CN102329768A - Flora construction method for residual oil gasification exploitation of oil deposit - Google Patents

Flora construction method for residual oil gasification exploitation of oil deposit Download PDF

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CN102329768A
CN102329768A CN201110278644A CN201110278644A CN102329768A CN 102329768 A CN102329768 A CN 102329768A CN 201110278644 A CN201110278644 A CN 201110278644A CN 201110278644 A CN201110278644 A CN 201110278644A CN 102329768 A CN102329768 A CN 102329768A
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flora
oil reservoir
methane
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牟伯中
刘金峰
杨世忠
李凯平
王立影
周蕾
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East China University of Science and Technology
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Abstract

The invention relates to a flora construction method for residual oil gasification exploitation of an oil deposit. The method comprises the following steps of: 1, acquiring an output liquid sample of a producing well of the oil deposit or an active sludge sample polluted by crude oil; 2, adding a hydrocarbon-containing culture medium in the output liquid sample or active sludge sample for carrying out anaerobic enrichment culture; and 3, transferring an enrichment culture obtained in the step 2 into the same hydrocarbon-containing culture medium for carrying out anaerobic culture until the methane is generated. Compared with the prior art, the method can be used for fully utilizing microorganisms in the oil deposit and obtaining the flora which can be used for producing methane through anaerobically degrading hydrocarbon and adapt to the oil deposit environment by directional enrichment, overcomes the deviation and the one-sidedness of the traditional method for manually combining the flora, and lays a foundation for the residual oil gasification development of the oil deposit.

