CN102349447A - Two-cutting embryo taking method for young grape fruits - Google Patents

Abstract

The invention discloses a two-cutting embryo taking method for young grape fruits. In a sterile operation table, the young grape fruit is sterilized by 75% ethanol; both hands are sterilized, the left hand tightly holds a carpopodium and a fruit stem base, and the right hand holds a scalpel to carry out transverse cutting for one circle from the middle about 2/5 position of a fruit grain near one side of the embryo, for the first cutting; the depth is not deep enough to hurt the embryo; the young grape fruit is lightly torn to clearly see the embryo insertion position; the second cutting is characterized by vertically cutting one time in half from the middle; and finally, an ovule is soaked out or picked out with a knife point. The method provided by the invention is suitable for taking the embryo before the grape fruit grain changes color and becomes soft, the pericarp instead of the young embryo is directly disinfected, and the method has the characteristics of no pollution, high ovule survival rate and high developmental rate. Efficiency is improved, resource is saved, and the method has wide applicability.

Description

Grape young fruit two cuttves are got the embryo method

Invention field

The present invention relates to the gardening biological technical field.Specifically, the present invention relates to the technical field that a kind of grape young fruit is got embryo.

Background technology

In the tradition grape embryo rescue techniques, the aseptic embryo of grape stripped two kinds of methods.The one, win the fruit grain; Rinse well with running water; Strip out ovule with aseptic tweezers and scalpel; Be positioned in the sterile working platform; Ovule is first with 70% ethanol disinfection, aseptic water washing; With the sterilization of 0.1% mercuric chloride, aseptic water washing, obtain aseptic embryo, but ovule survival rate and ovule developmental rate are low again.The 2nd, win young fruit; Wash with running water; Soak into the back once with 70% ethanol again with aseptic water washing; On superclean bench, pour immersion of 0.1% mercuric chloride and vibration into several times; Use rinsed with sterile water afterwards, in culture dish, the fruit grain is cut open, take out ovule with aseptic tweezers and scalpel; But it is not high to get embryo efficient, is prone to pollute.

Summary of the invention

Main purpose of the present invention is exactly that the ovule survival rate and the ovule developmental rate that exist to the method that strips that adopts the aseptic embryo of grape in present traditional grape embryo rescue techniques are low, and it is not high to get embryo efficient, is prone to the state of the art of pollution.

The present invention provides a kind of grape young fruit to get the embryo method, has characteristics such as pollution-free, ovule survival rate and developmental rate height, has improved efficient, has practiced thrift resource.

The present invention realizes through following technical scheme: through in the sterile working platform with grape young fruit 75% ethanol disinfection; The both hands sterilization; Left hand is firmly grasped carpopodium and fruit base portion; The right hand is held scalpel first cutter and is leaned on embryo one side fruit grain 2/5 cross cut one circle approximately from the middle part; The degree of depth does not injure embryo, gently breaks into two with one's hands with have gentle hands, sees the embryo inserted part clearly; The therefrom perpendicular partial application of second cutter is stained with ovule go out with point of a knife at last or is chosen into two.

The invention provides the method that a kind of grape young fruit two cuttves are got embryo, may further comprise the steps:

(1) the band handle is cut the grape young fruit, removes wounded or disabled, malformed fruit, under the running water that flows, rinses well;

(2) in the sterile working platform, fruit grain immersed be equipped with in the beaker of 75% ethanol, stir 10 circles with glass bar, with aseptic water washing 3 times, filter is dried for use slightly;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter first cutter and leaned on really grain 2/5 cross cut one circle of embryo one side from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, second cutter with the therefrom perpendicular partial application in scalpel middle part into two;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to ovule and grows medium.

Among the present invention, used vessel, apparatus and both hands all need be sterilized, and each bottle culture medium inoculated finishes and blade should be sterilized.

Through the concrete summary of the invention of embodiment of the present invention, can reach following effect:

Because of operation in aseptic; Sense inconvenience slightly; Method provided by the invention is suitable for the embryo of getting before the deliquescing of grape fruit annesl; Sterilization directly acts on pericarp but not rataria; The whole only point of a knife contact rataria of embryo process of getting, have pollution-free, the characteristics that ovule survival rate and developmental rate are high; Improve efficient generally, practiced thrift resource.

Embodiment

Below, lift embodiment the present invention is described, still, the present invention is not limited to following embodiment.In addition, in following explanation, if no special instructions, % all refers to the m/m mass percent.

All reagent, instrument and the instrument of selecting for use among the present invention all is well known selecting for use, but do not limit enforcement of the present invention, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.

Embodiment:

Through in the sterile working platform with grape young fruit 75% ethanol disinfection; The both hands sterilization; Left hand is firmly grasped carpopodium and fruit base portion; The right hand is held scalpel first cutter and is leaned on embryo one side fruit grain 2/5 cross cut one circle approximately from the middle part; The degree of depth does not injure embryo, gently breaks into two with one's hands with have gentle hands, sees the embryo inserted part clearly; The therefrom perpendicular partial application of second cutter is stained with ovule go out with point of a knife at last or is chosen into two.

The present invention is because of being employed in aseptic operation, sense inconvenience slightly, and this method is suitable for the embryo of getting before the deliquescing of grape fruit annesl, has pollution-freely, and the characteristics that ovule survival rate and developmental rate are high have improved efficient generally, have practiced thrift resource.Grape young fruit two cuttves are got the fruit that the embryo method also can be used for need not to sterilize with sterile working and are got embryo, and efficient is higher.

Embodiment:

(1) will be with handle to cut the grape young fruit, remove wounded or disabled, malformed fruit, under the running water that flows, rinse well;

(2) will fruit grain immerse and be equipped with in the beaker of 75% ethanol, place in the sterile working platform, stir 10 circles,, filter dried for use slightly with aseptic water washing 2-3 time with glass bar;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter first cutter and leaned on really grain 2/5 cross cut one circle of embryo one side from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, second cutter with the therefrom perpendicular partial application in scalpel middle part into two;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to ovule and grows medium.

Claims (1)

1. method that grape young fruit two cuttves are got embryo is characterized in that described method may further comprise the steps:

(1) the band handle is cut the grape young fruit, removes wounded or disabled, malformed fruit, under the running water that flows, rinses well;

(2) in the sterile working platform, fruit grain immersed be equipped with in the beaker of 75% ethanol, stir 10 circles with glass bar, with aseptic water washing 3 times, filter is dried for use slightly;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter first cutter and leaned on really grain 2/5 cross cut one circle of embryo one side from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, second cutter with the therefrom perpendicular partial application in scalpel middle part into two;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to ovule and grows medium.