CN102952834B - Method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus - Google Patents
Method for producing microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus Download PDFInfo
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- CN102952834B CN102952834B CN201210425106.7A CN201210425106A CN102952834B CN 102952834 B CN102952834 B CN 102952834B CN 201210425106 A CN201210425106 A CN 201210425106A CN 102952834 B CN102952834 B CN 102952834B
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Abstract
The present invention belongs to microbial polysaccharide fermentation, and particularly relates to a method for producing a microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus, wherein Paenibacillus mucilaginosus is inoculated in a culture medium containing a carbon source and a nitrogen source to carry out fermentation, and bacteria heat stimulation, addition of a surfactant during a fermentation process, and other manners are performed to optimize the production process. With the present invention, the problem of low yield of polysaccharides in the fermentation broth in the prior art is solved, and advantages of high polysaccharide yield in the fermentation broth, good bacterial growth, short fermentation period and the like are provided.
Description
Technical field
The invention belongs to the fermentation of microbial polysaccharide, refer to especially a kind of method of utilizing colloid series bacillus to produce microbial polysaccharide fermentation liquid.
Background technology
Gel-shaped series bacillus is Soil Bacillus, and its bacterium colony is circular, colourless, protuberance, and colloid thickness, transparent or semitransparent, neat in edge.The major function bacterial strain that is used as in the past few decades microbial fertilizer is exploited, and it has brought into play vital role in agricultural microorganism formulation art.Along with deepening continuously that colloid series bacillus is studied, people start to note research and the application of its exocellular polysaccharide in recent years.But focus still concentrates on analysis and the capacity of decomposition aspect of polysaccharide to silicate minerals of colloid bacillus cereus being produced to polysaccharide component.Research to the new Application Areas of its polysaccharide and Optimization Technology increase polysaccharide yield aspect is also less.The traditional technology that people produce microbial polysaccharide is at present mainly switching inclined plane method activated inclined plane bacterial classification, optimizes the conventional steps such as shake flask fermentation processing parameter, and utilizing the yield level of the polysaccharide in fermentation liquid of aforesaid method production is 3-5g/L.
Summary of the invention
The object of the present invention is to provide the high colloid series bacillus that utilizes of microbial polysaccharide content in a kind of fermented liquid to produce the method for microbial polysaccharide fermentation liquid.
Overall technology design of the present invention is:
Utilize colloid series bacillus to produce the method for microbial polysaccharide fermentation liquid, be colloid series bacillus (Paenibacillus mucilaginosus) ACCC10013 to be inoculated in to the substratum that contains carbon source and nitrogenous source ferment, comprise following processing step:
A, bacterial classification thermal stimulus
Bacterial classification spore in aseptic condition Xia Jiang test tube slant adds sterilized water, vibration is washed lower spore and is made spore suspension and be placed in sterile chamber, the sterile chamber that spore suspension is housed is placed in to boiling water, and homogeneous heating, after 1-10 minute takes out sterile chamber also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 5%-20%, sucrose 5%-10%, ammonium sulfate 0.5%-5%, dipotassium hydrogen phosphate 10%-20%, sal epsom 5%-10%, iron trichloride 0.01%-0.5%, calcium carbonate 0.1%-5%, yeast extract paste 0.1%-3%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: (ventilating ratio is fermentating liquid volume to 0-4 hour ventilating ratio: per minute passes into the volume of air, lower same.) be 1:0.2-0.5VVM, 4-12 hour ventilating ratio is 1:0.5-0.7VVM, 12-20 hour ventilating ratio is 1:0.7-0.9VVM, 20-36 hour ventilating ratio is 1:1.1-1.5VVM, 28-36 hour ventilating ratio is 1:0.5-0.7VVM, 36-60 hour ventilating ratio is 1:0.3-0.5VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 1-10ml.
Concrete technology contents in the present invention also has:
Sterile chamber can be selected including, but not limited to test tube or other and be convenient to the container of heating, and for ease of the heating of sterile chamber, preferred technical scheme is that the sterile chamber in steps A is selected test tube.
Homogeneous heating can adopt multiple existing mode, does not all depart from essence of the present invention, and wherein comparatively preferred technical scheme is, the homogeneous heating in steps A adopts the mode of limit heating edge vibration to carry out.
