CN103343105A - Preparation method of novel liquid pit mud - Google Patents
Preparation method of novel liquid pit mud Download PDFInfo
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- CN103343105A CN103343105A CN2013102998408A CN201310299840A CN103343105A CN 103343105 A CN103343105 A CN 103343105A CN 2013102998408 A CN2013102998408 A CN 2013102998408A CN 201310299840 A CN201310299840 A CN 201310299840A CN 103343105 A CN103343105 A CN 103343105A
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Abstract
The invention relates to a preparation method of novel liquid pit mud. The method comprises the following steps of: 1) performing a grading enrichment culture on pit mud bacteria; 2) strengthening pit mud functional bacteria; and 3) mixing a pit mud enrichment culture solution with the pit mud functional bacteria. The method is characterized in that the prepared pit mud which is in a liquid state and small in viscosity is directly sprayed into fermented grains through a spraying device when being used, so that not only is the contact area between microorganisms and the fermented grains is enlarged but also the problems of the falling and the aging of the traditional solid pit mud are avoided. Thus, the method is favorable for the development of wine brewing mechanization.
Description
Technical field
The present invention relates in a kind of brewed spirit the making method of cellar for storing things mud, specifically be the making method of a kind of liquid cellar for storing things mud.
Background technology
Traditional liquor is brewageed the solid-state cellar for storing things mud that adopt more, and the status of mud in brewed spirit, cellar for storing things is self-evident, and the quality of cellar for storing things mud is directly determining the quality of vinosity.Aroma type daqu liquor particularly, its main body fragrance matter is ethyl hexanoate, and ethyl hexanoate to be that clostridium (caproic acid bacteria) etc. is various in the mud of cellar for storing things give birth to the fragrant meta-bolitess that produce the ester microorganisms, so there not be cellar for storing things mud well, just can not produce superior Luzhou-flavor quality liquor." a century is stored always " gone out wine this reason just.But nowadays, some producers degenerate because of cellar for storing things mud and cause vinosity poor, and cost is high and be forced to stop production.Jiao Chi is aging, white particle and white, needle-shaped crystals occur, causes former wine ethyl hexanoate content to reduce.Cellar for storing things mud wears out serious position major part in the Jiao Chi middle and upper part, and the fragrance of smelling is little, is to cause the major cause that Luzhou-flavor quality liquor rate is low, cost is high.Each brewery drops into expense every year aspect the Jiao Chi maintenance proportion is also more and more higher.And be mechanize the tomorrow of brewed spirit, and conventional solid-state cellar for storing things mud easily comes off in mechanize is brewageed, and is seriously restricting the mechanize production of liquor.
Use the liquid cellar for storing things of the present invention mud, not only solved the aging of cellar for storing things mud, the problem that comes off more is conducive to the development of liquor mechanize.And the liquor of brewageing remains basically stable with traditional fermentation aspect quality, also slightly surpasses.
Summary of the invention
Technical problem solved by the invention is to make a kind of cellar for storing things mud of liquid state, and is aging in brewageing to solve tradition cellar for storing things mud, the problem that comes off, and more can be applicable to the production of modern liquor mechanize.
Technical problem solved by the invention realizes by the following technical solutions:
The making method of a kind of novel cellar for storing things mud is characterized in that, may further comprise the steps:
The first step: the classification enrichment culture of cellar for storing things mud bacterium;
Second step: pit mud functional bacteria is strengthened;
The 3rd step: the use that mixes of cellar for storing things mud enrichment culture liquid and pit mud functional bacteria.
The cultivation of cellar for storing things mud bacterium divides level Four to cultivate.
One or two three-stage culture mediums were during cellar for storing things mud bacterium classification was cultivated: peptone 1wt%, and extractum carnis 0.3wt%, sodium-chlor 0.5wt%, SODIUMNITRATE 0.3wt%, all the other are water.Wherein the one-level cultivation is 35 ℃ of constant temperature culture 3～5 days; Two or three grades of cultivations are 35 ℃ and cultivated 2～3 days.
The level Four culture medium is: Semen Maydis powder 15wt%, give birth to bent powder 20wt%, and all the other are water.Press the material-water ratio gelatinization of 1:4, the material after the gelatinization is cooled to 60 ℃ and added the saccharification enzyme glycolysis 30 minutes, is cooled to 30 ℃, and value transfers to 7.0, connects three grades of seeds, and inoculum size is 10%(v/v), seal with plastic film, cultivated 5 days for 35 ℃.
