CN102353418A - Device for determining quantity of carbon dioxide generated by degrading organic matters with aerobic microorganisms - Google Patents
Device for determining quantity of carbon dioxide generated by degrading organic matters with aerobic microorganisms Download PDFInfo
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- CN102353418A CN102353418A CN201110246596XA CN201110246596A CN102353418A CN 102353418 A CN102353418 A CN 102353418A CN 201110246596X A CN201110246596X A CN 201110246596XA CN 201110246596 A CN201110246596 A CN 201110246596A CN 102353418 A CN102353418 A CN 102353418A
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Abstract
The invention provides a device for determining quantity of carbon dioxide generated by degrading organic matters with aerobic microorganisms. The device comprises an airtight reactor and a carbon dioxide determining recorder, wherein the airtight reactor comprises an opening, a sealing device and an exhaust device, the opening is formed on the top of the airtight reactor and is communicated with the outside, the sealing device is arranged in the opening which is formed on the top of the airtight reactor and is communicated with the outside, the sealing device is used for isolating the interior of the airtight reactor from the outside, and the exhaust device is connected with the carbon dioxide determining recorder; and an aerating device is arranged at the bottom of the airtight reactor. A determining environment of the device provided by the invention is approximate to an actual operating environment; and a determining principle is reliable, and the quantity of the carbon dioxide generated by degrading the organic matters with the aerobic microorganisms can be determined directly.
Description
Technical field
The present invention relates to a kind of device of measuring the carbon dioxide generating amount, especially a kind of device of measuring aerobic degradation organism carbon dioxide generating amount.
Background technology
Biological wastewater treatment technology with its consume less, efficient is high, cost is low, the technological operation convenient management is reliable and remarkable advantage such as non-secondary pollution and enjoy people's favor.The waste water aerobic biological treatment is a kind of treatment process commonly used; The active sludge of being made up of aerobic microbiological is exactly a kind of material commonly used; Being determined in the engineering of sludge activity is the important indicator that characterizes operation conditions; Mainly reflect now by organic clearance in the waste water; Though aforementioned detection method can be indicated sludge activity indirectly; But implied a hypothesis, that is: the organism of getting rid of has been degraded by microorganisms fully.If organic removal is to remove the coefficient result of mechanism by microbial degradation and other, add charcoal treatment waste water simultaneously as adopting, then the wastewater through organic matter clearance can't reflect sludge activity.At present, lack direct laboratory facilities quantitative measurement active sludge degradation of organic substances ability.
In addition, organic biodegradable amount also is an important parameters in the waste water treatment engineering design in the waste water, at present, mainly weighs through five-day BOD.The five-day BOD minute is long, and condition determination and actual treatment service condition differ greatly, and causes engineering design and actual motion effect to differ bigger.Need find directly, accurate means is measured can be by the organism amount of biological treatment degraded in the waste water, promptly biodegradable amount, rather than reflect indirectly through other parameters.
The oxygen quantity delivered also is an important parameters in the waste water treatment engineering design, and the oxygen quantity delivered need be confirmed according to the oxygen expenditure amount.The oxygen quantity delivered is not enough, can cause biochemical reaction to carry out not exclusively, and wastewater treatment efficiency is poor, and the oxygen quantity delivered is excessive, causes the waste of the energy, causes operating cost to improve.To various waste water quality, the oxygen expenditure amount is widely different.At present, because the assay method of oxygen expenditure amount lack of scientific, the oxygen quantity delivered mainly is determined empirically in the waste water treatment engineering design.
Solving above these key of problem is organic amounts of aerobic degradation in the direct assaying reaction device, but does not also have effective technical means to address the above problem at present.
Biological wastewater treatment is to utilize the vital movement of microorganism; Make the synthetic new protoplasm of an organic contaminant part that is solubilised state or colloidal state in the waste water; Be used for the growth of microorganism, another part is degraded to carbon dioxide, energy and water, thus a kind of disposal route that waste water is purified.The organism conversion process can be represented in order to following formula.
Can know by the organism conversion process; Carbon dioxide is a kind of product of organic matter degradation; So; Utilize the carbon dioxide generating amount; Can directly calculate the organic amount of microbial degradation; And then calculate the amount that the unit volume microorganism can degradation of organic substances, or organic biodegradable amount in the unit volume waste water, and the oxygen expenditure amount of biochemical reaction.
