CN102408497A - Pesticide-carrying nano micellar solution as well as preparation method and application thereof - Google Patents
Pesticide-carrying nano micellar solution as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a pesticide-carrying nano micellar solution as well as a preparation method and application thereof. The pesticide-carrying nano micellar solution is prepared by a method comprising the following steps: dissolving a long-chain hydrophobic group grafted carboxymethyl chitosan into water, so as to obtain a solution B; dissolving a pesticide into water or ethanol, so as to obtain a solution C; and slowly dropwise adding the solution C to the solution B, and then evaporating and removing ethanol, so as to obtain the pesticide-carrying nano micellar solution. According to the invention, the solubility of a hydrophobic pesticide can be improved and simultaneously the slow decomposition of the pesticide can be ensured, through the adoption of the long-chain group grafted carboxymethyl chitosan; after the pesticide-carrying nano micellar solution is sprayed to the surface of a plant, a film can be formed, the pesticide and plant nutrients in the film can be slowly released, thereby exerting effects of continuous insect killing, bacteriostasis, fertilizer enhancement and growth promotion on the plant.
Description
Technical field
The invention belongs to agricultural technology field; Be specifically related to a kind of amphipathic, degradable long-chain hydrophobic grouping grafting Preparation of Carboxymethylchitosan method; And medicine carrying (agricultural chemicals and/or the plant nutrient) nano micellar solution for preparing by this long-chain hydrophobic group grafting CMS; This medicament-carried nano micelle solution can help the antibacterial disease-resistant fruit retention of melon and fruit at melon and fruit or the crosslinked film forming of plant surface and sustained-release pesticides and plant nutrient.
Background technology
In recent years, food safety is paid close attention to by the whole people, shows unique characteristics, the agricultural-food of safe green receive human consumer's favor deeply.Utilize natural biologic material with low cost to combine advanced control release technic for raw material, adopt the nano biological degradation material as carrier, load agricultural chemicals and plant nutrient are cross-linked into release-controlled film at melon and fruit or plant surface non covalent bond; With long-acting slow-release agricultural chemicals or plant nutrient melon and fruit etc. is played the effect of diseases prevention fruit retention, can improve the melon and fruit outward appearance, improve the melon and fruit setting ratio; And can reduce the usage quantity of agricultural chemicals and fertilizer; Reducing pesticide residue, benefit the society, is enterprise's considerable economic benefit simultaneously.
In recent years, polymer nano micelle receives extensive concern with its particular performances, and in medical and health, biotechnology field has broad prospect of application.Polymer nano micelle forms stable shell-core nanometer structure by block polymer or graft type polymkeric substance through self aggregation usually.
Chitosan extensively is present in the shell of shrimp crab; The source is abundant; With low cost, be the surperficial positively charged natural polymer of the unique existence of occurring in nature, have good biocompatibility and biodegradability; And chitosan can produce broad spectrum resistance by inducing plant; The autoimmunity of enhancement of plant and direct inhibition various plants pathogenic micro-organism (such as blight and tobacco mosaic virus(TMV) etc.) also can be used as plant-growth regulator, significantly improve crop yield (such as commodity " biological Nike seedling " etc.).Yet chitosan can not directly be dissolved in water, has limited its application power.People have correspondingly shifted to modified chitosan with research, i.e. chitosan derivatives.
In a large amount of chitosan derivatives, CMS is proved to be a kind of water-solubility chitosan derivative with good biocompatibility, but it can not form stable micella in water.
Summary of the invention
For the shortcoming and deficiency that overcome prior art, primary and foremost purpose of the present invention is to provide the preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS).
Another object of the present invention is to provide the long-chain hydrophobic grouping grafting that is obtained by method for preparing CMS, this long-chain hydrophobic grouping grafting CMS can be dissolved in the water, and can in water, form the stabilized nano micella.
A purpose more of the present invention is to provide a kind of preparation method of medicament-carried nano micelle solution, and this medicament-carried nano micelle solution is prepared by above-mentioned long-chain hydrophobic grouping grafting CMS.
Another purpose of the present invention is to provide the medicament-carried nano micelle that is obtained by method for preparing solution.
