CN102455281B - High flux multipurpose liquid sample rapid photoelectric analyzer - Google Patents
High flux multipurpose liquid sample rapid photoelectric analyzer Download PDFInfo
- Publication number
- CN102455281B CN102455281B CN 201110070338 CN201110070338A CN102455281B CN 102455281 B CN102455281 B CN 102455281B CN 201110070338 CN201110070338 CN 201110070338 CN 201110070338 A CN201110070338 A CN 201110070338A CN 102455281 B CN102455281 B CN 102455281B
- Authority
- CN
- China
- Prior art keywords
- layer
- photodetector
- light
- test paper
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Abstract
The invention relates to a high flux multipurpose liquid sample rapid photoelectric analyzer, which comprises test paper, a multiband reflectance spectrum apparatus, a photoelectric detector, a controller and a high speed centrifuger; wherein the test paper, the multiband reflectance spectrum apparatus, the photoelectric detector, the controller and the high speed centrifuger enable mutual cooperated operation for completing the rapid analysis and detection of the sample rapid; by photoelectric signal induction transmission, the photoelectric analyzer possesses rapid, highly efficient, accurate and reliable detection technology.
Description
Technical field
The present invention relates to a kind of analyzing and testing instrument, particularly relate to a kind of high flux multipurpose liquid sample Quick photoelectric analyser.
Background technology
The analyzing and testing instrument is the sample detection instrument of a kind of solid that relates to medical treatment, chemistry, physics and the fields such as biological, liquid, gas.And present method of testing and detection and proving installation can not satisfy the express-analysis detection of high flux multipurpose liquid sample far away at aspects such as identity, reliability, accuracy, sensitivitys; Wherein, traditional test paper is to apply a kind of chemical reaction reagent in paper substrates, also applies simultaneously when needed a kind of developer.When the evaluating objects composition in the sample liquids exists and reaches certain content when above, this target component and chemical reagent reaction, the result product of reaction passes through chromogenic reagent, to realize the qualitative and half-quantitative detection of this target component.Therefore the way of test paper analysis has simple, fast and easy advantage.But some shortcomings are arranged also.As can not carrying out quantitative test, when having other composition with the target component similar performance to exist in the sample, the result can be interfered even complete mistake, must separate etc. in advance.
Publication number is that the application for a patent for invention of CN1685226 discloses a kind of high-resolution granulomere divisional processing and separation detecting system, and this system can comprise the flow cytometer that resolution strengthens.In one embodiment, this invention can comprise at least one fluid source conduit, and it can import the fluid source and course in the nozzle of resolution enhancing at a certain angle, and described angle can strengthen the resolution of particle by cell sensing systems; But the deficiency such as it exists, and detection sensitivity is lower, instrument is bulky, complicated operation, expensive, sample and reagent dosage are larger.
Publication number is that the application for a patent for invention of CN1330151 discloses a kind of flow cytometer based on microflow control technique, regulates platform, signal treatment box, stube cable, main control system etc. by base, cabinet, micro-fluidic chip, optical detection unit, fluid IO interface, micrometric displacement and forms.Base is with fixing on the cabinet, micro-displacement platform is fixed on the base, micro-fluidic chip is fixed on the micro-displacement platform, the fluid IO interface is connected with micro-fluidic chip by platform, optical detection unit is positioned at the top of micro-fluidic chip, finish optical detection by regulating relative position, the separation unit on the chip is finished sorting work simultaneously; But its exist detecting reliability lower,, the deficiency such as complicated operation, field tests scope be less.
In addition, the modern instrumental analysis way by the integrated use of various rigorous analysis detection techniques, can realize the precision of the sample of these biogenetic derivations is separated and the detection of pin-point accuracy.For example, use various physics and chemistry Analytical Separation, precision chemical analysis technology, gas chromatography and efficient liquid phase chromatographic analysis, various modern precision detecting device, mass spectrum and spectral technique, usually can realize the content of various content of chemical substances, virosis bacterial content and other various physiology compositions in the various biological samples is detected accurately.
The method that above-mentioned integrated use modern analysis means Realization analysis detects has accurate, reliable advantage.But when analyzing a complex sample, need to employ several technician, use multiple technologies means that one or several large-scale experiment chambers just can have, take a long time to finish Detection task.Sometimes in specific situation, in the time of need to finishing fast the detection of a biogenetic derivation sample, the modern analysis means can be unable to do what one wishes.For example, in the situation that customs quarantine control, diagnosis, epidemic infectious diseases outburst, Environment Pollution Event or food safety affair occur, need fast detecting, in order to make rapidly corresponding decision, diagnosis and treatment.Even in medical science and biological study, although as if the detection of each sample so not urgent, when having a large amount of samples to study simultaneously, can realize fast that sample analysis has also become to finish one of necessary condition of Task with detecting.
