CN102396717A - Pleurotus stem dietary fiber prepared with actinomucor elegans fermentation method and application thereof - Google Patents
Pleurotus stem dietary fiber prepared with actinomucor elegans fermentation method and application thereof Download PDFInfo
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- CN102396717A CN102396717A CN2011103543434A CN201110354343A CN102396717A CN 102396717 A CN102396717 A CN 102396717A CN 2011103543434 A CN2011103543434 A CN 2011103543434A CN 201110354343 A CN201110354343 A CN 201110354343A CN 102396717 A CN102396717 A CN 102396717A
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Abstract
The invention discloses pleurotus stem dietary fiber prepared with an actinomucor elegans fermentation method. The method comprises the following preparation steps of: A, culturing an actinomucor elegans strain; B, fermenting actinomucor elegans to prepare pleurotus stem dietary fiber: adding 10 percent by volume of the strain obtained in the step A into a mixed liquid of pleurotus stem powder and water, culturing in ventilated state, and fermenting at the temperature 26-30 DEG C to obtain a zymotic fluid; and C, performing after-treatment on the zymotic fluid, wherein the after-treatment mainly comprises a homogenizing step for homogenizing the zymotic fluid under the pressure 120 MPa. The invention further provides a method for preparing a nutritional food or beverage from the obtained pleurotus stem dietary fiber. According to the invention, efficient recycling of pleurotus stems is realized.
Description
Technical field
The present invention relates to the comprehensive utilization technique field of flat mushroom handle.
Background technology
Flat mushroom is that whole world cultivated area is maximum, the production cultivation technique is the most ripe, biology efficient is high, a kind of edible mushroom of better economic result, also is one of four kinds of maximum main edible mushrooms of the present cultivated area of China.Edible mushroom all has critical role in people's daily life and economic life, mushroom industry has become a pillar industry in the agricultural.In the export processing commercialization process of flat mushroom, must cut off the mushroom handle, only this China will produce millions of tons flat mushroom handle every year, and this part resource is very huge.Flat mushroom handle and fructification are to be differentiated to form by mycelium equally; Be rich in effective bioactive ingredients; Flat mushroom handle fibrosis is high; Mainly be to become to be grouped into by the somatic cells wall, the formed health care of its fiber quality and bioactivator be worth be string incomparable, be a kind of source of fabulous senior natural dietary fiber.
It is the general name of the carbohydrate of one type of non-usability being polymerized of residue that dietary fiber is meant with monose such as glucose, can be divided into soluble dietary fiber (SDF) and insolubility dietary fiber (IDF), and both physiological functions in human body are different.The insolubility dietary fiber mainly comprises cellulose, hemicellulose and lignin etc., can absorb moisture, stimulates the wriggling of intestines, quickens defecation, reduces the probability of suffering from intestinal cancer; Water-soluble dietary fiber mainly comprises stored substance and secretion, microbial polysaccharide and the synthetic polysaccharide of plant cell, has the function of adjustment carbohydrate and lipid metabolism, and reduction human cholesterol content, angiocardiopathy preventing are had good result.See that from the modern nutriology angle food fiber has become indispensable important function composition in the human foods.People need take the photograph the cellulose of people's some every day from meals, this can not only promote gastrointestinal motility, also can prevent multiple diseases such as colon cancer.Utilize to contain the high characteristics of dietary fiber in the flat mushroom handle, can develop the fiber healthy food.With the flat mushroom handle clean, oven dry, pulverize, through the fermented extracted dietary fiber, process the fiber powder and be added in bread, cake and the drink, become one of its main component.Worldwide owing to the development of healthy food, people increase the interest of dietary fiber day by day at present.
In the last few years, " ciril disease " that cause owing to meals imbalance or overnutrition occurred in China, and obesity, hypertension, coronary heart disease, diabetes and colon cancer etc. have become the principal disease of harm China people ' s health.Therefore, under this background, the trophic function of dietary fiber and research and development thereof become a crucial problem of China.Contain a large amount of dietary fibers in the flat mushroom handle, it is carried out the defective that processing and utilization can improve dietary fiber insufficiency of intake in people's eating habit.