The application of the medicament-carried nano micelle solution that the 5th purpose of the present invention is to provide above-mentioned; But this medicament-carried nano micelle solution high-efficient carrier agricultural chemicals or plant nutrient also form the non-key of building together at plant or fruit surface and combine to be cross-linked into release-controlled film; Reach the effect of long-term diseases prevention fruit retention or controlled release plant nutrient, realize the purpose that reduces agricultural chemicals or plant nutrient amount of application and frequency of administration, reduces pesticide residue.
The object of the invention is realized through following technical proposals:
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) long-chain alcohol, sodium hydroxide and cetyl trimethylammonium bromide are mixed, stir, be heated to 60-70 ℃ of fusion; Splash into epoxy chloropropane again; At 60-65 ℃ of reaction 2-4h, filter then, collect filtrating and use water washing; The drying of filtrating is dewatered, and the alkoxyl group glycidyl ether that solvent obtains long-chain is fallen in redistillation;
(2) Pottasium Hydroxide is dissolved in obtains solution A in the Virahol; CMS is joined in the solution A, add the alkoxyl group glycidyl ether of the long-chain that step (1) prepares again, 60-65 ℃ of reaction 12-24h down; Filter then; Collect filtrating and use absolute ethanol washing, drying obtains long-chain hydrophobic grouping grafting CMS;
The mol ratio of said long-chain alcohol of step (1) and sodium hydroxide is 1: 1-3, preferred 1: 2;
The mol ratio of said long-chain alcohol of step (1) and cetyl trimethylammonium bromide is 1: 0.010-0.030, preferred 1: 0.013;
The mol ratio of said long-chain alcohol of step (1) and epoxy chloropropane is 1: 1-3, preferred 1: 2;
The said long-chain alcohol of step (1) is a monohydroxy-alcohol, and its carbonatoms is 8-32;
The temperature of the said water of step (1) is 50-70 ℃, is to remove remaining cetyl trimethylammonium bromide and sodium hydroxide with the purpose of hot wash;
In the said solution A of step (2), the concentration of Pottasium Hydroxide is 0.1-0.2g/100mL;
The mass ratio of the alkoxyl group glycidyl ether of said CMS of step (2) and long-chain is 1: 1-3.5, preferred 1: 2.1;
The temperature of the said absolute ethyl alcohol of step (2) is 50-70 ℃, is to remove remaining long-chain alcohol with the purpose of hot absolute ethanol washing.
Above-mentioned long-chain hydrophobic grouping grafting CMS can be dissolved in the water, and can in water, form the stabilized nano micella.
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
Get above-mentioned long-chain hydrophobic grouping grafting CMS and be dissolved in the water, obtain solution B; The thing of getting it filled is dissolved in water or the ethanol, obtains solution C; Solvent C slowly is added dropwise in the solution B, steams then and remove ethanol (if solvent is an ethanol), obtain medicament-carried nano micelle solution;
The concentration of long-chain hydrophobic grouping grafting CMS is 1-6g/L in the said solution B;
The concentration of said solution C Chinese traditional medicine is 10g/L;
The volume ratio of said solution C and solution B is 1: 10;
Described medicine is meant agricultural chemicals, plant nutrient or plant hormone;
Contain the micella that particle diameter is 80-300nm in the above-mentioned medicament-carried nano micelle solution.
With above-mentioned medicament-carried nano micelle spray solution to plant surface; Nano-micelle can be with the crosslinked film of forming of non covalent bond after moisture evaporation; Can be wrapped in medicine in the film; As time passes, medicine (agricultural chemicals, plant nutrient or plant hormone) can slowly release, and plant is played desinsection, antibacterial, getting fat, somatotrophic effect.
Principle of the present invention is: in chitosan structure, introduce hydrophilic radical and hydrophobic grouping and can improve that it is water-soluble; Simultaneously, the formed nuclear structure of its hydrophobic grouping can wrap pharmaceutical pack to avoid medicines structure to be destroyed, and has kept the activity of medicine.The inventive method is through being incorporated into long-chain hydrophobic grouping (the alkoxyl group glycidyl ether of the long-chain of eight to 32 carbon atoms) in the carboxymethyl chitosan glycan molecule; Obtained a kind of novel amphipathic chitose derivative, it can be directly water-soluble and can in water, self-assembly forms Stability Analysis of Structures, assemble nanometer aggregate closely.This amphipathic chitose derivative is the controlled release carrier with good filming property; Behind carrying medicament or plant nutrient ingredient; Spray on melon and fruit surface or plant leaf surface; Can be after moisture evaporation with the crosslinked film forming of non covalent bond, medicine can slowly discharge in the film, and this mould material also has air permeability and good, biological safety and biodegradability.
