CN102406100A - Method for degradation of deoxynivalenol - Google Patents
Method for degradation of deoxynivalenol Download PDFInfo
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- CN102406100A CN102406100A CN2011103850305A CN201110385030A CN102406100A CN 102406100 A CN102406100 A CN 102406100A CN 2011103850305 A CN2011103850305 A CN 2011103850305A CN 201110385030 A CN201110385030 A CN 201110385030A CN 102406100 A CN102406100 A CN 102406100A
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Abstract
The invention relates to a method for degradation of deoxynivalenol (DON), wherein bacterial powder of bacillus licheniformis is adopted to degrade the DON in mildewy grains. With the method of the present invention, the DON in the mildewy grains can be effectively degraded, and the application values of the mildewy grains are restored. According to the present invention, with detecting the effect of the DON degrading by the bacterial powder of the bacillus licheniformis under an in vitro simulation environment of the animal gastrointestinal tract, the optimal degradation conditions for the DON by the bacterial powder of the bacillus licheniformis are explored, and the method provides a solid foundation for further development of the bacterial powder of the bacillus licheniformis into the biological antidote, wherein the biological antidote is provided for the degradation of zeranol in grains and corns.
Description
Technical field
The invention belongs to the biologic detoxication technical field, be specifically related to a kind of method of the deoxynivalenol of degrading, promptly utilize the bacillus licheniformis bacterium powder deoxynivalenol of degrading.
Background technology
Deoxynivalenol (deoxynivalenol; DON); Belonging to the trichothecene class, is to plant a kind of chemical constitution toxic metabolite product similar with biologically active that sickle-like bacteria produces by some, and it mainly produces bacterium is Fusarium graminearum (Fusarium graminearum).DON exists and cereal crops such as the wheat that polluted by sickle-like bacteria, barley, corn, also is present in the cereal product, and humans and animals can produce poisonous effect widely after eating by mistake by the Cereals class of this endotoxin contamination.In addition, it is often gone back and other mycotoxin such as aflatoxin pollute crops jointly, can influence each other behind the entering human body.There are some researches show that in recent years DON maybe be relevant with human esophagus cancer, IgA ephrosis, the health of the mankind and animal is constituted serious threat.DON also maybe be influential to immune system, and tangible embryotoxicity and certain teratogenesis are arranged.The serious harm property of DON has caused the generally attention of various countries, but because DON belongs to the field toxin, is accompanied by the growth course of cereal and produces, and therefore is difficult for prevention, can only remove through antidote.
Removal method for DON mainly comprises chemical method, physical method and microbial process at present.But it is big that chemical method exists the loss of cereal nutriment, and shortcomings such as cost height are seldom used in the production.Physical method mainly is to reach the effect that reduces toxicity through the inorganic mineral physical absorption; But mineral matter also can adsorb the nutritional labeling in the cereal when removing toxin; Together be discharged in the environment, cause nutriment to run off, have a strong impact on the safety of ecological environment.And microbial process is to utilize the secondary metabolites of microorganisms or the secreted toxophore that born of the same parents are interior, ectoenzyme decomposes destruction DON; Reduce its toxicity; Condition is gentle relatively, can not influence the quality and the nutrition of cereal, has represented the new direction of DON detoxifcation research.
In-vitro simulated animal gastrointestinal tract environment method is adopted in research to the detoxifcation of DON microorganism usually, promptly at 37 ℃, is 2.0～2.5 cultivations, 1～2h at gastric acid environment pH, and leading portion enteron aisle acid or alkali environment pH processes the microbe culture system behind 6.5～7.0 cultivations, 3～4h.Finish DON content in the mensuration supernatant of back through in the microbe culture system, adding a certain amount of DON standard items, cultivating, confirm whether microorganism can transform or metabolism DON.Also have and report the adsorbance that can effectively reduce toxin to the washing times of microbial cells through increasing; And after with an organic solvent handling the cultured microorganism thalline; The toxin that almost can both combine with microorganism discharges, and these methods all are that the effect of scientific verification microbial degradation toxin provides certain foundation (Carolyn A h, HahiS E; Pasi E K; Et a1.Surface binding of aflatoxinB1 by lactic acid bacteria.Applied and Environmental Microbiology, 2001,67 (7): 3086-3091).