Therefore, a kind of compartment analysis and the detection of biogenetic derivation sample can being combined can be fast, efficient, and accurately, reliable detection technique, will not only satisfy the demand of fast detecting, also will bring great variation for medical science and biological research.The material resources of in the past need to employing a tremendous amount of manpower and time also are difficult to finish scientific research, and possibility will become along with the application of Fast Detection Technique.The impact of this variation will be revolutionary.
Summary of the invention
Technology that the present invention introduces a kind of " Lab on Paper (LOP) ", i.e. " laboratory on the paper " technology, just be based on a kind of like this thinking, the step of compartment analysis and accurately detection is united two into one, be integrated in a miniature instrument, succinct to realize, reliable, accurate, at a high speed, medicine detects and other biogenetic derivation sample analysis testing process efficiently.
The invention provides a kind of high flux multipurpose liquid sample express-analysis detector, be used for the fluid sample that the on-the-spot medical treatment of express-analysis, environmental monitoring, food inspection, biology and molecular biology experiment are collected, such as qualitative, sxemiquantitative and the quantitative test of one or several compositions in blood, urine, tissue fluid, fruit and vegetable juice and the extract.Technology that this quick analytic instrument is based on a kind of " laboratory on the paper ", the employed instrument of this technology mainly is comprised of following several parts: the test paper of sandwich construction, multiband Reflection Spectrometer, photodetector, controller and supercentrifuge.
The objective of the invention is for the deficiencies in the prior art, provide a kind of high flux express-analysis detecting instrument in " laboratory on the paper " technical foundation, be used for the multicomponent analyzing and testing of fluid sample such as blood, urine, tissue fluid, fruit and vegetable juice, biology and environmental samples extract.
The high flux multipurpose liquid sample Quick photoelectric analyser that the present invention relates to comprises test paper, multiband Reflection Spectrometer, photodetector, controller and supercentrifuge; Wherein, described test paper, multiband Reflection Spectrometer, photodetector, controller and the mutual collaborative work of supercentrifuge five parts, and be done in such a manner fluid sample express-analysis testing process:
(1) described test paper has six layers of structure, is followed successively by transparent substrates layer, color layer, responding layer, filtering layer, separating layer and sample layer; Described transparent substrates layer is made by the high transparent polyaniline macromolecular compound of colored intensity, or makes with the high high-intensity inorganic glass material of colored intensity; Described color layer is oval, and color layer is made by the photochromics that contains leuco dye and developer; Described responding layer is trapezoidal, and described trapezoidal area is identical with the oval-shaped area of color layer, responding layer contains number of chemical reaction or biochemical reaction reagent, wherein, described number of chemical reaction or biochemical reaction reagent are evenly distributed on the non-touching zones of different of responding layer, and described distribution forms an array; Described filtering layer is triangle, and described leg-of-mutton area is identical with the trapezoidal area of responding layer, and the reaction reagent of scattering and permeating in the responding layer is filtered; Described separating layer is formed by nano inorganic oxide particle and the high-intensity nano inorganic oxide fiber combinations of high porosity, and the parts by weight ratio of the nano inorganic oxide particle of high porosity and high-intensity nano inorganic oxide fiber is 1: 0.01 to 1: 0.015;
(2) described multiband Reflection Spectrometer comprises P-polarization alternative light source, angle of light imaging inductor, considers light device, sample synchronous transport and analytic system, reflected light correction and control system, signal detector, data analysis and process software; Wherein, described P-polarization alternative light source comprises that laser instrument, optical beam expander, polarizer and number are the semiconductor light-emitting-diode of 3-128 different wavelength, and described wavelength is between 240nm and 1600nm; Described polarizer is crystal structure, can polarization light output, and before polarizer is built in optical beam expander in the described P-polarization alternative light source; Described angle of light imaging inductor is many interfaces transmission-reflection configuration, can receive the array in linearly polarized light and the in real time induction above-mentioned (1); Described light filter is 1mm2 to 64 areas by 4 square optical filtering thin slice forms that light filter is installed on the runner of described supercentrifuge, and described runner is controlled by sample synchronous transport and analytic system in the described multiband Reflection Spectrometer;
(3) semiconductor light-emitting-diode of the 3-128 in above-mentioned (2) different wavelength is arranged in circle, concentric with the central shaft of described photodetector, so that each semiconductor light-emitting-diode and photodetector optics are symmetrical, and the semiconductor light-emitting-diode of 3-128 different wavelength is connected with described controller respectively independently, so that the luminous of each semiconductor light-emitting-diode independently control, to guarantee the rotor real-time synchronization with photodetector;
(4) described controller is also controlled the rotor speed of photodetector in real time, reads the angle that relatively rotates of rotor, when the separating layer of described test paper is alignd with the rotor of photodetector, triggers the luminous and photodetector sampling of P-polarization alternative light source;
(5) Image Acquisition in the described photodetector and process software are to the pixel summation of the zones of different in above-mentioned (2), and described zones of different is corresponding with the image space of the corresponding reagent areas of test paper; Then, the Image Acquisition in the described photodetector and process software are to the summing value storage of the pixel of described zones of different, and the numerical value of each storage has corresponding relevant sample time.