Mud function yeast of the present invention comprises caproic acid bacteria, yeast and methanobacteria.
The bacterial classification source is Hubei University Of Technology's preservation bacterial classification.
The cultivation of caproic acid bacteria, yeast and methanobacteria all is divided into Pyatyi and cultivates.
The caproic acid bacteria that the cellar for storing things mud enrichment culture liquid that the liquid cellar for storing things of the present invention mud the first step is cultivated and second step cultivate, the proportioning of yeast nutrient solution is 5:4:1 (v/v), and wherein methanobacteria uses separately, and consumption is 5%～10% (v/v).
Beneficial effect: the present invention draws materials under scientific and reasonable prerequisite conveniently, has also avoided unnecessary waste simultaneously, economizes on resources, and reduces cost, and is beneficial to wide popularization and application.The more important thing is that industry situation of the present invention cellar for storing things mud can not wear out, come off, and easy to use, be conducive to the development of present mechanize brewing wine.
Description of drawings
Fig. 1 is the process flow diagram of specific embodiments of the present invention.
Embodiment
The method of manufacturing employing " progressively classification enlarged culturing " of the liquid cellar for storing things of the present invention mud.Mainly be cellar for storing things mud bacterium (mainly be bacterium, bacillus also has a small amount of mould), the classification of pit mud functional bacteria (yeast, caproic acid bacteria and methanobacteria) is cultivated.
The production of the liquid cellar for storing things of the present invention mud divided for three steps carried out following step-by-step instructions:
The embodiment the first step: the classification of pit mud functional bacteria (Zhijiang wine industry in Hubei Province's is former in cellar for storing things mud) is cultivated.
1) one-level medium component: peptone 1wt%, extractum carnis 0.3wt%, sodium-chlor 0.5wt%, SODIUMNITRATE 0.3wt%, all the other are water, with NaOH solution pH are transferred to about 7.0.To be dissolved in the 50ml water from the cellar for storing things mud 5g that Zhijiang wine is already fetched, make cellar for storing things mud bacterium liquid, get 10ml and join in the above-mentioned substratum, 35 ℃ of constant temperature culture 3～5 days.Cultivate and finish back selection fermentation
Vigorous, microscopy bacillus triangular flask many and strong shape is made original strain.
2) two or three grades of enlarged culturing substratum are identical with one-level, received by the 250ml triangular flask
The 1000ml triangular flask is cultivated, and receives the 3000ml triangular flask again, and inoculum size is 10%(v/v), cultivated 2～3 days with the plug of pipe beyond the Great Wall for 35 ℃.
3) the level Four culture medium is: Semen Maydis powder 15wt%, and fecula 20wt%, all the other are water.Press the material-water ratio gelatinization of 1:4, the material after the gelatinization is cooled to 60 ℃ and added the saccharification enzyme glycolysis 30 minutes, is cooled to 30 ℃ of values and transfers to 7.0, connects three grades of seeds, and inoculum size is 10%(v/v), seal with plastic film, cultivated 5 days for 35 ℃.
Second step of embodiment:
1, the reinforcement of pit mud functional bacteria---caproic acid bacteria:
1) cultivation of caproic acid bacteria, bacterial classification are that 30# shuttle shape bud pole bacterium is gently ground in Hubei University Of Technology preservation Inner Mongol, and substratum adopts Pasteur's culture medium.
2) first order seed is cultivated, and the Inner Mongol is gently ground the 30# clostridium and inserted test tube, 35 ℃ of constant temperature cultivations 5 days.
3) secondary seed is cultivated, and cultured first order seed is inserted in the 150ml triangular flask that has " S " type tubule vacuum tightness 79999.2Pa, 35 ℃ of constant temperature culture 7 days respectively.
4) three grades of seed culture, medium component: sodium acetate 0.5wt%, ammonium sulfate 0.05wt%, sal epsom 0.02wt%, potassium primary phosphate 0.02wt%, yeast extract paste 0.1wt%, ethanol 2%(v/v) (after the sterilization, adding before the inoculation), all the other are water.With the substratum 500ml triangular flask of packing into of sterilization, inoculum size 10%(v/v), with the plug of vapor pipe, cultivated 7 days for 35～37 ℃ beyond the Great Wall.
5) level Four is cultivated, medium component: high-quality yellow water 40%～50% (v/v), and sodium-acetate 0.3wt%, all the other are water.With 6.5～7.0 of sodium bicarbonate adjust pH, the sterilization back adds ethanol 2% (v/v).With the sterilized substratum 3000ml triangular flask of packing into, inoculum size 10% (v/v) with the plug of vapor pipe, was cultivated 7 days for 35～37 ℃ beyond the Great Wall.
