CN102455281A - High flux multipurpose liquid sample rapid photoelectric analyzer - Google Patents
High flux multipurpose liquid sample rapid photoelectric analyzer Download PDFInfo
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- CN102455281A CN102455281A CN2011100703380A CN201110070338A CN102455281A CN 102455281 A CN102455281 A CN 102455281A CN 2011100703380 A CN2011100703380 A CN 2011100703380A CN 201110070338 A CN201110070338 A CN 201110070338A CN 102455281 A CN102455281 A CN 102455281A
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Abstract
The invention relates to a high flux multipurpose liquid sample rapid photoelectric analyzer, which comprises test paper, a multiband reflectance spectrum apparatus, a photoelectric detector, a controller and a high speed centrifuger; wherein the test paper, the multiband reflectance spectrum apparatus, the photoelectric detector, the controller and the high speed centrifuger enable mutual cooperated operation for completing the rapid analysis and detection of the sample rapid; by photoelectric signal induction transmission, the photoelectric analyzer possesses rapid, highly efficient, accurate and reliable detection technology.
Description
Technical field
The present invention relates to a kind of analyzing and testing appearance, particularly relate to a kind of high flux multi-usage fluid sample quick optical electroanalyzer.
Background technology
The analyzing and testing appearance is the sample detection instrument of a kind of solid that relates to fields such as medical treatment, chemistry, physics and biology, liquid, gas.And present method of testing and detection and proving installation can not satisfy the express-analysis detection of high flux multi-usage fluid sample far away at aspects such as identity, reliability, accuracy, sensitivitys; Wherein, traditional test paper is on paper substrates, to apply a kind of chemical reaction reagent, when needs, also applies a kind of developer simultaneously.When the evaluating objects composition in the sample liquids exists and reaches certain content when above, this target component and chemical reagent reaction, the result product of reaction passes through chromogenic reagent, to realize the qualitative and half-quantitative detection of this target component.Therefore the way of test paper analysis has simple, fast and easy advantage.But some shortcomings are arranged also.As can not carry out quantitative test, when having other composition with the target component similar performance to exist in the sample, the result can be interfered even mistake fully, must separate or the like in advance.
Publication number is that the application for a patent for invention of CN1685226 discloses a kind of high-resolution granulomere divisional processing and separation detecting system, and this system can comprise the flow cytometer that resolution strengthens.In one embodiment, this invention can comprise at least one fluid source conduit, and it can import the fluid source and course in the nozzle of resolution enhancing at a certain angle, and said angle can strengthen the resolution of particle through cell sensing systems; But it exists, and detection sensitivity is lower, instrument is bulky, complicated operation, cost an arm and a leg, deficiency such as sample and reagent dosage are bigger.
Publication number is that the application for a patent for invention of CN1330151 discloses a kind of flow cytometer based on microflow control technique, regulates platform, signal Processing case, stube cable, main control system etc. by base, cabinet, micro-fluidic chip, optical detection unit, fluid IO interface, micrometric displacement and forms.Base is with fixing on the cabinet; Micro-displacement platform is fixed on the base; Micro-fluidic chip is fixed on the micro-displacement platform, and the fluid IO interface is connected with micro-fluidic chip through platform, and optical detection unit is positioned at the top of micro-fluidic chip; Accomplish optical detection through regulating relative position, the separation unit on the chip is accomplished sorting work simultaneously; But its exist detecting reliability lower,, deficiency such as complicated operation, field tests scope be less.
In addition, the modern instrumental analysis way through the integrated use of various rigorous analysis detection techniques, can realize the precision of the sample of these biogenetic derivations is separated and the detection of pin-point accuracy.For example; Use various physics and chemical analysis isolation technics, precision chemical analysis technology, gas chromatography and efficient liquid phase chromatographic analysis, various modern precision detecting device, mass spectrum and spectral technique, can realize usually the content of various content of chemical substances, virosis bacterial content and other various physiology compositions in the various biological samples is detected accurately.
