CN102335242A - Chinese medicinal oral solution for treating poultry colibacillosis and preparation method thereof - Google Patents
Chinese medicinal oral solution for treating poultry colibacillosis and preparation method thereof Download PDFInfo
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- CN102335242A CN102335242A CN2011103401397A CN201110340139A CN102335242A CN 102335242 A CN102335242 A CN 102335242A CN 2011103401397 A CN2011103401397 A CN 2011103401397A CN 201110340139 A CN201110340139 A CN 201110340139A CN 102335242 A CN102335242 A CN 102335242A
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Abstract
The invention provides a Chinese medicinal oral solution for treating poultry colibacillosis and a preparation method thereof. The Chinese medicinal oral solution consists of the following components in parts by weight: 20 to 80 parts of common andrographis herb, 10 to 60 parts of red peony root, 10 to 60 parts of indigowoad leaf, 10 to 60 parts of humifuse euphorbia herb and 20 to 60 parts of Chinese rhubarb. The preparation method comprises the following steps: weighing the common andrographis herb and the Chinese rhubarb according to the parts by weight with ethanol to leak; and extracting the red peony root, the indigowoad leaf and the humifuse euphorbia herb with water, and mixing to prepare the oral solution. The Chinese medicinal oral solution for treating poultry colibacillosis has a reasonable prescription and a remarkable effect, can be used for solving the problem of inconvenience in use of Chinese medicinal herbs, has an effective prevention and treatment effect on poultry drug-resistance colibacillosis infection which regularly occurs clinically, and has a remarkable effect on colibacillus inflammation reaction and sepsis caused by endotoxin; and the preparation method is simple, and is suitable for the requirement of industrial large-scale production.
Description
Technical field
The present invention relates to a kind of Chinese medicine oral liquid and preparation method thereof, especially a kind ofly be used to treat sick Chinese medicine oral liquid of poultry colibacillosis and preparation method thereof.
Background technology
Avian colibacillosis is the bacterial infectious disease that avian escherichia coli causes; Each stage in the poultry growth all can take place; Its high-incidence season is chick stage and laying hen egg-laying peak, and clinical manifestation is diseases such as pericarditis, airsacculitis, perihepatitis, peritonitis, salpingitis, omphalitis, ophthalmia, escherichia coli granuloma.Common avian escherichia coli pathogenic strain comprises O
1, O
2, O
35, O
78, sickness rate 11%～69%, mortality rate 3.8%～72.9%, fatality rate is 40.2%～90.3%, has become one of topmost infectious disease clinically.
Treat colibacillosis clinically and mainly use antibiotic; But because the influence of antibiotic general usefulness and abuse, poultry farming ecological deterioration, breeding way change and the low inferior factor of technical merit; Multidrug resistant escherichia coli have appearred in present stage, have had a strong impact on the clinical therapeutic efficacy of poultry colibacillosis, cause colibacillosis frequently to break out; Bring serious threat to aviculture, even caused enormous economic loss.Meanwhile, drug resistance also is to cause colibacillosis to become the one of the main reasons of frequently-occurring disease, and the colibacillary drug resistance problem of China poultry is serious and complicated at present; Almost all antibacterials have all been produced drug resistance, the raiser is using antibiotic as the colibacillary main means of control, in practical application; Because the drug sensitive test popularity rate is low, medication blindness is big, arbitrarily escalated dose; The low dosage life-time service is not noted medication by turns, causes the escherichia coli drug resistance serious.China Veterinery Drug Inspection Office studies the back to the bacterial strain of preserving and finds that the 1950's, there was not drug resistance basically in strain separated; The resistant strains spectrum of the sixties is also narrower, is merely 20%; The seventies, colibacillary drug resistance increases sharply; The eighties, mostly strain separated was multiple antibiotic resistant strain; The nineties, isolated strains had 87% anti-antibiotics more than 5 kinds simultaneously, and had detected 8 anti-, 9 anti-super Resistant strains at present clinically, and is visible, chemical sproofly caused drug effect to descend even invalid, and medicine control difficulty increases.