Description

The flora construction process that is used for oil reservoir irreducible oil gasification exploitation
Technical field
The present invention relates to a kind of construction process of anaerobic degradation flora, especially relate to the flora and the construction process thereof that are used for oil reservoir irreducible oil gasification exploitation.
Background technology
Improve China's crude oil RF and have strategic importance.Oil is a national strategy property resource, and over nearly 10 years, the crude oil in China consumption increases with average annual 5.8% speed, and the same period, domestic oil supply rate of growth was merely 1.7%.After China in 1993 became crude oil net importer, the crude oil import amount increased year by year.It is predicted that to the year two thousand twenty, the year import demand of China's oil will be above 500,000,000 tons, externally interdependency will reach 70%, and country will face the great economy safety-problems.Make great efforts to improve the oil-field development level, promote oilfield stable production to alleviate one of effective measure of China's energy shortage situation beyond doubt.Yet most oil fields that present China has developed have got into high moisture development phase middle and later periods, and waterflood recovery efficiency factor is not high at present, and substantial oil is also stayed underground, even adopt tertiary oil recovery technology, the prediction ultimate recovery factor will be less than 50%.The most oil fields of China are faced with a difficult problem and immense pressures such as the exploitation difficulty is big, cost is high, RF is low simultaneously.
The methanogenic phenomenon of petroleum hydrocarbon anaerobic degradation that extensively exists in the environment is for realizing that hydrocarbonaceous habitat biogas melts the possibility of adopting through engineering approaches foundation is provided.The experimental study that the saturated alkane anaerobic degradation produces methane shows that the chemical transformation that laboratory crude oil anaerobic degradation product methane process PetroChina Company Limited. hydrocarbon takes place is consistent with the pattern that changes in the degraded oil reservoir.There is a large amount of evidences to prove that biological methane produces from the degraded oil reservoir.Methane and heavy oil are considered to produced simultaneously, all derive from alkane degradation and produce the methane process.The methane gas isotropic substance has shown that gentlier biogenic methane wants big, the heavier CO of ratio of the methane of the specific heat origin cause of formation in the oil reservoir 2Gas isotope shows that biogenic methane derives from carbon dioxide reduction.Crude oil in the oil reservoir under producing methane conditions this facts explain of degraded the one-tenth merotype of hydrocarbon and the gathering of dry gas in the global degraded oil reservoir.
To the unique anaerobic environment of subsurface deposit; Utilize action of microorganisms; The irreducible oil that conventional reoovery method in the oil reservoir is difficult to employ Degradation and Transformation on the spot is Sweet natural gas (methane), exploits out with the form of Sweet natural gas again, perhaps lays on the spot as strategic resource; Thereby increase substantially the utilising efficiency and the mining level of hydrocarbon resources, further prolong the reservoir development life-span.Mikrobe in the oil reservoir is converted into methane with crude oil and in theory and practice, all has feasibility in anaerobic environment.The degraded of petroleum hydrocarbon anaerobion is an important directions of studying at present, oil reservoir irreducible oil biogas is melted to adopt have important significance for theories and using value.
Crude oil gasification exploitation is regarded as huge bio-reactor with oil reservoir exactly, and the hydrocarbon anaerobic degradation produces the methane process need by difference in functionality flora fellowship, and the performance synergy could be accomplished.This synergy mainly can be divided into two stages, and the one, degradation period, promptly alkane degradation is a small organic molecule; The 2nd, in the aerogenesis stage, promptly small-molecule substance finally changes into methane.The degraded product of fs must constantly be removed by the mikrobe of subordinate phase, and reaction could continue to carry out.Generally speaking, there is rich and varied methanogen in the hydrocarbonaceous habitat.Wherein, mainly the methanogen of hydrogen nutritional type in the high-temperature oil reservoir, acetate need be oxidized to hydrogen and carbonic acid gas through symbiosis acetate and produce methane through the methanogen of hydrogen nutritional type again.This viewpoint is consistent with the result of thermodynamic prediction, i.e. symbiosis acetate oxidation thermodynamics under high-temperature condition is more feasible.In some low temperature environment, the methanogen of acetate nutritional type also occupies certain ratio, shows that the methanogen of acetate nutritional type is also very important at the end of degraded.Yet at other low temperature environment, the methanogen of hydrogen nutritional type still accounts for leading.This shows that the key of this process is the flora that how to make up the hydrocarbon of can under anaerobic environment, degrading.Yet, at present still not about how setting up flora and the construction process thereof that is used for oil reservoir irreducible oil gasification exploitation.
Summary of the invention
The object of the invention is exactly for the defective that overcomes above-mentioned prior art existence a kind of flora construction process that is used for oil reservoir irreducible oil gasification exploitation of enough building degraded hydrocarbon generation methane under the anaerobic conditions in to be provided.
The object of the invention can be realized through following technical scheme:
Be used for the flora construction process of oil reservoir irreducible oil gasification exploitation, it is characterized in that, this method comprises the steps: that (1) gather oil reservoir fluid produced by oil production well sample or by the active sludge sample of crude oil pollution; (2) in said output liquid sample or active sludge sample, add hydrocarbonaceous substratum anaerobism enrichment culture; (3) the gained enrichment culture thing anaerobism of transferring in the identical hydrocarbonaceous substratum is cultivated up to producing methane.
