CN218879911U - Biodegradation test device - Google Patents

Biodegradation test device Download PDF

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CN218879911U
CN218879911U CN202223434196.4U CN202223434196U CN218879911U CN 218879911 U CN218879911 U CN 218879911U CN 202223434196 U CN202223434196 U CN 202223434196U CN 218879911 U CN218879911 U CN 218879911U
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华彬彬
鞠美庭
陈玉龙
王卫帅
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Nankai University
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Abstract

The utility model discloses a biodegradation testing device, relating to the field of environmental protection; comprises a fermentation part, a gas metering part, a gas supply and collection part and a data processing and process control part; the fermentation part is used for carrying out biodegradation fermentation; the gas metering part is used for obtaining the volume of gas generated or consumed by fermentation and measuring the concentration of the gas; the gas supply and collection part supplies/collects gas consumed or produced by the microorganisms; the data processing and process control part is used for reflecting process data acquisition and calculating and storing a test result according to the collected data. The utility model provides a biodegradable testing arrangement not only can carry out anaerobic fermentation, also can carry out aerobic fermentation, and application scope is wide, but automatic real-time supervision, but the during operation unmanned on duty.

Description

Biodegradation test device
Technical Field
The utility model relates to an environmental protection technical field especially relates to a biodegradable testing arrangement.
Background
The microorganism degrades and converts organic waste and pollutants through anaerobic fermentation, aerobic fermentation and other reactions, and the accurate measurement of the amount of gas generated or oxygen consumed in the microbial degradation process has great significance for researching the microbial degradation reaction.
The anaerobic fermentation technology is one of effective ways for realizing resource utilization of biomass wastes. Organic matters in organic waste such as organic waste water, kitchen garbage, livestock and poultry manure, straws, energy crops and the like are converted into methane. The biogas is a mixed gas and comprises gases such as methane, carbon dioxide, water vapor, hydrogen sulfide, carbon monoxide, hydrogen, nitrogen, ammonia, oxygen and the like. The existing automatic testing device washes the methane with alkali, and measures the volume of the eluted gas as the amount of the generated methane. The existing method can not measure the whole and single components of the methane at the same time and can not show the whole degradation and gas production process of anaerobic fermentation microorganisms in detail. The gas which cannot be absorbed by the alkaline solution in the biogas comprises carbon monoxide, hydrogen, nitrogen, ammonia, oxygen and the like besides methane. The process therefore theoretically overestimates methane production.
The existing anaerobic biodegradation testing device mainly uses a fermentation bottle with fixed volume for fermentation. In a fermentation flask, a portion of the headspace volume is typically retained and filled with an inert gas (e.g., nitrogen) to cause an overestimation of gas production at the early stage of the fermentation reaction, and a compensation correction calculation underestimates total gas production; after the anaerobic fermentation is started, the tightness of the device is difficult to check, gas leakage is difficult to find in time, and gas generation loss is easily caused; the carbon dioxide generated by the absorption of the ectopic alkali liquor has large difference of absorption efficiency, and especially the absorption efficiency of the alkali liquor to the carbon dioxide at the later stage of fermentation is reduced, thus causing larger measurement error; in the existing gas measurement technology, such as a gas-liquid displacement method, a flap-type liquid displacement principle is utilized, when a flap is lifted, the water pressure applied to gas in a pipeline is reduced, and partial gas which is not measured escapes, so that the measurement error is obviously increased.
The aerobic microbial degradation capacity can be determined by the volume of oxygen consumed, by the substrate consumption or by the composition of the intermediate and final products of the different microbial processes. The existing aerobic biodegradation test technology mainly measures the degradation capacity of aerobic fermentation microorganisms by absorbing alkaline substances and indirectly measuring the amount of generated carbon dioxide, the measurement process is complex, and the accuracy is limited by the adsorption efficiency.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a biodegradable testing device to solve the problem that above-mentioned prior art exists, not only can carry out anaerobic fermentation, also can carry out aerobic fermentation, application scope is wide, but automatic real-time supervision, but the during operation unmanned on duty.