The present invention has following advantage and effect with respect to prior art:
1, the long-chain hydrophobic grouping grafting CMS of the present invention's preparation has the little characteristics of size, thereby can recently obtain the size that different hydrophobic chain percentage of grafting is regulated long-chain hydrophobic grouping grafting CMS through the feeding intake of alkoxyl group glycidyl ether of regulating long-chain.Long-chain hydrophobic grouping grafting CMS can form stable micella in water, can also wrap up closely by the load hydrophobic drug.
2, the long-chain hydrophobic grouping grafting CMS of the present invention's preparation can improve the solubleness of dewatering medicament as a kind of safe and effective medicine carrier, combines to form film through non covalent bond; Its surface is carboxyl and amino hydrophilic layer; Drug molecule (comprising agricultural chemicals or plant nutrient etc.) can wrapped up and protect to the intermediate hydrophobic layers that hydrophobic chain is formed, and medicine is slowly decomposed under field conditions (factors), continues to discharge carrying medicament; Keep the long-acting of medicine through controlled release drug; Thereby improve the utilization ratio of medicine, reduce conventional dispenser usage quantity and dispenser frequency, it is residual at fruit and vegetable surfaces to reduce medicine.
3, the film of the long-chain hydrophobic grouping grafting CMS formation of the present invention's preparation has many-sided using value at agriculture field; Not only can form the disease-resistant pesticide action-controlling film of fruit retention by the load agricultural chemicals; Also but load fertilizer etc. is as blade face release and release control fertilizer film, or the controlled release plant growth hormones.
4, the main raw material(s) of the long-chain hydrophobic grouping grafting CMS of the present invention's preparation is a chitosan, and its source is abundant, particularly the flourishing city of coastland fishery; The waste materials such as a large amount of shrimp shell crab shells that sea-foods such as shrimp crab produce; Just can obtain chitosan through simple roughing, thus with low cost, and prepare method of the present invention; Technology is simple, is fit to scale operation.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail, but embodiment of the present invention is not limited thereto.
Embodiment 1
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) getting 27.0kg stearyl alcohol, 5.0kg sodium hydroxide and 0.8kg cetyl trimethylammonium bromide adds in the reaction kettle and stirs; 70 ℃ of heating and meltings are added dropwise to the 9.3kg epoxy chloropropane again, remain on 65 ℃ of reaction 2h; Filtration under diminished pressure; Collect filtrating and give a baby a bath on the third day after its birth time with hot water (60 ℃), add SODIUM SULPHATE ANHYDROUS 99PCT again and dewater, solvent is fallen in underpressure distillation, and to obtain faint yellow solid be the octadecyl glycidyl ether.
(2) get 0.1kg Pottasium Hydroxide and be dissolved in the 100L Virahol, obtain solution A; The 2.0kg CMS is dispersed in the solution A; Add the octadecyl glycidyl ether that 2.0kg step (1) prepares again; After being heated to 65 ℃ of reaction 24h, suction filtration, collection is filtrated and is washed several times with hot absolute ethyl alcohol (60 ℃); Drying under reduced pressure obtains long-chain hydrophobic grouping grafting CMS, and in the aqueous solution, detecting its particle diameter through laser light scattering is 107nm.
Embodiment 2
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) getting 3.2kg octanol, 8.0kg sodium hydroxide and 0.5kg cetyl trimethylammonium bromide adds in the reaction kettle and stirs; 60 ℃ of heating and meltings are added dropwise to the 18.5kg epoxy chloropropane again, remain on 60 ℃ of reaction 3h; Decompress filter; Collect filtrating and give a baby a bath on the third day after its birth time with hot water (60 ℃), add SODIUM SULPHATE ANHYDROUS 99PCT again and dewater, solvent is fallen in underpressure distillation, and to obtain faint yellow solid be eight alkyl glycidyl ethers.
