CN103564617A - Micro-ice crystal freeze-preservation freezing aid for animals and plants and application method thereof - Google Patents

Micro-ice crystal freeze-preservation freezing aid for animals and plants and application method thereof Download PDF

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Publication number
CN103564617A
CN103564617A CN201310430654.3A CN201310430654A CN103564617A CN 103564617 A CN103564617 A CN 103564617A CN 201310430654 A CN201310430654 A CN 201310430654A CN 103564617 A CN103564617 A CN 103564617A
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animal
phosphate
trehalose
ice crystal
agent
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黄雨诗
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/36Freezing; Subsequent thawing; Cooling
    • A23L3/37Freezing; Subsequent thawing; Cooling with addition of or treatment with chemicals
    • A23L3/375Freezing; Subsequent thawing; Cooling with addition of or treatment with chemicals with direct contact between the food and the chemical, e.g. liquid nitrogen, at cryogenic temperature
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
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  • Food Science & Technology (AREA)
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  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention relates to a micro-ice crystal freezing aid, particularly a micro-ice crystal freezing aid for animals and plants and an application method thereof. The freezing aid comprises the following components in percentage by mass: 0.2-5% of trehalose, 0.1-3% of phosphate and the balance of water. The application method comprises the following steps: (1) proportionally preparing trehalose, phosphate and water into the freezing aid; (2) putting a refrigerated substance into the prepared 0-10 DEG C freezing aid to perform precooling immersion for 30-60 minutes; and (3) sending the precooled refrigerated substance into a refrigerator to be refrigerated. The freezing aid can keep the cytomembranes of the frozen animal or plant complete, so that the animal or plant tissues are kept complete, and the ice crystals in the animal or plant tissues are fine; and after long-term freeze preservation, the cytomembranes of the animal or plant can be still kept complete, and the defrozen animal and plant still has the original color, flavor, elasticity for chewing and nutrition.