In order to achieve the above object, the utility model provides a following scheme:
the utility model provides a biodegradation testing device, which comprises a fermentation part, a gas metering part, a gas supply and collection part and a data processing and process control part; the fermentation part is used for carrying out biodegradation fermentation; the gas metering part is used for obtaining the volume of gas generated or consumed by fermentation and determining the concentration of the gas; the gas supply and collection part supplies/collects gas consumed or produced by the microorganisms; the data processing and process control part is used for acquiring reaction process data, and calculating and storing a test result according to the collected data.
Optionally, the fermentation part comprises a constant-temperature water bath, a fermentation bag and a stirring device; one side of the bottom of the constant-temperature water bath is communicated with a water distributor, one side of the upper part of the constant-temperature water bath is provided with an overflow port, and the water distributor and the overflow port are respectively communicated with an external heating device through hoses; the fermentation bag is arranged in the constant-temperature water bath through a tray, an opening is formed in the center of one side of the fermentation bag, and the opening is externally connected with the gas metering part and the gas supplying and collecting part; the stirring device comprises a magnetic stirrer arranged in the fermentation bag, and a magnetic base capable of continuously rotating a magnetic pole is arranged at the center of the bottom of the tray; the fermentation bag is characterized in that fixing clamps are arranged on two sides of the tray, and the fermentation bag is arranged on the tray through the fixing clamps.
Optionally, the gas metering section comprises a pressure sensor and a gas composition measuring sensor; a connecting rod is fixedly connected to the central position of the bottom of the tray, and a balancing weight is fixedly connected to the connecting rod after the connecting rod penetrates through the bottom of the constant-temperature water bath; the pressure sensor is arranged at the bottom of the balancing weight, and the stress surface of the pressure sensor and the balancing weight can be correspondingly buckled; the opening is connected with a gas composition measuring sensor and a gas storage container in sequence through an exhaust pipe, a valve is arranged on the gas inlet pipe, and the valve is a one-way valve.
Optionally, the bottom in the constant temperature water bath is provided with a telescopic sleeve, and the connecting rod passes through the telescopic sleeve and the bottom of the constant temperature water bath and then is connected with the balancing weight.
Optionally, the gas supply and collection part comprises the gas storage container, the gas storage container is a gas collection bag, the exhaust pipe is provided with an exhaust pump, and the exhaust pump is a gas pump or a peristaltic pump; the gas supply and collection part also comprises a gas supply container which is an air supply bag, the air supply bag is communicated with the opening through an air inlet pipe, the air inlet pipe is provided with a valve and an air inlet pump, the valve is a one-way valve, and the air inlet pump is an air pump or a peristaltic pump; the air inlet pipe and the exhaust pipe are connected with the opening through telescopic pipes, the bottoms of the telescopic pipes are connected with the opening through quick connectors, and balls are arranged in the quick connectors.
Optionally, the data processing and process control part comprises a PLC control system, and the PLC control system comprises a PLC controller, a data acquisition module, a screen display module, and a storage module; and the PLC is respectively connected with the pressure sensor, the gas composition measuring sensor, the magnetic base, the exhaust pump and the air inlet pump through control lines.
Optionally, a part of the water distributor, which is located in the constant-temperature water bath, is provided with a plurality of water outlet holes, the diameter of each water outlet hole of the water distributor is not more than 1mm, and each water outlet hole faces the bottom; the water for the water bath in the constant-temperature water bath tank is pure water with determined and constant density, and the fermentation bag is immersed in the water bath.
Optionally, the gas component measuring sensor's detection air chamber is connected with the blast pipe, and one side of detecting the air chamber is equipped with the reflector panel, and the opposite side is the transparent plate, and the distance between reflector panel and the transparent plate is fixed. The probe of the gas component measuring sensor is not in contact with gas, so that the influence of vapor in the gas on the gas component measuring sensor can be avoided.
