CN102429973A - Preparation method of traditional Chinese medicinal extract with effect of enhancing fracture healing - Google Patents
Preparation method of traditional Chinese medicinal extract with effect of enhancing fracture healing Download PDFInfo
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Abstract
The invention discloses a preparation method of a traditional Chinese medicinal extract with the effect of enhancing fracture healing. The preparation method is characterized by comprising the following steps of: after crushing of Hainan nine-section leaves, scaevola sericea and kalanchoe pinnata medicines, firstly applying distilled water for extraction with the material ratio of an extraction solvent and the medicines being 15:1, extracting for three times totally, adopting equivalent decreasing mode for each extraction time being 2 hours, 1.5 hours and 1 hour respectively; after extraction is finished, filtering medicine dregs, collecting and combining filtrate, concentrating and obtaining concentrated liquor extracted by the distilled water of the medicines; after the medicine dregs are dried, applying 95% alcohol to carry out backflow extraction, extracting for two times totally with the extraction time being 2 hours and 1.5 hours; after extraction is finished, filtering medicine dregs, collecting and combining filtrate, recovering the solvent, obtaining concentrated liquor extracted by the 95% alcohol of the medicines; and after the two types of concentrated liquor extracted are vacuum-frozen and dried for 24 hours, obtaining dry medicine powder, carrying out vacuum drying on the medicines, then weighing the obtained dry powder, and respectively calculating the extraction rate of the distilled water and the alcohol.
Description
Technical field
The present invention relates to the method for preparing of one group of Chinese medicine extract, particularly one group of method for preparing with the Chinese medicine extract that promotes the union of fracture effect.
Background technology
Psychotria hainanensis Psychotria hainanensis is a Maguireothamnus speciosus, has another name called Folium et Cacumen Psychotriae Rubrae, blue or green Clerodendrum thomsonae Balf., is long green property shrub, and shade tolerance is extremely strong, likes the environment of warm many wet sunlight.The medication of root leaf, bitter in the mouth, puckery, cool in nature, the effect of heat-clearing and toxic substances removing, detumescence and drawing out poison, expelling wind and removing dampness, promoting blood circulation and stopping pain is arranged, cure mainly flu, diphtheria, dysentery, traumatic injury and nameless gall etc.External scholar from Rubiaceae Psychotria rubra (Lour.) Poir. platymiscium PsyehotriaforstcrianaA.Gray., isolated poly indolenine alkaloid (interaction in the Psychotria rubra (Lour.) Poir. platymiscium between poly indolenine alkaloid and the nucleotide [J]. external medical plant amedica fascicle; 1990,5 (5): 230-231.).In China Hainan Island, civilian its stem of the Lis and leaf treatment fracture, traumatic injury.
Scaevola scricea Vahl. is Goodeniaceae plant plait pittosporum tobira Scaevola scricea Vahl., grows in the seashore, usually by the sea on the sand ground or on the seashore cliff.In Taiwan, all there is distribution provinces and regions such as Fujian, Guangdong, Guangxi, in Hainan Island main product in the Wenchang, ground such as Chang River, east.Its stem, leaf medication, Li nationality area is with its stem and leaf treatment osteomyelitis.
Herba Bryophylli Pinnati, the another name Herba Senecionis Chrysanthemoidis, DABUSI is Crassulaceae plant Herba Bryophylli Pinnati Bryophyllum pinnatum (L.f.) Oken.Root and Herb are used as medicine, and bitter in the mouth, acid are cold in nature.Have the hemostasis removing heat from blood, the effect of heat-clearing and toxic substances removing cures mainly haematemesis, traumatic hemorrhage, and traumatic injury, the furuncle carbuncle, mastalgia, erysipelas, ulcer is scalded, stomachache, arthralgia, throat pain, diseases such as cough due to lung-heat.Modern age, pharmacological research showed, the methanolic extract of Herba Bryophylli Pinnati leaf is inhibited to the growth of various bacteria, and water extract has analgesia, antiinflammatory, anti-diabetic isoreactivity.Li nationality area is with its Herb treatment fracture, traumatic injury in Hainan Island.Promote the union of fracture experimental study of effect also not appear in the newspapers to above three kinds of multitude's medicament extracts at present.
