CN102484982A - Oxygen concentration controllable mouse rearing box - Google Patents
Oxygen concentration controllable mouse rearing box Download PDFInfo
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- CN102484982A CN102484982A CN2010105787299A CN201010578729A CN102484982A CN 102484982 A CN102484982 A CN 102484982A CN 2010105787299 A CN2010105787299 A CN 2010105787299A CN 201010578729 A CN201010578729 A CN 201010578729A CN 102484982 A CN102484982 A CN 102484982A
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- oxygen
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- rearing
- solenoid valve
- mouse
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Abstract
The invention relates to an oxygen concentration controllable mouse rearing box which is characterized in that the box is transparent; an air pipe, a solenoid valve and an oxygen meter are arranged on the top wall of the box; the oxygen meter is connected with the solenoid valve; the air pipe for conveying oxygen enters the rearing box via the solenoid valve; and an air vent is formed on the side wall of the rearing box. When the box is used, firstly a normal range (namely lower and upper limits) is set for the oxygen meter; when the partial pressure of oxygen is lower than the lower limit, the oxygen meter gives a command, the solenoid valve is opened, and the oxygen is conveyed into the rearing box; and when the partial pressure of oxygen is higher than the upper limit, the oxygen meter gives a command, the solenoid valve is closed, and the oxygen conveying is stopped.
Description
Technical field
The present invention relates to a kind of animal facility, oxygen concentration can be controlled according to requirement of experiment automatically in the specifically a kind of mouse rearing-box, case.
Background technology
Since people such as Smith LE in 1994 with retinopathy of prematurity (Retinopathy of prematurity; ROP) mouse model (oxygen induced retinopathy; OIR) after the standardization; Many people and unit serve as the basic research of research carrier carrying out with this model all, for more deep understanding with prevent and treat this type disease, have played very big impetus.The method of making this animal model at present has following 3 kinds: the one, to being interrupted artificial oxygenation in the mouse rearing-box; Be interrupted oxygen concentration in the mensuration rearing-box with oxygen analyser, the 2nd, raise mouse with infant incubator, the 3rd, raise mouse with hyperbaric oxygen chamber; But all there are a lot of realistic problems in these methods: with the method for oxygen concentration in oxygen analyser discontinuous measurement rearing-box and the infant incubator; Be difficult to keep the stable of the interior oxygen concentration of rearing-box, waste time and energy, also cause experimental result deviation to occur easily; The infant incubator cost is high in addition, and general Study personnel are difficult to sufficient experiment fees and buy this equipment; Hyperbaric oxygen chamber control oxygen ability is the most desirable; But to buy the possibility of expensive instrument like this littler for zoopery specially, simultaneously, because the high cost that instrument runs well; Even if it is also little to use the possibility of this equipment, unite the uniformity that use is difficult to guarantee different experimental conditions again with other experiment.
Summary of the invention
The objective of the invention is to: for such as the OIR animal model, provide that a kind of cost is low, controllability good, the mouse rearing-box of practical controlled oxygen concentration.
The present invention is achieved in that
Rearing-box 1 is transparent; Snorkel 4, oxygen analyser 5 and magnetic valve 6 are arranged; Oxygen analyser 5 is connected with magnetic valve 6; The snorkel 4 of delivering oxygen gets into rearing-box 1 through magnetic valve 6.Contain alarm in the oxygen analyser 5.
Rearing-box 1 usefulness transparent material is processed, and can be that glass or lucite, plastic material are processed.
Rearing-box 1 sidewall is provided with gateway 2, ventilating opening 3, and a valve 7 is installed at the ventilating opening place, is used to control the ventilating opening size.
During use, set oxygen analyser normal range (NR) (being lower limit and higher limit), when partial pressure of oxygen was lower than lower limit, oxygen analyser sent instruction, and alarm sends chimes of doom, and magnetic valve is open simultaneously, begins in rearing-box, to import oxygen; When partial pressure of oxygen was higher than higher limit, oxygen analyser sent instruction, and alarm sends chimes of doom, and closed electromagnetic valve stops to import oxygen simultaneously, finally keeps partial pressure of oxygen in normal range (NR).
Description of drawings
Fig. 1 is a unitary construction sketch map of the present invention
Among the figure: the 1st, rearing-box, the 2nd, gateway, the 3rd, ventilating opening, the 4th, snorkel, the 5th, oxygen analyser, the 6th, magnetic valve, the 7th, valve, the 8th, oxygen cylinder.
Embodiment
Below in conjunction with embodiment of accompanying drawing narration, the present invention is further specified.
The rearing-box 1 usefulness lucite of this embodiment is processed, and the antetheca of rearing-box is provided with gateway 2, and sidewall is provided with ventilating opening 3; The snorkel 4 of delivering oxygen gets into rearing-box 1 through magnetic valve 6; KY-2F type numeral shows that oxygen analyser 5 is connected with magnetic valve 6.A valve 7 is installed at ventilating opening 3 places, control ventilating opening 3 sizes.
Of the present inventionly manufacture process and operation sequence is following:
1) make lucite rearing-box 1, the antetheca of case is provided with the gateway 2 that mouse is raised cage, and sidewall has ventilating opening 3, and a valve 7 is installed at the ventilating opening place, is used to control the ventilating opening size.
2) snorkel 4 to rearing-box 1 delivering oxygen gets into rearing-box through magnetic valve 6.
3) oxygen analyser 5 is connected with magnetic valve 6.Setting the oxygen analyser normal range (NR) is 74%～76%, and when partial pressure of oxygen was lower than 74%, oxygen analyser sent instruction; Alarm sends chimes of doom, and magnetic valve is open simultaneously, begins in rearing-box, to import oxygen; When partial pressure of oxygen was higher than 76%, oxygen analyser sent instruction, and alarm sends chimes of doom; Closed electromagnetic valve stops to import oxygen simultaneously, finally keeps partial pressure of oxygen 74%～76%.
