of brain of table 7. is to the influence of mice autonomic activities
2. with the synergism of sub-threshold dose pentobarbital sodium
60 of ICR kind mices are chosen in test, and body weight 20-24g is divided into 6 groups at random, and 10 every group, male and female half and half.Press dosed administration shown in the table 8, every day 1 time, for three days on end; 60min after the last administration; The pentobarbital sodium 30mg/kg of lumbar injection sub-threshold dose observes and the number of elements of sleep mice takes place record, and reaching the above person of 1min with righting reflex loss in the 15min behind the injection pentobarbital sodium is sleep.Each significance test of organizing test data adopts X
2Check.Result's (seeing table 8) shows: stable pentobarbital sodium with sub-threshold dose has the synergism of highly significant; Under this test dose; The glad drop pill of easypro brain of new technology and the pentobarbital sodium of sub-threshold dose do not see that synergism is arranged, and the pentobarbital sodium of former technology and sub-threshold dose is not also seen has synergism.
The synergism of easypro glad drop pill of brain of table 8. and pentobarbital sodium
Conclusion (of pressure testing)
The glad drop pill of easypro brain of new technology is under ig0.1g crude drug/kg-0.8g crude drug/kg dosage, but the equal significant prolongation mice whipping response time of each dose groups, and highly significant ground reduces the acetic acid induced mice and turns round the body number of times; Each dose groups all can prolong the artery thrombosis time in highly significant ground, and highly significant ground reduces the blood stasis model rat and respectively cuts WBV and plasma viscosity under the speed; Final concentration 4 * 10
-5, 4 * 10
-4, 4 * 10
-3, and 4 * 10
-2G crude drug/ml shrinks the antagonism that highly significant is arranged to the rat aortic article that KCL causes; High, middle dose groups has significant sedation to the mice autonomic activities, but does not see that with the pentobarbital sodium of sub-threshold dose the obvious synergistic effect is arranged, and former technology is not also seen has synergism.
The above results shows; The glad drop pill of easypro brain of new technology has pain relieving, the blood circulation promoting and blood stasis dispelling of highly significant to reach arterial vascular spasmolysis; And have anoxia enduring and sedation; Show that the easypro glad drop pill drug action of brain of new technology is consistent with former process acts, and action intensity is superior to or be same as former technology approximately.
Above-mentioned result of the test is intended using clinically for the glad drop pill new technology of brain of relaxing provides experimental basis.
Experimental result shows that the glad drop pill of easypro brain of the treatment cardiovascular and cerebrovascular disease of embodiment 2-embodiment 4 preparations is similar with embodiment 1, also has above-mentioned effect.
Claims (6)
1. the method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease is characterized in that comprising the steps:
(1) be that the ratio of 1-8: 1-8 takes by weighing raw material: Radix Angelicae Sinensis and Rhizoma Chuanxiong in mass ratio;
(2) the raw material adding being equivalent to raw material 5-9 quality concentration expressed in percentage by volume doubly is the ethanol water of 65%-90%, and heating and refluxing extraction is filtered; Filtering residue adds said ethanol water again, and heating and refluxing extraction is extracted 1-4 time altogether; The each extraction 1-3 hour reclaimed extracting solution ethanol and is concentrated into that relative density is 1.00～1.30 70-90 ℃ the time, thick paste; Be cooled to room temperature,, extract 2-5 time with quite said thick paste quality 2-4 ethyl acetate extraction doubly; Combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract;
(3) will be equivalent to said extract quality 2-7 Polyethylene Glycol-4000 doubly and Polyethylene Glycol-6000 at least a in water-bath heating and melting, add said extract, fully mix the back and droplet process drop pill.
2. the method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease according to claim 1 is characterized in that also comprising: the bag film-coat.
3. the method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease according to claim 1 and 2, the mass ratio that it is characterized in that said Radix Angelicae Sinensis and Rhizoma Chuanxiong is 1: 1.
4. the method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease according to claim 1 and 2 is characterized in that said step (2) is: raw material is added to be equivalent to raw material 5 quality concentration expressed in percentage by volume doubly be 85% ethanol water, heating and refluxing extraction; Filter, filtering residue adds said ethanol water, heating and refluxing extraction again; Extract altogether 2 times, extracted 2 hours at every turn, extracting solution is reclaimed ethanol and is concentrated into that relative density is 1.10-1.15 85 ℃ the time; Get thick paste, be cooled to room temperature, with quite said thick paste quality 2-4 ethyl acetate extraction doubly; Extract 3 times, combining extraction liquid, with extract reclaim ethyl acetate to do not have ethyl acetate distinguish the flavor of extract.