(2) get 0.1kg Pottasium Hydroxide and be dissolved in the 100L Virahol, obtain solution A; The 2.0kg CMS is dispersed in the solution A; Drop into eight alkyl glycidyl ethers that 2.6kg step (1) prepares again; After being heated to 60 ℃ of reaction 12h, suction filtration, collection is filtrated and is washed several times with hot absolute ethyl alcohol (60 ℃); Vacuum-drying obtains long-chain hydrophobic grouping grafting CMS, and in the aqueous solution, detecting its particle diameter through laser light scattering is 288nm.
Embodiment 3
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) getting 27.0kg stearyl alcohol, 10.0kg sodium hydroxide and 0.4kg cetyl trimethylammonium bromide adds in the reaction kettle and stirs; 70 ℃ of heating and meltings are added dropwise to the 20.5kg epoxy chloropropane again, remain on 65 ℃ of reaction 4h; Filtration under diminished pressure; Collect filtrating and give a baby a bath on the third day after its birth time with hot water (60 ℃), add SODIUM SULPHATE ANHYDROUS 99PCT again and dewater, solvent is fallen in underpressure distillation, and to obtain faint yellow solid be the octadecyl glycidyl ether.
(2) get 0.1kg Pottasium Hydroxide and be dissolved in the 100L Virahol, obtain solution A; The 2.0kg CMS is dispersed in the solution A; Drop into the octadecyl glycidyl ether that 4.2kg step (1) prepares again; After being heated to 60 ℃ of reaction 18h, suction filtration, collection is filtrated and is washed several times with hot absolute ethyl alcohol (60 ℃); Vacuum-drying obtains long-chain hydrophobic grouping grafting CMS, and in the aqueous solution, detecting its particle diameter through laser light scattering is 161nm.
Embodiment 4
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) getting 22.0kg tetradecyl alcohol, 12.0kg sodium hydroxide and 0.5kg cetyl trimethylammonium bromide adds in the reaction kettle and stirs; 70 ℃ of heating and meltings are added dropwise to the 18.5kg epoxy chloropropane again, remain on 65 ℃ of reaction 2h; Filtration under diminished pressure; Collect filtrating and give a baby a bath on the third day after its birth time with hot water (50 ℃), add SODIUM SULPHATE ANHYDROUS 99PCT again and dewater, solvent is fallen in underpressure distillation, and to obtain faint yellow solid be the tetradecyl glycidyl ether.
(2) get 0.1kg Pottasium Hydroxide and be dissolved in the 100L Virahol, obtain solution A; The 2.0kg CMS is dispersed in the solution A; Add the tetradecyl glycidyl ether that 5kg step (1) prepares again; After being heated to 60 ℃ of reaction 24h, suction filtration, collection is filtrated and is washed several times with hot absolute ethyl alcohol (50 ℃); Vacuum-drying obtains long-chain hydrophobic grouping grafting CMS, and in the aqueous solution, detecting its particle diameter through laser light scattering is 182nm.
Embodiment 5
The preparation method of a kind of long-chain hydrophobic grouping grafting CMS (HP-CMCS) may further comprise the steps:
(1) getting 46.0g laccerol, 5.0g sodium hydroxide and 1g cetyl trimethylammonium bromide adds in the 150mL there-necked flask and places the constant temperature magnetic stirrer to stir; Heating (70 ℃) fusion is added dropwise to the 18.5g epoxy chloropropane again, remains on 65 ℃ of reaction 2h; Decompress filter; Collect filtrating and give a baby a bath on the third day after its birth time with hot water (60 ℃), add SODIUM SULPHATE ANHYDROUS 99PCT again and dewater, solvent is fallen in underpressure distillation, and to obtain faint yellow solid be the dotriacontyl glycidyl ether.
(2) get 0.2g Pottasium Hydroxide and be dissolved in the 100mL Virahol, obtain solution A; The 2.0g CMS is dispersed in the solution A; Add the dotriacontyl glycidyl ether that 7g step (1) prepares again; After being heated to 60 ℃ of reaction 24h, suction filtration, collection is filtrated and is washed several times with hot absolute ethyl alcohol (70 ℃); Vacuum-drying obtains long-chain hydrophobic grouping grafting CMS, and in the aqueous solution, detecting its particle diameter through laser light scattering is 196nm.
