CN102443616A - Method for preparing konjac glucomannan and oligo-glucomannan with different molecular weights - Google Patents
Method for preparing konjac glucomannan and oligo-glucomannan with different molecular weights Download PDFInfo
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Abstract
The invention relates to a method for preparing konjac glucomannan and oligo-glucomannan with different molecular weights. The method comprises the following steps of: rapidly adding0.1-30 parts of purified konjac glucomannan into a solution prepared by 0.001-0.01 part of glycoside hydrolase and 10-100 parts of distilled waterunder the stirring condition; reacting for 0.5-72 hours under the condition of the temperature of 30-60 DEG C and the pH of 2.0-10.0; and deactivating enzyme for 15 minutes in a boiling water bath to obtain konjac glucomannan and oligo-glucomannan aqueous solutions with different molecular weights; adding 1-30 parts of peptizing agent for stirring for 0.5-5 hours; depositing konjac glucomannan and oligo-glucomannan molecules with different molecular weights with 10-100 parts of anhydrous ethanol; after centrifugally separating sediments, adding 10-100 parts of anhydrous ethanol for strongly stirring for 0.5-5 hours; repeatedly depositing, centrifuging and stirring for 3-5 times, drying the sediment obtained by last centrifugation in a double-conic vacuum rotary drier; and grinding and screening the sediment to obtain the konjac glucomannan and the oligo-glucomannan. The method disclosed by the invention has the advantages of simplicity, low cost and wide product application.
Description
Technical field
The present invention relates to the preparation method of a kind of different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan, belong to Rhizoma amorphophalli glucomannan physics and chemical modification technique field.
Background technology
Konjaku is an Araeceae Amorphophallus per nnial herb, and its main active ingredient Rhizoma amorphophalli glucomannan (KGM) is a kind of water miscible natural macromolecule amylose, can be widely used in food, medicine, chemical industry, environmental protection and biotechnology field.The KGM relative molecular weight is 200; 000～2,000,000; So huge molecular weight makes KGM have multifrequency natures such as good gelation, thickening property, film-forming properties; But huge molecular weight makes the viscosity of KGM in water very big simultaneously, has influenced the solubleness of KGM in water, has limited the range of application of KGM.Rhizoma amorphophalli glucomannan is sufficient as a kind of renewable natural resource source, utilizes enzymatic degradation to obtain the Rhizoma amorphophalli glucomannan and the oligo-glucomannan of different molecular weight, can enlarge the range of application of renewable resources Rhizoma amorphophalli glucomannan.
Summary of the invention
Goal of the inventionThe preparation method who the purpose of this invention is to provide a kind of different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan is the further development and use service of renewable resources Rhizoma amorphophalli glucomannan.
Technical schemeTechnical scheme of the present invention is: the preparation method of a kind of different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan; It is characterized in that; It comprises following process successively: by weight taking by weighing 0.1～30 part of purifying Rhizoma amorphophalli glucomannan, 10 ~ 100 parts of zero(ppm) water, 0.0001～0.001 part of glycoside hydrolase; Separate 1～30 part of jelling agent, 30～300 parts of absolute ethyl alcohols; It is the purifying Rhizoma amorphophalli glucomannan with 0.1～30 part; Under condition of stirring, add rapidly in the solution by 0.0001～0.001 part of glycoside hydrolase and 10 ~ 100 parts of zero(ppm) water preparations; Under 30～60 ℃, pH 2.0～10.0 conditions, react 0.5～72h; The boiling water bath enzyme 15min that goes out obtains the Rhizoma amorphophalli glucomannan and the oligo-glucomannan aqueous solution of various different molecular weights; Add and separate 1～30 part of stirring of jelling agent, 0.5～5h; Rhizoma amorphophalli glucomannan and oligosaccharides molecule with 10～100 parts of absolute ethyl alcohol deposition different molecular weights; Spinning goes out post precipitation and adds the powerful 0.5～5h of stirring of 10～100 parts of absolute ethyl alcohols once more; Through 3～5 repeated precipitation-centrifugal-stirrings, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and after grinding screening, can obtain the Rhizoma amorphophalli glucomannan and the oligo-glucomannan of different molecular weight.
The preparation method of described different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan; It is characterized in that; Described different molecular weight Rhizoma amorphophalli glucomannan is meant that molecular-weight average is lower than the enzymolysis product of natural Rhizoma amorphophalli glucomannan, and described oligo-glucomannan is meant that average molecular weight range is 360～3600 enzymolysis product.