At present, the preparation method of dietary fiber mainly contains following several kinds: chemical method, enzyme process, membrane separation process, fermentation method etc.Chemical method be meant with raw material through dry, grind the back and adopt chemical reagent to extract and the method that prepare dietary fiber, thereby need a large amount of washings after the reaction end cause the loss of water-soluble dietary fiber.Enzyme process is to remove the component of other except that dietary fiber in the raw material one by one with plurality of enzymes, obtains the method for dietary fiber at last, and this method wants high than the chemical method cost, operates also more loaded down with trivial details.It is less to adopt membrane separation process to prepare the report of dietary fiber, and this method is the dietary fiber for preparing different molecular weight through the molecule interception that changes film, and shortcoming is to prepare insoluble diedairy fiber, and this method is higher to equipment requirements.
Summary of the invention
The technical problem that the present invention will solve provides the flat mushroom handle dietary fiber of a kind of graceful radiation Mucor fermentation method preparation; Realization is to the high efficiente callback utilization of flat mushroom handle; Extraction nutritional labeling wherein is dietary fiber especially, and the dietary fiber that makes is used to prepare nutraceutical and drink.
For solving the problems of the technologies described above, the technical scheme that the present invention taked is following.
The flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method preparation prepares according to following steps:
A, graceful radiation mucor strain are cultivated, and get bacteria culture fluid;
B, utilize graceful radiation Mucor fermenting process of preparing flat mushroom handle dietary fiber: in the mixed liquor of flat mushroom handle powder and water, add the steps A gained bacteria culture fluid of 10% volume fraction, ventilation is cultivated, and fermentation temperature is controlled at (26-30) ℃, gets zymotic fluid;
The post-processed of C, zymotic fluid: post-processed comprises homogenizing step, makes zymotic fluid under 120MPa pressure, carry out homogeneous.
As a kind of optimal technical scheme of the present invention, in the steps A, consisting of of bacterium culture medium by weight: flat mushroom handle powder (2.8-3.2) %, quick-acting carbon sources (1.8-2.2) %, peptone (0.9-1.1) %, mineral matter (0.2-0.4) %, water surplus; The condition of culture that bacterial classification is cultivated is: ventilation is cultivated, fermentation temperature (26-30) ℃, cultivation cycle 24h, bacteria culture fluid.
As a kind of optimal technical scheme of the present invention, the flat mushroom handle powder that said flat mushroom handle powder was 80 mesh sieves; Said quick-acting carbon source is a glucose; Said mineral matter comprises 0.2% KH
2PO
4With 0.1% MgSO
4.7H
2O.
As a kind of optimal technical scheme of the present invention, the concrete operation method of step B is:
The primary election of B-1, flat mushroom handle: remove the foreign material in the flat mushroom handle;
The cleaning of B-2, flat mushroom handle: the circulating water of flat mushroom handle with 15-25 ℃ cleaned up;
The drying of B-3, flat mushroom handle: 50 ℃ of dryings 5 hours;
The pulverizing of B-4, flat mushroom handle: with organizing pulverizer to pulverize and cross 80 mesh sieves, extracting screen underflow gets flat mushroom handle powder;
B-6, charging, sterilization, fermentation medium: with flat mushroom handle powder and water by weight 1: (7-9) triangular flask of packing into places 95 ℃ of water-baths to heat 10min;
B-7, bacterial classification inoculation: on go on foot the gained fermentation medium and in desinfection chamber, be cooled to room temperature, inoculation step A gained bacteria culture fluid; Inoculum concentration is 10% of a fermentation medium volume;
B-8, fermented and cultured: rotary shaking speed 200r/min, ventilation is cultivated, 28 ℃ of temperature, cultivation cycle 24h gets zymotic fluid.