It is to adopt cold water that sterile chamber is cooling that sterile chamber is taken out also cooling rapidly.
In step B, charge amount is 38 liters of 50 liters of canister chargings.
The substantive distinguishing features that the present invention possesses and significant technical progress are:
1, by the bacterial classification violet staining after thermal stimulus, smear, microscopy finds that weak bacterium and the gemma that some are weak have reduced, gemma is more neat, painted darker, and the performance of fermenting on 50 liters of canisters is that gemma is sprouted soon, the delayed growth phase shortens, reduced total fermentation time, and thalli growth synchronism is conducive to well the technology controlling and process of back, such as adding of tensio-active agent.
2, colloid series bacillus produces a large amount of pod membranes when nutrition is poor, its 5-10 that can reach thalline size doubly, the method that the present invention has taked wave band to cultivate during the fermentation, in the early stage of cultivating to the good growing environment of bacterial classification to improve its biomass, in the later stage of fermentation culture, the strict dissolved oxygen of controlling also adds tensio-active agent, creates the permeability that oligotrophic environment changes cytolemma simultaneously and makes it at a large amount of accumulated polysaccharides of fermented liquid, to reach the object that improves polysaccharide yield.
3,, after fermentation ends, measure polysaccharide content.Detection method is anthrone colorimetry, and its content reaches as high as 7.5g/L, can improve output of sugar 10-50% with the comparison of current report technique.
4, the fermented liquid that fermentation produces carries out preliminary extraction after separating by sevag method and carries out UV scanning detection, detects and finds that it is not substantially containing nucleic acid and protein.And tentatively record it and contain abundant hydroxyl and carboxyl, and the viscosity of this polysaccharide is high, similar with viscosity of xanthan gums, and it has good pseudo-plasticity and suspension, apply it to high-grade ceramic and make above, find that seldom this polysaccharide of amount can produce good effect, improve the smooth finish of ceramic surface, improve yield rate, improving product class, can produce good economic benefit.But the fermentation time with respect to this polysaccharide of xanthan gum is short, the fermentation later stage is less to the demand of oxygen, has reduced the aeration-agitation power consumption causing due to fermentation broth viscosity increase, saves special equipment requirements.But good field has been started in this application for colloid bacillus cereus product polysaccharide.In addition, due to its high viscosity having, the characteristics such as pseudo-plasticity and suspension, it can also be in petroleum prospecting, and also there is good application prospect mineral floating aspect.
Embodiment
Below in conjunction with embodiments of the invention, do further and describe; but not as a limitation of the invention; the content that protection scope of the present invention is recorded with claim is as the criterion, and any equivalence techniques means of making according to specification sheets are replaced, and all do not depart from protection scope of the present invention.
Embodiment 1
Utilize colloid series bacillus to produce the method for microbial polysaccharide fermentation liquid, be colloid series bacillus (Paenibacillus mucilaginosus) ACCC10013 to be inoculated in to the substratum that contains carbon source and nitrogenous source ferment, comprise following processing step:
A, bacterial classification thermal stimulus
Bacterial classification spore in aseptic condition Xia Jiang test tube slant adds sterilized water, vibration is washed lower spore and is made spore suspension and be placed in sterile chamber, the sterile chamber that spore suspension is housed is placed in to boiling water, and homogeneous heating, after 1 minute takes out sterile chamber also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 5%, sucrose 5%, ammonium sulfate 0.5%, dipotassium hydrogen phosphate 10%, sal epsom 5%, iron trichloride 0.01%, calcium carbonate 0.1%, yeast extract paste 0.1%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:0.2VVM, 4-12 hour ventilating ratio is 1:0.5VVM, 12-20 hour ventilating ratio is 1:0.7VVM, 20-36 hour ventilating ratio is 1:1.1VVM, 28-36 hour ventilating ratio is 1:0.5VVM, 36-60 hour ventilating ratio is 1:0.3VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 1ml.
Sterile chamber in steps A is selected test tube.
Homogeneous heating in steps A adopts the mode of limit heating edge vibration to carry out.
It is to adopt cold water that sterile chamber is cooling that sterile chamber is taken out also cooling rapidly.
In step B, charge amount is 38 liters of 50 liters of canister chargings.
After fermentation ends, adopt the polysaccharide content in anthrone colorimetric method for determining fermented liquid.Its content can reach 6.85g/L.