The preparation method of described different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan, its characteristic be, described purifying konjac glucomanna is that common coarse powder of glucomannan is swollen in the ethanol of concentration 50% lixiviate 2 times; With distilled water wash 3 times; Centrifuge dehydration is settled out the purifying head product with absolute ethyl alcohol again, and head product is earlier with 95% washing with alcohol; Use absolute ethanol washing at last, the product after the washing obtains through dry; Its konjac glucomanna content reaches more than 90%.
The preparation method of described different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan is characterized in that, described glycoside hydrolase is one or more the arbitrary combination in cellulase, 'beta '-mannase, the beta-glucanase.
The preparation method of described different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan is characterized in that, the described jelling agent of separating is one or more the arbitrary combination in urea, the SS.
Beneficial effectRhizoma amorphophalli glucomannan is sufficient as a kind of renewable natural resource source; Utilize enzymatic degradation to obtain the Rhizoma amorphophalli glucomannan and the oligo-glucomannan of different molecular weight; Overcome not only that natural KGM viscosity is excessive, solubleness is too small, be prone to cause the shortcoming of a series of malaise symptoms such as human body abdominal distension, diarrhoea, existing a large amount of simultaneously research confirms that oligo-glucomannans also have specific functions such as short livings bifidus bacillus, absorption pathogenic bacteria, lipopenicillinase, removing radical, the generation that suppresses the interior ammonia of intestines and absorption.Simultaneously, the Rhizoma amorphophalli glucomannan of different molecular weight and the physico-chemical property of oligo-glucomannan be different and different with molecular weight also, for industries such as food, medicine, chemical industry, material, environmental protection and biotechnology provide even more ideal raw material or additive.
Product preparation process of the present invention is simple; Cost is lower; Rhizoma amorphophalli glucomannan and the oligo-glucomannan that can obtain various different molecular weights to satisfy the processing needs of different field and direction comprehensively; And product has advantages such as color and luster is pure white, powder is even, dissolution rate is fast, gel is uniform and stable, and these all are that existing processing mode institute can not reach.
Embodiment
The present invention is realized that by following practical implementation measure wherein said raw material umber is parts by weight except that specified otherwise.Below in conjunction with embodiment the present invention is done into-goes on foot detailed description.
Rhizoma amorphophalli glucomannan purifying: common Rhizoma amorphophalli glucomannan meal is swollen in the ethanol of concentration 50% lixiviate 2 times; With distilled water wash 3 times; Centrifuge dehydration is settled out the purifying head product with absolute ethyl alcohol again, and head product is earlier with 95% washing with alcohol; Use absolute ethanol washing at last, the product after the washing can obtain the purifying Rhizoma amorphophalli glucomannan through drying.Konjac glucomanna content is more than 90%.
Embodiment 1: by weight taking by weighing 0.1 part of purifying Rhizoma amorphophalli glucomannan, 10 parts of zero(ppm) water, 0.0001 part of 'beta '-mannase.Purifying Rhizoma amorphophalli glucomannan with 0.1 part; Under condition of stirring, add rapidly in the solution by 0.0001 part of 'beta '-mannase and 10 parts of zero(ppm) water preparations; Under 30 ℃, pH 2.0 conditions, react 0.5h; The boiling water bath enzyme 15min that goes out obtains molecular-weight average and is about 600,000 konjak portuguese gansu polyose sugar aqueous solution.Add urea and stir 0.5h for 1 part; With 10 parts of absolute ethyl alcohol deposition konjak portuguese gansu polyose glycan molecules; Spinning goes out post precipitation and adds the powerful 0.5h of stirring of 10 parts of absolute ethyl alcohols once more; Through 3 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 600,000, the Rhizoma amorphophalli glucomannan of dispersion coefficient about 1.5 after sieving.
Embodiment 2: by weight taking by weighing 30 parts of purifying Rhizoma amorphophalli glucomannans, 100 parts of zero(ppm) water, 0.001 part of beta-glucanase.Purifying Rhizoma amorphophalli glucomannan with 30 parts; Under condition of stirring, add rapidly in the solution by 0.001 part of beta-glucanase and 100 parts of zero(ppm) water preparations; Under 60 ℃, pH 10.0 conditions, react 72h, the boiling water bath enzyme 15min that goes out obtains the oligo-glucomannan aqueous solution; Add SS and stir 5h for 30 parts; With 100 parts of absolute ethyl alcohol deposition oligo-glucomannan molecules; Spinning goes out post precipitation and adds the powerful 5h of stirring of 100 parts of absolute ethyl alcohols once more; Through 5 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 3600, dispersion coefficient 1.5 left and right sides oligo-glucomannans after sieving.
