CN102477409A - Culture medium capable of promoting suspension growth of purple non-sulfur bacteria - Google Patents
Culture medium capable of promoting suspension growth of purple non-sulfur bacteria Download PDFInfo
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- CN102477409A CN102477409A CN2011102592166A CN201110259216A CN102477409A CN 102477409 A CN102477409 A CN 102477409A CN 2011102592166 A CN2011102592166 A CN 2011102592166A CN 201110259216 A CN201110259216 A CN 201110259216A CN 102477409 A CN102477409 A CN 102477409A
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Abstract
The invention discloses an improved culture medium capable of effectively promoting suspension growth of purple non-sulfur bacteria. The technical scheme includes that 0.2%-1% of polysorbate 80 is added into a conventional culture medium. Compared with the conventional culture medium, the culture medium for culturing the purple non-sulfur bacteria is capable of effectively solving the problem of precipitation of the bacteria during growth. The bacteria cultured by the culture medium are higher in final culturing concentration, and OD660 (optical density with 660nm wavelength) of bacterium liquor reaches 3.5.
Description
Technical field
The invention belongs to microbiology, microecology field.Specifically, relate to a kind of substratum that promotes the purple nonsulfur bacteria suspension growth.
Background technology
Purple nonsulfur bacteria belong to Rhodospirillaceae (Rhodospirillaceae) be one type change various purple photosynthetic bacterium (phototrophic purple bacteria, PNS).Can have abundant metabolism diversity with different physiological metabolism mode growth and breedings under anaerobism illumination, aerobic dark condition.Because protein contnt is up to more than 60% in its cell dry-matter, its gal4 amino acid ratio of components is more complete, and it is very abundant also to contain multivitamin, especially vitamin B group in the cell, Vb
2, folic acid, pantothenic acid, vitamin H content also higher, also contain physiologically active substances such as a large amount of carrotenoid, ubiquinone simultaneously.Therefore have very high nutritive value, this just it in aquaculture as bait and as the feed adding component basic substance.Simultaneously, thalline can utilize effectively in process of growth that organism effectively reduces water body COD value in the water body, realizes the dual function of aquatic feeds and water treatment.
The key of purple nonsulfur bacteria promotion and application is that the thalline of cultivating should have higher cell concentration, present conventional substratum: main prescription is: ammonium chloride 1.0g, sodium acetate 3.5g; Magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g; Potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI; PH7.2. use this culture medium prescription thalline in culturing process deposition growth phenomenon can occur, influence the microenvironment of thalli growth, be unfavorable for the raising of thalline total concn.
Summary of the invention
For overcoming the above problems, the invention provides a kind of promotion purple nonsulfur bacteria suspension growth substratum, purple nonsulfur bacteria substratum of the present invention can effectively promote the thalline suspension growth, improves thalline and finally cultivates concentration.
The technical scheme that the present invention adopts is: in aforementioned conventional substratum, add 0.2%～1% tween 80, promptly every 1000ml minimum medium adds 2～10ml tween 80.The preferred every 1000ml of substratum adds the 10ml tween 80.
Regulating medium pH of the present invention is 7.2,121 ℃ of sterilizations 25 minutes, and purple nonsulfur bacteria is inoculated in the substratum, places illumination box, illumination condition 2000lux, 32 ℃ of temperature.
The purple nonsulfur bacteria deposited phenomenon weakens with the increase of tween 80, when every 1000ml adds the 10ml tween 80, does not occur deposited phenomenon basically, cultivates 6 days OD
660Reach 3.5, continuing to increase tween 80 has restraining effect to thalli growth, therefore selects for use the interpolation of 10ml/L tween 80 more suitable, can suitably increase according to practical situation but consider to add cost.