Above-mentioned integrated use modern analysis means realize the method for analyzing and testing, have accurate, reliable advantage.But when analyzing a complex sample, need employ multiple technologies means that several technician, one of utilization or several large-scale experiment chambers just can have, take a long time to accomplish the detection task.Sometimes under specific situation, when needing to accomplish the detection of a biogenetic derivation sample fast, the modern analysis means can be unable to do what one wishes.For example, under the situation that customs quarantine control, acute disease diagnosis, epidemic infectious diseases outburst, environmental pollution incident or food security incident take place, need fast detecting, so that make corresponding decision, diagnosis and treatment rapidly.Even in medical science and biological study,, when having a large amount of samples to study simultaneously, can also become to accomplish one of necessary condition of research task with detecting by the Rapid Realization sample analysis although as if the detection of each sample so not urgent.
Therefore, a kind of can the compartment analysis and the detection of biogenetic derivation sample being lumped together can be fast, efficient, and accurately, reliable detection is technological, with the demand that not only satisfies fast detecting, also will bring great variation for medical science and biological research.Need employ a tremendous amount of manpower material resources and time the past also is difficult to accomplish scientific research, and possibility will become along with the application of Fast Detection Technique.The influence of this variation will be revolutionary.
Summary of the invention
Technology that the present invention introduces a kind of " Lab on Paper (LOP) "; I.e. " laboratory on the paper " technology; Just be based on a kind of like this thinking; Compartment analysis and the step that accurately detects are united two into one, be integrated in a miniature instrument, succinct to realize, reliable, accurate, at a high speed, medicine detects and other biogenetic derivation sample analysis testing process efficiently.
The present invention provides a kind of high flux multi-usage fluid sample express-analysis detector; Be used for the fluid sample that the on-the-spot medical treatment of express-analysis, environmental monitoring, food inspection, biology and molecular biology experiment are collected, like qualitative, the sxemiquantitative and the quantitative test of a kind of or several compositions in blood, urine, tissue fluid, fruit and vegetable juice and the extract.Technology that this quick analytic instrument is based on a kind of " laboratory on the paper ", the employed instrument of this technology mainly is made up of following several parts: the test paper of sandwich construction, multiband reflectance spectrum device, photodetector, controller and supercentrifuge.
The objective of the invention is deficiency to prior art; A kind of high flux express-analysis detecting instrument is provided on " laboratory on the paper " technical foundation, is used for the multicomponent analyzing and testing of fluid sample such as blood, urine, tissue fluid, fruit and vegetable juice, biology and environmental samples extract.
The high flux multi-usage fluid sample quick optical electroanalyzer that the present invention relates to comprises test paper, multiband reflectance spectrum device, photodetector, controller and supercentrifuge; Wherein, said test paper, multiband reflectance spectrum device, photodetector, controller and the mutual collaborative work of supercentrifuge five parts, and be done in such a manner fluid sample express-analysis testing process:
(1) said test paper has six layers of structure, is followed successively by transparent substrates layer, color layer, responding layer, filtering layer, separating layer and sample layer; Said transparent substrates layer is processed by the high transparent polyaniline macromolecular compound of colored intensity, or processes with the high high-intensity inorganic glass material of colored intensity; Said color layer is oval, and color layer is processed by the photochromics that contains leuco dye and developer; Said responding layer is trapezoidal; And said trapezoidal area is identical with the oval-shaped area of color layer; Responding layer contains number of chemical reaction or biochemical reaction reagent; Wherein, described number of chemical reaction or biochemical reaction reagent are evenly distributed on the non-touching zones of different of responding layer, and said distribution forms an array; Said filtering layer is a triangle, and said leg-of-mutton area is identical with the trapezoidal area of responding layer, and the reaction reagent of scattering and permeating in the responding layer is filtered; Said separating layer is formed by the nano inorganic oxide particle and the high-intensity nano inorganic oxide fiber combinations of high porosity, and the parts by weight ratio of the nano inorganic oxide particle of high porosity and high-intensity nano inorganic oxide fiber is 1: 0.01 to 1: 0.015;