Description

A kind of animal and plant body micro ice crystal freeze preservation helps and freezes agent and using method
Technical field
The present invention relates to a kind of micro ice crystal freeze preservation and help and freeze agent, particularly a kind of animal and plant body micro ice crystal freeze preservation helps and freezes agent.
Background technology
The freezing processing of animal and plant body at present mainly adopts the method for freezing and refrigeration, the cryogenic freezing of short time freezes to cause somatic breaking, product surface jaundice after long-time refrigeration, after thawing, protein runs off, and makes animal and plant body lose original color and luster, flavour and chew malleable and nutrition.
Summary of the invention
The object of the invention is to: solve in prior art the freezing processing of animal and plant body and mainly adopt the method for freezing and refrigeration when sharp freezing, to produce larger ice crystal to cause body cell to break, product surface jaundice after long-time refrigeration, after thawing, protein runs off, and makes product lose the problem of original delicate flavour and nutrition.
The present invention is achieved through the following technical solutions: of the present invention helping frozen agent and comprised trehalose, phosphate;
Wherein trehalose mass fraction is 0.2%-5%, and phosphate mass fraction is 0.1-3%, and surplus is water.
Animal and plant body of the present invention helps the using method of freezing agent, following three steps, consists of:
(1) preparation helps and freezes agent: according to phosphate mass fraction, be 0.1~3%, trehalose mass fraction is 0.2%~5%, and the proportioning mode that surplus is water joins phosphate and trehalose in the food-grade water that meets food hygiene requirement, is mixed with to help and freezes liquid;
(2) precooling technique: the article that need cold storing and fresh-keeping are removed to impurity and wash down after put into 0 ℃ for preparing in advance--liquid is frozen in 10 ℃ help, at 0 ℃--at the temperature of 10 ℃, precooling is soaked, and the time is 10~60 minutes;
(3) warehouse-in refrigeration: drain away the water, will refrigerate after the good food product refrigeration of precooling.
When need to thaw, only fish body need be immersed in completely in the appropriate saline solution of concentration and soak and thaw, wherein the optium concentration of saline solution is 1%-3%, and temperature is at 5 ℃--10 ℃.
Beneficial effect of the present invention is: can make the cell membrane of freezing animal and plant body keep complete, animal and plant body tissue remains intact, in animal and plant body tissue, ice crystal is trickle, the cell membrane that Long-term Cold Storage is preserved rear animal and plant body still can guarantee complete, animal and plant body is organized still and is remained intact, in animal and plant body tissue, ice crystal is still tiny, and after thawing, animal and plant body keeps original color and luster, flavour and chews malleable and nutrition.
Accompanying drawing explanation
Fig. 1 is the rheum officinale flesh of fish section micro-image after adopting traditional refrigerating process freezing;
Fig. 2 processes the rheum officinale flesh of fish section micro-image after freezing for adopting existing other to help to freeze liquid;
Fig. 3 is the rheum officinale flesh of fish section micro-image after power-assist liquid of the present invention is processed and is freezing.
The specific embodiment
Of the present invention helping frozen agent and comprised trehalose, phosphate;
Wherein trehalose mass fraction is 0.2%~5%(but must not surpasses 5%, while surpassing 5%, precooling thing can be with sweet sense, make product not possess genuineness), phosphate mass fraction is 0.1~3%(phosphate may affect product effect lower than 0.1% time, solution can produce precipitation, phosphate content and higher than 3%, also easily make food produce peculiar smell to make product not possess genuineness because forming saturated solution higher than 2.5% time), surplus is water.
Described help that to freeze the optimal value of agent each component as follows:
Trehalose mass fraction is 1%, and phosphate mass fraction is 2%, and surplus is water.
Animal and plant body of the present invention helps the using method of freezing agent, following three steps, consists of:
(1) preparation helps and freezes agent: according to phosphate mass fraction, be 0.1~3%, trehalose mass fraction is 0.2%~5%, and the proportioning mode that surplus is water joins phosphate and trehalose in the drinking water that meets food hygiene requirement, is mixed with to help and freezes liquid;
(2) precooling technique: the article that need cold storing and fresh-keeping are removed to impurity and wash down after put into 0 ℃ for preparing in advance--liquid is frozen in 10 ℃ help, at 0 ℃--at the temperature of 10 ℃, precooling is soaked, and the time is 10~60 minutes;
(3) warehouse-in refrigeration: drain away the water, will refrigerate after the good food product refrigeration of precooling.
Phosphate have maintain animal and plant body water holding capacity, suppress and the mixture that prevents animal and plant body internal protein freeze denaturation ability such as: sodium dihydrogen phosphate, potassium dihydrogen phosphate, disodium hydrogen phosphate, dikalium phosphate, tertiary sodium phosphate, tripotassium phosphate, sodium pyrophosphate, potassium pyrophosphate, sodium polyphosphate, potassium polyphosphate or 2 kinds or two or more these material etc. all has above-mentioned functions.
Phosphate of the present invention comprises all phosphate and 2 kinds or above phosphate mixt.
Embodiment 1:
1, freezing the helping of configuration micro ice crystal frozen liquid: take 1.4 kilograms of phosphate, trehalose: 0.7 kilogram.Phosphate and trehalose are dissolved in 97.9 kilograms, 0 ℃~10 ℃ running water (or drinking water of foodstuff sanitation-stage) completely.
2, precooling technique: the large yellow croaker after cleaning (removing the large yellow croaker after surface impurity) is put into the micro ice crystal of 0 ℃~10 ℃ configuring and help and freeze liquid precooling, (fish and help the weight ratio of freezing liquid to be about 1:1.5, help and freeze liquid measure while being less than 1.5 times of fish weight, fish body precooling cooling-down effect is poor; Help and freeze liquid usage ratio and greatly affect cost.) precooling soak time is about 30 minutes.