At present seldom about the report of microbial strains degraded DON; Found that excellent bacillus of anaerobism and agrobacterium can both partly transform DON; Can both reduce toxicity (the Fuchs E of DON to a certain extent; Binder E M; Heidler D.Krska R.Structural characterization of metabolites after themicrobial degradation of type Atrichothecenes by the bacterial strainBBSH 797.Food Additives and Contaminants, 2002,19 (4): 379-386.; ShimaJ, Takase S, Takahashi Y; Iwai Y; Fujimoto H, Yamazaki M, Ochi K.Noveldetoxification of the trichothecene mycotoxin deoxynivalenol by a soilbacterium isolated by enrichment culture.Applied and EnvironmentalMicrobiology; 1997,63 (10): 3825-3830).Therefore, the new microbial resources of the development and use biologic detoxication that carries out DON has important prospect and meaning.
Summary of the invention
The method that the purpose of this invention is to provide a kind of degraded deoxynivalenol (hereinafter to be referred as DON) is promptly utilized the bacillus licheniformis deoxynivalenol of degrading, and is set up degradation condition.
Utilize the bacillus licheniformis bacterium powder deoxynivalenol of degrading.
Described bacillus licheniformis can be selected for use available from Chinese common micro-organisms culture presevation administrative center, classification called after bacillus licheniformis (Bacillus licheniformis), and preserving number is: CGMCC1.807.
Method of the present invention is that the deoxynivalenol in the musty grain is degraded.
The method of degraded deoxynivalenol of the present invention, the mass percent of the addition of bacillus licheniformis bacterium powder is 0.2%, 1.5h degrades under 37 ℃, pH2.0 condition; Or bacillus licheniformis bacterium powder addition mass percent is 0.2%, and 3.0h degrades under 37 ℃, pH6.5 condition.
One aspect of the invention relates to a kind of bacillus licheniformis bacterium powder, and described bacillus licheniformis bacterium powder is bacillus licheniformis and diatomaceous mixed powder.
Described bacillus licheniformis bacterium powder is bacillus licheniformis and diatomaceous mixed powder, and wherein the bacillus licheniformis proportion is 60%-80%.
The present invention uses bacillus licheniformis bacterium powder deoxynivalenol is degraded, the deoxynivalenol in the musty grain of can degrading effectively, thereby the using value of recovery musty grain.The present invention simultaneously is through measuring the degradation effect of bacillus licheniformis bacterium powder to deoxynivalenol under in-vitro simulated gastrointestinal tract environment; Find out the best degradation condition of bacillus licheniformis bacterium powder to deoxynivalenol; For further it being developed as the biologic detoxication agent, the degraded that is used for cereal and feed deoxynivalenol provides solid foundation.
The specific embodiment
The applied reagent of the present invention can be used arbitrary money commercially available prod, for example deoxynivalenol (DON) standard items: purchase in sigma company, DON high-sensitivity detection kit DeoxynivalenolVeratox HS: available from Britain NEOGEN company; The compound method of used buffer solution is following:
1, the PBS:KCl:0.2g KH2PO4:0.204g Na2HPO42H2O:1.442gNaCl:8.066g of pH6.5 is dissolved in the 1000ml distilled water, and regulating the pH value is 6.5;
2, the PBS:KCl:0.2g KH2PO4:0.204g Na2HPO42H2O:1.442gNaCl:8.066g of pH2.0 is dissolved in the 1000ml distilled water, and regulating the pH value is 2.0;
3, pepsin solution liquid: accurately take by weighing pepsin (1: 10000) 1.333g, be dissolved in the PBS solution of 10mlpH2.0, fully stirring is dissolved it fully;
4, trypsin solution liquid: accurately take by weighing trypsase (1: 250) 1.0g, be dissolved in the PBS solution of 10mlpH6.5, fully stirring is dissolved it fully.