(2) said multiband reflectance spectrum device comprises P-polarization alternative light source, angle of light imaging inductor, considers light device, sample synchronous transport and analytic system, reflected light correction and control system, signal detector, data analysis and process software; Wherein, said P-polarization alternative light source comprises that laser instrument, optical beam expander, polarizer and number are the semiconductor light-emitting-diode of 3-128 different wavelength, and said wavelength is between 240nm and 1600nm; Said polarizer is a crystal structure, can polarization light output, and before polarizer is built in the optical beam expander in the said P-polarization alternative light source; Said angle of light imaging inductor is many interfaces transmission-reflection configuration, can receive the array in linearly polarized light and the induction above-mentioned (1) in real time; Said light filter is 1mm2 to 64 areas by 4 square optical filtering thin slice is formed that light filter is installed on the runner of said supercentrifuge, and said runner is by sample synchronous transport in the said multiband reflectance spectrum device and analytic system control;
(3) semiconductor light-emitting-diode of the 3-128 in above-mentioned (2) different wavelength is arranged in circle; Concentric with the central shaft of said photodetector; Make each semiconductor light-emitting-diode and photodetector optics symmetrical; And the semiconductor light-emitting-diode of 3-128 different wavelength is connected with said controller respectively independently, makes the luminous of each semiconductor light-emitting-diode independently control, and is synchronous in real time to guarantee with the rotor of photodetector;
(4) said controller is also controlled the rotor speed of photodetector in real time, reads the angle that relatively rotates of rotor, when the separating layer of said test paper is alignd with the rotor of photodetector, triggers the luminous and photodetector sampling of P-polarization alternative light source;
(5) Image Acquisition in the said photodetector and process software are to the pixel summation of the zones of different in above-mentioned (2), and said zones of different is corresponding with the image space in test paper reagent corresponding zone; Then, Image Acquisition in the said photodetector and process software are to the summing value storage of the pixel of said zones of different, and the numerical value of each storage has corresponding relevant sample time.
As an optimal way, the high-intensity nano inorganic oxide fiber outside surface in above-mentioned (1) is coated with the nano-oxide particles of organic compound.
As an optimal way, the wavelength of the semiconductor light-emitting-diode in above-mentioned (2) is 640nm or 800nm.
As an optimal way, the square optical filtering thin slice that the light filter in above-mentioned (2) is 1mm2 by 32 areas is formed.
The method of application of express-analysis detecting instrument of the present invention is following:
With bleed liquid, urine, tissue fluid, fruit and vegetable juice or an extract, or the fluid sample to be detected of other any kind of drips on the sample aspect of test paper, as requested test paper is placed on the ad-hoc location in the instrument then, starts instrument.Instrument is done the high speed centrifugation motion with this test paper; So the reagent that the composition in the sample sees through separating layer successively, reach chemical reaction layer and there reacts; Reaction result develops the color through color layer, by having carried out the result that synchronous reflectance spectrum is read colour developing with the hydro-extractor motion.
Description of drawings
Fig. 1 is the structural representation of multilayer " test paper " among the present invention.
Fig. 2 is the structure summary schematic diagram of quick optical electroanalyzer among the present invention.
Fig. 3 is the fundamental diagram of quick optical electroanalyzer among the present invention.
Wherein: 1 is sample layer; 2 is separating layer; 3 is filtering layer; 4 is responding layer; 5 is color layer; 6 is the transparent substrates layer; 7 is test paper; 8 is supercentrifuge; 9 is photodetector 1; 10 is photodetector N; 11 is multiband reflectance spectrum device.
Embodiment
Below in conjunction with embodiment and accompanying drawing instrument provided by the invention is done further to specify, but therefore do not limit the present invention.
Embodiment 1:
Urine routine: the structure of special test paper and composition, the chemical reaction layer of this test paper is coated with 16 kinds of reagent according to the mode of 4 rows, 4 row, and wherein 4 kinds is the blank reagent of usefulness as a comparison.A urine is dropped on the sample layer, on the position that the instrument that this test paper is put into is stipulated, start instrument.Need 10 minutes approximately, instrument provides the report of 12 kinds of indexs in the urine, accomplishes urine routine.
Embodiment 2:
Haematogenic immunity inspection: the structure of special test paper and composition; The chemical reaction layer of this test paper is coated with reagent according to the mode of 4 rows, 4 row on 16 positions; Wherein 2 positions are the blank reagent of usefulness as a comparison; 2 positions are standard specimens, on all the other 12 positions, apply any of 4 kinds of antibody reagents according to the rules respectively.Bleed drop on sample layer with one, on the position that the instrument that this test paper is put into is stipulated, start instrument.Need 10 minutes approximately, instrument provides the report of four kinds of immune indexes of blood, accomplishes the haematogenic immunity inspection.