Embodiment 6
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
Getting the 1g Whitfield's ointment is dissolved in the 100mL ethanol; Slowly be added drop-wise to 1L concentration again and be in the aqueous solution of the long-chain hydrophobic grouping grafting CMS that the embodiment 1 of 1mg/mL prepares; Rotary evaporation is removed residual organic solvent then, obtains the nano micellar solution of carrying medicament.
Embodiment 7
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
Get the bright amine solvent of 1g rice in 100mL ethanol; Slowly be added drop-wise to 1L concentration again and be in the aqueous solution of the long-chain hydrophobic grouping grafting CMS that the embodiment 2 of 1mg/mL prepares; Rotary evaporation is removed residual organic solvent then, obtains the nano micellar solution of carrying medicament.
Embodiment 8
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
Getting the 1g matrine is dissolved in the 100mL ethanol; Slowly be added drop-wise to 1L concentration again and be in the aqueous solution of the long-chain hydrophobic grouping grafting CMS that the embodiment 3 of 1mg/mL prepares; Rotary evaporation is removed residual organic solvent then; Carry out lyophilize then and obtain the nano-micelle powder of load matrine, can directly be dissolved in water during use and spray.
Embodiment 9
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
Get the 1g nano titanium oxide and be dissolved in the 100mL water, be added to 1L concentration and be in the aqueous solution of the long-chain hydrophobic grouping grafting CMS that the embodiment 4 of 6mg/mL prepares, obtain the nano micellar solution of load plant nutrient.Carry out lyophilize then and obtain rare earth plant nutrient nano-micelle powder, can directly be dissolved in water during use and spray.
Embodiment 10
A kind of preparation method of medicament-carried nano micelle solution may further comprise the steps:
The minimum plant element is dissolved in the 100mL water; Be added to 1L concentration and be in the aqueous solution of the long-chain hydrophobic grouping grafting CMS nano-micelle that the embodiment 5 of 1mg/mL prepares; Rotary evaporation is removed residual organic solvent then, obtains the nano micellar solution of carrying medicament.Carry out lyophilize then and obtain foliage fertilizer nano-micelle powder.
Embodiment 11
Bumpkin's agricultural-food company has carried out field experiment in the mango farm to product of the present invention in the Yunfu, Guangdong Province, in the analysis that the mango disease is carried out to former years, confirms as the shot hole of mango, and anthrax and plan dish stey are sick.
Carry out blank nano-carrier (being the long-chain hydrophobic grouping grafting CMS that embodiment 1 obtains) infusion packet of the test of kinds of schemes: a. and nano-carrier load agricultural chemicals infusion packet compares in the field; B. blank nano-carrier (being the long-chain hydrophobic grouping grafting CMS that embodiment 1 obtains) and nano-carrier load agricultural chemicals soak mango respectively; C. blank nano-carrier (being the long-chain hydrophobic grouping grafting CMS that embodiment 1 obtains) and nano-carrier load agricultural chemicals soak after the mango bagging more respectively; Every group of experiment all is provided with blank and does contrast, b, and the c group is divided into high density and low concentration group again, and different agriculture chemical compounding groups are set simultaneously.
Concrete grammar (is numbering) as follows in the bracket:
A group: blank sack (F0); Nano-carrier (embodiment 1 makes) infusion packet (F1); Nano-carrier load Whitfield's ointment (embodiment 6 makes) infusion packet (F2)
B group: (1): high density group:
Nano-micelle medicine carrying solution: water=1: 2
Nano-carrier (embodiment 1 makes) (F3 (2))
Nano-carrier load Whitfield's ointment (embodiment 6 makes) (F4 (2))
Nano-carrier load matrine (embodiment 8 makes) (F5 (2))
Nano-carrier loaded with nano TiO
2(embodiment 9 makes) (F6 (2))
The bright amine (embodiment 7 makes) (F7 (2)) of nano-carrier load rice
(2): low concentration group:
Nano-micelle medicine carrying solution: water=1: 3
Nano-carrier (F3 (3))
Nano-carrier load Whitfield's ointment (F4 (3))
Nano-carrier load matrine (F5 (3))
Nano-carrier loaded with nano TiO
2(F6 (3))
The bright amine (F7 (3)) of nano-carrier load rice
Composite group of c (being that two kinds of solution of above b group mix):
G1：F7(2)+F4(2)
G2：F7(2)+F6(2)
G3：F5(2)+F4(2)
G4：F5(2)+F6(2)
Testing sequence:
(1) nano-carrier load agricultural chemicals infusion packet group:
A. the nano-carrier infusion packet is that 100 of F1 groups are carried out bagging, and the sack of 100 blank no any processing is checked in addition;
B. nano-carrier parcel Whitfield's ointment infusion packet is that 100 of F2 groups are carried out bagging, and the sack of 100 blank no any processing is checked in addition.