Summary of the invention
The purpose of this invention is to provide a kind of method for preparing with the Chinese medicine extract that promotes the union of fracture effect.
For realizing above-mentioned purpose, technical scheme of the present invention is: a kind of method for preparing with the Chinese medicine extract that promotes the union of fracture effect is provided, it is characterized in that following steps:
(1), with Psychotria hainanensis leaf or Scaevola scricea Vahl. or Herba Bryophylli Pinnati pulverizing medicinal materials, preserve subsequent usely, medicinal powder order number is 10～60 orders;
(2), medicinal powder that step (1) is obtained extracts with distilled water, filters medicinal residues, merging filtrate concentrates, and obtains medical material distilled water extraction concentrated solution, drying is calculated extraction ratio; Wherein extraction time is 1～10 time, and each extraction time is 1～10 hour, and the material ratio (mL/g) that extracts solvent and medical material is 1: 1～30: 1, and drying mode comprises water bath method, drying under reduced pressure and vacuum freeze-drying;
(3), the medicinal residues in the step (2) are dried, carry out second extraction with ethanol, filter medicinal residues, merging filtrate reclaims solvent, obtains medical material ethanol extraction concentrated solution, and drying is calculated extraction ratio; Wherein the mode of ethanol extraction comprises reflux, extract,, and cable-styled extraction and excusing from death are extracted; Concentration of alcohol is 30%～95%, preferred 95%; Extraction time is 1～10 time, and each extraction time is 1～15 hour, and the drying mode of medical material concentrated solution comprises water bath method, drying under reduced pressure and vacuum freeze-drying, and the time of medical material concentrated solution vacuum freeze-drying comprises 0～72 hour.
Preferably, in the said step (2), medicinal powder carries out extraction time with distilled water and is preferably 3 times, and 2 hours for the first time, 15. hours for the second time, 1 hour for the third time.
Preferably, in the said step (2), medicinal powder extracts with distilled water, and the material ratio (mL/g) that extracts solvent and medical material is preferably 15: 1.
Preferably, the preferred vacuum freeze-drying mode of drying mode of said step (2) Chinese crude drug concentrated solution, the time of medical material concentrated solution vacuum freeze-drying is preferably 24 hours.
Preferably, the mode of the ethanol extraction in the said step (3) is preferably reflux, extract,, and extraction time is preferably 2 times, and extraction time is preferably 2 hours for the first time, and extraction time is preferably 1.5 hours for the second time.
Preferably, the drying mode of said step (3) Chinese crude drug concentrated solution is preferably the vacuum freeze-drying mode, and the time of medical material concentrated solution vacuum freeze-drying comprises and is preferably 24 hours.
The specific embodiment
Below in conjunction with preferred embodiment the present invention is described further, but the present invention never is limited to following embodiment.
The preparation of embodiment 1 Psychotria hainanensis leaf extract
The Psychotria hainanensis leaf is pulverized, and crosses 60 mesh sieves, preserves subsequent use.
(1) Psychotria hainanensis leaf distilled water extraction test
1, the screening of extraction time: setting the material ratio (mL/g) that extracts solvent and medical material is 15: 1, extracts 2.5h at every turn, extracts respectively 1,2,3,4,5 time, to confirm suitable extraction time.
2, the screening of extraction time: setting extraction time is 3 times, and the material ratio (mL/g) that extracts solvent and medical material is 15: 1, with this understanding, extracts 0.5h, 2.5h, 3.5h, 5h, 7.5h each time respectively, and 10h is to confirm suitable each time extraction time.
3, extract the screening of the material ratio (mL/g) of solvent and medical material: setting extraction time is 3 times; Extraction time is respectively 2.0h, 1.5h, 1.0h 3 times; With this understanding; The material ratio that extracts solvent and medical material is set at 1: 1 respectively, made an experiment in 5: 1,10: 1,15: 1,20: 1,30: 1, to confirm the suitable extraction solvent and the material ratio of medical material.
