CN102382202A - Method for extracting water-soluble polysaccharide from morinda citrifolia fruits - Google Patents
Method for extracting water-soluble polysaccharide from morinda citrifolia fruits Download PDFInfo
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- CN102382202A CN102382202A CN2011102648320A CN201110264832A CN102382202A CN 102382202 A CN102382202 A CN 102382202A CN 2011102648320 A CN2011102648320 A CN 2011102648320A CN 201110264832 A CN201110264832 A CN 201110264832A CN 102382202 A CN102382202 A CN 102382202A
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- morinda citifolia
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- morinda citrifolia
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a method for extracting a water-soluble polysaccharide from morinda citrifolia fruits. The method comprises the following steps of 1, a drying and crushing step comprising cleaning up fresh morinda citrifolia fruits, drying in an air, drying in an oven, and crushing by a plant crushing device, 2, a supercritical CO2 fluid extraction lipid-removal step comprising putting the morinda citrifolia fruit powder obtained by the step 1 into an extractor, feeding CO2 into the extractor, and carrying out supercritical extraction to remove the most of lipid, 3, an extraction step comprising mixing distilled water and the residues obtained by the supercritical CO2 fluid extraction lipid-removal step, carrying out extraction in a hot bath, cooling, and filtering to obtain filtrate, and 4, an alcohol precipitation step comprising centrifuging the filtrate to obtain clarified liquor, mixing the clarified liquor and 85% of alcohol, stirring, and carrying out centrifugation to obtain a morinda citrifolia fruit polysaccharide. The method has the advantages of simple processes, high extraction ratio, and no denaturation and pollution produced in raw material extraction.
Description
Technical field
The present invention relates to the process for extracting of water-soluble polysaccharide, relate in particular to the method for from the Morinda Citifolia fruit, extracting water-soluble polysaccharide.
Background technology
Morinda Citifolia is the Rubiaceae platymiscium, the evergreen perennial broad-leaved shrub in the torrid zone.Its fruit, leaf, limb, root all can be used as medicine.It really is white base of leaf fruit, is rich in multiple nutritional components, and a generation have the laudatory title of " celestial fruit " in the South Pacific.
Pharmacological evaluation proves in the Morinda Citifolia fruit a kind of anti-tumor activity mucopolysaccharide is arranged.Polysaccharide has complicated biological activity and function, is one of functional ingredient main in the Morinda Citifolia fruit.Researchs such as bang green grass or young crops show, the mixed polysaccharide that the polysaccharide in the Morinda Citifolia fruit is made up of monose such as Fucose, wood sugar, seminose, semi-lactosi and fructose, and average content (with glucose meter) is about 11.77%.Research shows that Morinda Citifolia fruit juice polysaccharide has antitumour activity, can suppress growth of tumor through regulating host immune system, and the ability of the mouse peritoneal emigrated cell kill cancer cell behind the beautiful polysaccharide of injection promise has increased by 9 times.The beautiful polysaccharide of promise can produce cytokines such as nitrogen protoxide, interleukin-22 1, tumour necrosis factor, interleukin-22 12 by the inducing mouse peritoneal macrophage.The beautiful polysaccharide of promise also can suppress lymphocyte and produce interleukin-22 4.Researchs such as bang green grass or young crops show that the Morinda Citifolia fruit water-soluble polysaccharide that produce in Hainan has the very strong removing superoxide radical and the effect of hydroxy radical qiao, has certain activity of fighting against senium.
Still be in the starting stage for STUDY ON POLYSACHAROSE in the Morinda Citifolia fruit, do not see the patent report of associated extraction technology.
Summary of the invention
The present invention aims to provide the method for from the Morinda Citifolia fruit, extracting water-soluble polysaccharide.
To achieve these goals, technical scheme of the present invention is following:
(1) drying and crushing: fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to the 40-60 order with the plant kibbler under 40-70 ℃ of temperature;
(2) supercritical CO
2Fluid extraction degreasing: above-mentioned Morinda Citifolia fruit powder is placed extractor, feed CO
2, carry out supercritical extraction, remove most of lipid material;
(3) lixiviate: the residue and the zero(ppm) water that will extract after the degreasing are that 1:15-20 mixes by solid-to-liquid ratio, lixiviate 30-120min in 80-100 ℃ of hot water bath, and cooled and filtered obtains filtrating;
(4) alcohol precipitation: it is centrifugal to filtrate, and obtains clear liquor, vacuum concentration, and liquid concentrator mixes, stirs with 85% alcohol, centrifugal 20-30min, the deposition drying obtains Morinda Citifolia fruit polysaccharide.