(2) nano-carrier load agricultural chemicals soaks the fruit group:
A. high density group: F3 (2), F4 (2), F5 (2), F6 (2), F7 (2) respectively every group soak 200 fruits, soak medicine every other month once, two-layer altogether, wherein 100 fruits carry out bagging and handle, and do not do bagging and handle for other 100;
B. low concentration group: F3 (3), F4 (3), F5 (3), F6 (3), F7 (3) respectively every group soak 200 fruits, soak medicine every other month once, two-layer altogether, wherein 100 fruits carry out bagging and handle, and do not do bagging and handle for other 100;
C. composite group: G1, G2, G3, G4 be 200 fruits of every group of immersion respectively, soak medicine every other month once, and be two-layer altogether, and wherein 100 fruits carry out the bagging processing, do not do the bagging processing for other 100;
Experimental result:
The first-class fruit of each group of this experiment of table 1 proportion
Extension rate: 1: 2
Extension rate: 1: 3
Composite:
Annotate: p＜0.01
Test-results shows:
Used nano-carrier load agricultural chemicals to soak fruit and comprised that bare and medicine carrying all have very significantly disease resistance effect; Disease resisting effect can reach 75% (ratio of the shared mango sum of high-quality mango that appearance is bright and new), and the setting ratio of the high-quality mango of control group (simple bagging) has only 21%.
Even only use blank nano-carrier to soak no matter bagging or not bagging behind the fruit, effect has all improved about 62%;
The disease-resistant fruit retention benefit of field experiment data presentation nanometer film and nano drug-carrying film has been practiced thrift the input of setting up expensive equipment such as greenhouse effectively, has practiced thrift manpower and materials, can bring considerable benefit for the orchard.
The foregoing description is a preferred implementation of the present invention; But embodiment of the present invention is not restricted to the described embodiments; Other any do not deviate from change, the modification done under spirit of the present invention and the principle, substitutes, combination, simplify; All should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. long-chain hydrophobic grouping grafting Preparation of Carboxymethylchitosan method is characterized in that may further comprise the steps:
(1) long-chain alcohol, sodium hydroxide and cetyl trimethylammonium bromide are mixed, stir heating and melting; Splash into epoxy chloropropane again; At 60-65 ℃ of reaction 2-4h, filter then, collect filtrating and use water washing; The drying of filtrating is dewatered, and the alkoxyl group glycidyl ether that solvent obtains long-chain is fallen in redistillation;
(2) Pottasium Hydroxide is dissolved in obtains solution A in the Virahol; CMS is joined in the solution A, add the alkoxyl group glycidyl ether of the long-chain that step (1) prepares again, 60-65 ℃ of reaction 12-24h down; Filter then; Collect filtrating and use absolute ethanol washing, drying obtains long-chain hydrophobic grouping grafting CMS;
The described long-chain alcohol of step (1) is a monohydroxy-alcohol, and its carbonatoms is 8-32;
The mol ratio of said long-chain alcohol of step (1) and sodium hydroxide is 1: 1-3;
The mol ratio of said long-chain alcohol of step (1) and cetyl trimethylammonium bromide is 1: 0.010-0.030;
The mol ratio of said long-chain alcohol of step (1) and epoxy chloropropane is 1: 1-3;
The mass ratio of the alkoxyl group glycidyl ether of said CMS of step (2) and long-chain is 1: 1-3.5.
2. long-chain hydrophobic grouping grafting Preparation of Carboxymethylchitosan method according to claim 1 is characterized in that:
The mol ratio of said long-chain alcohol of step (1) and sodium hydroxide is 1: 2;
The mol ratio of said long-chain alcohol of step (1) and cetyl trimethylammonium bromide is 1: 0.013.