Embodiment 3:
Organophosphorus pesticide residual content inspection in the vegetables: the structure of special test paper and composition; The chemical reaction layer of this test paper is coated with reagent according to the mode of 4 rows, 4 row on 16 positions; Wherein 4 positions are the blank reagent of usefulness as a comparison; 4 positions are standard specimens, on all the other 8 positions, apply 8 kinds of reagent to different organophosphorus pesticides according to the rules respectively.A slice vegetables are smashed, and the water that adds same weight reconciles into sample.A sample drop on sample layer, on the position that the instrument that this test paper is put into is stipulated, is started instrument.Need 10 minutes approximately, instrument provides the report of 8 kinds of organophosphorus pesticide residual quantities of blood, accomplishes the organophosphorus pesticide inspection.
Claims (4)
1. a high flux multi-usage fluid sample quick optical electroanalyzer is characterized in that this quick optical electroanalyzer comprises test paper, multiband reflectance spectrum device, photodetector, controller and supercentrifuge; Wherein, said test paper, multiband reflectance spectrum device, photodetector, controller and the mutual collaborative work of supercentrifuge five parts, and be done in such a manner fluid sample express-analysis testing process:
(1) said test paper has six layers of structure, is followed successively by transparent substrates layer, color layer, responding layer, filtering layer, separating layer and sample layer; Said transparent substrates layer is processed by the high transparent polyaniline macromolecular compound of colored intensity, or processes with the high high-intensity inorganic glass material of colored intensity; Said color layer is oval, and color layer is processed by the photochromics that contains leuco dye and developer; Said responding layer is trapezoidal; And said trapezoidal area is identical with the oval-shaped area of color layer; Responding layer contains number of chemical reaction or biochemical reaction reagent; Wherein, described number of chemical reaction or biochemical reaction reagent are evenly distributed on the non-touching zones of different of responding layer, and said distribution forms an array; Said filtering layer is a triangle, and said leg-of-mutton area is identical with the trapezoidal area of responding layer, and the reaction reagent of scattering and permeating in the responding layer is filtered; Said separating layer is formed by the nano inorganic oxide particle and the high-intensity nano inorganic oxide fiber combinations of high porosity, and the parts by weight ratio of the nano inorganic oxide particle of high porosity and high-intensity nano inorganic oxide fiber is 1: 0.01 to 1: 0.015;
(2) said multiband reflectance spectrum device comprises P-polarization alternative light source, angle of light imaging inductor, considers light device, sample synchronous transport and analytic system, reflected light correction and control system, signal detector, data analysis and process software; Wherein, said P-polarization alternative light source comprises that laser instrument, optical beam expander, polarizer and number are the semiconductor light-emitting-diode of 3-128 different wavelength, and said wavelength is between 240nm and 1600nm; Said polarizer is a crystal structure, can polarization light output, and before polarizer is built in the optical beam expander in the said P-polarization alternative light source; Said angle of light imaging inductor is many interfaces transmission-reflection configuration, can receive the array in linearly polarized light and the induction above-mentioned (1) in real time; Said light filter is 1mm by 4 to 64 areas
2Square optical filtering thin slice form, light filter is installed on the runner of said supercentrifuge, said runner is controlled by sample synchronous transport in the said multiband reflectance spectrum device and analytic system;
(3) semiconductor light-emitting-diode of the 3-128 in above-mentioned (2) different wavelength is arranged in circle; Concentric with the central shaft of said photodetector; Make each semiconductor light-emitting-diode and photodetector optics symmetrical; And the semiconductor light-emitting-diode of 3-128 different wavelength is connected with said controller respectively independently, makes the luminous of each semiconductor light-emitting-diode independently control, and is synchronous in real time to guarantee with the rotor of photodetector;
(4) said controller is also controlled the rotor speed of photodetector in real time, reads the angle that relatively rotates of rotor, when the separating layer of said test paper is alignd with the rotor of photodetector, triggers the luminous and photodetector sampling of P-polarization alternative light source;
(5) Image Acquisition in the said photodetector and process software are to the pixel summation of the zones of different in above-mentioned (2), and said zones of different is corresponding with the image space in test paper reagent corresponding zone; Then, Image Acquisition in the said photodetector and process software are to the summing value storage of the pixel of said zones of different, and the numerical value of each storage has corresponding relevant sample time.