6) Pyatyi is cultivated, and substratum at the corresponding levels is identical with the higher level, with pack into the little altar of 25～50kg of sterilized substratum, and inoculum size 10% (v/v), with the oilpaper sealing, 35～37 ℃ of cultivations 7 days.
2, pit mud functional bacteria---saccharomycetic reinforcement:
1) bacterial classification: Hubei University Of Technology's preservation ester-producing yeast bacterium bacterial classification.
2) one-level is cultivated, and substratum (YPDA) is: 2wt% peptone, 1wt% yeast extract, 0.2wt% glucose, 0.2% adenine solution of 1.5% (v/v), 2wt% agar.Melt the packing test tube, sterilized 30 minutes, inoculation one ring in cooling back was cultivated 48 hours.
3) the secondary liquid tube is cultivated, YPDA (not adding agar), and sterilization finishes the back and inserts slant strains, and inoculum size is 3 rings, cultivates 48 hours for 28～30 ℃.
4) three grades of triangular flasks are cultivated, and substratum (PDA) is 20% potato filtrate, glucose 2wt%.With the substratum 1000ml triangular flask of packing into, liquid tube is inserted in sterilization cooling back, and inoculum size 10% (v/v) was cultivated 24 hours for 28～30 ℃.
5) the level Four fastening tank is cultivated, and substratum is the PDA substratum, cultivates 10～12 hours for 28～30 ℃.
6) the Pyatyi vat is cultivated, and substratum at the corresponding levels is cultivated identical with method and fastening tank.
3, pit mud functional bacteria---methanobacteria is cultivated:
1) bacterial classification is Hubei University Of Technology's preservation cloth formula methagen.
2) cultivating the tomb composition is sodium-acetate 1.11wt%, K
HPO
0.05wt%, NH
Cl
0.075wt%, ethanol 2% (v/v), Oxygen Scavenger (Na
S 1wt% and Na
CO
5wt% mixes
Close) 3%, pH6.8～7.0 sterilization 30 minutes, cooling back inoculation culture, inoculum size 16% (v/v) seals bottleneck beyond the Great Wall with the plug of vapor pipe, and with paraffin, 35 ℃ of anaerobic culture 7～10 days, the method for enlarged culturing and medium component are cultivated with one-level step by step.
The 3rd step of embodiment: the caproic acid bacteria that the cellar for storing things mud enrichment culture liquid that the liquid cellar for storing things of the present invention mud the first step is cultivated and second step cultivate, the proportioning of yeast nutrient solution is 5:4:1 (v/v), and wherein methanobacteria uses separately, and consumption is 5%～10% (v/v).
Above-mentioned primary process of the present invention and the method for having shown, but the present invention is not restricted to the described embodiments, above-described embodiment and explanation just illustrate primary process of the present invention and method, under the premise without departing from the spirit and scope of the present invention, the present invention can also have various improvement, all still belongs to technical scheme of the present invention.
Claims (4)
1. the making method of a novel liquid cellar for storing things mud is characterized in that, may further comprise the steps:
The first step: the classification enrichment culture of cellar for storing things mud bacterium;
Second step: pit mud functional bacteria is strengthened;
The 3rd step: the use that mixes of cellar for storing things mud enrichment culture liquid and pit mud functional bacteria.
2. a kind of novel liquid state according to claim 1 is stored the making method of mud, it is characterized in that:
(1) cultivation of cellar for storing things mud bacterium divides level Four to cultivate;
(2) one or two three-stage culture mediums were during the classification of cellar for storing things mud bacterium was cultivated: peptone 1wt%, and extractum carnis 0.3wt%, sodium-chlor 0.5wt%, SODIUMNITRATE 0.3wt%, all the other are water; Wherein the one-level cultivation is 35 ℃ of constant temperature culture 3～5 days, and two or three grades of cultivations are 35 ℃ and cultivated 2～3 days;
(3) the level Four culture medium is: Semen Maydis powder 15wt%, give birth to bent powder 20wt%, and all the other are water; Press the material-water ratio gelatinization of 1:4, the material after the gelatinization is cooled to 60 ℃ and added the saccharification enzyme glycolysis 30 minutes, is cooled to 30 ℃, and value transfers to 7.0, connects three grades of seeds, and inoculum size is 10% (v/v), seals with plastic film, cultivates 5 days for 35 ℃.
