CN102406091A - Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof - Google Patents
Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof Download PDFInfo
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- CN102406091A CN102406091A CN2011103949749A CN201110394974A CN102406091A CN 102406091 A CN102406091 A CN 102406091A CN 2011103949749 A CN2011103949749 A CN 2011103949749A CN 201110394974 A CN201110394974 A CN 201110394974A CN 102406091 A CN102406091 A CN 102406091A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a composite preparation for improving fish body transportation stress capability as well as a preparation method and application thereof. The composite preparation for improving fish body transportation stress capability comprises the following ingredients in parts by weight: 40-60 parts of tryptophan, 4-8 parts of nucleotide, 20-30 parts of Schisandra chinensis, 0.5-1.5 parts of tea polyphenol, 1-3 parts of vitamin E and 14.5-17.5 parts of carrier. The preparation method comprises the following steps: preparing the ingredients in parts by weight; fully and evenly mixing all the ingredients; and smashing to prepare the composite preparation of dry powder of 100-120 meshes. The composite preparation is used in a culture water body before the fish body is transported and in a transportation water body in the transportation process, and the use concentration is 10-20ppm. The preparation method has the advantages of low energy consumption, simple technology, low production cost and the like, and the composite preparation has the functions of improving fish body transportation stress resistance capability and increasing the survival rate.
Description
Technical field
The present invention relates to a kind of compound formulation that improves fish body transport stress ability, belong to technical field of aquaculture.
Background technology
In aquaculture, the transportation of the fish continual influence that receives some extraneous factors, particularly environment temperature, cultivation density, breeding water body chemokines, fish body, supporting etc. temporarily stress.As other vertebrate; The transport stress effect of fish body has great influence to the physiological dynamic equilibrium of fish; Serious stress make fish body lymphocyte and quantity of leucocyte reduce, and influence its breeding, growth, disease-resistant and even survival ability, cause the morbidity of fish body; Even dead, bring the big economic loss of tool to aquaculture.
A name at me is called " a kind of preparation method of anti-stress agent for fishing "; Application number stress preparation for disclosing a kind of use that glutamine dipeptide, Chinese rhubarb extract, vitamin C, vitamin E etc. process anti-in the patent document of " 200810195618.2 ", and it can improve the anti-stress ability of fish body.Be called " a kind of alimentation composition that improves anti-stress ability of aquacultural animals and preparation method thereof, application " in name; Application number discloses a kind of compound formulation that uses vitamin C, lactose, lubricant etc. to process in the patent document of " 200810153954.0 ", and it can improve the anti-stress ability of fish body.Be called " reduce Chinese medicine composition of stress response of aquatic animals and preparation method thereof, use " in name; Application number discloses a kind of middle medicine composite preparation that uses rheum officinale, spina date seed, immune enhancer etc. to process in the patent document of " 200910228096.6 ", and it can improve the anti-stress ability of fish body.Be called in name in " a kind of used for aquiculture water body splash type Antistress agent "; Application number is for disclosing a kind of water body that uses glucose, sulfur-containing amino acid, chlorogenic acid, scutelloside etc. to process type Antistress agent of splashing in the patent document of " CN200910156923.5 ", and it can improve the survival rate and the disease-resistant rate of aquaculture organisms.But above-mentioned patent document all introduces the anti-fish body or in breeding water body single occupation mode such as splash, the influence that can improve fish body anti-stress ability of stress preparation throwing something and feeding.The transport stress ability compound formulation aspect patent of relevant raising fish body is not also appeared in the newspapers.Therefore, a kind of preparation method and application that improves fish body transport stress ability compound formulation is provided, helps to improve the survival rate of fish body, improve culturist's economic benefit.
Summary of the invention
The objective of the invention is to overcome the deficiency that exists in the prior art; A kind of compound formulation that improves fish body transport stress ability is provided; It has advantages such as energy consumption is low, technology is simple, production cost is low, can improve fish body transportation anti-stress ability, increases survival rate.
According to technical scheme provided by the invention: improve compound formulation and the preparation method and the application of fish body transport stress ability, it is characterized in that: comprise tryptophan, nucleotides, the fruit of Chinese magnoliavine, Tea Polyphenols, vitamin and carrier, each set of dispense is than meter by weight:
Tryptophan, 40-60 part; Nucleotides, 4-8 part; The fruit of Chinese magnoliavine, 20-30 part; Tea Polyphenols, 0.5-1.5 part; Vitamin E, 1-3 part; Carrier, 14.5-17.5 part.