Embodiment 3: by weight taking by weighing 1 part of purifying Rhizoma amorphophalli glucomannan, 30 parts of zero(ppm) water, 0.0005 part of cellulase.Purifying Rhizoma amorphophalli glucomannan with 1 part; Under condition of stirring, add rapidly in the solution by 0.0005 part of cellulase and 10 parts of zero(ppm) water preparations; Under 50 ℃, pH 5.0 conditions, react 12h, the boiling water bath enzyme 15min that goes out obtains molecular-weight average and is about 300,000 konjak portuguese gansu polyose sugar aqueous solution.Add urea and stir 2h for 10 parts; With 50 parts of absolute ethyl alcohol deposition konjak portuguese gansu polyose glycan molecules; Spinning goes out post precipitation and adds the powerful 2h of stirring of 50 parts of absolute ethyl alcohols once more; Through 3 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 300,000, the Rhizoma amorphophalli glucomannan of dispersion coefficient about 1.5 after sieving.
Embodiment 4: by weight taking by weighing 10 parts of purifying Rhizoma amorphophalli glucomannans, 80 parts of zero(ppm) water, 0.001 part of beta-glucanase, 0.0001 part of 'beta '-mannase.Purifying Rhizoma amorphophalli glucomannan with 10 parts; Under condition of stirring, add rapidly in the solution by 0.001 part of beta-glucanase, 0.0001 part of 'beta '-mannase and 80 parts of zero(ppm) water preparations; Under 40 ℃, pH 8.0 conditions, react 48h; The boiling water bath enzyme 15min that goes out obtains the oligo-glucomannan aqueous solution; Add SS and stir 4h for 20 parts; With 80 parts of absolute ethyl alcohol deposition oligo-glucomannan molecules; Spinning goes out post precipitation and adds the powerful 4h of stirring of 80 parts of absolute ethyl alcohols once more; Through 4 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 360, dispersion coefficient 1.5 left and right sides oligo-glucomannans after sieving.
Embodiment 5: by weight taking by weighing 20 parts of purifying Rhizoma amorphophalli glucomannans, 90 parts of zero(ppm) water, 0.0005 part of cellulase, 0.0001 part of 'beta '-mannase.Purifying Rhizoma amorphophalli glucomannan with 20 parts; Under condition of stirring, add rapidly in the solution by 0.0005 part of cellulase, 0.0001 part of 'beta '-mannase and 90 parts of zero(ppm) water preparations; Under 40 ℃, pH 4.0 conditions, react 18h; The boiling water bath enzyme 15min that goes out obtains molecular-weight average and is about 100,000 konjak portuguese gansu polyose sugar aqueous solution.Add 10 parts in urea, 10 parts of stirrings of SS 3h; With 70 parts of absolute ethyl alcohol deposition konjak portuguese gansu polyose glycan molecules; Spinning goes out post precipitation and adds the powerful 3h of stirring of 70 parts of absolute ethyl alcohols once more; Through 3 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 100,000, the Rhizoma amorphophalli glucomannan of dispersion coefficient about 1.5 after sieving.
Embodiment 6: by weight taking by weighing 15 parts of purifying Rhizoma amorphophalli glucomannans, 70 parts of zero(ppm) water, 0.0001 part of beta-glucanase, 0.0002 part of 'beta '-mannase.Purifying Rhizoma amorphophalli glucomannan with 15 parts; Under condition of stirring, add rapidly by 0.0001 part of beta-glucanase; In the solution of 0.0002 part and 70 parts zero(ppm) water preparations of 'beta '-mannase; Under 45 ℃, pH 7.0 conditions, react 24h, the boiling water bath enzyme 15min that goes out obtains molecular-weight average and is about 50,000 konjak portuguese gansu polyose sugar aqueous solution.Add 15 parts in urea, 15 parts of stirrings of SS 4h; With 80 parts of absolute ethyl alcohol deposition konjak portuguese gansu polyose glycan molecules; Spinning goes out post precipitation and adds the powerful 4h of stirring of 80 parts of absolute ethyl alcohols once more; Through 3 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 50,000, the Rhizoma amorphophalli glucomannan of dispersion coefficient about 1.5 after sieving.