Below in conjunction with specific embodiment method of the present invention is described in detail.
The preparation of embodiment 1 bacillus licheniformis bacterium powder
1, the inclined-plane is cultivated
In the test tube that broth agar culture medium (beef extract 0.3%, peptone 1%, sodium chloride 0.5%, agar 1.5%) is housed, insert 1.807,37 ℃ of bacillus licheniformis bacterial classification CGMCC, cultivated 24 hours.
2, the preparation of seed liquor
Bacillus licheniformis on the slant medium is transferred to shakes in the bottle, adopt broth bouillon (beef extract 0.3%, peptone 1%, sodium chloride 0.5%), 37 ℃, pH7.0,200r/min cultivated 24 hours.
3, fermentation procedure:
The 1T seeding tank:
Fermentation medium: broth bouillon
Condition of culture: inoculum concentration 8%, 60%, 37 ℃ of liquid amount, 180rpm cultivates 8-10h.
The 15T fermentation tank:
Fermentation medium: dregs of beans 4%, corn flour 2%, manganese sulfate 0.05%, magnesium sulfate 0.05%.
Condition of culture: 5%, 37 ℃ of inoculum concentration, 150rpm cultivates 24-26h.
Stop training: when the gemma comparative maturity, the gemma rate reaches more than 90%.
4, postprocessing working procedures
Zymotic fluid is warming up to 50 ℃, add solid calcium chloride and sodium hydrogen phosphate each 0.3%, change storage tank over to behind the stirring 30min, add 3% diatomite, plate compression.Filter cake is dry in boiling drier, 90 ℃ of intake air temperatures, 50 ℃ of air outlet temperature, dry 15s, 80 orders are pulverized.
Embodiment 2 bacillus licheniformis bacterium powder are to the degradation of DON standard items
1. the addition of bacillus licheniformis bacterium powder is to the influence of DON standard items degradation rate
According to chyme time of staying in animal intestines and stomach, be determined under simulated animal stomach, the intestinal environment bacillus licheniformis bacterium powder addition respectively the degradation rate of DON standard items is confirmed the optimum addition of bacterium powder.
1.1 bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate in SGF
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, concentration is the DON standard items 0.13ml of 100ppm, adds 0.05% respectively; 0.10%, 0.15,0.2%, 0.3%; 0.4% (mass ratio) bacillus licheniformis bacterium powder, every group of two repetitions, 37 ℃, 4h is cultivated in concussion; After cultivating end, 4500r/min is centrifugal, and supernatant filters the back with funnel and collects, as the liquid to be measured of DON in the supernatant; The distilled water that adds 25ml in the deposition, concuss 5min filters and collects filtrating, as deposition desorption DON liquid to be measured.Preserve in 4 ℃ of refrigerators before measuring.Utilize DON content in DON high-sensitivity detection kit measurement supernatant and the deposition, and calculate the degradation rate of bacillus licheniformis to DON, experimental result is seen table 1.
1.2 bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, concentration is the DON standard items 0.13ml of 100ppm, adds 0.05% respectively; 0.10%, 0.15%, 0.2%, 0.3%; 0.4% (mass ratio) bacillus licheniformis bacterium powder, every group of two repetitions, 37 ℃; 8h is cultivated in concussion, cultivates described in the processing such as step 1.1 after finishing, and experimental result is seen table 2.