The carbon dioxide generating amount that can accurately measure biochemical reaction in a period of time is accurately to calculate the key of microbial degradation organism amount.
The Chinese invention patent application; Application number is: 200810150554.4; Publication number is CN101363825A; The open date is the name on February 11st, 2009 is called provides a kind of dynamically detecting carbon element loss of root system of plant in the application for a patent for invention Publication Specification of a " a kind of device of dynamically detecting carbon element loss of root system of plant " device; Can solve the problem of dynamically detecting carbon element loss of root system of plant; But; Because lack the limitation of aerating system and eudiometry method, this device can't be used for the direct mensuration of sewage disposal system aerobic degradation organic matter amount.
Summary of the invention
The purpose of this invention is to provide a kind of device of measuring aerobic degradation organism carbon dioxide generating amount; Through mensuration aerobic degradation organism carbon dioxide generating amount, thereby solve the problem that detects aerobic microbiological activity, organic biodegradable amount and biochemical reaction oxygen expenditure amount.
The present invention adopts the purity oxygen aeration, has eliminated the influence of Carbon Dioxide in Air; Adopt the pure nitrogen gas aeration, can obtain dissolving in the mixed liquor amount of depositing carbon dioxide, calculate this factor of elimination to measuring result's influence through difference; Through writing down the CO 2 measuring registering instrument of gas concentration lwevel, can obtain instant dense carbon dioxide degrees of data; Closed reactor has guaranteed that the carbon dioxide generating amount of measuring is whole amounts that microorganism discharges because of respiration in the closed reactor; Under near true ruuning situation, measure, eliminated of the influence of factors such as temperature microbial activity.So,, can obtain aerobic degradation organism carbon dioxide generating amount in a period of time through the integral and calculating of product in the minute section of air demand and gas concentration lwevel.
For realizing above-mentioned purpose, solution of the present invention is:
A kind of device of measuring aerobic degradation organism carbon dioxide generating amount; Comprise closed reactor and CO 2 measuring registering instrument; Said closed reactor top be provided with one with the opening of external communications, place the closed reactor top and opening external communications to make an inner packoff and the exhaust apparatus with external isolation of closed reactor, said exhaust apparatus is connected with said CO 2 measuring registering instrument; Said closed reactor bottom is provided with an aerator.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, its exhaust apparatus is a gas outlet.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, its gas outlet is connected through threeway with its CO 2 measuring registering instrument.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, it places the closed reactor top and opening external communications to make the inner packoff with external isolation of closed reactor is a rubber skin plug.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, its closed reactor bottom are provided with one and make the inner packoff with external isolation of closed reactor with the opening of external communications and the opening with external communications that places the closed reactor bottom.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, it places the opening with external communications of closed reactor bottom to make the inner packoff with external isolation of closed reactor is a rubber skin plug.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, its exhaust apparatus bottom connect an anti-foam and overflow device.
Said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount; Its closed reactor comprises the container body of top and open upper end; The top of going up of its closed reactor is located at container body top, and the upward top and the container body of this closed reactor adopt flange to be connected and seal.
The concrete method of application of this device is: microorganism to be measured and carrier thereof and nutrient matrix are put into closed reactor; The purity oxygen that adopts regime flow is from the reactor bottom aeration; Measure and record reactor head exhaust ports gas concentration lwevel; Utilize the generation of air demand and the gas concentration lwevel product integral and calculating carbon dioxide in the time period in minute length, carbon dioxide generating amount computing method are seen following formula (1) in the purity oxygen aeration time period.
W
O: the generation (mg) of carbon dioxide in the purity oxygen aeration time period
t
1: determinator brings into operation constantly
t
2: determinator finishes the time of running
Q
O: purity oxygen aeration flow (L/s)
C
t: certain is gas concentration lwevel (mg/L) constantly
Then, the reactor emptying is put into closed reactor with the parallel sample of another group equal volume microorganism to be measured and carrier and nutrient matrix.
