CN102405955B

CN102405955B - Frozen storage method of globefish

Info

Publication number: CN102405955B
Application number: CN 201110351393
Authority: CN
Inventors: 谢晶; 马妍; 王金锋; 刘骁
Original assignee: Shanghai Maritime University
Current assignee: Shanghai Maritime University; Shanghai Ocean University
Priority date: 2011-11-09
Filing date: 2011-11-09
Publication date: 2013-04-03
Anticipated expiration: 2031-11-09
Also published as: CN102405955A

Abstract

The invention discloses a frozen storage method of globefish. The method comprises the following specific steps of: 1, collecting healthy globefishes which are uniform in size, and getting live globefishes back; 2, preparing a certain amount of antifreeze agent solution; 3, slaughtering the live globefishes, removing heads and viscera, chopping into blocks, and washing with sterile water; 4, quickly freezing fish blocks in a refrigerator at 18 DEG C below zero; and 5, taking the frozen fish blocks out, soaking the fish blocks in the low-temperature antifreeze agent solution, and bagging the fish blocks when the antifreeze agent solution is iced on the surfaces of the fish blocks to form a thick ice coat, and finally storing and freezing the bagged fish blocks in the refrigerator at 25 DEG C below zero. The formula of the antifreeze agent comprises the following components: 4.5-5.5% of trehalose, 0.3-0.5% of sodium polyphosphate, 0.03-0.07% of epsilon-polylysine and the balance of distilled water. The method plays a role in inhibiting degeneration of proteins of globefishes to a certain extent, has no side effect on a human body, is simple to operate and is safe and environmentally-friendly.

Description

A kind of method of globe fish cold storage

Technical field

The present invention relates to the food and medicine processing technique field, relate in particular to the antifreeze preparation field that is used for the CKIs qualitative change in the chilled storage, be specifically related to a kind of preparation that suppresses the antifreeze ice clothing of globe fish fish meat protein sex change in the chilled storage process.

Background technology

Globe fish (puffer fish) has another name called Puffer, is a kind of fish that a kind of internal organ contain tetraodotoxin, and up to a hundred kinds are arranged.Although the tetraodotoxin toxicity in the filefish is very strong, but its delicious flavour, meat is fine and smooth, contains rich in protein and unsaturated fat, especially be rich in the pentacosa alkene acid (EPA) and the DHA (DHA) that are called as " docosapentaenoic acid ", have high nutritive value.The globe fish resource of China is very abundant, and the globe fish that originates in China has more than 40 to plant, and has become the in the world production and marketing big country of globe fish.But owing to the distinctive biology of globe fish itself, chemical characteristic, water content is high, and is as easy as rolling off a log putrid and deteriorated, thereby makes the flesh quality reduction or lose edible and nutritive value, so the preservation of globe fish becomes people's problems of concern gradually.In the freezing process, affect mouthfeel owing to the phenomenon such as freeze denaturation can occur for the protein of the flesh of fish, the antifreeze ice clothing prescription of therefore seeking a kind of safety and sanitation and can significantly suppressing globe fish meat protein freeze denaturation seems very important.

As far back as nineteen fifty-nine Japanese scholars the low-temperature protection effect of sucrose to frozen minced fillets at first proposed, this is because carbohydrate can come indirectly protein to be shielded by the state and the character that change existing water in the fish protein.At present, the antifreeze of commonly using on the market has sucrose, sorbierite, composite phosphate and lecithin, sucrose ester etc.But because the interpolation of sucrose and sorbierite etc. can cause the sugariness of product to increase, affected the special favor of product itself, therefore study and a kind ofly can guarantee product quality, the antifreeze ice clothing prescription that can reach again protein denaturation in the remarkable inhibition globe fish refrigerating process is a problem that waits to solve.

Summary of the invention

Globe fish in during frozen storage, its salting-in protein content, sulfhydryl content, fribrillin Ca ²⁺A series of variation can occur in-atpase activity, VBN (TVB-N), pH value and juice loss rate.The object of the invention is to by to its salting-in protein content, sulfhydryl content, fribrillin Ca ²⁺-atpase activity, VBN (TVB-N), pH value and juice loss rate are estimated, provide a kind of and can farthest keep the original local flavor of globe fish, but the antifreeze of establishment fish meat protein sex change in long-term freezing storage process ice clothing prescription again is for the chilled storage of globe fish provides certain technical support.