3, warehouse-in refrigeration: drain away the water, the good refrigeration thing of precooling is sent into the freezing and refrigeration of freezer.
Through this method, help the large yellow croaker cell membrane freezing after processing complete, animal and plant body tissue remains intact, and color and luster remains intact, and has reached fresh-keeping effect.Again after thawing, can reach the similar fragrance of fresh large yellow croaker, flavour and chew strength.
Note: 1, according to the size of fish body, the above-mentioned interpolation concentration of freezing agent that helps can make the appropriate adjustments.
2, the fish of formed objects, helps and freezes liquid concentration as changed, precooling time lengthening or shorten and also can reach propinquity effect.
3, precooling soaking temperature is generally set in 0 ℃~10 ℃, but, if helped, freeze liquid and precooling food cell inside and outside (or in article) liquid and do not freeze, precooling temperature can be lower than 0 ℃, if can not there is the reasons such as microorganism, do not cause food spoilage phenomenon, if precooling soaking temperature also can surpass the large or epidermis of 10 ℃ of volume of food that need freeze preservation or fat deposit is thicker, suitably control precooling soaking temperature and keep reduced levels, guaranteeing under food stay-in-grade prerequisite, pre-cool time can be over 60 minutes.If the fish that precooling individuality is less, shrimps are during as dried shrimp or cloves fish, precooling soak time also can be less than 10 minutes.
4, freezing and refrigeration refrigerates after can adopting all kinds of quick-frozen modes (dull and stereotyped quick-frozen or spiral quick-frozen and additive method quick-frozen).Individual product also can be without quick-frozen, directly enter the freezing preservation of freezer.
5, use the freezing product of micro ice crystal refrigeration fresh-keeping technique when thawing, to make better processing effect with the following methods:
(1), configuration thawing solution, thawing solution is the saline solution of concentration 1%--3%;
(2), fish being put into thawing solution immersion thaws.The liquid measure of thawing is the least possible, does not just have fish surface for good.Thawing time summer (approximately 25 ℃ of left and right of environment temperature) approximately needed about 4 hours, and winter, (approximately 10 ℃ of left and right of environment temperature) approximately needed about 8 hours.
According to chilled index, evaluate the following parameter of comparative evaluation, whether flake is limpid full, the fish gill whether color and luster, fish body surface whether color and luster, fish body touch by elasticity, oppress whether flavour, the flesh of fish and whether chew strength, chilled whole indexs are all 9 minutes, and the index of the acquisition in embodiment 1 is respectively 7 minutes, 7 minutes, 8 minutes, 7 minutes, 7 minutes.
Embodiment 2-9 and comparative example 1-11
On the basis of embodiment 1, replace freezing composition and concentration, precooling soak time and the precooling soaking temperature that freezes component in liquid that help of micro ice crystal, other conditions are constant, and adopt identical assay method and index, obtain corresponding correction data, the replacement situation of each parameter and quality evaluation result difference reference table 1 and table 2 in subsequent embodiment.
Table 1:
Figure BDA0000384421640000031
Table 2
Table 2 remarks: the flake of chilled large yellow croaker of take is limpid, full is 9 minutes; Fish gill color and luster is scarlet, viscous liquid state is 9 minutes; Not 9 minutes, fish body colour pool; It is 9 minutes by elasticity that fish body touches; Flesh of fish flavour refers to that the flavour after culinary art (steaming in clear soup) is 9 minutes; The flesh of fish is chewed strength and is referred to that chewing after culinary art (steaming in clear soup) is 9 minutes ,-with the These parameters of chilled large yellow croaker, pass judgment on each These parameters of organizing of A-S.
Table 1 table 2 is found after contrast:
1, the formula rate of embodiment 4 and soak time are best.
When 2, trehalose component ratio reaches 4%, oppress flavour and be subject to minimal effect;
When 3, trehalose component reaches 5% over 4%, oppress flavour slightly pleasantly sweet, affected genuineness.
4, be applied in large yellow croaker freeze preservation, soak time 30 minutes is best, and more than 30 minutes to 40 minute effect does not change.
5, polyphosphate component is 2%, and when trehalose component reaches 3% over 1%, indices is basically identical;
6, the system of D-sorbite+trehalose is not so good as the fresh-keeping effect of polyphosphate+trehalose system to the fresh-keeping effect of fish;
7, the fresh-keeping effect of polyphosphate+D-sorbite+trehalose and the fresh-keeping effect of polyphosphate+trehalose are basically identical;
When 8, the component of trehalose+D-sorbite is too high, can affect flavour after fish culinary art (in component the content height of sugar can affect flavour) content of polyphosphate meets or exceeds 3% and understands the flavour that affect fish.
In conjunction with picture, effect of the present invention is described
Picture amplifies 1000 times of slice maps (pink is structure of fish muscle, and white is ice crystal) for produce the flesh of fish in large yellow croaker body for different process:
Fig. 1 is the flesh of fish section of traditional refrigerating process, and as can be seen from the figure large, the structure of fish muscle of ice crystal is substantially by ice crystal (directly) cut-out, and preservation condition is poor;
Fig. 2 is the flesh of fish section of processing through a kind of existing freezing auxiliary agent, and as can be seen from the figure ice crystal is with respect to traditional handicraft, and volume reduces to some extent, and the preservation condition of structure of fish muscle makes moderate progress, but still has the fracture phenomena of structure of fish muscle;
The flesh of fish section of Fig. 3 for processing through the present invention, as can be seen from the figure has significant progress compared with traditional handicraft and existing freezing auxiliary agent treatment technology, and ice crystal is small, and flesh of fish cell keeps complete.