Embodiment 7: by weight taking by weighing 20 parts of purifying Rhizoma amorphophalli glucomannans, 80 parts of zero(ppm) water, 0.0003 part of beta-glucanase.Purifying Rhizoma amorphophalli glucomannan with 20 parts; Under condition of stirring, add rapidly in the solution by 0.0003 part of beta-glucanase and 80 parts of zero(ppm) water preparations; Under 50 ℃, pH 9.0 conditions, react 36h; The boiling water bath enzyme 15min that goes out obtains molecular-weight average and is about 10,000 konjak portuguese gansu polyose sugar aqueous solution; Add SS and stir 4h for 20 parts; With 70 parts of absolute ethyl alcohol deposition konjak portuguese gansu polyose glycan molecules; Spinning goes out post precipitation and adds the powerful 4h of stirring of 70 parts of absolute ethyl alcohols once more; Through 4 repeated precipitation-centrifugal-stir, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and can obtain through grinding that molecular-weight average is about 10,000, the Rhizoma amorphophalli glucomannan of dispersion coefficient about 1.5 after sieving.
Claims (5)
1. the preparation method of different molecular weight Rhizoma amorphophalli glucomannan and oligo-glucomannan; Comprise following steps successively: by weight taking by weighing 0.1～30 part of purifying Rhizoma amorphophalli glucomannan; 10 ~ 100 parts of zero(ppm) water; 0.0001～0.001 part of glycoside hydrolase is separated 1～30 part of jelling agent, 30～300 parts of absolute ethyl alcohols; It is the purifying Rhizoma amorphophalli glucomannan with 0.1～30 part; Under condition of stirring, add rapidly in the solution by 0.0001～0.001 part of glycoside hydrolase and 10 ~ 100 parts of zero(ppm) water preparations; Under 30～60 ℃, pH 2.0～10.0 conditions, react 0.5～72h; The boiling water bath enzyme 15min that goes out obtains the Rhizoma amorphophalli glucomannan and the oligo-glucomannan aqueous solution of various different molecular weights; Add and separate 1～30 part of stirring of jelling agent, 0.5～5h; Rhizoma amorphophalli glucomannan and oligosaccharides molecule with 10～100 parts of absolute ethyl alcohol deposition different molecular weights; Spinning goes out post precipitation and adds the powerful 0.5～5h of stirring of 10～100 parts of absolute ethyl alcohols once more; Through 3～5 repeated precipitation-centrifugal-stirrings, it is dry in bipyramid vacuum rotary dryer to get the last centrifugal throw out that obtains, and after grinding screening, can obtain the Rhizoma amorphophalli glucomannan and the oligo-glucomannan of different molecular weight.
2. the preparation method of different molecular weight Rhizoma amorphophalli glucomannan according to claim 1 and oligo-glucomannan; It is characterized in that; Described different molecular weight Rhizoma amorphophalli glucomannan is meant that molecular-weight average is lower than the enzymolysis product of natural Rhizoma amorphophalli glucomannan, and described oligo-glucomannan is meant that average molecular weight range is 360～3600 enzymolysis product.
3. the preparation method of different molecular weight Rhizoma amorphophalli glucomannan according to claim 1 and oligo-glucomannan, its characteristic be, described purifying konjac glucomanna is that common coarse powder of glucomannan is swollen in the ethanol of concentration 50% lixiviate 2 times; With distilled water wash 3 times; Centrifuge dehydration is settled out the purifying head product with absolute ethyl alcohol again, and head product is earlier with 95% washing with alcohol; Use absolute ethanol washing at last, the product after the washing obtains through dry; Its konjac glucomanna content reaches more than 90%.
4. the preparation method of different molecular weight Rhizoma amorphophalli glucomannan according to claim 1 and oligo-glucomannan; It is characterized in that described glycoside hydrolase is one or more the arbitrary combination in cellulase, 'beta '-mannase, the beta-glucanase.
