CN102406923A - Three Chinese medicaments capable of improving activity of nattokinase - Google Patents
Three Chinese medicaments capable of improving activity of nattokinase Download PDFInfo
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- CN102406923A CN102406923A CN2010102921277A CN201010292127A CN102406923A CN 102406923 A CN102406923 A CN 102406923A CN 2010102921277 A CN2010102921277 A CN 2010102921277A CN 201010292127 A CN201010292127 A CN 201010292127A CN 102406923 A CN102406923 A CN 102406923A
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- nattokinase
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Abstract
The invention discloses three Chinese medicaments capable of improving activity of nattokinase. The medicaments are characterized in that: the three medicaments are liquorice, astragalus mongholicus and indigowoad leaf, which can improve the activity of nattokinase, and are integrated with Chinese medicament properties, so that by matching one or more of the three Chinese medicaments with the nattokinase, the efficacy of the nattokinase in preventing and treating thrombus can be remarkably improved, and the safety of the nattokinase can be increased.
Description
Technical field
The present invention relates to three kinds of Chinese medicines that can improve natto kinase activity, belong to biological field.
Background technology
Nattokinase (nattokinase) is a kind of single chain serine protease that is produced by natto bacillus subtilis (Bacillus subtilis/natto), and 1987 by seeing discovery such as foreign firm.Research shows that nattokinase has fibrinolytic, can also activate intravital plasminogen, can prevent and treat thrombosis.
Because nattokinase has the advantage of using in the market that thrombolytic drug did not have, the enzyme activity that improves the units of product of nattokinase so has great importance to the popularization and the use of nattokinase.On fermentation mode, adopt liquid fermentation; Characteristics be operate easier, technology is simple, has solved the technical barrier that the nattokinase industrialization is amplified, and has adopted the industrial separation purification technique of producing the high-purity high-activity nattokinase simultaneously; Find the Chinese medicine that can improve natto kinase activity on this basis; The property of medicine of Chinese medicine is incorporated wherein, both reduced cost, improved drug effect and safety through compatibility again.
Radix Glycyrrhizae is a kind of tonification Chinese herbal medicine.Medicinal part is a root and rhizome, long 25～100cm, diameter 0.6～3.5cm.The feeble QI flavor is sweet and special.Function cures mainly heat-clearing and toxic substances removing, expelling phlegm for arresting cough, gastral cavity abdomen etc.Be grown in arid, semiarid desert steppe, desert edge and loess hill area more; The Radix Astragali originates in ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang, is the root of leguminous herbaceous plant's Radix Astagali, Radix Astragali, has effects such as invigorating QI to consolidate the body surface resistance, diuretic detumescent, expelling pus and toxin by strengthening QI, granulation promoting.Modern study shows that the Radix Astragali contains various trace elements such as Saponin, sucrose, polysaccharide, several amino acids, folic acid and selenium, zinc, copper.The enhancing human body immunity function arranged, protect the liver, diuresis, defying age, anti-stress, blood pressure lowering and antibacterial action more widely.Folium Isatidis is mainly used in pyretic toxicity and sends out diseases such as speckle, erysipelas, laryngopharynx swelling and pain, aphtha of the mouth and tongue, sore and toxic.This medicine extensive use is clinically in recent years controlled above-mentioned all diseases except that available, can be used for expectorant heat stagnation lung again, cough up phlegm yellow thick; Especially be usually used in popular second property encephalitis, but both single was applied to prevention, can cooperate Radix Bupleuri, Flos Lonicerae, Fructus Forsythiae, Radix Isatidis, Radix Scrophulariae, the Radix Rehmanniae etc. again.
Summary of the invention
The object of the present invention is to provide three kinds of Chinese medicines that can improve natto kinase activity.These three kinds of Chinese medicines can not only improve the activity of nattokinase; Also the property of medicine with Chinese medicine incorporates wherein; One or more and nattokinase through in these three kinds of Chinese medicines are compatible, can significantly improve the effect of nattokinase prevention and treatment thrombosis, and can improve its safety.
Technical scheme of the present invention is:
1, employed nattokinase is that superior strain is produced through liquid fermentation in the soybean cake enzymolysis solution, and enzyme is lived and is 1630IU/mL.
2, employed nattokinase through separation and purification after, the enzyme of pure article is lived and is 30000IU/mg.
3, the Radix Glycyrrhizae extract and the nattokinase of variable concentrations is compatible, the enzyme work of nattokinase can improve 5-10%.
4, the Radix Astragali extractive solution and the nattokinase of variable concentrations is compatible, the enzyme work of nattokinase can improve 10-12%.
5, the Folium Isatidis extracting solution and the nattokinase of variable concentrations is compatible, the enzyme work of nattokinase can improve 10-15%.
6, Radix Glycyrrhizae, the Radix Astragali, the combination in twos by a certain percentage of Folium Isatidis extracting solution of variable concentrations is compatible with nattokinase, the enzyme work of nattokinase can improve 15-25%.
7, Radix Glycyrrhizae, the Radix Astragali, the Folium Isatidis extracting solution with variable concentrations makes up by a certain percentage, and be compatible with nattokinase again, and the enzyme work of nattokinase can improve 30-40%.
Benefit of the present invention:
1, significantly improves the activity of nattokinase, can improve the bioavailability of preparation, reduce medication dose;
2, improve the safety of nattokinase, controlled thrombolytic speed to prevent the generation of ischemical reperfusion injury;
3, improved the stability of nattokinase.