4) authentication method
(1) delivering oxygen in rearing-box; Be lower than 74%, 74～76% and be higher than 76% 3 interval; Use oxygen analyser on standard oximetry appearance and the rearing-box respectively, measure the interior oxygen partial pressure value of oxygen case simultaneously each 3 times, 9 times altogether; The difference of two groups of data relatively, P＞0.05 o'clock judge that the two measures difference not statistically significant.
(2) together with the 7th day C57BL/6J mouse of 3 new lives and female mouse; Be put in the oxygen case and raised 5 days continuously, in home, raised 5 days, the 17th day with ketamine and Sai La piperazine anesthesia newborn mice; Separate and cut eyeball, pathology section examination has or not the new vessels of breaking through layer of retina,limiting,internal to generate.
Claims (4)
1. the mouse rearing-box of controlled oxygen concentration has gateway (2), it is characterized in that: rearing-box (1) is transparent; Snorkel (4), oxygen analyser (5) and magnetic valve (6) are arranged; Oxygen analyser (5) is connected with magnetic valve (6); The snorkel of delivering oxygen (4) gets into rearing-box (1) through magnetic valve (6).
2. according to the said mouse rearing-box of claim 1, it is characterized in that: rearing-box (1) can be that glass or lucite, plastic material are processed.
3. according to the said mouse rearing-box of claim 1, it is characterized in that: rearing-box (1) sidewall is provided with ventilating opening (3), and a valve (7) is installed at the ventilating opening place.
4. according to the said mouse rearing-box of claim 1, it is characterized in that: oxygen analyser contains alarm in (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105787299A CN102484982A (en) | 2010-12-06 | 2010-12-06 | Oxygen concentration controllable mouse rearing box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105787299A CN102484982A (en) | 2010-12-06 | 2010-12-06 | Oxygen concentration controllable mouse rearing box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102484982A true CN102484982A (en) | 2012-06-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105787299A Pending CN102484982A (en) | 2010-12-06 | 2010-12-06 | Oxygen concentration controllable mouse rearing box |
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| CN (1) | CN102484982A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075881A (en) * | 2015-07-28 | 2015-11-25 | 无锡东晟生物科技有限公司 | Transparent pet raising case |
Citations (9)
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| JP2001095411A (en) * | 1999-09-27 | 2001-04-10 | Taiyo Denshi Kk | Hospital house (cage) for small animals |
| US20030154976A1 (en) * | 2002-02-20 | 2003-08-21 | Xenogen Corporation | Multiple output anesthesia system |
| CN1887068A (en) * | 2006-07-26 | 2007-01-03 | 温州医学院 | SPF level intermittently anoxic rat feeding compartment |
| CN2907570Y (en) * | 2006-06-15 | 2007-06-06 | 中国人民解放军新疆军区联勤部药品仪器检验所 | Plateau-simulation animal experiment chamber |
| CN101125101A (en) * | 2007-08-16 | 2008-02-20 | 首都医科大学附属北京朝阳医院 | Normal pressure low oxygen toxicity contaminated system |
| CN101491477A (en) * | 2009-03-10 | 2009-07-29 | 中国医学科学院阜外心血管病医院 | Animal experiment device capable of providing intermittence low-oxygen environment |
| CN101642385A (en) * | 2008-08-04 | 2010-02-10 | 陈锐 | Intermittent hypoxia device for experimental animals |
| CN201409995Y (en) * | 2009-06-08 | 2010-02-24 | 中国人民解放军第三军医大学第二附属医院 | Full-automatic constant pressure low oxygen high carbon clean animal culture cabin |
| CN201976562U (en) * | 2010-12-06 | 2011-09-21 | 陕西省眼科研究所 | Mouse terrarium with controllable oxygen concentration |
-
2010
- 2010-12-06 CN CN2010105787299A patent/CN102484982A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001095411A (en) * | 1999-09-27 | 2001-04-10 | Taiyo Denshi Kk | Hospital house (cage) for small animals |
| US20030154976A1 (en) * | 2002-02-20 | 2003-08-21 | Xenogen Corporation | Multiple output anesthesia system |
| CN2907570Y (en) * | 2006-06-15 | 2007-06-06 | 中国人民解放军新疆军区联勤部药品仪器检验所 | Plateau-simulation animal experiment chamber |
| CN1887068A (en) * | 2006-07-26 | 2007-01-03 | 温州医学院 | SPF level intermittently anoxic rat feeding compartment |
| CN101125101A (en) * | 2007-08-16 | 2008-02-20 | 首都医科大学附属北京朝阳医院 | Normal pressure low oxygen toxicity contaminated system |
| CN101642385A (en) * | 2008-08-04 | 2010-02-10 | 陈锐 | Intermittent hypoxia device for experimental animals |
| CN101491477A (en) * | 2009-03-10 | 2009-07-29 | 中国医学科学院阜外心血管病医院 | Animal experiment device capable of providing intermittence low-oxygen environment |
| CN201409995Y (en) * | 2009-06-08 | 2010-02-24 | 中国人民解放军第三军医大学第二附属医院 | Full-automatic constant pressure low oxygen high carbon clean animal culture cabin |
| CN201976562U (en) * | 2010-12-06 | 2011-09-21 | 陕西省眼科研究所 | Mouse terrarium with controllable oxygen concentration |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075881A (en) * | 2015-07-28 | 2015-11-25 | 无锡东晟生物科技有限公司 | Transparent pet raising case |
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Application publication date: 20120606 |