Optionally, the force bearing surface of the pressure sensor and the balancing weight can be tightly buckled, and the sensitivity of the pressure sensor can be selected according to the fermentation volume or the test requirement, such as a 1000mL fermentation bag, and the sensitivity of the pressure sensor is selected to be accurate to 1g. The gas component sensor has gas chamber connected to the gas conduit, reflecting board in one side of the gas chamber, transparent board in the other side of the gas chamber, and fixed distance between the two boards. The sensor probe is not in contact with the gas, so that the influence of water vapor in the gas on the sensor is avoided. The PLC controller can set the upper and lower limits of the gas production volume for triggering the air pump or the peristaltic pump to start and stop air exhaust or the time for starting and stopping the air pump or the peristaltic pump according to requirements. The controller can display the curve of the gas production body along with the change of time in real time, and a built-in correction program can automatically compensate the discharged methane and correct the gas production volume to the volume under a standard state.
The utility model discloses the principle does: under the environment of an immersed water bath, the volume of the interior of the fermentation bag is changed due to the gas generated/consumed by the respiration of microorganisms, and then the buoyancy of the fermentation bag is increased or decreased, and the volume of the generated/consumed gas is obtained by converting buoyancy change data measured by a pressure sensor because the volume change and the buoyancy increase or decrease are in a determined relation; the PLC controller can periodically and automatically turn on and turn off the peristaltic pump according to the set gas production volume range or fermentation time, and the biogas in the fermentation bag is pumped out and conveyed to the gas component test sensor for component content measurement; the built-in correction program can compensate the volume of the discharged methane to the accumulated gas production volume, and meanwhile, the gas production volume can be corrected to the gas production volume in a standard state according to the data recorded by the temperature and pressure sensor, so that the total gas production/consumed gas volume and the change curve of the volume of specific components in the gas production/consumed gas volume along with time can be obtained.
The utility model also provides a biodegradable testing method, including following step:
starting an external heating device, setting the required water bath temperature, and conveying water bath water with the set temperature to the constant-temperature water bath tank;
filling the prepared fermentation raw materials into a fermentation bag, putting a magnetic stirrer, and removing air remained in the bag; connecting a quick connection port of the fermentation bag with a telescopic pipe, starting magnetic stirring, setting a required rotating speed, and starting a pressure sensor to record the real-time change condition of the gas production volume;
setting an upper limit value and a lower limit value of gas production volume for triggering the gas inlet pump and the gas exhaust pump to start gas supply and exhaust or setting a time interval value of gas supply/exhaust by the PLC controller, periodically adding/exhausting gas in the fermentation bag, and measuring gas components by the gas component measuring sensor;
the PLC control system collects and records data of an atmospheric pressure sensor, a gravity sensor and a gas composition measuring sensor; the built-in program calculates the volume data of the gas which is periodically supplied/discharged, converts the volume of the gas into the volume under the standard state according to the recorded atmospheric pressure, obtains the change curve of the volume of the gas under the standard state and the fermentation time, and the change curve of the volume of a certain gas component to the fermentation time, and further obtains the change conditions of the gas production rate, the methane production rate and the biodegradation rate.
