CN102329005B - Preparation method of microbial preparation for aquaculture - Google Patents

Abstract

The invention provides a microbial preparation for aquaculture and a preparation method thereof. The microbial preparation is a product obtained by fermenting bacillus in a culture medium consisting of glucose, saccharose, corn steep liquor, monopotassium phosphate and seawater. The preparation method comprises the steps of culture medium preparation, high-temperature sterilization and inoculating fermentation. The microbial preparation has the advantages of regulating water quality substrate, improving culture environment, promoting growth, preventing diseases and the like.

Description

A kind of preparation method of used for aquiculture microbial preparation

Technical field

The invention belongs to aquaculture field, especially belong to a kind of preparation method of used for aquiculture microbial preparation.

Background technology

Along with the fast development of culture fishery, particularly greatly develop today of batch production high-density breeding, cultivate good breeding environment, enhance productivity, reducing production costs becomes the essential condition of aquaculture.Objectionable impurities in holothruian cultures field degraded water body, improve seawater quality and play particularly important effect for the growth of sea cucumber, and existing for used for aquiculture the microbial preparation kind seldom, the microbial preparation that uses for sea-foods such as being fit to sea cucumber is famine especially, some aquaculture enterprises drop into antibiotics in the culturing pool at present, microbiotic only plays the effect of sterilization, and long-time the use can be reduced water quality, affects the growth of sea cucumber.

Summary of the invention

The object of the present invention is to provide a kind of regulating water quality substrate, improve breeding environment, promote growth, preventing disease, low, effective used for aquiculture microbial preparation and the preparation method of cost.

Used for aquiculture microbial preparation and preparation method that the present invention proposes, it is the product that genus bacillus is fermented in the substratum that is comprised of glucose, sucrose, corn steep liquor, potassium primary phosphate and seawater and obtains.

Its preparation process is as follows:

Step 1: the preparation of substratum, the component ratio of substratum is: glucose 1～1.5g, sucrose 2～5g, corn steep liquor 3～5g, potassium primary phosphate 0.02～0.05g, seawater 1000ml, the pH value is 4.8～5.5.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature namely utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, then cool the temperature to normal temperature.

Step 3: inoculation fermentation, in above-mentioned liquid medium, inoculate 1200～1500 ml genus bacillus, regulate liquid temperature to 29～31 ℃, pH value is 4～7, stirring 100～120r/min, aerated culture 36～48h, until thalline grows gemma, then put into fermentor tank, leavening temperature is 28～34 ℃, fermentation time is 25～30h, and pH value scope is 4.8～5.5.

Usage: this preparation directly dropped in the culturing pool by 1/100000 consumption get final product.

The present invention compared with prior art has following advantage:

1, the objectionable impuritiess such as the ammonia nitrogen in the degrading cultivation water, nitrite are improved breeding environment;

2, adapt to efficiently, fast sea water culture environment, prolong and change the water time;

3, preventing disease, the growth of promotion sea cucumber improve output.

Description of drawingsFig. 1 is that aquaculture water uses preparation of the present invention and the comparison diagram that does not use preparation ammonia nitrogen amount of the present invention to reduce.

EmbodimentExample 1, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1g, sucrose 2g, corn steep liquor 3g, potassium primary phosphate 0.02g, seawater 1000ml, the pH value is 4.8.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110 ℃ of real disappearing of high temperature namely utilize steam that substratum and fermentor tank are heated to 110 ℃, keep 20min, then cool the temperature to normal temperature.

Step 3: inoculation fermentation, inoculation 1200 ml genus bacillus in above-mentioned liquid medium are regulated liquid temperature to 29 ℃, and the pH value is 4, stir 100r/min, aerated culture 36h until thalline grows gemma, then puts into fermentor tank, leavening temperature is 28 ℃, and fermentation time is 25h, pH value 4.8.

The preparation that obtains according to above step is fed in 500/jin the sea cucumbers culturing pool, the ammonia nitrogen quantitative changeization of the aquaculture water in ten days as shown in Figure 1, the ■ line represents to use the variable quantity of ammonia nitrogen amount of the present invention among Fig. 1, ▲ line is expressed as the variable quantity that does not use ammonia nitrogen amount of the present invention, according to shown in Figure 1, the present invention has and significantly falls the ammonia nitrogen effect.Except falling ammonia nitrogen, the present invention also has certain promoter action to the growth of seedling, and shown in following form, this form is for using the present invention and the rate of increase table that does not use seedling of the present invention:

Shown in form, use the present invention can obviously increase the growth of seedling.

Example 2, step 1: the preparation of substratum, the component ratio of substratum is: glucose 1.5g, sucrose 5g, corn steep liquor 5g, potassium primary phosphate 0.05g, seawater 1000ml, the pH value is 5.5.

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 121 ℃ of real disappearing of high temperature namely utilize steam that substratum and fermentor tank are heated to 121 ℃, keep 30min, then cool the temperature to normal temperature.

Step 3: inoculation fermentation, inoculation 1500 ml genus bacillus in above-mentioned liquid medium are regulated liquid temperature to 31 ℃, and the pH value is 4, stir 120r/min, aerated culture 48h until thalline grows gemma, then puts into fermentor tank, leavening temperature is 34 ℃, and fermentation time is 30h, and pH value scope is 5.5.

Claims (1)

1. the preparation method of a used for aquiculture microbial preparation, it is characterized in that: described microbial preparation is the product that genus bacillus is fermented in the substratum that is comprised of glucose, sucrose, corn steep liquor, potassium primary phosphate and seawater and obtains, and this microbial preparation prepares according to following steps:

Step 1: the preparation of substratum, the component ratio of substratum is: glucose 1～1.5g, sucrose 2～5g, corn steep liquor 3～5g, potassium primary phosphate 0.02～0.05g, seawater 1000ml, the pH value is 4.8～5.5;

Step 2: high temperature is real to disappear, and above-mentioned liquid medium is put into fermentor tank, and 110～121 ℃ of real disappearing of high temperature namely utilize steam that substratum and fermentor tank are heated to 110～121 ℃, keep 20～30min, then cool the temperature to normal temperature;

Step 3: inoculation fermentation, in above-mentioned liquid medium, inoculate 1200～1500 ml genus bacillus, regulate liquid temperature to 29～31 ℃, pH value is 4～7, stirring 100～120r/min, aerated culture 36～48h, until thalline grows gemma, then put into fermentor tank, leavening temperature is 28～34 ℃, fermentation time is 25～30h, and pH value scope is 4.8～5.5.

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