The supercritical extraction temperature is 35-60 ℃ in the said step (2), and extracting pressure is 28-45MPa, and the time is 40-70min, CO
2Flow is 20L/h-35L/h.
Centrifugal rotational speed is 2800-3100rpm in the said step (4).
Anacidity, alkali, enzyme and organic solvent are to the influence of Morinda Citifolia fruit polysaccharide in the leaching process of the present invention, and gained Morinda Citifolia fruit polysaccharide biological activity is higher.Simultaneously, the present invention does not also have processing safety and environmental problem.
The product that the present invention obtains promptly can be used as foodstuff additive, and it is directly edible also to can be used as protective foods or medicine, for instant edible can be a base-material with Morinda Citifolia fruit polysaccharide of the present invention, adds that pure water is wetting, granulation, compressing tablet, processes tablet.
To combine embodiment to further specify the present invention below, but the scope that the present invention requires to protect is not limited to the following example.
Embodiment
Embodiment 1:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 50 orders with the plant kibbler under 55 ℃ of temperature, take by weighing 1kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 60 ℃, and extracting pressure is 30MPa, and the time is 70min, CO
2Flow is 20L/h, removes most of lipid material, is that 1:18 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 30min in 80 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 2800rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 25min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 12.7g.
Embodiment 2:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 60 orders with the plant kibbler under 60 ℃ of temperature, take by weighing 2kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 45 ℃, and extracting pressure is 30MPa, and the time is 50min, CO
2Flow is 35L/h, removes most of lipid material, is that 1:15 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 120min in 80 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 3100rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 20min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 30.1g.
Embodiment 3:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 40 orders with the plant kibbler under 70 ℃ of temperature, take by weighing 6kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 50 ℃, and extracting pressure is 40MPa, and the time is 60min, CO
2Flow is 28L/h, removes most of lipid material, is that 1:16 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 100min in 100 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 3100rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 30min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 93.5g.
Embodiment 4:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 60 orders with the plant kibbler under 70 ℃ of temperature, take by weighing 6kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 35 ℃, and extracting pressure is 28MPa, and the time is 40min, CO
2Flow is 35L/h, removes most of lipid material, is that 1:18 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 30min in 100 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 3100rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 20min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 72.1g.
Embodiment 5:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 60 orders with the plant kibbler under 50 ℃ of temperature, take by weighing 10kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 60 ℃, and extracting pressure is 45MPa, and the time is 40min, CO
2Flow is 20L/h, removes most of lipid material, is that 1:20 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 120min in 80 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 2800rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 30min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 109.9g.
Embodiment 6:
Fresh Morinda Citifolia really cleaned up dry, put in the baking oven that the oven dry back is crushed to 40 orders with the plant kibbler under 40 ℃ of temperature, take by weighing 10kg, place extractor, feeding CO
2, carry out supercritical extraction, extraction temperature is 40 ℃, and extracting pressure is 40MPa, and the time is 60min, CO
2Flow is 25L/h, removes most of lipid material, is that 1:15 mixes with residue after the extraction degreasing and zero(ppm) water by solid-to-liquid ratio; Lixiviate 60min in 100 ℃ of hot water baths, cooled and filtered obtains filtrating; Filtrating is centrifugal at 2800rpm, obtains clear liquor, vacuum concentration; Liquid concentrator mixes, stirs with 85% alcohol, centrifugal 25min, and the deposition drying obtains Morinda Citifolia fruit polysaccharide 142.2g.
Claims (3)
1. from the Morinda Citifolia fruit, extract the method for water-soluble polysaccharide, it is characterized in that may further comprise the steps:
(1) drying and crushing: with fresh Morinda Citifolia really clean up dry after, put in the baking oven that the oven dry back is crushed to the 40-60 order with the plant kibbler under 40-70 ℃ of temperature;
(2) supercritical CO
2Fluid extraction degreasing: above-mentioned Morinda Citifolia fruit powder is placed extractor, feed CO
2, carry out supercritical extraction, remove most of lipid material;
(3) lixiviate: the residue and the zero(ppm) water that will extract after the degreasing are that 1:15-20 mixes by solid-to-liquid ratio, lixiviate 30-120min in 80-100 ℃ of hot water bath, and cooled and filtered obtains filtrating;
(4) alcohol precipitation: it is centrifugal to filtrate, and obtains clear liquor, vacuum concentration, and liquid concentrator mixes, stirs with 85% alcohol, centrifugal 20-30min, the deposition drying obtains Morinda Citifolia fruit polysaccharide.
