CN102329005A - Microbial preparation for aquaculture and preparation method thereof - Google Patents
Microbial preparation for aquaculture and preparation method thereof Download PDFInfo
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- CN102329005A CN102329005A CN201110165822A CN201110165822A CN102329005A CN 102329005 A CN102329005 A CN 102329005A CN 201110165822 A CN201110165822 A CN 201110165822A CN 201110165822 A CN201110165822 A CN 201110165822A CN 102329005 A CN102329005 A CN 102329005A
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- preparation
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- temperature
- fermentor tank
- microbial
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- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 238000009360 aquaculture Methods 0.000 title claims abstract description 14
- 230000000813 microbial effect Effects 0.000 title claims abstract description 12
- 244000144974 aquaculture Species 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 9
- 230000004151 fermentation Effects 0.000 claims abstract description 9
- 239000013535 sea water Substances 0.000 claims abstract description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 7
- 229930006000 Sucrose Natural products 0.000 claims abstract description 7
- 239000008103 glucose Substances 0.000 claims abstract description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 8
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 4
- 241000726221 Gemma Species 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 240000008042 Zea mays Species 0.000 abstract 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 1
- 235000005822 corn Nutrition 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 235000001727 glucose Nutrition 0.000 abstract 1
- 238000011177 media preparation Methods 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 7
- 241000251511 Holothuroidea Species 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
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Abstract
The invention provides a microbial preparation for aquaculture and a preparation method thereof. The microbial preparation is a product obtained by fermenting bacillus in a culture medium consisting of glucose, saccharose, corn steep liquor, monopotassium phosphate and seawater. The preparation method comprises the steps of culture medium preparation, high-temperature sterilization and inoculating fermentation. The microbial preparation has the advantages of regulating water quality substrate, improving culture environment, promoting growth, preventing diseases and the like.
Description
Technical fieldThe invention belongs to aquaculture field, especially belong to a kind of preparation method of used for aquiculture microbial preparation.
Background technologyAlong with the fast development of culture fishery, particularly greatly develop today of batch production high-density breeding, cultivate good breeding environment, enhance productivity, reducing production costs becomes the essential condition of aquaculture.Objectionable impurities in holothruian cultures field degraded water body; Improve seawater quality and play particularly important effect for the growth of sea cucumber, and the existing microbial preparation kind that is used for used for aquiculture seldom, the microbial preparation that uses for sea-foods such as being fit to sea cucumber is famine especially; Some aquaculture enterprises culture the antibiotics input in the pond at present; Microbiotic only plays sterilization effects, and long-time the use can be reduced water quality, influences the growth of sea cucumber.
Summary of the inventionThe object of the present invention is to provide and a kind ofly regulate the water quality substrate, improve breeding environment, promote growth, preventing disease, low, effective used for aquiculture microbial preparation and the preparation method of cost.
Used for aquiculture microbial preparation and preparation method that the present invention proposes, it is the product that genus bacillus is fermented in the substratum of being made up of glucose, sucrose, steeping water, potassium primary phosphate and seawater and obtains.
Its preparation process is following:
Step 1: the preparation of substratum, the component ratio of substratum is: glucose 1～1.5g, sucrose 2～5g, steeping water 3～5g, potassium primary phosphate 0.02～0.05g, seawater 1000ml, pH value are 4.8～5.5.
Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, cool the temperature to normal temperature then.
Step 3: inoculation fermentation, in above-mentioned liquid medium, inoculate 1200～1500 ml genus bacillus, regulator solution temperature to 29～31 ℃; PH value is 4～7, stirs 100～120r/min, aerobic culture 36～48h; Grow gemma until thalline, put into fermentor tank then, leavening temperature is 28～34 ℃; Fermentation time is 25～30h, and the pH value scope is 4.8～5.5.
Usage: this preparation directly dropped into to culture in the pond by 1/100000 consumption get final product.
The present invention compared with prior art has following advantage:
1, objectionable impuritiess such as the ammonia nitrogen in the degrading cultivation water, nitrite are improved breeding environment;
2, efficient, fast adaptation sea water culture environment prolongs and changes the water time;
3, preventing disease, the growth of promotion sea cucumber improve output.
Description of drawingsFig. 1 uses preparation of the present invention and the comparison diagram that does not use preparation ammonia nitrogen amount of the present invention to reduce for aquaculture water.
EmbodimentExample 1, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1g, sucrose 2g, steeping water 3g, potassium primary phosphate 0.02g, seawater 1000ml, pH value are 4.8.
Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110 ℃, keep 20min, cool the temperature to normal temperature then.
Step 3: inoculation fermentation, inoculation 1200 ml genus bacillus in above-mentioned liquid medium, regulator solution temperature to 29 ℃, pH value is 4; Stir 100r/min, aerobic culture 36h grows gemma until thalline, puts into fermentor tank then; Leavening temperature is 28 ℃, and fermentation time is 25h, pH value 4.8.
The preparation that obtains according to above step is fed into 500/jin sea cucumber seedling and cultures in the pond; The ammonia nitrogen quantitative changeization of the aquaculture water in ten days is as shown in Figure 1; The ■ line representes to use the variable quantity of ammonia nitrogen amount of the present invention among Fig. 1; ▲ line is expressed as the variable quantity that does not use ammonia nitrogen amount of the present invention, and according to shown in Figure 1, the present invention has and significantly falls the ammonia nitrogen effect.Except that falling ammonia nitrogen, the present invention also has certain promoter action to the growth of seedling, and shown in following form, this form is for using the present invention and the growth counting rate meter that does not use seedling of the present invention:
| ? | March 15 | March 31 | Weightening finish | Rate of increase | The workshop |
| The present invention | 37 | 71 | 34 | 47.90% | 1 |
| The present invention | 39 | 70 | 31 | 44.30% | 2 |
| Blank | 43 | 62 | 19 | 31% | 3 |
| Blank | 45 | 63 | 18 | 29% | 4 |
Shown in form, use the present invention can obviously increase the growth of seedling.