As a kind of optimal technical scheme of the present invention, the concrete operation method of step C is:
C-1, zymotic fluid homogeneous: step B gained zymotic fluid carries out homogeneous 10min under 120MPa pressure;
C-2, Protease Treatment: step C-1 gained zymotic fluid is handled through neutral proteinase hydrolysis; Wherein, the consumption of neutral proteinase is (3-5) % by weight, handles duration 1-2h, and treatment temperature is 45-55 ℃, and pH is 6-8, during through adding the pH value that buffer keeps feed liquid;
C-3, suction filtration: step C-2 gained feed liquid suction filtration under vavuum pump, must filtrate and filter residue; Filter residue is the insolubility dietary fiber, and soluble dietary fiber is present in the middle of the filtrating;
C-4, alcohol are analysed: on go on foot gained filtrating and be concentrated into (20-25) % of original volume through rotary evaporator, add 95% ethanol then, the volume ratio of concentrated filtrate and ethanol is 4: 1, leaves standstill 5-7h, filters to obtain precipitating, alcohol is recycled; Gained is precipitated as soluble dietary fiber;
The deposition of the filter residue of C-5, drying, pulverizing: step C-3 and step C-4 is dry 3h under 50 ℃ of temperature respectively; Be ground into powder respectively, promptly get solubility flat mushroom handle dietary fiber finished product and insolubility flat mushroom handle dietary fiber finished product.
Above-mentioned flat mushroom handle dietary fiber is used to prepare the purposes of nutraceutical.
Utilize above-mentioned flat mushroom handle dietary fiber to prepare the method for bread, its step comprises:
A and face: with flat mushroom handle dietary fiber and flour, yeast, the abundant mixing of milk powder, wherein the interpolation weight ratio of flat mushroom handle dietary fiber is 3-9%, adds that water is stirred to that dough soft or hard appropriateness, elasticity are good, toughness and extensibility is moderate and dry tack free is bright and clean;
B, stripping and slicing: the dough that will become reconciled is cut into (50-150) gram/piece face piece;
C, rub circle with the hands: will cut apart good face piece and be twisted into uniform spherical, and make because cutting and ruined gluten network structure is able to repair, and discharge carbon dioxide, and make yeast be able to breeding and fermentation;
D, fermentation: will rubbing dough behind the circle with the hands, to place temperature be that 38-40 ℃, relative humidity are the fermenting case of the 80%-85% 60-90min that ferments, and 1.5-2.0 bright and clean with dough surface, that expand into original volume doubly is as the criterion;
E, cure: the dough that ferments is sent into baking oven, and initial temperature is for getting angry the fiery 250-260 of 170 ℃, times ℃, behind the 3-4min; Dough surface is colouring slightly, and the temperature of will getting angry again transfers to the fiery temperature of 220 ℃, times and transfers to 200 ℃, keeps 5-6min; Come out of the stove, and rapidly at bread surface brush one deck stand oil;
F, cooling get finished product.
Utilize above-mentioned flat mushroom handle dietary fiber to prepare the method for cake, its step comprises:
A, making beating: egg, white sugar, water and flat mushroom handle dietary fiber are added mixer, dismiss 3-7min at a high speed and makes its volume increase to original 2.5-3.5 doubly, the egg liquid integral body fluffy foam-like that is creamy white; Wherein, the addition of calculating by weight flat mushroom handle dietary fiber is 3-7%;
B, mixing batter: the flour that sieves is evenly added in the egg liquid of having dismissed in the mixer, stir 1min, mix to such an extent that cake is stuck with paste; Get the part cake and stick with paste mensuration proportion, the residue cake is stuck with paste into mould;
C, go into mould: be poured into the roasting mould of roundlet behind the mixing batter, water modulus and be 2/3 of roasting model volume; Earlier evenly coat vegetable oil before the moulding at roasting mould inwall;
D, baking: toast immediately behind the moulding, Control for Kiln Temperature is 160-200 ℃, baking 20min, and finished product is cooled to room temperature.
