CN102342346A - Preparation method of Tuber dicum cook et Massee tea and Tuber dicum cook et Massee tea product - Google Patents
Preparation method of Tuber dicum cook et Massee tea and Tuber dicum cook et Massee tea product Download PDFInfo
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- CN102342346A CN102342346A CN2010102398208A CN201010239820A CN102342346A CN 102342346 A CN102342346 A CN 102342346A CN 2010102398208 A CN2010102398208 A CN 2010102398208A CN 201010239820 A CN201010239820 A CN 201010239820A CN 102342346 A CN102342346 A CN 102342346A
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Abstract
The invention relates to a preparation method of Tuber dicum cook et Massee tea, comprising the steps of: stoving washed Tuber dicum cook et Massee at a temperature of lower than or equal to 60DEG C to water weight content of less than or equal to 10%, crushing the Tuber dicum cook et Massee, and taking dried particles with a particle size of 5-30 meshes as the product. The product is a pure natural bacterial tea drink with dry Tuber dicum cook et Massee as the raw material and witout accessories. Pharmacodynamic experiments show that the product of the invention has a function of improving body immunity, and provides a new eating approach for utilizing Tuber dicum cook et Massee and giving full play to its medical values. With simple equipment and low cost, the method provided in the invention is easy to realize standardized production.
Description
Technical field
The present invention relates to a kind of preparation method and resulting bacterium tea product of mushroom tea.
Background technology
Ferfas (Tuber dicum cook et Massee) has another name called pig arch bacterium, no grain rattan fruit, Radix cynanchi wilfordii, truffle, be subordinate to Mycophytes door, Ascomycotina,
Tuberale,
Tuberaceae, Tuber.Ferfas has unique fragrance, mouthfeel and nutritive value, the history of the existing more than one thousand years of human consumption ferfas.America and Europe etc. country even claim that ferfas be " black diamond " is the superfine product in the wild bacterium, with caviar, Foie Gras same be called as three big treasures (fourth of the twelve Earthly Branches morning mist. [M], Chinese gill fungus bacterium, Beijing: Science Press, 2009).
Modern study confirms that in the main active of ferfas, the a-androstanol has the sexual health effect, and that ceramide and polysaccharide have is antitumor, adjusting immunity etc.; These active materials all be widely used in medicine, the foods and cosmetics (Tang Yajie etc. ferfas active component and artificial cultivation progress [J] thereof. Chinese herbal medicine, 2007,38 (4)).
China at present to the converted products of ferfas only just like limited several kinds of " truffle oil ", " ferfas sauce " etc. (Liu Hongyu etc. nutritive value of ferfas and development and use thereof [J]. development of resources and market, 1997,13 (2); CN1473505).Because being of high nutritive value of ferfas yields poorly, the huge market demand, research and development ferfas series products has very high economic worth.
Summary of the invention
To above-mentioned situation, the present invention is to provide a kind of preparation method of ferfas tea, and make the ferfas tea that obtains by this method.
The preparation method of ferfas tea of the present invention; Be in≤60 ℃ with the ferfas of cleaning; More preferably under 40 ℃～50 ℃ temperature conditions, dry (w) %, preferably (w) % to moisture≤9 to moisture≤10; After the pulverizing, the dried particles of getting particle diameter and be 5～30 mesh sieves (being equivalent to 4000～550 μ m) scope is as product.Result of the test shows that the bake out temperature in the manufacturing process is too high, and/or granularity excessive/too small, all can the mouthfeel when drinking have a significant effect.
The corresponding ferfas tea product that adopts the above-mentioned preparation method of the present invention to obtain, be a kind of be raw material with dried ferfas, do not add the bacterium tea-drinking article of the pure natural of auxiliary material, can be like the mode of conventional tea article such as green tea, black tea, drink after brewing with boiling water.Pharmacodynamic experiment shows that it has the effect that improves immunity of organisms; For the utilization of ferfas with give full play to its medical value a kind of new edible approach is provided, and equipment is simple, and cost is low; Be prone to realize standardized production, to satisfy the demand of market to all kinds of ferfas food.
Below again foregoing of the present invention is done further to specify through the specific embodiment of embodiment.But should this be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following instance.Do not breaking away under the above-mentioned technological thought situation of the present invention, various replacements or change according to ordinary skill knowledge and customary means are made all should comprise within the scope of the invention.
The specific embodiment
Embodiment 1
Choose high-quality ferfas raw material, after cold water or warm water cleaning (reducing the loss of nutriment in the ferfas), evenly sabot is dried in baking oven to moisture 8 (w) % in 50 ℃.Pulverize, collect 5 orders～20 purpose dried particles, pack, sterilization as product.
Embodiment 2
Choose high-quality ferfas raw material, after cleaning by above-mentioned the same manner, evenly sabot, in 40 ℃ in baking oven, dry to moisture be 9 (w) %.Pulverize, collect 16 orders～30 purpose dried particles, pack, sterilization as product.
The ferfas tea of instance 1 after 30 minutes, is collected soak with the boiling water immersion, and surplus tea repeats to soak twice again, at last whole soaks is dissolved the concentration to 0.1g/ml surely, is test specimen, carries out the experiment of following pharmacodynamics and function assessment.