Adopt the pure nitrogen gas aeration; Be consistent with purity oxygen aeration flow; From the reactor bottom aeration; Measure and record reactor head exhaust ports gas concentration lwevel; Utilize the generation of air demand and the gas concentration lwevel product integral and calculating carbon dioxide in the time period in minute length, carbon dioxide generating amount computing method are seen following formula (2) in the pure nitrogen gas aeration time period.
W
N: the generation (mg) of carbon dioxide in the pure nitrogen gas aeration time period
Q
N: pure nitrogen gas aeration flow (L/s)
Active sludge to be measured and waste water at a time between the section in a degradation of organic substances carbon dioxide generating amount can utilize following formula (3) to try to achieve.
W
B＝W
O-W
N
W
B: active sludge degradation of organic substances carbon dioxide generating amount (mg) in certain time period
The present invention can resolve the direct mensuration problem of oxygen animalcule degradation of organic substances carbon dioxide generating amount, and then can solve: the technical matters that runs in the mensuration of the mensuration of biodegradable organic amount and biochemical reaction oxygen expenditure amount in the mensuration of sludge activity, the waste water.
The present invention has following distinguishing feature:
At first, measure environment near the actual motion environment; Secondly, measuring principle is reliable, can directly measure aerobic degradation organism carbon dioxide generating amount.
The present invention can be used in the biological activity determination of mud in the various waste water treatment process in a large number, the aspects such as mensuration of the mensuration of organic biodegradable amount and biochemical reaction oxygen expenditure amount.Has using value at aspects such as environmental protection, biologies.
Description of drawings
Fig. 1 is the structural representation of first embodiment of the invention.
Fig. 2 is the structural representation of second embodiment of the invention.
Fig. 3 is the structural representation of third embodiment of the invention.
Embodiment
In order to make those skilled in the art person understand technical scheme of the present invention better, the present invention is done further detailed description below in conjunction with accompanying drawing and embodiment.
As shown in Figure 1; A kind of device of measuring aerobic degradation organism carbon dioxide generating amount; Comprise closed reactor 1 and CO 2 measuring registering instrument 2; Said closed reactor 1 top be provided with one with the opening 13 of external communications, place the closed reactor top and opening external communications to make an inner packoff 11 and the exhaust apparatus 12 with external isolation of closed reactor, said exhaust apparatus 12 is connected with said CO 2 measuring registering instrument 2; Said closed reactor 1 bottom is provided with an aerator 4.
This a kind of device of measuring aerobic degradation organism carbon dioxide generating amount, detect aerobic microbiological at a time between in the section during degradation of organic substances carbon dioxide generating amount, can implement according to following steps:
1, under normal temperature condition; Microorganism to be measured and carrier thereof and nutrient matrix are put into closed reactor 1 through the opening 13 that is arranged on closed reactor 1 top, and the amount that microorganism and carrier thereof and nutrient matrix are put into closed reactor 1 is no more than 2/3rds of closed reactor 1 volume.
2, through aerator 4; The purity oxygen that adopts regime flow is from closed reactor 1 bottom aeration; Through CO-2 analyser 2; Measure and write down the gas concentration lwevel of the exhaust apparatus discharge at closed reactor 1 top, utilize the generation W of the interior carbon dioxide of integral and calculating time period of air demand and gas concentration lwevel product
O
3, the opening 13 through being arranged on closed reactor 1 top is put into closed reactor 1 with material emptying in the closed reactor 1 with parallel sample of another group equal volume microorganism to be measured and carrier and nutrient matrix.
4, through aerator 4; The pure nitrogen gas that adopts regime flow is from closed reactor 1 bottom aeration; Measure and write down the gas concentration lwevel of exhaust apparatus 12 discharges at closed reactor 1 top through CO-2 analyser 2, utilize the generation W of the interior carbon dioxide of integral and calculating time period of air demand and gas concentration lwevel product
N
5, through formula (3), can obtain aerobic degradation organism carbon dioxide generating amount W
B
Be illustrated in figure 2 as the structural representation of the embodiment of the invention 2; It is improvement to embodiments of the invention 1; Device in Fig. 1; Be through being arranged on the opening 13 at closed reactor 1 top; This closed reactor is put into and discharged to microorganism and carrier thereof and nutrient matrix; Like this when discharging the material of closed reactor; Need whole closed reactor 1 is inverted; Adopt this mode; Increased workload; Also inconvenient, for ease with the material emptying in the closed reactor 1, be provided with an opening 15 in the bottom of closed reactor 1; And with 14 sealings of rubber skin plug; After experiment finishes like this, just can utilize action of gravity easily with material emptying in the closed reactor 1 through pulling out rubber piston 14.
