CN102423070A - Natto capsule and its preparation method - Google Patents
Natto capsule and its preparation method Download PDFInfo
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- CN102423070A CN102423070A CN2011103088453A CN201110308845A CN102423070A CN 102423070 A CN102423070 A CN 102423070A CN 2011103088453 A CN2011103088453 A CN 2011103088453A CN 201110308845 A CN201110308845 A CN 201110308845A CN 102423070 A CN102423070 A CN 102423070A
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Abstract
A natto soft capsule disclosed in the invention contains the following components of: a natto extract product, a haw extract product, soybean salad oil, phosphatidylcholine bilayer and vitamin E. A natto hard capsule disclosed in the invention contains the following components of: a natto powder, a haw extract product, a matrimony vine extract product, an oriental waterplantain rhizome extract product and starch. A preparation method comprises steps of: natto active matter extraction, haw extract product preparation, natto powder preparation, matrimony vine extract product preparation, oriental waterplantain rhizome extract product preparation and capsule formation. The natto capsule has a reasonable formula, is convenient to take and has a remarkable food therapy value and a therapeutic effect, and has a good health-care and body-strengthening effect. Therefore, by the method of preparing into soft capsules and extracting active matter, strong ammonia smell of natto is covered, and its bioavailability can be also raised.
Description
Technical field:
The present invention relates to health product technology field, be specially a kind of natto soft capsule and a kind of natto hard shell capsules and preparation method.
Background technology:
Natto is a kind of traditional food of Japan, is a kind of functional food of being processed by hay bacillus (Bacillus subtilis) or Bacillus natto (Bacillusnatto) fermentation boiling soybean, the edible history in existing more than 2000 year.Natto contains physiological activators such as some Nattokinases, isoflavones, lecithin, superoxide dismutase (SOD), unrighted acid and vitamin E; Has plurality of health care functions; Often edible natto not only can effectively prevent and treat cardiovascular and cerebrovascular disease, also have anticancer, prevent and treat osteoporosis, regulate the enteron aisle balance, effect such as anti-oxidant.In recent years; China's ageing phenomenon is more and more serious, and the incidence of disease of cerebrovascular system disease, cancer etc. constantly raises, because natto has preferably thrombus dissolving, effect such as hypotensive and antitumor; Therefore develop natto food, can bring good economic benefit and social benefit.Though yet natto itself has many health cares, traditional Japanese Natto has the ammonia stink, and its local flavor is difficult to Chinese and accepts; Storage life is short in addition, product is single; Can not make its bigizationner of effect, through in natto, adding some Chinese herbal medicines, through rational formulating; Can strengthen some particular functionality, play preferably the effect that prevents, keeps healthy and treat.
Before the application proposes; Existing people proposed similar patent application to State Patent Office; But owing to mainly be in the prescription as primary raw material, in process, through not being that very appropriate process is handled with natto powder or natto extract; The active ingredient loss is very big, is difficult to the effect that reaches desirable.And the ammonia of natto flavor itself is difficult to eliminate, and the consumer does not take like a shot when edible.Processing technology also is to adopt traditional simple method in addition, is regardless of material characteristic, pulverizes all, mixing, preparation.The natto product bioavilability of processing is not high, and prevention, health care and result of treatment are not good.
Summary of the invention:
The technical problem that the present invention will solve provides a kind of natto soft capsule and a kind of natto hard shell capsules, with and the method for preparation, guarantee that the bioavilability of product of natto is high, prevention, health care and result of treatment are good, and mouthfeel is easy to the consumer and accepts.
The hard shell capsules that the soft capsule of being processed by natto extract, haw thorn extract, soybean salad oil, lecithin, vitamin E that the present invention proposes and natto powder, haw thorn extract, wolfberry fruit extract, Alisma extract, starch are processed.Its prescription is more reasonable, adopts production technology more can keep the active ingredient in the natto, and the extraction in addition special to wherein specific medicinal material, obtains required active ingredient, makes prepared product have more specific effect.