Said tryptophan is the L-tryptophan, is provided by the purple thinkling sound in Nantong Chemical Industry Science Co., Ltd.Said nucleotides is provided by the Jiangsu Province SuWei microorganism Research Co., Ltd.The said fruit of Chinese magnoliavine is provided by the four seas, Nantong plant essence Co., Ltd.Said Tea Polyphenols is provided by Sheng Kang source, land scientific and technological development Co., Ltd.Said vitamin E is 40% Vitwas E, and worker pharmaceutical Co. Ltd provides by east, Zhejiang.Said carrier is a zeolite powder.
Improve compound formulation and the preparation method and the application of fish body transport stress ability, it is characterized in that: adopt following processing step:
(1) mixing: get tryptophan, nucleotides, the fruit of Chinese magnoliavine, Tea Polyphenols, vitamin E and carrier,, obtain compound with the abundant mixing of above-mentioned each component;
(2) pulverize: the compound behind the abundant mixing is pulverized, processed the compound formulation of 100-120 order dry powder.
Mixer is adopted in the operation of described mixing, and the mixer rotating speed that feeds intake is 18-20 rev/min.
Described crushing operation adopts micronizer to pulverize, and pulverizing rotating speed is 400-500 rev/min.
Improve compound formulation and the preparation method and the application of fish body transport stress ability, it is characterized in that: use in the breeding water body of said compound formulation before the transportation of fish body and in the transportation water body in the transportation, working concentration is 10-20ppm.
Said compound formulation also can be used in the fish meal before the fish body transportation, percentage meter by weight, and the addition of compound formulation is 2%-5%.
Described compound formulation can be used for conventional freshwater fish transportation such as carp, crucian, megalobrama amblycephala; Can directly remove the free radical in the body, accelerate to repair ruined immune system, slow down the reaction of body stressed condition; Can improve fish body transport stress ability, increase survival rate.
Compared with present technology the present invention has the following advantages:
1, the present invention prepares compound formulation with tryptophan, nucleotides, the fruit of Chinese magnoliavine, Tea Polyphenols and vitamin, and that compound formulation has is anti-oxidant, anti-ly stress wait function, can improve fish body transport stress ability, increases survival rate.
2, the present invention from the fish body stress the mechanism angle, in compound formulation, replenished anti-stress Chinese herbal medicine, replenished again stress the time needs such as amino acid, raising fish body transport stress ability.
3, each component raw material steady sources in the compound formulation of the present invention, preparation is simple, draws materials extensively, and is with low cost.
4, the zeolite powder in the compound formulation of the present invention can adsorb some harmful substances of discharging in the fish body transportation, strengthens fish body transport stress ability, increases survival rate.
The specific embodiment
Below in conjunction with specific embodiment the present invention is described further.
Embodiment 1:
The preparation compound formulation:
Proportioning is taken off and is stated component by weight: 14.5 parts of 60 parts of tryptophans, 4 parts in nucleotides, 20 parts in the fruit of Chinese magnoliavine, 0.5 part of Tea Polyphenols, 1 part of vitamin E and zeolite powders; Above-mentioned each component abundant mixing in the mixer (V-Mixer, Liyang Huida Machinery Co., Ltd.), the mixer rotating speed that feeds intake: 18 rev/mins; In micronizer (the small-sized micronizer of ZNC-300, Beijing sharp and development in science and technology Co., Ltd of fashionable), pulverize then, micronizer is pulverized speed: 400 rev/mins; Process compound formulation through 100 purpose screen clothes at last.
The grouping comparison test:
Test divides two groups, and one group is control group, does not add this compound formulation; Another group is test group, and the fish body transports preceding 7 days bold and vigorous these compound formulations of 10ppm in full pond at a distance from 1 day whenever, and in fish meal, adds this compound formulation of 2% (weight) every day, in transport case, adds this compound formulation of 10ppm during transportation.
These two groups of fish bodies all are to transport through the oxygenation of running water car oxygenation gas cylinder.The mixed that the transportation water is pressed 4:1 by natural well water and former pond water, water temperature remains on 10 ℃, and the too high ice cream that adds of water temperature is lowered the temperature.1.1 meters * 1.1 meters * 1.1 meters of fortune fish and water case specifications, megalobrama amblycephala 450 kg about 50g are placed into fortune fish and water case, 9000 of transportation fish bodies, road distance 300 km.Control group is dead 512 as a result, survival rate 94.31%; Test group is dead 178, survival rate 98.02%.
Can find out that by above-mentioned contrast test the compound formulation of the embodiment of the invention 1 preparation can improve fish body transport stress ability, improves survival rate.
Embodiment 2:
The preparation compound formulation:
Proportioning is taken off and is stated component by weight: 16 parts of 50 parts of tryptophans, 6 parts in nucleotides, 25 parts in the fruit of Chinese magnoliavine, 1 part of Tea Polyphenols, 2 parts of vitamin Es and zeolite powders; Above-mentioned each component abundant mixing in the mixer (V-Mixer, Liyang Huida Machinery Co., Ltd.), the mixer rotating speed that feeds intake: 19 rev/mins; In micronizer (the small-sized micronizer of ZNC-300, Beijing sharp and development in science and technology Co., Ltd of fashionable), pulverize then, micronizer is pulverized speed: 500 rev/mins; Process compound formulation through 110 purpose screen clothes at last.