Endotoxin in the fowl Escherichia coli is one of important paathogenic factor of chicken colibacillosis; It is the main matter that causes systemic inflammatory reaction or pyemia; And antibiotic (except that polymyxin) does not have effect to endotoxin in vivo; And the antibiotic use of part also can cause endotoxic release, quickened endotoxin accumulating in vivo, and then caused the inflammatory reaction and the septicemia of part or whole body.Therefore; Utilize the natural resources of Chinese medicinal materials of China's abundant; Through reasonable formula to Chinese crude drug, develop the Chinese medicine preparation that can effectively improve the colibacillosis cure rate, when the minimizing escherichia coli are to aquaculture harm, reduce antibiotic use or abuse; For people provide the animal products that has no side effect, have huge market potential and far-reaching social meaning.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis.
Another technical problem to be solved by this invention is to provide the above-mentioned method for preparing that is used to treat the Chinese medicine oral liquid of poultry colibacillosis.
For solving the problems of the technologies described above, technical scheme of the present invention is:
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis; Form by Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, by 20～80 parts of its parts by weight Herba Andrographis, 10～60 parts of Radix Paeoniae Rubra, 10～60 parts of Folium Isatidiss, 10～60 parts of Herba Euphorbiae Humifusaes, 20～60 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 40 parts of its parts by weight Herba Andrographis, 20 parts of Radix Paeoniae Rubra, 20 parts of Folium Isatidiss, 20 parts of Herba Euphorbiae Humifusaes, 30 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 60 parts of its parts by weight Herba Andrographis, 30 parts of Radix Paeoniae Rubra, 40 parts of Folium Isatidiss, 30 parts of Herba Euphorbiae Humifusaes, 40 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 80 parts of its parts by weight Herba Andrographis, 60 parts of Radix Paeoniae Rubra, 40 parts of Folium Isatidiss, 40 parts of Herba Euphorbiae Humifusaes, 50 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 20 parts of its parts by weight Herba Andrographis, 10 parts of Radix Paeoniae Rubra, 60 parts of Folium Isatidiss, 60 parts of Herba Euphorbiae Humifusaes, 20 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 50 parts of its parts by weight Herba Andrographis, 50 parts of Radix Paeoniae Rubra, 30 parts of Folium Isatidiss, 50 parts of Herba Euphorbiae Humifusaes, 60 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 30 parts of its parts by weight Herba Andrographis, 40 parts of Radix Paeoniae Rubra, 10 parts of Folium Isatidiss, 40 parts of Herba Euphorbiae Humifusaes, 35 parts of Radix Et Rhizoma Rhei.
Preferably, the above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis is by 70 parts of its parts by weight Herba Andrographis, 45 parts of Radix Paeoniae Rubra, 25 parts of Folium Isatidiss, 10 parts of Herba Euphorbiae Humifusaes, 55 parts of Radix Et Rhizoma Rhei.
The above-mentioned method for preparing that is used to treat the Chinese medicine oral liquid of poultry colibacillosis, concrete steps are:
(1) meter takes by weighing 20～80 parts of Herba Andrographis, 20～60 parts of Radix Et Rhizoma Rhei by weight, with 65% (v/v) ethanol seepage, collects 5 times of solution to Herba Andrographis and Radix Et Rhizoma Rhei gross weight, and concentrating under reduced pressure is subsequent use;
(2) meter takes by weighing 10～60 parts of Herba Euphorbiae Humifusaes, 10～60 parts of Folium Isatidiss, 10～60 parts of Radix Paeoniae Rubra by weight, adds 6～8 times of water to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, warm macerating half an hour; Decoct and extract 2 times, each 2 hours, collecting decoction; High pressure filters, and concentrated filtrate is collected subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 8～10% sodium hydroxide solutions adjusting pH to 5.0～7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Preferably, the above-mentioned method for preparing that is used to treat the Chinese medicine oral liquid of poultry colibacillosis, concrete steps are:
(1) meter takes by weighing 20～80 parts of Herba Andrographis, 20～60 parts of Radix Et Rhizoma Rhei by weight, with 65% (v/v) ethanol seepage, collects 5 times of solution to Herba Andrographis and Radix Et Rhizoma Rhei gross weight, is evaporated to relative density 1.20～1.25, and is subsequent use;
(2) meter takes by weighing 10～60 parts of Herba Euphorbiae Humifusaes, 10～60 parts of Folium Isatidiss, 10～60 parts of Radix Paeoniae Rubra by weight, adds 6～8 times of water to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, warm macerating half an hour; Decoct and extract 2 times; Each 2 hours, collecting decoction, high pressure filters; Concentrated filtrate to relative density 1.20～1.25, subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 8～10% sodium hydroxide solutions adjusting pH to 5.0～7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Above-mentioned relative density is meant the proportion of solution/filtrating.