For the scheme that solves the problems of the technologies described above employing is:
A kind of natto soft capsule comprises that following composition constitutes: natto extract, haw thorn extract, soybean salad oil, lecithin, vitamin E.
As preferably, comprise that by mass percentage following composition constitutes: natto extract 23%, haw thorn extract 16%, soybean salad oil 59%, soybean lecithin 1.9%, vitamin E 0.1%.
The natto soft capsule preparation comprises following operation:
The extraction of A, natto active matter: the SPSS with 1 times (V/W) under aseptic, cryogenic conditions joins in the fresh natto (without after-ripening technology); And stir 30min gently with glass bar; Make the abundant wash-out of natto active principle; And, obtain the eluent of the active component of natto with the sterile gauze filtration.With ascorbic acid 2 times, mix by 1: 1 (V/V) with the natto eluent after the degerming, with regulating pH value to 7.0～7.2 behind the eddy mixer mixing with distilled water diluting to desired concn; Be sub-packed in the freeze-drying bottle; Thickness is about 10mm, places 8 ℃ of refrigerator pre-freeze 6h, puts into freeze dryer then; At condenser temperature is-45 ℃, and vacuum is that 13.3Pa, heating shelf temperature are under the condition about 30 ℃ sample to be carried out freeze drying 16h.After drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve places closed container, 4 ℃ of refrigerations are subsequent use;
The preparation of B, haw thorn extract: it is subsequent use to get the hawthorn that selects after the arrangement, gets the hawthorn medicinal material and removes nuclear, adds 8 times of amount 60% alcohol refluxs and extracts 3 times, at every turn 2h; Reclaim ethanol, to 1: 1 concentration of medicinal material, with water saturation extracting n-butyl alcohol 3 times; Evaporate to dryness, with 3 times medicinal material amount 60% dissolve with ethanol, its solution is refining with macroporous absorbent resin; Get the solution concentration after making with extra care, concentrate is carried out freeze drying, subsequent use;
Again, get the hawthorn that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder, adds 10 times of amount 75% ethanol; Backflow 3h filters, and reclaims ethanol, and residue is with 10 times of amount ethyl acetate backflow 3h; Filter, reclaim ethyl acetate, concentrate is carried out freeze drying, subsequent use;
The haw thorn extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use.
C, soft capsule preparation:
Press formula ratio, gelatin: water: glycerine=1: 1: 0.4, water intaking adds 0.15% oxybenzene ester; Behind the 1.5% titanium dioxide mixing, mix, add gelatin dipping 2h, in 70-80 ℃ of water-bath, be heated to dissolving with glycerine; The companion is stirred in 60 ℃ of insulations, the airtight glue jar of joining, and the open vacuum pump vacuumizes, and purifies to the glue bubble approximately; Till gelatin all dissolves,, subsequent use through 80 order nylon net filters;
Get first soybean salad oil, soybean lecithin, vitamin E by prescription and in colloid mill, grind, it is mixed, add natto extract, haw thorn extract then while grinding, it is mixed, and transfer pH value subsequent use between 6-7;
The material utilizations rotation mould legal systems that two kinds are got ready are equipped with soft capsule, and in the preparation process, and two kinds of materials otherwise the companion is stirred in the circulation that stops make its every material soft capsule quality stable.
A kind of natto hard shell capsules comprises that following composition constitutes: natto powder, haw thorn extract, wolfberry fruit extract, Alisma extract, starch.
As preferably, the following composition of quality percentage constitutes: natto powder 25.5%, haw thorn extract 25.5%, wolfberry fruit extract 19%, Alisma extract 17%, starch 13%.