Beneficial effect of the present invention:
1, adopt culture medium culturing purple nonsulfur bacteria of the present invention, the maximum growth amount obviously improves.Be embodied in the OD of the more conventional culture medium culturing thalline of purple nonsulfur bacteria that adopts culture medium culturing of the present invention
660Ultimate density provides more than 70%, adopts the final OD of substratum thalline of the present invention
660Can reach more than 3.5, see a in the accompanying drawing; And conventional substratum generally only can reach 1.9～2.0, sees b in the accompanying drawing.
2, be used for the purification of aquaculture system with the purple nonsulfur bacteria of culture medium culturing of the present invention, effect is obvious.20kg/hm by purple nonsulfur bacteria
2The disposable fish pond that is sprinkled into of consumption, the test tank comparison improves obviously according to the pond, and COD descends 40%, and ammonia nitrogen reduces 55.6%
Description of drawings
Substratum of the present invention (add 10ml tween 80/L) and conventional substratum, in the process of cultivating purple nonsulfur bacteria, optical density(OD) OD
660Variation diagram.A representative invention substratum, b represent conventional substratum in the accompanying drawing.
Embodiment
Embodiment 1:
(1) promotes the preparation of purple nonsulfur bacteria suspension growth substratum
Minimum medium: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2. add tween 80 5ml in above-mentioned substratum.
(2) cultivation of purple nonsulfur bacteria
Above-mentioned medium pH is adjusted to 7.2,121 ℃ of sterilizations 25 minutes, purple nonsulfur bacteria is inoculated in the substratum by culture volume 3%, place illumination box, illumination condition 2000lux, 32 ℃ of temperature.
Cultivation results: illumination cultivation is after 6 days, and thalli growth gets into stationary phase, no thalline deposited phenomenon in the bacterium liquid, bacterium liquid OD
660Arrive 3.0
Embodiment 2:
(1) promotes the preparation of purple nonsulfur bacteria suspension growth substratum
Minimum medium: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2. add tween 80 10ml in above-mentioned substratum.
(2) cultivation of purple nonsulfur bacteria
Above-mentioned medium pH is adjusted to 7.2,121 ℃ of sterilizations 25 minutes, purple nonsulfur bacteria is inoculated in the substratum by culture volume 3%, place illumination box, illumination condition 2000lux, 32 ℃ of temperature.
Cultivation results: illumination cultivation is after 4 days, and thalli growth gets into stationary phase, bacterium liquid OD
660Arrive 3.5.
Claims (2)
1. one kind promotes purple nonsulfur bacteria suspension growth substratum, and its characteristic is made up of conventional substratum, tween 80.Every 1000ml adds 0.2%～1% tween 80 in conventional substratum.Conventional substratum is: ammonium chloride 1.0g, sodium acetate 3.5g, magnesium chloride 0.1g, calcium chloride 0.1g, potassium primary phosphate 0.6g, potassium hydrogenphosphate 0.4g, yeast extract paste 0.1g, water 1000mI, pH7.2.