Claims (3)

1. animal and plant body micro ice crystal freeze preservation helps and freezes an agent, it is characterized in that: described helping frozen agent and comprised trehalose, phosphate; Wherein trehalose mass fraction is 0.2%~5%, and phosphate mass fraction is 0.1~3%, and surplus is water.
2. a kind of animal and plant body micro ice crystal freeze preservation according to claim 1 helps and freezes agent, it is characterized in that: the optimal value that agent each component is frozen in described helping is as follows: trehalose mass fraction is 1%, and phosphate mass fraction is 2%, and surplus is water.
3. animal and plant body micro ice crystal freeze preservation according to claim 1 helps the using method of freezing agent, it is characterized in that: following three steps, consist of:
(1) preparation helps and freezes agent: according to phosphate mass fraction, be 0.1~3%, trehalose mass fraction is 0.2%~5%, and the proportioning mode that surplus is water joins phosphate and trehalose in the food-grade water that meets food hygiene requirement, is mixed with to help and freezes liquid;
(2) precooling technique: the article that need cold storing and fresh-keeping are removed to impurity and wash down after put into 0 ℃ for preparing in advance--liquid is frozen in 10 ℃ help, at 0 ℃--at the temperature of 10 ℃, precooling is soaked, and the time is 10~60 minutes;
(3) warehouse-in refrigeration: drain away the water, will refrigerate after the good food product refrigeration of precooling.
CN201310430654.3A 2013-09-18 2013-09-18 Micro-ice crystal freeze-preservation freezing aid for animals and plants and application method thereof Pending CN103564617A (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106107415A (en) * 2016-09-14 2016-11-16 北海宏泰水产有限公司 A kind of frozen dose and application process changing food Glass transition temperature in quick-freezing
EP3354137A4 (en) * 2015-09-22 2019-03-13 Huang, Xiang Animal, plant, fruit and vegetable freezing pretreatment method
CN109567067A (en) * 2018-10-30 2019-04-05 浙江工业大学 A kind of quality holding cooking processing method of the flesh of fish
CN110547323A (en) * 2019-06-28 2019-12-10 广东省农业科学院蚕业与农产品加工研究所 Formula for protecting eyes of frozen fish and using method thereof
CN110800797A (en) * 2019-11-27 2020-02-18 浙江省海洋水产研究所 Micro-freezing preservation method for swimming crabs
CN111000168A (en) * 2019-12-19 2020-04-14 武汉良之隆食材股份有限公司 Processing method of ice mountain mussel capable of being used as thorn body
CN113229353A (en) * 2021-06-02 2021-08-10 渤海大学 Preparation and thawing method of frozen pear dices thawing solution
CN114431283A (en) * 2022-02-22 2022-05-06 集美大学 Fish composite antifreeze agent and application thereof

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CN101664052A (en) * 2009-09-08 2010-03-10 中国农业大学 Low temperature denaturing protective agent of minced fillet of grass carp

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3354137A4 (en) * 2015-09-22 2019-03-13 Huang, Xiang Animal, plant, fruit and vegetable freezing pretreatment method
CN106107415A (en) * 2016-09-14 2016-11-16 北海宏泰水产有限公司 A kind of frozen dose and application process changing food Glass transition temperature in quick-freezing
CN109567067A (en) * 2018-10-30 2019-04-05 浙江工业大学 A kind of quality holding cooking processing method of the flesh of fish
CN110547323A (en) * 2019-06-28 2019-12-10 广东省农业科学院蚕业与农产品加工研究所 Formula for protecting eyes of frozen fish and using method thereof
CN110800797A (en) * 2019-11-27 2020-02-18 浙江省海洋水产研究所 Micro-freezing preservation method for swimming crabs
CN110800797B (en) * 2019-11-27 2023-05-09 浙江省海洋水产研究所 Swimming crab micro-freezing fresh-keeping method
CN111000168A (en) * 2019-12-19 2020-04-14 武汉良之隆食材股份有限公司 Processing method of ice mountain mussel capable of being used as thorn body
CN113229353A (en) * 2021-06-02 2021-08-10 渤海大学 Preparation and thawing method of frozen pear dices thawing solution
CN114431283A (en) * 2022-02-22 2022-05-06 集美大学 Fish composite antifreeze agent and application thereof

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Application publication date: 20140212