Optionally, the gas production rate comprises calculating the gas production rate Y at 0 ℃ under standard atmospheric pressure by the following formula t The unit is NmL/g VS or Nml/g COD:
Figure BDA0004010554110000041
or
Figure BDA0004010554110000042
In the formula:
P side survey Indicates the atmospheric pressure measured by the atmospheric pressure sensor in kPa;
p represents standard atmospheric pressure and is 1.01325X 10 5 Pa;
m represents the number of times of discharging gas by the gas pump/peristaltic pump, and m is more than or equal to 0;
V stator Represents the set volume of gas discharged each time, and the unit is mL;
rho represents the density of the purified water in the water bath and is 1g/mL;
g represents a gravity constant of 9.8N/kg;
F t the buoyancy calculated by converting the value measured by the pressure sensor at t time is represented by the unit of N;
F 0 the buoyancy force is obtained by calculating the value measured by the pressure sensor after the fermentation is finished and the methane is discharged, and the unit is N;
T 0 denotes the Kelvin temperature at 0 ℃,273.15K;
t represents the temperature value of fermentation, such as T =37 when the fermentation is carried out at 37 ℃;
VS represents the volatile solid added in the fermentation system and has the unit of g;
COD represents the total chemical oxygen demand of sewage/wastewater added in the fermentation physique and is given in g;
the methane production rate comprises calculating the total volume V of methane production by the following formula Methane The unit is mL:
when the number of times m =0 that the air pump/peristaltic pump discharges the air,
Figure BDA0004010554110000051
/>
when the times m of discharging the gas by the gas pump/peristaltic pump is more than or equal to 1,
Figure BDA0004010554110000052
in the formula:
m represents the times of discharging gas by the gas pump/peristaltic pump, and m is more than or equal to 0;
V stator Represents the set volume of gas discharged per time in mL;
rho represents the density of the purified water in the water bath and is 1g/mL;
g represents a gravity constant of 9.8N/kg;
f represents buoyancy calculated by converting the numerical value measured by the pressure sensor t time, and the unit is N;
F 0 the buoyancy force is calculated by the numerical value measured by the pressure sensor after the biogas is discharged after the fermentation is finished, and the unit is N;
C m and represents the methane concentration in% of the gas pump/peristaltic pump discharge gas measured by the sensor.
The methane production rate Y at 0 ℃ under standard atmospheric pressure was calculated by the following formula CH4 The unit is NmL/g VS or Nml/g COD:
Figure BDA0004010554110000061
or->
Figure BDA0004010554110000062
In the formula:
V CH4 expressing the total volume of the produced methane, and the unit is mL;
P measuring Indicates the atmospheric pressure measured by the atmospheric pressure sensor in kPa;
p represents standard atmospheric pressure and is 1.01325X 10 5 Pa;
T 0 Denotes the Kelvin temperature at 0 ℃,273.15K;
t represents the temperature value of fermentation, such as T =37 ℃ if the fermentation is carried out at 37 ℃;
VS represents the volatile solid added in the fermentation system and has the unit of g;
COD represents the total chemical oxygen demand of sewage/wastewater added in the fermentation physique and is given in g;
when the biochemical methane production potential and the anaerobic sludge to methane activity are calculated, the result of the blank control needs to be subtracted from the result obtained in the test;
the biodegradation rate includes the production of CO calculated by the following formula 2 Total volume V CO2 The unit is mL:
when the number of times m =0 that the air pump/peristaltic pump discharges the air,
Figure BDA0004010554110000063
when the times m of discharging the gas by the gas pump/peristaltic pump is more than or equal to 1,
Figure BDA0004010554110000064
in the formula:
m represents the number of times of discharging gas by the gas pump/peristaltic pump, and m is more than or equal to 0;
V stator Represents the set volume of gas discharged per time in mL;
rho represents the density of the purified water in the water bath and is 1g/mL;
g represents a gravity constant of 9.8N/kg;
f represents buoyancy calculated by converting the value measured by the pressure sensor at t time, and the unit is N;
F 0 the buoyancy force is calculated by the numerical value measured by the pressure sensor after the biogas is discharged after the fermentation is finished, and the unit is N;
C m the concentration of carbon dioxide measured by the sensor of the gas pump/peristaltic pump exhaust gas is shown in unit percent;
the biodegradation rate D was calculated by the following formula t
Figure BDA0004010554110000071
/>
In the formula:
V CO2 indicating the production of CO 2 Total volume in mL;
V Blank indicating blank control production of CO 2 Total volume in mL;
TV CO2 indicates the theoretical CO generated by the complete degradation of the substrate 2 Total volume in mL.