The described hydrocarbonaceous substratum of step (2) is by mixed alkanes 30 μ L～100 μ L, NaCl 0.50g/L, MgCl 26H 2O 0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L and vitamin solution 0.2～2mL and trace element solution 0.1～1mL.
Described mixed alkanes is that Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are by the mixed uniformly alkane of equal-volume.
The composition of described vitamin solution comprises: cobalamin 1.0mg/L, vitamin H 20.0mg/L, folic acid 20.0mg/L; Niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L.
The composition of described trace element solution comprises: CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O 0.10g/L, ZnCl 26H 2O 0.50g/L.
Hydrocarbonaceous substratum and culturing bottle remaining space deoxygenation fully during enrichment culture described in the step (2).
Compared with prior art; The inventive method can make full use of the mikrobe in the oil reservoir; Obtain to adapt to the anaerobic degradation hydrocarbon methanogen floras of reservoir media condition through orienting enriching; Overcome the deviation property and the one-sidedness of traditional artificial combination flora method, for the gasification exploitation of oil reservoir irreducible oil lays the foundation, the constructed flora hydrocarbon generation methane of can under anaerobic conditions, degrading.
Embodiment
Below in conjunction with specific embodiment the present invention is elaborated.
Embodiment 1:
Sample source is in the North China Oilfield produced liquid in oil well, 38 ℃ of its reservoir temperatures.By mixed alkanes 30 μ L (Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are pressed the equal-volume uniform mixing), vitamin solution 0.2mL (cobalamin 1.0mg/L, vitamin H 20.0mg/L, folic acid 20.0mg/L; Niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L) and trace element solution 0.1mL (CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O 0.10g/L, ZnCl 26H 2O 0.50g/L) and inorganic salt solution 45mL (NaCl0.50g/L, MgCl 26H 2O 0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L) forms substratum, be encased in the 120mL serum bottle, sterilization back inoculation produced liquid in oil well 4.5mL, 38 ℃ of cultivations.Through having produced a large amount of methane after the anaerobism cultivation in 1 year, the output increase of back methane is cultivated in switching to this produced liquid in oil well in being inoculated into product methane-rich substratum, and through 356 days anaerobism cultivation, the 1L substratum produced 1500 μ L methane.Bacterium fungus strain formation sees Table 1 in the methanogen floras, and ancient bacterium formation sees Table 2.
Embodiment 2
Sample source is in the Xinjiang Oilfield produced liquid in oil well, 32 ℃ of its reservoir temperatures.By mixed alkanes 70 μ L (Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are pressed the equal-volume uniform mixing), vitamin solution 0.2mL (cobalamin 1.0mg/L, vitamin H 20.0mg/L, folic acid 20.0mg/L; Niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L) and trace element solution 0.1mL (CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O 0.10g/L, ZnCl 26H 2O 0.50g/L) and inorganic salt solution 45mL (NaCl0.50g/L, MgCl 26H 2O 0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L) forms substratum, be encased in the 120mL serum bottle, sterilization back inoculation produced liquid in oil well 4.5mL, 32 ℃ of cultivations.This produced liquid in oil well has produced a large amount of methane after the process anaerobism is cultivated in being inoculated into product methane-rich substratum, the output increase of transferring and cultivating the back methane for 2 times, and through 215 days anaerobism cultivation, the 1L substratum produced 1130 μ L methane.Bacterium fungus strain formation sees Table 1 in the methanogen floras, and ancient bacterium formation sees Table 2.
Embodiment 3
Sample source is in the Shengli Oil Field produced liquid in oil well, 60 ℃ of its reservoir temperatures.By mixed alkanes 100 μ L (Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are pressed the equal-volume uniform mixing), vitamin solution 0.2mL (cobalamin 1.0mg/L, vitamin H 20.0mg/L, folic acid 20.0mg/L; Niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L) and trace element solution 0.1mL (CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O 0.10g/L, ZnCl 26H 2O 0.50g/L) and inorganic salt solution 45mL (NaCl0.50g/L, MgCl 26H 2O 0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L) forms substratum, be encased in the 120mL serum bottle, sterilization back inoculation produced liquid in oil well 4.5mL, 60 ℃ of cultivations.This produced liquid in oil well has produced a large amount of methane after the process anaerobism is cultivated in being inoculated into product methane-rich substratum, the output increase of transferring and cultivating the back methane for 2 times, and through 232 days anaerobism cultivation, the 1L substratum produced 1440 μ L methane.Bacterium fungus strain formation sees Table 1 in the methanogen floras, and ancient bacterium formation sees Table 2.
Embodiment 4
Sample source is sewage from oil refinery jar active sludge in Shanghai.By mixed alkanes 50 μ L (Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are pressed the equal-volume uniform mixing), vitamin solution 0.2mL (cobalamin 1.0mg/L, vitamin H 20.0mg/L, folic acid 20.0mg/L; Niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L) and trace element solution 0.1mL (CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O0.10g/L, ZnCl 26H 2O 0.50g/L) and inorganic salt solution 45mL (NaCl0.50g/L, MgCl 26H 2O0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L) forms substratum, be encased in the 120mL serum bottle, sterilization back inoculation produced liquid in oil well 4.5mL, 37 ℃ of cultivations.Active sludge has produced a large amount of methane after the process anaerobism is cultivated in being inoculated into product methane-rich substratum, and the output increase of methane is transferred for the third time and cultivated after 3 switchings are cultivated, and the 1L substratum produces 1080 μ L methane.Bacterium fungus strain formation sees Table 1 in the methanogen floras, and ancient bacterium formation sees Table 2.
Bacterium percentage composition in table 1 embodiment 1～4 methanogen floras
Figure BDA0000092378340000051
Figure BDA0000092378340000061
Table 2 embodiment 1～4 methanogen floras bacterium in middle ancient times percentage composition
Figure BDA0000092378340000062