Trehalose in the antifreeze that the present invention adopts is the Novel anti-freezing agent of just using in food processing in recent years, it has the age of starch of preventing, prevents protein denaturation, suppresses the generation of rotten fishy smell, stink, the stabilizing tissue eucaryotic cell structure, the functional characteristics such as flavoring and the decomposition of inhibition aliphatic acid.Trehalose is with α, α, and the irreducibility disaccharide that 1,1 glycosidic bond connects, its Antifreezing Mechanism may be similar to common carbohydrate.But trehalose has more superior biochemical protective effect and stability than general carbohydrate; because trehalose has high glass transition temperature; comprise less free volume, restricted molecular mobility and in storage opposing be separated and the ability of crystallization; and its sugariness is 45% of sucrose, and the characteristics of its low sugariness more can embody the original flavor of food in food processing.

Sodium polyphosphate is one of most widely used food additives in present countries in the world, it is widely used in the every field of food production, improvement to food quality plays an important role, can prevent and reduce the oxidation that aquatic products occur in processing and storage process, reduce the human body because of oxidation variable color, spoiled, make its muscle groups be woven with better moisture holding capacity, local flavor is better and improve its retentiveness when thawing.In addition, phosphate also has emulsification, prevents that protein, fat from separating, and increases caking property, improves the institutional framework of mixture, makes and organizes soft and succulency.

Polylysine was found by Japanese scientist in 1977 the earliest, was produced by streptomycete, and it is by the lysine monomer composition, and entering behind the human body can complete digested absorption, not only without any toxic and side effect, and can be used as a kind of source of lysine.Epsilon-polylysine is at present generally as food preservative, is used for food fresh keeping, have wide spectrum, efficient, nontoxic, be subjected to the pH value to affect the characteristics such as little.

Suppress the antifreeze ice clothing preparation method of globe fish meat protein sex change in the during frozen storage, concrete steps are as follows:

1. gather the adult globe fish (every treaty 500g) of big or small homogeneous health, live body is transported back, adopts the method for oxygenation keep-alive in transportation;

2. first trehalose is added in the distilled water with mass ratio 4.5～5.5%, then sodium polyphosphate is added in the distilled water with mass ratio 0.3～0.5%, at last epsilon-polylysine is dissolved in the distilled water with mass ratio 0.03～0.07%, stirred 1 minute, reach even, the clarification of assurance solution without precipitation evenly, places 2 ℃ of refrigerator low temperature to preserve.

3. after the globe fish live body is slaughtered, remove head and internal organ, piecemeal is used aseptic water washing 2 times;

4. the flesh of fish is put into-18 ℃ of refrigerator quick-frozens 5-15 minute;

5. freeze rear taking-up until the flesh of fish, immerse 15-30 second in the Cryoprotectant solution, treat that antifreeze solution forms thick layer ice clothing on fish piece surface, during ice clothing thickness 4-6mm, place-25 ℃ of refrigerator and cooled to freeze storage in the packaged bag of fish.

Concrete globe fish operating procedure is:

1. gather the adult globe fish of every treaty 500g size homogeneous health, live body is transported back, oxygenation keep-alive in transportation;

2. trehalose, sodium polyphosphate and epsilon-polylysine are added in the distilled water with 4.5～5.5%, 0.3～0.5% and 0.03～0.07% mass ratio respectively, stirred 1 minute, reach evenly, place 2 ℃ of refrigerator low temperature preservations;

3. after the globe fish live body is slaughtered, remove head and internal organ, piecemeal is used aseptic water washing;

Description of drawings

Fig. 1 is the change curve of the hydroxy radical content of globe fish after processing in the specific embodiment and control group;

Fig. 2 is the change curve of the pH value of globe fish after processing in the specific embodiment and control group;

Fig. 3 is the change curve of the salting-in protein content of globe fish after processing in the specific embodiment and control group;

Fig. 4 is the change curve of the VBN of globe fish after processing in the specific embodiment and control group.

The specific embodiment

For fully disclosing antifreeze ice clothing prescription of the present invention, be illustrated below in conjunction with embodiment.

Embodiment:

(1) the adult globe fish (every treaty 500g) of the big or small homogeneous health of collection, keep-alive is transported back;

(2) after live body is slaughtered, remove head and internal organ, piecemeal is used aseptic water washing;

(3) flesh of fish is put into-18 ℃ of refrigerator quick-frozens 10 minutes;

(4) freeze rear taking-up until the flesh of fish, immerse in the Cryoprotectant solution 20 seconds time;

(5) treat that antifreeze solution forms thick layer ice clothing on fish piece surface, place-25 ℃ of refrigerator and cooled to freeze storage in the packaged bag of fish.