Embodiment 2
In the present embodiment, in steps A, sterile chamber homogeneous heating, after 10 minutes, is taken out sterile chamber also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 20%, sucrose 10%, ammonium sulfate 5%, dipotassium hydrogen phosphate 20%, sal epsom 10%, iron trichloride 0.5%, calcium carbonate 5%, yeast extract paste 3%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:.5VVM, 4-12 hour ventilating ratio is 1:0.7VVM, 12-20 hour ventilating ratio is 1:0.9VVM, 20-36 hour ventilating ratio is 1:1.5VVM, 28-36 hour ventilating ratio is 1:0.7VVM, 36-60 hour ventilating ratio is 1:0.5VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 10ml.
All the other contents are with embodiment 1.
After fermentation ends, adopt the polysaccharide content in anthrone colorimetric method for determining fermented liquid.Its content can reach 7.2g/L.
Embodiment 3
The step of the present embodiment is placed in boiling water homogeneous heating by the sterile chamber that spore suspension is housed, and after 8 minutes, sterile chamber is taken out also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 15%, sucrose 8%, ammonium sulfate 3%, dipotassium hydrogen phosphate 15%, sal epsom 8%, iron trichloride 0.3%, calcium carbonate 3%, yeast extract paste 1%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:0.3VVM, 4-12 hour ventilating ratio is 1:0.6VVM, 12-20 hour ventilating ratio is 1:0.8VVM, 20-36 hour ventilating ratio is 1:1.3VVM, 28-36 hour ventilating ratio is 1:0.6VVM, 36-60 hour ventilating ratio is 1:0.4VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 8ml.
All the other contents are with embodiment 1.
After fermentation ends, adopt the polysaccharide content in anthrone colorimetric method for determining fermented liquid.Its content can reach 7.5g/L.
Embodiment 4
The step of the present embodiment is placed in boiling water homogeneous heating by the sterile chamber that spore suspension is housed, and after 6 minutes, sterile chamber is taken out also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 8%, sucrose 6%, ammonium sulfate 1%, dipotassium hydrogen phosphate 13%, sal epsom 7%, iron trichloride 0.1%, calcium carbonate 1%, yeast extract paste 0.5%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:0.3VVM, 4-12 hour ventilating ratio is 1:0.6VVM, 12-20 hour ventilating ratio is 1:0.8VVM, 20-36 hour ventilating ratio is 1:1.3VVM, 28-36 hour ventilating ratio is 1:0.6VVM, 36-60 hour ventilating ratio is 1:0.5VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 3ml.
All the other contents are with embodiment 1.
After fermentation ends, adopt the polysaccharide content in anthrone colorimetric method for determining fermented liquid.Its content can reach 7.0g/L.
Embodiment 5
The step of the present embodiment is placed in boiling water homogeneous heating by the sterile chamber that spore suspension is housed, and after 3 minutes, sterile chamber is taken out also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 18%, sucrose 9%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 18%, sal epsom 9%, iron trichloride 0.07%, calcium carbonate 0.9%, yeast extract paste 0.8%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B, and fermentation period is 60 hours, 30 ℃ of culture temperature, and inoculum size is bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:0.4VVM, 4-12 hour ventilating ratio is 1:0.6VVM, 12-20 hour ventilating ratio is 1:0.85VVM, 20-36 hour ventilating ratio is 1:1.45VVM, 28-36 hour ventilating ratio is 1:0.6VVM, 36-60 hour ventilating ratio is 1:0.35VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 9ml.
All the other contents are with embodiment 1.
After fermentation ends, adopt the polysaccharide content in anthrone colorimetric method for determining fermented liquid.Its content can reach 6.95g/L.