2. according to the requirement of right 1, the purple nonsulfur bacteria bacterium liquid or the dry powder formulations of cultivation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102592166A CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium capable of promoting suspension growth of purple non-sulfur bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102592166A CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium capable of promoting suspension growth of purple non-sulfur bacteria |
Publications (1)
| Publication Number | Publication Date |
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| CN102477409A true CN102477409A (en) | 2012-05-30 |
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| CN2011102592166A Pending CN102477409A (en) | 2011-09-05 | 2011-09-05 | Culture medium capable of promoting suspension growth of purple non-sulfur bacteria |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911313A (en) * | 2013-10-07 | 2014-07-09 | 领绿生物镇江有限公司 | Method for fermenting photosynthetic bacteria with high density |
| CN109355193A (en) * | 2018-11-23 | 2019-02-19 | 杭州园泰生物科技有限公司 | Reduce the method that Isochrysis galbana is adherent and improves increment |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366804A (en) * | 2001-01-26 | 2002-09-04 | 东京大学 | Artificial SHIRO method for forming Japan pine mushroom |
| JP2004275084A (en) * | 2003-03-17 | 2004-10-07 | Creatis Dam:Kk | Method for producing photosynthetic bacterium by culture using food additive |
| WO2006041903A2 (en) * | 2004-10-08 | 2006-04-20 | Coho Hydroclean Sa | A method of cultivating animals to develop a desired color and to increase their rate of growth |
| CN1775944A (en) * | 2004-11-15 | 2006-05-24 | 方强 | Photosynthetic bacterium culture and formulation |
| JP2006288221A (en) * | 2005-04-06 | 2006-10-26 | Kansai Electric Power Co Inc:The | Method for preserving photosynthetic bacterium and preserved material of photosynthetic bacterium obtained by the method |
| CN1943890A (en) * | 2006-10-27 | 2007-04-11 | 大连轻工业学院 | Method for in site microbe repairing organic polluted soil |
| CN101760480A (en) * | 2008-12-25 | 2010-06-30 | 中国科学院上海生命科学研究院 | Bio-hydrogen production method by photosynthetic bacteria |
| JP4780414B2 (en) * | 2007-02-16 | 2011-09-28 | サンコーテック株式会社 | Liquid medium and method for growing and cultivating photosynthetic bacteria |
-
2011
- 2011-09-05 CN CN2011102592166A patent/CN102477409A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1366804A (en) * | 2001-01-26 | 2002-09-04 | 东京大学 | Artificial SHIRO method for forming Japan pine mushroom |
| JP2004275084A (en) * | 2003-03-17 | 2004-10-07 | Creatis Dam:Kk | Method for producing photosynthetic bacterium by culture using food additive |
| WO2006041903A2 (en) * | 2004-10-08 | 2006-04-20 | Coho Hydroclean Sa | A method of cultivating animals to develop a desired color and to increase their rate of growth |
| WO2006041903A3 (en) * | 2004-10-08 | 2007-01-04 | Coho Hydroclean Sa | A method of cultivating animals to develop a desired color and to increase their rate of growth |
| CN1775944A (en) * | 2004-11-15 | 2006-05-24 | 方强 | Photosynthetic bacterium culture and formulation |
| JP2006288221A (en) * | 2005-04-06 | 2006-10-26 | Kansai Electric Power Co Inc:The | Method for preserving photosynthetic bacterium and preserved material of photosynthetic bacterium obtained by the method |
| CN1943890A (en) * | 2006-10-27 | 2007-04-11 | 大连轻工业学院 | Method for in site microbe repairing organic polluted soil |
| JP4780414B2 (en) * | 2007-02-16 | 2011-09-28 | サンコーテック株式会社 | Liquid medium and method for growing and cultivating photosynthetic bacteria |
| CN101760480A (en) * | 2008-12-25 | 2010-06-30 | 中国科学院上海生命科学研究院 | Bio-hydrogen production method by photosynthetic bacteria |
Non-Patent Citations (3)
| Title |
|---|
| 张东向等: "DMSO和Tween280对黄芩悬浮细胞系的影响", 《种子》 * |
| 杨素萍等: "一株可利用硫化物的紫色硫细菌的分离与鉴定", 《山西大学学报》 * |
| 钱森和等: "紫色非硫光合细菌培养基条件优化的研究", 《安徽工程科技学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911313A (en) * | 2013-10-07 | 2014-07-09 | 领绿生物镇江有限公司 | Method for fermenting photosynthetic bacteria with high density |
| CN109355193A (en) * | 2018-11-23 | 2019-02-19 | 杭州园泰生物科技有限公司 | Reduce the method that Isochrysis galbana is adherent and improves increment |
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Address after: 214135 Jiangsu Province, Wuxi City District Zhenze Wuxi Road No. 18 (National) Aquarius Software Park Room 306 Applicant after: Zhou Hao Address before: 214135 Building 5, creative innovation industrial park, Xinhua Road 101, Wuxi New District, Jiangsu, B Applicant before: Zhou Hao |
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Application publication date: 20120530 |