Be illustrated in figure 3 as the structural representation of the embodiment of the invention 3; It is further improvement to embodiments of the invention 2; Device among the embodiment 2 is in operational process; Possibly have foam in the closed reactor 1 and get in the CO 2 measuring registering instrument 2, can damage CO 2 measuring registering instrument 2 like this through gas outlet.Get into CO 2 measuring registering instrument 2 for fear of foam, at exhaust pipe lower part an anti-foam is installed and is overflowed device 5.Should prevent that it was the container that a middle part has some circular holes that foam overflows device, the effect of circular hole is that supplied gas is passed through, and this container bottom tilts, and lowermost end is opened leakage fluid dram at the bottom angled place.
The above only is preferred embodiment of the present invention, not in order to restriction the present invention, all any modifications of within spirit of the present invention and principle, being done, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. device of measuring aerobic degradation organism carbon dioxide generating amount; Comprise closed reactor and CO 2 measuring registering instrument; It is characterized in that; Said closed reactor top be provided with one with the opening of external communications, place the closed reactor top and opening external communications to make an inner packoff and the exhaust apparatus with external isolation of closed reactor, said exhaust apparatus is connected with said CO 2 measuring registering instrument; Said closed reactor bottom is provided with an aerator.
2. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 1, it is characterized in that said exhaust apparatus is a gas outlet.
3. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 2, it is characterized in that said gas outlet is connected through threeway with said CO 2 measuring registering instrument.
4. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 1; It is characterized in that placing the closed reactor top and opening external communications to make the inner packoff with external isolation of closed reactor is a rubber skin plug.
5. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 1; It is characterized in that said closed reactor bottom is provided with one and makes the inner packoff with external isolation of closed reactor with the opening of external communications and the opening with external communications that places the closed reactor bottom.
6. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 5; It is characterized in that placing the opening with external communications of closed reactor bottom to make the inner packoff with external isolation of closed reactor is a rubber skin plug.
7. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 1, it is characterized in that described exhaust apparatus bottom connects an anti-foam and overflows device.
8. according to the said a kind of device of measuring aerobic degradation organism carbon dioxide generating amount of claim 1; It is characterized in that; Described closed reactor comprises the container body of top and open upper end; The top of going up of said closed reactor is located at container body top, and the upward top and the container body of this closed reactor adopt flange to be connected and seal.
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| CN201110246596XA CN102353418A (en) | 2011-08-26 | 2011-08-26 | Device for determining quantity of carbon dioxide generated by degrading organic matters with aerobic microorganisms |
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| CN201110246596XA CN102353418A (en) | 2011-08-26 | 2011-08-26 | Device for determining quantity of carbon dioxide generated by degrading organic matters with aerobic microorganisms |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6143515A (en) * | 1997-10-09 | 2000-11-07 | Saida Ironworks Co., Ltd. | Process for evaluating microbial-degradability of organic matters and apparatus therefor |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN201543645U (en) * | 2009-11-19 | 2010-08-11 | 中国矿业大学(北京) | Organic contaminated soil bioremediation reactor for quantitatively analyzing pollutant removal process |
| CN202177616U (en) * | 2011-08-26 | 2012-03-28 | 崔延瑞 | Device for measuring carbon dioxide quantity generated during degradation of organic substance by aerobic microorganism |
-
2011
- 2011-08-26 CN CN201110246596XA patent/CN102353418A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6143515A (en) * | 1997-10-09 | 2000-11-07 | Saida Ironworks Co., Ltd. | Process for evaluating microbial-degradability of organic matters and apparatus therefor |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN201543645U (en) * | 2009-11-19 | 2010-08-11 | 中国矿业大学(北京) | Organic contaminated soil bioremediation reactor for quantitatively analyzing pollutant removal process |
| CN202177616U (en) * | 2011-08-26 | 2012-03-28 | 崔延瑞 | Device for measuring carbon dioxide quantity generated during degradation of organic substance by aerobic microorganism |
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Application publication date: 20120215 |