Claims (5)
1. utilize colloid series bacillus to produce the method for microbial polysaccharide fermentation liquid, be colloid series bacillus (Paenibacillus mucilaginosus) ACCC10013 to be inoculated in to the substratum that contains carbon source and nitrogenous source ferment, it is characterized in that comprising following processing step:
A, bacterial classification thermal stimulus
Bacterial classification spore in aseptic condition Xia Jiang test tube slant adds 6ml sterilized water, vibration is washed lower spore and is made spore suspension and be placed in sterile chamber, the sterile chamber that spore suspension is housed is placed in to boiling water, and homogeneous heating, after 1-10 minute takes out sterile chamber also cooling rapidly;
B, fermentation
The preparation of B1, substratum
Preparation substratum sterilizing, be cooled to 30 ℃, and substratum is comprised of the component of following mass percent:
Starch 5%-20%, sucrose 5%-10%, ammonium sulfate 0.5%-5%, dipotassium hydrogen phosphate 10%-20%, sal epsom 5%-10%, iron trichloride 0.01%-0.5%, calcium carbonate 0.1%-5%, yeast extract paste 0.1%-3%, surplus is sterilized water, pH=7.5;
B2, cultivation
Bacterial classification after processing in steps A is inoculated in substratum prepared by step B1, and fermentation period is 60 hours, 30 ℃ of culture temperature, the inoculum size of bacterial classification: after sterilizing, the mass percent of substratum is 8%; Air quantity is adjusted to: 0-4 hour ventilating ratio is 1:0.2-0.5VVM, 4-12 hour ventilating ratio is 1:0.5-0.7VVM, 12-20 hour ventilating ratio is 1:0.7-0.9VVM, 20-36 hour ventilating ratio is 1:1.1-1.5VVM, 28-36 hour ventilating ratio is 1:0.5-0.7VVM, 36-60 hour ventilating ratio is 1:0.3-0.5VVM, finishes to cultivate, and after cultivating 36 hours, feed supplement stream adds tensio-active agent sodium laurylsulfonate 1-10ml.
2. the method for utilizing colloid series bacillus to produce microbial polysaccharide fermentation liquid according to claim 1, is characterized in that the sterile chamber in described steps A is selected test tube.
3. the method for utilizing colloid series bacillus to produce microbial polysaccharide fermentation liquid according to claim 1, is characterized in that the homogeneous heating in described steps A adopts the mode of limit heating edge vibration to carry out.
4. according to the colloid series bacillus that utilizes described in any one in claim 1-3, produce the method for microbial polysaccharide fermentation liquid, it is characterized in that described sterile chamber is taken out and cooling be rapidly to adopt cold water that sterile chamber is cooling.
5. the method for utilizing colloid series bacillus to produce microbial polysaccharide fermentation liquid according to claim 1, is characterized in that in described step B, charge amount is 38 liters of 50 liters of canister chargings.
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| CN103194410B (en) * | 2013-04-04 | 2015-03-04 | 河北省微生物研究所 | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same |
| CN103642873B (en) * | 2013-12-05 | 2015-09-09 | 河北省微生物研究所 | Colloid series bacillus is adopted to produce the method for microbial flocculant |
| CN103740618B (en) * | 2013-12-31 | 2015-08-05 | 光明乳业股份有限公司 | One Bacillus species novel bacterial and cultural method thereof and application |
| CN113151038B (en) * | 2021-01-13 | 2022-10-11 | 广东省农业科学院农业资源与环境研究所 | Extracellular polysaccharide producing strain, method for producing extracellular polysaccharide by using strain and application of extracellular polysaccharide |
| CN113477408B (en) * | 2021-07-21 | 2022-08-02 | 东北大学 | Application of curdlan serving as inhibitor in iron ore reverse flotation in mineral processing field and application method |
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| CN102154154A (en) * | 2010-12-27 | 2011-08-17 | 河北鑫合生物化工有限公司 | Method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor |
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| CN102154154A (en) * | 2010-12-27 | 2011-08-17 | 河北鑫合生物化工有限公司 | Method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor |
Non-Patent Citations (4)
| Title |
|---|
| 胡秀芳等.胶质芽孢杆菌突变株021120的培养条件及发酵工艺优化.《中国生物工程杂志》.2007,第27卷(第9期),第58-62页. |
| 胶质类芽孢杆菌的表型及遗传多样性分析;薛爱琴;《中国优秀硕士学位论文全文数据库基础科学辑》;20120426;第2012卷(第6期);A006-150页 * |
| 胶质芽孢杆菌突变株021120的培养条件及发酵工艺优化;胡秀芳等;《中国生物工程杂志》;20070915;第27卷(第9期);第58-62页 * |
| 薛爱琴.胶质类芽孢杆菌的表型及遗传多样性分析.《中国优秀硕士学位论文全文数据库基础科学辑》.2012,第2012卷(第6期),A006-150页. |
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