The utility model discloses for prior art gain following technological effect:
the utility model discloses utilize the buoyancy principle to measure gas volume at fermentation bag normal position, avoided the dystopy to measure the underestimation or the overestimate that causes the result, no matter produce gas/consume gas speed fast slow, the homoenergetic is real-time, accurate measurement gas volume. In the processes of gas volume measurement and component concentration determination, no removal steps such as adsorption/elution/condensation/absorption and the like are adopted, so that overestimation or underestimation of a measurement value caused by difference of adsorption/elution/condensation/absorption efficiency is avoided, and nondestructive measurement is achieved. After measurement, the gas can be collected for further analysis. The fermentation bag is immersed in the water bath in the whole process, so that the air tightness can be checked at any time, and the gas leakage and the loss of substances in the whole process are avoided. For anaerobic fermentation, the device can simultaneously complete the measurement of methane, carbon dioxide and other components in the methane and the marsh gas. The fermentation bag with the variable volume can eliminate the influence of gas reserved in the headspace volume of the traditional fermentation tank on the result, so that the accumulated gas production curve can truly reduce the anaerobic fermentation gas production process. For aerobic fermentation, the gas is periodically supplied/exhausted according to set time, so that the device has the characteristic of breathing and is closer to the natural environment condition of the aerobic fermentation of microorganisms.
The utility model discloses continuous automatic measurement, the accuracy is high, and resolution ratio is high, for the analysis and research than methane production activity, biochemical methane production potentiality, biochemical oxygen demand, fermentation process such as microorganism breathing provide favourable help.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of the biodegradation testing device of the present invention;
FIG. 2 is a graph of the cumulative methane and methane volume over time for anaerobic fermentation;
description of reference numerals: 1-constant temperature water bath; 2-a heating device; 3-a water distributor; 4-an overflow port; 5-fermentation bag; 6-a tray; 7-a pressure sensor; 8-a connecting rod; 9-quick connection interface; 10-magnetic stirrer; 11-a retaining clip; 12-a magnetic base; 13-a telescopic sleeve; 14-a telescopic tube; 15-an exhaust pump; 16-floating beads; 17-an intake pump; 18-a gas composition measuring sensor; 19-a PLC control system; 20-control signal lines; 21-air collecting bag; 22-air supply bag; 23-valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model aims at providing a biodegradable testing device to solve the problem that above-mentioned prior art exists, not only can carry out anaerobic fermentation, also can carry out aerobic fermentation, application scope is wide, but automatic real-time supervision, but the during operation unmanned on duty.
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the following detailed description.
The first embodiment is as follows:
as shown in fig. 1, the present embodiment provides a biodegradation test device, which mainly comprises a fermentation portion, a gas metering portion, a gas supplying and collecting portion, and a data processing and process control portion. The fermentation part comprises a constant temperature water bath 1, an external heating device 2 and a fermentation bag 5, the bottom of the constant temperature water bath 1 is provided with a water distributor 3, and one side of the top is provided with an overflow port 4; the heating device 2 is respectively connected with a water distributor 3 and an overflow port 4 of the constant temperature water bath 1 through hoses. The gas metering part comprises a tray 6 for fixing the fermentation bag 5, a pressure sensor 7 and a connecting rod 8 for connecting the tray 6 and the pressure sensor 7; the center of one side of the fermentation bag 5 is provided with a quick connection port 9 with a valve 23 and a floating bead 16, and a magnetic stirrer 10 is arranged in the bag; the two sides of the tray 6 are provided with fixing clamps 11, and the center is provided with a magnetic base 12 which can continuously rotate a magnetic pole; the top of the connecting rod 8 is fixedly connected with the center of the tray 6, the middle of the connecting rod penetrates through the bottom of the constant-temperature water bath 1 and is connected with the constant-temperature water bath through a telescopic sleeve 13, and the bottom of the connecting rod is fixedly connected with a balancing weight; the stress surface of the pressure sensor 7 and the balancing weight at the bottom of the connecting rod 8 can be correspondingly buckled; the biogas component measuring system comprises a telescopic pipe 14 and a gas component measuring sensor 18; the gas supply and collection part comprises a gas collection bag 21, a gas supply bag 22, a valve 23, an exhaust pump 15 and an air inlet pump 17, and is provided with an interface which can be connected with a quick connection interface at the upper part of the fermentation bag through a telescopic pipe; the gas outlet of the exhaust pump 15 is connected to a gas component measuring sensor 18; the PLC control system 19 and the accessory structure thereof comprise a PLC controller, a data acquisition module, a screen display module and a storage module; the PLC controller is respectively connected with the pressure sensor, the magnetic pole base, the peristaltic pump/air pump and the gas component measuring sensor through control signal lines 20.