The Chinese medicine oral liquid that is used to treat poultry colibacillosis that above-mentioned method for preparing is processed when being used to treat poultry colibacillosis, according to sneaking into 1.5ml～2.5ml Chinese medicine oral liquid in every 1L water, is mixed drink, and every day 2 times, 3-5 days is a course of treatment.
The invention has the beneficial effects as follows:
The above-mentioned Chinese medicine oral liquid that is used to treat poultry colibacillosis, reasonable recipe, effect are remarkable, have solved the awkward problem of Chinese herbal medicine; Simultaneously has the effectively preventing effect for clinical normal poultry drug resistance coli-infection; Especially the escherichia coli inflammatory reaction and the septicemia effect that endotoxin are caused are remarkable, and each component is the material of integration of edible and medicinal herbs, have ensured the safety of animal-derived food greatly; Its preparation method is simple, is fit to requirements of large-scale industrial production.
The specific embodiment
Below in conjunction with specific embodiment technical scheme according to the invention is further described.
Embodiment 1
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 40kg, Radix Paeoniae Rubra 20kg, Folium Isatidis 20kg, Herba Euphorbiae Humifusae 20kg, Radix Et Rhizoma Rhei 30kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 40kg, Radix Et Rhizoma Rhei 30kg,, collect 5 times of solution, be evaporated to relative density 1.22 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 20kg, Folium Isatidis 20kg, Radix Paeoniae Rubra 20kg, add 6 times of water, warm macerating half an hour to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight; Decoct and extract 2 times, each 2 hours, collecting decoction; High pressure filters, and concentrated filtrate to relative density 1.23 is collected subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 10% sodium hydroxide solution adjusting pH to 6.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 2
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 60kg, Radix Paeoniae Rubra 30kg, Folium Isatidis 40kg, Herba Euphorbiae Humifusae 30kg, Radix Et Rhizoma Rhei 40kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 60kg, Radix Et Rhizoma Rhei 40kg,, collect 5 times of solution, be evaporated to relative density 1.20 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 30kg, Folium Isatidis 40kg, Radix Paeoniae Rubra 30kg, add 8 times of water, warm macerating half an hour decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, each 2 hours, collecting decoction, high pressure filters, and concentrated filtrate to relative density 1.25 is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 8% sodium hydroxide solution adjusting pH to 7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 3
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 80kg, Radix Paeoniae Rubra 60kg, Folium Isatidis 40kg, Herba Euphorbiae Humifusae 40kg, Radix Et Rhizoma Rhei 50kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 80kg, Radix Et Rhizoma Rhei 50kg,, collect 5 times of solution, be evaporated to relative density 1.25 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 40kg, Folium Isatidis 40kg, Radix Paeoniae Rubra 60kg, add 7 times of water, warm macerating half an hour decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, each 2 hours, collecting decoction, high pressure filters, and concentrated filtrate to relative density 1.20 is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 9% sodium hydroxide solution adjusting pH to 6.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 4
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 20kg, Radix Paeoniae Rubra 10kg, Folium Isatidis 60kg, Herba Euphorbiae Humifusae 60kg, Radix Et Rhizoma Rhei 20kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 20kg, Radix Et Rhizoma Rhei 20kg,, collect 5 times of solution, be evaporated to relative density 1.21 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 60kg, Folium Isatidis 60kg, Radix Paeoniae Rubra 10kg, add 6 times of water, warm macerating half an hour decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, each 2 hours, collecting decoction, high pressure filters, and concentrated filtrate to relative density 1.22 is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 8% sodium hydroxide solution adjusting pH to 5.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 5