Utilize soluble dietary fiber in the above-mentioned flat mushroom handle dietary fiber to prepare the method for drink, its step comprises:
The screening of A, hawthorn, cleaning: choose maturation, full, fresh haw berry,, stoning clean with flushing with clean water, broken through the fruit bruisher;
B, softening, lixiviate: adopt 2 times extraction, the 1st time extraction time is 20-30min, and temperature is 90-95 ℃, and amount of water is 2 times of haw berry weight, gets leaching liquor after softening; Pomace adds the water of 2 times of raw material weights again, lixiviate 5-10h, and temperature is 40-45 ℃, merges the leaching liquor of twice gained;
C, coarse filtration: the frequent press filtration of leaching liquor obtains the hawthorn Normal juice of clarification;
D, clarification: in hawthorn Normal juice, add an amount of pectase and carry out clarifying treatment.Hawthorn Normal juice is heated to 85 ℃ of sterilization 15min, and is to be cooled to 40 ℃, adds people's 0.06% pectase and stir, and leaves standstill 2-4h, can obtain good clarifying effect;
E, mixing preparation: added the 4-8% soluble dietary fiber again with in 90 ℃ of water of haw juice adding in 1: 5 by volume, 0.2% stabilizing agent and 12% white granulated sugar, and constantly stir, make it to dissolve fully.Stabilizer element is sodium carboxymethylcellulose and xanthans, and mass ratio is 1: 1;
F, homogeneous: the feed liquid that mixes is carried out homogeneous, and pressure is 40MPa, and temperature is 60-70 ℃, and the time is 2min;
G, the degassing: the beverage behind the homogeneous is to outgas under the 90.7-93.3KPa condition in vacuum immediately, discharges the air in the beverage;
H, sterilization, can, envelope bottle, cooling: the beverage after the degassing sterilize immediately (20min/85 ℃) irritate then that the people fully cleans and the vial with moise-heat sterilization in; Seal with the lid behind the steam sterilizing rapidly; Be cooled to room temperature, be finished product through after the assay was approved.
Adopt the beneficial effect that technique scheme produced to be:
1. the AOAC985.29 method that adopts standard is (referring to document: WILLIAM H.AOAC official method 985.29; Total dietary fiber in food and food products.Official methods of analysis of AOAC INTERNATIONAL [S] .17th ed.2000; 2 (45): 78-80.) measure prepared dietary fiber, measure the result and see the following form.
Table 1. productive rate contrast experiment table
The productive rate that flat mushroom handle powder is carried total dietary fiber through general water is 19.06%, and soluble dietary fiber is merely 2.12%; The productive rate of total dietary fiber is 32.58% after the fermentation of graceful radiation Mucor, and the productive rate of insolubility dietary fiber is 26.96%, and the productive rate of soluble dietary fiber is 5.62%; After passing through super-high-pressure homogenization again, the productive rate of dietary fiber brings up to 36.17%, and the productive rate of insolubility dietary fiber is 26.01%, and the productive rate of soluble dietary fiber has reached 10.16%.
Graceful radiation mucor filamentous fungi of the present invention, the long speed of fermented and cultured is fast, capacity of decomposition is strong, and itself contains dietary fiber components such as glucan thalline; Compare with chemical method, enzyme process, membrane separation process and extract dietary fiber; It is the enzyme reaction through self producing during the fermentation; Can be with the glycosidic bond fracture of the cellulose in the flat mushroom handle, hemicellulose components; Produce new reducing end under neutral, the big molecule aggregation degree of dietary fiber is constantly descended, being converted into microbial polysaccharide, thalline cellulose etc. is the activated dietary fiber of having more of master.Combine instantaneous superhigh pressure technique again, make bigger insolubility dietary fiber strand break to form the little molecule of solubility; It is loose that the structure of other insoluble diedairy fibers also becomes, and it is utilized in human body more easily, further obtains the high-quality dietary fiber.
2. the bread quality of the present invention's preparation is evaluated:, adopt the comprehensive grading method according to bread flavor evaluation convention; The quality scoring of bread will be taken all factors into consideration the outward appearance (color of the leather, cortex, profile etc.) and the endoplasm each side factors such as (flesh look, sense of touch, mouthfeel, taste smells) of bread, provides correct evaluation.
But an amount of dietary fiber instead of part starch is to reduce the calorific value of product; Dietary fiber also has good water holding and holds the oiliness ability, can improve the water content of finished product bread, makes the bulk softness of bread, and the freshness date that has prolonged bread is improved its performance, and makes product have the physiologically active of dietary fiber; Therefore bread its specific structure characteristics are to be formed by the desmachyme that gluten property protein forms, and in fiber bread, need to such an extent that guarantee a certain amount of wheat gluten; The consumption of dietary fiber will suit; Confirmed the dietary fiber addition of 3-9% through test of many times,, be difficult to form the peculiar structure of bread at most if the dietary fiber addition is crossed with 6% for the most suitable; And mouthfeel, color and luster also can receive tangible influence, but the consumption of dietary fiber is crossed the purpose that does not reach reduction product calorific value at least.