The second embodiment:
this example provides a biodegradation test method as follows:
the testing device according to the first embodiment is used for anaerobic fermentation research, and a fermentation bag with the volume of 500mL, a pressure sensor with the measuring range of 500g, a methane component detection probe and a peristaltic pump with the flow rate of 60mL/m are selected. The specific operation steps comprise firstly, starting an external heating device in advance, setting the external heating device to be 37 ℃, and conveying purified water bath water with the temperature of 37 ℃ to a main water bath; then, the prepared anaerobic sludge with the concentration of 10gVSS/L, the load of 1.0g COD/gVSS and the volume of 300mL is filled into a fermentation bag, a magnetic stirrer is placed in the fermentation bag, a quick connection port of the fermentation bag is connected with a telescopic pipe, an exhaust valve and a peristaltic pump are started to remove air remained in the fermentation bag, the magnetic stirrer is started to set the rotating speed to be 120r/min, and a pressure sensor is started to record the real-time change condition of the gas production volume; reading and recording pressure sensor data every 15 minutes through a PLC (programmable logic controller), setting the upper limit value of a gas production volume for triggering a peristaltic pump to start exhaust to be 150mL and the lower limit value for stopping the peristaltic pump to be 50mL, and periodically discharging biogas generated in a fermentation bag to enter a methane gas measuring sensor for measuring and recording the content of methane in the biogas; and starting a built-in correction program of the PLC to replenish the periodically discharged gas production volume data to a corresponding time period, converting the gas production volume into the volume under the standard state according to the recorded atmospheric pressure and fermentation temperature to obtain a change curve of the total gas production volume under the standard state to the fermentation time, and obtaining a change curve of the component volume to the fermentation time according to the content of a certain component recorded by the gas component detector to further obtain the gas production rate change condition. Control experiments were performed in a XX brand methanogenic potential testing apparatus with a working volume of 500mL. The generated carbon dioxide is absorbed by using an ectopic alkali liquor, and methane is measured by using a flap-type liquid displacement principle.
The experimental result is as shown in figure 2, the utility model discloses the device can completely obtain two sets of data of marsh gas and methane that the microorganism produced among the anaerobic fermentation process, and the process of accurate reaction anaerobic fermentation microorganism gas production and methane production. Only one group of microorganisms in the control group produced methane data. The utility model discloses the whole and methane component of marsh gas are surveyd simultaneously to the device, and the data volume of obtaining is more, reacts anaerobic fermentation gas production process more comprehensively and more thoroughly and really. The utility model discloses the real-time on-line measuring pressure sensor of device changes the numerical value that produces, and data acquisition frequency is according to the time settlement, and is more intensive meticulous. The control device is used for measuring N in the headspace of the fermentation bottle 2 The effect of (b) was corrected for, but the methane content was still overestimated in the early stages of fermentation. CO at initial stage of fermentation 2 The proportion in the biogas is high, and a large amount of CO is eluted by alkali liquor 2 Failing to be fully absorbed, resulting in an overestimated amount of methane. In the later stage of fermentation, the accumulated methane quantity measured by the device of the utility model is higher than that of the contrast device. The headspace volume of the fermentation device is reduced to improve the anaerobic fermentation effectRate, the utility model discloses the device makes the headspace volume diminish through the exhaust of law, is favorable to improving the methane productivity.