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 50kg, Radix Paeoniae Rubra 50kg, Folium Isatidis 30kg, Herba Euphorbiae Humifusae 50kg, Radix Et Rhizoma Rhei 60kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 50kg, Radix Et Rhizoma Rhei 60kg,, collect 5 times of solution, be evaporated to relative density 1.24 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 50kg, Folium Isatidis 30kg, Radix Paeoniae Rubra 50kg, add 8 times of water, warm macerating half an hour decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, each 2 hours, collecting decoction, high pressure filters, and concentrated filtrate to relative density 1.24 is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 10% sodium hydroxide solution adjusting pH to 7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 6
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 30kg, Radix Paeoniae Rubra 40kg, Folium Isatidis 10kg, Herba Euphorbiae Humifusae 40kg, Radix Et Rhizoma Rhei 35kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 30kg, Radix Et Rhizoma Rhei 35kg,, collect 5 times of solution, be evaporated to relative density 1.25 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 40kg, Folium Isatidis 10kg, Radix Paeoniae Rubra 40kg, add 7 times of water, warm macerating half an hour, decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight; Each 2 hours, collecting decoction, high pressure filters; Concentrated filtrate is collected to relative density 1.24, and is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 9% sodium hydroxide solution adjusting pH to 7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Embodiment 7
A kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis is made up of Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, wherein Herba Andrographis 70kg, Radix Paeoniae Rubra 45kg, Folium Isatidis 25kg, Herba Euphorbiae Humifusae 10kg, Radix Et Rhizoma Rhei 55kg, and concrete method for preparing is:
(1) take by weighing Herba Andrographis 70kg, Radix Et Rhizoma Rhei 55kg,, collect 5 times of solution, be evaporated to relative density 1.24 to Herba Andrographis and Radix Et Rhizoma Rhei gross weight with 65% (v/v) ethanol seepage, subsequent use;
(2) take by weighing Herba Euphorbiae Humifusae 10kg, Folium Isatidis 25kg, Radix Paeoniae Rubra 45kg, add 6 times of water, warm macerating half an hour decoct and extract 2 times to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, each 2 hours, collecting decoction, high pressure filters, and concentrated filtrate to relative density 1.23 is subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 10% sodium hydroxide solution adjusting pH to 6.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
Each amounts of components of the said Chinese crude drug of one of embodiment 1-7 is increased or reduces according to same ratio, and the parts by weight relation of each component of gained all belongs to protection scope of the present invention.
The said Chinese medicine of embodiment 1-7 all meets " Chinese veterinary drug allusion quotation " version two ministerial standards in 2010.
The composition principle of Chinese medicine oral liquid according to the invention is: mainly rush down lower class Chinese medicine by heat-clearing and toxic substances removing, blood circulation promoting and blood stasis dispelling, purging FU-organs and form; Can pass through the directly endotoxic structure of destruction, the various cytokines of antagonism, the detoxification ability that strengthens liver, minimizing to the endotoxic absorption of intestinal source property, thereby anti-endotoxin is to the damage of body effectively; From a plurality of link antagonism escherichia coli, especially endotoxic pathogenic effects is is effectively prevented and treated various inflammatory reactions and septicemia that escherichia coli cause.Wherein: Herba Andrographis heat-clearing and toxic substances removing, Radix Paeoniae Rubra cool the blood dissipate blood stasis are monarch altogether; Folium Isatidis and Herba Euphorbiae Humifusae heat-clearing and toxic substances removing, cooling blood for hemostasis, the two all can strengthen monarch drug renders a service, and is ministerial drug altogether; Radix Et Rhizoma Rhei detoxifcation removing heat from blood, removing mass clots absorbing, purging heat to relax the bowels are assistant; All medicines share, Synergistic, and heat-clearing and toxic substances removing removes humidifying and stagnates eliminating pathogenic heat from blood to cure dysentery.