3. add content and be 3-7% especially 5% flat mushroom handle dietary fiber that cake is stuck with paste is bulk, fine and smooth, form spongy cake when beating eggs easily and stick with paste; Contain higher level sweet taste batching in the cake, also contain simultaneously than high-moisture, therefore when baking, can condense into soft product, the adsorbable large quantity of moisture of dietary fiber helps product and solidifies with fresh-keeping, can also reduce the product energy simultaneously; Add an amount of dietary fiber and help to increase small product size, its reason is that dietary fiber strengthens bubble stability in the dough; The dietary fiber gluten that can weaken is beneficial to and bakes process cake base and expand and send out, and has improved dietary fiber content in the cake simultaneously, has increased its nutrition and health care function.
4. the kind of dietary fiber is remarkable to the influence of fiber drink stability; If water insoluble fibre system fibre drink, then suspension is more, and layering, deposited phenomenon take place easily, and product stability is relatively poor; On the contrary, water-soluble dietary fiber can be in the same place with water-soluble separating, and is fewer with the beverage suspension that this kind fiber makes, be not easy to take place layering, deposited phenomenon, stable relatively good; Fibre drink is a kind of Unstable Systems, needs to add an amount of stabilizing agent in the preparation process; Confirmed the soluble dietary fiber addition of 4-8% through test, with 6% for the most suitable, the fibre drink consistency ratio that the fiber addition more than 6% makes is higher, considers the mouthfeel of beverage, and the fiber addition should not be too high, should be controlled at below 6%.
The present invention has made full use of the flat mushroom processing byproduct---the flat mushroom handle, and the dietary fiber that extracts is added into nutraceutical and drink, improved its nutrition and health care function.
The specific embodiment
Following examples have specified the present invention.Various raw material used in the present invention and items of equipment are conventional commercially available prod, all can buy directly through market to obtain.The used bacterial classification of the present invention is: graceful radiation Mucor AS3.2778 bacterial strain, provide by Beijing king food group that makes peace, and also can buy from DSMZ; Its preservation condition is: 4 ℃ of refrigerator preservations, and tube was 1 time in 6 months, and activation is spent the night under the preceding room temperature of use.
Embodiment 1
The flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method preparation prepares according to following steps:
Can earlier grace be radiated Mucor cultivates on the PDA slant medium in advance.The weight of PDA slant medium consists of: glucose 2.0%, potato 15.0%, wheat bran 5.0%, agar 2.0%, water surplus.Its preparation method is: potato slice and wheat bran are boiled 20min altogether, and four layers of filtered through gauze discard filter residue, and glucose, agar incorporate filtrating, add water, the pH nature, and 0.1MPa, 121 ℃ of sterilization 30min promptly get.Can earlier grace be radiated Mucor cultivates a period of time on this PDA slant medium in advance.