Preparation natto hard shell capsules comprises following operation:
(1), natto powder preparation: the soya bean of getting after selected was soaked in water 10-24 hour; Make it fully steep out the back under temperature more than 100 ℃ boiling 20-60 minute; When the ripe beans after the boiling are cooled to 20-40 ℃; Evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation.To ferment, the natto that produces more transparent haircuts places and refrigerates in the 0-I0 ℃ of refrigerating chamber more than 24 hours, takes out; Carry out freeze drying, in aseptic condition descends with the deep cooling pulverizer,, cross the 40-80 mesh sieve 0-8 ℃ of pulverizing; Place closed container, 4 ℃ of refrigerations are subsequent use;
(2), haw thorn extract preparation: method is the same;
(3), wolfberry fruit extract preparation: get the matrimony vine that selects after the arrangement, under 60 ℃, carry out drying, crushed after being dried written treaty 10 purpose powder; 60% ethanol of the 10 times of amounts of powder adding after the pulverizing carries out constant temperature in 35 ℃ and soaks 24h, filters, and decompression filtrate recycling ethanol also concentrates; Concentrate is carried out freeze drying, after drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing; Cross the 40-80 mesh sieve, place closed container, refrigerate subsequent use
(4), Alisma extract preparation: get the rhizoma alismatis that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder; The benzinum that powder after the pulverizing adds 2 times of amounts soaks; After soaking its powder is carried out suction filtration and get meal, and meal is dried in the shade, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis water extract, adds the distillation decocting 3 times, adds water doubly at every turn, filters after decocting 1h, collects filtrating, and is centrifugal, get supernatant with 2000 commentariess on classics/min, stand at low temperature spend the night the back filter, concentrated, concentrate is carried out freeze drying, subsequent use;
The preparation of rhizoma alismatis alcohol extract is got the rhizoma alismatis meal and is added 80% alcohol reflux extraction 3 times, adds 6 times of ethanol at every turn; Filter behind the refluxing extraction 2h for the first time; Second and third time filtered behind the refluxing extraction 1h respectively, and filtrating is with 2000 commentaries on classics/min, and be centrifugal, get supernatant concentration; Concentrate is carried out freeze drying, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis polysaccharide, extracts 2 times with 80% alcohol reflux, adds 6 times of amount ethanol for the first time, and refluxing extraction 3h filters; 4 times of amounts for the second time, 2h filters, and with 50 ℃ of dryings of the dregs of a decoction, adds 7 times of distilled water of meal amount in 90 ℃ of warm lixiviate 3h, filters; The dregs of a decoction are got 2h with 3 times of distilled water of meal amount in 90 ℃ of warm lixiviates again, filter, and repeat once merging filtrate with 2 times of distilled water of meal amount again; Being evaporated to volume is 2 times of meal amounts, with 3000 commentaries on classics/min, and centrifugal removal deposition, supernatant; Filter, add 95% ethanol of 5 times of amounts in the filtrating, stand at low temperature is spent the night, and the deposition polysaccharide also filters; Filter cake repeatedly washs with absolute ethyl alcohol, acetone, ether successively, 50 ℃ of dryings, and it is subsequent use to get the thick polysaccharide of rhizoma alismatis;
The Alisma extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use;
(6), hard shell capsules preparation:
The preparation of natto hard shell capsules is after the natto powder of recipe quantity, haw thorn extract, wolfberry fruit extract, Alisma extract, starch are mixed, to carry out filling with capsule, promptly gets.
Good effect:
Natto: be one of japanese traditional fermented food; Some kinds or natto bud pole bacterium (Bac river us natto) fermentation soybean by withered grass bud pole bacterium (Bacillus Subtilis) are processed H1; Contain multiple materials such as protease, multivitamin, Y one glutamyl transpeptidase, Y one polyglutamic acid in the natto; Also contain simultaneously higher active material; Shout like Nattokinase, isoflavones, superoxide dismutase (SOD), saponin etc. have anti-oxidant Yin, hypotensive n thrombus dissolving team is antitumor ' 91, prevent multiple functions such as osteoporosis, short blood coagulation, and is commonly used with prevention and treatment cardiac and cerebral vascular diseases.