3. long-chain hydrophobic group grafting Preparation of Carboxymethylchitosan method according to claim 1, it is characterized in that: the mol ratio of said long-chain alcohol of step (1) and epoxy chloropropane is 1: 2.
4. long-chain hydrophobic grouping grafting Preparation of Carboxymethylchitosan method according to claim 1 is characterized in that:
The temperature of the said water of step (1) is 50-70 ℃;
In the said solution A of step (2), the concentration of Pottasium Hydroxide is 0.1-0.2g/100mL.
5. long-chain hydrophobic group grafting Preparation of Carboxymethylchitosan method according to claim 1 is characterized in that:
The mass ratio of the alkoxyl group glycidyl ether of said CMS of step (2) and long-chain is 1: 2.1;
The temperature of the said absolute ethyl alcohol of step (2) is 50-70 ℃.
6. a long-chain hydrophobic grouping grafting CMS is prepared by each described long-chain hydrophobic grouping grafting Preparation of Carboxymethylchitosan method of claim 1-5.
7. the preparation method of a medicament-carried nano micelle solution, it is characterized in that may further comprise the steps: the weighting profit requires 6 described long-chain hydrophobic grouping grafting CMSs to be dissolved in the water, and obtains solution B; The thing of getting it filled is dissolved in water or the ethanol, obtains solution C; Solution C slowly is added dropwise in the solution B, steams then and remove ethanol, obtain medicament-carried nano micelle solution.
8. the preparation method of medicament-carried nano micelle solution according to claim 7 is characterized in that:
The concentration of long-chain hydrophobic grouping grafting CMS is 1-6g/L in the said solution B;
The concentration of said solution C Chinese traditional medicine is 10g/L;
The volume ratio of said solution C and solution B is 1: 10.
9. medicament-carried nano micelle solution is that the preparation method by claim 7 or 8 described medicament-carried nano micelle solution prepares.
10. the application of the described medicament-carried nano micelle solution of claim 9 is characterized in that: with the described medicament-carried nano micelle spray solution of claim 9 to plant surface.
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| CN102850469A (en) * | 2012-10-11 | 2013-01-02 | 山东大学 | O-carboxymethyl chitosan-stearic acid polymer as well as synthesis method thereof and application of polymer |
| CN104126576A (en) * | 2014-06-18 | 2014-11-05 | 南开大学 | Pesticide intercalated hydroxide salt nanohybrid and preparation method thereof |
| CN109438377A (en) * | 2018-12-13 | 2019-03-08 | 天津利安隆新材料股份有限公司 | A kind of method of Long carbon chain triazine-based ultraviolet absorbent of the low temperature process preparation containing ehter bond |
| CN109769803A (en) * | 2017-11-14 | 2019-05-21 | 北京纳威克生物科技开发有限公司 | A kind of pesticide polyelectrolyte nano-micelle and preparation method thereof |
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| CN102850469A (en) * | 2012-10-11 | 2013-01-02 | 山东大学 | O-carboxymethyl chitosan-stearic acid polymer as well as synthesis method thereof and application of polymer |
| CN102850469B (en) * | 2012-10-11 | 2015-01-28 | 山东大学 | O-carboxymethyl chitosan-stearic acid polymer as well as synthesis method thereof and application of polymer |
| CN104126576A (en) * | 2014-06-18 | 2014-11-05 | 南开大学 | Pesticide intercalated hydroxide salt nanohybrid and preparation method thereof |
| CN104126576B (en) * | 2014-06-18 | 2016-03-30 | 南开大学 | A kind of agricultural chemicals intercalation hydroxide salt nano hybrid and preparation method thereof |
| CN109769803A (en) * | 2017-11-14 | 2019-05-21 | 北京纳威克生物科技开发有限公司 | A kind of pesticide polyelectrolyte nano-micelle and preparation method thereof |
| CN109438377A (en) * | 2018-12-13 | 2019-03-08 | 天津利安隆新材料股份有限公司 | A kind of method of Long carbon chain triazine-based ultraviolet absorbent of the low temperature process preparation containing ehter bond |
| CN109438377B (en) * | 2018-12-13 | 2020-06-09 | 天津利安隆新材料股份有限公司 | Method for preparing long-carbon-chain triazine ultraviolet absorbent containing ether bond by low-temperature method |
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