A, graceful radiation mucor strain are cultivated: consisting of of bacterium culture medium by weight: cross the flat mushroom handle powder 3.0% of 80 mesh sieves, glucose 2.0%, peptone 1.0%, KH
2PO
40.2%, MgSO
4.7H
2O 0.1%, water surplus; The pH nature, 0.1MPa, 121 ℃ of sterilization 30min; Mycelium on the PDA slant medium is seeded in this bacterium culture medium, and 28 ℃ of temperature conditions are gone up the shaking table ventilation down and are cultivated 24h, and biomass reaches more than the 35g/mL (fresh weight), gets bacteria culture fluid, uses bacterial classification as fermentation;
B, the graceful radiation of utilization Mucor fermenting process of preparing flat mushroom handle dietary fiber, concrete operation method is:
The primary election of B-1, flat mushroom handle: remove the foreign material in the flat mushroom handle;
The cleaning of B-2, flat mushroom handle: the circulating water of flat mushroom handle with 20 ℃ cleaned up;
The drying of B-3, flat mushroom handle: 50 ℃ of dryings 5 hours;
The pulverizing of B-4, flat mushroom handle: with organizing pulverizer to pulverize and cross 80 mesh sieves, extracting screen underflow gets flat mushroom handle powder;
B-5, charging, sterilization, fermentation medium: with flat mushroom handle powder and the water triangular flask of packing into by weight 1: 8, place 95 ℃ of water-baths to heat 10min;
B-6, bacterial classification inoculation: on go on foot the gained fermentation medium and in desinfection chamber, be cooled to room temperature, inoculation step A gained bacteria culture fluid; Inoculum concentration is 10% of a fermentation medium volume;
B-7, fermented and cultured: shake bottle and be 500ml, interior dress 200ml nutrient solution, rotary shaking speed 200r/min, ventilation is cultivated, 28 ℃ of temperature, cultivation cycle 24h gets zymotic fluid;
The post-processed of C, zymotic fluid, concrete operation method is:
C-1, zymotic fluid homogeneous: step B gained zymotic fluid carries out homogeneous 10min under 120MPa pressure;
C-2, Protease Treatment: step C-1 gained zymotic fluid is handled through neutral proteinase hydrolysis; Wherein, the consumption of neutral proteinase is by weight 4%, handles duration 1.5h, and treatment temperature is 50 ℃, and pH is 7, during through adding the pH value that buffer keeps feed liquid;
C-3, suction filtration: step C-2 gained feed liquid suction filtration under vavuum pump, must filtrate and filter residue; Filter residue is the insolubility dietary fiber, and soluble dietary fiber is present in the middle of the filtrating;
C-4, alcohol are analysed: on go on foot gained filtrating and be concentrated into (20-25) % of original volume through rotary evaporator, add 95% ethanol then, the volume ratio of concentrated filtrate and ethanol is 4: 1, leaves standstill 5-7h, filters to obtain precipitating, alcohol is recycled; Gained is precipitated as soluble dietary fiber;
The deposition of the filter residue of C-5, drying, pulverizing: step C-3 and step C-4 is dry 3h under 50 ℃ of temperature respectively; Be ground into powder respectively, promptly get solubility flat mushroom handle dietary fiber finished product and insolubility flat mushroom handle dietary fiber finished product; The two can use respectively, also can mix use.
Embodiment 2
Utilize above-mentioned flat mushroom handle dietary fiber to prepare the method for bread, its step comprises:
A and face: with flat mushroom handle dietary fiber and flour, yeast, the abundant mixing of milk powder, wherein the interpolation weight ratio of flat mushroom handle dietary fiber is 3-9%, adds that water is stirred to that dough soft or hard appropriateness, elasticity are good, toughness and extensibility is moderate and dry tack free is bright and clean;
B, stripping and slicing: the dough that will become reconciled is cut into (50-150) gram/piece face piece;
C, rub circle with the hands: will cut apart good face piece and be twisted into uniform spherical, and make because cutting and ruined gluten network structure is able to repair, and discharge carbon dioxide, and make yeast be able to breeding and fermentation, and simultaneously, various batchings will be more evenly distributed;
D, fermentation: will rubbing dough behind the circle with the hands, to place temperature be that 38-40 ℃, relative humidity are the fermenting case of the 80%-85% 60-90min that ferments, and 1.5-2.0 bright and clean with dough surface, that expand into original volume doubly is as the criterion;
E, cure: the dough that ferments is sent into baking oven, and initial temperature is for getting angry the fiery 250-260 of 170 ℃, times ℃, behind the 3-4min; Dough surface is colouring slightly, and the temperature of will getting angry again transfers to the fiery temperature of 220 ℃, times and transfers to 200 ℃, keeps 5-6min; Come out of the stove; And rapidly at bread surface brush one deck stand oil, the dehydration drying shrinkage also can play the effect that beautifies product simultaneously when preventing that bread from storing;
F, cooling, packing, storage: when bread is naturally cooled to 35 ℃ under the condition of relative humidity 75%, promptly available transparent plastic food bag packing through sterilization processing, and at room temperature store.