2. high flux multi-usage fluid sample quick optical electroanalyzer according to claim 1, it is characterized in that: the high-intensity nano inorganic oxide fiber outside surface in above-mentioned (1) is coated with the nano-oxide particles of organic compound.
3. high flux multi-usage fluid sample quick optical electroanalyzer according to claim 1 and 2, it is characterized in that: the wavelength of the semiconductor light-emitting-diode in above-mentioned (2) is 640nm or 800nm.
4. according to the arbitrary described high flux multi-usage fluid sample quick optical electroanalyzer of claim 1-3, it is characterized in that: the light filter in above-mentioned (2) is 1mm by 32 areas
2Square optical filtering thin slice form.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201110070338 CN102455281B (en) | 2011-03-23 | 2011-03-23 | High flux multipurpose liquid sample rapid photoelectric analyzer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110070338 CN102455281B (en) | 2011-03-23 | 2011-03-23 | High flux multipurpose liquid sample rapid photoelectric analyzer |
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| CN102455281A true CN102455281A (en) | 2012-05-16 |
| CN102455281B CN102455281B (en) | 2013-03-13 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105593684A (en) * | 2013-10-01 | 2016-05-18 | 豪夫迈·罗氏有限公司 | Method for recognising state of sample, device for analysing samples and laboratory automation system |
| CN109632774A (en) * | 2018-11-22 | 2019-04-16 | 广州万孚生物技术股份有限公司 | Dry chemical detection sensor and preparation method thereof |
| CN112505027A (en) * | 2020-11-15 | 2021-03-16 | 厦门波耐模型设计有限责任公司 | Detection method, device and system of integrated detection test paper |
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| US3932133A (en) * | 1973-07-31 | 1976-01-13 | Olympus Optical Co., Ltd. | System for detecting the particular chemical component of a test fluid |
| WO2009123069A1 (en) * | 2008-03-31 | 2009-10-08 | テルモ株式会社 | Method for preparing calibrating reagent, calibrating reagent, and method for calibrating blood component measuring device using said calibrating reagent |
| CN101576502A (en) * | 2009-04-08 | 2009-11-11 | 北京望升伟业科技发展有限公司 | Hemoglobin detector |
| CN201368847Y (en) * | 2009-01-14 | 2009-12-23 | 昆明光动源科技有限公司 | Test paper reflective detector |
| CN101803914A (en) * | 2009-02-18 | 2010-08-18 | 天津路德医学仪器技术有限公司 | Integrative physical examination instrument for electrocardiogram detection and urinalysis |
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2011
- 2011-03-23 CN CN 201110070338 patent/CN102455281B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3932133A (en) * | 1973-07-31 | 1976-01-13 | Olympus Optical Co., Ltd. | System for detecting the particular chemical component of a test fluid |
| WO2009123069A1 (en) * | 2008-03-31 | 2009-10-08 | テルモ株式会社 | Method for preparing calibrating reagent, calibrating reagent, and method for calibrating blood component measuring device using said calibrating reagent |
| CN201368847Y (en) * | 2009-01-14 | 2009-12-23 | 昆明光动源科技有限公司 | Test paper reflective detector |
| CN101803914A (en) * | 2009-02-18 | 2010-08-18 | 天津路德医学仪器技术有限公司 | Integrative physical examination instrument for electrocardiogram detection and urinalysis |
| CN101576502A (en) * | 2009-04-08 | 2009-11-11 | 北京望升伟业科技发展有限公司 | Hemoglobin detector |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105593684A (en) * | 2013-10-01 | 2016-05-18 | 豪夫迈·罗氏有限公司 | Method for recognising state of sample, device for analysing samples and laboratory automation system |
| US9851301B2 (en) | 2013-10-01 | 2017-12-26 | Roche Diagnostics Operations, Inc. | Method and device for recognizing a mixed or unmixed state of a sample |
| CN109632774A (en) * | 2018-11-22 | 2019-04-16 | 广州万孚生物技术股份有限公司 | Dry chemical detection sensor and preparation method thereof |
| CN112505027A (en) * | 2020-11-15 | 2021-03-16 | 厦门波耐模型设计有限责任公司 | Detection method, device and system of integrated detection test paper |
| CN112505027B (en) * | 2020-11-15 | 2021-11-26 | 厦门波耐模型设计有限责任公司 | Detection method, device and system of integrated detection test paper |
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