Hawthorn: contain multivitamin, crataegolic acid, tartaric acid, citric acid, malic acid etc., also contain mineral matters such as flavonoids, lactone, carbohydrate, protein, fat and calcium, phosphorus, iron, contained lipolytic enzyme can promote the digestion of fat food.Promote functions such as gastric secretion and increase stomach endoenzyme element.Contain drug ingedients such as mountain terpene and flavonoids in the hawthorn, have significant hemangiectasis and hypotensive effect, the function that strengthens cardiac muscle, arrhythmia, adjusting blood fat and cholesterol level is arranged.
Rhizoma alismatis: stem tuber contains and rushes down pure A, B, C, alisol A monoacetate; The alisol B monoacetate, the single acetate vinegar of alisol C epialisol A, rhizoma alismatis Azulene alcohol; Rhizoma alismatis Azulene alcohol oxide; 16 'beta '-methoxy alisol B monoacetates, 16 beta-hydroxy alisol B monoacetates, sitosterol-3-O-stearyl-β-D-glucopyanoside.Also contain choline (cho-line), elements such as sugar and potassium, calcium, magnesium.Modern medicine study, rhizoma alismatis can reduce serum total cholesterol and triacylglycerol content, slow down atherosclerotic and form; Rhizoma alismatis and preparation modern times thereof also are used to treat auditory vertigo, dyslipidemia, seminal emission, fatty liver and diabetes etc.
The fruit of Chinese wolfberry: be liver-kidney tonifying Chinese medicine commonly used simply, its look scarlet, and its flavor is fragrant and sweet.Modern medicine study confirms that it contains nutritional labelings such as betaine, polysaccharide, crude fat, crude protein, carrotene, vitamin A, vitamin C, vitamin B1, vitamin B2 and calcium, phosphorus, iron, zinc, manganese, linoleic acid; Hematopoiesis function there is facilitation, can also be anti-ageing, anti-sudden change, antitumor, AFL and effect such as hypoglycemic.The traditional Chinese medical science usually with it treat liver-kidney Yin deficiency, soreness and weakness of waist and knees, dizziness, forgetful, dizzy, many tears of blurred vision, quench one's thirst, illness such as seminal emission.The effect with uniqueness of the fruit of Chinese wolfberry aspect enhancing sexual function,
Reasonable recipe of the present invention, taking convenience, dietotherapy, medication effect are remarkable.Good health body-building effect is arranged.And Chinese medicine is all the article of medicine-food two-purpose in the side, and is without any side effects, is applicable to all kinds of crowds.In the soft capsule prescription, hawthorn has significant hemangiectasis and hypotensive effect, and the function that strengthens cardiac muscle, arrhythmia, adjusting blood fat and cholesterol level is arranged.The anti-hypotensive n thrombus dissolving of natto extract team is antitumor ' 91, prevent multiple functions such as osteoporosis, short blood coagulation, and the two matches, and effect supplements and complements each other, and takes throughout the year, can well prevent to alleviate cardiovascular and cerebrovascular disease, promote longevity.Hard shell capsules prescription is with natto, hawthorn, and rhizoma alismatis is main, is aided with matrimony vine, and several persons close and join, and bring out the best in each other, hypoglycemic, blood fat, and anti-ageing, anti-sudden change, antineoplastic action are obvious.
Among the present invention, because of the strong ammonia flavor of natto tool, and mainly act as Bacillus natto and Nattokinase wherein, thereby take to process soft capsule and the method for extracting its active matter, both that natto is strong ammonia flavor covers, and can improve the effect of its bioavilability again.
The size of feed particles is the key factor that influences the product dissolution rate, and dissolution rate directly influences the health-care effect of goods.Show that through test when feedstock particle size was the 40-80 order, dissolution rate was the highest, therefore, the raw material granularity of confirming in this technology is 20 orders-40 orders.Natto of using among the present invention and natto extract, and are avoided and the contacting of copper, zinc, aluminium etc. without high-temperature process because of in process of production, so to its contained active component is not had destruction.