5. the method for preparing of the glad drop pill of easypro brain of treatment cardiovascular and cerebrovascular disease according to claim 1 and 2; It is characterized in that said step (3) is: Polyethylene Glycol-6000 heating and melting in water-bath that will be equivalent to 3 times of said extract qualities; Add said extract, drip after fully mixing and process drop pill.
6. the drop pill of the method for one of claim 1-5 preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104329654A CN102488724A (en) | 2011-12-21 | 2011-12-21 | Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104329654A CN102488724A (en) | 2011-12-21 | 2011-12-21 | Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102488724A true CN102488724A (en) | 2012-06-13 |
Family
ID=46180629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104329654A Pending CN102488724A (en) | 2011-12-21 | 2011-12-21 | Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102488724A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903238A (en) * | 2005-07-28 | 2007-01-31 | 祝国光 | Extractive of traditional Chinese medicine, its prepns., preparing method and application thereof |
| CN101380343A (en) * | 2007-09-06 | 2009-03-11 | 上海医药工业研究院 | Extraction from mixture of angelica and ligusticum wallichii rhizome and extraction method |
| CN101491559A (en) * | 2008-12-17 | 2009-07-29 | 天津中新药业集团股份有限公司第六中药厂 | Method for treating drop-pills for treating heart cerebrovascular disease using supercritical extract technique |
-
2011
- 2011-12-21 CN CN2011104329654A patent/CN102488724A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1903238A (en) * | 2005-07-28 | 2007-01-31 | 祝国光 | Extractive of traditional Chinese medicine, its prepns., preparing method and application thereof |
| CN101380343A (en) * | 2007-09-06 | 2009-03-11 | 上海医药工业研究院 | Extraction from mixture of angelica and ligusticum wallichii rhizome and extraction method |
| CN101491559A (en) * | 2008-12-17 | 2009-07-29 | 天津中新药业集团股份有限公司第六中药厂 | Method for treating drop-pills for treating heart cerebrovascular disease using supercritical extract technique |
Non-Patent Citations (2)
| Title |
|---|
| 杨林等: "正交设计提取当归川芎中阿魏酸的研究", 《云南中医学院学报》 * |
| 武晓娟: ""芎归滴丸的制备及药理学研究"", 《中国人民解放军军事医学科学院 硕士学位论文》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100455291C (en) | Notoginseng medicine composition for treating cardiac and cerebral vascular diseases | |
| CN112972547A (en) | Traditional Chinese medicine composition for treating qi-blood deficiency syndrome and preparation method and application thereof | |
| CN102488725A (en) | Brain-soothing emulsion for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN101284050B (en) | Corydalis tuber water soluble part medicament and its preparation method and application | |
| CN1923241B (en) | Medicine composition containing epimedium extract, uncaria extract, and gastrodine, and its preparation and use | |
| CN106266239B (en) | Improve the composition and its preparation method and application of memory | |
| CN105031218A (en) | Traditional Chinese medicine formula for treating diabetes induced by deficiency of both qi and yin as well as preparation method and application thereof | |
| CN102100833B (en) | Drug composition for treating heart cerebrovascular diseases as well as preparation method and application thereof | |
| CN102488728A (en) | Brain-soothing capsule for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN103520684B (en) | Traditional Chinese medicine compound for reducing blood sugar | |
| CN1970050B (en) | Pharmaceutical composition for treating arrhythmia and preparation process thereof | |
| CN102488724A (en) | Brain-soothing dripping pills for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN102120000B (en) | Composition of effective parts of traditional Chinese medicine preparation for resisting Coxsackie virus B and preparation method thereof | |
| CN103393938B (en) | Traditional Chinese medicine composition for reducing blood sugar | |
| CN102204956B (en) | Chinese medicinal composition used at stroke recovery period and preparation method thereof | |
| CN105748553A (en) | Traditional Chinese medicinal composition for invigorating blood circulation, eliminating swelling and detoxifying, and preparation method thereof | |
| CN105031219A (en) | Traditional Chinese medicine compound preparation for treating diabetes induced by deficiency of both qi and yin as well as preparation method and application thereof | |
| CN102488726A (en) | Brain-soothing tablet for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN102488729A (en) | Brain-soothing aerosol for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN102488723A (en) | Brain-soothing particles for treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN101088549A (en) | Chinese medicine prepn for treating arrhythmia and its prepn process and quality control method | |
| CN102488727A (en) | Shunaoxin oral liquid used for treating cardio cerebrovascular disease and its preparation method | |
| CN102423327A (en) | Shunaoxin buccal tablet for treating cardiovascular and cerebrovascular diseases, as well as preparation method | |
| CN1034911C (en) | Shixinling oral medicine and its preparation method | |
| CN100453073C (en) | Compound radical lobelia dripping pill and its preparing method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20120613 |
|
| RJ01 | Rejection of invention patent application after publication |