Table 1 under in-vitro simulated gastric environment bacillus licheniformis bacterium powder addition to the influence of DON standard items degradation rate
| Bacterium powder addition | 0.05 | 0.1 | 0.15 | 0.2 | 0.3 | 0.4 |
| The DON degradation rate | 10.2 | 13.9 | 20.3 | 25.7 | 24.9 | 25.1 |
Table 2 under in-vitro simulated intestines environment bacillus licheniformis bacterium powder addition to the influence of DON standard items degradation rate
| Bacterium powder addition % | 0.05 | 0.1 | 0.15 | 0.2 | 0.3 | 0.4 |
| DON degradation rate % | 11.7 | 18.5 | 34.1 | 41.9 | 42.2 | 42.8 |
By table 1, table 2 can find out that when lichens bacillus bacterium powder addition was lower than 0.2%, along with the increase of addition, the DON degradation rate improved constantly; When addition was higher than 0.2%, along with the increase of addition, the DON degradation rate tended towards stability, so the righttest addition of bacillus licheniformis bacterium powder is 0.2%.
2. the degradation time of bacillus licheniformis bacterium powder is to the influence of DON standard items degradation rate
Under simulated animal stomach, intestinal environment, the bacillus licheniformis bacterium powder of measuring the righttest addition respectively when different degradation time to the degradation rate of DON, to confirm the best degradation time of bacillus licheniformis bacterium powder.
2.1 bacillus licheniformis bacterium powder confirming in SGF to the DON degradation time
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, concentration is the DON standard items 0.13ml of 100ppm, adds the bacillus licheniformis bacterium powder of 0.2% (mass ratio); 37 ℃, concussion is cultivated, respectively at 15min, and 30min; 45min, 60min, 90min measures the DON content in supernatant and the deposition during 120min; The degradation rate of bacillus licheniformis bacterium powder to DON calculated in every group of two repetitions respectively, and experimental result is seen table 3.
2.2 bacillus licheniformis bacterium powder confirming in simulated intestinal fluid to the DON degradation time
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, concentration is the DON standard items 0.13ml of 100ppm, adds the bacillus licheniformis bacterium powder of 0.2% (mass ratio); 37 ℃, concussion is cultivated, respectively at 30min, and 60min; 90min, 120min, 180min measures the DON content in supernatant and the deposition during 240min; Every group of two repetitions, other steps are with 2.1, and experimental result is seen table 4.
Bacillus licheniformis bacterium powder degradation time is to the influence of DON standard items degradation rate under the in-vitro simulated gastric environment of table 3
| Degradation time/min | 15 | 30 | 45 | 60 | 90 | 120 |
| DON degradation rate % | 12.2 | 13.1 | 18.7 | 22.9 | 25.1 | 24.9 |
Bacillus licheniformis bacterium powder degradation time is to the influence of DON standard items degradation rate under the in-vitro simulated intestines environment of table 4
| Degradation time/min | 30 | 60 | 90 | 120 | 180 | 240 |
| DON degradation rate % | 14.9 | 17.5 | 33.1 | 40.9 | 43.2 | 42.8 |
By table 3, table 4 can find out that bacterium powder addition is at 0.2% o'clock, and along with the prolongation of degradation time, the degradation rate of DON improves constantly; In in-vitro simulated gastric environment, when degradation time >=90min, the degradation rate of DON tends towards stability; In in-vitro simulated intestines environment, during degradation time >=3h, the DON degradation rate tends towards stability.
In sum, the optimum condition of bacillus licheniformis bacterium powder degraded DON standard items is: 0.2%, 37 ℃ of bacterium powder addition, pH2.0, degraded 1.5h; 37 ℃, pH 6.5, degraded 3.0h, and this moment is the highest to the total degradation rate of DON standard items, reaches 68.3%.Wherein the computing formula of degradation rate is following:
The total degradation rate: bacillus licheniformis bacterium powder is to the degradation rate sum of DON under in-vitro simulated stomach, the intestinal environment
Implement the degradation of real 3 bacillus licheniformis bacterium powder to DON in the mouldy corn
1. the total quantitative determination of DON in the mouldy corn
Mouldy corn is pulverized the back and is crossed 20 mesh sieves, and the assay method mensuration DON content that requires according to the Deoxynivalenol VeratoxHS of Britain NEOGEN company (DON high-sensitivity detection kit) is: 2281.78ppb
2. bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate in the mouldy corn
According to chyme time of staying in animal intestines and stomach, be determined at respectively under simulated animal stomach, the intestinal environment, bacillus licheniformis bacterium powder addition is confirmed the optimum addition of bacterium powder to the influence of the degradation rate of DON in the mouldy corn.