In order to understand essence of the present invention better, existing clinical manifestation of sick chicken being treated with embodiment 1 said Chinese medicine oral liquid is an example, and the superiority of Chinese medicine oral liquid according to the invention is explained further details:
Test Example 1:
1.1 experimental technique and result judge
Supply the preparation of reagent liquid: with distilled water 1g/mL, 0.5g/mL, 0.25g/mL, 0.125g/mL are processed in times dilutions such as embodiment 1 said Chinese medicine oral liquid, it is for use to put refrigerator.
The preparation of bacterium liquid: get an inoculating loop escherichia coli strain from slant medium, be inoculated in the broth medium, cultivated 18 hours at 37 ℃, take out, it is subsequent use to do 1: 500 dilution bacterium liquid to original bacteria liquid.
Plain agar is dull and stereotyped: the by specification preparation.
Oxford agar diffusion method: draw certain density indicator bacteria bacteria suspension 0.4mL to the flat board of plain agar, be coated with evenly with aseptic glass rake, and aseptic Oxford cup put into culture dish gently, in the plate of horizontal positioned, place 4 Oxford cups equably with tweezers; The confession reagent liquid of accurately drawing the variable concentrations of 0.2mL adds respectively in the cup of Oxford, and marked places 37 ℃ to cultivate 18 hours; Measure antibacterial circle diameter with slide gauge, record analysis; The medicinal liquid of each concentration carries out 3 times to be repeated; The result sees table 1.
Criterion as a result: antibacterial circle diameter (D) is quick for extremely as d＞20mm, and 15mm＜d＜20mm is Gao Min, and 10mm＜d＜15mm is quick in being, 7mm＜d＜10mm is a hypoallergenic, and 0＜d＜7 are insensitive
1.2 result
Table 1 intestinal is defended the oral liquid antibacterial circle diameter (n=3 of mm x ± SD)
Can find out that from last table the embodiment 1 said Chinese medicine oral liquid of variable concentrations all has certain effect to escherichia coli, wherein extremely responsive to escherichia coli during 1g/mL; 0.5g/mL, extremely sensitive to escherichia coli during 0.25g/mL; 0.125g/mL the time to the escherichia coli medium sensitivity.
Test Example 2:
Choose 100 of the identical AA broiler of the body weight of 20 ages in days, be divided into 5 groups at random, 20 every group.Blank group chicken wing intravenous injection 0.2mL distilled water, experimental group (application implementation example 1 said Chinese medicine oral liquid) and every chicken wing intravenous injection of medicine matched group 0.2mL Escherichia coli bacteria liquid (the highly pathogenic escherichia coli that the laboratory isolation identification is preserved); Inject and carried out corresponding treatment property test (depressed, the minimizing of searching for food of spirit appears in the experimental group chicken) in back 2 hours, the result sees table 2.
Table 2
Experimental result shows, the blank group is all dead after 5 days; Low dose group has 10 recoveries from illness, in 5 rehabilitation courses, and 5 death; Middle dose groups has 16 recoveries from illness, 2 not recoveries from illness, 2 death; High dose group, 19 whole rehabilitations of chicken, 1 death.The medicine matched group, 19 chicken recoveries from illness, 1 death.In therapeutic process, the mental status of experimental group, the recovery of feed intake are fast than the medicine matched group, and weightening finish is significantly higher than the medicine matched group.