One, ferfas tea of the present invention is to the influence of chicken red blood cell induced mice hemolytic antibody generation
Experimental animal: 40 of KM kind mouse, body weight 20 ± 2g.
Test method: mouse is divided into normal group (distilled water 0.2ml/10g), model group (distilled water 0.2ml/10g) and ferfas tea group of the present invention (30g/kg) at random, every group each 10, male and female half and half.Each organizes the every day of gastric infusion according to dosage, every day 1 time, successive administration 5 days.Behind the 5th day administration 0.5h, with 0.2ml/ only to mouse peritoneal injection chicken red blood cell solution, intraperitoneal injection of cyclophosphamide normal saline solution 20mg/kg simultaneously.Afterwards, respectively the 7th day, the 9th day to mouse peritoneal injection endoxan normal saline solution 20mg/kg, during continue medication.After testing the 11st day mouse stomach 0.5h, get blood 0.025ml with sharp mouth suction pipe, add and fill in the test tube of 1ml physiological saline at mouse eye rear vein beard; Add 4% chicken red blood cell solution 0.5ml afterwards, 15% GPS 0.5ml places 1h in 37 ℃ of water-baths; Ice bath 10min cessation reaction behind the 1h, centrifugal 10 minutes of 2000r/min gets supernatant 1ml; Add in the 3ml hemoglobin application liquid colorimetric in the 540nm place.The result representes with X scholar S, row t check between group.
Result of the test is as shown in table 1: show that through one-way analysis of variance compare with model group, ferfas tea group and model group be the tool significant difference relatively, shows that it causes immunity of organism that significant antagonism (P＜0.05) is lowly arranged to endoxan.This results suggest, ferfas tea group can improve the level that immunocompromised model mice hemolytic antibody generates.
Two, ferfas tea influence that carbon granule in the mouse body is cleaned up
Experimental animal: 40 of KM kind mouse, body weight 20 ± 2g.
Test method: mouse is divided into normal group (distilled water 0.2ml/10g), model group (distilled water 0.2ml/10g) at random
The influence that table 1 ferfas tea generates chicken red blood cell induced mice hemolytic antibody
Annotate: with compared with normal:
*P＜0.01; Compare with model control group:
△P＜0.05
With ferfas tea group of the present invention (30g/kg), every group each 10, male and female half and half.Each organizes the every day of gastric infusion according to dosage, every day 1 time, successive administration 7 days.Except that blank control group, all the other are respectively organized the 7th day beginning every day with 25mg/kg dosage at mouse back hypodermic injection 50% hydrocortisone solution, and blank control group is with isometric(al) normal saline solution hypodermic injection same area, and modeling is 7 days continuously, during continue medication.Test period totally 13 days made an experiment behind the administration 30min in the 14th day.By india ink (the being diluted to 25% india ink) 0.1ml/10g of tail vein injection dilution with gelatin, get blood 0.025ml at mouse eye rear vein beard with sharp mouth suction pipe respectively in injection back 30s and 6min, adding fills 0.1% (1mg/ml) Na
2CO
3In the test tube of 2ml solution, shake up the back in 675nm place colorimetric estimation OD value (OD675).The result representes with X scholar S, row t check between group.
Result of the test is as shown in table 2: show that through one-way analysis of variance compare with model group, ferfas tea group and model group be the tool significant difference relatively, shows that the body's immunity that itself and hydrocortisone cause lowly has significant antagonism (P＜0.05).This results suggest, ferfas tea group have the effect of the phagocytic function that improves mouse body reticuloendothelial system.
The influence that table 2 ferfas tea is cleaned up carbon granule in the chicken red blood cell induced mice body
Annotate: with compared with normal:
*P＜0.05,
*P＜0.01; Compare with model control group:
△P＜0.05
Three, ferfas tea is to 2, the influence of 4-dinitrofluorobenzene inducing mouse ear swelling
Experimental animal: 40 of KM kind mouse, body weight 20 ± 2g.
Test method: mouse is divided into normal group (distilled water 0.2ml/10g), model group (distilled water 0.2ml/10g) and ferfas tea group of the present invention (30g/kg) at random, every group each 10, male and female half and half.The preparatory administration of normal group mouse 3 days, mouse web portion unhairing position was with 1%DNFB (2, a 4-dinitrofluorobenzene) acetone soln 0.025ml/ sensitization in the 4th day; The next day, strengthen once with the same position of method; After sensitization the 5th day, be coated with 1%DNFB (2, the 4-dinitrofluorobenzene) acetone/edible oil (3: 7) solution 0.01ml/ with mouse right ear and only attack; 24h puts to death mouse, cuts ear and takes by weighing left and right sides ear in electronic balance.Model group and ferfas tea group are the same, and at the 4th, 6,8 day lumbar injection CY (100mg/kg).With the difference (mg) of left ear and auris dextra and swelling degree as mouse DTH reaction intensity.
Swelling degree computing formula: (auris dextra weight-left ear is heavy)/mouse body weight.