The preparation of above-mentioned antifreeze solution is grouped into by A, B, three kinds of one-tenth of C, and wherein A is that trehalose, B are that sodium polyphosphate, C are epsilon-polylysine.They are respectively the trehalose mass percent at shared ratio: 5.0%; Sodium polyphosphate mass percent: 0.4%; The epsilon-polylysine mass percent: 0.05%, all the other are prepared from for distilled water.

Trehalose, sodium polyphosphate and epsilon-polylysine respectively with in 5.0%, 0.4% and 0.05% the mass ratio adding distilled water, were stirred 1 minute, reach evenly, guarantee the solution clarification, without precipitating, place 2 ℃ of refrigerator low temperature to preserve.The globe fish that piecemeal is good is put into first-18 ℃ of refrigerator quick-frozens 10 minutes, takes out after being frozen into piece, adds in the Cryoprotectant solution of being prepared with 1: 1 mass ratio, soaks 20 seconds, forms until fish piece surface and takes out after thick layer is iced clothing.The globe fish piece of handling well is put into the sterilized antistaling bag, in-25 ℃ of refrigerators, store, and to its carry out salting-in protein content, sulfhydryl content, VBN (TVB-N), pH value with etc. the mensuration of index, wherein control group is for to replace antifreeze with distilled water.

By each figure as can be known, in 180 days storage period, the pH value of the flesh of fish, the variation tendency of VBN, salting-in protein and sulfhydryl content is less than control group, experimental result shows: the globe fish that utilizes antifreeze ice clothing to process, on indices, all be better than control group, obviously suppressed the sex change of fish meat protein, kept good taste quality.

Claims

1. the method for a globe fish cold storage, concrete steps are as follows:

1. gather every 500g, big or small homogeneous, healthy adult globe fish, live body is transported back, takes oxygenation, water feeding method in transportation, guarantees the health status of globe fish;

2. trehalose, sodium polyphosphate and epsilon-polylysine are added in the distilled water with 4.5 ~ 5.5%, 0.3 ~ 0.5% and 0.03 ~ 0.07% mass ratio respectively, stirred 1 minute, reach evenly, make Cryoprotectant solution and place 2 ℃ of refrigerator low temperature preservations;

4. the flesh of fish is put into-18 ℃ of refrigerator quick-frozens 5 ~ 15 minutes;

5. freeze rear taking-up until the flesh of fish, immersed in the antifreeze solution 15 ~ 30 seconds, treat that antifreeze solution forms thick layer ice clothing on fish piece surface, during ice clothing thickness 4 ~ 6mm, place-25 ℃ of refrigerator and cooled to freeze storage in the packaged bag of fish.

2. the method for a kind of globe fish cold storage as claimed in claim 1, it is characterized in that: globe fish is after transporting back, and live body is slaughtered, and removes head and internal organ, be divided into big or small homogeneous, 10g fish piece after, wash 2 times with sterilized water.

3. the method for a kind of globe fish cold storage as claimed in claim 1 is characterized in that: cleaned fish piece is drained, place-18 ℃ of refrigerator quick-frozens 10 minutes, wait freezing rear taking-up.

4. the method for a kind of globe fish cold storage as claimed in claim 1, it is characterized in that: described antifreeze is formulated by trehalose, sodium polyphosphate and epsilon-polylysine, wherein the trehalose mass percent: 5.0 %; Sodium polyphosphate mass percent: 0.4 %; The epsilon-polylysine mass percent: 0.05 %, all the other are prepared from for distilled water, and antifreeze solution dissolves evenly, and clarification without precipitation, is positioned over low temperature preservation in 2 ℃ of refrigerators after making.

5. the method for a kind of globe fish cold storage as claimed in claim 1 is characterized in that: globe fish is mixed with the mass ratio of antifreeze solution by 1:1, soak 20s, treat that fish piece surface forms one deck ice clothing, thickness takes out after reaching 5mm.

6. the method for a kind of globe fish cold storage as claimed in claim 1 is characterized in that: the globe fish piece of handling well, put in the sterile food freshness protection package, and place-25 ℃ of refrigerator and cooled to freeze storage.