5. the preparation method of different molecular weight Rhizoma amorphophalli glucomannan according to claim 1 and oligo-glucomannan is characterized in that, the described jelling agent of separating is one or more the arbitrary combination in urea, the SS.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103304677A (en) * | 2013-01-31 | 2013-09-18 | 重庆大学 | Method for separating and purifying konjac glucomannan |
| CN103642875A (en) * | 2013-12-10 | 2014-03-19 | 江南大学 | Method for preparing mannooligosaccharide from konjac powder |
| CN103652542A (en) * | 2012-09-10 | 2014-03-26 | 陈运忠 | Konjac dietary fiber food with high bioavailability and preparation method of konjac dietary fiber food |
| CN107260702A (en) * | 2017-07-13 | 2017-10-20 | 西南科技大学 | The preparation method of konjaku glucomannan gelatin-based capsules |
| CN107760740A (en) * | 2017-12-14 | 2018-03-06 | 宁波拜尔玛生物科技有限公司 | A kind of big sugar of high-purity kanjak mannan and preparation method thereof, application |
| CN108342430A (en) * | 2018-02-11 | 2018-07-31 | 吉林大学 | A kind of preparation method of kanjak mannan-oligosaccharides |
| CN109275918A (en) * | 2017-07-19 | 2019-01-29 | 镇安县雪樱花魔芋制品有限公司 | A kind of high-purity kanjak capsule processing technology and device |
| CN110724717A (en) * | 2019-11-07 | 2020-01-24 | 皖西学院 | Preparation method and application of bletilla striata glucomannan ester |
| CN111393536A (en) * | 2020-03-31 | 2020-07-10 | 华中农业大学 | Degradation method of konjac glucomannan |
| CN112280814A (en) * | 2020-10-29 | 2021-01-29 | 武汉芘芘薇莎生物科技有限公司 | High-content medium-low molecular weight konjac glucomannan, preparation method thereof and functional composition thereof |
| CN113215047A (en) * | 2021-05-20 | 2021-08-06 | 西北大学 | Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent |
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Cited By (16)
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| CN103652542A (en) * | 2012-09-10 | 2014-03-26 | 陈运忠 | Konjac dietary fiber food with high bioavailability and preparation method of konjac dietary fiber food |
| CN103652542B (en) * | 2012-09-10 | 2015-07-22 | 陈运忠 | Konjac dietary fiber food with high bioavailability and preparation method of konjac dietary fiber food |
| CN103304677A (en) * | 2013-01-31 | 2013-09-18 | 重庆大学 | Method for separating and purifying konjac glucomannan |
| CN103304677B (en) * | 2013-01-31 | 2015-11-25 | 重庆大学 | A kind of method of separation and purification Rhizoma amorphophalli glucomannan |
| CN103642875A (en) * | 2013-12-10 | 2014-03-19 | 江南大学 | Method for preparing mannooligosaccharide from konjac powder |
| CN107260702A (en) * | 2017-07-13 | 2017-10-20 | 西南科技大学 | The preparation method of konjaku glucomannan gelatin-based capsules |
| CN107260702B (en) * | 2017-07-13 | 2020-06-16 | 西南科技大学 | Preparation method of konjac glucomannan-gelatin-based capsule |
| CN109275918A (en) * | 2017-07-19 | 2019-01-29 | 镇安县雪樱花魔芋制品有限公司 | A kind of high-purity kanjak capsule processing technology and device |
| CN107760740A (en) * | 2017-12-14 | 2018-03-06 | 宁波拜尔玛生物科技有限公司 | A kind of big sugar of high-purity kanjak mannan and preparation method thereof, application |
| CN108342430A (en) * | 2018-02-11 | 2018-07-31 | 吉林大学 | A kind of preparation method of kanjak mannan-oligosaccharides |
| CN110724717A (en) * | 2019-11-07 | 2020-01-24 | 皖西学院 | Preparation method and application of bletilla striata glucomannan ester |
| CN111393536A (en) * | 2020-03-31 | 2020-07-10 | 华中农业大学 | Degradation method of konjac glucomannan |
| CN111393536B (en) * | 2020-03-31 | 2021-01-15 | 华中农业大学 | Degradation method of konjac glucomannan |
| CN112280814A (en) * | 2020-10-29 | 2021-01-29 | 武汉芘芘薇莎生物科技有限公司 | High-content medium-low molecular weight konjac glucomannan, preparation method thereof and functional composition thereof |
| CN113215047A (en) * | 2021-05-20 | 2021-08-06 | 西北大学 | Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent |
| CN113215047B (en) * | 2021-05-20 | 2022-08-09 | 西北大学 | Application of konjac polysaccharide degradation products KGM-1k and KGM-5k in preparation of probiotic protective agent |
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