4, the screening of medical material concentrated solution liquid drying mode: to carrying out drying through extracting the concentrated medical material concentrated solution that obtains, take water bath method respectively, the mode of drying under reduced pressure and vacuum freeze-drying is carried out, to confirm suitable drying mode.
5, confirming of vacuum drying time: take the mode of vacuum freeze-drying to carry out drying to extracting the concentrated medical material concentrated solution that obtains, the dry 5h of difference, 10h, 15h, 24h, 48h, 72h are to confirm suitable drying time.
(2) ethanol extraction of medicinal residues test behind the Psychotria hainanensis leaf distilled water extraction
1, the screening of ethanol extraction mode: the medicinal residues of Psychotria hainanensis leaf behind distilled water extraction dry, and adopt 95% ethanol to carry out reflux, extract,, supersound extraction and cable-styled extraction respectively, to confirm suitable extracting mode.
2, the screening of concentration of alcohol: the medicinal residues of Psychotria hainanensis leaf behind distilled water extraction dry, and adopt 30%, 60%, 70%, 80%, 95% ethanol to carry out reflux, extract, respectively, use concentration of alcohol to confirm suitable extraction.
3, the screening of extraction time: the medicinal residues of Psychotria hainanensis leaf behind distilled water extraction dry, and take 95% ethanol reflux extraction formula to extract, and extract respectively 1,2,3,4,5 time, to confirm suitable extraction time.
The screening of 4 extraction times: the medicinal residues of Psychotria hainanensis leaf behind distilled water extraction dry; Take 95% ethanol reflux extraction formula to extract, extraction time is 2 times, extracts 0.5h, 2.5h, 3.5h, 5h, 7.5h each time respectively; 10h is to confirm suitable each time extraction time.
5: the screening of medical material concentrated solution liquid drying mode: to carrying out drying through extracting the concentrated medical material concentrated solution that obtains, take water bath method respectively, the mode of drying under reduced pressure and vacuum freeze-drying is carried out, to confirm suitable drying mode.
6, confirming of vacuum drying time: take the mode of vacuum freeze-drying to carry out drying to extracting the concentrated medical material concentrated solution that obtains, the dry 5h of difference, 10h, 15h, 24h, 48h, 72h are to confirm suitable drying time.
(3) the Psychotria hainanensis leaf promotes the preparation of union of fracture extract
Take by weighing Psychotria hainanensis leaf medical material 100g, pulverize, cross 60 mesh sieves, added the 1500mL distilled water immersion 1 hour, carry out the water extraction first time then, the water extraction time is 2 hours, and extraction finishes, and filters medicinal residues, collects filtrating.Medicinal residues add the 1500mL distilled water again and carry out the water extraction second time, and extraction time is 1.5 hours, and extraction finishes, and filter medicinal residues, collect filtrating.Medicinal residues add the 1500mL distilled water again and proceed water extraction for the third time, and extraction time is 1 hour, and extraction finishes, and filter medicinal residues, collect filtrating.Merge three times the water extract, concentrate, obtain water extracting liquid.Water extracting liquid, is weighed to freeze-dried powder after 24 hours through vacuum freeze-drying, calculates extraction ratio.The result obtains the 12.5g dried powder approximately, and the distilled water extraction rate of Psychotria hainanensis leaf is about 12.5%.Psychotria hainanensis leaf medicinal residues dry behind distilled water extraction naturally, carry out alcohol reflux.Add about 10 times of amount 95% ethanol and carry out the reflux, extract, first time, extraction time is 2 hours, filters medicinal residues, collects filtrating.Medicinal residues continue to add about 10 times of amount 95% ethanol and carry out the reflux, extract, second time, and extraction time is 1.5 hours, filters medicinal residues, collects filtrating.Merge ethanol extract twice, reclaim solvent, obtain 95% ethanol extraction extractum of Psychotria hainanensis leaf, vacuum freeze-drying 24 hours is weighed to freeze-dried powder, calculates extraction ratio.The result obtains the 3.7g dried powder approximately, and 95% ethanol extraction rate of Psychotria hainanensis leaf is about 3.7%.