The specific embodiment:
Embodiment 1, natto soft capsule and preparation method thereof:
The natto soft capsule comprises that according to mass percent following composition constitutes: natto extract 23%, haw thorn extract 16%, soybean salad oil 59%, soybean lecithin 1.9%, vitamin E 0.1%
The preparation method
The extraction of A, natto active matter: the SPSS with 1 times (V/W) under aseptic, cryogenic conditions joins in the fresh natto (without after-ripening technology); And stir 30min gently with glass bar; Make the abundant wash-out of natto active principle; And, obtain the eluent of the active component of natto with the sterile gauze filtration.With ascorbic acid 2 times, mix by 1: 1 (V/V) with the natto eluent after the degerming, with regulating pH value to 7.0～7.2 behind the eddy mixer mixing with distilled water diluting to desired concn; Be sub-packed in the freeze-drying bottle; Thickness is about 10mm, places 8 ℃ of refrigerator pre-freeze 6h, puts into freeze dryer then; At condenser temperature is-45 ℃, and vacuum is that 13.3Pa, heating shelf temperature are under the condition about 30 ℃ sample to be carried out freeze drying 16h.After drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve places closed container, 4 ℃ of refrigerations are subsequent use;
The preparation of B, haw thorn extract: it is subsequent use to get the hawthorn that selects after the arrangement, gets the hawthorn medicinal material and removes nuclear, adds 8 times of amount 60% alcohol refluxs and extracts 3 times, at every turn 2h; Reclaim ethanol, to 1: 1 concentration of medicinal material, with water saturation extracting n-butyl alcohol 3 times; Evaporate to dryness, with 3 times medicinal material amount 60% dissolve with ethanol, its solution is refining with macroporous absorbent resin; Get the solution concentration after making with extra care, concentrate is carried out freeze drying, subsequent use;
Again, get the hawthorn that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder, adds 10 times of amount 75% ethanol; Backflow 3h filters, and reclaims ethanol, and residue is with 10 times of amount ethyl acetate backflow 3h; Filter, reclaim ethyl acetate, concentrate is carried out freeze drying, subsequent use;
The haw thorn extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use.
C, soft capsule preparation:
Press formula ratio, gelatin: water: glycerine=1: 1: 0.4, water intaking adds 0.15% oxybenzene ester; Behind the 1.5% titanium dioxide mixing, mix, add gelatin dipping 2h, in 70-80 ℃ of water-bath, be heated to dissolving with glycerine; The companion is stirred in 60 ℃ of insulations, the airtight glue jar of joining, and the open vacuum pump vacuumizes, and purifies to the glue bubble approximately; Till gelatin all dissolves,, subsequent use through 80 order nylon net filters;
Get first soybean salad oil, soybean lecithin, vitamin E by prescription and in colloid mill, grind, it is mixed, add natto extract, haw thorn extract then while grinding, it is mixed, and transfer pH value subsequent use between 6-7;
The material utilizations rotation mould legal systems that two kinds are got ready are equipped with soft capsule, and in the preparation process, and two kinds of materials otherwise the companion is stirred in the circulation that stops make its every material soft capsule quality stable.
Examples of implementation 2
Natto hard shell capsules and preparation method thereof
The natto hard shell capsules constitutes by the following composition of quality percentage: natto powder 25.5%, haw thorn extract 25.5%, wolfberry fruit extract 19%, Alisma extract 17%, starch 13%.