2.1 bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate in SGF
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, mouldy corn 10g adds 0.05%, 0.10% respectively; 0.15%, 0.2%, 0.3%, 0.4% (mass ratio) bacillus licheniformis bacterium powder is set up control group simultaneously; Only add mouldy corn, every group of two repetitions, 37 ℃, 4h is cultivated in concussion; After cultivating end, centrifugal at 4500r/min, supernatant filters the back with funnel and collects, as the liquid to be measured of DON in the supernatant; The distilled water that adds 25ml in the deposition, concuss 5min filters and collects filtrating, as deposition desorption DON liquid to be measured.Preserve in 4 ℃ of refrigerators before measuring.Utilize DON content in DON high-sensitivity detection kit measurement supernatant and the deposition, and calculate the degradation rate of bacillus licheniformis bacterium powder to DON, experimental result is seen table 5.
2.2 bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate in simulated intestinal fluid
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, mouldy corn 10g adds 0.05% respectively; 0.10%, 0.15%, 0.2%, 0.3%; 0.4% (mass ratio) bacillus licheniformis bacterium powder is set up control group simultaneously, only adds mouldy corn, every group of two repetitions; At 37 ℃, 8h is cultivated in concussion, cultivates described in the processing such as step 2.1 after finishing, and experimental result is seen table 6.
Table 5 under in-vitro simulated gastric environment bacillus licheniformis bacterium powder addition to the influence of DON degradation rate
Bacillus licheniformis bacterium powder addition is to the influence of DON degradation rate under the in-vitro simulated intestines environment of table 6
| Bacterium powder addition % | 0.05 | 0.10 | 0.15 | 0.20 | 0.30 | 0.40 |
| DON degradation rate % | 10.7 | 17.5 | 24.1 | 45.9 | 46.2 | 46.3 |
By table 5, table 6 can find out that when lichens bacillus bacterium powder addition was lower than 0.2%, along with the increase of addition, the degradation rate of DON improved constantly in the mouldy corn; When addition was higher than 0.2%, the degradation rate of DON tended towards stability, so the righttest addition of bacillus licheniformis bacterium powder is 0.2%.3. bacillus licheniformis bacterium powder degradation time is to the influence of DON degradation rate in the mouldy corn
The bacillus licheniformis bacterium powder of under in-vitro simulated stomach, intestinal environment, measuring the righttest addition respectively under different degradation times to the degradation rate of DON, to confirm the best degradation time of bacillus licheniformis bacterium powder.
3.1 bacillus licheniformis bacterium powder confirming in SGF to DON degradation time in the mouldy corn
In triangular flask, add the pH2.0PBS buffer solution 24ml after sterilizing, add pepsin solution 0.25ml, mouldy corn 10g, the bacillus licheniformis bacterium powder of adding 0.2%, 37 ℃; Concussion is cultivated, respectively at 15min, and 30min, 45min; 60min, 90min measures the DON content in supernatant and the deposition during 120min, set up control group simultaneously; Only add mouldy corn, the degradation rate of bacillus licheniformis bacterium powder to DON calculated in every group of two repetitions respectively, and experimental result is seen table 7.
3.2 bacillus licheniformis bacterium powder confirming in simulated intestinal fluid to DON degradation time in the mouldy corn
Add the pH6.5PBS buffer solution 24ml after sterilizing in the triangular flask, add trypsin solution 0.25ml, mouldy corn 10g, the bacillus licheniformis bacterium powder of adding 0.2%; 37 ℃, concussion is cultivated, respectively at 30min, and 60min; 90min, 120min, 180min measures the DON content in supernatant and the deposition during 240min; Every group of two repetitions, other steps are with 3.1, and experimental result is seen table 8.