The preparation of embodiment two Scaevola scricea Vahl. extracts
Take by weighing Scaevola scricea Vahl. medical material 100g, pulverize, cross 60 mesh sieves, added the 1500mL distilled water immersion 1 hour, carry out the water extraction first time then, the water extraction time is 2 hours, and extraction finishes, and filters medicinal residues, collects filtrating.Medicinal residues add the 1500mL distilled water again and carry out the water extraction second time, and extraction time is 1.5 hours, and extraction finishes, and filter medicinal residues, collect filtrating.Medicinal residues add the 1500mL distilled water again and proceed water extraction for the third time, and extraction time is 1 hour, and extraction finishes, and filter medicinal residues, collect filtrating.Merge three times the water extract, concentrate, obtain water extracting liquid.Water extracting liquid, is weighed to freeze-dried powder after 24 hours through vacuum freeze-drying, calculates extraction ratio.The result obtains the 12.5g dried powder approximately, and the distilled water extraction rate of Scaevola scricea Vahl. is about 12.5%.The Scaevola scricea Vahl. medicinal residues dry behind distilled water extraction naturally, carry out alcohol reflux.Add about 10 times of amount 95% ethanol and carry out the reflux, extract, first time, extraction time is 2 hours, filters medicinal residues, collects filtrating.Medicinal residues continue to add about 10 times of amount 95% ethanol and carry out the reflux, extract, second time, and extraction time is 1.5 hours, filters medicinal residues, collects filtrating.Merge ethanol extract twice, reclaim solvent, obtain 95% ethanol extraction extractum of Scaevola scricea Vahl., vacuum freeze-drying 24 hours is weighed to freeze-dried powder, calculates extraction ratio.The result obtains the 3.7g dried powder approximately, and 95% ethanol extraction rate of Scaevola scricea Vahl. is about 3.7%.
The preparation of embodiment three Herba Bryophylli Pinnati extracts
Take by weighing Herba Bryophylli Pinnati medical material 100g, pulverize, cross 60 mesh sieves, added the 1500mL distilled water immersion 1 hour, carry out the water extraction first time then, the water extraction time is 2 hours, and extraction finishes, and filters medicinal residues, collects filtrating.Medicinal residues add the 1500mL distilled water again and carry out the water extraction second time, and extraction time is 1.5 hours, and extraction finishes, and filter medicinal residues, collect filtrating.Medicinal residues add the 1500mL distilled water again and proceed water extraction for the third time, and extraction time is 1 hour, and extraction finishes, and filter medicinal residues, collect filtrating.Merge three times the water extract, concentrate, obtain water extracting liquid.Water extracting liquid, is weighed to freeze-dried powder after 24 hours through vacuum freeze-drying, calculates extraction ratio.The result obtains the 12.5g dried powder approximately, and the distilled water extraction rate of Herba Bryophylli Pinnati is about 12.5%.The Herba Bryophylli Pinnati medicinal residues dry behind distilled water extraction naturally, carry out alcohol reflux.Add about 10 times of amount 95% ethanol and carry out the reflux, extract, first time, extraction time is 2 hours, filters medicinal residues, collects filtrating.Medicinal residues continue to add about 10 times of amount 95% ethanol and carry out the reflux, extract, second time, and extraction time is 1.5 hours, filters medicinal residues, collects filtrating.Merge ethanol extract twice, reclaim solvent, obtain 95% ethanol extraction extractum of Herba Bryophylli Pinnati, vacuum freeze-drying 24 hours is weighed to freeze-dried powder, calculates extraction ratio.The result obtains the 3.7g dried powder approximately, and 95% ethanol extraction rate of Herba Bryophylli Pinnati is about 3.7%.
Above disclosedly be merely preferred embodiment of the present invention, can not limit the present invention's interest field certainly with this, the equivalent variations of therefore doing according to claim of the present invention still belongs to the scope that the present invention is contained.