As an optimal way, the high-intensity nano inorganic oxide fiber outside surface in above-mentioned (1) is coated with the nano-oxide particles of organic compound.
As an optimal way, the wavelength of the semiconductor light-emitting-diode in above-mentioned (2) is 640nm or 800nm.
As an optimal way, the square optical filtering thin slice that the light filter in above-mentioned (2) is 1mm2 by 32 areas forms.
The using method of express-analysis detecting instrument of the present invention is as follows:
With bleed liquid, urine, tissue fluid, fruit and vegetable juice or an extract, or the fluid sample to be detected of other any kind drips on the sample aspect of test paper, then as requested test paper is placed on the ad-hoc location in the instrument, starts instrument.Instrument is done the high speed centrifugation motion with this test paper, so the reagent that the composition in the sample sees through separating layer successively, reach chemical reaction layer and there reacts, reaction result develops the color through color layer, by having carried out the result that synchronous reflectance spectrum is read colour developing with the hydro-extractor motion.
Description of drawings
Fig. 1 is the structural representation of multilayer among the present invention " test paper ".
Fig. 2 is the structure summary schematic diagram of Quick photoelectric analyser among the present invention.
Fig. 3 is the fundamental diagram of Quick photoelectric analyser among the present invention.
Wherein: 1 is sample layer; 2 is separating layer; 3 is filtering layer; 4 is responding layer; 5 is color layer; 6 is the transparent substrates layer; 7 is test paper; 8 is supercentrifuge; 9 is photodetector 1; 10 is photodetector N; 11 is the multiband Reflection Spectrometer.
Embodiment
Below in conjunction with embodiment and accompanying drawing instrument provided by the invention is described in further detail, but does not therefore limit the present invention.
Embodiment 1:
Urine routine: the structure of special test paper and composition, the chemical reaction layer of this test paper is coated with 16 kinds of reagent according to the modes of 4 rows, 4 row, and wherein 4 kinds is the blank reagent of usefulness as a comparison.A urine is dropped on the sample layer, on the position that the instrument that this test paper is put into is stipulated, start instrument.Need approximately 10 minutes, instrument provides the report of 12 kinds of indexs in the urine, finishes urine routine.
Embodiment 2:
Haematogenic immunity checks: the structure of special test paper and composition, the chemical reaction layer of this test paper is coated with reagent according to the mode of 4 rows, 4 row 16 positions, wherein 2 positions are the blank reagent of usefulness as a comparison, 2 positions are standard specimens, on all the other 12 positions, apply respectively according to the rules any of 4 kinds of antibody reagents.Bleed drop on sample layer with one, on the position that the instrument that this test paper is put into is stipulated, start instrument.Need approximately 10 minutes, instrument provides the report of four kinds of immune indexes of blood, finishes the haematogenic immunity inspection.
Embodiment 3:
The Determination of Organic Phosphorus Pesticides in Vegetables residual content checks: the structure of special test paper and composition, the chemical reaction layer of this test paper is coated with reagent according to the mode of 4 rows, 4 row 16 positions, wherein 4 positions are the blank reagent of usefulness as a comparison, 4 positions are standard specimens, on all the other 8 positions, apply respectively according to the rules 8 kinds for the reagent of different organophosphorus pesticides.A slice vegetables are smashed, and the water that adds same weight reconciles into sample.A sample drop on sample layer, on the position that the instrument that this test paper is put into is stipulated, is started instrument.Need approximately 10 minutes, instrument provides the report of 8 kinds of organophosphorus pesticide residual quantities of blood, finishes the organophosphorus pesticide inspection.