The grouping comparison test:
Test divides two groups, and one group is control group, does not add this compound formulation; Another group is test group, and the fish body transports preceding 7 days bold and vigorous these compound formulations of 15ppm in full pond at a distance from 1 day whenever, and in fish meal, adds this compound formulation of 3% (weight) every day, in transport case, adds this compound formulation of 15ppm during transportation.
These two groups of fish bodies all are to transport through the oxygenation of running water car oxygenation gas cylinder.The transportation water is pressed the 4:1 mixed by natural well water and former pond water, and water temperature remains on 10 ℃, and the too high ice cream that adds of water temperature is lowered the temperature.1.1 meters * 1.1 meters * 1.1 meters of fortune fish and water case specifications, megalobrama amblycephala 500 kg about 500g are placed into fortune fish and water case, 1000 of transportation fish bodies, road distance 200 km.Control group is dead 56 as a result, survival rate 94.40%; Test group is dead 2, survival rate 99.80%.
Can find out that by above-mentioned contrast test the compound formulation of the embodiment of the invention 2 preparations can improve fish body transport stress ability, improves survival rate.
Embodiment 3:
The preparation compound formulation:
Proportioning is taken off and is stated component by weight: 17.5 parts of 40 parts of tryptophans, 8 parts in nucleotides, 30 parts in the fruit of Chinese magnoliavine, 1.5 parts of Tea Polyphenols, 3 parts of vitamin Es and zeolite powders; Above-mentioned each component abundant mixing in the mixer (V-Mixer, Liyang Huida Machinery Co., Ltd.), the mixer rotating speed that feeds intake: 20 rev/mins; In micronizer (the small-sized micronizer of ZNC-300, Beijing sharp and development in science and technology Co., Ltd of fashionable), pulverize then, micronizer is pulverized speed: 450 rev/mins; Process compound formulation through 120 purpose screen clothes at last.
The grouping comparison test:
Test divides two groups, and one group is control group, does not add this compound formulation; Another group is test group, and the fish body transports preceding 7 days bold and vigorous these compound formulations of 20ppm in full pond at a distance from 1 day whenever, and in fish meal, adds this compound formulation of 5% (weight) every day, in transport case, adds this compound formulation of 20ppm during transportation.
These two groups of fish bodies all are to transport through the oxygenation of running water car oxygenation gas cylinder.The transportation water is pressed the 4:1 mixed by natural well water and former pond water, and water temperature remains on 10 ℃, and the too high ice cream that adds of water temperature is lowered the temperature.1.1 meters * 1.1 meters * 1.1 meters of fortune fish and water case specifications, megalobrama amblycephala 400 kg about 2g are placed into fortune fish and water case, 20000 of transportation fish bodies, road distance 100 km.Control group is dead 1934 as a result, survival rate 90.33%; Test group is dead 523, survival rate 97.39%.
Can find out that by above-mentioned contrast test the compound formulation of the embodiment of the invention 3 preparations can improve fish body transport stress ability, improves survival rate.
Claims (9)
1. improve the compound formulation of fish body transport stress ability, it is characterized in that: comprise tryptophan, nucleotides, the fruit of Chinese magnoliavine, Tea Polyphenols, vitamin and carrier, each set of dispense is than counting by weight:
Tryptophan, 40-60 part; Nucleotides, 4-8 part; The fruit of Chinese magnoliavine, 20-30 part; Tea Polyphenols, 0.5-1.5 part; Vitamin E, 1-3 part; Carrier, 14.5-17.5 part.
2. the compound formulation of raising fish body transport stress ability as claimed in claim 1, it is characterized in that: said tryptophan is the L-tryptophan.
3. the compound formulation of raising fish body transport stress ability as claimed in claim 1, it is characterized in that: said vitamin E is 40% Vitwas E.
4. the compound formulation of raising fish body transport stress ability as claimed in claim 1, it is characterized in that: said carrier is a zeolite powder.
5. improve the preparation method of the compound formulation of fish body transport stress ability, it is characterized in that: adopt following processing step:
(1) mixing: get tryptophan, nucleotides, the fruit of Chinese magnoliavine, Tea Polyphenols, vitamin E and carrier,, obtain compound with the abundant mixing of above-mentioned each component;
(2) pulverize: the compound behind the abundant mixing is pulverized, processed the compound formulation of 100-120 order dry powder.