Can find out that from experimental result the low dose group cure rate is 50%, effective percentage is 75%; Middle and high dose groups, effective percentage is identical, and middle dose groups cure rate is lower than high dose group; Compare with the medicine matched group; The cure rate of high dose group is identical with it; Reach 95%, explain that intestinal defends the effect zero difference of oral liquid and enrofloxacin, but the recovery of the mental status of medicine matched group, feed intake and broiler growth amount are then apparently higher than the medicine matched group; Analysis-by-synthesis is visible, and high dose group is as the therapeutic dose effect highly significant of early stage coli-infection.
Test Example 3
In JIUYUE, 2010, Linyi Prefecture, Shandong chicken house, 25 ages in days, 3000 white plumage broiler outburst colibacillosiss; Dead about 30 of every day mainly shows as the spiritual depressed of full crowd, the minimizing of searching for food, in addition useless exhausted; The part chicken is slow-witted upright, and two wings are sagging, and the yellow-white loose stool is drawn in the necking down of closing one's eyes; Glutinous greasy stench is bonded at around the anus, the chicken that has even paralysis and some slight nervous symptoms have occurred.It is visible to cut open inspection, and the splanchnocoel airbag wall is muddy, thickens, and has the cheesy exudate of yellow-white to adhere to; Pericardium thickens, and is attached with a large amount of exudates, pericardium and thoracic cavity adhesion; Hepatomegaly, surface have faint yellow fibrin film to adhere to; Many fibrinous exudates are arranged in the thoracic cavity, adhesion of mesentery, intestinal mucosa is hemorrhage, congested, edema.
Laboratory diagnosis: the aseptic disease pigeon chest chamber and intraperitoneal exudate and liver of taking is inoculated in the broth medium, and behind 37 ℃ of cultivation 18～24h, setting-out separation and Culture on the Mai Kangkai culture medium obtains red bacterium colony, and further pure culture is identified.
According to clinical symptoms, cut open inspection and change and the lab testing result, be diagnosed as chicken colibacillosis at last.
Treatment:, mix drink, every day 2 times, logotype 5 days according to sneaking into 2.0ml embodiment 1 said Chinese medicine oral liquid in every 1L water.After the medication 5 days, the chicken crowd mental status is recovered, and feed intake is normal, anosis chicken death.
Test Example 4:
In March, 2011, the airsacculitis that outburst escherichia coli in area, Kangping, Liaoning cause, wherein 3000 the 20 white plumage broiler of age in days are ill, and sick chicken mainly shows as dyspnea, row's green or yellow green loose stool, dead 40 of every day.Cut open the visible air bag of inspection and thicken muddiness, the fibroid exudate of volume occurs having on pericarditis, the liver, get pericardial fluid and carry out the antibacterial isolation identification, confirm as septicemia and inflammatory reaction that escherichia coli cause.
White plumage broiler is divided into two groups and treats; First group is used ofloxacin soluble powder (Tianjin City Vigor Bioengineering Co., Ltd.'s production); Second group is used the ofloxacin soluble powder to cooperate embodiment 1 said Chinese medicine oral liquid (every 1L water is sneaked into the 2.0ml Chinese medicine oral liquid) to use, used continuously 4 days.
Through treatment, first group dead 52, search for food, drink water, aspect such as spirit all recovers normal; Second group is not had death, and survival rate is 100%, and other all recover normal.
Performance analysis shows that second group therapeutic effect is significantly higher than first group, and visible, Chinese medicine oral liquid according to the invention and antibiotic are united use can effectively control and treat the septicemia that escherichia coli cause.
Above-mentioned detailed description of this a kind of Chinese medicine oral liquid that is used to treat poultry colibacillosis and preparation method thereof being carried out with reference to embodiment; Be illustrative rather than determinate; Can enumerate out several embodiment according to institute's limited range; Therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (10)
1. Chinese medicine oral liquid that is used to treat poultry colibacillosis; It is characterized in that: form by Herba Andrographis, Radix Paeoniae Rubra, Folium Isatidis, Herba Euphorbiae Humifusae and Radix Et Rhizoma Rhei, by 20～80 parts of its parts by weight Herba Andrographis, 10～60 parts of Radix Paeoniae Rubra, 10～60 parts of Folium Isatidiss, 10～60 parts of Herba Euphorbiae Humifusaes, 20～60 parts of Radix Et Rhizoma Rhei.
2. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 40 parts of its parts by weight Herba Andrographis, 20 parts of Radix Paeoniae Rubra, 20 parts of Folium Isatidiss, 20 parts of Herba Euphorbiae Humifusaes, 30 parts of Radix Et Rhizoma Rhei.
3. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 60 parts of its parts by weight Herba Andrographis, 30 parts of Radix Paeoniae Rubra, 40 parts of Folium Isatidiss, 30 parts of Herba Euphorbiae Humifusaes, 40 parts of Radix Et Rhizoma Rhei.
4. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 80 parts of its parts by weight Herba Andrographis, 60 parts of Radix Paeoniae Rubra, 40 parts of Folium Isatidiss, 40 parts of Herba Euphorbiae Humifusaes, 50 parts of Radix Et Rhizoma Rhei.
5. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 20 parts of its parts by weight Herba Andrographis, 10 parts of Radix Paeoniae Rubra, 60 parts of Folium Isatidiss, 60 parts of Herba Euphorbiae Humifusaes, 20 parts of Radix Et Rhizoma Rhei.
6. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 50 parts of its parts by weight Herba Andrographis, 50 parts of Radix Paeoniae Rubra, 30 parts of Folium Isatidiss, 50 parts of Herba Euphorbiae Humifusaes, 60 parts of Radix Et Rhizoma Rhei.
7. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 30 parts of its parts by weight Herba Andrographis, 40 parts of Radix Paeoniae Rubra, 10 parts of Folium Isatidiss, 40 parts of Herba Euphorbiae Humifusaes, 35 parts of Radix Et Rhizoma Rhei.
8. the Chinese medicine oral liquid that is used to treat poultry colibacillosis according to claim 1 is characterized in that: by 70 parts of its parts by weight Herba Andrographis, 45 parts of Radix Paeoniae Rubra, 25 parts of Folium Isatidiss, 10 parts of Herba Euphorbiae Humifusaes, 55 parts of Radix Et Rhizoma Rhei.
9. the described method for preparing that is used to treat the Chinese medicine oral liquid of poultry colibacillosis of one of claim 1-8, it is characterized in that: concrete steps are:
(1) meter takes by weighing 20～80 parts of Herba Andrographis, 20～60 parts of Radix Et Rhizoma Rhei by weight, with 65% (v/v) ethanol seepage, collects 5 times of solution to Herba Andrographis and Radix Et Rhizoma Rhei gross weight, and concentrating under reduced pressure is subsequent use;
(2) meter takes by weighing 10～60 parts of Herba Euphorbiae Humifusaes, 10～60 parts of Folium Isatidiss, 10～60 parts of Radix Paeoniae Rubra by weight, adds 6～8 times of water to Herba Euphorbiae Humifusae, Folium Isatidis and Radix Paeoniae Rubra gross weight, warm macerating half an hour; Decoct and extract 2 times, each 2 hours, collecting decoction; High pressure filters, and concentrated filtrate is collected subsequent use;
(3) stock solution in step (1) and the step (2) is mixed, with 8～10% sodium hydroxide solutions adjusting pH to 5.0～7.0, supplementing water to specification is that to contain the medical material gross weight in every milliliter of oral liquid be 2g.
10. the method for preparing that is used to treat the Chinese medicine oral liquid of poultry colibacillosis according to claim 9, it is characterized in that: the solution decompression of collecting in the said step (1) is concentrated into relative density 1.20～1.25; The filterable decocting liquid of step (2) mesohigh is concentrated into relative density 1.20～1.25.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104147165A (en) * | 2014-08-13 | 2014-11-19 | 韦旭斌 | Traditional Chinese medicine for control of animal Escherichia coli disease and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104147165A (en) * | 2014-08-13 | 2014-11-19 | 韦旭斌 | Traditional Chinese medicine for control of animal Escherichia coli disease and preparation method thereof |
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Application publication date: 20120201 |