Bacillus licheniformis bacterium powder degradation time is to the influence of DON degradation rate under the in-vitro simulated gastric environment of table 7
| Degradation time/min | 15 | 30 | 45 | 60 | 90 | 120 |
| DON degradation rate % | 10.2 | 12.7 | 16.9 | 23.9 | 25.1 | 24.9 |
Table 8 under in-vitro simulated intestines environment bacillus licheniformis bacterium powder degradation time to the influence of DON degradation rate
| Degradation time/min | 30 | 60 | 90 | 120 | 180 | 240 |
| DON degradation rate % | 13.9 | 16.9 | 24.1 | 35.9 | 41.2 | 41.9 |
By table 7, table 8 can find out that bacterium powder addition is at 0.2% o'clock, and along with the prolongation of degradation time, the degradation rate of DON improves constantly in the mouldy corn, and in in-vitro simulated gastric environment, when degradation time >=90min, the degradation rate of DON tends towards stability; In in-vitro simulated intestines environment, during degradation time >=3h, the degradation rate of DON tends towards stability.
In sum, bacillus licheniformis bacterium powder is degraded, and the optimum condition of DON is in the mouldy corn: 0.2%, 37 ℃ of bacterium powder addition, pH2.0, degraded 1.5h; 37 ℃, pH6.5, degraded 3.0h, this moment is the highest to the total degradation rate of DON in the mouldy corn, reaches 66.3%.
Embodiment 4, diatomite promote the degradation of bacillus licheniformis to DON
Get zymotic fluid described in embodiment 1 step 4, through centrifugal, washing, freeze drying makes the pure bacterium powder of bacillus licheniformis 100g;
Under simulated animal stomach, intestinal environment, measure the influence of diatomite to bacillus licheniformis degraded DON.4.1 diatomite is to the influence of bacillus licheniformis degraded DON in SGF
In triangular flask, add the pH2.0PBS buffer solution 240ml after sterilizing, add pepsin solution 2.5ml, concentration is the pure bacterium powder of bacillus licheniformis of the DON standard items 1.3ml and 0.2% (mass ratio) of 100ppm; Add 0% respectively then; 0.05%, 0.1%, 0.2%; 0.5% (mass ratio) diatomite, every group of two repetitions; Not add pure bacterium powder, only add 0%, 0.05%, 0.1%, 0.2%, 0.5% (mass ratio) diatomaceous experimental group and be contrast, 37 ℃, 1.5h is cultivated in concussion, and after cultivation finished, 4500r/min was centrifugal, and supernatant filters the back with funnel and collects, as the liquid to be measured of DON in the supernatant; The distilled water that adds 25ml in the deposition, concuss 5min filters and collects filtrating, as deposition desorption DON liquid to be measured.Preserve in 4 ℃ of refrigerators before measuring.Utilize DON content in DON high-sensitivity detection kit measurement supernatant and the deposition, and calculate the degradation rate of DON, experimental result is seen table 9.
4.2 diatomite is to the influence of bacillus licheniformis degraded DON in simulated intestinal fluid
Add the pH6.5PBS buffer solution 240ml after sterilizing in the triangular flask, add trypsin solution 2.5ml, concentration is the pure bacterium powder of bacillus licheniformis of the DON standard items 1.3ml and 0.2% (mass ratio) of 100ppm; Add 0% respectively then; 0.05%, 0.1%, 0.2%; 0.5% (mass ratio) diatomite, every group of two repetitions; Not add pure bacterium powder, only add 0%, 0.05%, 0.1%, 0.2%, 0.5% (mass ratio) diatomaceous experimental group and be contrast, 37 ℃, 3h is cultivated in concussion, cultivates described in the processing such as step 4.1 after finishing, and experimental result is seen table 10.