3. a kind of novel liquid state according to claim 1 is stored the making method of mud, it is characterized in that:
(1) pit mud functional bacteria comprises caproic acid bacteria, yeast and methanobacteria;
(2) the bacterial classification source is Hubei University Of Technology's preservation bacterial classification;
(3) cultivation of caproic acid bacteria, yeast and methanobacteria all is divided into the Pyatyi cultivation.
4. the making method of a kind of novel cellar for storing things according to claim 1 mud, its feature exists
In, the caproic acid bacteria that the cellar for storing things mud enrichment culture liquid that the first step is cultivated and second step cultivate, the proportioning of yeast nutrient solution is 5:4:1 (v/v), and wherein methanobacteria uses separately, and consumption is 5%～10% (v/v).
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666927A (en) * | 2013-12-20 | 2014-03-26 | 达州市川虎食品有限公司 | Process for preparing fresh scent strong-flavor Chinese spirit |
| CN103897920A (en) * | 2014-02-26 | 2014-07-02 | 泰山酒业集团股份有限公司 | Preparation method of space caproic acid bacteria pit mud for brewing wine |
| CN104694324A (en) * | 2014-03-05 | 2015-06-10 | 江苏乾天酒业有限公司 | Pit mud preparation process |
| CN105199898A (en) * | 2015-09-25 | 2015-12-30 | 安徽乐天酿酒有限公司 | Artificial culture method of pit mud |
| CN106010886A (en) * | 2016-08-11 | 2016-10-12 | 四川省宜宾市叙府酒业股份有限公司 | Method for producing pit mud and application method thereof |
| CN111440838A (en) * | 2020-03-25 | 2020-07-24 | 北京科技大学 | Method for producing hexanoic acid by fermenting white spirit vinasse by enriching hexanoic acid functional bacteria in pit mud |
| CN111500507A (en) * | 2020-05-28 | 2020-08-07 | 江南大学 | Pit mud flora enrichment method based on oligoculture and application thereof |
| CN112011419A (en) * | 2020-08-14 | 2020-12-01 | 江南大学 | Caproic acid bacteria-rich nutrient solution and preparation method and application thereof |
-
2013
- 2013-07-17 CN CN2013102998408A patent/CN103343105A/en active Pending
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| 孙陈浪 等: "窖泥中重要微生物的分析研究与多菌种共酵培养液态窖泥的实践应用", 《酿酒科技》 * |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666927A (en) * | 2013-12-20 | 2014-03-26 | 达州市川虎食品有限公司 | Process for preparing fresh scent strong-flavor Chinese spirit |
| CN103666927B (en) * | 2013-12-20 | 2015-07-01 | 达州市川虎食品有限公司 | Process for preparing fresh scent strong-flavor Chinese spirit |
| CN103897920A (en) * | 2014-02-26 | 2014-07-02 | 泰山酒业集团股份有限公司 | Preparation method of space caproic acid bacteria pit mud for brewing wine |
| CN103897920B (en) * | 2014-02-26 | 2016-04-06 | 泰山酒业集团股份有限公司 | A kind of wine brewing stores the preparation method of mud with space caproic acid bacteria |
| CN104694324A (en) * | 2014-03-05 | 2015-06-10 | 江苏乾天酒业有限公司 | Pit mud preparation process |
| CN105199898A (en) * | 2015-09-25 | 2015-12-30 | 安徽乐天酿酒有限公司 | Artificial culture method of pit mud |
| CN106010886A (en) * | 2016-08-11 | 2016-10-12 | 四川省宜宾市叙府酒业股份有限公司 | Method for producing pit mud and application method thereof |
| CN111440838A (en) * | 2020-03-25 | 2020-07-24 | 北京科技大学 | Method for producing hexanoic acid by fermenting white spirit vinasse by enriching hexanoic acid functional bacteria in pit mud |
| CN111500507A (en) * | 2020-05-28 | 2020-08-07 | 江南大学 | Pit mud flora enrichment method based on oligoculture and application thereof |
| CN112011419A (en) * | 2020-08-14 | 2020-12-01 | 江南大学 | Caproic acid bacteria-rich nutrient solution and preparation method and application thereof |
| CN112011419B (en) * | 2020-08-14 | 2022-11-11 | 江南大学 | Caproic acid bacteria-rich nutrient solution and preparation method and application thereof |
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Application publication date: 20131009 |