CN102329005A

CN102329005A - Microbial preparation for aquaculture and preparation method thereof

Info

Publication number: CN102329005A
Application number: CN201110165822A
Authority: CN
Inventors: 谢克焕; 王滨; 王�华
Original assignee: DALIAN GOLD TONE SEAFOOD Co Ltd
Current assignee: DALIAN GOLD TONE SEAFOOD Co Ltd
Priority date: 2011-06-20
Filing date: 2011-06-20
Publication date: 2012-01-25
Anticipated expiration: 2031-06-20
Also published as: CN102329005B

Abstract

The invention provides a microbial preparation for aquaculture and a preparation method thereof. The microbial preparation is a product obtained by fermenting bacillus in a culture medium consisting of glucose, saccharose, corn steep liquor, monopotassium phosphate and seawater. The preparation method comprises the steps of culture medium preparation, high-temperature sterilization and inoculating fermentation. The microbial preparation has the advantages of regulating water quality substrate, improving culture environment, promoting growth, preventing diseases and the like.

Description

A kind of used for aquiculture microbial preparation and preparation method

Technical fieldThe invention belongs to aquaculture field, especially belong to a kind of preparation method of used for aquiculture microbial preparation.

Background technologyAlong with the fast development of culture fishery, particularly greatly develop today of batch production high-density breeding, cultivate good breeding environment, enhance productivity, reducing production costs becomes the essential condition of aquaculture.Objectionable impurities in holothruian cultures field degraded water body; Improve seawater quality and play particularly important effect for the growth of sea cucumber, and the existing microbial preparation kind that is used for used for aquiculture seldom, the microbial preparation that uses for sea-foods such as being fit to sea cucumber is famine especially; Some aquaculture enterprises culture the antibiotics input in the pond at present; Microbiotic only plays sterilization effects, and long-time the use can be reduced water quality, influences the growth of sea cucumber.

Summary of the inventionThe object of the present invention is to provide and a kind ofly regulate the water quality substrate, improve breeding environment, promote growth, preventing disease, low, effective used for aquiculture microbial preparation and the preparation method of cost.

Used for aquiculture microbial preparation and preparation method that the present invention proposes, it is the product that genus bacillus is fermented in the substratum of being made up of glucose, sucrose, steeping water, potassium primary phosphate and seawater and obtains.

Its preparation process is following:

Step 1: the preparation of substratum, the component ratio of substratum is: glucose 1～1.5g, sucrose 2～5g, steeping water 3～5g, potassium primary phosphate 0.02～0.05g, seawater 1000ml, pH value are 4.8～5.5.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, cool the temperature to normal temperature then.

Step 3: inoculation fermentation, in above-mentioned liquid medium, inoculate 1200～1500 ml genus bacillus, regulator solution temperature to 29～31 ℃; PH value is 4～7, stirs 100～120r/min, aerobic culture 36～48h; Grow gemma until thalline, put into fermentor tank then, leavening temperature is 28～34 ℃; Fermentation time is 25～30h, and the pH value scope is 4.8～5.5.

Usage: this preparation directly dropped into to culture in the pond by 1/100000 consumption get final product.

The present invention compared with prior art has following advantage:

1, objectionable impuritiess such as the ammonia nitrogen in the degrading cultivation water, nitrite are improved breeding environment;

2, efficient, fast adaptation sea water culture environment prolongs and changes the water time;

3, preventing disease, the growth of promotion sea cucumber improve output.

Description of drawingsFig. 1 uses preparation of the present invention and the comparison diagram that does not use preparation ammonia nitrogen amount of the present invention to reduce for aquaculture water.

EmbodimentExample 1, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1g, sucrose 2g, steeping water 3g, potassium primary phosphate 0.02g, seawater 1000ml, pH value are 4.8.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110 ℃, keep 20min, cool the temperature to normal temperature then.

Step 3: inoculation fermentation, inoculation 1200 ml genus bacillus in above-mentioned liquid medium, regulator solution temperature to 29 ℃, pH value is 4; Stir 100r/min, aerobic culture 36h grows gemma until thalline, puts into fermentor tank then; Leavening temperature is 28 ℃, and fermentation time is 25h, pH value 4.8.

The preparation that obtains according to above step is fed into 500/jin sea cucumber seedling and cultures in the pond; The ammonia nitrogen quantitative changeization of the aquaculture water in ten days is as shown in Figure 1; The ■ line representes to use the variable quantity of ammonia nitrogen amount of the present invention among Fig. 1; ▲ line is expressed as the variable quantity that does not use ammonia nitrogen amount of the present invention, and according to shown in Figure 1, the present invention has and significantly falls the ammonia nitrogen effect.Except that falling ammonia nitrogen, the present invention also has certain promoter action to the growth of seedling, and shown in following form, this form is for using the present invention and the growth counting rate meter that does not use seedling of the present invention:

?	March 15	March 31	Weightening finish	Rate of increase	The workshop
						The present invention	37	71	34	47.90%	1
The present invention	39	70	31	44.30%	2
						Blank	43	62	19	31%	3
Blank	45	63	18	29%	4

Shown in form, use the present invention can obviously increase the growth of seedling.

Example 2, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1.5g, sucrose 5g, steeping water 5g, potassium primary phosphate 0.05g, seawater 1000ml, pH value are 5.5.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 121 ℃, keep 30min, cool the temperature to normal temperature then.

Step 3: inoculation fermentation, inoculation 1500 ml genus bacillus in above-mentioned liquid medium, regulator solution temperature to 31 ℃, pH value is 4; Stir 120r/min, aerobic culture 48h grows gemma until thalline, puts into fermentor tank then; Leavening temperature is 34 ℃, and fermentation time is 30h, and the pH value scope is 5.5.

Claims

1. used for aquiculture microbial preparation is characterized in that: it is the product that genus bacillus is fermented in the substratum of being made up of glucose, sucrose, steeping water, potassium primary phosphate and seawater and obtains.

2. the preparation method of a used for aquiculture microbial preparation is characterized in that: it is according to following steps preparation, step 1: the preparation of substratum; The component ratio of substratum is: glucose 1～1.5g; Sucrose 2～5g, steeping water 3～5g, potassium primary phosphate 0.02～0.05g; Seawater 1000ml, pH value are 4.8～5.5;

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, cool the temperature to normal temperature then;