CN102477409A - Culture medium for promoting suspension growth of purple non-sulfur bacteria - Google Patents
Culture medium for promoting suspension growth of purple non-sulfur bacteria Download PDFInfo
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- CN102477409A CN102477409A CN2011102592166A CN201110259216A CN102477409A CN 102477409 A CN102477409 A CN 102477409A CN 2011102592166 A CN2011102592166 A CN 2011102592166A CN 201110259216 A CN201110259216 A CN 201110259216A CN 102477409 A CN102477409 A CN 102477409A
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- culture medium
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- tween
- bacteria
- purple
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- 241000131970 Rhodospirillaceae Species 0.000 title claims abstract description 26
- 239000000725 suspension Substances 0.000 title claims abstract description 9
- 239000001963 growth medium Substances 0.000 title abstract description 11
- 230000001737 promoting effect Effects 0.000 title 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims abstract description 13
- 229920000053 polysorbate 80 Polymers 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 8
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019797 dipotassium phosphate Nutrition 0.000 claims description 4
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 239000001632 sodium acetate Substances 0.000 claims description 4
- 235000017281 sodium acetate Nutrition 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000000843 powder Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 6
- 238000012258 culturing Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 4
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 9
- 238000005286 illumination Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 6
- 241001052560 Thallis Species 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 102100039556 Galectin-4 Human genes 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 235000017471 coenzyme Q10 Nutrition 0.000 description 1
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940035936 ubiquinone Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
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Abstract
The invention discloses a culture medium which is improved to effectively promote suspension growth of purple non-sulfur bacteria. The technical scheme is that 0.2-1% of Tween 80 is added into a conventional culture medium. The culture medium is adopted to culture the purple non-sulfur bacteria, the problem of precipitation in the growth process of the bacteria can be effectively solved, and compared with the conventional culture medium, the culture medium for culturing the bacteria has higher final culture concentration and bacterial liquid OD660To 3.5.
Description
Technical field
The invention belongs to microbiology, microecology field.Specifically, relate to a kind of substratum that promotes the purple nonsulfur bacteria suspension growth.
Background technology
Purple nonsulfur bacteria belong to Rhodospirillaceae (Rhodospirillaceae) be one type change various purple photosynthetic bacterium (phototrophic purple bacteria, PNS).Can have abundant metabolism diversity with different physiological metabolism mode growth and breedings under anaerobism illumination, aerobic dark condition.Because protein contnt is up to more than 60% in its cell dry-matter, its gal4 amino acid ratio of components is more complete, and it is very abundant also to contain multivitamin, especially vitamin B group in the cell, Vb
2, folic acid, pantothenic acid, vitamin H content also higher, also contain physiologically active substances such as a large amount of carrotenoid, ubiquinone simultaneously.Therefore have very high nutritive value, this just it in aquaculture as bait and as the feed adding component basic substance.Simultaneously, thalline can utilize effectively in process of growth that organism effectively reduces water body COD value in the water body, realizes the dual function of aquatic feeds and water treatment.
The key of purple nonsulfur bacteria promotion and application is that the thalline of cultivating should have higher cell concentration, present conventional substratum: main prescription is: ammonium chloride 1.0g, sodium acetate 3.5g; Magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g; Potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI; PH7.2. use this culture medium prescription thalline in culturing process deposition growth phenomenon can occur, influence the microenvironment of thalli growth, be unfavorable for the raising of thalline total concn.
Summary of the invention
For overcoming the above problems, the invention provides a kind of promotion purple nonsulfur bacteria suspension growth substratum, purple nonsulfur bacteria substratum of the present invention can effectively promote the thalline suspension growth, improves thalline and finally cultivates concentration.
The technical scheme that the present invention adopts is: in aforementioned conventional substratum, add 0.2%～1% tween 80, promptly every 1000ml minimum medium adds 2～10ml tween 80.The preferred every 1000ml of substratum adds the 10ml tween 80.
Regulating medium pH of the present invention is 7.2,121 ℃ of sterilizations 25 minutes, and purple nonsulfur bacteria is inoculated in the substratum, places illumination box, illumination condition 2000lux, 32 ℃ of temperature.