The present invention shows through zoopery: have tangible thrombolytic effect, the effect behind the compatibility obviously is superior to the nattokinase of same dose.
The specific embodiment
1, the mixed liquor that contains 1mg/mL Radix Glycyrrhizae and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 6.2%.
2, the mixed liquor that contains the 1mg/mL Radix Astragali and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 10.8%.
3, the mixed liquor that contains 1mg/mL Folium Isatidis and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 11.4%.
4, the mixed liquor that contains 1mg/mL Radix Glycyrrhizae, the 1mg/mL Radix Astragali and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 16.9%.
5, the mixed liquor that contains 1mg/mL Radix Glycyrrhizae, 1mg/mL Folium Isatidis and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 17.4%.
6, the mixed liquor that contains the 1mg/mL Radix Astragali, 1mg/mL Folium Isatidis and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 23.2%.
7, the mixed liquor that contains 1mg/mL Radix Glycyrrhizae, the 1mg/mL Radix Astragali and 1mg/mL Folium Isatidis and 3000IU/mL nattokinase, the enzyme work of nattokinase has improved 33.5%.
Claims (8)
1. three kinds of Chinese medicines that can improve natto kinase activity is characterized in that these three kinds of Chinese medicines are Radix Glycyrrhizae, the Radix Astragali, Folium Isatidis.
2. require the described Chinese medicine that can improve natto kinase activity according to right 1, it is characterized in that the nattokinase of selecting is that superior strain is produced through liquid fermentation in the soybean cake enzymolysis solution, enzyme work reaches 1630IU/mL.
3. require the described Chinese medicine that can improve natto kinase activity according to right 1,2, the nattokinase that it is characterized in that selecting through separation and purification after, the enzyme work of pure article reaches 30000IU/mg.
4. require the described Chinese medicine that can improve natto kinase activity according to right 1,2,3, it is characterized in that Radix Glycyrrhizae extract and nattokinase are compatible, the enzyme work of nattokinase can improve 5-10%.
5. require the described Chinese medicine that can improve natto kinase activity according to right 1,2,3,4, it is characterized in that Radix Astragali extractive solution and nattokinase are compatible, the enzyme work of nattokinase can improve 10-12%.
6. require the described Chinese medicine that can improve natto kinase activity according to right 1,2,3,4,5, it is characterized in that Folium Isatidis extracting solution and nattokinase are compatible, the enzyme work of nattokinase can improve 10-15%.
7. require the described Chinese medicine that can improve natto kinase activity according to right 1,2,3,4,5,6; It is characterized in that the combination in twos by a certain percentage of Radix Glycyrrhizae, the Radix Astragali, Folium Isatidis extracting solution is compatible with nattokinase, the enzyme work of nattokinase can improve 15-25%.
8. require the described Chinese medicine that can improve natto kinase activity according to right 1,2,3,4,5,6,7; It is characterized in that Radix Glycyrrhizae, the Radix Astragali, Folium Isatidis extracting solution make up by a certain percentage; Compatible with nattokinase again, the enzyme work of nattokinase can improve 30-40%.
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| CN2010102921277A CN102406923A (en) | 2010-09-26 | 2010-09-26 | Three Chinese medicaments capable of improving activity of nattokinase |
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| CN2010102921277A CN102406923A (en) | 2010-09-26 | 2010-09-26 | Three Chinese medicaments capable of improving activity of nattokinase |
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| CN102406923A true CN102406923A (en) | 2012-04-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039295A (en) * | 2016-06-24 | 2016-10-26 | 方捷 | Nattokinase composition for preventing thrombosis and thrombolysis and preparation method thereof |
| CN109718219A (en) * | 2019-03-06 | 2019-05-07 | 武汉轻工大学 | A kind of Nattokinase enteric coatel tablets and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872232A (en) * | 2005-06-01 | 2006-12-06 | 天津天士力制药股份有限公司 | Composition of medication for curing cerebrovascular disease, and preparation method |
| WO2007090393A2 (en) * | 2006-02-10 | 2007-08-16 | Biogenerics Pharma Gmbh | Microtablet-based pharmaceutical preparation |
| CN101810336A (en) * | 2010-04-30 | 2010-08-25 | 广东仙乐制药有限公司 | Chewable soft capsules and method for preparing same |
-
2010
- 2010-09-26 CN CN2010102921277A patent/CN102406923A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872232A (en) * | 2005-06-01 | 2006-12-06 | 天津天士力制药股份有限公司 | Composition of medication for curing cerebrovascular disease, and preparation method |
| WO2007090393A2 (en) * | 2006-02-10 | 2007-08-16 | Biogenerics Pharma Gmbh | Microtablet-based pharmaceutical preparation |
| CN101810336A (en) * | 2010-04-30 | 2010-08-25 | 广东仙乐制药有限公司 | Chewable soft capsules and method for preparing same |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106039295A (en) * | 2016-06-24 | 2016-10-26 | 方捷 | Nattokinase composition for preventing thrombosis and thrombolysis and preparation method thereof |
| CN109718219A (en) * | 2019-03-06 | 2019-05-07 | 武汉轻工大学 | A kind of Nattokinase enteric coatel tablets and preparation method thereof |
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Application publication date: 20120411 |