CN102349447B - Two-cutting embryo taking method for young grape fruits - Google Patents

Abstract

The invention discloses a two-cutting embryo taking method for young grape fruits. In a sterile operation table, the young grape fruit is sterilized by 75% ethanol; both hands are sterilized, the left hand tightly holds a carpopodium and a fruit stem base, and the right hand holds a scalpel to carry out transverse cutting for one circle from the middle about 2/5 position of a fruit grain near one side of the embryo, for the first cutting; the depth is not deep enough to hurt the embryo; the young grape fruit is lightly torn to clearly see the embryo insertion position; the second cutting is characterized by vertically cutting one time in half from the middle; and finally, an ovule is soaked out or picked out with a knife point. The method provided by the invention is suitable for taking the embryo before the grape fruit grain changes color and becomes soft, the pericarp instead of the young embryo is directly disinfected, and the method has the characteristics of no pollution, high ovule survival rate and high developmental rate. Efficiency is improved, resource is saved, and the method has wide applicability.

Description

Grape young fruit two cuttves are got the embryo method

Invention field

The present invention relates to the gardening biological technical field.Specifically, the present invention relates to the technical field that a kind of grape young fruit is got embryo.

Background technology

In the tradition grape embryo rescue techniques, the aseptic embryo of grape stripped two kinds of methods.The one, win the fruit grain, rinse well with running water, strip out ovule with aseptic tweezers and scalpel, be positioned in the aseptic operating platform, ovule is used first 70% ethanol disinfection, aseptic water washing, with the sterilization of 0.1% mercuric chloride, aseptic water washing, obtain aseptic embryo, but ovule survival rate and Ovule Development rate are low again.The 2nd, win young fruit, wash with running water, rear with aseptic water washing once with the infiltration of 70% ethanol again, superclean bench pour that 0.1% mercuric chloride soaks into and vibration several times, use afterwards rinsed with sterile water, the fruit grain is cut open in culture dish with aseptic tweezers and scalpel, take out ovule, but it is not high to get embryo efficient, easily pollutes.

Summary of the invention

Main purpose of the present invention is exactly that the ovule survival rate and the Ovule Development rate that exist for the method that strips that adopts the aseptic embryo of grape in present traditional grape embryo rescue techniques are low, and it is not high to get embryo efficient, the state of the art of easily polluting.

The invention provides a kind of grape young fruit and get the embryo method, have pollution-freely, ovule survival rate and developmental rate high have improved efficient, have saved resource.

The present invention is achieved through the following technical solutions: by in aseptic operating platform with grape young fruit 75% ethanol disinfection, the both hands sterilization, left hand is firmly grasped carpopodium and fruit base portion, the right hand is held scalpel the first cutter and is leaned on approximately fruit grain 2/5 cross cut one circle of embryo one side from the middle part, the degree of depth does not injure embryo, gently breaks into two with one's hands with have gentle hands, sees the embryo inserted part clearly, the second cutter therefrom perpendicular partial application is stained with ovule go out with point of a knife at last or is chosen into two.

The invention provides the method that a kind of grape young fruit two cuttves are got embryo, may further comprise the steps:

(1) the band handle is cut the grape young fruit, removes wounded or disabled, malformed fruit, rinses well under the running water that flows;

(2) in aseptic operating platform, fruit grain immersed be equipped with in the beaker of 75% ethanol, stir 10 circles with glass bar, with aseptic water washing 3 times, slightly be filtered dry stand-by;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter the first cutter and leaned on embryo one side fruit grain 2/5 cross cut one circle from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, the second cutter therefrom erects partial application into two with the scalpel middle part;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to the Ovule Development medium.

Among the present invention, used vessel, apparatus and both hands all need be sterilized, and each bottle culture medium inoculated is complete should sterilize blade.

By implementing the concrete summary of the invention of the present invention, can reach following effect:

Because of operation in aseptic, slightly sense inconvenience, method provided by the invention is suitable for the embryo of getting before the deliquescing of grape fruit annesl, sterilization directly acts on pericarp but not rataria, the whole only point of a knife contact rataria of embryo process of getting, have pollution-free, the characteristics that ovule survival rate and developmental rate are high, improve generally efficient, saved resource.

Embodiment

Below, for embodiment the present invention is described, still, the present invention is not limited to following embodiment.In addition, in following explanation, if no special instructions, % all refers to the m/m mass percent.

All reagent, instrument and the instrument of selecting among the present invention all is well known selecting, but do not limit enforcement of the present invention, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.

Embodiment:

By in aseptic operating platform with grape young fruit 75% ethanol disinfection, the both hands sterilization, left hand is firmly grasped carpopodium and fruit base portion, the right hand is held scalpel the first cutter and is leaned on approximately fruit grain 2/5 cross cut one circle of embryo one side from the middle part, the degree of depth does not injure embryo, gently breaks into two with one's hands with have gentle hands, sees the embryo inserted part clearly, the second cutter therefrom perpendicular partial application is stained with ovule go out with point of a knife at last or is chosen into two.

The present invention is because adopting operation in aseptic, slightly sense inconvenience, and the method is suitable for the embryo of getting before the deliquescing of grape fruit annesl, has pollution-freely, and the characteristics that ovule survival rate and developmental rate are high have improved efficient generally, have saved resource.Grape young fruit two cuttves get that the embryo method also can be used for need not to sterilize and the fruit of sterile working is got embryo, and efficient is higher.

Embodiment:

(1) will be with handle to cut the grape young fruit, remove wounded or disabled, malformed fruit, under the running water that flows, rinse well;

(2) really the grain immersion is equipped with in the beaker of 75% ethanol, places in the aseptic operating platform, stirs 10 circles with glass bar, uses aseptic water washing 2-3 time, slightly is filtered dry stand-by;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter the first cutter and leaned on embryo one side fruit grain 2/5 cross cut one circle from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, the second cutter therefrom erects partial application into two with the scalpel middle part;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to the Ovule Development medium.

Claims (1)

1. method that grape young fruit two cuttves are got embryo is characterized in that described method may further comprise the steps:

(1) the band handle is cut the grape young fruit, removes wounded or disabled, malformed fruit, rinses well under the running water that flows;

(2) in aseptic operating platform, fruit grain immersed be equipped with in the beaker of 75% ethanol, stir 10 circles with glass bar, with aseptic water washing 3 times, slightly be filtered dry stand-by;

(3) both hands are sterilized and are worn sterile gloves, and left hand is firmly grasped carpopodium and fruit base portion, and the right hand is held aseptic operation cutter the first cutter and leaned on embryo one side fruit grain 2/5 cross cut one circle from fruit grain middle part with the rear portion of blade, and the degree of depth does not injure embryo, with the hands breaks into two with one's hands gently;

(4) see the embryo inserted part clearly, the second cutter therefrom erects partial application into two with the scalpel middle part;

(5) with 1/5 fruit grain pericarp towards being placed down on the pallet, 1/5 fruit grain pinches with hand in addition, gently shells pulp with point of a knife, ovule is stained with or chooses, and directly with point of a knife ovule is seeded to the Ovule Development medium.