Claims (4)
1. a high flux multipurpose liquid sample Quick photoelectric analyser is characterized in that, this Quick photoelectric analyser comprises test paper, multiband Reflection Spectrometer, photodetector, controller and supercentrifuge; Wherein, described test paper, multiband Reflection Spectrometer, photodetector, controller and the mutual collaborative work of supercentrifuge five parts, and be done in such a manner fluid sample express-analysis testing process:
(1) described test paper has six layers of structure, is followed successively by transparent substrates layer, color layer, responding layer, filtering layer, separating layer and sample layer; Described transparent substrates layer is made by the high transparent polyaniline macromolecular compound of colored intensity, or makes with the high high-intensity inorganic glass material of colored intensity; Described color layer is oval, and color layer is made by the photochromics that contains leuco dye and developer; Described responding layer is trapezoidal, and described trapezoidal area is identical with the oval-shaped area of color layer, responding layer contains number of chemical reaction or biochemical reaction reagent, wherein, described number of chemical reaction or biochemical reaction reagent are evenly distributed on the non-touching zones of different of responding layer, and described distribution forms an array; Described filtering layer is triangle, and described leg-of-mutton area is identical with the trapezoidal area of responding layer, and the reaction reagent of scattering and permeating in the responding layer is filtered; Described separating layer is formed by nano inorganic oxide particle and the high-intensity nano inorganic oxide fiber combinations of high porosity, and the parts by weight ratio of the nano inorganic oxide particle of high porosity and high-intensity nano inorganic oxide fiber is 1: 0.01 to 1: 0.015;
(2) described multiband Reflection Spectrometer comprises P-polarization alternative light source, angle of light imaging inductor, light filter, sample synchronous transport and analytic system, reflected light correction and control system, signal detector, data analysis and process software; Wherein, described P-polarization alternative light source comprises that laser instrument, optical beam expander, polarizer and number are the semiconductor light-emitting-diode of 3-128 different wavelength, and described wavelength is between 240nm and 1600nm; Described polarizer is crystal structure, can polarization light output, and before polarizer is built in optical beam expander in the described P-polarization alternative light source; Described angle of light imaging inductor is many interfaces transmission-reflection configuration, can receive the array in linearly polarized light and the in real time induction above-mentioned (1); Described light filter is 1mm by 4 to 64 areas
2Square optical filtering thin slice form, light filter is installed on the runner of described supercentrifuge, described runner is controlled by sample synchronous transport and analytic system in the described multiband Reflection Spectrometer;
(3) semiconductor light-emitting-diode of the 3-128 in above-mentioned (2) different wavelength is arranged in circle, concentric with the central shaft of described photodetector, so that each semiconductor light-emitting-diode and photodetector optics are symmetrical, and the semiconductor light-emitting-diode of 3-128 different wavelength is connected with described controller respectively independently, so that the luminous of each semiconductor light-emitting-diode independently control, to guarantee the rotor real-time synchronization with photodetector;
(4) described controller is also controlled the rotor speed of photodetector in real time, reads the angle that relatively rotates of rotor, when the separating layer of described test paper is alignd with the rotor of photodetector, triggers the luminous and photodetector sampling of P-polarization alternative light source;
(5) Image Acquisition in the described photodetector and process software are to the pixel summation of the zones of different in above-mentioned (2), and described zones of different is corresponding with the image space of the corresponding reagent areas of test paper; Then, the Image Acquisition in the described photodetector and process software are to the summing value storage of the pixel of described zones of different, and the numerical value of each storage has corresponding relevant sample time.
2. high flux multipurpose liquid sample Quick photoelectric analyser according to claim 1, it is characterized in that: the high-intensity nano inorganic oxide fiber outside surface in above-mentioned (1) is coated with the nano-oxide particles of organic compound.
3. high flux multipurpose liquid sample Quick photoelectric analyser according to claim 1 and 2, it is characterized in that: the wavelength of the semiconductor light-emitting-diode in above-mentioned (2) is 640nm or 800nm.