Preparation natto hard shell capsules comprises following operation:
(1), natto powder preparation: the soya bean of getting after selected was soaked in water 10-24 hour; Make it fully steep out the back under temperature more than 100 ℃ boiling 20-60 minute; When the ripe beans after the boiling are cooled to 20-40 ℃; Evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation.To ferment, the natto that produces more transparent haircuts places and refrigerates in the 0-I0 ℃ of refrigerating chamber more than 24 hours, takes out; Carry out freeze drying, in aseptic condition descends with the deep cooling pulverizer,, cross the 40-80 mesh sieve 0-8 ℃ of pulverizing; Place closed container, 4 ℃ of refrigerations are subsequent use;
(2), haw thorn extract preparation: method is the same;
(3), wolfberry fruit extract preparation: get the matrimony vine that selects after the arrangement, under 60 ℃, carry out drying, crushed after being dried written treaty 10 purpose powder; 60% ethanol of the 10 times of amounts of powder adding after the pulverizing carries out constant temperature in 35 ℃ and soaks 24h, filters, and decompression filtrate recycling ethanol also concentrates; Concentrate is carried out freeze drying, after drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing; Cross the 40-80 mesh sieve, place closed container, refrigerate subsequent use
(4), Alisma extract preparation: get the rhizoma alismatis that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder; The benzinum that powder after the pulverizing adds 2 times of amounts soaks; After soaking its powder is carried out suction filtration and get meal, and meal is dried in the shade, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis water extract, adds the distillation decocting 3 times, adds water doubly at every turn, filters after decocting 1h, collects filtrating, and is centrifugal, get supernatant with 2000 commentariess on classics/min, stand at low temperature spend the night the back filter, concentrated, concentrate is carried out freeze drying, subsequent use;
The preparation of rhizoma alismatis alcohol extract is got the rhizoma alismatis meal and is added 80% alcohol reflux extraction 3 times, adds 6 times of ethanol at every turn; Filter behind the refluxing extraction 2h for the first time; Second and third time filtered behind the refluxing extraction 1h respectively, and filtrating is with 2000 commentaries on classics/min, and be centrifugal, get supernatant concentration; Concentrate is carried out freeze drying, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis polysaccharide, extracts 2 times with 80% alcohol reflux, adds 6 times of amount ethanol for the first time, and refluxing extraction 3h filters; 4 times of amounts for the second time, 2h filters, and with 50 ℃ of dryings of the dregs of a decoction, adds 7 times of distilled water of meal amount in 90 ℃ of warm lixiviate 3h, filters; The dregs of a decoction are got 2h with 3 times of distilled water of meal amount in 90 ℃ of warm lixiviates again, filter, and repeat once merging filtrate with 2 times of distilled water of meal amount again; Being evaporated to volume is 2 times of meal amounts, with 3000 commentaries on classics/min, and centrifugal removal deposition, supernatant; Filter, add 95% ethanol of 5 times of amounts in the filtrating, stand at low temperature is spent the night, and the deposition polysaccharide also filters; Filter cake repeatedly washs with absolute ethyl alcohol, acetone, ether successively, 50 ℃ of dryings, and it is subsequent use to get the thick polysaccharide of rhizoma alismatis;
The Alisma extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use;
(6), hard shell capsules preparation:
The preparation of natto hard shell capsules is after the natto powder of recipe quantity, haw thorn extract, wolfberry fruit extract, Alisma extract, starch are mixed, to carry out filling with capsule, promptly gets
Claims (6)
1. a natto soft capsule is characterized in that, comprises that following composition constitutes: natto extract, haw thorn extract, soybean salad oil, lecithin, vitamin E.
2. a kind of bean-containing capsule as claimed in claim 1 is characterized in that, comprises that by mass percentage following composition constitutes: natto extract 23%, haw thorn extract 16%, soybean salad oil 59%, soybean lecithin 1.9%, vitamin E 0.1%.