Example 2, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1.5g, sucrose 5g, steeping water 5g, potassium primary phosphate 0.05g, seawater 1000ml, pH value are 5.5.
Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 121 ℃, keep 30min, cool the temperature to normal temperature then.
Step 3: inoculation fermentation, inoculation 1500 ml genus bacillus in above-mentioned liquid medium, regulator solution temperature to 31 ℃, pH value is 4; Stir 120r/min, aerobic culture 48h grows gemma until thalline, puts into fermentor tank then; Leavening temperature is 34 ℃, and fermentation time is 30h, and the pH value scope is 5.5.
Claims (2)
1. used for aquiculture microbial preparation is characterized in that: it is the product that genus bacillus is fermented in the substratum of being made up of glucose, sucrose, steeping water, potassium primary phosphate and seawater and obtains.
2. the preparation method of a used for aquiculture microbial preparation is characterized in that: it is according to following steps preparation, step 1: the preparation of substratum; The component ratio of substratum is: glucose 1～1.5g; Sucrose 2～5g, steeping water 3～5g, potassium primary phosphate 0.02～0.05g; Seawater 1000ml, pH value are 4.8～5.5;
Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature promptly utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, cool the temperature to normal temperature then;
Step 3: inoculation fermentation, in above-mentioned liquid medium, inoculate 1200～1500 ml genus bacillus, regulator solution temperature to 29～31 ℃; PH value is 4～7, stirs 100～120r/min, aerobic culture 36～48h; Grow gemma until thalline, put into fermentor tank then, leavening temperature is 28～34 ℃; Fermentation time is 25～30h, and the pH value scope is 4.8～5.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110165822 CN102329005B (en) | 2011-06-20 | 2011-06-20 | Preparation method of microbial preparation for aquaculture |
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|---|---|---|---|
| CN 201110165822 CN102329005B (en) | 2011-06-20 | 2011-06-20 | Preparation method of microbial preparation for aquaculture |
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| CN102329005A true CN102329005A (en) | 2012-01-25 |
| CN102329005B CN102329005B (en) | 2013-04-24 |
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| CN 201110165822 Expired - Fee Related CN102329005B (en) | 2011-06-20 | 2011-06-20 | Preparation method of microbial preparation for aquaculture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528386A (en) * | 2021-07-14 | 2021-10-22 | 大连金砣水产食品有限公司 | Preparation method of aquatic product composite microecological preparation |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW252035B (en) * | 1994-12-13 | 1995-07-21 | Sy-Wey Wang | Microbiological water stabilization and purification agent used in aquaculture |
| CN1340463A (en) * | 2000-08-25 | 2002-03-20 | 中国水产科学研究院黄海水产研究所 | Bacillus circulans method for biologically repairing culture environment of aquatic products |
| CN101078004A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof |
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| CN101412983A (en) * | 2008-11-20 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans, preparation of high-density cultivated spore preparation, and use thereof |
| US20090252848A1 (en) * | 2008-04-03 | 2009-10-08 | Sunanta Pongsamart | Immunomodulating polysaccharide gel from durian fruit-rind as additive in animal feed |
| CN101948893A (en) * | 2010-09-27 | 2011-01-19 | 郑州奇泓生物科技有限公司 | Method for producing nisin by continuous fermentation |
| CN101993843A (en) * | 2010-08-17 | 2011-03-30 | 中国水产科学研究院黄海水产研究所 | Bacillus firmus strain and application thereof |
-
2011
- 2011-06-20 CN CN 201110165822 patent/CN102329005B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW252035B (en) * | 1994-12-13 | 1995-07-21 | Sy-Wey Wang | Microbiological water stabilization and purification agent used in aquaculture |
| CN1340463A (en) * | 2000-08-25 | 2002-03-20 | 中国水产科学研究院黄海水产研究所 | Bacillus circulans method for biologically repairing culture environment of aquatic products |
| CN101078004A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof |
| CN101331881A (en) * | 2007-06-27 | 2008-12-31 | 国家海洋局第一海洋研究所 | Sea bacillus wettable powder, and preparation method and use thereof |
| US20090252848A1 (en) * | 2008-04-03 | 2009-10-08 | Sunanta Pongsamart | Immunomodulating polysaccharide gel from durian fruit-rind as additive in animal feed |
| CN101412983A (en) * | 2008-11-20 | 2009-04-22 | 江苏省苏微微生物研究有限公司 | Bacillus coagulans, preparation of high-density cultivated spore preparation, and use thereof |
| CN101993843A (en) * | 2010-08-17 | 2011-03-30 | 中国水产科学研究院黄海水产研究所 | Bacillus firmus strain and application thereof |
| CN101948893A (en) * | 2010-09-27 | 2011-01-19 | 郑州奇泓生物科技有限公司 | Method for producing nisin by continuous fermentation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113528386A (en) * | 2021-07-14 | 2021-10-22 | 大连金砣水产食品有限公司 | Preparation method of aquatic product composite microecological preparation |
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