Table 9 under in-vitro simulated gastric environment diatomaceous addition to the influence of bacillus licheniformis degraded DON
"-" expression DON degradation rate is less than 0.001%
Diatomaceous addition is to the influence of bacillus licheniformis degraded DON under the in-vitro simulated intestines environment of table 10
"-" expression DON degradation rate is less than 0.001%
By table 9, table 10 can find out that diatomite can promote the degradation of bacillus licheniformis to DON, and the pure bacterium powder of bacillus licheniformis and diatomaceous mass ratio are that 2: 1 o'clock degradation effects are best.
Use for ease, can the pure bacterium powder of bacillus licheniformis directly be mixed with diatomite, the bacillus licheniformis proportion is 60%-80% in the Mixed Microbes powder of preparation.
Claims (7)
1. the method for the deoxynivalenol of degrading is characterized in that, is with the bacillus licheniformis bacterium powder deoxynivalenol of degrading.
2. the method for claim 1 is characterized in that described bacillus licheniformis bacterium powder is that the deoxynivalenol in the musty grain is degraded.
3. method as claimed in claim 2 is characterized in that the addition mass percent of described bacillus licheniformis bacterium powder in musty grain is 0.2%, and 1.5h degrades under 37 ℃, pH2.0 condition.
4. method as claimed in claim 2 is characterized in that the addition mass percent of described bacillus licheniformis bacterium powder in musty grain is 0.2%, and 3.0h degrades under 37 ℃, pH6.5 condition.
5. like each described method among the claim 2-4, it is characterized in that described musty grain is mouldy corn.
6. the bacillus licheniformis bacterium powder of using in the said method of claim 1 is bacillus licheniformis and diatomaceous mixed powder.
7. bacillus licheniformis bacterium powder as claimed in claim 6 is characterized in that described bacillus licheniformis proportion is 60%-80%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102816745A (en) * | 2012-09-11 | 2012-12-12 | 国家粮食局科学研究院 | Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof |
| CN108251386A (en) * | 2016-12-29 | 2018-07-06 | 中粮营养健康研究院有限公司 | A kind of method of vomitoxin degrading enzyme and its gene and preparation method and application and vomitoxin of degrading |
| CN109136143A (en) * | 2018-09-11 | 2019-01-04 | 河南工业大学 | One plant of vomitoxin degradation bacteria and its application |
| CN113755410A (en) * | 2021-11-10 | 2021-12-07 | 中国科学院天津工业生物技术研究所 | Bacillus licheniformis for degrading vomitoxin and application thereof |
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2011
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| 杨史良: "益生菌清除脱氧雪腐镰刀菌烯醇的作用研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816745A (en) * | 2012-09-11 | 2012-12-12 | 国家粮食局科学研究院 | Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof |
| CN102816745B (en) * | 2012-09-11 | 2014-02-05 | 国家粮食局科学研究院 | Deoxynivalenol toxin degrading enzyme as well as encoding gene and application thereof |
| CN108251386A (en) * | 2016-12-29 | 2018-07-06 | 中粮营养健康研究院有限公司 | A kind of method of vomitoxin degrading enzyme and its gene and preparation method and application and vomitoxin of degrading |
| CN108251386B (en) * | 2016-12-29 | 2021-09-24 | 中粮营养健康研究院有限公司 | Vomitoxin degrading enzyme, gene thereof, preparation method and application thereof, and method for degrading vomitoxin |
| CN109136143A (en) * | 2018-09-11 | 2019-01-04 | 河南工业大学 | One plant of vomitoxin degradation bacteria and its application |
| CN113755410A (en) * | 2021-11-10 | 2021-12-07 | 中国科学院天津工业生物技术研究所 | Bacillus licheniformis for degrading vomitoxin and application thereof |
| CN113755410B (en) * | 2021-11-10 | 2022-03-29 | 中国科学院天津工业生物技术研究所 | Bacillus licheniformis for degrading vomitoxin and application thereof |
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