In the description of the present invention, it should be noted that the terms "center", "top", "bottom", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The utility model discloses a concrete example is applied to explain the principle and the implementation mode of the utility model, and the explanation of the above example is only used to help understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.

Claims (4)

1. A biodegradation testing device is characterized in that: comprises a fermentation part, a gas metering part, a gas supply and collection part and a data processing and process control part; the fermentation part comprises a constant-temperature water bath, a fermentation bag and a stirring device; a water distributor is communicated with one side of the bottom of the constant-temperature water bath tank, an overflow port is formed in one side above the constant-temperature water bath tank, and the water distributor and the overflow port are respectively communicated with an external heating device through hoses; the fermentation bag is arranged in the constant-temperature water bath through a tray, an opening is formed in the center of one side of the fermentation bag, and the opening is externally connected with the gas metering part and the gas supplying and collecting part; the stirring device comprises a magnetic stirrer arranged in the fermentation bag, and a magnetic base capable of continuously rotating a magnetic pole is arranged at the center of the bottom of the tray; fixing clamps are arranged on two sides of the tray, the fermentation bag is mounted on the tray through the fixing clamps, and the fermentation part is used for performing biodegradation fermentation; the gas metering section includes a pressure sensor and a gas composition measuring sensor; a connecting rod is fixedly connected to the central position of the bottom of the tray, and a balancing weight is fixedly connected to the connecting rod after the connecting rod penetrates through the bottom of the constant-temperature water bath; the pressure sensor is arranged at the bottom of the balancing weight, and the stress surface of the pressure sensor can be correspondingly buckled with the balancing weight; the opening is sequentially connected with a gas component measuring sensor and a gas storage container through an exhaust pipe, a valve is arranged on the exhaust pipe and is a one-way valve, and the gas metering part is used for obtaining the volume of gas generated or consumed by fermentation and measuring the concentration of the gas; the gas supply and collection part comprises a gas storage container, the gas storage container is a gas collection bag, an exhaust pump is arranged on the exhaust pipe, and the exhaust pump is an air pump or a peristaltic pump; the gas supply and collection part also comprises a gas supply container which is an air supply bag, the air supply bag is communicated with the opening through an air inlet pipe, the air inlet pipe is provided with a valve and an air inlet pump, the valve is a one-way valve, and the air inlet pump is an air pump or a peristaltic pump; the gas supply and collection part supplies/collects gas consumed or generated by microorganisms; the data processing and process control part comprises a PLC control system, and the PLC control system comprises a PLC controller, a data acquisition module, a screen display module and a storage module; the PLC controller is respectively connected with the pressure sensor, the gas composition measuring sensor, the magnetic base, the exhaust pump and the air inlet pump through control lines, and the data processing and process control part is used for acquiring reaction process data and calculating and storing a test result according to the collected data.
2. The biodegradation testing device of claim 1, wherein: the bottom in the constant temperature water bath is provided with a telescopic sleeve, and the connecting rod passes through the telescopic sleeve and the bottom of the constant temperature water bath and then is connected with the balancing weight.
3. The biodegradation testing device of claim 1, wherein: the part of the water distributor, which is positioned in the constant-temperature water bath, is provided with a plurality of water outlets, the diameter of each water outlet of the water distributor is not more than 1mm, and the water outlets face the bottom; the water for the water bath in the constant-temperature water bath is pure water with determined and constant density, and the fermentation bag is immersed in the water bath.
4. The biodegradation testing device of claim 1, wherein: a detection gas chamber of the gas component measurement sensor is connected with the exhaust pipe, a reflecting plate is arranged on one side of the detection gas chamber, a transparent plate is arranged on the other side of the detection gas chamber, and the distance between the reflecting plate and the transparent plate is fixed; the probe of the gas component measuring sensor is not in contact with gas, so that the influence of vapor in the gas on the gas component measuring sensor can be avoided.
CN202223434196.4U 2022-12-21 2022-12-21 Biodegradation test device Active CN218879911U (en)

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