The purple nonsulfur bacteria deposited phenomenon weakens with the increase of tween 80, when every 1000ml adds the 10ml tween 80, does not occur deposited phenomenon basically, cultivates 6 days OD
660Reach 3.5, continuing to increase tween 80 has restraining effect to thalli growth, therefore selects for use the interpolation of 10ml/L tween 80 more suitable, can suitably increase according to practical situation but consider to add cost.
Beneficial effect of the present invention:
1, adopt culture medium culturing purple nonsulfur bacteria of the present invention, the maximum growth amount obviously improves.Be embodied in the OD of the more conventional culture medium culturing thalline of purple nonsulfur bacteria that adopts culture medium culturing of the present invention
660Ultimate density provides more than 70%, adopts the final OD of substratum thalline of the present invention
660Can reach more than 3.5, see a in the accompanying drawing; And conventional substratum generally only can reach 1.9～2.0, sees b in the accompanying drawing.
2, be used for the purification of aquaculture system with the purple nonsulfur bacteria of culture medium culturing of the present invention, effect is obvious.20kg/hm by purple nonsulfur bacteria
2The disposable fish pond that is sprinkled into of consumption, the test tank comparison improves obviously according to the pond, and COD descends 40%, and ammonia nitrogen reduces 55.6%
Description of drawings
Substratum of the present invention (add 10ml tween 80/L) and conventional substratum, in the process of cultivating purple nonsulfur bacteria, optical density(OD) OD
660Variation diagram.A representative invention substratum, b represent conventional substratum in the accompanying drawing.
Embodiment
Embodiment 1:
(1) promotes the preparation of purple nonsulfur bacteria suspension growth substratum
Minimum medium: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2. add tween 80 5ml in above-mentioned substratum.
(2) cultivation of purple nonsulfur bacteria
Above-mentioned medium pH is adjusted to 7.2,121 ℃ of sterilizations 25 minutes, purple nonsulfur bacteria is inoculated in the substratum by culture volume 3%, place illumination box, illumination condition 2000lux, 32 ℃ of temperature.
Cultivation results: illumination cultivation is after 6 days, and thalli growth gets into stationary phase, no thalline deposited phenomenon in the bacterium liquid, bacterium liquid OD
660Arrive 3.0
Embodiment 2:
(1) promotes the preparation of purple nonsulfur bacteria suspension growth substratum
Minimum medium: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2. add tween 80 10ml in above-mentioned substratum.
(2) cultivation of purple nonsulfur bacteria
Above-mentioned medium pH is adjusted to 7.2,121 ℃ of sterilizations 25 minutes, purple nonsulfur bacteria is inoculated in the substratum by culture volume 3%, place illumination box, illumination condition 2000lux, 32 ℃ of temperature.
Cultivation results: illumination cultivation is after 4 days, and thalli growth gets into stationary phase, bacterium liquid OD
660Arrive 3.5.
Claims (2)
1. one kind promotes purple nonsulfur bacteria suspension growth substratum, and its characteristic is made up of conventional substratum, tween 80.Every 1000ml adds 0.2%～1% tween 80 in conventional substratum.Conventional substratum is: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2.
2. according to the requirement of right 1, the purple nonsulfur bacteria bacterium liquid or the dry powder formulations of cultivation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102592166A CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium for promoting suspension growth of purple non-sulfur bacteria |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102592166A CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium for promoting suspension growth of purple non-sulfur bacteria |
Publications (1)
| Publication Number | Publication Date |
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| CN102477409A true CN102477409A (en) | 2012-05-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011102592166A Pending CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium for promoting suspension growth of purple non-sulfur bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911313A (en) * | 2013-10-07 | 2014-07-09 | 领绿生物镇江有限公司 | Method for fermenting photosynthetic bacteria with high density |
| CN109355193A (en) * | 2018-11-23 | 2019-02-19 | 杭州园泰生物科技有限公司 | Reduce the method that Isochrysis galbana is adherent and improves increment |
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2011
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109355193A (en) * | 2018-11-23 | 2019-02-19 | 杭州园泰生物科技有限公司 | Reduce the method that Isochrysis galbana is adherent and improves increment |
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Address after: 214135 Jiangsu Province, Wuxi City District Zhenze Wuxi Road No. 18 (National) Aquarius Software Park Room 306 Applicant after: Zhou Hao Address before: 214135 Building 5, creative innovation industrial park, Xinhua Road 101, Wuxi New District, Jiangsu, B Applicant before: Zhou Hao |
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Application publication date: 20120530 |