Claims (6)
1. method for preparing with the Chinese medicine extract that promotes the union of fracture effect is characterized in that following steps:
(1), with Psychotria hainanensis leaf or Scaevola scricea Vahl. or Herba Bryophylli Pinnati pulverizing medicinal materials, preserve subsequent usely, medicinal powder order number is 10～60 orders;
(2), medicinal powder that step (1) is obtained extracts with distilled water, filters medicinal residues, merging filtrate concentrates, and obtains medical material distilled water extraction concentrated solution, drying is calculated extraction ratio; Wherein extraction time is 1～10 time, and each extraction time is 1～10 hour, and the material ratio (mL/g) that extracts solvent and medical material is 1: 1～30: 1, and drying mode comprises water bath method, drying under reduced pressure and vacuum freeze-drying;
(3), the medicinal residues in the step (2) are dried, carry out second extraction with ethanol, filter medicinal residues, merging filtrate reclaims solvent, obtains medical material ethanol extraction concentrated solution, and drying is calculated extraction ratio; Wherein the mode of ethanol extraction comprises reflux, extract,, and cable-styled extraction and excusing from death are extracted; Concentration of alcohol is 30%～95%, preferred 95%; Extraction time is 1～10 time, and each extraction time is 1～15 hour, and the drying mode of medical material concentrated solution comprises water bath method, drying under reduced pressure and vacuum freeze-drying, and the time of medical material concentrated solution vacuum freeze-drying comprises 0～72 hour.
2. the method for preparing with the Chinese medicine extract that promotes the union of fracture effect as claimed in claim 1; It is characterized in that: in the said step (2), medicinal powder carries out extraction time with distilled water and is preferably 3 times, 2 hours for the first time; 15. hours for the second time, 1 hour for the third time.
3. the method for preparing with the Chinese medicine extract that promotes the union of fracture effect as claimed in claim 1, it is characterized in that: in the said step (2), medicinal powder extracts with distilled water, and the material ratio (mL/g) that extracts solvent and medical material is preferably 15: 1.
4. the method for preparing with the Chinese medicine extract that promotes the union of fracture effect as claimed in claim 1; It is characterized in that: the preferred vacuum freeze-drying mode of drying mode of said step (2) Chinese crude drug concentrated solution, the time of medical material concentrated solution vacuum freeze-drying is preferably 24 hours.
5. the method for preparing with the Chinese medicine extract that promotes the union of fracture effect as claimed in claim 1; It is characterized in that: the mode of the ethanol extraction in the said step (3) is preferably reflux, extract; Extraction time is preferably 2 times; Extraction time is preferably 2 hours for the first time, and extraction time is preferably 1.5 hours for the second time.
6. the method for preparing with the Chinese medicine extract that promotes the union of fracture effect as claimed in claim 1; It is characterized in that: the drying mode of said step (3) Chinese crude drug concentrated solution is preferably the vacuum freeze-drying mode, and the time of medical material concentrated solution vacuum freeze-drying comprises and is preferably 24 hours.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105596567A (en) * | 2015-12-31 | 2016-05-25 | 丁伟杰 | Traditional Chinese medicine composition for treating bone fracture and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1679695A (en) * | 2005-02-04 | 2005-10-12 | 张海峰 | Chinese medicine preparation for treating bone fracture and preparing method thereof |
| CN1682823A (en) * | 2005-02-25 | 2005-10-19 | 张海峰 | Compound drynaria rhizome preparation and its preparing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1679695A (en) * | 2005-02-04 | 2005-10-12 | 张海峰 | Chinese medicine preparation for treating bone fracture and preparing method thereof |
| CN1682823A (en) * | 2005-02-25 | 2005-10-19 | 张海峰 | Compound drynaria rhizome preparation and its preparing method |
Non-Patent Citations (1)
| Title |
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| 李洪福等: "海南九节叶的化学成分", 《中国实验方剂学杂志》, vol. 17, no. 19, 31 October 2011 (2011-10-31), pages 125 - 127 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105596567A (en) * | 2015-12-31 | 2016-05-25 | 丁伟杰 | Traditional Chinese medicine composition for treating bone fracture and preparation method thereof |
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