Result of the test is as shown in table 3: show that through one-way analysis of variance compare with model group, ferfas tea group and model group be the tool significant difference relatively, shows that its immunity of organisms that endoxan is caused lowly has significant antagonism (P＜0.05).The result shows that ferfas tea group can effectively improve the delayed allergy of mouse.
Table 3 ferfas tea is to the influence of Mice Auricle DTH
Annotate: with compared with normal:
*P＜0.05,
*P＜0.01; Compare with model control group:
△P＜0.05
Four, ferfas tea is to the influence of NK cytoactive
Experimental animal: 40 of KM kind mouse, body weight 20 ± 2g.
Test method: mouse is divided into normal group (distilled water 0.2ml/10g), model group (distilled water 0.2ml/10g) and ferfas tea group of the present invention (30g/kg) at random, every group each 10, male and female half and half.Normal group is irritated stomach physiological saline every day, and model group is intraperitoneal injection of cyclophosphamide (50mg/kg) 0.5ml every other day, administration every day of administration group and intraperitoneal injection of cyclophosphamide (50mg/kg) 0.5ml every other day.Experimental period is altogether 9 days, experiment sacrificed by decapitation mouse on the same day.The aseptic spleen of getting places aseptic plate when filling Hanks liquid, gently grinds with the syringe needle stamen, and 200 order nylon net filters are collected and filtrated in test tube 1500min
-1* 5min is centrifugal, abandons supernatant.Every pipe adds the Tris-NH of 3ml pH7.2
4Cl solution lysed erythrocyte, centrifugal, Hanks liquid is washed 2 times, fully cell culture fluid adjustment cell concentration to 1 * 10
7/ ml.With the YAC-1 cell is target cell, establishes NK test hole, natural release aperture, maximum release aperture.Application of sample is done 3 multiple holes for every kind in 96 porocyte culture plates.On ELIASA, detect the OD value with the 490nm wavelength, measure with the LDH method for releasing.Computing formula:
Result of the test is as shown in table 4: show that through one-way analysis of variance compare with model group, ferfas tea group and model group be the tool significant difference relatively, shows that its immunity of organisms that endoxan is caused lowly has significant antagonism (P＜0.05).The result shows that ferfas tea group can effectively improve the NK cytoactive of mouse.
Table 4 ferfas tea is to the influence of NK cytoactive
Annotate: with compared with normal:
*P＜0.01; Compare with model control group:
△P＜0.05
Five, toxicity is observed
1, all can not cause death to mouse filling maximum administration concentration of food and dosage, can't measure the median lethal dose of ferfas tea group.
2, carried out rat teratogenic test, Salmonella reversion test, mouse bone marrow cells micronucleus test and chromosomal aberration test with ferfas tea group of the present invention, proved that this medicine does not have teratogenesis and mutagenesis.
Above-mentioned experimental result shows that the ferfas tea product that adopts the inventive method to make can have the effect and the function of obvious raising immunity of organisms.Ferfas tea product of the present invention, particularly it does not contain the characteristics of the pure natural bacterium tea-drinking article of any adding ingredient and auxiliary material, is utilization of ferfas and giving full play to of function effective components effect thereof, and a kind of more convenient, simple and direct approach is provided.
Claims (4)
1. the preparation method of ferfas tea is characterized in that the ferfas of cleaning is dried to moisture weight content≤10% under≤60 ℃ of temperature conditions, and after the pulverizing, the dried particles of getting particle diameter and be 5～30 mesh sieve scopes is as product.
2. the preparation method of ferfas tea as claimed in claim 1 is characterized in that said bake out temperature is 40 ℃～50 ℃.
3. according to claim 1 or claim 2 the preparation method of ferfas tea is characterized in that said moisture weight content≤9%.
4. adopt the ferfas tea product of the method making of one of claim 1 to 3.
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Cited By (1)
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| CN103719434A (en) * | 2013-12-26 | 2014-04-16 | 攀枝花彝人块菌有限公司 | Truffle tea and preparation method thereof |
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| CN101313920A (en) * | 2008-06-25 | 2008-12-03 | 曹晋忠 | Application of truffle extract in preparing foods, medicine for treating endocrine dyscrasia diseases |
| CN101732362A (en) * | 2008-11-21 | 2010-06-16 | 宁月宝 | Application of wild truffle in preparing medicine with anticancer and immunological functions |
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2010
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Patent Citations (4)
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| CN1742599A (en) * | 2005-09-29 | 2006-03-08 | 广东省微生物研究所 | Hericium erinaceus bag-tea preparing method |
| CN101077989A (en) * | 2007-06-19 | 2007-11-28 | 西北农林科技大学 | Supercritical extraction method of tuber indicum oil |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103719434A (en) * | 2013-12-26 | 2014-04-16 | 攀枝花彝人块菌有限公司 | Truffle tea and preparation method thereof |
| CN103719434B (en) * | 2013-12-26 | 2015-10-14 | 攀枝花彝人块菌有限公司 | A kind of truffle tea and preparation method thereof |
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Application publication date: 20120208 |