2. the method for from the Morinda Citifolia fruit, extracting water-soluble polysaccharide according to claim 1, it is characterized in that: the supercritical extraction temperature is 35-60 ℃ in the said step (2), and extracting pressure is 28-45MPa, and the time is 40-70min, CO
2Flow is 20L/h-35L/h.
3. the method for from the Morinda Citifolia fruit, extracting water-soluble polysaccharide according to claim 1, it is characterized in that: centrifugal rotational speed is 2800-3100rpm in the said step (4).
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703217A (en) * | 2012-06-04 | 2012-10-03 | 云南烟草科学研究院 | Noni extract and application thereof |
| CN102894366A (en) * | 2012-11-01 | 2013-01-30 | 中国医学科学院药用植物研究所云南分所 | Rubiaceae morinda citrifolia powder with anti-fatigue effect and preparation method thereof |
| CN103626886A (en) * | 2013-11-20 | 2014-03-12 | 中国农业科学院农产品加工研究所 | Method for extracting cereal seed coat active polysaccharide through assisted treatment of hot-pressing steam spray explosion method |
| CN103936874A (en) * | 2014-03-26 | 2014-07-23 | 刘凯 | Method for extracting sea cucumber polysaccharide from sea cucumber boiled juice |
| CN105495208A (en) * | 2015-12-14 | 2016-04-20 | 魏世平 | Organic noni healthy beverage and preparation method thereof |
| CN105533753A (en) * | 2015-12-29 | 2016-05-04 | 徐州市铜山区生命科学研究院 | Medicinal and edible microcirculation obstacle preventing and curing compound nutrient |
| CN106749746A (en) * | 2017-02-27 | 2017-05-31 | 云南省热带作物科学研究所 | It is a kind of to improve the method and extract obtained and application for obtaining the beautiful polysaccharide of Noni fruit promise |
| CN112010992A (en) * | 2020-09-08 | 2020-12-01 | 于淼 | Noni homogeneous polysaccharide extraction and separation method and application |
-
2011
- 2011-09-08 CN CN2011102648320A patent/CN102382202A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703217A (en) * | 2012-06-04 | 2012-10-03 | 云南烟草科学研究院 | Noni extract and application thereof |
| CN102894366A (en) * | 2012-11-01 | 2013-01-30 | 中国医学科学院药用植物研究所云南分所 | Rubiaceae morinda citrifolia powder with anti-fatigue effect and preparation method thereof |
| CN103626886A (en) * | 2013-11-20 | 2014-03-12 | 中国农业科学院农产品加工研究所 | Method for extracting cereal seed coat active polysaccharide through assisted treatment of hot-pressing steam spray explosion method |
| CN103626886B (en) * | 2013-11-20 | 2015-12-30 | 中国农业科学院农产品加工研究所 | A kind of hot pressing steam sprays the method that quick-fried method auxiliary treatment extracts cereal seed coat active polysaccharide |
| CN103936874A (en) * | 2014-03-26 | 2014-07-23 | 刘凯 | Method for extracting sea cucumber polysaccharide from sea cucumber boiled juice |
| CN103936874B (en) * | 2014-03-26 | 2016-03-02 | 北京灵净泉生物科技有限公司 | Boiled sea cucumber juice extracts the method for sea cucumber polysaccharide |
| CN105495208A (en) * | 2015-12-14 | 2016-04-20 | 魏世平 | Organic noni healthy beverage and preparation method thereof |
| CN105533753A (en) * | 2015-12-29 | 2016-05-04 | 徐州市铜山区生命科学研究院 | Medicinal and edible microcirculation obstacle preventing and curing compound nutrient |
| CN105533753B (en) * | 2015-12-29 | 2018-03-30 | 徐州市铜山区生命科学研究院 | A kind of compound nutritional agent of medicine-food two-purpose preventing and treating microcirculation disorder |
| CN106749746A (en) * | 2017-02-27 | 2017-05-31 | 云南省热带作物科学研究所 | It is a kind of to improve the method and extract obtained and application for obtaining the beautiful polysaccharide of Noni fruit promise |
| CN106749746B (en) * | 2017-02-27 | 2019-06-14 | 云南省热带作物科学研究所 | It is a kind of to improve the method for obtaining the beautiful polysaccharide of Noni fruit promise and extract obtained and application |
| CN112010992A (en) * | 2020-09-08 | 2020-12-01 | 于淼 | Noni homogeneous polysaccharide extraction and separation method and application |
| CN112010992B (en) * | 2020-09-08 | 2022-06-07 | 于淼 | Noni homogeneous polysaccharide extraction and separation method and application |
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Application publication date: 20120321 |