4. high flux multipurpose liquid sample Quick photoelectric analyser according to claim 1 and 2, it is characterized in that: the light filter in above-mentioned (2) is 1mm by 32 areas
2Square optical filtering thin slice form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110070338 CN102455281B (en) | 2011-03-23 | 2011-03-23 | High flux multipurpose liquid sample rapid photoelectric analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110070338 CN102455281B (en) | 2011-03-23 | 2011-03-23 | High flux multipurpose liquid sample rapid photoelectric analyzer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102455281A CN102455281A (en) | 2012-05-16 |
| CN102455281B true CN102455281B (en) | 2013-03-13 |
Family
ID=46038705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110070338 Expired - Fee Related CN102455281B (en) | 2011-03-23 | 2011-03-23 | High flux multipurpose liquid sample rapid photoelectric analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102455281B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016536575A (en) | 2013-10-01 | 2016-11-24 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | Method for identifying the state of a sample, apparatus for analyzing a sample, and laboratory automation system |
| CN109632774A (en) * | 2018-11-22 | 2019-04-16 | 广州万孚生物技术股份有限公司 | Dry chemical detection sensor and preparation method thereof |
| CN112505027B (en) * | 2020-11-15 | 2021-11-26 | 厦门波耐模型设计有限责任公司 | Detection method, device and system of integrated detection test paper |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3932133A (en) * | 1973-07-31 | 1976-01-13 | Olympus Optical Co., Ltd. | System for detecting the particular chemical component of a test fluid |
| CN101576502A (en) * | 2009-04-08 | 2009-11-11 | 北京望升伟业科技发展有限公司 | Hemoglobin detector |
| CN201368847Y (en) * | 2009-01-14 | 2009-12-23 | 昆明光动源科技有限公司 | Test paper reflective detector |
| CN101803914A (en) * | 2009-02-18 | 2010-08-18 | 天津路德医学仪器技术有限公司 | Integrative physical examination instrument for electrocardiogram detection and urinalysis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2261673B1 (en) * | 2008-03-31 | 2018-01-24 | Terumo Kabushiki Kaisha | Method for preparing calibrating reagent, calibrating reagent, and method for calibrating blood component measuring device using said calibrating reagent |
-
2011
- 2011-03-23 CN CN 201110070338 patent/CN102455281B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3932133A (en) * | 1973-07-31 | 1976-01-13 | Olympus Optical Co., Ltd. | System for detecting the particular chemical component of a test fluid |
| CN201368847Y (en) * | 2009-01-14 | 2009-12-23 | 昆明光动源科技有限公司 | Test paper reflective detector |
| CN101803914A (en) * | 2009-02-18 | 2010-08-18 | 天津路德医学仪器技术有限公司 | Integrative physical examination instrument for electrocardiogram detection and urinalysis |
| CN101576502A (en) * | 2009-04-08 | 2009-11-11 | 北京望升伟业科技发展有限公司 | Hemoglobin detector |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102455281A (en) | 2012-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101458213B (en) | Oil species identification method by sea oil spill concentration auxiliary parameter fluorescence spectrum | |
| US6195158B1 (en) | Apparatus and method for rapid spectrophotometric pre-test screen of specimen for a blood analyzer | |
| US3555284A (en) | Multistation, single channel analytical photometer and method of use | |
| EP2309251B1 (en) | Apparatus and method for photometric testing of samples and analysis device comprising such an apparatus | |
| CN104502617A (en) | Full-automatic and high-throughput micro-fluidic chip system and method for detecting pesticide residue | |
| EP0266881A2 (en) | Method and apparatus for multiple optical assaying | |
| CN102455281B (en) | High flux multipurpose liquid sample rapid photoelectric analyzer | |
| JPS6391537A (en) | Device and method used when conducting multiple test | |
| US20190376897A1 (en) | Parallel imaging system | |
| CN202916200U (en) | Medical fluorescent quantitation analysis meter | |
| CN110806401A (en) | Wavelength/angle modulation free conversion polarized light fluorescence imaging surface plasma resonance instrument | |
| EP1794570A1 (en) | Microlaboratory for biological fluids analysis using white light illumination | |
| CN110470600A (en) | Portable water quality many reference amounts detection device and detection method | |
| DE19631855C2 (en) | Method and device for the detection of surface antigens or structural features of cells, particles or macromolecules | |
| CN101317085A (en) | Bio chip device with a sample compartment and a light sensitive element, method for the detection of fluorescent particles within at least one sample compartment of a bio chip device | |
| US20180231539A1 (en) | Device and method for detecting substances present in biological or chemical samples | |
| CN102455325B (en) | High flux multipurpose liquid sample rapid ultrasonic analyzer | |
| WO2014063912A1 (en) | Device and method for optically analyzing a material | |
| CN206095945U (en) | SERS detection device to aquatic contaminant detection | |
| Xu et al. | Milk quality control: instant and quantitative milk fat determination with a BODIPY sensor-based fluorescence detector | |
| DK180849B1 (en) | Method and device for analysis of liquid samples | |
| CN212514221U (en) | Full-spectrum miniature optical fiber spectrometer | |
| CN1281954C (en) | Apparatus for detecting special component in liquid | |
| CN101275908B (en) | Food safe monitoring instrument based on space light modulator | |
| RU2251668C2 (en) | Spectrometer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Yang Xiaobo Document name: Notification of Passing Preliminary Examination of the Application for Invention |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130313 Termination date: 20140323 |