3. preparation natto soft capsule according to claim 1 or claim 2 is characterized in that, comprises following operation:
The extraction of A, natto active matter: the SPSS with 1 times (V/W) under aseptic, cryogenic conditions joins in the fresh natto (without after-ripening technology); And stir 30min gently with glass bar; Make the abundant wash-out of natto active principle; And, obtain the eluent of the active component of natto with the sterile gauze filtration.With ascorbic acid 2 times, mix by 1: 1 (V/V) with the natto eluent after the degerming, with regulating pH value to 7.0～7.2 behind the eddy mixer mixing with distilled water diluting to desired concn; Be sub-packed in the freeze-drying bottle; Thickness is about 10mm, places 8 ℃ of refrigerator pre-freeze 6h, puts into freeze dryer then; At condenser temperature is-45 ℃, and vacuum is that 13.3Pa, heating shelf temperature are under the condition about 30 ℃ sample to be carried out freeze drying 16h.After drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve places closed container, 4 ℃ of refrigerations are subsequent use;
The preparation of B, haw thorn extract: it is subsequent use to get the hawthorn that selects after the arrangement, gets the hawthorn medicinal material and removes nuclear, adds 8 times of amount 60% alcohol refluxs and extracts 3 times, at every turn 2h; Reclaim ethanol, to 1: 1 concentration of medicinal material, with water saturation extracting n-butyl alcohol 3 times; Evaporate to dryness, with 3 times medicinal material amount 60% dissolve with ethanol, its solution is refining with macroporous absorbent resin; Get the solution concentration after making with extra care, concentrate is carried out freeze drying, subsequent use;
Again, get the hawthorn that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder, adds 10 times of amount 75% ethanol; Backflow 3h filters, and reclaims ethanol, and residue is with 10 times of amount ethyl acetate backflow 3h; Filter, reclaim ethyl acetate, concentrate is carried out freeze drying, subsequent use;
The haw thorn extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use.
C, soft capsule preparation:
Press formula ratio, gelatin: water: glycerine=1: 1: 0.4, water intaking adds 0.15% oxybenzene ester; Behind the 1.5% titanium dioxide mixing, mix, add gelatin dipping 2h, in 70-80 ℃ of water-bath, be heated to dissolving with glycerine; The companion is stirred in 60 ℃ of insulations, the airtight glue jar of joining, and the open vacuum pump vacuumizes, and purifies to the glue bubble approximately; Till gelatin all dissolves,, subsequent use through 80 order nylon net filters;
Get first soybean salad oil, soybean lecithin, vitamin E by prescription and in colloid mill, grind, it is mixed, add natto extract, haw thorn extract then while grinding, it is mixed, and transfer pH value subsequent use between 6-7;
The material utilizations rotation mould legal systems that two kinds are got ready are equipped with soft capsule, and in the preparation process, and two kinds of materials otherwise the companion is stirred in the circulation that stops make its every material soft capsule quality stable.
4. a natto hard shell capsules is characterized in that, comprises that following composition constitutes: natto powder, haw thorn extract, wolfberry fruit extract, Alisma extract, starch.
5. natto hard shell capsules as claimed in claim 4 is characterized in that, comprises that by mass percentage following composition constitutes: natto powder 25.5%, haw thorn extract 25.5%, wolfberry fruit extract 19%, Alisma extract 17%, starch 13%.
6. preparation is characterized in that comprising following operation like claim 4 or 5 described natto hard shell capsules:
(1), natto powder preparation: the soya bean of getting after selected was soaked in water 10-24 hour; Make it fully steep out the back under temperature more than 100 ℃ boiling 20-60 minute; When the ripe beans after the boiling are cooled to 20-40 ℃; Evenly spray into Bacillus natto liquid, cultivated 18-30 hour at temperature 35-45 ℃, relative humidity 50-90% condition bottom fermentation.To ferment, the natto that produces more transparent haircuts places and refrigerates in the 0-I0 ℃ of refrigerating chamber more than 24 hours, takes out; Carry out freeze drying, in aseptic condition descends with the deep cooling pulverizer,, cross the 40-80 mesh sieve 0-8 ℃ of pulverizing; Place closed container, 4 ℃ of refrigerations are subsequent use;
(2), the preparation of the haw thorn extract described in claim 3;