Embodiment 3
Utilize above-mentioned flat mushroom handle dietary fiber to prepare the method for cake, its step comprises:
A, making beating: egg, white sugar, water and flat mushroom handle dietary fiber are added mixer, dismiss 3-7min at a high speed and makes its volume increase to original 2.5-3.5 doubly, the egg liquid integral body fluffy foam-like that is creamy white; Wherein, the addition of calculating by weight flat mushroom handle dietary fiber is 3-7%;
B, mixing batter: the flour that sieves is evenly added in the egg liquid of having dismissed in the mixer, stir 1min, mix to such an extent that cake is stuck with paste; Get the part cake and stick with paste mensuration proportion, the residue cake is stuck with paste into mould;
C, go into mould: be poured into the roasting mould of roundlet behind the mixing batter, water modulus and be 2/3 of roasting model volume; Earlier evenly coat vegetable oil before the moulding at roasting mould inwall;
D, baking: toast immediately behind the moulding, Control for Kiln Temperature is 160-200 ℃, baking 20min, and finished product is cooled to room temperature.
Embodiment 4
Utilize soluble dietary fiber in the above-mentioned flat mushroom handle dietary fiber to prepare the method for drink, its step comprises:
The screening of A, hawthorn, cleaning: choose maturation, full, fresh haw berry,, stoning clean with flushing with clean water, broken through the fruit bruisher;
B, softening, lixiviate: adopt 2 times extraction, the 1st time extraction time is 20-30min, and temperature is 90-95 ℃, and amount of water is 2 times of haw berry weight, gets leaching liquor after softening; Pomace adds the water of 2 times of raw material weights again, lixiviate 5-10h, and temperature is 40-45 ℃, merges the leaching liquor of twice gained;
C, coarse filtration: the frequent press filtration of leaching liquor obtains the hawthorn Normal juice of clarification;
D, clarification: in hawthorn Normal juice, add an amount of pectase and carry out clarifying treatment.Hawthorn Normal juice is heated to 85 ℃ of sterilization 15min, and is to be cooled to 40 ℃, adds people's 0.06% pectase and stir, and leaves standstill 2-4h, can obtain good clarifying effect;
E, mixing preparation: added the 4-8% soluble dietary fiber again with in 90 ℃ of water of haw juice adding in 1: 5 by volume, 0.2% stabilizing agent and 12% white granulated sugar, and constantly stir, make it to dissolve fully.Stabilizer element is sodium carboxymethylcellulose and xanthans, and mass ratio is 1: 1;
F, homogeneous: the feed liquid that mixes is carried out homogeneous, and pressure is 40MPa, and temperature is 60-70 ℃, and the time is 2min;
G, the degassing: the beverage behind the homogeneous is to outgas under the 90.7-93.3KPa condition in vacuum immediately, discharges the air in the beverage;
H, sterilization, can, envelope bottle, cooling: the beverage after the degassing sterilize immediately (20min/85 ℃) irritate then that the people fully cleans and the vial with moise-heat sterilization in; Seal with the lid behind the steam sterilizing rapidly; Be cooled to room temperature, be finished product through after the assay was approved.
Foregoing description only proposes as the enforceable technical scheme of the present invention, not as the single restrictive condition to its technical scheme itself.
Claims (9)
1. the flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method preparation is characterized in that preparing according to following steps:
A, graceful radiation mucor strain are cultivated, and get bacteria culture fluid;
B, utilize graceful radiation Mucor fermenting process of preparing flat mushroom handle dietary fiber: in the mixed liquor of flat mushroom handle powder and water, add the steps A gained bacteria culture fluid of 10% volume fraction, ventilation is cultivated, and fermentation temperature is controlled at (26-30) ℃, gets zymotic fluid;
The post-processed of C, zymotic fluid: post-processed comprises homogenizing step, makes zymotic fluid under 120MPa pressure, carry out homogeneous.
2. the flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method according to claim 1 preparation; It is characterized in that: in the steps A; Consisting of of bacterium culture medium by weight: flat mushroom handle powder (2.8-3.2) %, quick-acting carbon sources (1.8-2.2) %, peptone (0.9-1.1) %; Mineral matter (0.2-0.4) %, water surplus; The condition of culture that bacterial classification is cultivated is: ventilation is cultivated, fermentation temperature (26-30) ℃, cultivation cycle 24h, bacteria culture fluid.