Claims (6)

1. be used for the flora construction process of oil reservoir irreducible oil gasification exploitation, it is characterized in that, this method comprises the steps: that (1) gather oil reservoir fluid produced by oil production well sample or by the active sludge sample of crude oil pollution; (2) in said output liquid sample or active sludge sample, add hydrocarbonaceous substratum anaerobism enrichment culture; (3) the gained enrichment culture thing anaerobism of transferring in the identical hydrocarbonaceous substratum is cultivated up to producing methane.
2. the flora construction process that is used for oil reservoir irreducible oil gasification exploitation according to claim 1 is characterized in that the described hydrocarbonaceous substratum of step (2) is by mixed alkanes 30 μ L～100 μ L, NaCl 0.50g/L, MgCl 26H 2O 0.10g/L, CaCl 22H 2O 0.10g/L, NH 4Cl 0.25g/L, KH 2PO 40.20g/L, KCl0.30g/L, NaHCO 32.50g/L, Na 2S9H 2O 0.5g/L and vitamin solution 0.2～2mL and trace element solution 0.1～1mL.
3. the flora construction process that is used for oil reservoir irreducible oil gasification exploitation according to claim 2; It is characterized in that described mixed alkanes is that Pentadecane, n-hexadecane, NSC 172782, Octadecane, NSC 77136 and NSC 62789 are by the mixed uniformly alkane of equal-volume.
4. the flora construction process that is used for oil reservoir irreducible oil gasification exploitation according to claim 2 is characterized in that the composition of described vitamin solution comprises: cobalamin 1.0mg/L; Vitamin H 20.0mg/L, folic acid 20.0mg/L, niacin 50.0mg/L; D-Ca-pantothenate 50.0mg/L, p-aminobenzoic acid 50.0mg/L, Y factor-hydrochloric acid 100.0mg/L; Vitamin G 50.0mg/L, VITMAIN B1-hydrochloric acid (2H 2O) 50.0mg/L, Thioctic Acid 50.0mg/L.
5. the flora construction process that is used for oil reservoir irreducible oil gasification exploitation according to claim 2 is characterized in that the composition of described trace element solution comprises: CoCl 26H 2O 0.50g/L, CuCl 22H 2O 0.10g/L, FeCl 24H 2O 7.50g/L, H 3BO 31.00g/L, MnCl 24H 2O 0.50g/L, Na 2MoO 42H 2O 0.10g/L, NiCl 26H 2O 0.10g/L, ZnCl 26H 2O 0.50g/L.
6. the flora construction process that is used for the gasification exploitation of oil reservoir irreducible oil according to claim 1 is characterized in that, hydrocarbonaceous substratum and the deoxygenation fully of culturing bottle remaining space during enrichment culture described in the step (2).
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CN113956860A (en) * 2021-09-16 2022-01-21 华东理工大学 Construction method of microbial corrosion control system of oil field system
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US11578575B2 (en) 2015-09-22 2023-02-14 9668241 Canada Inc. Microbially enhanced thermal oil recovery
CN107152266B (en) * 2016-03-03 2020-05-15 中国石油化工股份有限公司 Method for improving biogasification rate of residual oil in oil reservoir and application of method
CN107152266A (en) * 2016-03-03 2017-09-12 中国石油化工股份有限公司 Improve the method and its application of the biogasification speed of oil reservoir residual oil
CN106929419A (en) * 2017-04-12 2017-07-07 临沂大学 A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras
CN107413191A (en) * 2017-04-12 2017-12-01 临沂大学 A kind of method for catching of organic exhaust gas
CN107201311A (en) * 2017-04-12 2017-09-26 临沂大学 A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras
CN106929420A (en) * 2017-04-12 2017-07-07 临沂大学 A kind of method of decomposing petroleum hydrocarbon
CN110043234A (en) * 2019-04-04 2019-07-23 农业部沼气科学研究所 A kind of oil sediment processing method and activation oil sediment microorganism are come the method that improves oil recovery factor
CN112576229A (en) * 2020-12-11 2021-03-30 大庆油田有限责任公司 Method for producing methane from underground crude oil by utilizing microbial action
CN113956860A (en) * 2021-09-16 2022-01-21 华东理工大学 Construction method of microbial corrosion control system of oil field system
CN114958643A (en) * 2021-11-12 2022-08-30 农业部沼气科学研究所 Efficient enrichment culture method of petroleum hydrocarbon degradation methanogen strain, product and application

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