(3), wolfberry fruit extract preparation: get the matrimony vine that selects after the arrangement, under 60 ℃, carry out drying, crushed after being dried written treaty 10 purpose powder; 60% ethanol of the 10 times of amounts of powder adding after the pulverizing carries out constant temperature in 35 ℃ and soaks 24h, filters, and decompression filtrate recycling ethanol also concentrates; Concentrate is carried out freeze drying, after drying finishes, with the freeze-drying sample in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing; Cross the 40-80 mesh sieve, place closed container, refrigerate subsequent use
(4), Alisma extract preparation: get the rhizoma alismatis that selects after the arrangement, under 50 ℃, carry out drying, crushed after being dried becomes 20～40 purpose powder; The benzinum that powder after the pulverizing adds 2 times of amounts soaks; After soaking its powder is carried out suction filtration and get meal, and meal is dried in the shade, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis water extract, adds the distillation decocting 3 times, adds water doubly at every turn, filters after decocting 1h, collects filtrating, and is centrifugal, get supernatant with 2000 commentariess on classics/min, stand at low temperature spend the night the back filter, concentrated, concentrate is carried out freeze drying, subsequent use;
The preparation of rhizoma alismatis alcohol extract is got the rhizoma alismatis meal and is added 80% alcohol reflux extraction 3 times, adds 6 times of ethanol at every turn; Filter behind the refluxing extraction 2h for the first time; Second and third time filtered behind the refluxing extraction 1h respectively, and filtrating is with 2000 commentaries on classics/min, and be centrifugal, get supernatant concentration; Concentrate is carried out freeze drying, subsequent use;
The rhizoma alismatis meal is got in the preparation of rhizoma alismatis polysaccharide, extracts 2 times with 80% alcohol reflux, adds 6 times of amount ethanol for the first time, and refluxing extraction 3h filters; 4 times of amounts for the second time, 2h filters, and with 50 ℃ of dryings of the dregs of a decoction, adds 7 times of distilled water of meal amount in 90 ℃ of warm lixiviate 3h, filters; The dregs of a decoction are got 2h with 3 times of distilled water of meal amount in 90 ℃ of warm lixiviates again, filter, and repeat once merging filtrate with 2 times of distilled water of meal amount again; Being evaporated to volume is 2 times of meal amounts, with 3000 commentaries on classics/min, and centrifugal removal deposition, supernatant; Filter, add 95% ethanol of 5 times of amounts in the filtrating, stand at low temperature is spent the night, and the deposition polysaccharide also filters; Filter cake repeatedly washs with absolute ethyl alcohol, acetone, ether successively, 50 ℃ of dryings, and it is subsequent use to get the thick polysaccharide of rhizoma alismatis;
The Alisma extract sample that above distinct methods is extracted in aseptic condition down with the deep cooling pulverizer in 0-8 ℃ of pulverizing, mistake 40-80 mesh sieve mixes, and places closed container, refrigerates subsequent use;
(6), hard shell capsules preparation:
The preparation of natto hard shell capsules is after the natto powder of recipe quantity, haw thorn extract, wolfberry fruit extract, Alisma extract, starch are mixed, to carry out filling with capsule, promptly gets.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716471A (en) * | 2012-07-03 | 2012-10-10 | 童长虹 | Anti-hyperlipidemia health-care product |
| CN105030950A (en) * | 2015-08-06 | 2015-11-11 | 山东凤凰生物有限公司 | Hyperlipidemia preventing and treating microecological preparation and preparation method and application thereof |
| WO2015172608A1 (en) * | 2014-05-14 | 2015-11-19 | 上海春芝堂生物制品有限公司 | Capsule for assisting in reducing blood fat and preparation method therefor |
| JP7423179B2 (en) | 2017-12-28 | 2024-01-29 | 小林製薬株式会社 | Foods containing polyphenols |
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2011
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102716471A (en) * | 2012-07-03 | 2012-10-10 | 童长虹 | Anti-hyperlipidemia health-care product |
| WO2015172608A1 (en) * | 2014-05-14 | 2015-11-19 | 上海春芝堂生物制品有限公司 | Capsule for assisting in reducing blood fat and preparation method therefor |
| CN105030950A (en) * | 2015-08-06 | 2015-11-11 | 山东凤凰生物有限公司 | Hyperlipidemia preventing and treating microecological preparation and preparation method and application thereof |
| CN105030950B (en) * | 2015-08-06 | 2019-01-18 | 山东凤凰生物有限公司 | The probiotics and its preparation method and application for preventing and treating hyperlipidemia |
| JP7423179B2 (en) | 2017-12-28 | 2024-01-29 | 小林製薬株式会社 | Foods containing polyphenols |
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