6. the preparation method of the compound formulation of raising as claimed in claim 5 fish body transport stress ability is characterized in that: mixer is adopted in described mixing operation, and the mixer rotating speed that feeds intake is 18-20 rev/min.
7. the preparation method of the compound formulation of raising fish body transport stress ability as claimed in claim 5, it is characterized in that: described crushing operation adopts micronizer to pulverize, and pulverizing rotating speed is 400-500 rev/min.
8. improve the application of the compound formulation of fish body transport stress ability, it is characterized in that: use in the breeding water body of said compound formulation before the transportation of fish body and in the transportation water body in the transportation, working concentration is 10-20ppm.
9. the application of the compound formulation of raising as claimed in claim 8 fish body transport stress ability is characterized in that: said compound formulation also can be used in the fish meal before the transportation of fish body, percentage meter by weight, and the addition of compound formulation is 2%-5%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011103949749A CN102406091B (en) | 2011-12-02 | 2011-12-02 | Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof |
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| CN2011103949749A CN102406091B (en) | 2011-12-02 | 2011-12-02 | Composite preparation for improving fish body transportation stress capability as well as preparation method and application thereof |
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Cited By (6)
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| CN103190364A (en) * | 2013-03-29 | 2013-07-10 | 中国水产科学研究院长江水产研究所 | Method and injection liquid for nursing wild coreius guichenoti |
| CN103299939A (en) * | 2013-06-28 | 2013-09-18 | 青岛农业大学 | Method for removing heavy metal cadmium from clam |
| CN105076877A (en) * | 2015-09-17 | 2015-11-25 | 通威股份有限公司 | Schizothorax prenanti parent fish feed |
| CN105851523A (en) * | 2016-04-06 | 2016-08-17 | 宁波大学 | Stress resistance reinforcing agent for harvestfishes and application of stress resistance reinforcing agent |
| CN111011270A (en) * | 2019-12-05 | 2020-04-17 | 天津农学院 | Method for relieving fish transportation stress and application |
| CN113519417A (en) * | 2021-07-14 | 2021-10-22 | 中国水产科学研究院黄海水产研究所 | Luhai relay breeding method for oplegnathus punctatus |
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| CN101357223A (en) * | 2008-08-30 | 2009-02-04 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of anti-stress agent for fishing |
| CN101480220A (en) * | 2008-11-07 | 2009-07-15 | 江苏省苏微微生物研究有限公司 | Method for preparing mixed type nucleotide feed addictive from yeast cell and application |
| CN101941938A (en) * | 2010-08-27 | 2011-01-12 | 长沙兴嘉生物工程股份有限公司 | Tryptophan chelate and preparation method and application thereof |
| CN102178065A (en) * | 2011-05-31 | 2011-09-14 | 中国科学院东北地理与农业生态研究所 | Anti-stress feed with humic acid |
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- 2011-12-02 CN CN2011103949749A patent/CN102406091B/en not_active Expired - Fee Related
Patent Citations (4)
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| CN101357223A (en) * | 2008-08-30 | 2009-02-04 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of anti-stress agent for fishing |
| CN101480220A (en) * | 2008-11-07 | 2009-07-15 | 江苏省苏微微生物研究有限公司 | Method for preparing mixed type nucleotide feed addictive from yeast cell and application |
| CN101941938A (en) * | 2010-08-27 | 2011-01-12 | 长沙兴嘉生物工程股份有限公司 | Tryptophan chelate and preparation method and application thereof |
| CN102178065A (en) * | 2011-05-31 | 2011-09-14 | 中国科学院东北地理与农业生态研究所 | Anti-stress feed with humic acid |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103190364A (en) * | 2013-03-29 | 2013-07-10 | 中国水产科学研究院长江水产研究所 | Method and injection liquid for nursing wild coreius guichenoti |
| CN103299939A (en) * | 2013-06-28 | 2013-09-18 | 青岛农业大学 | Method for removing heavy metal cadmium from clam |
| CN103299939B (en) * | 2013-06-28 | 2015-02-11 | 青岛农业大学 | Method for removing heavy metal cadmium from clam |
| CN105076877A (en) * | 2015-09-17 | 2015-11-25 | 通威股份有限公司 | Schizothorax prenanti parent fish feed |
| CN105851523A (en) * | 2016-04-06 | 2016-08-17 | 宁波大学 | Stress resistance reinforcing agent for harvestfishes and application of stress resistance reinforcing agent |
| CN111011270A (en) * | 2019-12-05 | 2020-04-17 | 天津农学院 | Method for relieving fish transportation stress and application |
| CN113519417A (en) * | 2021-07-14 | 2021-10-22 | 中国水产科学研究院黄海水产研究所 | Luhai relay breeding method for oplegnathus punctatus |
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| CN102406091B (en) | 2013-03-20 |
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