3. the flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method according to claim 2 preparation is characterized in that: the flat mushroom handle powder that said flat mushroom handle powder was 80 mesh sieves; Said quick-acting carbon source is a glucose; Said mineral matter comprises 0.2% KH
2PO
4With 0.1% MgSO
4.7H
2O.
4. the flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method according to claim 1 preparation, it is characterized in that: the concrete operation method of step B is:
The primary election of B-1, flat mushroom handle: remove the foreign material in the flat mushroom handle;
The cleaning of B-2, flat mushroom handle: the circulating water of flat mushroom handle with 15-25 ℃ cleaned up;
The drying of B-3, flat mushroom handle: 50 ℃ of dryings 5 hours;
The pulverizing of B-4, flat mushroom handle: with organizing pulverizer to pulverize and cross 80 mesh sieves, extracting screen underflow gets flat mushroom handle powder;
B-5, charging, sterilization, fermentation medium: with flat mushroom handle powder and water by weight 1: (7-9) triangular flask of packing into places 95 ℃ of water-baths to heat 10min;
B-6, bacterial classification inoculation: on go on foot the gained fermentation medium and in desinfection chamber, be cooled to room temperature, inoculation step A gained bacteria culture fluid; Inoculum concentration is 10% of a fermentation medium volume;
B-7, fermented and cultured: rotary shaking speed 200r/min, ventilation is cultivated, 28 ℃ of temperature, cultivation cycle 24h gets zymotic fluid.
5. the flat mushroom handle dietary fiber of graceful radiation Mucor fermentation method according to claim 1 preparation, it is characterized in that: the concrete operation method of step C is:
C-1, zymotic fluid homogeneous: step B gained zymotic fluid carries out homogeneous 10min under 120MPa pressure;
C-2, Protease Treatment: step C-1 gained zymotic fluid is handled through neutral proteinase hydrolysis; Wherein, the consumption of neutral proteinase is (3-5) % by weight, handles duration 1-2h, and treatment temperature is 45-55 ℃, and pH is 6-8, during through adding the pH value that buffer keeps feed liquid;
C-3, suction filtration: step C-2 gained feed liquid suction filtration under vavuum pump, must filtrate and filter residue; Filter residue is the insolubility dietary fiber, and soluble dietary fiber is present in the middle of the filtrating;
C-4, alcohol are analysed: on go on foot gained filtrating and be concentrated into (20-25) % of original volume through rotary evaporator, add 95% ethanol then, the volume ratio of concentrated filtrate and ethanol is 4: 1, leaves standstill 5-7h, filters to obtain precipitating, alcohol is recycled; Gained is precipitated as soluble dietary fiber;
The deposition of the filter residue of C-5, drying, pulverizing: step C-3 and step C-4 is dry 3h under 50 ℃ of temperature respectively; Be ground into powder respectively, promptly get solubility flat mushroom handle dietary fiber finished product and insolubility flat mushroom handle dietary fiber finished product.
6. the described flat mushroom handle of claim 1 dietary fiber is used to prepare the purposes of nutraceutical.
7. according to the said purposes of claim 6, its characterization step comprises: said flat mushroom handle dietary fiber is used to prepare bread.
8. according to the said purposes of claim 6, its characterization step comprises: said flat mushroom handle dietary fiber is used to prepare cake.
9. according to the said purposes of claim 6, its characterization step comprises: the solubility flat mushroom handle dietary fiber in the said flat mushroom handle dietary fiber is used to prepare drink separately.
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| CN107319515A (en) * | 2017-06-15 | 2017-11-07 | 西南大学 | A kind of preparation method of asparagus dietary fiber |
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Effective date of registration: 20161207 Address after: 071002 Baoding cooperation Road, Hebei, No. 187 Patentee after: Wang You Address before: 071002 Baoding cooperation Road, Hebei, No. 187 Patentee before: Baoding Chunhua Biotechnology Co.,Ltd. |